CN114324508A - Sweat lactic acid detection device and method - Google Patents
Sweat lactic acid detection device and method Download PDFInfo
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Abstract
The invention discloses a sweat lactic acid detection device and a sweat lactic acid detection method, wherein the device comprises a collection module, a sensing module and a processing module, wherein the collection module is used for collecting sweat; the sensing module is used for collecting the lactic acid content, the pH value and the environmental temperature of sweat and correspondingly generating lactic acid content information, pH value information and environmental temperature information, the lactic acid sensor comprises a working electrode and a reference electrode, and a gold layer is arranged on the surface of the working electrode; the processing module is used for correcting the lactic acid content information according to the pH value information and the environment temperature information to obtain lactic acid content data of sweat; the invention uses sweat to replace blood as diagnostic solution to detect the content of lactic acid in human body, realizes non-invasive detection of lactic acid, is suitable for real-time detection, has high detection accuracy and strong stability, and is convenient to carry and execute.
Description
Technical Field
The invention relates to the technical field of sweat lactic acid detection, in particular to a sweat lactic acid detection device and method.
Background
Sweat is an important secretion of human metabolism, is rich in abundant physiological information, and does not need to cause harm to a human body when being obtained. In addition, sweat contains abundant physiological and metabolic biomarkers, which are indicators of human health and performance, and are closely related to plasma levels, and thus can be used as an ideal substitute diagnostic solution for blood.
Lactic acid is the final product of anaerobic glycolysis, and when oxygen supply is insufficient, lactic acid accumulates, thereby causing lactic acidosis.
However, limited by the prior art, most of the existing lactate detection methods use invasive detection methods, which require the collection of the subject's blood to determine lactate content. The invasive detection method has high equipment cost and long detection time, and in addition, the invasive collection of the blood of a subject is required, so that the pain of the subject is undoubtedly brought. In addition, the invasive detection method cannot meet the requirement of people for detecting the content change of the lactic acid in the body in real time, and greatly limits the development of sweat lactic acid detection technology.
Disclosure of Invention
The invention aims to provide a sweat lactic acid detection device and method, which use sweat to replace blood as diagnostic solution to detect the content of lactic acid in a human body, realize non-invasive detection of lactic acid, are suitable for real-time detection, have high detection accuracy and strong stability, are convenient to carry and execute, and avoid the pain of a person to be detected caused by the invasive method of blood collection.
In order to achieve the above object, the present invention discloses a sweat lactate detection device, which comprises a collection module, a sensing module and a processing module, wherein the collection module is used for collecting sweat; the sensing module comprises a lactic acid sensor, a pH value (PH value) sensor and a temperature sensor, the lactic acid sensor, the pH value sensor and the temperature sensor are respectively used for collecting the lactic acid content, the pH value and the environmental temperature of the sweat and correspondingly generating lactic acid content information, pH value information and environmental temperature information, the lactic acid sensor comprises a working electrode and a reference electrode, a gold layer is arranged on the surface of the working electrode, and a silver layer is arranged on the surface of the reference electrode; the processing module is used for correcting the lactic acid content information according to the pH value information and the environmental temperature information so as to obtain lactic acid content data of the sweat.
Compared with the prior art, on one hand, the method uses sweat to replace blood as diagnostic solution to detect the content of the lactic acid in the human body, realizes the non-invasive detection of the lactic acid, can obtain the sweat in real time through non-invasive, is suitable for real-time detection, has high detection accuracy and strong stability, and is convenient to carry and execute; on the other hand, the sweat is collected in a non-invasive manner, so that the pain of the examinee caused by the fact that the diagnosis liquid is obtained through an invasive method of blood collection in the prior art is avoided, and the detection experience is effectively improved; on the other hand, the processing module corrects the lactic acid content information according to the pH value information and the environmental temperature information to obtain the lactic acid content data of the sweat, and detection abnormity caused by pH value and/or environmental temperature abnormity is avoided in a combined correction mode.
Preferably, gold nano pine needles are deposited on the surface of the working electrode.
Preferably, the lactate sensor further comprises lactate oxidase and a cross-linking agent, wherein the lactate oxidase is covalently cross-linked with the working electrode and the reference electrode through the cross-linking agent.
Specifically, the crosslinking agent is polyethylene glycol diglycidyl ether.
Preferably, the collection module comprises a hydrophobic membrane and a plurality of absorption tubes, the hydrophobic membrane is of a hollow structure, the hollow structure forms a detection cavity, the absorption tubes are distributed at intervals on the periphery of the detection cavity and are respectively communicated with the detection cavity, and the absorption tubes are used for draining the sweat to the detection cavity.
