CN114258811B - Method for inducing aquilaria sinensis to knot incense by utilizing high-pressure gas and application of method - Google Patents
Method for inducing aquilaria sinensis to knot incense by utilizing high-pressure gas and application of method Download PDFInfo
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Abstract
The invention discloses a method for inducing aquilaria sinensis to knot incense by utilizing high-pressure gas and application thereof. The method comprises the following steps: (1) manufacturing an inflator: drilling a hole on an agilawood trunk, winding a film on the outer side of one end of a steel pipe, inserting the film into the hole, winding the film at the joint of the steel pipe and the trunk, coating a sealant in a gap between the steel pipe and the tree hole and the joint of the trunk for sealing, connecting a rubber pipe at the other end of the steel pipe, and clamping the rubber pipe after the rubber pipe is folded by using a clamp; (2) high-pressure inflation treatment: loosening the clamp on the rubber tube, filling high-pressure gas into the hole on the aquilaria sinensis tree trunk through the rubber tube, and inflating once every 7-15 days for 6-12 times; wherein the high-pressure gas is at least one of ethylene, nitrogen and carbon dioxide. The invention utilizes gaseous substances to promote the gas conduction and physiological and biochemical reaction in the tree body in a high-pressure mode, shortens the induction time of the agilawood formation, and produces the agilawood with higher contents of chromone, sesquiterpene and other substances.
Description
Technical Field
The invention belongs to the technical field of biological agriculture, and particularly relates to a method for inducing aquilaria sinensis to knot fragrance by utilizing high-pressure gas and application thereof.
Background
Aquilaria sinensis (lour.) Gilg, an evergreen tree of the genus Aquilaria of the family Thymelaeaceae (Thymelaeaceae), is naturally distributed in provinces such as Hainan, guangdong, guangxi, fujian, taiwan, etc. Aquilaria sinensis is a secondary metabolite produced when Aquilaria plants are stimulated or damaged, and is widely applied to the aspects of medicine, spice, religion, collection and the like. Modern medical research shows that the agilawood has the effects of reducing blood pressure, tranquilizing, relieving pain, resisting arrhythmia, inhibiting excitation of a central system and the like. The agilawood is also a rare natural spice and rich in volatile oil components so that the agilawood is rich in fragrance.
Due to high economic value and huge market demand of the agilawood and a large amount of predatory logging and agilawood collection, wild agilawood resources in China are seriously damaged, and the wild agilawood resources are nearly exhausted. The native aquilaria wood is required to undergo a long external random interference process, and the process has the characteristics of contingency and long period. In recent years, people can induce the agilawood to produce incense to a certain extent by using methods such as physical injury, chemical reagent treatment or fungus treatment, but the content of alcohol-soluble extract and main chemical components (such as sesquiterpenes and chromone compounds) of the induced agilawood is generally low, and the quantity and the quality of the incense are required to be improved.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provides a method for inducing aquilaria sinensis to knot fragrance by utilizing high-pressure gas.
The invention also aims to provide application of the method for inducing the aquilaria sinensis to generate fragrance by utilizing the high-pressure gas.
The purpose of the invention is realized by the following technical scheme:
a method for inducing aquilaria sinensis to generate fragrance by utilizing high-pressure gas comprises the following steps:
(1) Manufacturing an inflating device:
drilling a hole on an agilawood trunk, winding a film on the outer side of one end of a steel pipe, inserting the film into the hole, winding the film at the joint of the steel pipe and the trunk, coating a sealant in a gap between the steel pipe and the tree hole and the joint of the trunk for sealing, connecting a rubber pipe at the other end of the steel pipe, and clamping the rubber pipe after the rubber pipe is folded by using a clamp;
(2) High-pressure inflation treatment:
loosening the clamp on the rubber tube, filling high-pressure gas into the hole on the agilawood trunk through the rubber tube, and inflating once every 7-15 days for 6-12 times; wherein the high-pressure gas is at least one of ethylene, nitrogen and carbon dioxide.
The aquilaria sinensis in the step (1) is grown for more than or equal to 8 years in tree age and has a breast diameter of more than or equal to 10cm; preferably, the agilawood with the tree age of more than or equal to 8 years, the breast diameter of more than or equal to 10cm and the height of 50cm and the diameter of more than 12cm is selected; more preferably aquilaria sinensis with tree age of more than 8 years, diameter at breast height of more than 11cm and height of 50cm, and diameter of more than 13 cm.
