CN114324318A - Portable pesticide residue detection device and method - Google Patents
Portable pesticide residue detection device and method Download PDFInfo
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- CN114324318A CN114324318A CN202210245540.0A CN202210245540A CN114324318A CN 114324318 A CN114324318 A CN 114324318A CN 202210245540 A CN202210245540 A CN 202210245540A CN 114324318 A CN114324318 A CN 114324318A
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Abstract
The invention relates to a portable pesticide residue detection device and a method, which are used for solving the problem that the existing pesticide monitoring device is not easy to carry. The portable pesticide residue detection device of the invention comprises: the base is provided with a rotating table and a sample placing part; the fixed seat is arranged on the rotating table and can horizontally rotate relative to the rotating table; the detection assemblies are detachably mounted on the fixed seat and synchronously rotate along with the fixed seat; the detection assembly is provided with a liquid storage cavity for storing a reagent for detecting pesticide residues; the detection assembly is provided with a liquid outlet communicated with the liquid storage cavity, the detection assembly rotates for a circle along with the fixing seat, and the liquid outlet passes through the upper part of the sample placing part at least once, so that the reagent can be dripped to the sample placed on the sample placing part.
Description
Technical Field
The invention relates to the technical field of food safety detection, in particular to a portable pesticide residue detection device and method.
Background
Organophosphorus and carbamate pesticides have been widely used in the production of agricultural products such as fruits and vegetables, but the long-term consumption of agricultural products containing organophosphorus and carbamate pesticide residues has serious harm to human health. Most of the existing detection technologies need large-scale instruments and professional staff to participate, and portable detection cannot be realized.
Disclosure of Invention
In view of the above analysis, the present invention provides a portable pesticide residue detection device and method, so as to solve the problem that the conventional pesticide monitoring device is not easy to carry.
In one aspect, the present invention provides a portable pesticide residue detection apparatus, comprising:
the base is provided with a rotating table and a sample placing part;
the fixed seat is arranged on the rotating table and can horizontally rotate relative to the rotating table;
the detection assemblies are detachably mounted on the fixed seat and synchronously rotate along with the fixed seat; the detection assembly is provided with a liquid storage cavity for storing a reagent for detecting pesticide residues; the detection assembly is provided with a liquid outlet communicated with the liquid storage cavity, the detection assembly rotates for a circle along with the fixing seat, and the liquid outlet passes through the upper part of the sample placing part at least once, so that the reagent can be dripped to the sample placed on the sample placing part.
Furthermore, the detection assembly is arranged on the fixed seat in a centrosymmetric and detachable manner, and the central symmetric axis is a rotating axis of the fixed seat;
the detection assembly comprises an injector and an operating mechanism, and the operating mechanism drives a piston push rod of the injector to move up and down; and the base is provided with a scale gear meshed with the control gear of the operating mechanism.
Furthermore, the fixed seat is provided with an assembling part matched with the detection assembly;
the assembly portion be equipped with the same fixed chamber of detecting component quantity, the one end that detecting component was equipped with the liquid outlet passes fixed chamber is located the below of assembly portion, detecting component's the other end is located the top of assembly portion or be located fixed intracavity.
Furthermore, the injector comprises a sleeve and a piston push rod, the piston push rod is inserted into the sleeve and limited to be out of the liquid storage cavity, and the bottom of the sleeve is provided with the liquid outlet;
the upper end of the sleeve and the upper part of the piston push rod are detachably arranged in the operating mechanism.
Further, the operating mechanism comprises an operating shell, a worm, a moving piece and a control gear;
the operation shell is provided with an operation cavity and comprises a shell body and a side cover plate which are detachably connected, and one side of the shell body is provided with an opening; the side cover plate is detachably covered on the opening and is limited out of the operation cavity together with the shell; the side cover plate comprises a lapping part arranged at the top, and a space for the control gear to horizontally rotate is limited by the lapping part and the top wall of the shell;
the moving piece is positioned in the operation cavity and matched with the side wall of the operation cavity, so that the moving piece can move up and down in the operation cavity along the side wall of the operation cavity;
a screw hole is formed in the center of the control gear, threads of the screw hole are matched with grains of the worm, and the control gear is located between the lap joint part and the top wall of the shell;
the bottom end of the worm penetrates through the top wall of the shell and extends into the operation cavity to be connected with the moving piece, and the top end of the worm sequentially penetrates through a screw hole and a lap joint part of the control gear;
the top end of the sleeve is detachably mounted in the shell, and the top end of the piston push rod is detachably connected with the moving piece;
and when the control gear is rotated, the worm moves upwards or downwards, the moving piece moves synchronously along with the worm, and the piston push rod moves synchronously along with the moving piece.
Further, the bottom of fixing base is equipped with spacing portion, spacing portion is equipped with spacing hole that detecting component quantity is the same, spacing hole with detecting component's bottom cooperatees.
