CN114324520B - Preparation method of photoelectrochemical sensor based on silver sulfide enhanced PTCA glutathione detection - Google Patents
Preparation method of photoelectrochemical sensor based on silver sulfide enhanced PTCA glutathione detection Download PDFInfo
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Abstract
The invention relates to a preparation method of a photoelectrochemical sensor based on silver sulfide enhanced PTCA glutathione detection. According to the invention, PTCA sensitized by silver sulfide quantum dots is used as a substrate material to obtain cathode photocurrent, and PTCA is an organic nanomaterial with excellent photoelectric activity, and after the silver sulfide quantum dots are sensitized, photocurrent response is greatly increased. The glutathione to be detected is directly dissolved in the test electrolyte solution, so that the detection sensitivity is effectively improved, and the sensitive detection of the glutathione is realized. The detection limit is 0.05 nmol/L.
Description
Technical Field
The invention relates to a preparation method of a photoelectrochemical sensor based on silver sulfide enhanced PTCA glutathione detection. Specifically, a silver sulfide quantum dot sensitized PTCA nano sheet is used as a substrate photosensitive material to obtain cathode photocurrent, and glutathione to be detected is directly dissolved in a test electrolyte solution to prepare a photoelectrochemical sensor for sensitively detecting glutathione, and the photoelectrochemical sensor belongs to the technical field of novel functional materials and sensing detection.
Background
Glutathione (GSH) is a tripeptide that naturally occurs in all areas of life. Glutathione helps to maintain normal immune system function, and has antioxidant and integral detoxification effects. The sulfhydryl group on the cysteine is an active group of the cysteine, and is easy to combine with certain medicines, toxins and the like, so that the cysteine has the functions of integration and detoxification. The glutathione can be used for medicines and can be used as a base material of functional foods, and can be widely applied to the functional foods for delaying aging, enhancing immunity, resisting tumors and the like. Glutathione is a common biomarker and can be used for detection and diagnosis of a plurality of diseases. Although photoby glutathione concentration is not diagnostic for disease, it is still an indicator of possible health problems. When glutathione levels are significantly elevated, the patient's chances of being diagnosed with a neurodegenerative disease increases. It would be beneficial to be able to accurately detect and quantify glutathione in biological samples, as the number of cases of these diseases increases. Over time, it is also possible to determine the progression of diseases such as parkinson's disease or alzheimer's disease by diagnostic means. Therefore, it is necessary to establish an analytical method for detecting glutathione sensitively and accurately. Common methods for detecting glutathione include fluorescence analysis, colorimetric analysis, electrochemical analysis, and the like. However, the fluorescence analysis method is often complicated to operate and has a narrow detection linear range; the colorimetric analysis detection error is large; the electrochemical analysis time is long. The invention designs a novel photoelectrochemical sensor to realize sensitive detection of glutathione, and has the advantages of high analysis speed, simple operation and good stability, and the detection limit of the photoelectrochemical sensor designed by the invention on the glutathione reaches 0.05 nmol/L.
TPCA (3, 4,9, 10-perylene tetracarboxylic dianhydride), an excellent organic semiconductor nanomaterial, has good photocatalytic performance and photoelectric performance, is simple in preparation method, nontoxic, has certain photostability and thermal stability, can generate photo-generated charges under visible light irradiation, and further forms photocurrent, but the photoelectric conversion efficiency of the pure TPCA nanomaterial is not high. Silver sulfide is used as an excellent sensitization material, and has the advantages of simple preparation, high yield and good biocompatibility. The flaky PTCA has large specific surface area, and can carry a large amount of silver sulfide quantum dots by sensitization of the silver sulfide quantum dots, so that excellent photoelectric performance is obtained. The substrate photosensitive material generates a cathode photoelectric signal, and the cathode photoelectric signal can effectively avoid the interference of certain reducing substances, so that the detection is more accurate. The glutathione to be detected is directly dissolved in the photoelectrochemical test electrolyte solution, and is used as an electron donor in the test process, and the photocurrent signal obtained by the test is gradually reduced along with the increase of the concentration of the glutathione, so that the detection sensitivity of the sensor is improved, and the detection sensitivity of the sensor is greatly improved.