The height of the aquilaria sinensis tree trunk during drilling in the step (1) is preferably 50cm (from the ground).
The drilling in the step (1) is preferably performed by using an electric drill.
The hole diameter of the drilled hole in the step (1) is 10mm, and the depth of the drilled hole is 8-10 cm (preferably 9-10 cm).
The steel pipe in the step (1) is a steel pipe with the diameter of 9-10 mm and the length of 9-10 cm; preferably a stainless steel tube having a diameter of 9 to 10mm and a length of 9 to 10 cm.
The length of the steel pipe inserted into the hole in the step (1) is 2-3 cm.
The film wound on the outer side of one end of the steel pipe in the step (1) is a polypropylene film and/or a waterproof sealing adhesive tape; preferably a polypropylene film tape and/or a waterproof sealing tape; further preferably a polypropylene film tape with the width of 3-5 cm and/or a waterproof sealing tape with the width of 15-20 mm; and the waterproof sealing adhesive tape with the width of 15-20 mm is further preferred, the number of winding turns is 4-6, the length of winding the waterproof sealing adhesive tape onto the steel pipe is 3-5 cm, the filling and sealing effects can be achieved, and the subsequent hanging of the sealant is facilitated.
The film used at the interface of the steel pipe and the trunk in the step (1) is a polypropylene film and/or a grafting film; preferably a polypropylene film strip and/or a grafted film strip; further preferably a polypropylene film belt and/or a grafting film belt with the width of 3-5 cm; and the grafting film belt with the width of 3-5 cm is further preferable, the winding number of turns is 4-10 turns (in a mode of overlapping layer by layer; 8-10 turns are preferable), and the length of the wound steel pipe is 0.5-1 cm, so that the steel pipe is tightly attached to the tree body, and gaps are reduced.
The sealant in the step (1) can adopt a conventional commercially available sealant, and is preferably a 'Kafft' powerful AB adhesive.
The rubber tube in the step (1) can be a rubber tube, a medical rubber tube and the like; the length and the inner diameter can be selected according to actual requirements; preferably a rubber tube or a medical rubber tube with the length of 25cm and the inner diameter of 8 mm.
The clamp in the step (1) can be selected according to actual needs, and only the rubber tube can be clamped; preferably an iron clamp.
The high-pressure gas filling in the step (2) is preferably carried out by adopting a high-pressure steel bottle or a high-pressure gas bottle; the high-pressure steel cylinder or the high-pressure gas cylinder is provided with a voltage divider, and the voltage divider is connected with a flow meter (the flow meter is connected with a rubber tube).
The pressure of the high-pressure inflation in the step (2) is 0.1MPa (voltage division of a voltage divider).
The volume of the high-pressure gas filled in the step (2) is 1-2 times of the total volume of the tree hole, the steel pipe outside the tree body and the (external) rubber pipe; preferably 24 to 48mL.
The ethylene in the step (2) is ethylene with the purity of more than or equal to 99.0 percent; preferably, the purity of the ethylene is more than or equal to 99.9 percent.
The nitrogen in the step (2) is nitrogen with the purity of more than or equal to 99.0 percent; preferably nitrogen with the purity of more than or equal to 99.9 percent
The carbon dioxide in the step (2) has the purity of more than or equal to 99.0 percent; carbon dioxide with a purity of more than or equal to 99.9 percent is preferred.
The total time of the high pressure aeration treatment in the step (2) is preferably 3 to 6 months.
The method for inducing the aquilaria sinensis to knot fragrance by utilizing the high-pressure gas further comprises the following steps after the step (2): and after 9-12 months of treatment (continuous treatment), cutting black or brown greasy xylem formed around the hole, and drying to obtain the agilawood.
The drying is drying.
The method for inducing the aquilaria sinensis to generate the incense by utilizing the high-pressure gas is applied to inducing the aquilaria sinensis to generate the incense.