Furthermore, the base is also provided with a support column, the support column and the rotating platform are oppositely arranged on two sides of the sample placing part, the top end of the support column is provided with a scale gear, the height of the scale gear is the same as that of the control gear, and the scale gear can be meshed with the control gear;
the upper end face of the scale gear is provided with an annular scale and a pointer, the center of the annular scale is the circle center of the scale gear, the pointer is positioned at the center of the annular scale, and the pointer is static relative to the pillar;
the pillar with detection component matched with position is equipped with first magnetism respectively and inhales portion and second magnetism and inhales the portion, the fixing base with revolving stage matched with position is equipped with third magnetism respectively and inhales portion and fourth magnetism and inhales the portion.
Further, the portable pesticide residue detection device also comprises a sample plate, wherein the sample plate is provided with a plurality of sample grooves;
the sample placing part is provided with a sliding groove matched with the sample plate so that the sample plate can slide in the sample placing part.
Further, the length, the width and the height of an external cube of the portable pesticide residue detection device are both less than 20 cm;
the base, the supporting column, the rotating platform, the fixing seat, the sample plate and the operating mechanism of the portable pesticide residue detection device are manufactured by adopting a 3D printing technology.
In another aspect, the present invention provides a method for detecting pesticide residue by using the portable pesticide residue detection device as claimed in the above claims, comprising the steps of:
the method comprises the following steps: manufacturing sample test paper of different pesticide residue articles to be detected, respectively placing the sample test paper into sample grooves of a sample plate, and then placing the sample plate into a sample placing part;
adding acetylcholinesterase, acetylcholine, TMB and 30% H into the liquid storage cavities of different detection assemblies respectively2O2Four solutions;
step two: sequentially dropwise adding 18-22 mu L of the acetylcholinesterase solution to the sample test paper;
step three: after 14-16 min, continuously dropwise adding 18-22 mu L of the acetylcholine solution to the sample test paper in sequence;
step four: after 9-11 min, continuously dropwise adding 9-11 mu L of TMB solution and 9-11 mu L of H to the sample test paper in sequence2O2A solution;
step five: and after 4-6 min, judging the pesticide residue through the color of the sample after the reaction of the test paper.
Compared with the prior art, the invention can realize at least one of the following beneficial effects:
(1) the detection assemblies which are detachably mounted are integrated on the fixed seat which can rotate horizontally, so that not only is the compactness of the device improved, but also the operability of the device is improved;
(2) the liquid output amount of the injector is controlled through the operating mechanism, so that the volume of the micro fluid can be accurately controlled, and the detection precision of the device is improved; when the injector is replaced, the injector can be taken out only by opening the side cover plate of the operating shell, so that the device is more convenient to assemble and maintain;
(3) the scale gear can be shared by a plurality of detection assemblies, so that the utilization rate of the scale gear is improved, the volume of the device is saved, and the structural compactness of the device is improved;
(4) the sample plate is provided with a plurality of sample grooves, so that a plurality of samples or sample test paper can be detected simultaneously, and the operating efficiency is improved;
(5) most parts of the device are manufactured by adopting a 3D printing technology, so that the device has the advantages of small volume, light weight, low cost, portability and the like;
(6) based on the device, a complete on-site accurate, efficient and portable pesticide residue detection method can be constructed.
In the invention, the technical schemes can be combined with each other to realize more preferable combination schemes. Additional features and advantages of the invention will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. The objectives and other advantages of the invention will be realized and attained by the structure particularly pointed out in the written description and drawings.
Drawings
The drawings are only for purposes of illustrating particular embodiments and are not to be construed as limiting the invention, wherein like reference numerals are used to designate like parts throughout.
FIG. 1 is a schematic structural diagram of a portable pesticide residue detection device according to an embodiment;
FIG. 2 is a schematic structural diagram of a portable pesticide residue detection device according to an embodiment (II);
FIG. 3 is a schematic structural diagram (I) of the portable pesticide residue detection device after all detection components are taken out;
FIG. 4 is a schematic structural diagram of the portable pesticide residue detection apparatus with all detection components removed according to the embodiment (II);
FIG. 5 is a schematic diagram of the structure of a base (with a support post and a turntable) in an embodiment;
FIG. 6 is a schematic structural diagram of a fixing base according to an embodiment;
FIG. 7 is a schematic structural diagram of a fixing base in the embodiment (II);
FIG. 8 is a schematic structural view of an operating mechanism in accordance with an embodiment;
FIG. 9 is a schematic structural view of an operating shell in accordance with an embodiment;
fig. 10 is a sectional view (one) of a portable pesticide residue detection apparatus in an embodiment;
fig. 11 is a sectional view of the portable pesticide residue detection apparatus according to the embodiment (ii).
Reference numerals:
1-a base; 11-a rotating table; 111-fourth magnetic attraction; 12-a sample placement section; 13-a pillar; 131-a scale gear; 131 a-annular scale; 131 b-a pointer; 132-a first magnetic attraction; 2-a fixed seat; 21-an assembly part; 201-a fixed cavity; 201 a-a through hole; 201 b-a fixation slot; 22-a limiting part; 221-limiting hole; 23-a third magnetic attraction part; 3-a detection component; 31-a syringe; 311-a sleeve; 312-piston pusher rod; 313-injection needle; 32-an operating mechanism; 321-a shell; 321 a-open mouth; 321 b-a first card slot; 321 c-a first perforation; 321 d-a second perforation; 322-side cover plate; 322 a-lap joint; 322 b-port; 323-worm; 324-a mover; 324 a-a second card slot; 324 b-a third perforation; 325-control gear; 326-visible window; 33-a second magnetic attraction part; 4-sample plate; 41-sample tank.
