CN114276950A - Wide-pH-adaptability salt-tolerant phosphate solubilizing bacterium and application thereof - Google Patents
Wide-pH-adaptability salt-tolerant phosphate solubilizing bacterium and application thereof Download PDFInfo
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Abstract
The invention discloses a wide-range pH salt-tolerant phosphate solubilizing bacterium and application thereof, wherein the wide-range pH salt-tolerant phosphate solubilizing bacterium is pseudomonas PSBDYJP20210001 which can tolerate the environmental conditions of pH 2-10 and NaCl concentration 1-5% and has a higher phosphate solubilizing function, and the pseudomonas PSBDYJP20210001 is preserved in: china center for type culture Collection, the collection addresses are: wuhan, China, the preservation date is: 13/09/2021, accession number: CCTCC NO: M20211169. The seeds of the suaeda salsa are soaked in the culture solution of the pseudomonas PSBDYJP20210001, so that the germination rate of the seeds can be obviously improved; the culture solution of the pseudomonas PSBDYJP20210001 is used for irrigating the soil for planting the suaeda salsa, so that the plant height, the root length, the root diameter and the root tip number of the suaeda salsa can be obviously improved.
Description
Technical Field
The invention relates to a strain and application thereof, in particular to a salt-tolerant phosphate-solubilizing bacterium with wide pH value and application thereof, belonging to the technical field of microorganisms.
Background
Phosphorus is one of mineral elements necessary for plant growth and development, is an important material basis for plant growth, and the amount of phosphorus absorbed by plants is in positive correlation with biomass and yield thereof. Most of phosphorus in the soil exists in an inorganic phosphorus or organic phosphide state and cannot be absorbed and utilized by plants. Phosphate Solubilizing bacteria (Phosphate Solubilizing bacteria) are bacteria which can convert insoluble phosphorus into phosphorus which can be absorbed and utilized by plants so as to improve the phosphorus absorption of the plants and further promote the plant growth, and the screening of the Phosphate Solubilizing bacteria with the characteristic of high-efficiency Phosphate solubilization has become a hot research topic in the technical field of agricultural microorganisms in China.
The total area of the saline-alkali soil in China exceeds about 14.87 hundred million mu, and accounts for 4.88 percent of the total available soil area in China. Meanwhile, the extensive expansion of facility agriculture in coastal areas leads to excessive exploitation of underground water, further to the salinity exceeding of seawater, cultivated soil and irrigation water, and seriously restricts the growth of terrestrial vegetation. The phosphate solubilizing bacteria are used for dissolving and releasing phosphorus in soil, so that the growth of plants is promoted, and the phosphate solubilizing bacteria have positive ecological significance for improving the land utilization efficiency.
At present, the reported phosphate solubilizing bacteria have different phosphate solubilizing efficiencies, different phosphate solubilizing mechanisms and different application effects in saline-alkali soil no matter in different genera or different strains in the same genus. Therefore, screening the phosphate solubilizing bacteria which can tolerate acid-base and high-salt environments and exploring the optimal application amount of the phosphate solubilizing bacteria have important ecological significance for promoting plant growth and improving land utilization efficiency.
Disclosure of Invention
The invention aims to provide a phosphate solubilizing bacterium with wide pH adaptation and salt tolerance, which can resist acid and alkali and high-salt environment.
The second purpose of the invention is to provide the application of the salt-tolerant phosphate-solubilizing bacteria with wide pH range in the environment with the pH of 2-10 and the mass concentration of NaCl of 1-5%.
In order to achieve the first object, the invention adopts the following technical scheme:
the wide-adaptation pH salt-tolerant phosphate-solubilizing bacterium is Pseudomonas PSBDYJP20210001, the Latin name is Pseudomonas pseudomonassasp PSBDYJP20210001, the wide-adaptation pH salt-tolerant phosphate-solubilizing bacterium can tolerate the environmental conditions of pH 2-10 and NaCl concentration 1-5%, and has a higher phosphate-solubilizing function, and the Pseudomonas PSBDYJP20210001 is preserved in: china center for type culture Collection, the collection addresses are: wuhan, China, the preservation date is: 13/09/2021, accession number: CCTCC NO: M20211169.
