CN114260074A

CN114260074A - Frozen animal tissue homogenate equipment

Info

Publication number: CN114260074A
Application number: CN202111582903.1A
Authority: CN
Inventors: 牛宝泉
Original assignee: Individual
Current assignee: Shenzhen Huarui Model Organism Technology Co ltd
Priority date: 2021-12-22
Filing date: 2021-12-22
Publication date: 2022-04-01
Anticipated expiration: 2041-12-22
Also published as: CN114260074B

Abstract

The invention relates to the field of homogenizing equipment, in particular to frozen animal tissue homogenizing equipment which comprises a base, wherein a nitrogen refrigerator is fixedly connected in the base, a cold chamber is also arranged in the base, the nitrogen refrigerator is communicated with the cold chamber through a cold air channel, the base is provided with a detachable stirring tank on the cold chamber, the cold chamber is fixedly connected with a temperature controller for controlling temperature at one side of the stirring tank, a pressure seat fixedly connected to a top plate is arranged above the base, a pressure rod groove is formed in the pressure seat, a pressure rod electrically slides in the pressure rod groove, the lower end of the pressure rod is fixedly connected with a working plate, the working plate is provided with a fixedly connected motor protective shell, a fixedly connected motor is arranged in the motor protective shell, the output end of the motor is fixedly connected with a driving shaft, the frozen tissue can be efficiently crushed, and the frozen animal tissue can be homogenized in three modes of scraping, cutting and grinding, the homogenization degree is improved, and the bubbles generated by the homogenization are removed, so that the homogenization quality is improved.

Description

Frozen animal tissue homogenate equipment

Technical Field

The invention relates to the field of homogenizing equipment, in particular to a homogenizing device for frozen animal tissues.

Background

The homogenizer is a machine for breaking up animal and plant tissues and grinding the animal and plant tissues into uniform paste, and the homogenizer is widely used for homogenizing animal tissues, biological samples, food, medicines, cosmetics, agricultural products, solid, semisolid and water-insoluble samples and can homogenize 20ml to 250 ml. The method is particularly suitable for preparing a microorganism detection sample, so that the process of extracting bacteria from the sample becomes very simple and rapid, only the sample and diluent (or emulsifier) are added into a sterilized cup, then the cup is pressed onto a homogenizer, and the machine is started for timing, a part of animal tissues needing to be treated need to be subjected to freeze thawing for multiple times before being homogenized, and the treatment of the homogenate of the frozen animal tissues at present has the following three problems:

1. the efficiency of repeatedly freezing and thawing animal tissues is low, and the current homogenizing equipment cannot homogenize the frozen animal tissues;

2. the common homogenizer has single action in the homogenizing process, cannot carry out multi-stage crushing on the frozen tissue, and has lower homogenizing efficiency and quality;

3. bubbles generated in the homogenizing process are easy to remove, and the subsequent experiments are influenced.

Disclosure of Invention

The invention aims to solve the problems in the prior art, and provides frozen animal tissue homogenizing equipment which can efficiently crush frozen tissues and homogenize the frozen animal tissues in three modes of scraping, cutting and grinding, so that the homogenizing degree is improved, and air bubbles generated by homogenizing are removed.

In order to achieve the purpose, the invention adopts the following technical scheme: the utility model provides a frozen animal tissue homogenate equipment, includes the base, be equipped with fixed connection's nitrogen gas refrigerator in the base, the cold chamber has still been seted up in the base, put through air conditioning way between nitrogen gas refrigerator and the cold chamber, the base is in be equipped with the detachable agitator tank on the cold chamber, the cold chamber is in the temperature controller of agitator tank one side fixed connection controlled temperature, the base top is equipped with the pressure seat of fixed connection on the roof, the pressure bar groove has been seted up in the pressure seat, the pressure bar is in the pressure bar inslot electricity slides, pressure bar lower extreme fixed connection working plate, be equipped with fixed connection's motor protective housing on the working plate, be equipped with fixed connection's motor in the motor protective housing, motor output fixed connection driving shaft.