Detailed Description
The preferred embodiments of the present invention will now be described in detail with reference to the accompanying drawings, which form a part hereof, and which together with the embodiments of the invention serve to explain the principles of the invention and not to limit its scope.
In the description of the embodiments of the present invention, it should be noted that, unless otherwise explicitly stated or limited, the term "connected" should be interpreted broadly, and may be, for example, a fixed connection, a detachable connection, or an integral connection, which may be a mechanical connection, an electrical connection, which may be a direct connection, or an indirect connection via an intermediate medium. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
The terms "top," "bottom," "above … …," "below," and "on … …" as used throughout the description are relative positions with respect to components of the device, such as the relative positions of the top and bottom substrates inside the device. It will be appreciated that the devices are multifunctional, regardless of their orientation in space.
The general working surface of the invention can be a plane or a curved surface, can be inclined or horizontal. For convenience of explanation, the embodiments of the present invention are placed on a horizontal plane and used on the horizontal plane, and are defined as "high and low" and "up and down".
Example one
The embodiment discloses a portable pesticide residue detection device, as shown in fig. 1-11, includes:
a base 1 provided with a turntable 11 and a sample placing section 12;
the fixed seat 2 is arranged on the rotating table 11 and can horizontally rotate relative to the rotating table 11;
the detection assembly 3 is detachably mounted on the fixed seat 2 and synchronously rotates along with the fixed seat 2; the detection assembly 3 is provided with a liquid storage cavity for storing a reagent for detecting pesticide residues; the detection assembly 3 is provided with a liquid outlet communicated with the liquid storage cavity, the detection assembly 3 rotates for a circle along with the fixing seat 2, and the liquid outlet passes through the sample placing part 12 at least once from the upper part so as to be prepared for dripping the reagent to the sample placed in the sample placing part 12.
Preferably, portable pesticide residue detection device includes a plurality of determine module 3, a plurality of determine module 3 use the rotation axis of fixing base 2 as the center evenly install in on the fixing base 2, every determine module 3 equals to the distance of the 2 rotation axes of fixing base promptly, and all determine module 3 are central symmetry demountable installation on fixing base 2, and central symmetry axis is the rotation axis of fixing base 2. So set up to guarantee fixing base 2's stability in rotation, and improve determine module 3's operating stability.
In this embodiment, portable pesticide residue detection device includes four determine module 3, and four determine module 3 use the rotation axis of fixing base 2 as the even demountable installation of center on fixing base 2, and four determine module 3 adopt "ten" word to distribute on fixing base 2 promptly, and four determine module 3 are located four ends of "ten" word.
Be equipped with on fixing base 2 with the matched with assembly portion 21 of determine module 3, determine module 3 through assembly portion 21 demountable installation on fixing base 2. Specifically, the assembly portion 21 is provided with the fixed cavity 201 the same with the detection assembly 3 quantity, the fixed cavity 201 is provided with the assembly channel that can run through the fixed cavity from top to bottom, it is penetrating about the fixed cavity 201 promptly, the one end that the detection assembly 3 is equipped with the liquid outlet passes fixed cavity 201 and is located the below of assembly portion 21, the other end of detection assembly 3 is located the top of assembly portion 21 or is located fixed cavity 201, detection assembly 3 is through the mode demountable installation of pegging graft on the fixed cavity 201 of assembly portion 21.
In some embodiments, the liquid outlet of the detecting assembly 3 is provided with a switch, and the liquid outlet is operatively opened or closed by the switch, that is, the liquid outlet is controlled by the switch to control the liquid outlet amount of the reagent, and preferably, the switch is a quantitative switch, so as to determine the accurate outflow amount of the reagent and improve the accuracy of pesticide residue detection. The switch is located below the mounting portion 21.
In this embodiment, the detection assembly 3 includes the syringe 31, and the syringe 31 is equipped with stock solution chamber and liquid outlet, and the syringe 31 includes sleeve 311 and piston push rod 312, and the piston push rod 312 inserts and restricts in the sleeve 311 and goes out the stock solution chamber, and the bottom of sleeve 311 is equipped with the liquid outlet. The piston push rod 312 is pushed, the reagent in the liquid storage cavity flows out from the liquid outlet, and the volume of the liquid storage cavity changes along with the movement of the piston push rod 312.
In order to conveniently control the liquid outlet amount, the sleeve 311 is made of transparent material, and the side wall of the sleeve 311 is provided with scales; an injection needle 313 is arranged at the liquid outlet at the bottom of the sleeve 311 to control the liquid outlet amount of the reagent more stably.
In order to more accurately control the amount of fluid discharged from syringe 31 and to more easily operate syringe 31. The detection assembly 3 further comprises an operating mechanism 32, a portion of the syringe 31 being detachably mounted in said operating mechanism 32, in particular at least the upper end of the sleeve 311 and the upper portion of the piston pusher 312 being detachably mounted in the operating mechanism 32.
