CN114259276A - High-activity bone construction method based on bone marrow aspiration enrichment system - Google Patents
High-activity bone construction method based on bone marrow aspiration enrichment system Download PDFInfo
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Abstract
The invention belongs to the technical field of bone tissue engineering, and particularly relates to a high-activity bone construction method based on a bone marrow aspiration enrichment system, which comprises the following steps: placing the allogeneic bone decalcification support into a prepared peptide solution for soaking, and washing after soaking to obtain the allogeneic bone decalcification support modified by the self-assembly polypeptide; placing the prepared enrichment material in an inner tank body of a mixing and stirring tank of a bone marrow suction enrichment system, and enriching according to preset time; and discharging waste liquid in the mixing and stirring tank after the enrichment is finished to obtain the constructed high-activity bone repair material. The invention can solve the problems of inaccurate bone taking mode and easy loss of osteogenic materials in the prior art.
Description
Technical Field
The invention belongs to the technical field of bone tissue engineering, and particularly relates to a high-activity bone construction method based on a bone marrow aspiration enrichment system.
Background
At present, with the rapid development of the medical industry, the technology in various medical fields is also rapidly developed, and various novel medical technologies are developed, but some defects exist, wherein the technology for repairing bone injuries and bone defects is a difficult problem in orthopedics. The existing method for solving the bone injury and bone defect is a bone transplantation method, and the method for solving the bone deficiency of the implantation site in the bone transplantation method is to transplant autologous bones, allogeneic bones, xenogeneic bones and artificial bones; the autologous bone transplantation has good histocompatibility, osteoconductivity, osteoinductivity and no immunogenicity, so the autologous bone transplantation method becomes the 'gold standard' of the bone transplantation method and has wide clinical application. However, since the source of autogenous bone is limited, it is difficult to satisfy the requirement of mass bone transplantation, and pain and appearance of the bone-taking part may be caused, or serious complications such as blood vessel, nerve injury, deep infectious hematoma, ilium wing fracture and the like may be caused, therefore, selecting proper autogenous bone material-taking site and material-taking method effectively reduces the trauma of autogenous bone transplantation supply area, which is the key point of the current autogenous bone transplantation research.
The traditional bone taking mode is that bone taking instruments such as an osteotome and a bone scoop are matched with repeated physical knocking to separate and take bones, the bone taking is inaccurate and is easy to damage peripheral vascular nerves, and in recent years, with the rapid development of regenerative medicine, stem cell therapy provides a new way for solving the problem of the existing refractory large bone defect and the future development of orthopedics. At present, the conventional material taking mode for obtaining bone marrow mesenchymal stem cells (BMSC) is puncturing and extracting suprailiac spines, sternum and ribs, but due to the limitation of the bone taking method and quality, the bone taking mode is inaccurate, and meanwhile, stem cell culture is required after bone taking, so that the stem cell loss is easily caused.
Disclosure of Invention
The invention aims to provide a high-activity bone construction method based on a bone marrow aspiration enrichment system, and aims to solve the problems that a bone taking mode is not accurate and an osteogenic material is easy to lose in the prior art.
The invention provides a basic scheme I: the utility model provides a marrow suction enrichment system, includes reaming drill bit, drilling rod, collecting pipe and mixing agitator tank, reaming drill bit can dismantle with the drilling rod and be connected, reaming drill bit and drilling rod are hollow, reaming drill bit is equipped with the drainage hole that a plurality of is eccentric negative angle and sets up, the drilling rod rear end is equipped with actuating mechanism, actuating mechanism and drilling rod sliding connection, collecting pipe parcel drilling rod, the collecting pipe rear end is equipped with water inlet and delivery port, water inlet and drilling rod afterbody intercommunication, the compounding agitator tank includes the outer jar of body and the inner tank body, be equipped with negative pressure suction device between the outer jar of body and the inner tank body, contain enrichment material and compounding agitating unit in the inner tank body, the delivery port passes through pipeline and outer jar of body intercommunication, inner tank body bottom is equipped with the vortex device.