Compared with the prior art, the invention has the following advantages and effects:
(1) The invention utilizes gaseous substances to promote gas conduction and physiological and biochemical reaction in the tree body by adopting a high-pressure mode, overcomes the defects of leaf fall and core rot of the tree body and the like caused by utilizing liquid chemical and biological induction methods, shortens the fragrance-forming induction time, leads the trunk to carry out transverse fragrance-forming, and produces the agilawood with hard texture, rich fragrance, high content of main contents and high content of grease.
(2) The method for inducing the formation of the agilawood has the advantages of low cost, easy operation, strong practicability, long longitudinal formation of the agilawood, deep transverse formation and large formation area, and is suitable for vast aquilaria sinensis growers; the produced agilawood is simulated to form natural agilawood, does not contain chemical pollutants, is close to the quality of the natural Kyara and has the linalool extract content higher than the national pharmacopoeia standard.
(3) Compared with the traditional physical incense forming method, the induction method of the invention has the advantages of fast incense forming and deep incense forming area; compared with the chemical induction method for producing the fragrant, the induction method does not cause fallen leaves and yellow leaves of the tree body and influence the growth and development of the tree; compared with fungus induced incense producing method, the method does not cause core rot and hollow phenomenon, and the produced agilawood has no mixed bacteria pollution phenomenon.
(4) The agilawood induced by the method is compact and hard in texture and strong in fragrance, and compared with the existing liquid chemical and biological induction methods, the content of substances such as chromone, sesquiterpene and the like in the produced agilawood is higher.
Drawings
FIG. 1 is a view showing a process of winding a thin film on one end of a steel pipe in example 1 of the present invention.
FIG. 2 is a view showing the clamping process of the rubber tube folding iron clamp in example 1 of the present invention.
Fig. 3 is a diagram of the trunk sealing process in example 1 of the present invention.
FIG. 4 is a diagram of an operation in the manual high-pressure inflation site in example 1 of the present invention.
Detailed Description
The present invention will be described in further detail with reference to examples, but the embodiments of the present invention are not limited thereto. Reagents, methods and apparatus used in the present invention are conventional in the art unless otherwise indicated.
The method for measuring the contents of alcohol-soluble substances (alcohol-soluble extracts), sesquiterpenes and chromone substances in the embodiment of the invention comprises the following steps:
(1) The content of alcohol-soluble extract in the linaloe oil is detected by a hot melting method: the measurement was carried out by the hot melt method in accordance with the method of Chinese pharmacopoeia (first part of 2010). The specific method comprises the following steps: the sample is placed in a 40 ℃ constant temperature air blast drying oven, taken out and crushed after 48 hours, 2g of powder is accurately weighed and placed in a 100mL conical flask, 50mL of ethanol is added, a plug is added, and the mixture is weighed and then stands for 1 hour. After the conical flask is connected with a reflux condenser tube, the conical flask is heated to boiling and slightly boiled for 1 hour. After cooling, taking down the conical flask, adding a plug, weighing, calculating the weight loss reduction amount, adding ethanol with the same amount as the weight loss reduction amount, and shaking up. Taking 1/2 of the filtrate, placing the filtrate in an evaporating dish which is dried to constant weight, evaporating the ethanol in a water bath, drying the filtrate for 2 hours at 105 ℃, placing the filtrate in a dryer, cooling the filtrate, and quickly taking out and weighing the filtrate. The weight of the dried product is the content of ethanol extract in each gram of sample.
(2) The method for detecting the content of sesquiterpenes and chromones in the linaloe oil comprises the following steps: GC-MS analysis was performed on the ethanol extracted linaloe oil. A gas chromatograph-mass spectrometer (Agilent 7890B-5977A, USA) is used. GS-MS conditions: column HP-5MS (30 m.times.0.25 mm.times.0.25 μm); the initial temperature is 70 ℃, the temperature is kept for 1min, then the temperature is raised to 250 ℃ at the speed of 8 ℃/min, and the temperature is kept for 10min; the temperature of a sample inlet is 250 ℃; the sample volume was 0.5 μ L (no split); the interface temperature of the chromatograph-mass spectrometer is 280 ℃; the ion source temperature is 230 ℃; an ionization mode EI; electron energy 70e V; carrier gas He (99.999%); the flow rate of the carrier gas is 1.0mL/min; the mass scanning range is 35-350 AMU; the solvent was delayed for 4min. Calculating the content of each chemical component by HPMSD chemical workstation and peak area normalization, and performing artificial spectrogram analysis and computer retrieval by NIST and Willy standard mass spectrometry library.