Specifically, the operating mechanism 32 includes an operating case, a worm 323, a moving member 324, and a control gear 325.
The operation shell is provided with an operation cavity, the operation shell comprises a shell body 321 and a side cover plate 322 which are detachably connected, an opening 321a is formed in one side of the shell body 321, the side cover plate 322 is detachably covered on the opening 321a and is limited out of the operation cavity together with the shell body 321, and the operation cavity is opened or closed from the side edge of the operation shell through the side cover plate 322 in an operable mode. The side cover 322 includes a lap portion 322a formed at the top thereof, and the lap portion 322a and the top wall of the housing 321 define a space for the control gear 325 to horizontally rotate. Preferably, a central axis of the operating case passes through the overlapping portion 322a, and the central axis of the operating case is parallel to the rotation axis of the fixing base 2.
The moving member 324 is located in the operation cavity, and the side wall of the moving member 323 is matched with the side wall of the operation cavity, so that the moving member 324 can move up and down in the operation cavity along the side wall of the operation cavity.
The control gear 325 is provided with a screw hole at the center, and the thread of the screw hole is matched with the grains of the worm 323 so as to control the worm 323 to move up and down by rotating the control gear 325. The bottom end of the worm 323 penetrates through the top wall of the shell 321 to extend into the operation cavity to be connected with the moving member 324, the top end of the worm 323 sequentially penetrates through a screw hole of the control gear 325 and the overlapping part 322a of the side cover plate 322, and the control gear 325 is limited between the overlapping part 322a of the side cover plate 322 and the top wall of the shell 321. When the control gear 325 is rotated, the worm 323 moves up or down, and the moving member 324 moves up or down along with the worm 322. Preferably, the central axis of the worm 323 is collinear with the central axis of the operating case.
It should be noted that the overlapping portion 322a is located above the top wall of the housing 321, the overlapping portion 322a is provided with a through hole 322b for the top end of the worm 323 to pass through, and the through hole 322b extends and opens outward (the outward direction is away from the direction of the opening) toward the overlapping portion 322a, so as to prevent the worm 323 from affecting the detachment of the side cover 322. The length of the worm 323 is at least greater than the sum of the height of the operating chamber and the thickness of the operating gear 325 to avoid the worm 323 falling out of the threaded hole into the operating chamber. To facilitate rotation of the control gear 325, the operating housing is disposed on one side of the side cover plate 322 near the central axis of the fixing base 2.
The sleeve 311 is detachably fixed at least at the top end to the housing 321; the top end of the piston push rod 312 is detachably connected with the moving member 324, and at least part of the movable push rod 312 is located in the operation cavity and synchronously moves along with the moving member 324, so that the pushing of the movable push rod 312 is controlled by rotating the control gear 325, and the liquid output of the injector is further controlled.
Specifically, the bottom of the housing 321 is provided with a first locking groove 321b matched with the top end of the sleeve 311, and a notch of the first locking groove 321b is flush with the opening 321 a. The top wall of the first engaging groove 321b is provided with a first through hole 321c for the piston rod 312 to pass through and move, the bottom wall of the first engaging groove 321b is provided with a second through hole 321d for the sleeve 311 to pass through, and the first through hole 321c and the second through hole 321d extend toward the notch of the first engaging groove 321b and are communicated with the notch of the first engaging groove 321 b. The moving member 324 is provided with a second engaging groove 324a engaged with the top end of the piston rod 312, a notch of the second engaging groove 324a faces the direction of the opening 321a, a bottom wall of the second engaging groove 324a is provided with a third through hole 324b for the piston rod 312 to pass through, and the third through hole 324b extends toward the notch of the second engaging groove 324a and is communicated with the notch of the second engaging groove 324 a.
Process for detachable mounting of syringe 31 to operating mechanism 32: the side cover plate 322 is taken out from the housing 321, the top end of the piston push rod 312 and the top end of the sleeve 311 are aligned with the second locking groove 324a and the first locking groove 321b respectively to push the syringe inwards, the top end of the piston push rod 312 is locked into the moving member 324, the sleeve 311 is locked into the bottom end of the housing 321, and the side cover plate 322 is covered on the housing 321, so that the syringe 31 is assembled into the operating mechanism 32. It should be noted that, when the top end of the piston push rod 312 is not aligned with the second engaging groove 324a, the control gear 325 is rotated to drive the worm 323 to adjust the position of the moving member 324, so that the second engaging groove 324a is aligned with the top end of the piston push rod 312.
In this embodiment, the fixing cavity 201 of the assembling portion 21 is matched with the operating mechanism 32, specifically, the bottom of the fixing cavity 201 is provided with a through hole 201a for the sleeve 311 to pass through and a fixing groove 201b communicated with the through hole 201a, and the fixing groove 201b is matched with the bottom of the operating shell, that is, the bottom of the operating shell is clamped into the fixing groove 201 b. It should be noted that the notch of the fixing groove 201b is vertically upward, the fixing groove 201b is provided with a through hole 201a, and the fixing groove 201b and the through hole 201a together form an assembly channel for the bottom of the detecting assembly 3 to pass through.