The principle and the advantages of the first basic scheme provided by the invention are as follows: in the prior art, bone taking instruments such as an osteotome and a bone scoop are matched with repeated physical knocking to separate and take bones, so that the bone taking is not accurate and peripheral vascular nerves are easily injured, and a large amount of autologous bone material is lost in the bone taking process.
Therefore, aiming at the problems in the prior art, the driving mechanism is connected with the drill rod in a sliding way, before the drill rod starts to work, the driving mechanism is not contacted with the drill rod, the normal saline is injected into the drill rod by the water inlet, and when the drill rod works, the drill rod is propped against the driving mechanism, the drill rod is controlled to further control the marrow expanding drill bit to obtain the required bone raw material mixed liquid by means of negative pressure and store the bone raw material mixed liquid into the collecting pipe, then the bone raw material mixed liquid flows into the outer tank body of the mixing and stirring tank through the water outlet of the collecting pipe, meanwhile, the bone raw material is pumped into the inner tank body through the negative pressure suction device, the enrichment material in the inner tank body absorbs the osteogenic material in the bone raw material mixed liquid, the mixing and stirring device stirs the bone raw material mixed liquid, so that the enrichment material moves up and down to fully enrich the osteogenic material, meanwhile, the turbulence device enables the bone raw material settled at the bottom of the inner tank body to flow, so that the enriched material can be enriched with more osteogenic materials. Therefore, the invention has the advantages that: (1) the bone extraction in the backbone marrow cavity can be carried out under the guide of the guide pin by using the hollow drill bit, the marrow expanding drill bit and the drill rod are driven by the driving mechanism, and negative pressure suction is generated by high-speed rotation in the process of obtaining bone raw materials, so that the problem of inaccurate bone extraction cannot be caused by a fixed-point drilling mode; (2) the mixed liquid that flows into the outer jar gets into the inner tank by the negative pressure suction, the autologous bone material in the mixed liquid can be absorbed to the enrichment material of the inner tank body, negative pressure suction device suction liquid simultaneously gets into through the rotation of the inner tank body, promote compounding agitating unit to make the enrichment material multidirectional activity mix under the condition of normal collection, abundant enrichment autologous bone material, and the vortex device can make the autologous bone material that the inner tank bottom portion depositd flow in the inner tank, make the enrichment material can enrich more autologous bone materials.
Further, the enrichment material is an allogeneic bone decalcification support, and the surface of the allogeneic bone decalcification support is modified by self-assembly polypeptide.
Has the advantages that: the allogenic bone-based high-efficiency enrichment material utilizes the mild self-assembly characteristic of self-assembly polypeptide to perform surface modification on the allogenic bone, thereby improving the enrichment capacity and the adhesion characteristic of the enrichment material.
Further, the mixing and stirring device comprises a stirring shaft and stirring blades, wherein the stirring blades are positioned on the stirring shaft, one end of the stirring shaft is connected with the turbulence device, and the other end of the stirring shaft is connected with the external driving device.
Has the advantages that: can realize the stirring effect through the stirring leaf, and the (mixing) shaft is connected with the vortex device for the (mixing) shaft drives the vortex device when rotating and rotates, and then saves power device.
Further, the flow disturbing device is a propeller blade.
Has the advantages that: the turbulence effect can be achieved by the propeller blades.
Furthermore, a double-spiral structure is arranged on the drill rod, and a protection device is clamped and connected with the collecting pipe at the double-spiral position of the drill rod.
Has the advantages that: the drill rod is arranged to be of a double-spiral structure, local negative pressure is formed through high-speed rotation, unidirectional leading-out of osteogenic materials is facilitated, meanwhile, a protection device connected with the collecting pipe in a clamped mode is arranged at the position of the double spiral, the collecting pipe can be prevented from being scratched by the double-spiral structure, and stability is guaranteed.
