CN114250008A - Biological cleaning agent capable of degrading printing ink coating and preparation method thereof - Google Patents
Biological cleaning agent capable of degrading printing ink coating and preparation method thereof Download PDFInfo
- Publication number
- CN114250008A CN114250008A CN202111673993.5A CN202111673993A CN114250008A CN 114250008 A CN114250008 A CN 114250008A CN 202111673993 A CN202111673993 A CN 202111673993A CN 114250008 A CN114250008 A CN 114250008A
- Authority
- CN
- China
- Prior art keywords
- parts
- weight
- biological
- cleaning agent
- bacillus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000012459 cleaning agent Substances 0.000 title claims abstract description 73
- 230000000593 degrading effect Effects 0.000 title claims abstract description 16
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 238000000576 coating method Methods 0.000 title claims description 32
- 239000011248 coating agent Substances 0.000 title claims description 30
- 238000007639 printing Methods 0.000 title description 8
- 108090000371 Esterases Proteins 0.000 claims abstract description 39
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 31
- 239000001509 sodium citrate Substances 0.000 claims abstract description 22
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims abstract description 22
- ZTOKUMPYMPKCFX-CZNUEWPDSA-N (E)-17-[(2R,3R,4S,5S,6R)-6-(acetyloxymethyl)-3-[(2S,3R,4S,5S,6R)-6-(acetyloxymethyl)-3,4,5-trihydroxyoxan-2-yl]oxy-4,5-dihydroxyoxan-2-yl]oxyoctadec-9-enoic acid Chemical compound OC(=O)CCCCCCC/C=C/CCCCCCC(C)O[C@@H]1O[C@H](COC(C)=O)[C@@H](O)[C@H](O)[C@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](COC(C)=O)O1 ZTOKUMPYMPKCFX-CZNUEWPDSA-N 0.000 claims abstract description 17
- 239000002736 nonionic surfactant Substances 0.000 claims abstract description 15
- 239000002904 solvent Substances 0.000 claims abstract description 15
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 14
- 239000002270 dispersing agent Substances 0.000 claims abstract description 14
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000003973 paint Substances 0.000 claims abstract description 11
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 39
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid group Chemical group C(CC(O)(C(=O)O)CC(=O)O)(=O)O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 36
- 239000006228 supernatant Substances 0.000 claims description 31
- 241001052560 Thallis Species 0.000 claims description 22
- 239000007788 liquid Substances 0.000 claims description 22
- AEQDJSLRWYMAQI-UHFFFAOYSA-N 2,3,9,10-tetramethoxy-6,8,13,13a-tetrahydro-5H-isoquinolino[2,1-b]isoquinoline Chemical group C1CN2CC(C(=C(OC)C=C3)OC)=C3CC2C2=C1C=C(OC)C(OC)=C2 AEQDJSLRWYMAQI-UHFFFAOYSA-N 0.000 claims description 21
- 238000000855 fermentation Methods 0.000 claims description 21
- 230000004151 fermentation Effects 0.000 claims description 21
- 239000000176 sodium gluconate Substances 0.000 claims description 21
- 229940005574 sodium gluconate Drugs 0.000 claims description 21
- 235000012207 sodium gluconate Nutrition 0.000 claims description 21
- 230000001580 bacterial effect Effects 0.000 claims description 20
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 17
- 229920001577 copolymer Polymers 0.000 claims description 17
- 229910052760 oxygen Inorganic materials 0.000 claims description 17
- 239000001301 oxygen Substances 0.000 claims description 17
- 238000003756 stirring Methods 0.000 claims description 16
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 14
- 229960001763 zinc sulfate Drugs 0.000 claims description 14
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 12
- 239000008367 deionised water Substances 0.000 claims description 12
- 229910021641 deionized water Inorganic materials 0.000 claims description 12
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 12
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 11
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 11
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 11
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 11
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 11
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 11
- 229960001484 edetic acid Drugs 0.000 claims description 11
- 229920001400 block copolymer Polymers 0.000 claims description 10
- 238000001816 cooling Methods 0.000 claims description 10
- 238000010438 heat treatment Methods 0.000 claims description 10
- 239000000725 suspension Substances 0.000 claims description 10
- 238000000108 ultra-filtration Methods 0.000 claims description 10
- 238000005406 washing Methods 0.000 claims description 10
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical group [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 claims description 9
- 229910052938 sodium sulfate Inorganic materials 0.000 claims description 9
- 235000011152 sodium sulphate Nutrition 0.000 claims description 9
- 239000012634 fragment Substances 0.000 claims description 8
- 238000011081 inoculation Methods 0.000 claims description 8
- 239000012528 membrane Substances 0.000 claims description 8
- 230000004936 stimulating effect Effects 0.000 claims description 8
- 241000194110 Bacillus sp. (in: Bacteria) Species 0.000 claims description 7
- 108090000790 Enzymes Proteins 0.000 claims description 6
