CN114323738B - Acquisition device for pig molecular genetic marker - Google Patents
Acquisition device for pig molecular genetic marker Download PDFInfo
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- CN114323738B CN114323738B CN202111553697.1A CN202111553697A CN114323738B CN 114323738 B CN114323738 B CN 114323738B CN 202111553697 A CN202111553697 A CN 202111553697A CN 114323738 B CN114323738 B CN 114323738B
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- 230000002068 genetic effect Effects 0.000 title claims abstract description 21
- 239000003550 marker Substances 0.000 title claims description 10
- 238000005070 sampling Methods 0.000 claims abstract description 30
- 230000000712 assembly Effects 0.000 claims description 9
- 238000000429 assembly Methods 0.000 claims description 9
- 230000001954 sterilising effect Effects 0.000 abstract description 14
- 108010010803 Gelatin Proteins 0.000 abstract description 11
- 150000001875 compounds Chemical class 0.000 abstract description 11
- 229920000159 gelatin Polymers 0.000 abstract description 11
- 239000008273 gelatin Substances 0.000 abstract description 11
- 235000019322 gelatine Nutrition 0.000 abstract description 11
- 235000011852 gelatine desserts Nutrition 0.000 abstract description 11
- 230000002439 hemostatic effect Effects 0.000 abstract description 11
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 abstract description 9
- 229910052740 iodine Inorganic materials 0.000 abstract description 9
- 239000011630 iodine Substances 0.000 abstract description 9
- 239000000645 desinfectant Substances 0.000 abstract description 7
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 abstract description 7
- 229920000036 polyvinylpyrrolidone Polymers 0.000 abstract description 7
- 239000007788 liquid Substances 0.000 description 16
- 241000282887 Suidae Species 0.000 description 11
- CPKVUHPKYQGHMW-UHFFFAOYSA-N 1-ethenylpyrrolidin-2-one;molecular iodine Chemical compound II.C=CN1CCCC1=O CPKVUHPKYQGHMW-UHFFFAOYSA-N 0.000 description 6
- 229920000153 Povidone-iodine Polymers 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 206010052428 Wound Diseases 0.000 description 6
- 208000027418 Wounds and injury Diseases 0.000 description 6
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 6
- 229960001621 povidone-iodine Drugs 0.000 description 6
- 210000005069 ears Anatomy 0.000 description 5
- 239000008280 blood Substances 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- 230000006978 adaptation Effects 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000000249 desinfective effect Effects 0.000 description 2
- 238000002649 immunization Methods 0.000 description 2
- 230000003053 immunization Effects 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 229920002635 polyurethane Polymers 0.000 description 2
- 239000004814 polyurethane Substances 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 108010070551 Meat Proteins Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000002255 vaccination Methods 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
Abstract
The invention relates to a collection device for pig molecular genetic markers, which comprises a sampling table and a blade accommodating groove, wherein a second sleeve is arranged above the sampling table, a longitudinally moving cutting mechanism is arranged in the second sleeve, the cutting mechanism comprises a second longitudinally moving component and a second sliding block, the second sliding block longitudinally moves through the second longitudinally moving component, a connecting block is arranged below the second sliding block, a cutting component is arranged at the lower end of the connecting block, the cutting component comprises an annular blade, and the annular blade and the blade accommodating groove are coaxially arranged. According to the invention, the detachable annular blade and the annular sponge body are arranged on the connecting block, the middle lower part of the annular sponge body is immersed with the polyvidone iodine sterilizing disinfectant, and the middle upper part of the annular sponge body is immersed with the gelatin compound hemostatic, so that the annular blade can be ensured to sterilize, disinfect and hemostatic the cut wound of the pig ear after sampling.
Description
Technical Field
The invention relates to the technical field of collection of genetic markers, in particular to a collection device for pig molecular genetic markers.
Background
Pigs, as an important human farm animal, provide a large amount of meat protein. In the large-scale raising of pigs, epidemic prevention work is important. The pigs raised artificially need to be injected with the corresponding vaccine according to the situation so as to obtain the corresponding immunity. In many places in china, after vaccinations of pigs, a piece of tissue is cut off from the ears of the pigs as a marker to indicate that the pigs have been vaccinated.