In order to achieve the second objective, the invention adopts the following technical scheme:
1. the application of the salt-tolerant phosphate-solubilizing bacteria with wide pH range (pseudomonas PSBDYJP20210001) in promoting the germination of the suaeda salsa seeds is characterized in that the step of promoting the germination of the suaeda salsa seeds comprises the following steps:
(1) culturing pseudomonas PSBDYJP20210001 to logarithmic phase by LB enrichment medium, then inoculating to NBRIP liquid culture medium, and performing shake culture at 30 ℃ and 220r/min for 24h to obtain phosphate solubilizing bacteria liquid;
(2) sterilizing Suaeda salsa seeds;
(3) and (3) soaking the sterilized suaeda salsa seeds obtained in the step (2) by the phosphate solubilizing bacteria liquid obtained in the step (1) for 5 days.
2. The application of the phosphate-solubilizing bacteria with wide pH adaptation (Pseudomonas PSBDYJP20210001) in promoting the growth of the Suaeda salsa plant is characterized in that the step of promoting the growth of the Suaeda salsa plant comprises the following steps:
(1) culturing pseudomonas PSBDYJP20210001 to logarithmic phase by LB enrichment medium, then inoculating to NBRIP liquid culture medium, and performing shake culture at 30 ℃ and 220r/min for 24h to obtain phosphate solubilizing bacteria liquid;
(2) sterilizing Suaeda salsa seeds, and then putting the Suaeda salsa seeds into an incubator at 28 ℃ for culturing until the seeds germinate;
(3) irrigating the soil with water to enable the water content of the soil to reach 80% of the maximum water holding capacity, then irrigating the soil with the phosphate solubilizing bacterium liquid obtained in the step (1), and then planting the germinated suaeda salsa seeds obtained in the step (2) into the soil within 24 hours.
The invention has the advantages that:
(1) the pseudomonas PSBDYJP20210001 which is the salt-tolerant phosphate solubilizing bacterium with the wide pH value and is obtained by screening can tolerate the environmental conditions of pH value of 2-10 and NaCl concentration of 1-5%, and has a higher phosphate solubilizing function; the culture solution of the pseudomonas PSBDYJP20210001 is used for soaking the seeds of the suaeda salsa, so that the germination rate of the seeds of the suaeda salsa can be obviously improved; the culture solution of the pseudomonas PSBDYJP20210001 is used for irrigating the soil for planting the suaeda salsa, so that the plant height, the root length, the root diameter and the root tip number of the suaeda salsa can be obviously improved.
(2) The pseudomonas PSBDYJP20210001 which is the salt-tolerant phosphate-solubilizing bacteria with wide pH value and is obtained by screening promotes the absorption and utilization of plants by converting insoluble or insoluble phosphorus in soil into quick-acting phosphorus or soluble phosphorus to be stored in the soil, thereby promoting the growth of the plants and having positive ecological significance for improving the utilization efficiency of land.
Drawings
FIG. 1 is a phosphorus-solubilizing circle diagram of Pseudomonas PSBDYJP20210001 screened by the invention;
FIG. 2 is a phylogenetic tree of Pseudomonas PSBDYJP20210001 screened by the present invention;
FIG. 3 is a graph showing the phosphate solubilizing capacity of Pseudomonas PSBDYJP20210001 screened by the present invention in the range of pH 2 to 10;
FIG. 4 is a graph showing the phosphate solubilizing amount of the Pseudomonas PSBDYJP20210001 screened by the present invention in the range of NaCl mass concentration of 1 to 5%.
Detailed Description
The invention is described in detail below with reference to the figures and the embodiments.
First, culture medium
1. Monkina inorganic phosphorus solid culture medium
The formula of the Monkina inorganic phosphorus solid culture medium is as follows: glucose 10g, (NH)4)2SO4 0.5g，NaCl 10g，KCl 0.3g，MgSO4·7H2O 0.3g，FeSO4·7H 20 0.036g， MnS04·4H2O 0.03g，Ca3(P04)25g, agar 18g, distilled water 1000mL, Tris-HCl adjusted pH to 8.
2. LB enrichment medium
The formulation of LB enriched medium is as follows: 10g of tryptone, 5g of yeast powder, 10g of NaCl and 1000mL of distilled water.
3. NBRIP liquid medium
The formulation of NBRIP liquid medium is as follows: glucose 10g, Ca3(P04)2 5g，MgCl2 5g，MgSO40.25g，KCl 0.2g，(NH 4)2SO40.1g, 1000mL of distilled water, and the pH was adjusted to 7.0.