- 102000004190 Enzymes Human genes 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 4
- 229940044197 ammonium sulfate Drugs 0.000 claims description 2
- 229910001871 ammonium zinc sulfate Inorganic materials 0.000 claims description 2
- 229960001790 sodium citrate Drugs 0.000 claims description 2
- 235000011083 sodium citrates Nutrition 0.000 claims description 2
- 230000000638 stimulation Effects 0.000 claims description 2
- 229940074410 trehalose Drugs 0.000 claims description 2
- 230000006698 induction Effects 0.000 claims 2
- 238000004140 cleaning Methods 0.000 abstract description 22
- 239000004094 surface-active agent Substances 0.000 abstract description 10
- 230000000694 effects Effects 0.000 abstract description 9
- 230000002195 synergetic effect Effects 0.000 abstract description 6
- 238000004945 emulsification Methods 0.000 abstract description 3
- 230000035699 permeability Effects 0.000 abstract description 3
- 230000032683 aging Effects 0.000 abstract description 2
- 230000015556 catabolic process Effects 0.000 abstract description 2
- 238000013329 compounding Methods 0.000 abstract description 2
- 238000006731 degradation reaction Methods 0.000 abstract description 2
- 230000003381 solubilizing effect Effects 0.000 abstract description 2
- 230000009044 synergistic interaction Effects 0.000 abstract description 2
- 150000001875 compounds Chemical class 0.000 abstract 1
- 235000019441 ethanol Nutrition 0.000 description 10
- 230000000052 comparative effect Effects 0.000 description 9
- 230000001954 sterilising effect Effects 0.000 description 9
- 238000012258 culturing Methods 0.000 description 8
- 230000001276 controlling effect Effects 0.000 description 7
- 239000000126 substance Substances 0.000 description 6
- 230000007613 environmental effect Effects 0.000 description 5
- 229940088598 enzyme Drugs 0.000 description 4
- 150000002632 lipids Chemical class 0.000 description 4
- 229910052751 metal Inorganic materials 0.000 description 4
- 239000002184 metal Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 238000011086 high cleaning Methods 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- SMZOUWXMTYCWNB-UHFFFAOYSA-N 2-(2-methoxy-5-methylphenyl)ethanamine Chemical compound COC1=CC=C(C)C=C1CCN SMZOUWXMTYCWNB-UHFFFAOYSA-N 0.000 description 2
- NIXOWILDQLNWCW-UHFFFAOYSA-N 2-Propenoic acid Natural products OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 2
- OFOBLEOULBTSOW-UHFFFAOYSA-N Propanedioic acid Natural products OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 description 2
- GOOHAUXETOMSMM-UHFFFAOYSA-N Propylene oxide Chemical compound CC1CO1 GOOHAUXETOMSMM-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000004519 grease Substances 0.000 description 2
- 239000008233 hard water Substances 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 description 2
- 239000011976 maleic acid Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 241000192125 Firmicutes Species 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- 102000016943 Muramidase Human genes 0.000 description 1
- 108010014251 Muramidase Proteins 0.000 description 1
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000003373 anti-fouling effect Effects 0.000 description 1
- 238000006065 biodegradation reaction Methods 0.000 description 1
- 239000006229 carbon black Substances 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000008139 complexing agent Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- GVGUFUZHNYFZLC-UHFFFAOYSA-N dodecyl benzenesulfonate;sodium Chemical compound [Na].CCCCCCCCCCCCOS(=O)(=O)C1=CC=CC=C1 GVGUFUZHNYFZLC-UHFFFAOYSA-N 0.000 description 1
- 230000001804 emulsifying effect Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000003822 epoxy resin Substances 0.000 description 1
- XZUAPPXGIFNDRA-UHFFFAOYSA-N ethane-1,2-diamine;hydrate Chemical compound O.NCCN XZUAPPXGIFNDRA-UHFFFAOYSA-N 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 239000002360 explosive Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 238000000265 homogenisation Methods 0.000 description 1
- 239000010720 hydraulic oil Substances 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 229960000274 lysozyme Drugs 0.000 description 1
- 239000004325 lysozyme Substances 0.000 description 1
- 235000010335 lysozyme Nutrition 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
- 239000000693 micelle Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000009972 noncorrosive effect Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000003002 pH adjusting agent Substances 0.000 description 1
- 229920000647 polyepoxide Polymers 0.000 description 1
- 229940051841 polyoxyethylene ether Drugs 0.000 description 1
- 229920000056 polyoxyethylene ether Polymers 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000007127 saponification reaction Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229940080264 sodium dodecylbenzenesulfonate Drugs 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000003981 vehicle Substances 0.000 description 1
- 239000003871 white petrolatum Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09D—COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR
- C09D9/00—Chemical paint or ink removers
- C09D9/04—Chemical paint or ink removers with surface-active agents