Further, in order to conduct further intensive studies on pigs, it is necessary to conduct studies, follow-up and analysis of the genetic and variability of pigs. The invention further utilizes a piece of tissue cut off from the ears of the pig during immunization of the pig, is used for genetic marker research of pig molecules, and can perform wound treatment on the place where the ears of the pig cut off the piece of tissue, so as to prevent infection and be beneficial to healthy growth of the pig.
Disclosure of Invention
The invention aims to solve the problems, and provides a collection device for pig molecular genetic markers, which is used for further utilizing a piece of tissue cut off from the ears of pigs during immunization of the pigs, is used for genetic marker research of pig molecules, can treat wounds at the places where the ears of the pigs cut off the piece of tissue, prevents infection, and can sterilize, disinfect and stanch the cut parts in time. The specific technical scheme is as follows:
the utility model provides a collection system that pig molecule genetic marker used, includes sampling station and blade holding tank, the blade holding tank is located the up end of sampling station, sampling station's top is provided with the second sleeve, but be provided with longitudinal movement's cutting mechanism in the second sleeve, cutting mechanism includes second longitudinal movement subassembly and second slider, the second slider passes through the longitudinal movement is realized to the second longitudinal movement subassembly, the below of second slider is provided with the connecting block, the lower extreme of connecting block is provided with cutting assembly, cutting assembly includes annular blade, just annular blade with the coaxial heart setting of blade holding tank.
Preferably, a first sleeve used for clamping the pig ear body is further arranged above the sampling table, the second sleeve is located in the first sleeve, and one side lower end of the first sleeve is connected with the sampling table through a first longitudinal moving assembly.
Preferably, the first longitudinal moving component comprises a sliding cavity, the sliding cavity is located inside one side surface of the first sleeve, the sliding cavity is arranged along the height direction of the first sleeve, a first sliding block capable of sliding longitudinally is arranged in the sliding cavity, the lower end of the first sliding block is vertically and downwards provided with a connecting rod, the other end of the connecting rod penetrates through the lower end surface of the first sleeve and is connected with the upper end surface of the sampling table, and a first spring is arranged between the upper end of the first sliding block and the sliding cavity.
Preferably, at least two first longitudinal moving assemblies are provided, a plurality of first longitudinal moving assemblies are all located on the same side face of the first sleeve, and the first longitudinal moving assemblies are uniformly distributed at equal intervals.
Preferably, the outer side of the second sleeve is connected with the inner wall of the first sleeve through a plurality of second connecting rods, and the second sleeve and the cutting mechanism longitudinally move along with the first sleeve.
Preferably, the second longitudinal movement assembly comprises a pressing cover, a blind hole with a downward opening is formed in the lower end of the pressing cover, the upper end of the second sliding block is connected with the top of the blind hole, a first annular groove is further formed in the top of the blind hole, a second annular groove is formed in the upper end face of the first sleeve, the first annular groove and the second annular groove are identical in size and are coaxially arranged, the second annular groove is located right below the first annular groove, a second spring is adapted between the first annular groove and the second annular groove, the upper end of the second spring is connected with the first annular groove, and the lower end of the second spring is connected with the second annular groove.
Preferably, a first annular limiting block is further arranged at the lower end of the inner wall of the blind hole, a second annular limiting block is further arranged at the upper end of the side face of the first sleeve, and the first annular limiting block is located below the second annular limiting block.
Preferably, the upper end of the annular blade is provided with an annular blade holder, an inner ring of the annular blade holder is provided with an inner thread, the side surface of the lower end of the connecting block is provided with an outer thread which is connected with the inner thread in an adaptive manner, the lower end of the annular blade is provided with a blade, and two sides of the lower end of the blade, which are mutually symmetrical, are downwards arranged in a protruding manner. The same time period can reduce the cutting area when cutting the pig ear body, and is comparatively laborsaving, convenient cutting.