Photoelectrochemical sensors are a type of detection device that determines the concentration of an analyte based on the photoelectric conversion characteristics of the substance. The photoelectrochemistry detection method has the characteristics of simple equipment, high sensitivity and easy miniaturization, has been developed into an analysis method with great application potential, and has wide application prospect in the fields of food, environment, medicine and the like. The application of the PTCA nanomaterial sensitized by silver sulfide quantum dots in the photoelectrochemical sensor is not reported. The invention successfully constructs the photoelectrochemical sensor for detecting the glutathione under the visible light based on the PTCA nano material sensitized by the silver sulfide quantum dots. According to the sensor, silver sulfide quantum dot sensitized sheet PTCA is used as a substrate photosensitive material, and glutathione to be detected is directly dissolved in a test electrolyte solution, so that sensitive detection of the glutathione is realized. The photoelectrochemical sensor prepared by the invention has the advantages of low cost, high sensitivity, good specificity, rapid detection, easy preparation and the like, realizes the rapid and high-sensitivity detection of glutathione in a visible light region, and effectively overcomes the defects of the current glutathione detection method.
Disclosure of Invention
One of the purposes of the invention is to utilize PTCA nano-material sensitized by silver sulfide quantum dots as photosensitive material. The photosensitive material has excellent photoelectric performance and extremely high photoelectric conversion efficiency under visible light.
The second purpose of the invention is to directly dissolve the glutathione of the object to be tested in the test base solution, so that the sensitivity of the detection is greatly improved.
The third purpose of the invention is to prepare the photoelectrochemical sensor with high sensitivity, good stability and high detection speed by taking the silver sulfide-PTCA nano material as a substrate, thereby realizing the purpose of sensitively detecting the glutathione under the condition of visible light.
The technical scheme of the invention is as follows:
1. the preparation method of the photoelectrochemical sensor based on the detection of the silver sulfide enhanced PTCA glutathione is characterized by comprising the following steps of:
(1) Preparation of PTCA nanomaterials
Dissolving 5-20 mg perylene tetracarboxylic dianhydride in 10 mL concentration 1-3 mol/L sodium hydroxide aqueous solution, heating until the solid is completely dissolved to obtain a yellowish green solution, adding 1-5 mol/L hydrochloric acid aqueous solution into the solution until the color of the solution changes to brick red, continuously stirring the obtained brick red solution at room temperature for 10-30 min, washing a brick red product with absolute ethyl alcohol and ultrapure water, and vacuum drying at 10-40 ℃ to obtain the PTCA nanomaterial;
(2) Preparation of silver sulfide quantum dot solution
Dissolving 0.3-0.7 g silver nitrate in a glycol solution of 15-55 mL, stirring uniformly at room temperature, adding a 1-5 mL thioglycollic acid solution into the solution, heating the mixed solution to 100-160 ℃, continuously stirring for 30-60 min, changing the color of the solution from yellow to black, cooling to room temperature, washing the obtained product with absolute ethyl alcohol, dispersing in water, and storing in a refrigerator at 4 ℃ for standby to obtain a silver sulfide quantum dot solution;
(3) Preparation of photoelectrochemical sensor
1) Ultrasonically cleaning conductive glass sequentially by using a surfactant, acetone, ethanol and ultrapure water, introducing nitrogen gas into a 70 ℃ oven for drying;
2) Dripping 25 mu L of PTCA aqueous solution with the concentration of 1-5 mg/mL on the conductive surface of the ITO conductive glass, and baking under an infrared lamp;
3) Continuously dripping 5-10 mu L of silver sulfide quantum dot solution on the surface of the modified electrode, and naturally airing at room temperature in a dark place; a photosensitive electrode for detecting glutathione is prepared.
2. The method for detecting a photoelectrode produced by the production method according to claim 1, comprising the steps of:
(1) Using an electrochemical workstation to test by using a three-electrode system, wherein a saturated calomel electrode is used as a reference electrode, a platinum electrode is used as an auxiliary electrode, and the prepared ITO modified photosensitive electrode is used as a working electrode, and is tested in a PBS buffer solution which contains 0.1-500 nmol/L glutathione and has a pH value of 5.0-8.0;
(2) Detecting glutathione by a time-current method, setting the voltage to be-0.1V, the running time to be 120 s, and the wavelength of a light source to be 400-450 nm;
(3) After the electrodes are placed, the lamp is turned on every 10 s to continuously irradiate 10 s, record photocurrent, and draw a working curve;
(4) And (3) detecting the glutathione sample solution to be detected instead of the glutathione standard solution.
The linear range of the sensor for detecting the glutathione is 0.1-500 nmol/L, and the detection limit is 0.05 nmol/L.