Preferably, the processing module is configured to perform filtering and amplification processing on the lactic acid content information, the ph information, and the environmental temperature information.
Correspondingly, the invention also discloses a sweat lactic acid detection method which is applied to the sweat lactic acid detection device and comprises the following steps:
s1, collecting sweat;
s2, collecting the lactic acid content, the pH value and the environmental temperature of the sweat and correspondingly generating lactic acid content information, pH value information and environmental temperature information, wherein the lactic acid content of the sweat is collected through a lactic acid sensor, the lactic acid sensor comprises a working electrode and a reference electrode, a gold layer is arranged on the surface of the working electrode, and a silver layer is arranged on the surface of the reference electrode;
s3, filtering and amplifying the lactic acid content information, the pH value information and the environment temperature information;
and S4, correcting the lactic acid content information according to the pH value information and the environmental temperature information to obtain lactic acid content data of the sweat.
Preferably, the working electrode and the reference electrode are both prepared by adopting an electron beam evaporation coating process.
Preferably, the working electrode is prepared by adopting an electron beam evaporation coating process, and the method comprises the following steps:
providing a first flexible electrode;
electroplating a titanium layer with the thickness of 4nm to 6nm on the surface of the first flexible electrode;
and electroplating a gold layer with the thickness of 55nm to 65nm on the surface of the titanium layer to obtain the working electrode.
Preferably, the reference electrode is prepared by an electron beam evaporation coating process, which comprises the following steps:
providing a second flexible electrode;
electroplating a titanium layer with the thickness of 4nm to 6nm on the surface of the second flexible electrode;
electroplating a silver layer with the thickness of 190nm to 210nm on the surface of the titanium layer;
soaking the second flexible electrode in a 3M potassium chloride solution;
and carrying out chlorination treatment on the second flexible electrode by a chronopotentiometric process to prepare the reference electrode.
Preferably, the step of correcting the lactic acid content information according to the ph information and the environmental temperature information to obtain the lactic acid content data of the sweat includes:
and calculating to obtain a corrected real lactic acid content value according to a formula L (C-Kt T + C0)/(ph Kp + K0), wherein L is the corrected real lactic acid content value, C is a current measured value of the lactic acid sensor, Kt is the sensitivity (slope) of the current measured value of the lactic acid sensor along with the change of temperature, T is the body temperature, C0 is the current measured initial value of the lactic acid sensor, ph is the current measured value of the pH value sensor, Kp is the slope of the sensitivity of the current measured value of the lactic acid sensor along with the change of the real lactic acid value along with the change of the ph value, and K0 is the current change sensitivity initial value of the lactic acid sensor.
Preferably, the electroplating a gold layer with the thickness of 55nm to 65nm on the surface of the titanium layer to prepare the working electrode, and then further comprises:
soaking the working electrode in a sodium chloride solution with the concentration of the tetrachloroauric acid between 2.5mg/mL and 3.5 mg/mL;
and carrying out deposition treatment on the working electrode by an electrochemical deposition process so as to deposit and form the gold nano pine needles on the surface of the working electrode.
Drawings
FIG. 1 is a schematic diagram of the configuration of a sweat lactate detection device of the present invention;
FIG. 2 is a block flow diagram of the sweat lactate detection method of the present invention;
FIG. 3 is a graphical representation of lactate content of sweat of the present invention as a function of current measurements from a lactate sensor;
FIG. 4 is a graphical representation of pH of sweat of the present invention as a function of current measurements from a lactate sensor;
fig. 5 is a graphical representation of the ambient temperature of sweat of the present invention as a function of current measurements from a lactate sensor.
Detailed Description
In order to explain technical contents, structural features, and objects and effects of the present invention in detail, the following detailed description is given with reference to the accompanying drawings in conjunction with the embodiments.
Referring to fig. 1, the sweat lactate detection device of the present embodiment can be applied to detect lactate content data of a human body in real time, so as to reflect the exercise amount and metabolic capability of a subject in real time through the lactate content data. This sweat lactic acid detection device is including collecting module 10, sensing module and processing module 30, and wherein, collecting module 10 is used for collecting the sweat, and sensing module connects collecting module 10 to carry out data acquisition to the sweat that obtains collecting, processing module 30 is connected in the sensing module communication, with the information transmission who gathers to processing module 30, for carrying out data processing.
Preferably, this collection module 10 includes hydrophobic membrane and four absorption tubes, and hydrophobic membrane is hollow structure, and hollow structure forms and detects the chamber, and four absorption tubes are the week side that distributes at the detection chamber at interval to communicate respectively and detect the chamber, the absorption tube is used for draining the sweat to detecting the chamber. Of course, the number of the adsorption tubes is flexibly selected depending on the size of the hydrophobic membrane, and thus the number of the adsorption tubes is not particularly limited herein.