Preferably, the cutting assembly further comprises an annular sponge body, the annular sponge body is sleeved on the outer side face of the connecting block, the annular sponge body and the annular blade are coaxially arranged, the outer diameter of the annular sponge body is larger than that of the annular blade, a plurality of annular protrusions are uniformly arranged on the outer side face of the annular sponge body along the length of the annular sponge body, a plurality of annular clamping grooves which are connected with the annular protrusions in an adapting mode are formed in the inner ring of the annular sponge body, the annular sponge body is a slow rebound sponge, the annular sponge body is made of polyurethane high-molecular polymer with an open unit structure, and the lower middle portion of the annular sponge body is immersed with povidone iodine sterilizing liquid, and the components and the weight percentage content of the iodine sterilizing liquid are as follows: povidone iodine 21% -30%; 2% -3% of citric acid; nonoxynol-10.1% -1.5%; 0.11% -0.2% of sodium hydroxide; 65.3% -75.79% of water; the middle upper part of the annular sponge body is immersed with gelatin compound blood stopping liquid.
Preferably, the inside of annular blade is coaxial to be provided with annular push pedal, the upper end of annular push pedal is connected with the threaded rod through the coaxial heart of bearing, the upper end of threaded rod runs through in proper order connecting block, second slider, pressing plate are located the top of pressing plate, just the upper end of threaded rod is provided with the knob, be provided with in the connecting block with threaded hole that threaded rod adaptation is connected, just the outside diameter of annular push pedal is less than the inside diameter of annular blade holder does not hinder annular blade's the taking off.
Preferably, in order to further improve the stability of the annular blade during use, that is, the lowest end of the blade protruding portion is not in contact with the pig ear body before the first sleeve is clamped, the stiffness coefficient of the first spring is k1, the distance between the lowest end of the first sleeve and the upper end of the sampling table is x1, the first object weight is g1, the stiffness coefficient of the second spring is k2, the distance between the lowest ends of the blade protruding portion and the lowest ends of the first sleeve is x2, and the second object weight is g2 and the pressing force F should satisfy the following relationship:
in the above formula, F is a unit, N; k1, k2 units, N/m; x1, x2 units, m; alpha is a relation coefficient, and the value range is 1.0-1.22; g1 is the total weight of the first sleeve, the second sleeve and the cutting mechanism, and the unit is N; g2 is the total weight of the cutting mechanism in N.
Preferably, the invention also discloses a collection method of the collection device for pig molecular genetic markers, which comprises the following specific steps:
s1: taking down the annular blade from the connecting block and taking down the annular sponge body;
s2: the middle lower part of the annular sponge body is immersed with polyvidone iodine sterilizing disinfectant, and the middle lower part of the annular sponge body is immersed with gelatin compound blood stopping liquid;
s3: sleeving the annular sponge body on the connecting block, and connecting the annular blade with the connecting block;
s4: placing the pig ear body on a sampling table, and pressing down the pressing cover by hand;
s5: the lower end of the first sleeve is contacted with the pig ear body through the pressing in the first stage, so that clamping is realized;
s6: the annular blade starts to cut through the pressing of the second stage and carries the cut sample into the blade accommodating groove;
s7: through the pressing of the third stage, the polyvidone iodine sterilizing and disinfecting liquid and the gelatin compound hemostatic liquid which are immersed on the annular sponge body are sterilized and hemostatic with the pig ear cutting wound in sequence;
s8: and loosening the pressing cover, taking out the sample from the annular blade through the knob, and storing in time.
Compared with the prior art, the invention has the following beneficial effects:
(1) According to the collecting device for pig molecular genetic markers, the detachable annular blade and the annular sponge body are arranged on the connecting block, the middle lower part of the annular sponge body is immersed with the polyvidone iodine sterilizing disinfectant, and the middle upper part of the annular sponge body is immersed with the gelatin compound hemostatic liquid, so that the polyvidone iodine sterilizing disinfectant and the gelatin compound hemostatic liquid immersed on the annular sponge body can sterilize, disinfect and stanch and feed the pig ear cutting wound after the annular blade is sampled.
(2) According to the acquisition device for the pig molecular genetic marker, the first sleeve capable of moving longitudinally is arranged, the lower end of the first sleeve can be contacted with the pig ear body firstly through pressing in the first stage, so that the clamping function is realized, and the stability during cutting can be further improved.