Further, still include the waste liquid collecting tank, jar body bottom is equipped with liquid outlet and filter equipment in the mixing and stirring jar, the waste liquid collecting tank passes through the pipe connection liquid outlet.
Has the advantages that: can filter out the waste liquid through filter equipment and waste liquid collecting tank on the one hand, medical waste can be collected to on the other hand waste liquid collecting tank, avoids causing medical pollution.
Further, the reaming bit is an interchangeable reaming bit, and the gauge of the reaming bit is 10mm-13 mm.
Has the advantages that: the reaming bit is arranged to be a replaceable bit and in different specification ranges, and can be suitable for reaming of different parts.
Furthermore, the driving mechanism is a speed regulating motor, and the drill rod is positioned on a rotating shaft of the speed regulating motor.
Has the advantages that: the rotating speed of the reaming bit can be adjusted according to the actual situation by the speed regulating motor, and the applicability of the reaming system is improved.
The invention provides a second basic scheme: a high-activity bone construction method based on a bone marrow aspiration enrichment system comprises the following steps:
preparing an enriched material: placing the allogeneic bone decalcification support into a prepared peptide solution for soaking, and washing after soaking to obtain the allogeneic bone decalcification support modified by the self-assembly polypeptide;
an enrichment step: placing the prepared enrichment material in an inner tank body of a mixing and stirring tank of a bone marrow suction enrichment system, and enriching according to preset time;
constructing high-activity bones: and discharging waste liquid in the mixing and stirring tank after the enrichment is finished to obtain the constructed high-activity bone repair material.
The principle and the effect of the basic scheme II provided by the invention are as follows: in the invention, firstly, the allogeneic bone decalcified scaffold modified by self-assembly polypeptide is prepared, the allogeneic bone decalcified scaffold modified by self-assembly polypeptide is placed in a mixing and stirring tank of a bone marrow suction and enrichment system to enrich the needed bone forming material, the enrichment is completed after a period of time, and the waste liquid in the mixing and stirring tank is discharged to obtain the constructed high-activity bone repairing material. Therefore, the second basic scheme of the invention has the advantages that compared with the stem cell acquisition mode in the prior art, the method can be used for instantly enriching the stem cells in the process of collecting the stem cells, so that the problem of large loss of the stem cells is avoided, meanwhile, the obtained high-activity bone repair material is sufficient, and the frequent bone taking steps are reduced.
Further, the step of preparing the enriched material further comprises the following steps of:
constructing an osteoinductive membrane: filling the bone defect part with bone cement to form a bone induction membrane;
the step of constructing the high-activity bone further comprises the following steps:
repairing: taking out the bone cement, and implanting the constructed high-activity bone repair material into an induction membrane of a bone defect part.
Has the advantages that: the bone cement is filled in the bone defect part, the bone induction membrane with good osteogenic activity can be cultured, the constructed high-activity bone is implanted into the bone defect part, and the bone defect can be well repaired.
Drawings
FIG. 1 is a cross-sectional view of an enlarged portion of an embodiment of the present invention;
FIG. 2 is an overall view of an embodiment of the present invention;
FIG. 3 is a cross-sectional view of a reamer head according to an embodiment of the present invention;
FIG. 4 is a cross-sectional view of the tail of a collection tube according to an embodiment of the present invention;
FIG. 5 is an overall view of a mixing and agitating tank of an embodiment of the present invention;
FIG. 6 is a top plan overall view of a mixing and agitation tank of an embodiment of the present invention;
FIG. 7 is a block flow diagram of an embodiment of the present invention.
Detailed Description
The following is further detailed by way of specific embodiments:
the reference numbers in the drawings of the specification include: the device comprises a reaming drill bit 1, a drainage hole 101, a drill rod 2, a drill rod seal 201, a collecting pipe 3, a water outlet 301, a water inlet 302, a mixing and stirring tank 4, an inner tank 401, an outer tank 402, a mixing and stirring device 403, a turbulence device 404, a waste liquid collecting tank 5, a speed regulating motor 6, a locking and clamping cover 7 and a sleeve 8.
