CN114324568A - Sound field auxiliary preparation method of guanine peptide nucleic acid self-assembly nanosphere-based photonic crystal - Google Patents
Sound field auxiliary preparation method of guanine peptide nucleic acid self-assembly nanosphere-based photonic crystal Download PDFInfo
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Abstract
The invention discloses a sound field auxiliary preparation method of guanine peptide nucleic acid self-assembly nanosphere-based photonic crystals. Taking guanine peptide nucleic acid self-assembly nanospheres as a light reflection functional medium material, forming photonic crystals by adopting a compact hexagonal packing structure, and rapidly forming the photonic crystals by combining sound field control and micro-fluidic control; the morphology and the particle size of the self-assembled nanospheres are regulated and controlled by regulating the influence factors of self-assembly and the nucleic acid molecular structure of the guanine peptide, the arrangement is regulated and controlled by regulating and controlling sound field parameters, and finally the wavelength of the reflected light of the photonic crystal is regulated and controlled. The invention realizes the preparation of guanine peptide nucleic acid self-assembly nanosphere-based photonic crystals by utilizing a sound field, has good biocompatibility and excellent optical performance and dynamic response characteristic, and the photonic crystals can change lattice parameters under external excitation so as to realize the translation of reflected light wavelength and the change of visual color.
Description
Technical Field
The invention relates to a preparation method of a biological organic photonic crystal, in particular to sound field assisted preparation of a guanine peptide nucleic acid self-assembled nano sphere-based photonic crystal.
Background
The photonic crystal is orderly and regularly and periodically stacked through media with different dielectric constants, so that selective reflection of incident light can be realized. Compared with the traditional dye and fluorescent substance, the photonic crystal has the characteristics of adjustable reflected light, difficult quenching of the reflected light and the like, can realize the functions of optical anti-counterfeiting, optical information storage, signal transmission and the like, and has wide application prospect in the fields of biomedical sensing, intelligent tattooing, man-machine interaction, micro-strain detection, colorimetric sensing, photonic chips and the like.
Most of the existing photonic crystals adopt inorganic oxides (such as silicon dioxide, titanium dioxide and the like) and high molecular polymers (such as polystyrene, polyurethane and the like) as functional medium materials; the preparation process usually adopts laser direct writing etching, three-dimensional printing, template printing, colloid self-assembly and the like. However, although the photonic crystal prepared from the polymer photonic crystal and the inorganic oxide has higher physical and chemical stability and narrower reflection bandwidth, the photonic crystal can be applied to the fields of optical information expression, optical anti-counterfeiting and the like; however, with the development of photonic probes and biomedical sensing, photonic crystals with good biocompatibility will become a necessary trend in the development of this field. The weak biocompatibility causes the biological rejection reaction of the traditional photonic crystal, the shape of the array is not easy to control, the preparation period is long, the preparation process is complex, and the development requirement of the new generation of biocompatible photonic crystal is difficult to adapt.
Disclosure of Invention
Based on the technical background, the invention provides a sound field-assisted preparation method of a guanine peptide nucleic acid self-assembled nano sphere-based photonic crystal by combining the characteristics of inherent biocompatibility of a basic group and a peptide molecule, easiness in regulation and control of the appearance of a self-assembled body and capability of regulating and controlling the appearance of an array under the assistance of a sound field.
The invention realizes the preparation of the guanine peptide nucleic acid self-assembly nano sphere-based photonic crystal by using the sound field, and has excellent optical performance and dynamic response characteristic while having good biocompatibility.
The technical scheme adopted by the invention for solving the technical problems is as follows:
the method is characterized in that the guanine peptide nucleic acid self-assembly nanospheres are prepared by adopting a bulk acoustic wave sound field auxiliary mode, and the guanine peptide nucleic acid self-assembly nanospheres are formed by self-assembling guanine peptide nucleic acid.
The method adopts a bulk acoustic wave transducer to form a bulk acoustic wave sound field auxiliary mode.
The guanine peptide nucleic acid self-assembly nanosphere-based photonic crystal is encapsulated by polyethylene glycol diacrylate (PEGDA200) with the average molecular weight of 200.