(3) According to the collecting device for pig molecular genetic markers, the two sides of the lower end of the cutting edge, which are symmetrical with each other, are downwards convexly arranged, so that the cutting contact area when the pig ear body is cut can be reduced in the same time period during cutting, the labor is saved, and the cutting is convenient.
(4) According to the acquisition device for the pig molecular genetic marker, the stability of the annular blade in use can be further improved through limitation of the stiffness coefficient k1 of the first spring, the distance x1 between the lowest end of the first sleeve and the upper end of the sampling table, the first weight g1, the stiffness coefficient k2 of the second spring, the distance x2 between the lowest ends of the first sleeve and the second weight g2 of the blade protruding portion and the pressing force F, namely the lowest ends of the blade protruding portions are not contacted with a pig ear body before clamping of the first sleeve is achieved.
Drawings
Fig. 1 is a schematic structural view of the present invention.
Fig. 2 is an enlarged schematic view of a portion of fig. 1 of the present invention.
FIG. 3 is a schematic top view of a sampling station structure according to the present invention.
Fig. 4 is a schematic view of the first stage pressing structure of the present invention.
Fig. 5 is a schematic view of the structure of the present invention at the time of the second stage pressing.
In the figure: 1. a sampling table; 2. a blade receiving slot; 3. a first sleeve; 41. a sliding cavity; 42. a first slider; 43. a connecting rod; 44. a first spring; 5. a second sleeve; 61. a second slider; 62. a connecting block; 631. an annular tool apron; 632. an annular blade; 633. an annular sponge body; 71. pressing the cover; 72. a first annular groove; 73. a second annular groove; 74. a second spring; 75. the first annular limiting block; 76. the second annular limiting block; 8. an annular protrusion; 9. a threaded rod; 10. an annular push plate; 11. a knob; 12. round bench; 13. a pig ear body; 14. a bearing; 15. an anti-slip press pad; 16. and a second connecting rod.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
As shown in fig. 1-3, a collection device for pig molecular genetic marker comprises a sampling table 1 and a blade holding groove 2, wherein the blade holding groove 2 is positioned on the upper end surface of the sampling table 1, a second sleeve 5 is arranged above the sampling table 1, a longitudinally movable cutting mechanism is arranged in the second sleeve 5, the cutting mechanism comprises a second longitudinally movable component and a second sliding block 61, the second sliding block 61 realizes longitudinal movement through the second longitudinally movable component, a connecting block 62 is arranged below the second sliding block 61, a cutting component is arranged at the lower end of the connecting block 62, the cutting component comprises an annular blade 632, and the annular blade 632 and the blade holding groove 2 are coaxially arranged.
The top of sampling platform 1 still is provided with and is used for centre gripping pig ear body 13's first sleeve 3, second sleeve 5 is located in the first sleeve 3, just one side lower extreme of first sleeve 3 pass through first longitudinal movement subassembly with sampling platform 1 is connected. The lower end of the first sleeve 3 is provided with an anti-slip pressing pad 15.
The first longitudinal moving assembly comprises a sliding cavity 41, the sliding cavity 41 is located inside one side face of the first sleeve 3, the sliding cavity 41 is arranged along the height direction of the first sleeve 3, a first sliding block 42 capable of sliding longitudinally is arranged in the sliding cavity 41, the lower end of the first sliding block 42 is vertically and downwards provided with a connecting rod 43, the other end of the connecting rod 43 penetrates through the lower end face of the first sleeve 3 and is connected with the upper end face of the sampling table 1, and a first spring 44 is arranged between the upper end of the first sliding block 42 and the sliding cavity 41.
The first longitudinal moving assemblies are at least two, a plurality of the first longitudinal moving assemblies are all located on the same side face of the first sleeve 3, and the first longitudinal moving assemblies are uniformly distributed at equal intervals.
The outer side of the second sleeve 5 is connected with the inner wall of the first sleeve 3 through a plurality of second connecting rods 16, and the second sleeve 5 and the cutting mechanism longitudinally move along with the first sleeve 3.