The embodiment is substantially as shown in fig. 1, 2 and 3: a marrow suction enrichment system comprises a marrow expanding drill bit 1, a drill rod 2, a collecting pipe 3, a mixed stirring tank 4 and a waste liquid collecting tank 5, wherein the marrow expanding drill bit 1 is detachably connected with the drill rod 2, in the embodiment, the marrow expanding drill bit 1 and the drill rod 2 are both hollow, the marrow expanding drill bit 1 is conical in the embodiment, and a plurality of drainage holes 101 which are arranged in an eccentric negative angle are formed in the marrow expanding drill bit 1; meanwhile, the reaming bit 1 is a replaceable bit, the specification range of the reaming bit 1 is 10mm-13mm, in the embodiment, every 0.5mm of the reaming bit 1 is provided with one specification, namely, 7 specifications of 10mm, 10.5mm, 11mm, 11.5mm, 12mm, 12.5mm and 13mm, and the reaming bit 1 can be applied to reaming under different conditions.
A double-helix structure is arranged on the drill rod 2, the collecting pipe 3 wraps the drill rod 2 and is used for collecting osteogenic materials, in the embodiment, the drill rod 2 is designed into a double-helix structure, can generate local negative pressure through high-speed rotation, is beneficial to unidirectional leading-out of osteogenic materials, is provided with a protection device at the double-helix position of the drill rod 2, is clamped with the collecting pipe 3, used for preventing the double helix structure of the drill rod 2 from scratching the inner wall of the collecting pipe 3, the protective device is a sleeve 8, one end of the sleeve 8 is provided with an external thread structure, the inner wall of the collecting pipe 3 is provided with an internal thread structure matched with the external thread of the sleeve 8, and the screwing direction of the external thread and the internal thread is the same as the rotating direction of the drill rod 2, so that the anti-screwing-off and anti-falling function can be realized, meanwhile, the other end of the sleeve 8 positioned on the external thread is provided with 4 holes for avoiding the problem that the osteogenic material enters the collecting pipe 3 unsmoothly.
The rear end of the drill rod 2 is provided with a driving mechanism, the driving mechanism is connected with the drill rod 2 in a sliding mode, the drill rod is located on a rotating shaft of a speed regulating motor, in the embodiment, the driving mechanism is a speed regulating motor 6, the rotating speed of the reaming bit 1 can be adjusted according to actual conditions, the speed regulating motor 6 is connected with the drill rod 2 in a sliding mode, and in the embodiment, the drill rod is sleeved and locked through a slidable locking and clamping cover 7.
As shown in fig. 4, a water outlet 301 and a water inlet 302 are arranged at the bottom of the rear end of the collecting pipe 3, the water inlet 302 is communicated with the tail of the drill rod 2, the water inlet 302 fills the physiological saline into the drill rod 2 through a pipeline, a drill rod seal 201 is arranged at the contact part of the speed regulating motor 6 and the drill rod 2, and the drill rod seal 201 is used for sealing the tail of the drill rod 2 when the drill rod 2 and the speed regulating motor 6 are locked by the locking clamp cover 7, so that the physiological saline is prevented from overflowing from the rear of the hollow drill rod 2; the water outlet 301 is used for discharging the collected bone raw material mixed liquid, the water outlet 301 is communicated with the mixing stirring tank through a pipeline, in the embodiment, a plastic pipeline is collected in the pipe 3, and the pipeline used by the water outlet 301 and the water inlet 302 is a colloid pipeline.
As shown in fig. 5 and 6, the mixing and stirring tank 4 comprises an outer tank 402 and an inner tank 401, a negative pressure suction device is arranged between the outer tank 402 and the inner tank 401, in the embodiment, the negative pressure suction device comprises an electromagnetic valve, and a power source of the negative pressure suction device adopts a negative pressure machine equipped in an operating room, so that the structure and the cost of the device can be reduced; the inner tank 401 contains an enrichment material and mixing and stirring device 403, a water outlet 301 is communicated with the outer tank 402 through a pipeline, and the bottom of the inner tank 401 is also provided with a flow disturbing device 404; the enrichment material in the inner canister 401 is an allogeneic bone decalcified scaffold, the surface of which is modified by self-assembly polypeptides, in this embodiment, the self-assembly polypeptides used comprise RADA16-I peptide and osteoprotetin peptide coupled to the C-terminus of the RADA16-I peptide sequence.