In order to facilitate observation of the moving condition of the moving member 324 in the operation cavity, the casing 321 is provided with a viewing window 326 opposite to the side cover plate 322, and the viewing window 326 may be a hollow structure or a transparent material, as long as the condition in the operation cavity is not affected.
In order to improve the assembly stability of the detection assembly 3 and improve the operation stability of the portable pesticide residue detection device, the bottom of the fixing seat 2 is provided with a limiting part 22, the limiting part 22 is provided with limiting holes 221 with the same number of the detection assemblies 3, the limiting holes 221 are matched with the bottom end of the detection assembly 3, and the limiting holes 221 are in one-to-one correspondence with the fixing cavities 201. In this embodiment, the shape of the limiting portion 22 is similar to "ten", four ends of the "ten" are respectively provided with a limiting hole 221 matched with the bottom end of the syringe 31, specifically, the injection needle 313 penetrates through the limiting hole 221, the bottom end of the sleeve 311 is located in the limiting hole 221, and the limiting hole 221 is located right below the fixing cavity 201.
In order to conveniently and quickly control the liquid discharge amount of each detection assembly 3, the base 1 is further provided with a support column 13, the support column 13 and the rotating table 11 are oppositely arranged at two sides of the sample placing part 12, the top end of the support column 13 is provided with a scale gear 131, the height of the scale gear 131 is the same as that of the control gear 325, the scale gear 131 can be meshed with the control gear 325, and the upper end surface of the scale gear 131 is provided with an annular scale 131a and a pointer 131b, so that the liquid discharge amount of the syringe 31 is controlled by rotating the scale gear 131, that is, the scale gear 131 is rotated, the control gear 325 meshed with the scale gear 131 rotates along with the rotation, and then the worm 323, the moving member 324 and the piston push rod 312 synchronously move, and further the liquid discharge amount of the syringe 31 is controlled.
Specifically, the center of the annular scale 131a is the center of the scale gear, the pointer 131b is located at the center of the annular scale 131a, and the pointer 131b passes through the scale gear 131 and is connected with the pillar 13, i.e. the pointer 131b does not rotate with the scale gear 131, i.e. the pointer 131b is stationary relative to the pillar 13.
In this embodiment, the range of the syringe is 100 μ L, and the range of the scale gear 131 is 150 μ L, i.e. after the control gear 325 is engaged with the scale gear 131, the scale gear 131 rotates one turn (i.e. rotates 360 °), and the liquid discharge amount is 150 μ L.
Note that the control gears 325 of the plurality of detection units 3 are sequentially engaged with the scale gear 131 by rotating the fixing base 2. When the scale gear 131 is engaged with the control gear 325 of a certain detection unit 3, the liquid outlet (or the injection needle 313) of the detection unit 3 is positioned directly above the sample placement unit 12.
Preferably, the length, width and height of the external cube of the portable pesticide residue detection device (i.e. the external cube after the assembly of the rear base, the pillar, the detection assembly and the fixing seat is completed) are all less than 20cm, that is, the length, width and height of all the components of the portable pesticide residue detection device are all less than 20cm for convenient carrying, in this embodiment, the length, width and height of the external cube are 10cm, 11cm and 20cm respectively.
In order to detect a plurality of samples simultaneously, the portable pesticide residue detection device further comprises a sample plate 4, wherein the sample plate 4 is provided with a plurality of sample grooves 41, and the sample grooves 41 are used for placing samples or sample test paper. Further, the sample placing section 12 is provided with a slide groove that is matched with the sample plate 4, so that the sample plate 4 can slide in the sample placing section 12, so that the plurality of sample grooves 41 are located below the liquid outlet in sequence, and the reagent is dropped into the sample grooves 41.
In order to further improve the detection speed of the portable pesticide residue detection device, the positions of the pillar 13 and the detection component 3 are respectively provided with a first magnetic attraction part 132 and a second magnetic attraction part 33, the positions of the fixed seat 2 and the rotating platform 11 are respectively provided with a third magnetic attraction part 23 and a fourth magnetic attraction part 111, specifically, the side wall of the pillar 13 opposite to the fixed seat 2 is provided with the first magnetic attraction part 132, the position of the outer side wall of each detection component 3 and the first magnetic attraction part 132 is provided with the second magnetic attraction part 33, the side wall of the rotating platform 11 far away from the pillar 13 is provided with the fourth magnetic attraction part 111, that is, the fourth magnetic attraction parts 111 and the first magnetic attraction parts 132 are distributed diagonally, the fixing base 2 is provided with the same number of third magnetic attraction parts 23 as the number of the detecting elements 3, the distribution of the third magnetic attraction parts 23 is related to the distribution of the detecting assembly 3, and the third magnetic attraction parts 23 are matched with the fourth magnetic attraction parts 111. In this embodiment, the second magnetic part 33 is located on the outer sidewall of the housing 321 away from the side cover 322, and the third magnetic part 23 is located at the bottom of the position-limiting part 22 and is adjacent to the position-limiting hole 221.