The second longitudinal moving assembly comprises a pressing cover 71, a blind hole with a downward opening is formed in the lower end of the pressing cover 71, the upper end of the second sliding block 61 is connected with the top of the blind hole, a first annular groove 72 is further formed in the top of the blind hole, a second annular groove 73 is formed in the upper end face of the first sleeve 3, the first annular groove 72 and the second annular groove 73 are identical in size and are coaxially arranged, the second annular groove 73 is located right below the first annular groove 72, a second spring 74 is adapted between the first annular groove 72 and the second annular groove 73, the upper end of the second spring 74 is connected with the first annular groove 72, and the lower end of the second spring 74 is connected with the second annular groove 73.
The lower end of the inner wall of the blind hole is further provided with a first annular limiting block 75, the upper end of the side face of the first sleeve 3 is further provided with a second annular limiting block 75, and the first annular limiting block 75 is located below the second annular limiting block 75.
The upper end of annular blade 632 is provided with annular blade holder 631, the inner circle of annular blade holder 631 is provided with the internal thread, the lower extreme side of connecting block 62 be provided with the external screw thread of internal thread adaptation connection, the lower extreme of annular blade 632 is provided with the cutting edge, just the lower extreme of cutting edge is symmetrical both sides all bulge downwards setting each other. When cutting, the same time quantum can reduce the cutting area when cutting the pig ear body, and is comparatively laborsaving, convenient cutting. The principle is the same as that of the injection needle.
The cutting assembly further comprises an annular sponge body 633, the annular sponge body 633 is sleeved on the outer side surface of the connecting block 62, the annular sponge body 633 and the annular blade 632 are coaxially arranged, the outer diameter of the annular sponge body 633 is larger than that of the annular blade 632, a plurality of annular protrusions 8 are uniformly arranged on the outer side surface of the annular sponge body 633 along the length of the annular sponge body 633, a plurality of annular clamping grooves which are connected with the annular protrusions 8 in an adapting mode are formed in the inner ring of the annular sponge body 633, the annular sponge body 633 is a slow rebound sponge, the annular sponge body 633 is made of polyurethane high-molecular polymer with an open unit structure, and the lower middle portion of the annular sponge body 633 is immersed with a povidone iodine sterilizing liquid, and the components and weight percentage of the iodine sterilizing liquid are as follows: povidone iodine 21% -30%; 2% -3% of citric acid; nonoxynol-101.1% -1.5%; 0.11% -0.2% of sodium hydroxide; 65.3% -75.79% of water; the middle upper part of the annular sponge 633 is immersed with gelatin compound blood stopping liquid.
The detachable annular blade and the annular sponge body are arranged on the connecting block, the middle lower part of the annular sponge body is immersed with the povidone iodine sterilizing disinfectant, and the middle upper part of the annular sponge body is immersed with the gelatin compound hemostatic liquid, so that the annular blade can be ensured to be used for carrying out good sterilization, disinfection and hemostatic and medicine feeding on the cut wound of the pig ear after the annular blade is used for sampling, and the povidone iodine sterilizing disinfectant and the gelatin compound hemostatic liquid immersed on the annular sponge body can be carried out successively.
The inboard of annular blade 632 is provided with annular push pedal 10 coaxially, the upper end of annular push pedal 10 is connected with threaded rod 9 through the coaxial heart of bearing 14, the upper end of threaded rod 9 runs through in proper order connecting block 62, second slider 61, push plate 71 are located the top of push plate, just the upper end of threaded rod 9 is provided with knob 11, be provided with in the connecting block 62 with threaded hole that threaded rod 9 adaptation is connected, just the outside diameter of annular push pedal 10 is less than the inside diameter of annular blade holder 631 does not hinder annular blade's the dismantlement. The threaded rod 9 can be rotated through the knob 11, the annular push plate 10 moves downwards, samples can be ejected out of the annular blade, and collection is convenient.
The blade accommodating groove 2 is internally and coaxially provided with a round table 12, the height of the round table 12 is smaller than the depth of the blade accommodating groove 2, and the maximum diameter of the round table 12 is smaller than the inner diameter of the annular blade. Can prevent the sample from falling into the bottom of the blade holding groove 2, and is inconvenient to take out.