Mix agitating unit 403 and include (mixing) shaft and stirring leaf, the stirring leaf is located the (mixing) shaft, and (mixing) shaft one end is connected with vortex device 404, and the other end is connected with external drive arrangement, and in this embodiment, external drive arrangement does not need additionally to increase external source energy and intervenes for the power supply that adopts in the operating room, and stability and the security of assurance equipment that like this can be better, more operating rooms that are suitable for the difference require, reduce the cost simultaneously.
The turbulence device 404 is a propeller blade, and rotates through a stirring shaft, and the propeller blade is mainly used for enabling the osteogenic material deposited at the bottom of the inner tank 401 to flow, so that more substances required by enrichment of the enrichment material can be obtained.
Waste liquid collecting tank 5 is used for collecting the waste liquid that mixes in the stirring jar 4, mixes the bottom of the internal tank body 401 of stirring jar 4 and still is equipped with liquid outlet and filter equipment, and filter equipment is the filter screen in this embodiment, covers the liquid outlet, and the liquid outlet passes through pipeline and 5 intercommunications of waste liquid collecting tank.
The specific implementation process comprises the following steps: before the bone marrow suction enrichment system is used, firstly, physiological saline is injected into the drill rod 2 through the water inlet 302, then the locking clamping cover 7 is adjusted, the speed regulating motor 6 is locked with the drill rod 2, the drill rod seal 201 plugs the tail part of the drill rod 2 to prevent the physiological saline from overflowing, the speed regulating motor 6 is started to control the marrow expanding drill bit 1 and the drill rod 2 to carry out marrow expansion, the drainage hole 101 on the marrow expanding drill bit 1 discharges the physiological saline, the double-helix structure on the drill rod 2 generates local negative pressure in the high-speed rotation process to promote the unidirectional discharge of substances, the collection pipe 3 sleeved on the drill rod 2 collects the discharged substance and physiological saline mixed solution, the mixed solution flows into the outer tank body 402 of the mixing and stirring tank 4 through the water outlet 301 and the water outlet 301 pipeline, the negative pressure pipeline of the negative pressure suction system in an operating room is connected with an electromagnetic valve in the inner tank body 401, so that the negative pressure flowing into the outer tank body 402 is sucked into the inner tank body 401, the enrichment material that is arranged in the inner tank body 401 is enriched with the osteogenic material in the mixed liquid, and fully enriched under the action of the mixing and stirring device 403 and the turbulence device 404, so that the required osteogenic material can be collected in the collection process, the waste liquid generated after the collection is completed is filtered and discharged through the liquid outlet and the filter screen at the bottom of the inner tank body 401, and the filtered waste liquid is discharged into the waste liquid collection tank 5.
As shown in fig. 7, in another embodiment of the present invention, a method for constructing a high-activity bone based on a bone marrow aspiration enrichment system is further included, which specifically includes:
constructing an osteoinductive membrane: bone cement is used to fill the bone defect part to form a bone induction membrane.
In this embodiment, when bone cement is used to fill a bone defect, fibrin and laminin, etc. are rapidly enriched on the surface of the bone cement to form a membranous structure similar to periosteum, i.e. a bone inducing membrane, the inner layer of which is an epithelioid synovium, and the outer layer of which is composed of fibroblasts, myofibroblasts and type i collagen, and has a certain mechanical strength, and after the bone cement is taken out, a closed cavity capable of maintaining the autologous form can be formed, so that the tissue form of the transplanted bone can be maintained, the growth of surrounding soft tissues and bacterial invasion can be blocked, and the transplanted bone can be protected from being absorbed and the bone healing can be promoted.