Secondly, the separation, purification and identification of the pseudomonas PSBDYJP20210001 which is a salt-tolerant phosphate-solubilizing bacterium with wide pH range
1. Separation and purification of Pseudomonas PSBDYJP20210001
The soil sample is taken from Shandong Ying yellow river mouth saline-alkali soil, 10g of the soil sample is placed in a 500mL conical flask filled with 90mL of distilled water, ten times of gradient dilution is carried out on the mixed solution, proper dilution is taken to be respectively coated on Monkina inorganic phosphorus solid culture medium (flat plate), the mixed solution is cultured for 4 days at 30 ℃ (figure 1), a bacterial colony with an obvious phosphorus-dissolving ring is selected and is subjected to streak purification, and the bacterial colony is stored for later use at 4 ℃ under the number PSBDYJP 20210001.
The strain PSBDYJP20210001 is shake-cultured in LB enrichment medium at 30 ℃ and 220r/min, and the strain is preserved when the bacterial liquid is turbid and wall-hung.
2. Identification of the Strain PSBDYJP20210001
16S rDNA amplification, sequencing and splicing are carried out on a strain PSBDYJP20210001 to obtain a gene sequence, BLAST analysis is carried out on the gene sequence and a microbial sequence in a Genbank database to find that the homology of the 16S rDNA sequence of the strain and pseudomonas is more than 99 percent, and the sequence of the strain is uploaded to the Genbank database to obtain an accession number of OL 673059.
Phylogenetic trees were constructed using the MEGA adjacency method, and the results are shown in FIG. 2. FIG. 2 shows that: the strain PSBDYJP20210001 is integrated with pseudomonas into one.
Thirdly, determination of the phosphate-solubilizing activity of Pseudomonas PSBDYJP20210001
Activating pseudomonas PSBDYJP20210001 by adopting LB enrichment medium, culturing at 30 ℃ to logarithmic growth phase, then inoculating into a conical flask filled with 100mL NBRIP liquid culture medium according to the inoculum size of 10%, and finally performing shaking culture on a shaking table at 30 ℃ for 24h at 220 r/min.
Values were recorded as 0mg, 0.2mg, 0.4mg, 0.6mg, 0.8mg and 1.0mg phosphorus standard series solutions simultaneously with the blank at a wavelength of 700 nm. And drawing a phosphorus standard curve by taking the measured absorbance as a vertical coordinate and the phosphorus concentration as a vertical coordinate.
In order to measure the phosphorus-dissolving capacity of the pseudomonas PSBDYJP20210001, the phosphorus-dissolving amount of the pseudomonas PSBDYJP20210001 is measured by adopting a molybdenum-antimony-scandium colorimetric method, calcium phosphate is the only phosphorus source of inorganic phosphorus, and the inorganic phosphorus-dissolving capacity of the pseudomonas PSBDYJP20210001 is measured.
The phosphate solubilizing ability of pseudomonas PSBDYJP20210001 is compared with that of other phosphate solubilizing bacteria in Table 1.
TABLE 1 comparison of phosphate solubilizing Capacity
| Bacterial strains | PSBDYJP20210001 | QDZN-13 | DC-3-P |
| Phosphorus amount (mg/L) | 182±7.5 | 175.83±1.2 | 158.24 |
As can be seen from Table 1: the phosphate solubilizing capability of the pseudomonas PSBDYJP20210001 is stronger than that of common phosphate solubilizing bacteria.
Fourthly, determination of acid and alkali resistance range of pseudomonas PSBDYJP20210001
In order to measure the acid and alkali resistance range of the pseudomonas PSBDYJP20210001, the pseudomonas PSBDYJP20210001 is cultured to a logarithmic growth phase by using an LB enrichment culture medium, then the pseudomonas PSBDYJP20210001 is inoculated into a conical flask filled with 100mL NBRIP liquid culture medium in an inoculation amount of 10%, the pH value is adjusted by using HCl and NaOH, then the conical flask is shake-cultured for 24 hours at 30 ℃ and 220r/min, finally the phosphorus dissolving amount of the pseudomonas PSBDYJP20210001 is measured by adopting a molybdenum-antimony-scandium colorimetric method, calcium phosphate is the only phosphorus source of inorganic phosphorus, and the inorganic phosphorus dissolving capacity of the pseudomonas PSBDYJP20210001 is measured.
The phosphorus-solubilizing amount of Pseudomonas PSBDYJP20210001 in the pH range of 2-10 is shown in FIG. 3.