Example 2
On the basis of embodiment 1, in order to further improve the stability of the annular blade during use, namely, the lowest end of the blade protruding portion is not contacted with the pig ear body before the first sleeve is clamped, the stiffness coefficient of the first spring is k1, the distance between the lowest end of the first sleeve and the upper end of the sampling table is x1, the first object weight is g1, the stiffness coefficient of the second spring is k2, the distance between the lowest ends of the first sleeve and the lowest ends of the blade protruding portion is x2, the second object weight is g2, and the following relation should be satisfied:
in the above formula, F is a unit, N; k1, k2 units, N/m; x1, x2 units, m; alpha is a relation coefficient, and the value range is 1-1.22; g1 is the total weight of the first sleeve, the second sleeve and the cutting mechanism, and the unit is N; g2 is the total weight of the cutting mechanism in N.
Example 3
As shown in fig. 1-5, on the basis of embodiment 1, the invention also discloses a method for using the collection device for pig molecular genetic markers, which comprises the following specific steps:
s1: taking down the annular blade from the connecting block and taking down the annular sponge body;
s2: the middle lower part of the annular sponge body is immersed with polyvidone iodine sterilizing disinfectant, and the middle lower part of the annular sponge body is immersed with gelatin compound blood stopping liquid;
s3: sleeving the annular sponge body on the connecting block, and connecting the annular blade with the connecting block;
s4: placing the pig ear body on a sampling table, and pressing down the pressing cover by hand;
s5: the lower end of the first sleeve is contacted with the pig ear body through the pressing in the first stage, so that clamping is realized;
s6: the annular blade starts to cut through the pressing of the second stage and carries the cut sample into the blade accommodating groove;
s7: through the pressing of the third stage, the polyvidone iodine sterilizing and disinfecting liquid and the gelatin compound hemostatic liquid which are immersed on the annular sponge body are sterilized and hemostatic with the pig ear cutting wound in sequence;
s8: and loosening the pressing cover, taking out the sample from the annular blade through the knob, and storing in time.
The technical features of the above-described embodiments may be arbitrarily combined, and all possible combinations of the technical features of the above-described embodiments are not described for brevity of description, however, as long as there is no contradiction between the combinations of the technical features, they should be considered as the scope of the description.
The foregoing examples merely illustrate embodiments of the invention and are described in more detail and are not to be construed as limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention. Accordingly, the scope of protection of the present invention is to be determined by the appended claims.
Claims (4)
1. The utility model provides a collection system that pig molecule genetic marker used, its characterized in that includes sampling platform (1) and blade holding tank (2), blade holding tank (2) are located sampling platform (1)'s up end, sampling platform (1) top is provided with second sleeve (5), but be provided with longitudinal movement's cutting mechanism in second sleeve (5), cutting mechanism includes second longitudinal movement subassembly and second slider (61), second slider (61) are through the longitudinal movement of second longitudinal movement subassembly realization, the below of second slider (61) is provided with connecting block (62), the lower extreme of connecting block (62) is provided with cutting assembly, cutting assembly includes annular blade (632), just annular blade (632) with blade holding tank (2) coaxial setting;
a first sleeve (3) for clamping the pig ear body (13) is further arranged above the sampling table (1), the second sleeve (5) is positioned in the first sleeve (3), and the lower end of one side of the first sleeve (3) is connected with the sampling table (1) through a first longitudinal moving component;
the first longitudinal moving assembly comprises a sliding cavity (41), the sliding cavity (41) is positioned inside one side surface of the first sleeve (3), the sliding cavity (41) is arranged along the height direction of the first sleeve (3), a first sliding block (42) capable of longitudinally sliding is arranged in the sliding cavity (41), a connecting rod (43) is vertically and downwards arranged at the lower end of the first sliding block (42), the other end of the connecting rod (43) penetrates through the lower end surface of the first sleeve (3) and is connected with the upper end surface of the sampling table (1), and a first spring (44) is arranged between the upper end of the first sliding block (42) and the sliding cavity (41);
the second longitudinal moving assembly comprises a pressing cover (71), a blind hole with a downward opening is formed in the lower end of the pressing cover (71), the upper end of the second sliding block (61) is connected with the top of the blind hole, a first annular groove (72) is further formed in the top of the blind hole, a second annular groove (73) is formed in the upper end face of the first sleeve (3), the first annular groove (72) and the second annular groove (73) are identical in size and are coaxially arranged, the second annular groove (73) is located under the first annular groove (72), a second spring (74) is adapted between the first annular groove (72) and the second annular groove (73), the upper end of the second spring (74) is connected with the first annular groove (72), and the lower end of the second spring (74) is connected with the second annular groove (73).