When the scale gear 131 meshes with the control gear 325 of a certain detection component 3, the first magnetic attraction part 132 and the second magnetic attraction part 33 of the detection component 3 are magnetically attracted together, the fourth magnetic attraction part 111 and the third magnetic attraction part 23 below the detection component 3 are magnetically attracted together, and the two magnetic attraction parts are distributed diagonally, so that the control gear 325 of the detection component 3 can be rapidly meshed with the scale gear 131, and the operation stability of the portable pesticide residue detection device can be further improved.
Preferably, most of the components of the portable pesticide residue detection device are manufactured by using a 3D printing technology, for example, the base 1 (including the support column 13 and the rotating table 11), the fixing base 2, the sample plate 4, the operating shell and the like are manufactured by using a 3D printing technology, which has the advantages of light weight and low cost, and the total weight of the whole portable pesticide residue detection device is not more than 10 g. In this embodiment, the base, the fixing base, and the sample plate are made of a polyacetic acid material by an FDM-3D printer, and the total weight of the portable pesticide residue detection apparatus is 8.25 g.
Preferably, the syringe 31 is a micro-syringe.
Preferably, each test element 3 is equipped with a different reagent, the reagent in each test element 3 being selected according to the type of pesticide residue to be tested.
The detection device of the present invention is not limited to the detection of pesticide residues, and may be used in other fields where reagent detection is employed.
Example two
The embodiment discloses a pesticide residue detection method, which adopts the portable pesticide residue detection device provided by the embodiment I to detect, and comprises the following steps:
the method comprises the following steps: manufacturing sample test paper of different pesticide residue articles to be detected, respectively placing the sample test paper into sample grooves of a sample plate, and then placing the sample plate into a sample placing part;
adding Acetylcholinesterase (AChE), Acetylcholine (ACethylcholine, ACh), 3',5,5' -Tetramethylbenzidine (TMB) and 30% H into the liquid storage cavities of different detection assemblies respectively2O2(hydrogen peroxide) four solutions;
step two: sequentially dropwise adding 18-22 mu L of the acetylcholinesterase solution to the sample test paper;
step three: after 14-16 min, continuously dropwise adding 18-22 mu L of the acetylcholine solution to the sample test paper in sequence;
step four: after 9-11 min, continuously dropwise adding 9-11 mu L of TMB solution and 9-11 mu L of H to the sample test paper in sequence2O2A solution;
step five: and after 4-6 min, judging the pesticide residue through the color of the sample after the reaction of the test paper.
The blank test paper of the sample test paper adopts monoatomic nanoenzyme bioactive paper, the paper-based carrier of the blank test paper is Wottman 1 paper, and the blank test paper is soaked in the Wottman 1 paper to simulate the activity of peroxidase (the activity of the natural peroxidase can be simulated, and the activity of the natural peroxidase can be simulated in H)2O2In the presence of (C) a catalyst H2O2Generating hydroxyl radical to oxidize substrate such as TMB), and drying at 37 deg.C.
The monoatomic cerium nanoenzyme solution is obtained by dissolving monoatomic cerium nanoparticles in a system of 99% ethanol and 1% 5% Nafion solution (containing 5% perfluorosulfonic acid polymer solution) at a concentration of 0.08-0.10 [ mu ] mol/L (the concentration of the monoatomic cerium nanoenzyme in the mixed solvent of ethanol and Nafion) and performing ultrasonic treatment.
The principle design of pesticide residue detection: the product Choline (Choline, Ch) generated by the reaction of AChE and ACh has certain reducing capability and can oxidize SACe-N-C nano enzyme into blue oxidation state TMB (TMB)ox) Reduced to colorless reduced TMB (TMB)red) So that the blue color of the system becomes lighter. The organophosphorus and carbamate pesticides can destroy the active center of the esterification part of AChE, so that AChE generates phosphorylated acetylcholinesterase and carbamylated acetylcholinesterase, the activity of AChE disappears, less or even no Ch is generated, and the system can normally generate blue TMBox。
[ example ] step one: washing the surface of the articles to be detected with pesticide residues, such as broccoli, ginger, rape, celery, pakchoi, Chinese cabbage, apple and tomato, with distilled water, and collecting the washing solution as the detection solution. Respectively dripping 10 mu L of detection liquid onto the monoatomic cerium bioactive paper to obtain sample test paper, putting the sample test paper of different fruits and vegetables into a sample groove of a sample plate, and putting the sample plate into a sample placing part;
adding different liquid storage cavities into the portable pesticide residue detection device respectively1mL of 25mmol/L AChE solution, 1mL of 5mmol/L Ach solution, 1mL of 1mmol/L TMB solution, and 1mL of 0.625 mmol/L30% H2O2And (3) solution.
Step two: rotating the detection assembly containing the AChE solution to a scale gear, enabling a control gear of the detection assembly containing the AChE solution to be meshed with the scale gear, and rotating the scale gear to sequentially dropwise add 20 mu L of AChE solution to different sample test paper;
organic phosphorus (
) And carbamates, (C)
) When present, active AChE is inactivated and loses the activity of breaking down Ach. This process takes 15 min.
Step three: after waiting for 15min, rotating the detection assembly containing the ACh solution to a scale gear, meshing a control gear of the detection assembly containing the ACh solution with the scale gear, and rotating the scale gear to sequentially dropwise add 20 mu L of ACh solution to different sample test paper;
inactivated AChE, which does not decompose Ach to produce Chox and acetate.