The chemicals required for the synthesis of the materials were purchased from local reagent shops and were not reprocessed.
Advantageous results of the invention
(1) The invention successfully synthesizes the flaky PTCA nano material with certain photoelectric property, and the material has low preparation cost, no toxicity and large specific surface area; the PTCA is sensitized by utilizing the good sensitization of the silver sulfide, so that the excellent photoelectric performance is obtained, and the problem of low photoelectric conversion efficiency of the pure PTCA and the pure silver sulfide is solved.
(2) According to the invention, the to-be-detected object is directly dissolved in the photoelectrochemical test electrolyte solution, and the photoelectric signal is gradually reduced along with the increase of the concentration of the to-be-detected object, so that the sensitive detection of the glutathione is realized.
(4) The photoelectrochemical sensor prepared by the invention is used for detecting glutathione, has short response time, wide linear range, low detection limit, good stability and reproducibility, and can realize simple, rapid, high-sensitivity and specific detection. The linear range of the detection of the glutathione is 0.1-500 nmol/L, and the detection limit is 0.05 nmol/L.
Detailed description of the preferred embodiments
Example 1 preparation of photoelectrochemical sensor
(1) Preparation of PTCA nanomaterials
Dissolving 5 mg perylene tetracarboxylic dianhydride in 10 mL concentration 1 mol/L sodium hydroxide aqueous solution, heating until the solid is completely dissolved to obtain a yellowish green solution, gradually adding 1 mol/L hydrochloric acid aqueous solution into the solution until the color of the solution becomes brick red, continuously stirring the obtained brick red solution at room temperature for 10 min, washing a brick red product with absolute ethyl alcohol and ultrapure water, and vacuum drying at 10 ℃ to obtain the PTCA nanomaterial;
(2) Preparation of silver sulfide quantum dot solution
Dissolving 0.3 g silver nitrate in 15 mL glycol solution, stirring uniformly at room temperature, adding 1 mL thioglycollic acid solution into the solution, heating the mixed solution to 100 ℃, continuously stirring for 30 min, changing the color of the solution from yellow to black, cooling to room temperature, washing the obtained product with absolute ethyl alcohol, dispersing in water, and carrying out shading preservation in a refrigerator at 4 ℃ for later use to obtain silver sulfide quantum dot solution;
(3) Preparation of photoelectrochemical sensor
1) Ultrasonically cleaning conductive glass sequentially by using a surfactant, acetone, ethanol and ultrapure water, introducing nitrogen gas into a 70 ℃ oven for drying;
2) Dripping 25 mu L of PTCA aqueous solution with the concentration of 1 mg/mL on the conductive surface of the ITO conductive glass, and baking under an infrared lamp;
3) Continuously dripping 5 mu L of silver sulfide quantum dot solution on the modified electrode surface, and naturally airing at room temperature in a dark place; a photosensitive electrode for detecting glutathione is prepared.
Example 2 preparation of photoelectrochemical sensor
(1) Preparation of PTCA nanomaterials
Dissolving 10 mg perylene tetracarboxylic dianhydride in a 2 mol/L sodium hydroxide aqueous solution with the concentration of 10 mL, heating until the solid is completely dissolved to obtain a yellowish green solution, gradually adding a 4 mol/L hydrochloric acid aqueous solution into the solution until the color of the solution becomes brick red, continuously stirring the obtained brick red solution at room temperature for 20 min, washing a brick red product with absolute ethyl alcohol and ultrapure water, and vacuum drying at 30 ℃ to obtain the PTCA nanomaterial;
(2) Preparation of silver sulfide quantum dot solution
Dissolving 0.5 g silver nitrate in a 35 mL ethylene glycol solution, stirring uniformly at room temperature, adding a 4 mL thioglycollic acid solution into the solution, heating the mixed solution to 120 ℃, continuously stirring for 40 min, changing the color of the solution from yellow to black, cooling to room temperature, washing the obtained product with absolute ethyl alcohol, dispersing in water, and carrying out shading preservation in a refrigerator at 4 ℃ for later use to obtain a silver sulfide quantum dot solution;
(3) Preparation of photoelectrochemical sensor
1) Ultrasonically cleaning conductive glass sequentially by using a surfactant, acetone, ethanol and ultrapure water, introducing nitrogen gas into a 70 ℃ oven for drying;
2) Dripping 25 mu L of PTCA aqueous solution with the concentration of 3 mg/mL on the conductive surface of the ITO conductive glass, and baking under an infrared lamp;
3) Continuously dripping 6 mu L of silver sulfide quantum dot solution on the modified electrode surface, and naturally airing at room temperature in a dark place; a photosensitive electrode for detecting glutathione is prepared.