The first spring (44) has a stiffness coefficient of k1, the distance between the lowest end of the first sleeve (3) and the upper end of the sampling table (1) is x1, the first object weight is g1, the second spring (74) has a stiffness coefficient of k2, the distance between the lowest end of the blade protruding part and the lowest end of the first sleeve (3) is x2, the second object weight is g2, and the pressing force F should satisfy the following relation:
in the above formula, F is a unit, N; k1, k2 units, N/m; x1, x2 units, m; alpha is a relation coefficient, and the value range is 1.0-1.22; g1 is the total weight of the first sleeve, the second sleeve and the cutting mechanism, and the unit is N; g2 is the total weight of the cutting mechanism in N.
2. The collection device for pig molecular genetic markers according to claim 1, wherein at least two first longitudinal moving assemblies are provided, a plurality of the first longitudinal moving assemblies are all located on the same side face of the first sleeve (3), and the first longitudinal moving assemblies are uniformly distributed at equal intervals.
3. The collecting device for pig molecular genetic markers according to claim 1, wherein the outer side of the second sleeve (5) is connected with the inner wall of the first sleeve (3) through a plurality of second connecting rods (16), and the second sleeve (5) and the cutting mechanism longitudinally move along with the first sleeve (3).
4. The collection device for pig molecular genetic markers according to claim 1, wherein a first annular limiting block (75) is further arranged at the lower end of the inner wall of the blind hole, a second annular limiting block (75) is further arranged at the upper end of the side face of the first sleeve (3), and the first annular limiting block (75) is located below the second annular limiting block (75).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111553697.1A CN114323738B (en) | 2021-12-17 | 2021-12-17 | Acquisition device for pig molecular genetic marker |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111553697.1A CN114323738B (en) | 2021-12-17 | 2021-12-17 | Acquisition device for pig molecular genetic marker |
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| CN114323738A CN114323738A (en) | 2022-04-12 |
| CN114323738B true CN114323738B (en) | 2023-11-07 |
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| CN202111553697.1A Active CN114323738B (en) | 2021-12-17 | 2021-12-17 | Acquisition device for pig molecular genetic marker |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN210494131U (en) * | 2019-06-20 | 2020-05-12 | 西藏新好科技有限公司 | Quick sampling device is used to pig house |
| CN112206015A (en) * | 2020-10-12 | 2021-01-12 | 张东伟 | Endoscope living body sampling device for respiration and critical illness |
| CN112294371A (en) * | 2020-11-23 | 2021-02-02 | 常培江 | Medical science is clinical with tumour biopsy sampling device |
| CN113331878A (en) * | 2021-06-02 | 2021-09-03 | 王宝菊 | Animal doctor's laboratory detects special animal tissue sampler |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2940010B1 (en) * | 2008-12-19 | 2011-11-11 | Affflex Europ | DEVICE FOR SAMPLING AN ANIMAL TISSUE SAMPLE |
| US20190321012A1 (en) * | 2017-01-03 | 2019-10-24 | Flower Medical Co., Ltd. | Disposable needle biopsy device and needle structure of cutting biopsy apparatus |
| WO2020015832A1 (en) * | 2018-07-19 | 2020-01-23 | Datamars Sa | System for sampling tissue |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN210494131U (en) * | 2019-06-20 | 2020-05-12 | 西藏新好科技有限公司 | Quick sampling device is used to pig house |
| CN112206015A (en) * | 2020-10-12 | 2021-01-12 | 张东伟 | Endoscope living body sampling device for respiration and critical illness |
| CN112294371A (en) * | 2020-11-23 | 2021-02-02 | 常培江 | Medical science is clinical with tumour biopsy sampling device |
| CN113331878A (en) * | 2021-06-02 | 2021-09-03 | 王宝菊 | Animal doctor's laboratory detects special animal tissue sampler |
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