Preferably, the driving shaft is provided with rotatable limit blocks which are fixedly connected, the driving shaft is provided with a fixed frame which is rotatably connected between the rotatable limit blocks, the two sides of the fixed frame are connected with a slidable limiting block in an up-and-down sliding way, the stirring tank is provided with a device for limiting the rotation of the slidable limiting block, the lower end of the fixed frame is provided with a tooth panel, the driving shaft is fixedly connected with a first gear at the tooth panel section, the driving shaft is connected with a rotary exhaust seat in a sliding way below the first gear, the rotary exhaust seat is symmetrically provided with rotatably connected supporting rods, the upper ends of the supporting rods are fixedly connected with a second gear meshed with the first gear, the supporting rods are also meshed with the tooth surface plate, and the two supporting rods are fixedly connected below the second gear and symmetrically and obliquely arranged together to rotate to form an air suction fan blade capable of generating suction to bottom air.

Preferably, it is equipped with fixed connection to rotate exhaust seat top the epaxial fixed spring piece of driving, fixed spring piece with be equipped with first spring between the rotation exhaust seat, the scraper that the circular array of driving shaft lower extreme fixed connection a plurality of set up, the groove of scraping that is used for scraping ice that staggers the setting is seted up downwards to the scraper, branch lower extreme fixed connection is to quilt the rotation cutter that ice bits that the scraper was scraped stir.

Preferably, the lower end of the driving shaft is provided with a grinding cavity, a fixed rod fixedly connected is arranged in the grinding cavity, and the upper end of the fixed rod is provided with a crushing blade fixedly connected and capable of further grinding crushed ice scraps below the rotary exhaust seat.

Preferably, the edge of the bottom of the rotary exhaust seat is symmetrically provided with two bubble collecting ports for collecting bubbles, a curved bubble inlet channel taking the bubble collecting ports as inlets is formed in the rotary exhaust seat, the rear section of the bubble inlet channel is an extrusion channel which gradually reduces the bubbles to be crushed, the rear section of the extrusion channel is a fine separation channel, the separation channel is divided into a separation air channel with the upper end used for exhausting upwards and an extrusion channel with the lower end used for exhausting slurry, the tail end of the extrusion channel is communicated with the outer interface of the lower end of the rotary exhaust seat, and the tail end of the separation air channel is communicated with the outer interface of the upper end of the rotary exhaust seat.

Preferably, the rotary exhaust seat is symmetrically provided with bubble pressing grooves at the extrusion channel, the bubble pressing grooves are connected with bubble pressing blocks for extruding bubbles in a sliding manner, a plurality of groups of second springs are arranged between the bubble pressing blocks and the bubble pressing grooves, and the opposite surfaces of the two bubble pressing blocks are provided with fixedly connected bubble breaking needles which are arranged in a staggered manner and used for breaking bubbles;

has the advantages that: the invention scrapes the frozen surface through the scraper and cuts the frozen animal tissue;

scraping the frozen tissue, and simultaneously homogenizing the frozen animal tissue in two modes of cutting and grinding to improve the homogenization degree;

meanwhile, the arrangement of the rotary exhaust seat enables bubbles generated by homogenate to be concentrated by centrifugal force, and the bubbles are removed through the extrusion channel, so that the quality of homogenate is improved.

Drawings

FIG. 1 is a schematic external view of the present invention;

FIG. 2 is a schematic diagram of a structural implementation of the present invention;

FIG. 3 is an enlarged view of the point A in FIG. 2;

FIG. 4 is an enlarged view of the point B in FIG. 2;

FIG. 5 is a schematic view taken along the line C-C in FIG. 3;

FIG. 6 is a schematic view of the direction D-D in FIG. 4;

FIG. 7 is a schematic view taken along the line E-E in FIG. 6;

fig. 8 is an enlarged view of F in fig. 6.

In the figure, a base 10, a nitrogen refrigerator 11, a cold chamber 12, a cold air channel 13, a stirring tank 14, a temperature controller 15, a pressure seat 16, a pressure rod 17, a pressure rod groove 18, a working plate 19, a motor protective shell 20, a motor 21, a driving shaft 22, a limiting groove 23, a fixed frame 24, a slidable limiting block 25, a rotatable limiting block 26, a support rod 27, a second gear 28, a first gear 29, an air suction fan blade 30, a tooth surface plate 31, a fixed spring block 32, a first spring 33, a rotatable exhaust seat 34, a bubble collecting port 35, a bubble inlet channel 36, a rotatable cutter 37, a scraper 38, a scraping groove 39, a grinding cavity 40, a fixed rod 41, a crushing blade 42, a separation channel 43, a separation air channel 44, a separation liquid channel 45, a squeezing channel 46, a bubble pressing groove 47, a bubble pressing block 48, a second spring 49 and a bubble breaking needle 50.

Detailed Description

The following are specific embodiments of the present invention and are further described with reference to the drawings, but the present invention is not limited to these embodiments.

