CN114317643A - Preparation method of nicotinamide mononucleotide - Google Patents
Preparation method of nicotinamide mononucleotide Download PDFInfo
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- CN114317643A CN114317643A CN202210056475.7A CN202210056475A CN114317643A CN 114317643 A CN114317643 A CN 114317643A CN 202210056475 A CN202210056475 A CN 202210056475A CN 114317643 A CN114317643 A CN 114317643A
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- reaction
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- reaction tank
- nicotinamide mononucleotide
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- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- DAYLJWODMCOQEW-TURQNECASA-O NMN(+) Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP(O)(O)=O)O2)O)=C1 DAYLJWODMCOQEW-TURQNECASA-O 0.000 title abstract 5
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 21
- 239000007788 liquid Substances 0.000 claims abstract description 20
- 238000001914 filtration Methods 0.000 claims abstract description 9
- ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 claims abstract description 4
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 claims abstract description 4
- JLEBZPBDRKPWTD-TURQNECASA-O N-ribosylnicotinamide Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](CO)O2)O)=C1 JLEBZPBDRKPWTD-TURQNECASA-O 0.000 claims abstract description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 4
- 229940050906 magnesium chloride hexahydrate Drugs 0.000 claims abstract description 4
- DHRRIBDTHFBPNG-UHFFFAOYSA-L magnesium dichloride hexahydrate Chemical compound O.O.O.O.O.O.[Mg+2].[Cl-].[Cl-] DHRRIBDTHFBPNG-UHFFFAOYSA-L 0.000 claims abstract description 4
- 238000003756 stirring Methods 0.000 claims abstract description 4
- 229910021642 ultra pure water Inorganic materials 0.000 claims abstract description 4
- 239000012498 ultrapure water Substances 0.000 claims abstract description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 26
- DAYLJWODMCOQEW-TURQNECASA-N NMN zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP(O)([O-])=O)O2)O)=C1 DAYLJWODMCOQEW-TURQNECASA-N 0.000 claims description 17
- 238000001728 nano-filtration Methods 0.000 claims description 15
- 239000000706 filtrate Substances 0.000 claims description 6
- 239000012528 membrane Substances 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 6
- 238000000108 ultra-filtration Methods 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 5
- 102000004190 Enzymes Human genes 0.000 claims description 3
- 108090000790 Enzymes Proteins 0.000 claims description 3
- 239000013078 crystal Substances 0.000 claims description 3
- 238000002425 crystallisation Methods 0.000 claims description 3
- 230000008025 crystallization Effects 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 238000004806 packaging method and process Methods 0.000 claims description 3
- 238000005086 pumping Methods 0.000 claims description 3
- 239000008213 purified water Substances 0.000 claims description 3
- 230000035484 reaction time Effects 0.000 claims description 3
- 238000007670 refining Methods 0.000 claims description 3
- 239000011347 resin Substances 0.000 claims description 3
- 229920005989 resin Polymers 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 239000012295 chemical reaction liquid Substances 0.000 claims description 2
- 235000020956 nicotinamide riboside Nutrition 0.000 abstract description 3
- 239000011618 nicotinamide riboside Substances 0.000 abstract description 3
- 239000000047 product Substances 0.000 description 2
- 240000007124 Brassica oleracea Species 0.000 description 1
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 1
- 235000011299 Brassica oleracea var botrytis Nutrition 0.000 description 1
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 1
- 235000017647 Brassica oleracea var italica Nutrition 0.000 description 1
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 1
- 240000003259 Brassica oleracea var. botrytis Species 0.000 description 1
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 244000025272 Persea americana Species 0.000 description 1
- 235000008673 Persea americana Nutrition 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000020256 human milk Nutrition 0.000 description 1
- 210000004251 human milk Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004770 neurodegeneration Effects 0.000 description 1
- 208000015122 neurodegenerative disease Diseases 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a preparation method of nicotinamide mononucleotide, which comprises the following steps of S1: putting nicotinamide riboside, adenosine triphosphate and magnesium chloride hexahydrate into ultrapure water in a reaction tank, and stirring for reaction, wherein the reaction temperature is S2: adding sodium hydroxide into the reacted solution to adjust the pH, adjusting the reaction solution to a proper temperature, and performing S3: diatomite is added into a reaction tank, the reaction tank feed liquid is pumped into a plate frame for filtration, clear liquid is collected, the technical field of preparation of nicotinamide mononucleotide is involved, the simplification of the preparation process of nicotinamide mononucleotide is realized through the preparation process of S1-S11, the product quality is ensured, and the yield of nicotinamide mononucleotide products is greatly improved.