The acid and alkali resistance range (pH) of the pseudomonas PSBDYJP20210001 is compared with that of other phosphate solubilizing bacteria shown in Table 2.
TABLE 2 acid and base resistance Range (pH) comparison
| Bacterial strains | PSBDYJP20210001 | QL1501 |
| Acid and alkali resistance range (pH) | 2～10 | 7～9 |
The phosphorus-dissolving amount of the pseudomonas PSBDYJP20210001 is compared with that of other phosphorus-dissolving bacteria in Table 3.
TABLE 3 comparison of phosphorus solubilizing amounts
As can be seen from fig. 3 and tables 2 and 3: the acid-base resistance range and the phosphate-solubilizing quantity of the pseudomonas PSBDYJP20210001 are both stronger than those of common phosphate-solubilizing bacteria.
Fifthly, the influence of the culture solution (phosphate-solubilizing bacteria solution) of pseudomonas PSBDYJP20210001 with different pH values on the germination rate of the suaeda salsa seeds
Culturing the pseudomonas PSBDYJP20210001 to a logarithmic phase by using an LB enrichment culture medium, then adjusting the pH value by adding HCl and NaOH, wherein the pH value is respectively 2, 3, 4, 5, 6, 7, 8, 9 and 10, and then performing shake culture at 30 ℃ and 220r/min for 24h to obtain pseudomonas PSBDYJP20210001 culture solutions (namely phosphate solubilizing bacteria solutions with different pH values) with different pH values for later use.
Seed disinfection: sterilizing Suaeda salsa seeds with 70% alcohol for 1min, sterilizing Suaeda salsa seeds with 1% sodium hypochlorite for 15min, and washing the Suaeda salsa seeds with sterile water for 5 times.
Taking 30 sterilized culture dishes, dividing the culture dishes into 10 groups, wherein each group comprises 3 culture dishes, and 50 sterilized suaeda salsa seeds are placed in each culture dish. Adding the obtained 9 parts of phosphate-solubilizing bacteria solution with different pH values (pH values are respectively 2, 3, 4, 5, 6, 7, 8, 9 and 10) into the first 9 groups of culture dishes respectively to ensure that the suaeda salsa seeds are not salted, adding a Monkina inorganic phosphorus liquid culture medium into the 10 th group of culture dishes (serving as a control) to ensure that the suaeda salsa seeds are not salted, placing all the culture dishes into a 28 ℃ culture box, and waiting for the suaeda salsa seeds to germinate. The germination rate of the soaked suaeda salsa seeds in each culture dish is counted by regular observation every day.
The experiment was repeated 3 times and the results averaged.
The influence of the phosphate solubilizing bacteria solutions with different pH values on the germination rate of suaeda salsa is shown in Table 4.
TABLE 4 influence of phosphate solubilizing bacteria solutions with different pH values on the germination rate of Suaeda salsa seeds
Note: the lower case letters in the same column indicate significant difference (P < 0.05).
As can be seen from table 4: when the pH value of the phosphate-solubilizing bacterial liquid is 5, 6 or 7, the germination rate of the suaeda salsa seeds is obviously higher than that of a control group; when the pH value of the phosphate-solubilizing bacterial liquid is 5, the germination rate of the suaeda salsa seeds is obviously higher than that of the other phosphate-solubilizing bacterial liquids with different pH values (p is less than 0.05).
Sixthly, the influence of the culture solution (phosphate-solubilizing bacterium solution) of pseudomonas PSBDYJP20210001 with different pH values on the character of the suaeda salsa plant
Test soil: is taken from saline-alkali soil of east Shandong Ying yellow river estuary.
Phosphate solubilizing bacteria: pseudomonas PSBDYJP 20210001.
Plant: suaeda salsa.
The soil to be tested is filled into plastic pots with the height of 11cm, the pot mouth diameter of 13cm and the pot bottom diameter of 9cm, the dry weight of each pot of soil is 1kg, and distilled water is used for supplementing the soil moisture, so that the soil moisture content reaches 80% of the maximum water holding capacity.
Culturing the pseudomonas PSBDYJP20210001 to a logarithmic phase by using an LB enrichment culture medium, adjusting the pH value to be 2, 3, 4, 5, 6, 7, 8, 9 and 10 by using HCl and NaOH, respectively, and then performing shake culture at 30 ℃ and 220r/min for 24h to obtain pseudomonas PSBDYJP20210001 culture solutions (namely phosphate solubilizing bacteria solutions with different pH values) with different pH values, and meanwhile, setting the LB enrichment culture medium without adding the pseudomonas PSBDYJP20210001 as a control group.