The preparation method specifically comprises the following steps:
1) dissolving guanine peptide nucleic acid powder in ultrapure water, standing at a constant temperature of 25 ℃ and a constant humidity of 80% for 36 hours to prepare guanine peptide nucleic acid self-assembly nanosphere suspension;
2) the substrate is arranged at the bottom in the bulk acoustic wave transducer in advance, turbid liquid is pumped into a cavity of the bulk acoustic wave transducer, and a signal generator of the bulk acoustic wave transducer is turned on to adjust excitation frequency to form a stable and consistent standing wave field in the cavity;
3) standing until the guanine peptide nucleic acid self-assembly nanospheres form stable arrangement in a bulk acoustic standing wave field, and then pumping out redundant turbid liquid in the chamber to slowly deposit the guanine peptide nucleic acid self-assembly nanospheres on the surface of the substrate to prepare the substrate attached with the guanine peptide nucleic acid self-assembly nanosphere-based photonic crystals, so as to obtain the guanine peptide nucleic acid self-assembly nanosphere-based photonic crystals.
The invention takes the guanine peptide nucleic acid self-assembly nanosphere as a functional medium material, so that the photonic crystal product has inherent biocompatibility and other specified biological activity functions on the basis of having excellent optical characteristics.
The guanine peptide nucleic acid self-assembly nano sphere-based photonic crystal adopts a glass sheet, a quartz sheet, a silicon wafer or a flexible substrate as a substrate, and the flexible substrate is a polydimethylsiloxane film and the like.
The morphology regulation (particle size, hollow/solid, smooth or rough surface and the like) of the guanine peptide nucleic acid self-assembly nanosphere is realized by regulating the molecular structure of the guanine peptide nucleic acid.
In particular, the amount of the solvent to be used,
the hollow/solid degree in the self-assembly nanosphere of the guanine peptide nucleic acid is adjusted by adjusting the length and the variety of hydrophilic and hydrophobic groups in the molecular structure of the guanine peptide nucleic acid;
the surface smoothness/roughness of the self-assembly nanosphere of the guanine peptide nucleic acid is adjusted by adjusting the number and the types of hydrophilic and hydrophobic groups in the molecular structure of the guanine peptide nucleic acid.
The particle size of the self-assembly nano sphere of the guanine peptide nucleic acid is regulated and controlled by adjusting the temperature,
the arrangement regulation and control of the guanine peptide nucleic acid self-assembly nanosphere-based photonic crystals are realized by regulating and controlling sound field parameters;
the regulation and control of the wavelength of the light reflected by the guanine peptide nucleic acid self-assembled nano sphere-based photonic crystal are realized by combining particle size regulation and arrangement regulation and control.
The guanine peptide nucleic acid is an amino acid-like structure with guanine as a side chain group and an amido bond as a molecular skeleton, and the groups for artificially synthesizing the guanine peptide nucleic acid are diphenyl methoxycarbonyl (Bhoc), 9-fluorenylmethoxycarbonyl (Fmoc), glycine, aminoethyl, acetyl and guanine groups.
The specific molecular formula of the guanine peptide nucleic acid is Bhoc-G- (Bhoc) -aeg-OH, Bhoc-G, Fmoc-G, Fmoc-G- (Fmoc) -aeg-OH, G- (Bhoc) -aeg-OH, Fmoc-G-aeg-OH and Fmoc-G- (Bhoc) -aeg-OH.
The guanine peptide nucleic acid self-assembly nanospheres are arranged in the bulk acoustic wave transducer in an auxiliary mode through a bulk acoustic wave standing wave field, the shape formed by arrangement is a one-dimensional stripe shape, a two-dimensional lattice shape or a two-dimensional curve shape, and the two-dimensional curve shape is specifically a wave shape.
The bulk acoustic wave transducer forming two-dimensional lattice shape arrangement mainly comprises a three-dimensional printing chamber, two pairs of piezoelectric ceramics which are vertically distributed and a micro-channel controlled by an injection pump.
The bulk acoustic wave transducer forming one-dimensional stripe shape arrangement mainly comprises a three-dimensional printing chamber, a pair of piezoelectric ceramics which are vertically distributed and a micro-channel controlled by an injection pump.
The bulk acoustic wave transducer forms a frequency-adjustable bulk acoustic wave standing wave field in the guanine peptide nucleic acid self-assembly nanosphere turbid liquid.
The guanine peptide nucleic acid self-assembly nanosphere-based photonic crystal is arranged by using a bulk acoustic wave sound field in an auxiliary mode, the lattice distance is more than or equal to 20 micrometers, and the stripe array distance is more than or equal to 20 micrometers.