The film used for winding the outer side of one end of the steel pipe in the embodiment of the invention can be a conventional commercially available polypropylene film and/or a waterproof sealing adhesive tape; preferably a polypropylene film tape with the width of 3-5 cm and/or a waterproof sealing adhesive tape with the width of 15-20 mm; more preferably 15 to 20 mm.
The film used at the interface of the steel pipe and the trunk can be a conventional commercially available polypropylene film or a grafted film; preferably a polypropylene film belt or a grafting film belt with the width of 3-5 cm; more preferably 3 to 5cm of grafted film tape.
Example 1
1. Inflator preparation
Selecting 7 months sunny days, selecting 8-year-old eaglewood trees with the average diameter at breast height of 11.3cm, drilling holes at the position of 50cm (the average diameter is 13.2 cm) of the trunk height by using an electric drill, wherein the diameter of each hole is 10mm, and the length of each hole is 10cm; winding a waterproof sealing adhesive tape (the waterproof sealing adhesive tape with the width of 15-20 mm) on the outer side of one end of a steel pipe (the diameter of 10 mm) with the length of 9cm, wherein the length of the wound steel pipe is 3-5 cm, the waterproof sealing adhesive tape can play a role in filling and sealing, is also favorable for hanging sealant and is wound for 4-6 circles, and then inserting the wound steel pipe into a tree hole with the depth of 2-3 cm; the other end of the steel pipe (namely the steel pipe outside the trunk hole) is connected with a rubber pipe (the rubber pipe is sleeved outside the steel pipe, and the inner diameter of the rubber pipe is 8 mm) with the length of 25 cm; the joint of the steel pipe and the trunk is sealed by film winding (winding by a grafting film belt of 3-5 cm layer by layer), and the steel pipe is wound for 8-10 circles, and the length of the wound steel pipe is 0.5-1 cm, so that the steel pipe is tightly attached to the trunk, and gaps are reduced; then coating the outside with a commercial 'Kafter' strong AB glue for strengthening and sealing. The rubber tube outside the tree is clamped and sealed by an iron clamp after being folded (figures 1-4).
2. High pressure inflation
High-pressure gas with purity of 99.0 percent of ethylene, 99.9 percent of nitrogen and 99.9 percent of carbon dioxide is selected. When the gas is filled, an iron clamp on the rubber tube (namely the rubber tube) is opened, a valve of the high-pressure steel cylinder is opened, the processing gas in the high-pressure steel cylinder enters the flowmeter after being divided by the voltage divider, and then is injected into the tree hole through the rubber tube outside the tree body, and the tree hole is sealed in time. The gas volume charged was 24mL (pressure 0.1 MPa). The aeration treatment was carried out once every 7 days for 12 times.
3. Comparison method
The method comprises the following steps: high pressure ethylene gas induction.
The second method of the invention: high pressure nitrogen induction.
The third method of the invention: high pressure carbon dioxide gas induction.
The comparison method comprises the following steps: only holes are punched, and the air is not inflated.
4. Detection of
When the treatment is completed for 9 months (namely after 12 times of treatment, the treatment is carried out for 9 months), the black or brown greasy xylem formed around the air charging hole is cut, the treatment is carried out according to the 2017 forestry industry standard method, and the contents of alcohol-soluble extracts, sesquiterpenes and chromone substances are measured (see table 1).