In the description of the present invention, it should be noted that the terms "inside", "below", and the like refer to orientations or positional relationships based on the orientations or positional relationships shown in the drawings or orientations or positional relationships that the products of the present invention conventionally place when used, and are used only for convenience in describing the present invention and simplifying the description, but do not indicate or imply that the devices or elements referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like are used merely to distinguish one description from another, and are not to be construed as indicating or implying relative importance.

With reference to fig. 1-8, a frozen animal tissue homogenate device, which comprises a base 10, a nitrogen refrigerator 11 fixedly connected with the base 10 is arranged in the base 10, a cold chamber 12 is also arranged in the base 10, the nitrogen refrigerator 11 and the cold chamber 12 are communicated through a cold air channel 13, the base 10 is provided with a detachable stirring tank 14 on the cold chamber 12, the cold chamber 12 is fixedly connected with a temperature controller 15 for controlling temperature on one side of the stirring tank 14, a pressure seat 16 fixedly connected with a top plate is arranged above the base 10, a pressure rod groove 18 is arranged in the pressure seat 16, a pressure rod 17 electrically slides in the pressure rod groove 18, the lower end of the pressure rod 17 is fixedly connected with a working plate 19, a motor protection shell 20 fixedly connected with the working plate 19 is arranged on the working plate 20, a motor 21 fixedly connected with the motor is arranged in the motor protection shell 20, and the output end of the motor 21 is fixedly connected with a driving shaft 22.

Furthermore, a rotatable limiting block 26 fixedly connected with the driving shaft 22 is arranged on the driving shaft 22, a fixing frame 24 rotatably connected between the rotatable limiting blocks 26 is arranged on the driving shaft 22, the two sides of the fixing frame 24 are vertically and slidably connected with the slidable limiting blocks 25, a 51 limiting the rotation of the slidable limiting blocks 25 is arranged on the stirring tank 14, a toothed panel 31 is arranged at the lower end of the fixing frame 24, the driving shaft 22 is fixedly connected with a first gear 29 at the section of the toothed panel 31, the driving shaft 22 is slidably connected with a rotatable exhaust seat 34 below the first gear 29, rotatably connected supporting rods 27 are symmetrically arranged on the rotatable exhaust seat 34, a second gear 28 meshed with the first gear 29 is fixedly connected at the upper end of each supporting rod 27, the supporting rods 27 are further meshed with the toothed panel 31, and two supporting rods 27 are fixedly connected with an air suction fan blade 30 which is symmetrically arranged below the second gear 28 and obliquely and can generate suction force on the bottom air when rotating together.

Further, be equipped with fixed spring piece 32 of fixed connection on driving shaft 22 above the rotation exhaust seat 34, be equipped with first spring 33 between fixed spring piece 32 and the rotation exhaust seat 34, the scraper 38 that the circular array of a plurality of driving shaft 22 lower extreme fixed connection set up, the scraping groove 39 that is used for scraping the ice that staggers the setting is seted up downwards to scraper 38, the rotation cutter 37 that stirs is carried out to the ice bits of being scraped by scraper 38 to branch 27 lower extreme fixed connection.

Furthermore, a grinding cavity 40 is formed at the lower end of the driving shaft 22, a fixed rod 41 fixedly connected with the grinding cavity 40 is arranged in the grinding cavity 40, and a crushing blade 42 fixedly connected with the upper end of the fixed rod 41 and capable of further grinding crushed ice chips below the rotary exhaust seat 34 is arranged at the upper end of the fixed rod 41.

Furthermore, the bottom edge of the rotary exhaust seat 34 is symmetrically provided with two bubble collecting ports 35 for collecting bubbles, a curved bubble inlet channel 36 taking the bubble collecting port 35 as an inlet is formed in the rotary exhaust seat 34, the rear section of the bubble inlet channel 36 is a gradually reduced extrusion channel 46 for crushing bubbles, the rear section of the extrusion channel 46 is a fine separation channel 43, the separation channel 43 is divided into a separation air channel 44 with the upper end used for exhausting upwards and an extrusion channel 46 with the lower end used for exhausting slurry, the tail end of the extrusion channel 46 is communicated with the outer periphery of the lower end of the rotary exhaust seat 34, and the tail end of the separation air channel 44 is communicated with the outer periphery of the upper end of the rotary exhaust seat 34.

