CN102539363A - Determining method for total flavone content of blumea balsamifera - Google Patents
Determining method for total flavone content of blumea balsamifera Download PDFInfo
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Abstract
The invention discloses a determining method for the total flavone content of blumea balsamifera or blumea balsamifera extractive or materials having the same total flavone with the blumea balsamifera, relating to an ultraviolet-visible spectrophotometry. The determining method comprises the following steps of: preparing a gradient standard solution by using rutin or blumeatin (3, 3', 5, 7-tetramethyl-4'-methoxy flavanone) reference material, preparing a solution to be tested by using a blumea balsamifera medical material or the blumea balsamifera extractive, directly determining the absorbance of the gradient standard solution under the wavelength of 271nm, and establishing a standard curve; and determining the absorbance of the solution to be tested again, finding out the concentration of the solution to be tested from the standard curve, and finally working out the total flavone content of the blumea balsamifera medical material or extractive according to the dilution multiple of solution preparation. According to the determining method, an isoabsorptive point is adopted for absorbing wavelengths to determine the total contents of the two types of materials with different ultraviolet absorptions; and the determining method has the advantages of strong pertinence, accurate and reliable results, good accuracy, stability and repeatability, high degree of accuracy, easiness in control, simpleness and convenience in operation and can be used for the quality control of the blumea balsamifera medical material or extractive.
Description
Technical field
The invention belongs to the Chinese medicine analysis field; The method of quality control that relates to a kind of Chinese crude drug or extract; Be specifically related to a kind of determination of total flavonoids method that contains two flavonoids, particularly a kind of method of measuring general flavone content in the identical material of Blumea balsamifera class medicinal material or Herba Blumeae Balsamiferae extract or Blumea balsamifera total flavones composition.
Background technology
Blumea balsamifera is feverfew Blumea balsamifera Blumea balsamifera (L.) DC. dry aerial parts.Chinese medicine is claimed Balsamiferou Blumea Herb again.Main product in Guangxi, Guizhou.According to " Guangxi Chinese crude drug standard " (nineteen ninety version) record, Blumea balsamifera (Balsamiferou Blumea Herb) property " hot, bitter, temperature ", the effect of tool " invigorate blood circulation in the temperature, menstruation regulating, dispel rheumatism ".Modern pharmacodynamic study shows; Multiple effect [Duan Zhen such as that the flavone compound in the Blumea balsamifera has is anti-oxidant, anticancer, radioresistance and acute liver damage protection; All English. Blumea balsamifera chemical constitution and pharmacological research progress [J]. Chinese current clinical medicine magazine; 2006,4 (21): 1941-1945.].
Flavones is one big type of material that occurring in nature extensively exists, and comprises polytypes such as flavones, flavanone, isoflavones, chalcone.The flavones that a lot of plants are contained not is certain single flavone, as: the Radix Astragali contains flavones and isoflavones two flavonoids simultaneously; Contain the tuber of dwarf lilyturf such as flavanones and homoisoflavone two flavonoids etc. simultaneously.Therefore, the notion of general flavone is meant all dissimilar flavone compound sums in the material.Flavonoids chemical constitution in the Blumea balsamifera mainly contains two kinds: flavanone, like blumeatin A (3,3 ', 5,7-tetramethyl-4 '-melonia flavones), Blumea balsamifera element etc.; Flavones is like rutin, Quercetin etc.The key distinction of two flavonoids structures is: in the flavanone between C-2 and the C-3 for singly-bound, and be pair keys between C-2 and the C-3 in the flavones.Because the difference of two flavonoids structures causes its ultraviolet absorpting spectrum also different fully.UV scanning collection of illustrative plates such as the accompanying drawing 1 of representation compound blumeatin A of two flavonoids (3,3 ', 5,7-tetramethyl-4 '-melonia flavones) and rutin.