Seed disinfection: sterilizing Suaeda salsa seeds with 70% alcohol for 1min, sterilizing Suaeda salsa seeds with 1% sodium hypochlorite for 15min, and washing the Suaeda salsa seeds with sterile water for 5 times.
Taking the sterilized culture dish, putting the sterilized suaeda salsa seeds into the culture dish, and putting the culture dish in an incubator at 28 ℃ for culturing until the seeds germinate.
Respectively irrigating an LB enrichment medium and phosphate solubilizing bacteria liquid with different pH values into different plastic pots, wherein each pot is 100mL, planting the germinated suaeda salsa seeds into the plastic pots within 24h, sowing 50 seeds in each pot, and measuring and counting the plant height of plants after sowing for 20 days; and meanwhile, a root system scanner is adopted to analyze the root system of the plant, and the root length, the root system diameter and the root tip number are specifically related.
The experiment was repeated 3 times and the results averaged.
The effect of the phosphate solubilizing bacteria solutions with different pH values on the plant characteristics of suaeda salsa is shown in Table 5.
TABLE 5 influence of phosphate solubilizing bacteria solutions with different pH values on overground and underground growth of Suaeda salsa
Note: the lower case letters in the same column indicate significant difference (P < 0.05).
As can be seen from table 5: when the pH of the phosphate-solubilizing bacterial liquid is 4, 5, 6 and 7, the plant height, the root length, the root diameter and the root tip number of the suaeda salsa plant are all higher than those of the control group; when the pH of the phosphate-solubilizing bacterial liquid is 2-9, the plant height and the root length of the suaeda salsa plant are higher than those of the control group; when the pH of the phosphate-solubilizing bacterial liquid is 5, the plant height, the root length, the root diameter and the root tip number of the suaeda salsa plants are all obviously higher than those of the other phosphate-solubilizing bacterial liquids with different pH values (p is less than 0.05).
Seventhly, determination of salinity tolerance range of pseudomonas PSBDYJP20210001
In order to measure the salinity tolerance range of the pseudomonas PSBDYJP20210001d, the salt concentration range is adjusted by adding NaCl, the phosphorus dissolving amount of the pseudomonas PSBDYJP20210001 is measured by adopting a molybdenum-antimony-scandium colorimetric method, calcium phosphate is the only inorganic phosphorus source, and the inorganic phosphorus dissolving capacity of the pseudomonas PSBDYJP20210001 is measured.
The phosphorus-dissolving amount of pseudomonas PSBDYJP20210001 under different salt concentration conditions is shown in figure 4.
The comparison of the phosphorus-dissolving amount of the pseudomonas PSBDYJP20210001 and other phosphorus-dissolving bacteria under different salt concentration conditions is shown in Table 6.
TABLE 6 comparison of phosphorus solubilizing amounts
As can be seen from fig. 4 and table 6: the salinity tolerance range of the pseudomonas PSBDYJP20210001 is stronger than that of common phosphate solubilizing bacteria.
Eighthly, influence of pseudomonas PSBDYJP20210001 culture solution (phosphate-solubilizing bacterium solution) with different salt concentrations on germination rate of suaeda salsa seeds
Culturing pseudomonas PSBDYJP20210001 to a logarithmic growth phase by using an LB enrichment culture medium, then inoculating the pseudomonas PSBDYJP20210001 to a triangular flask filled with 100mL NBRIP liquid culture medium in an inoculation amount of 10%, adjusting the salt concentration range by adding NaCl, wherein the salt concentration is respectively 1%, 2%, 3%, 4% and 5%, and then performing shake culture at 30 ℃ and 220r/min for 24h to obtain pseudomonas PSBDYJP20210001 culture solutions (namely phosphate-solubilizing bacteria solutions with different salt concentrations) with different salt concentrations for later use.
Seed disinfection: the method comprises the steps of disinfecting suaeda salsa seeds for 1min by using 70% alcohol, disinfecting the suaeda salsa seeds for 15min by using 1% sodium hypochlorite, and finally washing the suaeda salsa seeds for 5 times by using sterile water.