Preparing an enriched material: and (3) placing the allogeneic bone decalcified scaffold into a prepared peptide solution for soaking, and washing after soaking to obtain the self-assembly polypeptide modified allogeneic bone decalcified scaffold.
In this embodiment, firstly, the allogeneic bone decalcified scaffold is taken out, and placed in a peptide solution for soaking, wherein the peptide solution is prepared by dissolving fusion peptide with a purity of 95% or more in a 20% sucrose solution until the final concentration of the solution is 1%, and performing ultrasonic treatment for 30 minutes to obtain a peptide solution; and then soaking the allogeneic bone decalcified scaffold in a peptide solution for 4-8 minutes, taking out the allogeneic bone decalcified scaffold, and washing the allogeneic bone decalcified scaffold with a phosphate buffer solution with a pH value of 7.4 to obtain the self-assembled polypeptide modified allogeneic bone decalcified scaffold.
An enrichment step: and placing the prepared enrichment material in an inner tank body of a mixing and stirring tank of the bone marrow suction enrichment system, and enriching according to preset time.
In this embodiment, place the allogeneic bone decalcification support of self-assembling polypeptide modification in the inner tank body of the compounding agitator tank of bone marrow suction enrichment system, constantly stir and the constantly disturbed flow effect of propeller blade through the (mixing) shaft, enrich the osteogenic material according to predetermined 30 minutes, required mesenchymal stem cell of absorption and required material that can be abundant.
Constructing high-activity bones: and discharging waste liquid in the mixing and stirring tank after the enrichment is finished to obtain the constructed high-activity bone repair material.
In this embodiment, after the enrichment is accomplished, discharge the waste liquid through filter equipment and the liquid outlet of the outer jar of body of compounding agitator tank, take out the inner tank body, obtain required high activity bone osteogenic material.
Repairing: taking out the bone cement, and implanting the constructed high-activity bone repair material into an induction membrane of a bone defect part.
In the embodiment, the time for forming the bone induction membrane is 8 weeks, the bone cement is taken out after the formation of the bone induction membrane, and the high-activity bone repair material is implanted into the bone defect part, wherein the bone induction membrane is a highly vascularized tissue with the thickness of 1-2 mm and consists of I-type colloid cells, and fibroblasts are the main cell types of the bone induction membrane; the inner surface of the membrane consists of epithelial-like cells, fibroblasts, myofibroblasts and collagen fiber bundles parallel to the surface of the membrane, and the bone induction membrane contains high-concentration vascular endothelial growth factors, transforming growth factors beta 1, bone morphogenetic protein-2 and other growth factors and can promote the regeneration and repair of bones. The membrane protein extract also has the property of stimulating the proliferation of bone marrow cells and the differentiation of mesenchymal cell membranes. Therefore, the bone induction membrane has a similar structure with the periosteum, has richer blood supply and richer active factors necessary for bone growth than the periosteum, and provides good biological conditions for bone healing for reconstructing bone defects.
It should be noted that, in this document, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or system that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or system. The term "comprising", without further limitation, means that the element so defined is not excluded from the group of processes, methods, articles, or systems that include the element.
The foregoing are merely exemplary embodiments of the present invention, and no attempt is made to show structural details of the invention in more detail than is necessary for the fundamental understanding of the art, the description taken with the drawings making apparent to those skilled in the art how the several forms of the invention may be embodied in practice with the teachings of the invention. It should be noted that, for those skilled in the art, without departing from the structure of the present invention, several changes and modifications can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicability of the patent. The scope of the claims of the present application shall be determined by the contents of the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.