The guanine peptide nucleic acid self-assembly nanosphere-based photonic crystal is further processed by adopting the following process to complete packaging preparation:
1) weighing 5 ml of polyethylene glycol diacrylate with the average molecular weight of 200, and adding a mixed photoinitiator TPO-L accounting for 0.8% of the mass fraction of the polyethylene glycol diacrylate to form a mixture;
2) fully mixing and defoaming the mixture by using a planetary stirrer to prepare and form a photosensitive prepolymer;
3) placing the substrate attached with the guanine peptide nucleic acid self-assembly nano sphere-based photonic crystal on a spin coating instrument, uniformly dripping a photosensitive prepolymer on the surface of the guanine peptide nucleic acid self-assembly nano sphere-based photonic crystal without the substrate, forming a uniform thin film on the surface of the guanine peptide nucleic acid self-assembly nano sphere-based photonic crystal by using a spin coating method, and curing by using ultraviolet light to finish the packaging of the guanine peptide nucleic acid self-assembly nano sphere-based photonic crystal.
In the step 3), ultraviolet light with the wavelength of 405 nanometers is used for curing for 20 seconds.
The guanine peptide nucleic acid self-assembly nanosphere-based photonic crystal is packaged, the height is less than 5 mm, and the diameter is more than 20 mm.
The guanine peptide nucleic acid self-assembly nano sphere-based photonic crystal is prepared by processing guanine peptide nucleic acid self-assembly nano spheres in a bulk acoustic wave sound field auxiliary mode.
The guanine peptide nucleic acid self-assembly nanosphere is used as a light reflection functional medium material, a compact hexagonal packing structure can be adopted to form photonic crystals, a silicon dioxide sheet, a polydimethylsiloxane film, a quartz sheet and the like are used as substrates, polyethylene glycol diacrylate is used for packaging, and a method combining sound field control and micro-fluidic control is adopted to carry out rapid forming on the photonic crystals. The morphology and the particle size of the guanine peptide nucleic acid self-assembly nanospheres are regulated and controlled by regulating the influence factors such as self-assembly environmental conditions, molecular structures and the like, and the arrangement regulation and control of the guanine peptide nucleic acid self-assembly nanosphere-based photonic crystals are realized by regulating and controlling sound field parameters; thereby realizing the regulation and control of the wavelength of the light reflected by the guanine peptide nucleic acid self-assembled nano sphere-based photonic crystal.
The photonic crystal can change lattice parameters under external excitation (such as temperature, bending, stretching, magnetic field, electric field, pH and humidity) so as to realize translation of reflected light wavelength and change of visual color.
The photonic crystal adopts guanine peptide nucleic acid self-assembly nanospheres as a functional medium material, utilizes the diffraction principle of light, regularly and orderly stacks the guanine peptide nucleic acid self-assembly nanospheres in one dimension or two dimensions to form the guanine peptide nucleic acid self-assembly nanosphere-based photonic crystal capable of selectively reflecting incident light at different angles, and simultaneously uses a sound field auxiliary means to prepare the guanine peptide nucleic acid self-assembly nanosphere-based photonic crystal.
The preparation method adopts a sound field auxiliary means to prepare the guanine peptide nucleic acid self-assembly nanosphere-based photonic crystal, and can realize the regulation and control of the morphology of the guanine peptide nucleic acid self-assembly nanosphere array by regulating the hydrophilicity and hydrophobicity of the photonic crystal substrate, the sound field frequency and the voltage amplitude as well as the microfluidic pumping speed.
The guanine peptide nucleic acid self-assembly nanosphere-based photonic crystal can regulate and control the particle size and the appearance of the nanosphere by using a method of molecular modification and self-assembly condition control, thereby realizing the regulation and control of the optical characteristics of the guanine peptide nucleic acid nanosphere-based photonic crystal.
The invention has the beneficial effects that:
(1) the morphology (hollow/solid, smooth or rough surface and the like) of the self-assembled nano sphere of the guanine peptide nucleic acid is regulated and controlled by carrying out design modification on the molecular structure of the guanine peptide nucleic acid;
(2) the particle size of the guanine peptide nucleic acid self-assembly nanospheres in the guanine peptide nucleic acid self-assembly nanosphere-based photonic crystal is regulated and controlled by controlling the self-assembly conditions (such as temperature and the like), so that the optical performance of the photonic crystal is regulated and controlled;
(3) the guanine peptide nucleic acid self-assembly nanospheres are orderly stacked and arranged, so that the selective reflection of incident light is realized, and meanwhile, good biocompatibility is kept;
(4) the shape structure of the guanine peptide nucleic acid self-assembled nano-sphere-based photonic crystal can be regulated and controlled by adjusting the hydrophilicity and hydrophobicity, the sound field frequency and the voltage amplitude of the base of the guanine peptide nucleic acid self-assembled nano-sphere-based photonic crystal and the microfluidic pumping speed.