Description
Technical Field
The invention relates to the technical field of nicotinamide mononucleotide preparation, in particular to a preparation method of nicotinamide mononucleotide.
Background
Nicotinamide mononucleotide is a substance contained in the human body, and is present in breast milk and food, such as green soybean, broccoli, cucumber, cabbage, avocado, tomato, etc., but is present in a very small amount (0.25 to 1.88 mg per 100 g).
The research of nicotinamide mononucleotide for improving the aging index is supported by the journal of proprietary Wei Science, and the research of a plurality of authoritative journals such as Nature, Science and Cell proves the function of NMN in the aspect of neurodegenerative diseases.
The existing preparation method of nicotinamide mononucleotide is complex, the yield is low, and the preparation process is time-consuming and labor-consuming.
Disclosure of Invention
The present invention aims to provide a method for preparing nicotinamide mononucleotide, which solves the problems of the background art.
In order to achieve the purpose, the invention provides the following technical scheme:
a preparation method of nicotinamide mononucleotide comprises the following specific preparation steps:
s1: putting nicotinamide riboside, adenosine triphosphate and magnesium chloride hexahydrate into ultrapure water in a reaction tank, and stirring
Carrying out reaction;
s2: adding sodium hydroxide into the reacted solution to adjust the pH value, and then adjusting the reaction solution to a proper temperature;
s3: adding diatomite into the reaction tank, pumping the feed liquid of the reaction tank into a plate frame for filtering, and collecting clear liquid;
s4: filtering the clear filtrate with an ultrafiltration membrane of 3000-5000 daltons, and collecting the clear filtrate;
s5: adding enzyme into the obtained reaction liquid, reacting for a period of time, and then adding hydrochloric acid to adjust the pH value in the reaction tank;
s6: filtering the collected ultrafiltration clear liquid with a nanofiltration membrane, adding purified water into the nanofiltration concentrated liquid until the nanofiltration is concentrated
The liquid conductivity is less than or equal to 6000 us/cm;
s7: adding a proper amount of hydrochloric acid, and refining the nanofiltration concentrated solution in a resin column;
s8: the nanofiltration concentrated solution is pumped into a decoloring tank for decoloring;
s9: the decolored liquid is injected into a crystallizing tank for crystallization;
s10: extracting NMN crystal powder by using a centrifugal machine and a drying machine;
s11: and finally, crushing, detecting and packaging the powder.
As a further scheme of the invention: and adding sodium hydroxide into the S2 to adjust the pH value to 6.2 +/-0.4 and adjusting the temperature to 35 +/-2 ℃.
As a further scheme of the invention: the reaction time in S5 was 5 hours, 2000L of 2% hydrochloric acid was added, and the pH was adjusted to 3.2. + -. 0.4.
As a further scheme of the invention: to the S7 was added 2% hydrochloric acid with 1800 liters of hydrochloric acid.
Compared with the prior art, the invention has the beneficial effects that: the preparation process of S1-S11 realizes the introduction of the preparation process of nicotinamide mononucleotide, ensures the quality of products and greatly improves the yield of nicotinamide mononucleotide products.