TABLE 1 lignum Aquilariae Resinatum quality detection Table
| Treatment of | Alcohol-soluble substance content (%) | Sesquiterpene substance content (%) | Chromone content (%) |
| The method of the invention 1 | 17.87 | 15.5 | 41.65 |
| Method two of the invention | 19.03 | 23.1 | 24.66 |
| Method III of the invention | 19.81 | 14.86 | 25.65 |
| Comparison method | 2.93 | 0.29 | 0 |
Example 2
1. Inflator preparation
Selecting a sunny day of 9 months, selecting 8-year-old eaglewood trees with the average diameter at breast height of 12.5cm, electrically drilling holes with the diameter of 10mm and the length of 10cm at the position 50cm (the average diameter of 14.06 cm) of the trunk height; winding a waterproof sealing adhesive tape (the waterproof sealing adhesive tape with the width of 15-20 mm) on the outer side of one end of a steel pipe (the diameter of 10 mm) with the length of 9cm, wherein the length of the wound steel pipe is 3-5 cm, the waterproof sealing adhesive tape can play a role in filling and sealing, is also favorable for hanging sealant and is wound for 4-6 circles, and then inserting the wound steel pipe into a tree hole with the depth of 2-3 cm; the other end of the steel pipe (namely the steel pipe outside the trunk hole) is connected with a rubber pipe (the rubber pipe is sleeved outside the steel pipe; the inner diameter of the rubber pipe is 8 mm) with the length of 25cm, the interface of the steel pipe and the trunk is sealed by a film winding (a grafting film belt with the length of 3-5 cm is wound layer by layer) for 8-10 circles, and the length of the wound steel pipe is 0.5-1 cm, so that the steel pipe is tightly attached to the trunk body, and gaps are reduced; finally, the strong AB glue of the commercial Kafter is used for strengthening and sealing. The rubber tube outside the tree is clamped and sealed by an iron clamp after being folded.
2. High pressure inflation
High-pressure gas with purity of 99.0 percent of ethylene, 99.9 percent of nitrogen and 99.9 percent of carbon dioxide is selected. When the gas is filled, the iron clamp on the rubber tube is opened, the valve of the high-pressure steel cylinder is opened, the processing gas in the high-pressure steel cylinder enters the flowmeter after being divided by the voltage divider, and then is injected into the tree hole through the rubber tube outside the tree body, and the tree hole is sealed in time. The gas volume charged was 24mL (0.1 MPa). Inflating once every 15 days for 6 times (namely 3 months)
3. Comparison method
The first method of the invention: high pressure ethylene gas induction.
The second method of the invention: high pressure nitrogen induction.
The third method of the invention: high pressure carbon dioxide gas induction.
The comparison method comprises the following steps: only holes are punched, and the air is not inflated.
4. Detection of
When 9 months (i.e. after 3 months of treatment, and then 9 months), samples were taken to determine the alcohol-soluble extract, sesquiterpene and chromone content (see Table 2).
TABLE 2 lignum Aquilariae Resinatum quality detection Table
| Treatment of | Alcohol-soluble substance content (%) | Sesquiterpene substance content (%) | Chromone content (%) |
| The method of the invention 1 | 17.03 | 10.72 | 39.03 |
| Method two of the invention | 15.72 | 13.02 | 20.17 |
| Method III of the invention | 15.26 | 18.66 | 20.46 |
| Comparison method | 2.93 | 0.29 | 0 |
Example 3
1. Inflator preparation
Selecting 11 months sunny days, selecting a 10-year-old eaglewood tree with an average diameter at breast height of 13.15cm, and drilling a hole with a diameter of 10mm and a length of 10cm at a position with a trunk height of 50cm (average diameter of 14.33 cm) by using an electric drill. The method comprises the following steps of winding a waterproof sealing adhesive tape (the waterproof sealing adhesive tape with the width of 15-20 mm) on the outer side of one end of a steel pipe (the diameter of 10 mm) with the length of 9cm (the length of the wound steel pipe is 3-5 cm, the effect of filling and sealing can be achieved, hanging sealant is facilitated, the steel pipe is wound for 4-6 circles), then the steel pipe is inserted into a tree hole, the depth is 2-3 cm, the other end of the steel pipe (namely the steel pipe outside the tree hole) is connected with a rubber pipe (the rubber pipe is sleeved on the outer side of the steel pipe, the inner diameter of the rubber pipe is 8 mm), a grafting film is sealed at the winding interface of the steel pipe and the tree trunk (the grafting film tape with the length of 3-5 cm is wound layer by layer), the steel pipe is wound for 8-10 circles, and the length of the wound steel pipe is 0.5-1 cm, so that the steel pipe and the tree body are tightly attached to reduce gaps; finally, the strong AB glue of the commercial Kafter is used for strengthening and sealing. The rubber tube outside the tree is clamped and sealed by an iron clamp after being folded.