At present, a lot of bibliographical informations about determination of total flavonoids in Chinese crude drug or the extract are arranged, wherein also comprise Blumea balsamifera [Huang Yonglin; Zhao Zhiguo, civilian Yongxin. content of total flavone is measured [J] in the different parts Blumea balsamifera. GUIHAIA, 2006; 26 (4): 453-455.] and blumea riparia [Jiang Jianping, Du Xiu, Qiu Qin; Lan Renqing, Li Shengmao, Zhen Hanshen. content of total flavone is measured [J] in the blumea riparia of the different places of production. Colleges Of Traditional Chinese Medicine Of Guangxi's journal; 2009,12 (4): 44-46.] content of total flavone assay method in.In the existing method of measuring general flavone content many with rutin or other flavones ingredient (like aurantiin [Li Jiangping; Wan Yunpeng; Wang Jianyong; Ceng Wenhui. the assay method of general flavone content [P]. Chinese patent: 03114240.0,2003-09-17.]) be reference substance, directly or through adding the method for measuring absorbance at maximum absorption wavelength in aluminium salt colour developing back be achieved.In the document, [Huang Yonglin, Zhao Zhiguo such as Huang Yonglin; Literary composition Yongxin. content of total flavone is measured [J] in the different parts Blumea balsamifera. GUIHAIA, 2006,26 (4): 453-455.] measure the general flavone in the Blumea balsamifera; [Jiang Jianping, Du Xiu, Qiu Qin such as Jiang Jianping; Lan Renqing, Li Shengmao, Zhen Hanshen. content of total flavone is measured [J] in the blumea riparia of the different places of production. Colleges Of Traditional Chinese Medicine Of Guangxi's journal; 2009,12 (4): 44-46.] measure the blumea riparia general flavone content and adopt rutin exactly, add aluminium nitrate, sodium nitrite, sodium hydroxide solution colour developing back and measure as reference substance.It is comparatively loaded down with trivial details that but the method step of general flavone is measured in the colour developing back, and only chromogenic reaction just needs 30 minutes; Stability is also relatively poor, and just inconsistent like maximum absorption wavelength in two pieces of documents of above-mentioned mensuration Blumea balsamifera and blumea riparia general flavone, one piece is 500nm, and another piece of writing is 505nm.The method of directly measuring general flavone of not developing the color is comparatively simple, and for containing single flavone or being master's medicinal material or extract with single flavone, this assay method is that error is also less; But; For medicinal material that contains two flavonoids simultaneously or extract (like Blumea balsamifera); Since at the maximum absorption wavelength 254nm of flavones, be the trough of flavanone uv absorption just, same maximum absorption wavelength 289nm at flavanone; Just be again the trough (like accompanying drawing 1) of flavones uv absorption; At this moment, adopting a kind of flavones maximum absorption wavelength directly to measure general flavone gained result will be less than the sample actual content, and makes that measuring the result produces very big error because of the interference that receives another kind of flavones.
Summary of the invention
The purpose of this invention is to provide a kind of method of accurately, fast measuring general flavone content in the identical material of Blumea balsamifera or Herba Blumeae Balsamiferae extract or Blumea balsamifera total flavones.
The technical scheme that the present invention solves the problems of the technologies described above is:
A kind of method of measuring general flavone content in the identical material of Blumea balsamifera or Herba Blumeae Balsamiferae extract or Blumea balsamifera total flavones.This method system is with rutin or blumeatin A (3; 3 ', 5,7-tetramethyl-4 '-melonia flavones) reference substance is mixed with the gradient standard solution; Blumea balsamifera medicinal material or Herba Blumeae Balsamiferae extract are mixed with need testing solution; According to UV-VIS spectrophotometry, directly measure gradient standard solution absorbance, set up typical curve; Measure the need testing solution absorbance again, read need testing solution concentration from typical curve, the extension rate during at last according to obtain solution calculates the general flavone content of Blumea balsamifera medicinal material or extract, and the concrete operations step is following:
(1) preparation of reference substance solution: precision takes by weighing a certain amount of rutin or blumeatin A (3,3 ', 5,7-tetramethyl-4 '-melonia flavones) reference substance, puts in the volumetric flask, with dissolve with methanol and be settled to scale, as reference substance standard mother liquor;
(2) foundation of typical curve: draw the reference substance standard mother liquor of different volumes respectively, put in the volumetric flask, add methanol constant volume, obtain the standard solution of a series of variable concentrations; Getting above-mentioned standard solution respectively, is blank solvent with methyl alcohol, according to UV-VIS spectrophotometry, measures absorbance, is ordinate with the absorbance, and concentration is horizontal ordinate, the drawing standard curve;
(3) preparation of need testing solution and determination method: with Blumea balsamifera medicinal material or its extract, be mixed with need testing solution,, measure absorbance, read concentration, calculate, promptly get from typical curve according to UV-VIS spectrophotometry.