Drawings
FIG. 1 is a schematic diagram of a process for preparing nicotinamide mononucleotide.
Detailed Description
The technical solution of the present patent will be described in further detail with reference to the following embodiments.
Referring to FIG. 1, a method for preparing nicotinamide mononucleotide includes the following steps:
s1: putting nicotinamide riboside, adenosine triphosphate and magnesium chloride hexahydrate into ultrapure water in a reaction tank, and stirring
Carrying out reaction;
s2: adding sodium hydroxide into the reaction solution to adjust pH, adjusting the reaction solution to a proper temperature, adding sodium hydroxide to adjust pH to 6.2 + -0.4, and adjusting the temperature to 35 + -2 deg.C
S3: adding diatomite into the reaction tank, pumping the feed liquid of the reaction tank into a plate frame for filtering, and collecting clear liquid;
s4: filtering the clear filtrate with an ultrafiltration membrane of 3000-5000 daltons, and collecting the clear filtrate;
s5: adding enzyme into the obtained reaction solution, reacting for a period of time, adding hydrochloric acid to adjust pH value in the reaction tank, wherein the reaction time is 5 hours, adding 2% hydrochloric acid of 2000L, and adjusting pH to 3.2 + -0.4
S6: filtering the collected ultrafiltration clear liquid with a nanofiltration membrane, adding purified water into the nanofiltration concentrated liquid until the nanofiltration is concentrated
The liquid conductivity is less than or equal to 6000 us/cm;
s7: adding a proper amount of hydrochloric acid, refining the nanofiltration concentrated solution in a resin column, and adding 2% hydrochloric acid with the addition of 1800 liters of hydrochloric acid;
s8: the nanofiltration concentrated solution is pumped into a decoloring tank for decoloring;
s9: the decolored liquid is injected into a crystallizing tank for crystallization;
s10: extracting NMN crystal powder by using a centrifugal machine and a drying machine;
s11: and finally, crushing, detecting and packaging the powder.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art through specific situations.
Although the preferred embodiments of the present patent have been described in detail, the present patent is not limited to the above embodiments, and various changes can be made without departing from the spirit of the present patent within the knowledge of those skilled in the art.
Claims (4)
1. A preparation method of nicotinamide mononucleotide is characterized by comprising the following specific preparation steps:
s1: putting nicotinamide ribose, adenosine triphosphate and magnesium chloride hexahydrate into ultrapure water in a reaction tank, and stirring for reaction;
s2: adding sodium hydroxide into the reacted solution to adjust the pH value, and then adjusting the reaction solution to a proper temperature;
s3: adding diatomite into the reaction tank, pumping the feed liquid of the reaction tank into a plate frame for filtering, and collecting clear liquid;
s4: filtering the clear filtrate with an ultrafiltration membrane of 3000-5000 daltons, and collecting the clear filtrate;
s5: adding enzyme into the obtained reaction liquid, reacting for a period of time, and then adding hydrochloric acid to adjust the pH value in the reaction tank;
s6: the collected ultrafiltration clear liquid passes through a nanofiltration membrane, and purified water is added into the nanofiltration concentrated liquid until the conductivity of the nanofiltration concentrated liquid is less than or equal to 6000 us/cm;
s7: adding a proper amount of hydrochloric acid, and refining the nanofiltration concentrated solution in a resin column;
s8: the nanofiltration concentrated solution is pumped into a decoloring tank for decoloring;
s9: the decolored liquid is injected into a crystallizing tank for crystallization;
s10: extracting NMN crystal powder by using a centrifugal machine and a drying machine;
s11: and finally, crushing, detecting and packaging the powder.
2. The method of claim 1, wherein said S2 is pH adjusted to 6.2 + -0.4 by adding NaOH, and temperature is adjusted to 35 + -2 ℃.
3. The method of claim 1, wherein said reaction time of S5 is 5 hours, said pH is adjusted to 3.2 + -0.4 with 2000L of 2% HCl.