2. High pressure inflation
High-pressure gas with purity of 99.0 percent of ethylene, 99.9 percent of nitrogen and 99.9 percent of carbon dioxide is selected. When the gas is filled, the iron clamp on the rubber tube is opened, the valve of the high-pressure steel cylinder is opened, the processing gas in the high-pressure steel cylinder enters the flowmeter after being divided by the voltage divider, and then is injected into the tree hole through the rubber tube outside the tree body, and the tree hole is sealed in time. The gas volume charged was 48mL (0.1 MPa). The air was added every 7 days for 12 treatments.
3. Comparison method
The method comprises the following steps: high pressure ethylene gas induction.
The second method of the invention: high pressure nitrogen induction.
The third method of the invention: high pressure carbon dioxide gas induction.
The comparison method comprises the following steps: only holes are punched, and the air is not inflated.
4. Detection
At 9 months after the treatment (i.e. after 12 treatments, 9 months), samples were taken to determine the alcohol-soluble extract, sesquiterpene and chromone content (see Table 3).
TABLE 3 agilawood quality detection table
| Treatment of | Alcohol-soluble substance content (%) | Sesquiterpene substance content (%) | Chromone content (%) |
| The method of the invention 1 | 20.78 | 18.40 | 44.85 |
| Method two of the invention | 19.01 | 26.32 | 27.85 |
| Method III of the invention | 21.14 | 21.15 | 28.41 |
| Comparison method | 2.84 | 0.27 | 0 |
The data in tables 1-3 show that the invention not only increases the content of the alcohol-soluble substances in the xylem of the aquilaria sinensis, but also greatly increases the content of the sesquiterpenes and chromones.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (9)
1. A method for inducing Aquilaria sinensis to generate fragrance by using high-pressure gas is characterized by comprising the following steps:
(1) Manufacturing an inflating device:
drilling a hole on an agilawood trunk, winding a film on the outer side of one end of a steel pipe, inserting the film into the hole, winding the film at the joint of the steel pipe and the trunk, coating a sealant in a gap between the steel pipe and the tree hole and the joint of the trunk for sealing, connecting a rubber pipe at the other end of the steel pipe, and clamping the rubber pipe after the rubber pipe is folded by using a clamp;
(2) High-pressure inflation treatment:
loosening the clamp on the rubber tube, filling high-pressure gas into the hole on the agilawood trunk through the rubber tube, and inflating once every 7-15 days for 6-12 times; wherein the high-pressure gas is at least one of ethylene, nitrogen and carbon dioxide;
the aquilaria sinensis in the step (1) is grown for more than or equal to 8 years in tree age and has a breast diameter of more than or equal to 10cm;
the diameter of the drilled hole in the step (1) is 10mm, and the depth of the drilled hole is 8-10 cm;
the steel pipe in the step (1) is a steel pipe with the diameter of 9-10 mm and the length of 9-10 cm;
the length of the steel pipe inserted into the hole in the step (1) is 2-3 cm;
the volume of the high-pressure gas filled in the step (2) is 1-2 times of the total volume of the tree hole, the steel pipe outside the tree body and the rubber pipe;
the pressure of the high-pressure inflation in the step (2) is 0.1MPa.
2. The method for inducing aquilaria sinensis to knot using high-pressure gas as claimed in claim 1, wherein:
the aquilaria sinensis in the step (1) is grown at the age of more than or equal to 8 years, the breast diameter is more than or equal to 10cm, and the height of 50cm is more than 12cm in diameter;
the diameter of the drilled hole in the step (1) is 10mm, and the depth of the drilled hole is 9-10 cm;
the steel pipe in the step (1) is a stainless steel pipe with the diameter of 9-10 mm and the length of 9-10 cm;
the volume of the high-pressure gas filled in the step (2) is 24-48 mL.
3. The method for inducing aquilaria sinensis to knot using high-pressure gas as claimed in claim 1, wherein:
the film wound on the outer side of one end of the steel pipe in the step (1) is a polypropylene film and/or a waterproof sealing adhesive tape;
the film used at the interface of the steel pipe and the trunk in the step (1) is a polypropylene film and/or a grafted film;
the rubber tube in the step (1) is a rubber tube, a rubber tube or a medical rubber tube.