Above-mentioned content of total flavone assay method preferably be:
(1) preparation of reference substance solution: it is an amount of to get rutin or blumeatin A (3,3 ', 5,7-tetramethyl-4 '-melonia flavones), adds methyl alcohol and processes the solution that contains 0.2～0.5mg among the 1ml, promptly gets;
(2) foundation of typical curve: measuring reference substance solution 0ml, 1ml, 2ml, 3ml, 4ml, 5ml, 6ml, 8ml, put respectively in the 50ml measuring bottle, add methanol constant volume to scale, shake up, is blank with first part; According to UV-VIS spectrophotometry, measure absorbance at 260～280nm place, be ordinate with the absorbance, concentration is horizontal ordinate, the drawing standard curve;
(3) need testing solution preparation and determination method: get Herba Blumeae Balsamiferae extract, porphyrize is got 5～30mg approximately, puts in the 25ml measuring bottle; Add dissolve with methanol and be diluted to scale, shake up, filter, precision is measured subsequent filtrate 2ml; Put in the 50ml measuring bottle, add methanol constant volume, shake up, get need testing solution to scale; According to UV-VIS spectrophotometry, measure absorbance at 260～280nm place, read concentration from typical curve, calculate, promptly get.
Above-mentioned content of total flavone assay method preferably be:
(1) preparation of reference substance solution: it is an amount of that precision takes by weighing blumeatin A (3,3 ', 5,7-tetramethyl-4 '-melonia flavones), adds methyl alcohol and process the solution that contains 0.4mg among the 1ml, promptly gets;
(2) foundation of typical curve: precision is measured reference substance solution 0ml, 1ml, 2ml, 3ml, 4ml, 5ml, 6ml, 8ml, puts respectively in the 50ml measuring bottle, adds methanol constant volume to scale, shake up, and be blank with first part; According to UV-VIS spectrophotometry, measure absorbance at the 271nm place, be ordinate with the absorbance, concentration is horizontal ordinate, the drawing standard curve;
(3) need testing solution preparation and determination method: get Herba Blumeae Balsamiferae extract, porphyrize, precision takes by weighing 20mg, puts in the 25ml measuring bottle; Add dissolve with methanol and be diluted to scale, shake up, filter, precision is measured subsequent filtrate 2ml; Put in the 50ml measuring bottle, add methanol constant volume, shake up, get need testing solution to scale; According to UV-VIS spectrophotometry, measure absorbance at the 271nm place, read concentration from typical curve, calculate, promptly get.
[0019] result is measured in (4): the Blumea balsamifera medicinal substances extract is pressed dry product and is calculated, and contains general flavone with blumeatin A (3,3 ', 5,7-tetramethyl-4 '-melonia flavones) meter, must not be less than 55.0%.
Research and explanation to the said determination method:
1, the investigation of condition determination:
(1) instrument and reagent: ultraviolet spectrophotometer, Herba Blumeae Balsamiferae extract, blumeatin A (3,3 ', 5,7-tetramethyl-4 '-melonia flavones) reference substance (purity 99.8%) is analyzed pure methyl alcohol.
(2) selection of assay method: according to the chemical constitution study result, adopt the isoabsorptive point wavelength of rutin and blumeatin A (3,3 ', 5,7-tetramethyl-4 '-melonia flavones), measure content of total flavone in the Herba Blumeae Balsamiferae extract.
(3) selection of reference substance: select the reference substance of blumeatin A (3,3 ', 5,7-tetramethyl-4 '-melonia flavones) as these article assay.