Taking 30 sterilized culture dishes, dividing the culture dishes into 6 groups, wherein each group comprises 5 culture dishes, and 50 sterilized suaeda salsa seeds are put into each culture dish. Respectively adding the phosphate-solubilizing bacteria solutions with 5 salt concentrations (the salt concentrations are respectively 1%, 2%, 3%, 4% and 5%) obtained in the above steps into the first 5 groups of culture dishes to ensure that the suaeda glauca seeds have no salt, adding a Monkinna inorganic phosphorus liquid culture medium into the 6 th group of culture dishes (serving as a control) to ensure that the suaeda glauca seeds have no salt, placing all the culture dishes into a 28 ℃ culture box, and waiting for the seeds to germinate. The germination rate of the soaked suaeda salsa seeds in each culture dish is counted by regular observation every day.
The experiment was repeated 3 times and the results averaged.
The influence of the phosphate solubilizing bacteria solutions with different salt concentrations on the germination rate of the suaeda salsa seeds is shown in table 7.
TABLE 7 influence of phosphate solubilizing bacteria solutions with different salt concentrations on the germination rate of Suaeda salsa seeds
Note: the lower case letters in the same column indicate significant difference (P < 0.05).
As can be seen from Table 7: on the 2 nd day of seed soaking, the phosphate solubilizing bacteria liquid with 1% salt concentration already shows better germination accelerating effect than the phosphate solubilizing bacteria liquid with other salt concentrations; from the 3 rd day of soaking the seeds, the effect of accelerating germination of the phosphate solubilizing bacterial liquid with the salt concentration of 2% on the suaeda salsa seeds is better than that of a control group; on the 5 th day of seed soaking, the phosphorus-dissolving bacteria liquid of the soaked seeds still keeps high pregermination effect (p is less than 0.05).
Ninth, the influence of the pseudomonas PSBDYJP20210001 phosphate-solubilizing bacterial liquid with different salt concentrations on the plant characters of the suaeda salsa
Test soil: is taken from saline-alkali soil of east Shandong Ying yellow river estuary.
Phosphate solubilizing bacteria: pseudomonas PSBDYJP 20210001.
Plant: suaeda salsa.
The soil to be tested is filled into plastic pots with the height of 11cm, the pot mouth diameter of 13cm and the pot bottom diameter of 9cm, the dry weight of each pot of soil is 1kg, and distilled water is used for supplementing the soil moisture, so that the soil moisture content reaches 80% of the maximum water holding capacity.
Culturing the pseudomonas PSBDYJP20210001 to a logarithmic phase by using an LB enrichment medium, then adjusting the salt concentration range by using NaCl, wherein the salt concentration is respectively 1%, 2%, 3%, 4% and 5%, and then performing shake culture at 30 ℃ and 220r/min for 24h to obtain pseudomonas PSBDYJP20210001 culture solutions (namely phosphate-solubilizing bacteria solutions with different salt concentrations) with different salt concentrations, and meanwhile, setting the LB enrichment medium without adding the pseudomonas PSBDYJP20210001 as a control group.
Seed disinfection: sterilizing Suaeda salsa seeds with 70% alcohol for 1min, sterilizing Suaeda salsa seeds with 1% sodium hypochlorite for 15min, and washing the Suaeda salsa seeds with sterile water for 5 times.
Taking the sterilized culture dish, putting the sterilized suaeda salsa seeds into the culture dish, and putting the culture dish in an incubator at 28 ℃ for culturing until the seeds germinate.
Respectively irrigating an LB enrichment culture medium and phosphate solubilizing bacteria liquid with different salt concentrations into different plastic pots, wherein each pot is 100mL, planting the germinated suaeda salsa seeds into the plastic pots within 24h, sowing 50 seeds in each pot, and measuring and counting the plant height of plants after sowing for 20 days; and meanwhile, a root system scanner is adopted to analyze the root system of the plant, and the root length, the root system diameter and the root tip number are specifically related.
The experiment was repeated 3 times and the results averaged.
The effect of the phosphate solubilizing bacteria liquid with different salt concentrations on the plant characteristics of suaeda salsa is shown in table 8.
TABLE 8 influence of phosphate solubilizing bacteria solutions of different salt concentrations on the plant characteristics of Suaeda salsa
Note: the lower case letters in the same column indicate significant difference (P < 0.05).