Claims (10)
1. A bone marrow aspiration enrichment system, characterized in that: including reaming marrow drill bit, drilling rod, collecting pipe and mixing agitator tank, reaming marrow drill bit can be dismantled with the drilling rod and be connected, reaming marrow drill bit is hollow with the drilling rod, reaming marrow drill bit is equipped with the drainage hole that a plurality of is eccentric negative angle setting, the drilling rod rear end is equipped with actuating mechanism, actuating mechanism and drilling rod sliding connection, collecting pipe parcel drilling rod, the collecting pipe rear end is equipped with water inlet and delivery port, water inlet and drilling rod afterbody intercommunication, the compounding agitator tank includes the outer jar of body and the inner tank body, be equipped with negative pressure suction device between the outer jar of body and the inner tank body, contain enrichment material and compounding agitating unit in the inner tank body, the delivery port passes through pipeline and outer jar of body intercommunication, inner tank body bottom is equipped with the vortex device.
2. A bone marrow aspiration enrichment system according to claim 1, characterized in that: the enrichment material is an allogeneic bone decalcification support, and the surface of the allogeneic bone decalcification support is modified by self-assembly polypeptide.
3. The bone marrow suction and enrichment system as claimed in claim 1, characterized in that the mixing and stirring device comprises a stirring shaft and stirring blades, wherein the stirring blades are positioned on the stirring shaft, one end of the stirring shaft is connected with the flow disturbing device, and the other end of the stirring shaft is connected with an external driving device.
4. A bone marrow aspiration enrichment system according to claim 1, characterized in that: the flow disturbing device is a propeller blade.
5. A bone marrow aspiration enrichment system according to claim 1, characterized in that: the drill rod is provided with a double-spiral structure, and a collecting pipe at the double-spiral position of the drill rod is clamped with a protection device.
6. A bone marrow aspiration enrichment system according to claim 1, characterized in that: still include the waste liquid collecting tank, jar body bottom is equipped with liquid outlet and filter equipment in the mixing and stirring jar, the waste liquid collecting tank passes through the pipe connection liquid outlet.
7. A bone marrow aspiration enrichment system according to claim 1, characterized in that: the reaming bit is a replaceable reaming bit, and the specification of the reaming bit is 10mm-13 mm.
8. A bone marrow aspiration enrichment system according to claim 1, characterized in that: the driving mechanism is a speed regulating motor, and the drill rod is positioned on a rotating shaft of the speed regulating motor.
9. A high-activity bone construction method based on a bone marrow aspiration enrichment system is characterized in that: the method comprises the following steps:
preparing an enriched material: placing the allogeneic bone decalcification support into a prepared peptide solution for soaking, and washing after soaking to obtain the allogeneic bone decalcification support modified by the self-assembly polypeptide;
an enrichment step: placing the prepared enrichment material in an inner tank body of a mixing and stirring tank of a bone marrow suction enrichment system, and enriching according to preset time;
constructing high-activity bones: and discharging waste liquid in the mixing and stirring tank after the enrichment is finished to obtain the constructed high-activity bone repair material.
10. The method for constructing high-activity bone based on bone marrow aspiration enrichment system as claimed in claim 9, wherein: the step of preparing the enriched material further comprises the following steps of:
constructing an osteoinductive membrane: filling the bone defect part with bone cement to form a bone induction membrane;
the step of constructing the high-activity bone further comprises the following steps:
repairing: taking out the bone cement, and implanting the constructed high-activity bone repair material into an induction membrane of a bone defect part.
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| CN108025113A (en) * | 2015-07-31 | 2018-05-11 | 德普伊新特斯产品公司 | The method for preparing osteogenic bone graft |
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| JP2021094270A (en) * | 2019-12-18 | 2021-06-24 | 株式会社ネクスト２１ | Bone marrow collection device |
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| US6332886B1 (en) * | 1999-02-03 | 2001-12-25 | Synthes (Usa) | Surgical reamer and method of using same |
| US20030097133A1 (en) * | 2001-11-21 | 2003-05-22 | Green James M. | Attachable/detachable reaming head for surgical reamer |
| CN108025113A (en) * | 2015-07-31 | 2018-05-11 | 德普伊新特斯产品公司 | The method for preparing osteogenic bone graft |
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