(4) test sample preparation method and assay method: after deliberation, confirm that test sample preparation and assay method are: get Herba Blumeae Balsamiferae extract, porphyrize, precision takes by weighing 20mg; Put in the 25ml measuring bottle, add dissolve with methanol and be diluted to scale, shake up; Filter, precision is measured subsequent filtrate 2ml, puts in the 50ml measuring bottle; Add methanol constant volume to scale, shake up, get need testing solution; According to UV-VIS spectrophotometry, measure absorbance at the 271nm place, read concentration from typical curve, calculate, promptly get.
2, blumeatin A (3,3 ', 5,7-tetramethyl-4 '-melonia flavones) linear relationship
(1) preparation of reference substance solution: it is an amount of that precision takes by weighing blumeatin A (3,3 ', 5,7-tetramethyl-4 '-melonia flavones), adds methyl alcohol and process the solution that contains 0.4mg among the 1ml, promptly gets.
(2) foundation of typical curve: precision is measured reference substance solution 0ml, 1ml, 2ml, 3ml, 4ml, 5ml, 6ml, 8ml, puts respectively in the 50ml measuring bottle, adds methanol constant volume to scale, shake up, and be blank with first part; According to UV-VIS spectrophotometry, measure absorbance at the 271nm place, be ordinate with the absorbance, concentration is horizontal ordinate, the drawing standard curve; Measure the result and see table 1, typical curve is seen accompanying drawing 2.
Regression equation: y=24.293x-0.0005
Wherein x is concentration (μ g/ml), and y is an absorbance log
Related coefficient: r=1, the range of linearity is: 0～64.896 μ g/ml
Table 1. blumeatin A (3,3 ', 5,7-tetramethyl-4 '-melonia flavones) typical curve
3, precision test
Get with a blumeatin A (3,3 ', 5,7-tetramethyl-4 '-melonia flavones) reference substance solution,, measure absorbance at the 271nm place according to UV-VIS spectrophotometry, METHOD FOR CONTINUOUS DETERMINATION 6 times, the result sees table 2, the result shows that instrument precision is good.
Table 2. instrument precision is investigated data
4, stability test
Get Herba Blumeae Balsamiferae extract, process need testing solution by above-mentioned need testing solution preparation method, measure its absorbance log at different time respectively, the result sees table 3, and the result shows that sample is stable in 24hr.
Table 3. sample stability is investigated data
5, replica test
Get with 6 parts of a collection of Herba Blumeae Balsamiferae extracts, measure by above-mentioned content assaying method, the result sees table 4, and the result shows sample determination method favorable reproducibility.
Table 4. repeatability is investigated data
6, application of sample recovery test
Get with 9 parts of a collection of Herba Blumeae Balsamiferae extracts (content is 79.0%), the accurate title, decide, and puts in the measuring bottle; Press 80%, 100%, 120% adding blumeatin A (3,3 ', 5 of general flavone content in the extract respectively; 7-tetramethyl-4 '-melonia flavones) reference substance; Measure by above-mentioned content assaying method, calculate content of total flavone and average recovery, the result sees table 5.
Table 5. application of sample reclaims investigates data
7, sample size is measured
Get 6 batches of Herba Blumeae Balsamiferae extracts, measure by above-mentioned content assaying method, calculate content of total flavone, the result sees table 5.
Table 6. sample size is measured the result
Measure the result per sample, regulation: these article are pressed dry product and are calculated, and contain general flavone with blumeatin A (3,3 ', 5,7-tetramethyl-4 '-melonia flavones) meter, must not be less than 55.0%.