Preferably, the hydrophobic membrane and the absorption tube are made of hydrophobic materials. The hydrophobic material is a low surface energy coating with a static water contact angle theta of more than 90 degrees on a smooth surface of a coating film, has important characteristics of hydrophobicity, water resistance, fog resistance, snow resistance, pollution prevention, adhesion resistance, oxidation resistance, corrosion resistance, self-cleaning, current conduction prevention and the like, and is suitable for being used as the hydrophobic material, such as Polytetrafluoroethylene (PTFE), Polydimethylsiloxane (PDMS), polyvinylidene fluoride (PVDF), polypropylene (PP), Polyethylene (PE), polymethyl methacrylate (PMMA), Polyolefin (PO), Polyamide (PA), Polyacrylonitrile (PAN), Polyester (PET), Polycarbonate (PC), fluorosilicone resin, molten paraffin, organic silicon wax emulsion and the like.
The sensing module is a multi-channel sensor assembly, and comprises a lactic acid sensor 21, an acid-base number sensor 22 and a temperature sensor 23. The lactic acid sensor 21 is used for collecting the lactic acid content of the sweat and generating lactic acid content information, the ph sensor 22 is used for collecting the ph of the sweat and generating ph information, and the temperature sensor 23 is used for collecting the environmental temperature of the sweat and generating environmental temperature information, where the environmental temperature may also be a testing environmental temperature. Preferably, the metal probe of the temperature sensor 23 is made of metal chromium or gold to improve the temperature detection accuracy
The lactate sensor 21 includes a working electrode and a reference electrode, a gold layer is disposed on the surface of the working electrode, and a silver layer is disposed on the surface of the reference electrode.
The processing module 30 is configured to modify the lactic acid content information according to the ph information and the environmental temperature information to obtain lactic acid content data of sweat. Preferably, the processing module 30 is further configured to perform filtering and amplifying processing on the lactic acid content information, the ph information and the environmental temperature information to remove interference signals and convert the information into current signals suitable for processing. It will be appreciated that the filter amplification process step precedes the modification process so that the filtered amplified current signal can be better used in the modification process.
Further, the processing module 30 may be externally connected to a device terminal, and the processing module 30 sends the lactic acid content data of the sweat to the device terminal for performing the subsequent steps, where the device terminal is a local terminal or a remote terminal. Of course, in other preferred modes, the processing module 30 and the device terminal may be the same processing device, so as to meet the design requirements of more device schemes.
Preferably, gold nano pine needles (Au NNs) are deposited on the surface of the working electrode to amplify the lactic acid content signal contained in the collected sweat, thereby improving the collection sensitivity of the working electrode.
Preferably, the lactate sensor 21 further comprises lactate oxidase and a cross-linking agent, and the lactate oxidase is covalently cross-linked with the working electrode and the reference electrode by the cross-linking agent. Specifically, the crosslinking agent is polyethylene glycol diglycidyl ether.
It is understood that sweat passing through the lactate sensor 21 is oxidized by lactate oxidase to undergo redox reaction to generate a potential change, and the potential change is detected by the conductive elements of the working electrode and the reference electrode, thereby calculating the corresponding lactate content information. The pole cores of the working electrode and the reference electrode are both flexible electrodes, and in order to enable lactate oxidase to be better fixed on the surface of the flexible electrode and maintain higher enzyme activity, polyethylene glycol diglycidyl ether (PEGDE) is adopted as a cross-linking agent to covalently cross-link the oxidase and the electrodes, so that sweat can be ensured to fully react with the lactate oxidase fixed on the working electrode and the reference electrode, and the accuracy of lactate content detection is ensured.
Referring to fig. 1-5, the present invention also discloses a sweat lactic acid detection method applied to the above sweat lactic acid detection device, wherein the sweat lactic acid detection method includes the following steps:
s1, collecting sweat;
s2, collecting the lactic acid content, the pH value and the environment temperature of sweat and correspondingly generating lactic acid content information, pH value information and environment temperature information, wherein the lactic acid content of the sweat is collected through the lactic acid sensor 21, the lactic acid sensor 21 comprises a working electrode and a reference electrode, a gold layer is arranged on the surface of the working electrode, and a silver layer is arranged on the surface of the reference electrode.
And S3, filtering and amplifying the lactic acid content information, the pH value information and the environment temperature information.
And S4, correcting the lactic acid content information according to the pH value information and the environmental temperature information to obtain the lactic acid content data of the sweat.