4. The method of claim 1, wherein said S7 is supplemented with 1800L of 2% HCl.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210056475.7A CN114317643A (en) | 2022-01-18 | 2022-01-18 | Preparation method of nicotinamide mononucleotide |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210056475.7A CN114317643A (en) | 2022-01-18 | 2022-01-18 | Preparation method of nicotinamide mononucleotide |
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| Publication Number | Publication Date |
|---|---|
| CN114317643A true CN114317643A (en) | 2022-04-12 |
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| CN202210056475.7A Pending CN114317643A (en) | 2022-01-18 | 2022-01-18 | Preparation method of nicotinamide mononucleotide |
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20160333041A1 (en) * | 2015-03-16 | 2016-11-17 | Bontac Bio-Engineering (Shenzhen) Co., Ltd | Method for purifying beta-nicotinamide mononucleotide |
| WO2018120069A1 (en) * | 2016-12-29 | 2018-07-05 | 苏州汉酶生物技术有限公司 | METHOD FOR ENZYMATICALLY PREPARING β-NICOTINAMIDE MONONUCLEOTIDE |
| CN111635917A (en) * | 2020-06-11 | 2020-09-08 | 辽宁天华生物药业有限公司 | Preparation method of beta-nicotinamide ribodinucleotide |
| CN111705096A (en) * | 2020-06-29 | 2020-09-25 | 上海舒泽生物科技研究所 | Method for producing beta-nicotinamide mononucleotide by enzyme conversion method |
| CN112225759A (en) * | 2020-10-22 | 2021-01-15 | 闵令涛 | Preparation and purification method of beta-nicotinamide mononucleotide solution |
| CN112608910A (en) * | 2020-12-15 | 2021-04-06 | 深圳希吉亚生物技术有限公司 | Nicotinamide ribokinase and application thereof |
| CN113121629A (en) * | 2021-03-25 | 2021-07-16 | 沁浩膜技术(厦门)有限公司 | Method for extracting nicotinamide mononucleotide from fermentation liquor |
| CN115433750A (en) * | 2022-09-13 | 2022-12-06 | 安徽瑞邦生物科技有限公司 | Preparation method of nicotinamide mononucleotide |
-
2022
- 2022-01-18 CN CN202210056475.7A patent/CN114317643A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20160333041A1 (en) * | 2015-03-16 | 2016-11-17 | Bontac Bio-Engineering (Shenzhen) Co., Ltd | Method for purifying beta-nicotinamide mononucleotide |
| WO2018120069A1 (en) * | 2016-12-29 | 2018-07-05 | 苏州汉酶生物技术有限公司 | METHOD FOR ENZYMATICALLY PREPARING β-NICOTINAMIDE MONONUCLEOTIDE |
| CN111635917A (en) * | 2020-06-11 | 2020-09-08 | 辽宁天华生物药业有限公司 | Preparation method of beta-nicotinamide ribodinucleotide |
| CN111705096A (en) * | 2020-06-29 | 2020-09-25 | 上海舒泽生物科技研究所 | Method for producing beta-nicotinamide mononucleotide by enzyme conversion method |
| CN112225759A (en) * | 2020-10-22 | 2021-01-15 | 闵令涛 | Preparation and purification method of beta-nicotinamide mononucleotide solution |
| CN112608910A (en) * | 2020-12-15 | 2021-04-06 | 深圳希吉亚生物技术有限公司 | Nicotinamide ribokinase and application thereof |
| CN113121629A (en) * | 2021-03-25 | 2021-07-16 | 沁浩膜技术(厦门)有限公司 | Method for extracting nicotinamide mononucleotide from fermentation liquor |
| CN115433750A (en) * | 2022-09-13 | 2022-12-06 | 安徽瑞邦生物科技有限公司 | Preparation method of nicotinamide mononucleotide |
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Application publication date: 20220412 |
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