Beneficial effect of the present invention:
1, the invention provides assay method to general flavone content in the identical material of Blumea balsamifera or Blumea balsamifera total flavones.Contain two flavonoids in the Blumea balsamifera: flavanone (represent composition: blumeatin A) and flavones (represent composition: rutin); Through research; Find that two flavonoids ultraviolet light absorptions have very big-difference, but near the 271nm wavelength, two flavonoids has isoabsorptive point (seeing accompanying drawing 1).The most accurate through the rutin of distinct methods mensuration known content and the result of blumeatin A reference substance mixed solution being compared (seeing table 7), prove the result who adopts isoabsorptive point wavelength mensuration to contain general flavone content in flavanone and the flavones ingredient material simultaneously.Therefore, the present invention can be used for measuring content of total flavone in the Blumea balsamifera, thereby more effectively controls the quality of Blumea balsamifera or Herba Blumeae Balsamiferae extract or Blumea balsamifera total flavones same substance.
The different determination of total flavonoids methods of table 7. relatively
Annotate:
1.Adopt the inventive method to measure the result;
2.Adopt the rutin maximum absorption wavelength directly to measure the result;
3.Adopt the blumeatin A maximum absorption wavelength directly to measure the result;
4.Adopt rutin colour developing back maximum absorption wavelength mensuration result in the document.
2, the positive defending party to the application of the present invention is used for raw material quality control, measures like Blumea balsamifera medicinal material content of total flavone; Semi-manufactured goods quality control is measured like content of total flavone in the Herba Blumeae Balsamiferae extract; And end product quality control, measure like content of total flavone in the Blumea balsamifera Chinese patent medicine preparation.
Owing to adopt the isoabsorptive point wavelength of flavones and flavanone directly to measure the general flavone content in the Herba Blumeae Balsamiferae extract, solved the inconsistent determination of total flavonoids difficulty of bringing of two flavonoids uv absorption effectively.Through retrieval, do not find to have and adopt isoabsorptive point to measure the relevant report that contains the general flavone content of dissimilar flavones in the material, also not finding has the relevant report that adopts isoabsorptive point to measure other dissimilar composition total contents.
Description of drawings
Fig. 1 is the UV scanning figure of close concentration blumeatin A and rutin.
Among the figure: dotted line is rutin (40.76 μ g/ml) UV scanning figure, and solid line is blumeatin A (40.56 μ g/ml) UV scanning figure.
Blumeatin A typical curve in Fig. 2 determination of total flavonoids.
Embodiment
Below in conjunction with instance the present invention is further specified, following instance only is used to the present invention is described but not limitation of the present invention.
Instance 1: content of total flavone assay method in the Herba Blumeae Balsamiferae extract:
(1) preparation of reference substance solution: it is an amount of that precision takes by weighing blumeatin A (3,3 ', 5,7-tetramethyl-4 '-melonia flavones), adds methyl alcohol and process the solution that contains 0.4mg among the 1ml, promptly gets;
(2) foundation of typical curve: precision is measured reference substance solution 0ml, 1ml, 2ml, 3ml, 4ml, 5ml, 6ml, 8ml, puts respectively in the 50ml measuring bottle, adds methanol constant volume to scale, shake up, and be blank with first part; According to UV-VIS spectrophotometry, measure absorbance at the 271nm place, be ordinate with the absorbance, concentration is horizontal ordinate, the drawing standard curve;
(3) need testing solution preparation and determination method: get lot number and be 20101022 Herba Blumeae Balsamiferae extract, porphyrize, precision takes by weighing 19.58mg; Put in the 25ml measuring bottle, add dissolve with methanol and be diluted to scale, shake up; Filter, precision is measured subsequent filtrate 2ml, puts in the 50ml measuring bottle; Add methanol constant volume to scale, shake up, get need testing solution; According to UV-VIS spectrophotometry, measure absorbance at the 271nm place, read concentration from typical curve, calculate, promptly get.
(4) measure the result: the Blumea balsamifera medicinal substances extract is pressed dry product and is calculated, and contains general flavone with blumeatin A (3,3 ', 5,7-tetramethyl-4 '-melonia flavones) meter, is 79.0%.