Preferably, the working electrode and the reference electrode are both prepared by adopting an electron beam evaporation coating process.
Preferably, the working electrode is prepared by adopting an electron beam evaporation coating process, and the method comprises the following steps:
providing a first flexible electrode;
the titanium layer with the thickness of 4nm to 6nm is electroplated on the surface of the first flexible electrode, and the detection precision can be guaranteed by preferably selecting the thickness of the titanium layer to be 5nm, and the production cost is effectively saved.
And electroplating a gold layer with the thickness of 55nm to 65nm on the surface of the titanium layer to obtain the working electrode. The thickness of the gold layer is preferably 60nm, so that the detection precision can be ensured, and the production cost can be effectively saved.
Preferably, the reference electrode is prepared by adopting an electron beam evaporation coating process, and the method comprises the following steps:
providing a second flexible electrode;
and a titanium layer with the thickness of 4nm to 6nm is electroplated on the surface of the second flexible electrode, and the detection precision can be ensured by preferably selecting the thickness of the titanium layer to be 5nm, and the production cost is effectively saved.
And a silver layer with the thickness of 190nm to 210nm is electroplated on the surface of the titanium layer, and the detection precision can be ensured and the production cost can be effectively saved by preferably selecting the thickness of the titanium layer as 200 nm.
Soaking the second flexible electrode in a 3M potassium chloride solution;
and (3) carrying out chlorination treatment on the second flexible electrode by a chronopotentiometry process to obtain the reference electrode.
Preferably, the step of correcting the lactic acid content information according to the ph information and the environmental temperature information to obtain the lactic acid content data of the sweat includes:
the corrected real lactic acid content value is obtained by calculation according to the formula L ═ C-Kt + C0)/(ph-Kp + K0, where L is the corrected real lactic acid content value, C is the current measurement value of the lactic acid sensor 21, Kt is the sensitivity (slope) of the current measurement value of the lactic acid sensor 21 with respect to temperature change, T is the body temperature, C0 is the current measurement initial value of the lactic acid sensor 21, ph is the ph value of sweat, Kp is the slope of the sensitivity of the current measurement value of the lactic acid sensor 21 with respect to the change of the real lactic acid value with respect to ph value, and K0 is the current change sensitivity initial value of the lactic acid sensor 21.
The above steps are explained below:
fig. 3 shows a schematic diagram of the relationship between the lactate content of sweat and the current measurement of lactate sensor 21, and it can be seen from fig. 3 that the lactate content of sweat and the current measurement of lactate sensor 21 are in a linear relationship and can be expressed by the formula C ═ K × L + C0.
Fig. 4 shows a relationship between the ph of sweat and the current measured by the lactate sensor 21, and as can be seen from fig. 4, the ph of sweat has an effect on K in the formula C K L + C0, which is close to a linear relationship, and in conjunction with fig. 3 and 4, the formula K ph Kp + K0 can be found.
Fig. 5 shows a relationship between the ambient temperature of sweat and the current measurement of the lactate sensor 21, and as can be seen from fig. 5, the ambient temperature of sweat has an effect on K of the formula C K L + C0, which is close to a linear relationship, and in conjunction with fig. 3 and 5, the formula C Kt + Kt0 can be found.
Therefore, the relationship between the current signal generated by the lactate sensor 21 and the ph and temperature of sweat is: c ═ Kl × L + Kt × T + C0, and Kl ═ ph Kp + K0, giving the formula C ═ ph Kp + K0) × L + Kt × T + C0.
Preferably, a gold layer with the thickness of 55nm to 65nm is electroplated on the surface of the titanium layer to prepare a working electrode, and then the method further comprises the following steps:
soaking the working electrode in tetrachloroauric acid (HAuC l)4) Sodium chloride solution with concentration of 2.5mg/mL to 3.5mg/mL, preferably tetrachloroauric acid (HAuCl)4) The subsequent reaction requirement can be met when the concentration is 3.0 mg/mL.
And carrying out deposition treatment on the working electrode by an electrochemical deposition process so as to deposit and form the gold nano pine needles on the surface of the working electrode.
With reference to fig. 1-5, in the aspect of the present invention, sweat is used as a diagnostic solution instead of blood to detect the content of lactic acid in a human body, so as to achieve non-invasive detection of lactic acid, and sweat can be obtained in a non-invasive manner in real time, so that the sweat is suitable for real-time detection, and has high detection accuracy, strong stability, and is convenient to carry and execute; on the other hand, the sweat collection is non-invasive, so that the pain of the examinee caused by the fact that the diagnosis liquid is obtained through an invasive method of blood collection in the prior art is avoided, and the detection experience is effectively improved.