Drawings
FIG. 1 is a schematic diagram of a process for preparing a guanine peptide nucleic acid self-assembly nanosphere suspension;
FIG. 2 is scanning electron microscope image of surface rough solid guanine peptide nucleic acid self-assembled nanospheres;
FIG. 3 is scanning electron microscope image of self-assembled nanospheres of guanine peptide nucleic acid with smooth and solid surfaces;
FIG. 4 is scanning electron microscope image of surface smooth hollow guanine peptide nucleic acid self-assembled nanospheres;
FIG. 5 is a schematic diagram of a guanine peptide nucleic acid self-assembled nanosphere structure;
FIG. 6 is a statistical graph of the particle size of self-assembled guanine peptide nucleic acid nanospheres prepared at 4 degrees Celsius;
FIG. 7 is a statistical graph of the particle size of guanine peptide nucleic acid self-assembled nanospheres prepared at 25 deg.C;
FIG. 8 is a statistical graph of the particle size of self-assembled guanine peptide nucleic acid nanospheres prepared at 75 degrees Celsius;
FIG. 9 is a confocal microscope of the stripe arrangement of guanine peptide nucleic acid self-assembled nanosphere-based photonic crystals at different pumping speeds;
FIG. 10 is a diagram of guanine peptide nucleic acid nanosphere-based photonic crystals prepared at different self-assembly temperatures;
FIG. 11 is a graph of the transmission light spectrum of guanine peptide nucleic acid self-assembled nanosphere-based photonic crystals;
FIG. 12 is a graph of guanine peptide nucleic acid self-assembled nano-sphere based photonic crystal transmission peak-photonic crystal bend angle variation.
Detailed Description
The present invention will be further described with reference to the following drawings and examples, but the embodiments of the present invention are not limited thereto.
The embodiment of the invention and the implementation process thereof are as follows:
detailed description of the preferred embodiment 1
Respectively taking 4 mg of guanine peptide nucleic acid in a beaker, adding ultrapure water according to the proportion of 2 mg/ml, heating to over 90 ℃ by using a water bath, and preparing a guanine peptide nucleic acid solution by matching with magnetic stirring. And standing the prepared solution at 25 ℃ and 80% constant humidity for 36 hours to ensure that the guanine peptide nucleic acid is completely self-assembled. The self-assembly nanosphere structure of guanine peptide nucleic acid with rough surface and solid surface is obtained by self-assembly of guanine peptide nucleic acid and shown in figure 2, the self-assembly nanosphere structure of guanine peptide nucleic acid with smooth surface and solid surface is obtained by self-assembly and shown in figure 3, and the self-assembly nanosphere of guanine peptide nucleic acid with smooth surface and hollow core is obtained by self-assembly and shown in figure 4.
It can be seen from this example that the particle size and surface morphology (such as hollow/solid, smooth or rough surface, etc.) of the guanine peptide nucleic acid self-assembled nanosphere can be controlled by adjusting the number and species of hydrophilic and hydrophobic groups in the guanine peptide nucleic acid molecular structure.
Specific example 2
As shown in FIG. 1, 12 mg of guanine peptide nucleic acid (Fmoc-G- (Bhoc) -aeg-OH) was put into a beaker, ultrapure water was added at a ratio of 2 mg/ml, and the resulting mixture was heated to 90 ℃ or higher in a water bath, followed by magnetic stirring to prepare a guanine peptide nucleic acid solution. And trisecting the prepared solution, and standing at 80% constant humidity at 4 deg.C, 25 deg.C and 75 deg.C for 36 hr to completely self-assemble guanine peptide nucleic acid to obtain hollow guanine peptide nucleic acid self-assembled nanospheres with different particle sizes. The morphology of the guanine peptide nucleic acid self-assembly nanosphere is shown in fig. 4, the structural schematic diagram of the guanine peptide nucleic acid self-assembly nanosphere is shown in fig. 5, and the particle size statistical result of the guanine peptide nucleic acid self-assembly nanosphere is shown in fig. 6-8.
As can be seen from the present example, the particle size of the self-assembly nanosphere of guanine peptide nucleic acid can be adjusted by adjusting the self-assembly environment (e.g., temperature, etc.) of guanine peptide nucleic acid.