Furthermore, the rotary exhaust seat 34 is symmetrically provided with bubble pressing grooves 47 at the extrusion passage 46, the bubble pressing grooves 47 are connected with bubble pressing blocks 48 for extruding bubbles in a sliding manner, a plurality of groups of second springs 49 are arranged between the bubble pressing blocks 48 and the bubble pressing grooves 47, and the opposite surfaces of the two bubble pressing blocks 48 are provided with fixedly connected bubble breaking needles 50 which are arranged in a staggered manner and used for breaking bubbles.

The working principle is as follows: in an initial state, the pressure rod 17 is contracted in the pressure rod groove 18, the driving shaft 22 does not enter the stirring tank 14, the nitrogen refrigerator 11 is started, and nitrogen enters the cold chamber 12 through the cold air channel 13 to be cooled;

the worker puts animal tissues into a stirring tank 14 for freezing, closes a nitrogen refrigerator 11 after freezing, controls the temperature in a refrigerating chamber 12 to be between 0 and 4 ℃ all the time through a temperature controller 15, adds homogenate buffer solution into the stirring tank 14, controls a pressure seat 16 to extend out of a pressure rod groove 18, drives equipment at the lower end to enter the stirring tank 14 until a scraper 38 touches the ice surface, turns on a motor 21, the motor 21 drives a fixedly connected driving shaft 22 to rotate, the driving shaft 22 drives a scraper 38 at the bottom to rotate, simultaneously a pressure rod 17 keeps downward pressure, so that the scraper 38 has enough pressure on the ice surface to scrape the ice surface, because of the arrangement of the scraping groove 39 on the scraper 38, more ice chips are scraped each time, meanwhile, the scraped ice chips are pushed to a rotating cutter 37, because the driving shaft 22 rotates to drive a first gear 29 fixedly connected to rotate, the first gear 29 drives the meshed second gear 28 to rotate, meanwhile, the slidable limiting block 25 connected with the fixed frame 24 in a sliding way is limited on the stirring tank 14 and cannot rotate, so that the fixed frame 24 cannot rotate, the gear panel 31 below the stirring tank 2 is meshed with the second gear 28, so that the second gear 28 rotates around the driving shaft 22, and the second gear 28 is fixedly connected with the supporting rod 27, so that the supporting rod 27 rotates around the driving shaft 22, and the rotating cutter 37 fixedly connected below also rotates to crush the flaky and strip-shaped substances scraped by the scraper 38; the rotating exhaust seat 34 connected to the driving shaft 22 in a sliding way is pushed to move upwards against the elastic force of the first spring 33 due to the increasing of the materials scraped by the scraper 38, the existence of the rotating exhaust seat 34 can reduce the air entering and reduce the generation of air bubbles until the frozen tissues are completely ground, the materials in the stirring tank 14 are easily in a state that frozen objects and liquid exist simultaneously due to the existence of buffer solution and low temperature control, the rotating exhaust seat 34 is pressed on the liquid level, the fixed rod 41 is fixedly connected in the driving shaft 22, the driving shaft 22 drives the crushing blade 42 to rotate when rotating, the crushing blade 42 sucks the tissues in the homogenate at the upper end into the grinding cavity 40, and the tissues are further refined through the combined action of the further crushing of the crushing blade 42 and the grinding cavity 40 of the fixed rod 41 and simultaneously come out from the bottom of the driving shaft 22 for continuous circulation;

due to the arrangement of the bubble collecting port 35 at the bottom of the rotary exhaust seat 34, bubbles generated during stirring are accumulated at the bubble collecting port 35 under the action of centrifugal force and buoyancy and enter the bubble inlet channel 36 along with the rotation of the rotary exhaust seat 34, the bubbles enter the bubble inlet channel 36 and then are reduced in size through the extrusion channel 46 due to the passage at the extrusion channel 46, larger bubbles are crushed, meanwhile, if stubborn bubbles and fine bubbles are generated, the bubbles can be punctured by the bubble puncturing needle 50, further gas passes through the separation air channel 44 to the position above the rotary exhaust seat 34, liquid forming the bubbles enters the position below the rotary exhaust seat 34 again through the separation liquid channel 45, the suction fan blades 30 fixedly connected to the support rod 27 can rotate around the driving shaft 22 to generate suction force on the air below, the air pressure below the air is reduced, and further the bubbles are discharged through the separation air channel 44, so that the bubbles in the homogenate are avoided.

The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by using the contents of the present specification and the accompanying drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.