As can be seen from Table 8, when the salt concentration of the phosphate-solubilizing bacterial liquid is 1%, 2% or 3%, the plant height, root length, root diameter and root tip number of the Suaeda salsa plants are all higher than those of the control group; when the salt concentration of the phosphate-solubilizing bacterial liquid is 1%, the plant height, the root length, the root diameter and the root tip number of the suaeda salsa plants are all obviously higher than those of the phosphate-solubilizing bacterial liquid with the rest salt concentration (p is less than 0.05).
In conclusion, the pseudomonas PSBDYJP20210001 which is a salt-tolerant phosphate-solubilizing bacterium with a wide pH range and is obtained by screening can tolerate the environmental conditions of pH 2-10 and NaCl concentration 1-5%, and has a high phosphate-solubilizing function; the culture solution of the pseudomonas PSBDYJP20210001 is used for soaking the seeds of the suaeda salsa, so that the germination rate of the seeds of the suaeda salsa can be obviously improved; the culture solution of the pseudomonas PSBDYJP20210001 is used for irrigating the soil for planting the suaeda salsa, so that the plant height, the root length, the root diameter and the root tip number of the suaeda salsa can be obviously improved.
The pseudomonas PSBDYJP20210001 which is the salt-tolerant phosphate-solubilizing bacteria with wide pH value and is obtained by screening promotes the absorption and utilization of plants by converting insoluble or insoluble phosphorus in soil into quick-acting phosphorus or soluble phosphorus to be stored in the soil, thereby promoting the growth of the plants and having positive ecological significance for improving the utilization efficiency of land.
It should be noted that the above-mentioned embodiments do not limit the present invention in any way, and all technical solutions obtained by using equivalent alternatives or equivalent variations fall within the protection scope of the present invention.
Claims (4)
1. The wide-adaptation pH salt-tolerant phosphate-solubilizing bacterium is Pseudomonas PSBDYJP20210001, the Latin name is Pseudomonas PSBDYJP20210001, the wide-adaptation pH salt-tolerant phosphate-solubilizing bacterium can tolerate the environmental conditions of pH 2-10 and NaCl concentration 1-5%, and has a higher phosphate-solubilizing function, and the Pseudomonas PSBDYJP20210001 is preserved in: china center for type culture Collection, the collection addresses are: wuhan, China, the preservation date is: 13/09/2021, accession number: CCTCC NO: M20211169.
2. The application of the wide-pH-range salt-tolerant phosphate solubilizing bacteria as claimed in claim 1, wherein the step of promoting the germination of the suaeda salsa seeds comprises the following steps:
(1) culturing pseudomonas PSBDYJP20210001 to logarithmic phase by LB enrichment medium, then inoculating to NBRIP liquid culture medium, and performing shake culture at 30 ℃ and 220r/min for 24h to obtain phosphate solubilizing bacteria liquid;
(2) sterilizing Suaeda salsa seeds;
(3) and (3) soaking the sterilized suaeda salsa seeds obtained in the step (2) by the phosphate solubilizing bacteria liquid obtained in the step (1) for 5 days.
3. The use of the salt-tolerant phosphate-solubilizing bacteria with a broad pH range as claimed in claim 1 for promoting the growth of Suaeda salsa plants, wherein the step of promoting the growth of Suaeda salsa plants comprises:
(1) culturing pseudomonas PSBDYJP20210001 to logarithmic phase by LB enrichment medium, then inoculating to NBRIP liquid culture medium, and performing shake culture at 30 ℃ and 220r/min for 24h to obtain phosphate solubilizing bacteria liquid;
(2) sterilizing Suaeda salsa seeds, and then putting the Suaeda salsa seeds into an incubator at 28 ℃ for culturing until the seeds germinate;
(3) irrigating the soil with water to enable the water content of the soil to reach 80% of the maximum water holding capacity, then irrigating the soil with the phosphate solubilizing bacterium liquid obtained in the step (1), and then planting the germinated suaeda salsa seeds obtained in the step (2) into the soil within 24 hours.
4. The use of claim 3, wherein in step (3), the amount of the phosphate-solubilizing bacterial liquid poured is 100 mL/kg.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116333924A (en) * | 2023-02-22 | 2023-06-27 | 鲁东大学 | Biological organic conditioner special for saline-alkali soil and preparation method and application thereof |
| CN116333924B (en) * | 2023-02-22 | 2024-03-22 | 鲁东大学 | Biological organic conditioner special for saline-alkali soil and preparation method and application thereof |
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