Specific example 3
(1) As shown in FIG. 1, 12 mg of guanine peptide nucleic acid (Fmoc-G- (Bhoc) -aeg-OH) was put into a beaker, ultrapure water was added at a ratio of 2 mg/ml, and the resulting mixture was heated to 90 ℃ or higher using a water bath, and then a guanine peptide nucleic acid solution was prepared by magnetic stirring. And standing the prepared solution at 25 ℃ and 80% constant humidity for 36 hours to completely self-assemble the guanine peptide nucleic acid to obtain the guanine peptide nucleic acid self-assembled nanosphere suspension.
(2) The one-dimensional and two-dimensional bulk acoustic wave transducers comprise a three-dimensional printing chamber, piezoelectric ceramics and a micro-channel part. Taking a photonic crystal in which guanine peptide nucleic acid nanospheres prepared by self-assembly at 25 ℃ are arranged in one dimension as an example, a three-dimensional printing chamber pasted with piezoelectric ceramics is arranged on a substrate, and a prepared guanine peptide nucleic acid self-assembly nanosphere suspension is pumped into an open chamber from a suspension inflow port through a micro-channel pipeline by using an injection pump.
(3) And after the self-assembly nanosphere suspension is completely pumped into the chamber, starting the signal generator and outputting an electric signal with the frequency of f1, wherein the piezoelectric ceramic generates a bulk acoustic wave standing wave field in the chamber. The guanine peptide nucleic acid self-assembly nanosphere turbid liquid is subjected to the acoustic radiation force with controllable intensity when flowing through the bulk acoustic standing wave field, moves for different distances in the width direction of the cavity and is arranged at the node position of the bulk acoustic standing wave field. After the guanine peptide nucleic acid self-assembly nanospheres are completely arranged, an injection pump is used for pumping redundant liquid in the chamber along the outflow port of the suspension at the speed of 10-100 microliters/minute, so that the guanine peptide nucleic acid self-assembly nanospheres can be uniformly deposited on the surface layer of the substrate, and then the body acoustic wave transducer is removed, and the one-dimensionally arranged striped guanine peptide nucleic acid self-assembly nano-based photonic crystal is formed. The morphology of the guanine peptide nucleic acid self-assembled nanosphere-based photonic crystal under different pumping speeds is shown in fig. 9.
(4) 5 ml of polyethylene glycol diacrylate (PEGDA200) with the average molecular mass of 200 is taken, and a photoinitiator (TPO-L) accounting for 0.8 percent of the mass fraction of the polyethylene glycol diacrylate is added to prepare the photosensitive prepolymer for the guanine peptide nucleic acid self-assembly nano sphere-based photonic crystal encapsulation.
(5) And uniformly coating the photosensitive prepolymer on the surface layer of the guanine peptide nucleic acid self-assembly nano sphere-based photonic crystal by using a spin coating method, and then curing the photosensitive prepolymer by using 405 nano ultraviolet light to finish the packaging of the guanine peptide nucleic acid nano sphere-based photonic crystal.
A diagram of guanine peptide nucleic acid self-assembled nanosphere-based photonic crystals prepared from guanine peptide nucleic acid nanospheres with different particle sizes is shown in FIG. 10.
(6) And performing optical performance characterization on the prepared guanine peptide nucleic acid self-assembled nano sphere-based photonic crystal.
The transmission spectrum of the visible light region under different incident angles is shown in fig. 11, and the curve of the relationship between the transmission peak and the variation of the bending angle of the guanine peptide nucleic acid self-assembled nano sphere-based photonic crystal and the theoretical value is shown in fig. 12.
According to the embodiment, the acoustic field-assisted preparation of the guanine peptide nucleic acid self-assembled nano sphere-based photonic crystal is realized, the operation is simple and convenient, the process conditions are mild, the process device is simple, and the prepared guanine peptide nucleic acid self-assembled nano sphere-based photonic crystal has excellent biocompatibility and optical characteristics.
Claims (9)
1. A sound field auxiliary preparation method of guanine peptide nucleic acid self-assembly nanosphere-based photonic crystals is characterized by comprising the following steps: the method is characterized in that the guanine peptide nucleic acid self-assembly nanospheres are prepared by adopting a bulk acoustic wave sound field auxiliary mode, and the guanine peptide nucleic acid self-assembly nanospheres are formed by self-assembling guanine peptide nucleic acid.
2. The sound field-assisted preparation method of the guanine peptide nucleic acid self-assembled nanosphere-based photonic crystal according to claim 1, wherein the sound field-assisted preparation method comprises the following steps: the method adopts a bulk acoustic wave transducer to form a bulk acoustic wave sound field auxiliary mode.