Instance 2: content of total flavone assay method in the Chinese mugwort heart ketone sheet (Blumea balsamifera total flavones preparation):
(1) preparation of reference substance solution: it is an amount of that precision takes by weighing blumeatin A (3,3 ', 5,7-tetramethyl-4 '-melonia flavones), adds methyl alcohol and process the solution that contains 0.4mg among the 1ml, promptly gets;
(2) foundation of typical curve: precision is measured reference substance solution 0ml, 1ml, 2ml, 3ml, 4ml, 5ml, 6ml, 8ml, puts respectively in the 50ml measuring bottle, adds methanol constant volume to scale, shake up, and be blank with first part; According to UV-VIS spectrophotometry, measure absorbance at the 271nm place, be ordinate with the absorbance, concentration is horizontal ordinate, the drawing standard curve;
(3) need testing solution preparation and determination method: get lot number and be 20 of 20101029 Chinese mugwort heart ketone sheets, accurate claim fixed, porphyrize, precision takes by weighing in right amount (being equivalent to blumea balsamifera flavone extract 20mg approximately); Put in the 25ml measuring bottle, add dissolve with methanol and be diluted to scale, shake up; Filter, precision is measured subsequent filtrate 2ml, puts in the 50ml measuring bottle; Add methanol constant volume to scale, shake up, get need testing solution; According to UV-VIS spectrophotometry, measure absorbance at the 271nm place, read concentration from typical curve, calculate, promptly get.
(4) measure the result: Chinese mugwort heart ketone sheet is pressed dry product and is calculated, and contains general flavone with blumeatin A (3,3 ', 5,7-tetramethyl-4 '-melonia flavones) meter, is 17.2%.
Instance 3: content of total flavone assay method in the Blumea balsamifera medicinal material:
(1) preparation of reference substance solution: it is an amount of that precision takes by weighing blumeatin A (3,3 ', 5,7-tetramethyl-4 '-melonia flavones), adds methyl alcohol and process the solution that contains 0.4mg among the 1ml, promptly gets;
(2) foundation of typical curve: precision is measured reference substance solution 0ml, 1ml, 2ml, 3ml, 4ml, 5ml, 6ml, 8ml, puts respectively in the 50ml measuring bottle, adds methanol constant volume to scale, shake up, and be blank with first part; According to UV-VIS spectrophotometry, measure absorbance at the 271nm place, be ordinate with the absorbance, concentration is horizontal ordinate, the drawing standard curve;
(3) need testing solution preparation and determination method: it is 20091015 Blumea balsamifera medicinal powder 0.25g that precision takes by weighing lot number, puts in the cable type extractor according, and the medicine residue is dried in the extracting 1 hour that adds diethyl ether; Put in the round-bottomed flask, add methyl alcohol 50ml and refluxed 1 hour, put cold; Filter, precision is measured subsequent filtrate 1ml, puts in the 25ml measuring bottle; Add methanol constant volume to scale, shake up, get need testing solution; According to UV-VIS spectrophotometry, measure absorbance at the 271nm place, read concentration from typical curve, calculate, promptly get.
(4) measure the result: the Blumea balsamifera medicinal material is pressed dry product and is calculated, and contains general flavone with blumeatin A (3,3 ', 5,7-tetramethyl-4 '-melonia flavones) meter, is 8.1%.
Claims (3)
1. a Blumea balsamifera total flavones Determination on content method is a kind of method of measuring general flavone content in the identical material of Blumea balsamifera or Herba Blumeae Balsamiferae extract or Blumea balsamifera total flavones; This method system is mixed with the gradient standard solution with rutin or blumeatin A reference substance, and Blumea balsamifera medicinal material or Herba Blumeae Balsamiferae extract are mixed with need testing solution, according to UV-VIS spectrophotometry, directly measure gradient standard solution absorbance, set up typical curve; Measure the need testing solution absorbance again, read need testing solution concentration from typical curve, the extension rate during at last according to obtain solution calculates the general flavone content of Blumea balsamifera medicinal material or extract, and the concrete operations step is following:
(1) preparation of reference substance solution: precision takes by weighing a certain amount of rutin or blumeatin A reference substance, in dissolve with methanol and constant volume and volumetric flask, as reference substance standard mother liquor;
(2) foundation of typical curve: draw the reference substance standard mother liquor of different volumes respectively, put in the volumetric flask, add methanol constant volume, obtain the standard solution of a series of variable concentrations; Getting above-mentioned standard solution respectively, is blank solvent with methyl alcohol, according to UV-VIS spectrophotometry, measures absorbance, is ordinate with the absorbance, and concentration is horizontal ordinate, the drawing standard curve;
(3) preparation of need testing solution and determination method: with Blumea balsamifera medicinal material or its extract, be mixed with need testing solution,, measure absorbance, read concentration, calculate, promptly get from typical curve according to UV-VIS spectrophotometry.