The above disclosure is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the scope of the present invention, therefore, the present invention is not limited by the appended claims.
Claims (10)
1. A sweat lactate detection device, comprising:
the collection module is used for collecting sweat;
the sensing module comprises a lactic acid sensor, an acid-base value sensor and a temperature sensor, wherein the lactic acid sensor, the acid-base value sensor and the temperature sensor are respectively used for collecting the lactic acid content, the acid-base value and the environmental temperature of the sweat and correspondingly generating lactic acid content information, acid-base value information and environmental temperature information, the lactic acid sensor comprises a working electrode and a reference electrode, and a gold layer is arranged on the surface of the working electrode;
and the processing module is used for correcting the lactic acid content information according to the pH value information and the environmental temperature information so as to obtain lactic acid content data of the sweat.
2. The sweat lactate detection device of claim 1, wherein gold nano-pine needles are deposited on the surface of the working electrode.
3. The sweat lactate detection device of claim 1, wherein the lactate sensor further comprises lactate oxidase and a crosslinker, the lactate oxidase being covalently crosslinked to the working electrode and the reference electrode by the crosslinker.
4. The sweat lactate detection device of claim 3, wherein the cross-linking agent is polyethylene glycol diglycidyl ether.
5. The sweat lactate detection device of claim 1, wherein the collection module includes a hydrophobic membrane having a hollow structure forming a detection chamber and a plurality of absorption tubes spaced around the detection chamber and respectively communicating with the detection chamber, the absorption tubes being configured to channel the sweat to the detection chamber.
6. A sweat lactate detection method for use with a sweat lactate detection device as claimed in any one of claims 1-5, said sweat lactate detection method comprising the steps of:
collecting sweat;
collecting the lactic acid content, the pH value and the environmental temperature of the sweat and correspondingly generating lactic acid content information, pH value information and environmental temperature information, wherein the lactic acid content of the sweat is collected through a lactic acid sensor, the lactic acid sensor comprises a working electrode and a reference electrode, and a gold layer is arranged on the surface of the working electrode;
filtering and amplifying the lactic acid content information, the pH value information and the environmental temperature information;
and correcting the lactic acid content information according to the pH value information and the environmental temperature information to obtain lactic acid content data of the sweat.
7. The sweat lactate detection method of claim 6, wherein the working electrode and the reference electrode are both fabricated using electron beam evaporation plating processes.
8. The sweat lactate testing device of claim 7 wherein the working electrode is fabricated using an electron beam evaporation coating process comprising the steps of:
providing a first flexible electrode;
electroplating a titanium layer with the thickness of 4nm to 6nm on the surface of the first flexible electrode;
electroplating a gold layer with the thickness of 55nm to 65nm on the surface of the titanium layer to obtain the working electrode;
the reference electrode is prepared by adopting an electron beam evaporation coating process, and comprises the following steps:
providing a second flexible electrode;
electroplating a titanium layer with the thickness of 4nm to 6nm on the surface of the second flexible electrode;
electroplating a silver layer with the thickness of 190nm to 210nm on the surface of the titanium layer;
soaking the second flexible electrode in a 3M potassium chloride solution;
and carrying out chlorination treatment on the second flexible electrode by a chronopotentiometric process to prepare the reference electrode.
9. The sweat lactate testing device of claim 6, wherein said modifying the lactate content information based on the pH information and ambient temperature information to obtain lactate content data for the sweat comprises:
and calculating to obtain a corrected real lactic acid content value according to a formula L (C-Kt T + C0)/(ph Kp + K0), wherein L is the corrected real lactic acid content value, C is a current measured value of the lactic acid sensor, Kt is the sensitivity (slope) of the current measured value of the lactic acid sensor along with the change of temperature, T is the body temperature, C0 is the current measured initial value of the lactic acid sensor, ph is the current measured value of the pH value sensor, Kp is the slope of the sensitivity of the current measured value of the lactic acid sensor along with the change of the real lactic acid value along with the change of the ph value, and K0 is the current change sensitivity initial value of the lactic acid sensor.
10. The sweat lactate detection device of claim 8, wherein a gold layer is electroplated to a thickness of 55nm to 65nm on the surface of the titanium layer to produce the working electrode, and then further comprising:
soaking the working electrode in a sodium chloride solution with the concentration of the tetrachloroauric acid between 2.5mg/mL and 3.5 mg/mL;
and carrying out deposition treatment on the working electrode by an electrochemical deposition process so as to deposit and form the gold nano pine needles on the surface of the working electrode.
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