3. The sound field-assisted preparation method of the guanine peptide nucleic acid self-assembled nanosphere-based photonic crystal according to claim 1, wherein the sound field-assisted preparation method comprises the following steps: the guanine peptide nucleic acid self-assembly nanosphere-based photonic crystal is packaged by polyethylene glycol diacrylate with the average molecular weight of 200.
4. The sound field-assisted preparation method of the guanine peptide nucleic acid self-assembled nanosphere-based photonic crystal according to claim 1, wherein the sound field-assisted preparation method comprises the following steps: the preparation method specifically comprises the following steps:
1) dissolving guanine peptide nucleic acid powder in ultrapure water, standing at a constant temperature of 25 ℃ and a constant humidity of 80% for 36 hours to prepare guanine peptide nucleic acid self-assembly nanosphere suspension;
2) the substrate is arranged at the bottom in the bulk acoustic wave transducer in advance, turbid liquid is pumped into a cavity of the bulk acoustic wave transducer, and a signal generator of the bulk acoustic wave transducer is turned on to adjust excitation frequency to form a standing wave field in the cavity;
3) standing until the guanine peptide nucleic acid self-assembly nanospheres form stable arrangement in a bulk acoustic wave standing field, and then pumping out redundant turbid liquid in the chamber to enable the guanine peptide nucleic acid self-assembly nanospheres to deposit on the surface of the substrate, thereby obtaining the guanine peptide nucleic acid self-assembly nanosphere-based photonic crystal.
5. The sound field-assisted preparation method of the guanine peptide nucleic acid self-assembled nanosphere-based photonic crystal according to claim 1 or 4, wherein the sound field-assisted preparation method comprises the following steps: the guanine peptide nucleic acid self-assembly nano sphere-based photonic crystal adopts a glass sheet, a quartz sheet, a silicon wafer or a flexible substrate as a substrate.
6. The sound field-assisted preparation method of the guanine peptide nucleic acid self-assembled nanosphere-based photonic crystal according to claim 4, wherein the sound field-assisted preparation method comprises the following steps: the morphology regulation of the guanine peptide nucleic acid self-assembly nanosphere is realized by regulating the molecular structure of the guanine peptide nucleic acid.
7. The sound field-assisted preparation method of the guanine peptide nucleic acid self-assembled nanosphere-based photonic crystal according to claim 1, wherein the sound field-assisted preparation method comprises the following steps: the guanine peptide nucleic acid is an amino acid-like structure with guanine as a side chain group and an amido bond as a molecular skeleton, and the groups for artificially synthesizing the guanine peptide nucleic acid are diphenyl methoxycarbonyl (Bhoc), 9-fluorenylmethoxycarbonyl (Fmoc), glycine, aminoethyl, acetyl and guanine groups.
8. The sound field-assisted preparation method of the guanine peptide nucleic acid self-assembled nanosphere-based photonic crystal according to claim 1 or 2, wherein the sound field-assisted preparation method comprises the following steps: the guanine peptide nucleic acid self-assembly nanospheres are arranged in the bulk acoustic wave transducer in an auxiliary mode through a bulk acoustic wave standing wave field, and the shape formed by arrangement is a one-dimensional stripe shape, a two-dimensional lattice shape or a two-dimensional curve shape.
9. The sound field-assisted preparation method of the guanine peptide nucleic acid self-assembled nanosphere-based photonic crystal according to claim 1 or 3, wherein the sound field-assisted preparation method comprises the following steps: the guanine peptide nucleic acid self-assembly nanosphere-based photonic crystal is further processed by adopting the following processes:
1) weighing polyethylene glycol diacrylate with the average molecular weight of 200, and adding a mixed photoinitiator TPO-L accounting for 0.8% of the mass fraction of the polyethylene glycol diacrylate to form a mixture;
2) fully mixing and defoaming the mixture by using a planetary stirrer to prepare and form a photosensitive prepolymer;
3) placing the substrate attached with the guanine peptide nucleic acid self-assembly nano sphere-based photonic crystal on a spin coating instrument, uniformly dripping a photosensitive prepolymer on the surface of the guanine peptide nucleic acid self-assembly nano sphere-based photonic crystal, forming a uniform film on the surface of the guanine peptide nucleic acid self-assembly nano sphere-based photonic crystal by using a spin coating method, and curing by using ultraviolet light to finish the packaging of the guanine peptide nucleic acid self-assembly nano sphere-based photonic crystal.
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