2. the content assaying method of Blumea balsamifera total flavones according to claim 1 is characterized in that:
The preparation of described step (1) reference substance solution: get rutin or blumeatin A is an amount of, add methyl alcohol and process the solution that contains 0.2～0.5mg among the 1ml, promptly get;
The foundation of described step (2) typical curve: measuring reference substance solution 0ml, 1ml, 2ml, 3ml, 4ml, 5ml, 6ml, 8ml, put respectively in the 50ml measuring bottle, add methanol constant volume to scale, shake up, is blank with first part; According to UV-VIS spectrophotometry, measure absorbance at 260～280nm place, be ordinate with the absorbance, concentration is horizontal ordinate, the drawing standard curve;
Preparation of described step (3) need testing solution and determination method: get Herba Blumeae Balsamiferae extract, porphyrize is got 5～30mg approximately; Put in the 25ml measuring bottle, add dissolve with methanol and be diluted to scale, shake up; Filter, precision is measured subsequent filtrate 2ml, puts in the 50ml measuring bottle; Add methanol constant volume to scale, shake up, get need testing solution; According to UV-VIS spectrophotometry, measure absorbance at 260～280nm place, read concentration from typical curve, calculate, promptly get.
3. like the content assaying method of the said Blumea balsamifera total flavones of claim 2, it is characterized in that:
The preparation of described step (1) reference substance solution: it is an amount of that precision takes by weighing blumeatin A, adds methyl alcohol and process the solution that contains 0.4mg among the 1ml, promptly gets;
The foundation of described step (2) typical curve: precision is measured reference substance solution 0ml, 1ml, 2ml, 3ml, 4ml, 5ml, 6ml, 8ml, puts respectively in the 50ml measuring bottle, adds methanol constant volume to scale, shakes up, and be blank with first part; According to UV-VIS spectrophotometry, measure absorbance at the 271nm place, be ordinate with the absorbance, concentration is horizontal ordinate, the drawing standard curve;
Preparation of described step (3) need testing solution and determination method: get Herba Blumeae Balsamiferae extract, porphyrize, precision takes by weighing 20mg; Put in the 25ml measuring bottle, add dissolve with methanol and be diluted to scale, shake up; Filter, precision is measured subsequent filtrate 2ml, puts in the 50ml measuring bottle; Add methanol constant volume to scale, shake up, get need testing solution; According to UV-VIS spectrophotometry, measure absorbance at the 271nm place, read concentration from typical curve, calculate, promptly get.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109142257A (en) * | 2017-06-28 | 2019-01-04 | 贵州艾源生态药业开发有限公司 | A kind of measuring method of Blumea balsamifera total flavones content |
| CN107515259A (en) * | 2017-08-03 | 2017-12-26 | 深圳海王医药科技研究院有限公司 | The assay method of blumeatin A content in Chinese mugwort heart ketone piece |
| CN109856264A (en) * | 2019-01-11 | 2019-06-07 | 遵义医学院 | The detection method of active constituent content in Blumea balsamifera medicinal material |
| CN109856264B (en) * | 2019-01-11 | 2021-09-07 | 遵义医科大学 | Method for detecting content of effective components in blumea balsamifera medicinal material |
| CN110542664A (en) * | 2019-09-09 | 2019-12-06 | 湖北省农业科学院中药材研究所 | Method for detecting content of total flavonoids in vine tea |
| CN111122487A (en) * | 2020-01-10 | 2020-05-08 | 贵州中医药大学 | Method for measuring content of quercetin in coriaria sinica maxim medicinal material |
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