Example 3 preparation of photoelectrochemical sensor
(1) Preparation of PTCA nanomaterials
Dissolving 20 mg perylene tetracarboxylic dianhydride in 10 mL sodium hydroxide aqueous solution with the concentration of 3 mol/L, heating until the solid is completely dissolved to obtain a yellowish green solution, gradually adding 5 mol/L hydrochloric acid aqueous solution into the solution until the color of the solution becomes brick red, continuously stirring the obtained brick red solution at room temperature for 30 min, washing a brick red product with absolute ethyl alcohol and ultrapure water, and vacuum drying at 40 ℃ to obtain the PTCA nanomaterial;
(2) Preparation of silver sulfide quantum dot solution
Dissolving 0.7 g silver nitrate in 55 mL glycol solution, stirring uniformly at room temperature, adding 5 mL thioglycollic acid solution into the solution, heating the mixed solution to 160 ℃, continuously stirring for 60 min, changing the color of the solution from yellow to black, cooling to room temperature, washing the obtained product with absolute ethyl alcohol, dispersing in water, and shading and preserving in a refrigerator at 4 ℃ for later use to obtain silver sulfide quantum dot solution;
(3) Preparation of photoelectrochemical sensor
1) Ultrasonically cleaning conductive glass sequentially by using a surfactant, acetone, ethanol and ultrapure water, introducing nitrogen gas into a 70 ℃ oven for drying;
2) Dripping 25 mu L of PTCA aqueous solution with the concentration of 5 mg/mL on the conductive surface of the ITO conductive glass, and baking under an infrared lamp;
3) Continuously dripping 10 mu L of silver sulfide quantum dot solution on the modified electrode surface, and naturally airing at room temperature in a dark place; a photosensitive electrode for detecting glutathione is prepared.
Example 4 detection of glutathione
(1) Using an electrochemical workstation to test by using a three-electrode system, wherein a saturated calomel electrode is used as a reference electrode, a platinum electrode is used as an auxiliary electrode, and the prepared ITO modified photosensitive electrode is used as a working electrode, and is tested in a PBS buffer solution with pH of 5.0 and containing 0.1-500 nmol/L glutathione;
(2) Detecting glutathione by a time-current method, setting the voltage to be-0.1 and V, and the running time to be 120 s, wherein the light source wavelength is 400 nm;
(3) After the electrodes are placed, the lamp is turned on every 10 s to continuously irradiate 10 s, record photocurrent, and draw a working curve;
(4) And (3) detecting the glutathione sample solution to be detected instead of the glutathione standard solution.
Example 5 detection of glutathione
(1) Using an electrochemical workstation to test by using a three-electrode system, wherein a saturated calomel electrode is used as a reference electrode, a platinum electrode is used as an auxiliary electrode, and the prepared ITO modified photosensitive electrode is used as a working electrode, and is tested in a PBS buffer solution with pH of 7.0 and containing 0.1-500 nmol/L glutathione;
(2) Detecting glutathione by a time-current method, setting the voltage to be 0V, the running time to be 120 s and the light source wavelength to be 420 nm;
(3) After the electrodes are placed, the lamp is turned on every 10 s to continuously irradiate 10 s, record photocurrent, and draw a working curve;
(4) And (3) detecting the glutathione sample solution to be detected instead of the glutathione standard solution.
Example 6 detection of glutathione
(1) Using an electrochemical workstation to test by using a three-electrode system, wherein a saturated calomel electrode is used as a reference electrode, a platinum electrode is used as an auxiliary electrode, and the prepared ITO modified photosensitive electrode is used as a working electrode, and is tested in a PBS buffer solution with pH of 7.5 and containing 0.1-500 nmol/L glutathione;
(2) Detecting glutathione by a time-current method, setting the voltage to be-0.1V, and the running time to be 120 s, wherein the light source wavelength is 430 nm;
(3) After the electrodes are placed, the lamp is turned on every 10 s to continuously irradiate 10 s, record photocurrent, and draw a working curve;
(4) And (3) detecting the glutathione sample solution to be detected instead of the glutathione standard solution.