Claims

1. The frozen animal tissue homogenizing device comprises a base (10) and is characterized in that a nitrogen refrigerator (11) fixedly connected with the base (10) is arranged in the base (10), a cold chamber (12) is further formed in the base (10), the nitrogen refrigerator (11) and the cold chamber (12) are communicated through a cold air channel (13), the base (10) is provided with a detachable stirring tank (14) on the cold chamber (12), the cold chamber (12) is fixedly connected with a temperature controller (15) for controlling temperature on one side of the stirring tank (14), a pressure seat (16) fixedly connected with a top plate is arranged above the base (10), a pressure rod groove (18) is formed in the pressure seat (16), a pressure rod (17) electrically slides in the pressure rod groove (18), the lower end of the pressure rod (17) is fixedly connected with a working plate (19), and a motor protective shell (20) fixedly connected with the working plate (19) is arranged on the working plate (19), the motor protection shell (20) is internally provided with a fixedly connected motor (21), and the output end of the motor (21) is fixedly connected with a driving shaft (22).

2. A frozen animal tissue homogenisation device according to claim 1, wherein: the improved rotary exhaust device is characterized in that a rotatable limiting block (26) fixedly connected is arranged on the driving shaft (22), a fixing frame (24) rotatably connected between the rotatable limiting block (26) is arranged on the driving shaft (22), the two sides of the fixing frame (24) are vertically and slidably connected with the slidable limiting block (25), the stirring tank (14) is provided with a limiting mechanism for limiting the rotation of the slidable limiting block (25), a toothed panel (31) is arranged at the lower end of the fixing frame (24), the driving shaft (22) is fixedly connected with a first gear (29) at the section of the toothed panel (31), a rotary exhaust seat (34) is slidably connected below the first gear (29) in the driving shaft (22), rotatably connected supporting rods (27) are symmetrically arranged on the rotary exhaust seat (34), and the upper end of each supporting rod (27) is fixedly connected with a second gear (28) meshed with the first gear (29), the supporting rods (27) are also meshed with the toothed panel (31), and the two supporting rods (27) are fixedly connected with air suction fan blades (30) which are symmetrically and obliquely arranged below the second gear (28) and can generate suction force to bottom air when rotating together.

3. A frozen animal tissue homogenisation device according to claim 2, wherein: rotate exhaust seat (34) top and be equipped with fixed connection fixed spring piece (32) on driving shaft (22), fixed spring piece (32) with be equipped with first spring (33) between rotation exhaust seat (34), scraper (38) that the circular array of driving shaft (22) lower extreme fixed connection set up, the setting of staggering is seted up downwards in scraper (38) and is used for scraping groove (39) of scraping ice, branch (27) lower extreme fixed connection is to quilt the rotation cutter (37) that the ice bits that scraper (38) scraped carry out the stirring.

4. A frozen animal tissue homogenisation device according to claim 3, wherein: the grinding chamber (40) is formed in the lower end of the driving shaft (22), a fixing rod (41) fixedly connected is arranged in the grinding chamber (40), and crushing blades (42) fixedly connected and capable of further grinding crushed ice scraps below the rotary exhaust seat (34) are arranged at the upper end of the fixing rod (41).

5. An apparatus for homogenizing frozen animal tissue according to claim 4, characterized in that: rotate exhaust seat (34) bottom edge symmetry and offer two collection bubble mouths (35) that are used for collecting the bubble, it uses to have seted up in rotation exhaust seat (34) collection bubble mouth (35) is the curved bubble way (36) of advancing of entry as the entry, it is for the crushing extrusion way (46) of bubble to advance bubble way (36) back end, extrusion way (46) back end is tiny lane (43), lane (43) divide into upper end and are used for the carminative separation air flue (44) of upwards and lower extreme with thick liquid exhaust extrusion way (46), extrusion way (46) end with rotate exhaust seat (34) lower extreme outside of the face switch-on, separation air flue (44) end with rotate exhaust seat (34) upper end outside of the face switch-on.

6. An apparatus for homogenizing frozen animal tissue according to claim 5, characterized in that: the rotary exhaust seat (34) is symmetrically provided with bubble pressing grooves (47) at the extrusion channel (46), the bubble pressing grooves (47) are connected with bubble pressing blocks (48) for extruding bubbles in a sliding mode, a plurality of groups of second springs (49) are arranged between the bubble pressing blocks (48) and the bubble pressing grooves (47), and two bubble breaking needles (50) which are fixedly connected and staggered and are used for breaking bubbles are arranged on opposite surfaces of the bubble pressing blocks (48).