Step four: after waiting for 10min, rotating the detection assembly with the TMB solution to a scale gear, meshing a control gear of the detection assembly with the TMB solution with the scale gear, and rotating the scale gear to sequentially dropwise add 10 mu L of TMB solution to different sample test paper; will then put H2O2The detection component of the solution rotates to the position of the scale gear, so that H is placed in the detection component2O2The control gear of the solution detection assembly is meshed with the scale gear, and the scale gear is rotated to different directions10 mu L of H is sequentially dripped into the sample test paper2O2And (3) solution.
SACe-N-C has peroxidase-mimicking activity, and will be in H2O2In the presence of (2) adding a TMBredBy oxidation to TMBoxThe system turns blue and produces H2O。
And if ChoxWill have TMB when presentoxAnd is reduced to TMBredChange itself to ChredAnd the system color is restored to blue.
Step five: after all solutions are dripped, the color change of the sample test paper is observed for 5min, the field can be fast through the depth of the color, the content of pesticide residues in the sample is conveniently detected, and the deeper the color is, the higher the residue is. And finally, measuring the gray level through Image J software (photographing the reacted color, uploading the photographed color to the Image J software, and directly selecting a gray level analysis program), thereby realizing quantitative detection.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are included in the scope of the present invention.
Claims (10)
1. A portable pesticide residue detection device, comprising:
the base is provided with a rotating table and a sample placing part;
the fixed seat is arranged on the rotating table and can horizontally rotate relative to the rotating table;
the detection assemblies are detachably mounted on the fixed seat and synchronously rotate along with the fixed seat; the detection assembly is provided with a liquid storage cavity for storing a reagent for detecting pesticide residues; the detection assembly is provided with a liquid outlet communicated with the liquid storage cavity, the detection assembly rotates for a circle along with the fixing seat, and the liquid outlet passes through the upper part of the sample placing part at least once, so that the reagent can be dripped to the sample placed on the sample placing part.
2. The portable pesticide residue detection device of claim 1, wherein the detection assembly is centrally symmetrical and detachably mounted on the fixed base, and the central symmetry axis is a rotation axis of the fixed base;
the detection assembly comprises an injector and an operating mechanism, and the operating mechanism drives a piston push rod of the injector to move up and down; and the base is provided with a scale gear meshed with the control gear of the operating mechanism.
3. The portable pesticide residue detection device of claim 2, wherein the fixed seat is provided with an assembling part matched with the detection assembly;
the assembly portion be equipped with the same fixed chamber of detecting component quantity, the one end that detecting component was equipped with the liquid outlet passes fixed chamber is located the below of assembly portion, detecting component's the other end is located the top of assembly portion or be located fixed intracavity.
4. The portable pesticide residue detection device of claim 3, wherein the injector comprises a sleeve and a piston push rod, the piston push rod is inserted into the sleeve to limit the position of the piston push rod out of the liquid storage cavity, and the bottom of the sleeve is provided with the liquid outlet;
the upper end of the sleeve and the upper part of the piston push rod are detachably arranged in the operating mechanism.
5. The portable pesticide residue detecting device as claimed in claim 4, wherein the operating mechanism comprises an operating case, a worm, a moving member and a control gear;
the operation shell is provided with an operation cavity and comprises a shell body and a side cover plate which are detachably connected, and one side of the shell body is provided with an opening; the side cover plate is detachably covered on the opening and is limited out of the operation cavity together with the shell; the side cover plate comprises a lapping part arranged at the top, and a space for the control gear to horizontally rotate is limited by the lapping part and the top wall of the shell;
the moving piece is positioned in the operation cavity and matched with the side wall of the operation cavity, so that the moving piece can move up and down in the operation cavity along the side wall of the operation cavity;
a screw hole is formed in the center of the control gear, threads of the screw hole are matched with grains of the worm, and the control gear is located between the lap joint part and the top wall of the shell;
the bottom end of the worm penetrates through the top wall of the shell and extends into the operation cavity to be connected with the moving piece, and the top end of the worm sequentially penetrates through a screw hole and a lap joint part of the control gear;
the top end of the sleeve is detachably mounted in the shell, and the top end of the piston push rod is detachably connected with the moving piece;
and when the control gear is rotated, the worm moves upwards or downwards, the moving piece moves synchronously along with the worm, and the piston push rod moves synchronously along with the moving piece.
6. The portable pesticide residue detection device of claim 5, wherein the bottom of the fixing seat is provided with a limiting part, the limiting part is provided with limiting holes with the same number of detection components, and the limiting holes are matched with the bottom ends of the detection components.
7. The portable pesticide residue detection device as claimed in claim 6, wherein the base is further provided with a support column, the support column is arranged on two sides of the sample placing part opposite to the rotating table, the top end of the support column is provided with a scale gear, the height of the scale gear is the same as that of the control gear, and the scale gear can be meshed with the control gear;
the upper end face of the scale gear is provided with an annular scale and a pointer, the center of the annular scale is the circle center of the scale gear, the pointer is positioned at the center of the annular scale, and the pointer is static relative to the pillar;
the pillar with detection component matched with position is equipped with first magnetism respectively and inhales portion and second magnetism and inhales the portion, the fixing base with revolving stage matched with position is equipped with third magnetism respectively and inhales portion and fourth magnetism and inhales the portion.