Claims (2)
1. The preparation method of the photoelectrochemical sensor based on the detection of the silver sulfide enhanced PTCA glutathione is characterized by comprising the following steps of:
(1) Preparation of PTCA nanomaterials
Dissolving 5-20 mg perylene tetracarboxylic dianhydride in 10 mL concentration 1-3 mol/L sodium hydroxide aqueous solution, heating until the solid is completely dissolved to obtain a yellowish green solution, adding 1-5 mol/L hydrochloric acid aqueous solution into the solution until the color of the solution changes to brick red, continuously stirring the obtained brick red solution at room temperature for 10-30 min, washing a brick red product with absolute ethyl alcohol and ultrapure water, and vacuum drying at 10-40 ℃ to obtain the PTCA nanomaterial;
(2) Preparation of silver sulfide quantum dot solution
Dissolving 0.3-0.7 g silver nitrate in a glycol solution of 15-55 mL, stirring uniformly at room temperature, adding a 1-5 mL thioglycollic acid solution into the solution, heating the mixed solution to 100-160 ℃, continuously stirring for 30-60 min, changing the color of the solution from yellow to black, cooling to room temperature, washing the obtained product with absolute ethyl alcohol, dispersing in water, and storing in a refrigerator at 4 ℃ for standby to obtain a silver sulfide quantum dot solution;
(3) Preparation of photoelectrochemical sensor
1) Ultrasonically cleaning conductive glass sequentially by using a surfactant, acetone, ethanol and ultrapure water, introducing nitrogen gas into a 70 ℃ oven for drying;
2) Dripping 25 mu L of PTCA aqueous solution with the concentration of 1-5 mg/mL on the conductive surface of the ITO conductive glass, and baking under an infrared lamp;
3) Continuously dripping 5-10 mu L of silver sulfide quantum dot solution on the surface of the modified electrode, and naturally airing at room temperature in a dark place; a photosensitive electrode for detecting glutathione is prepared.
2. The method for detecting a photoelectrode produced by the production method according to claim 1, comprising the steps of:
(1) Using an electrochemical workstation to test by using a three-electrode system, wherein a saturated calomel electrode is used as a reference electrode, a platinum electrode is used as an auxiliary electrode, and the prepared ITO modified photosensitive electrode is used as a working electrode, and is tested in a PBS buffer solution which contains 0.1-500 nmol/L glutathione and has a pH value of 5.0-8.0;
(2) Detecting glutathione by a time-current method, setting the voltage to be-0.1V, the running time to be 120 s, and the wavelength of a light source to be 400-450 nm;
(3) After the electrodes are placed, the lamp is turned on every 10 s to continuously irradiate 10 s, record photocurrent, and draw a working curve;
(4) And (3) detecting the glutathione sample solution to be detected instead of the glutathione standard solution.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| RU2528584C1 (en) * | 2013-03-19 | 2014-09-20 | Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Национальный исследовательский Томский политехнический университет" | Method of determining glutathione in standard test aqueous solutions via cyclic voltammetry on graphite electrode, modified with colloidal gold particles |
| CN109060898A (en) * | 2018-06-28 | 2018-12-21 | 济南大学 | Based on CeO2The preparation method of the brain natriuretic peptide antigen optical electro-chemistry sensor of-CdS attenuated type |
| CN111665288A (en) * | 2020-07-15 | 2020-09-15 | 常州大学 | Preparation method and application method of working electrode for detecting glutathione by electrochemical luminescence method |
| CN113092452A (en) * | 2021-04-23 | 2021-07-09 | 济南大学 | Preparation method and application of biochemical sensor |
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| CN102277157B (en) * | 2011-05-30 | 2014-06-11 | 中国科学院苏州纳米技术与纳米仿生研究所 | Near-infrared silver sulphide quantum dot as well as preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| RU2528584C1 (en) * | 2013-03-19 | 2014-09-20 | Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Национальный исследовательский Томский политехнический университет" | Method of determining glutathione in standard test aqueous solutions via cyclic voltammetry on graphite electrode, modified with colloidal gold particles |
| CN109060898A (en) * | 2018-06-28 | 2018-12-21 | 济南大学 | Based on CeO2The preparation method of the brain natriuretic peptide antigen optical electro-chemistry sensor of-CdS attenuated type |
| CN111665288A (en) * | 2020-07-15 | 2020-09-15 | 常州大学 | Preparation method and application method of working electrode for detecting glutathione by electrochemical luminescence method |
| CN113092452A (en) * | 2021-04-23 | 2021-07-09 | 济南大学 | Preparation method and application of biochemical sensor |
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