4. The method for inducing aquilaria sinensis to knot using high-pressure gas as claimed in claim 3, wherein:
the film wound on the outer side of one end of the steel pipe in the step (1) is a waterproof sealing adhesive tape with the width of 15-20 mm, the number of winding turns is 4-6, and the length of the wound steel pipe is 3-5 cm;
the film used at the interface of the steel pipe and the trunk in the step (1) is a grafting film belt with the width of 3-5 cm, the number of winding turns is 4-10, and the length of the wound steel pipe is 0.5-1 cm;
the rubber tube in the step (1) is a rubber tube or a medical rubber tube with the length of 25cm and the inner diameter of 8 mm.
5. The method for inducing aquilaria sinensis to knot using high-pressure gas as claimed in claim 1, wherein:
the height of the aquilaria sinensis tree trunk during drilling in the step (1) is 50cm;
the total time of the high-pressure inflation treatment in the step (2) is 3-6 months.
6. The method for inducing aquilaria sinensis to knot using high-pressure gas as claimed in claim 1, wherein:
the ethylene in the step (2) has the purity of more than or equal to 99.0 percent;
the nitrogen in the step (2) is nitrogen with the purity of more than or equal to 99.0 percent;
the carbon dioxide in the step (2) has the purity of more than or equal to 99.0 percent.
7. The method for inducing aquilaria sinensis to knot using high-pressure gas as claimed in claim 1, wherein:
the sealant in the step (1) is a Kafft powerful AB adhesive;
the clamp in the step (1) is an iron clamp;
drilling in the step (1) by using an electric drill;
the high-pressure inflation in the step (2) is performed by adopting a high-pressure steel cylinder or a high-pressure gas cylinder; the high-pressure steel cylinder or the high-pressure gas cylinder is provided with a voltage divider, and the voltage divider is connected with the flowmeter.
8. The method for inducing aquilaria sinensis to knot using high-pressure gas as claimed in claim 1, further comprising the following steps after the step (2): after 9-12 months of treatment, cutting the black or brown greasy xylem formed around the hole, and drying to obtain the agilawood;
the drying is drying.
9. The use of the method for inducing aquilaria sinensis to generate incense by using high-pressure gas as claimed in any one of claims 1 to 8 in inducing aquilaria sinensis to generate incense.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1387821A (en) * | 1971-05-18 | 1975-03-19 | American Cyanamid Co | Producing chemical plant metabolites by suspension culture |
| CN101142890A (en) * | 2001-05-24 | 2008-03-19 | 明尼苏达大学董事会 | Cultivated agarwood |
| TW201814011A (en) * | 2016-09-20 | 2018-04-16 | 八毅國際股份有限公司 | Method for generating aquilaria resin capable of quickly generating the aquilaria resin while keeping the aquilaria tree to have a good appearance |
| TW202038706A (en) * | 2019-04-25 | 2020-11-01 | 八毅國際股份有限公司 | Method of producing agarwood resin can reduce probability for agarwood tree that is unable to convey nutrient |
| CN111903407A (en) * | 2019-05-09 | 2020-11-10 | 八毅国际股份有限公司 | Method for producing linaloe resin |
Family Cites Families (3)
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| CN105850543A (en) * | 2016-05-10 | 2016-08-17 | 中国医学科学院药用植物研究所海南分所 | Aquilaria sinensis high-pressure infusion tambac generating method and device |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1387821A (en) * | 1971-05-18 | 1975-03-19 | American Cyanamid Co | Producing chemical plant metabolites by suspension culture |
| CN101142890A (en) * | 2001-05-24 | 2008-03-19 | 明尼苏达大学董事会 | Cultivated agarwood |
| TW201814011A (en) * | 2016-09-20 | 2018-04-16 | 八毅國際股份有限公司 | Method for generating aquilaria resin capable of quickly generating the aquilaria resin while keeping the aquilaria tree to have a good appearance |
| TW202038706A (en) * | 2019-04-25 | 2020-11-01 | 八毅國際股份有限公司 | Method of producing agarwood resin can reduce probability for agarwood tree that is unable to convey nutrient |
| CN111903407A (en) * | 2019-05-09 | 2020-11-10 | 八毅国际股份有限公司 | Method for producing linaloe resin |
Non-Patent Citations (1)
| Title |
|---|
| 木材中树脂及树脂障碍的研究进展;李超等;《华东纸业》;20090831;第40卷(第04期);12-17 * |
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