8. The portable pesticide residue detection apparatus as claimed in claim 7, further comprising a sample plate provided with a plurality of sample grooves;
the sample placing part is provided with a sliding groove matched with the sample plate so that the sample plate can slide in the sample placing part.
9. The portable pesticide residue detection device of any one of claims 1 to 8, wherein the length, width and height of the circumscribed cube of the portable pesticide residue detection device are all less than 20 cm;
the base, the supporting column, the rotating platform, the fixing seat, the sample plate and the operating mechanism of the portable pesticide residue detection device are manufactured by adopting a 3D printing technology.
10. A method for detecting a pesticide residue by using the portable pesticide residue detection device according to any one of claims 1 to 9, comprising the steps of:
the method comprises the following steps: manufacturing sample test paper of different pesticide residue articles to be detected, respectively placing the sample test paper into sample grooves of a sample plate, and then placing the sample plate into a sample placing part;
adding acetylcholinesterase, acetylcholine, TMB and 30% H into the liquid storage cavities of different detection assemblies respectively2O2Four solutions;
step two: sequentially dropwise adding 18-22 mu L of the acetylcholinesterase solution to the sample test paper;
step three: after 14-16 min, continuously dropwise adding 18-22 mu L of the acetylcholine solution to the sample test paper in sequence;
step four: after 9-11 min, continuously dropwise adding 9-11 mu L of TMB solution and 9-11 mu L of H to the sample test paper in sequence2O2A solution;
step five: and after 4-6 min, judging the pesticide residue through the color of the sample after the reaction of the test paper.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116026815A (en) * | 2022-07-21 | 2023-04-28 | 中国农业大学 | Halal meat food detection device and detection method thereof |
| CN116380886A (en) * | 2023-06-05 | 2023-07-04 | 中国农业大学 | Method for intelligently detecting organophosphorus pesticide by double signals |
| CN116859070A (en) * | 2023-09-01 | 2023-10-10 | 广东南国药业有限公司 | Sampling detection device and detection method for medicine production |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20130034466A1 (en) * | 2011-08-03 | 2013-02-07 | Yuji Wakamiya | Sample analyzer |
| CN206601338U (en) * | 2017-03-14 | 2017-10-31 | 山东省农业科学院农业质量标准与检测技术研究所 | Pesticide residue detection device |
| CN110398490A (en) * | 2019-08-20 | 2019-11-01 | 中国石油大学(华东) | Pesticide on-site detecting device and detection method |
| CN210005552U (en) * | 2019-05-31 | 2020-01-31 | 河南冠宇仪器有限公司 | novel multi-parameter food safety detector |
| CN112326646A (en) * | 2020-11-03 | 2021-02-05 | 童超 | Pesticide residue detection method for food detection |
| CN214794428U (en) * | 2021-06-08 | 2021-11-19 | 生态环境部南京环境科学研究所 | A portable pesticide detection device for pesticide short-term test |
-
2022
- 2022-03-14 CN CN202210245540.0A patent/CN114324318B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20130034466A1 (en) * | 2011-08-03 | 2013-02-07 | Yuji Wakamiya | Sample analyzer |
| CN206601338U (en) * | 2017-03-14 | 2017-10-31 | 山东省农业科学院农业质量标准与检测技术研究所 | Pesticide residue detection device |
| CN210005552U (en) * | 2019-05-31 | 2020-01-31 | 河南冠宇仪器有限公司 | novel multi-parameter food safety detector |
| CN110398490A (en) * | 2019-08-20 | 2019-11-01 | 中国石油大学(华东) | Pesticide on-site detecting device and detection method |
| CN112326646A (en) * | 2020-11-03 | 2021-02-05 | 童超 | Pesticide residue detection method for food detection |
| CN214794428U (en) * | 2021-06-08 | 2021-11-19 | 生态环境部南京环境科学研究所 | A portable pesticide detection device for pesticide short-term test |
Non-Patent Citations (1)
| Title |
|---|
| 姚田田: "比率型荧光检测有机磷农药的新方法研究", 《中国优秀博硕士学位论文全文数据库（硕士） 工程科技I辑》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116026815A (en) * | 2022-07-21 | 2023-04-28 | 中国农业大学 | Halal meat food detection device and detection method thereof |
| CN116380886A (en) * | 2023-06-05 | 2023-07-04 | 中国农业大学 | Method for intelligently detecting organophosphorus pesticide by double signals |
| CN116380886B (en) * | 2023-06-05 | 2023-08-15 | 中国农业大学 | Method for intelligently detecting organophosphorus pesticide by double signals |
| CN116859070A (en) * | 2023-09-01 | 2023-10-10 | 广东南国药业有限公司 | Sampling detection device and detection method for medicine production |
| CN116859070B (en) * | 2023-09-01 | 2023-11-07 | 广东南国药业有限公司 | Sampling detection device and detection method for medicine production |
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|---|---|
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