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09D—COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR
- C09D9/00—Chemical paint or ink removers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Materials Engineering (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Detergent Compositions (AREA)
Abstract
The invention discloses a biological cleaning agent capable of degrading ink paint and a preparation method thereof, wherein the cleaning agent comprises the following components: 0.02-0.07 part by weight of biological esterase solution; 0.06-0.09 parts by weight of sophorolipid; 4-10 parts of nonionic surfactant; 3-8 parts of a solubilizer; 9-12 parts of a dispersant; 0.03-0.09 part by weight of ethylenediamine tetraacetic acid; 0.01-0.02 weight part of sodium citrate; 0.02-0.06 parts of pH value regulator; 0.02-0.04 parts by weight of an anti-interference agent; and water. The biological cleaning agent is based on the compound compounding of biological esterase and a surfactant, has a synergistic interaction effect, can enhance the degradation force, the permeability and the emulsification effect of the cleaning agent, and achieves the aim of high-efficiency cleaning; the synergistic effect of sophorolipid and biological esterase can improve the solubilizing effect of the biological cleaning agent, improve the pollution resistance, improve the easy aging of the cleaning agent and the self-cleaning function of the solution brought by repeated use, and improve the repeated use frequency.
Description
Technical Field
The invention relates to the technical field of ink paint cleaning agents, in particular to a biological cleaning agent capable of degrading an ink paint and a preparation method thereof.
Background
The cleaning of the ink coating is commonly existed in the industrial and daily life fields, such as the cleaning of printing equipment, the cleaning of vehicle and ship surface coatings, the cleaning of special equipment maintenance and the like. The traditional ink cleaning method generally uses organic solvents which are flammable and explosive and have serious environmental pollution, and the use of the organic solvents is gradually forbidden in many countries and regions. Along with the technical progress and the environmental protection requirement, the water-based paint cleaning agent, especially the degradable environmental protection biological cleaning agent, has obvious progress, and the greatest characteristic of the biological paint cleaning agent is high efficiency and ultra-long service cycle, and can obtain better environmental protection safety.
The reality is that the homogenization problem of water-based products in the cleaning industry of China is serious. Most of domestic water-based cleaning agents are chemical cleaning agents formed by compounding surfactants and alkaline auxiliaries, strong alkaline cleaning agents still occupy a large market, the cleaning agents are simple in production process and low in cost, but easily cause the problems that personnel and equipment are damaged, and the like, and waste liquid after cleaning is difficult to treat.
Chinese patent publication No. CN102604754A (published: 20120725) discloses a biological enzyme cleaning agent, the main active components of which comprise biological enzyme liquid, sodium dodecyl benzene sulfonate, alkylphenol ethoxylates, fatty alcohol-polyoxyethylene ether, absolute ethyl alcohol, glycerol, lipase, amylase, cellulase, lysozyme and the like, wherein the biological enzyme liquid is a proteolytic enzyme prepared by deep fermentation, extraction and refining of bacillus subtilis. The biological enzyme cleaning agent has high cleaning rate and a certain sterilization effect, but is difficult to be applied to industrial cleaning in a large scale due to complex formula and high raw material price, and is more suitable for the fields of cleaning medical instruments with high sanitary and clean requirements and the like.
The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person skilled in the art.
Disclosure of Invention
The invention aims to provide a biological cleaning agent capable of degrading an ink coating, which has the advantages of high cleaning rate on the metal surface covered by the ink coating, low production cost, neutrality, mildness, environmental protection and safety, and can effectively promote the degradation of the ink.
In order to achieve the aim, the invention provides a biological cleaning agent capable of degrading an ink coating, which comprises the following components in parts by weight:
0.02-0.07 part by weight of biological esterase solution;
0.06-0.09 parts by weight of sophorolipid;
4-10 parts of nonionic surfactant;
3-8 parts of a solubilizer;
9-12 parts of a dispersant;
0.03-0.09 part by weight of ethylenediamine tetraacetic acid;
0.01-0.02 weight part of sodium citrate;
0.02-0.06 parts of pH value regulator;
0.02-0.04 parts by weight of an anti-interference agent; and water.
In one embodiment of the present invention, the nonionic surfactant is an isomeric alcohol ethoxylate-propylene oxide block copolymer.
In one embodiment of the present invention, the pH adjusting agent is citric acid.
In one embodiment of the present invention, the anti-interference agent is maleic acid-sodium acrylate copolymer.
The maleic acid-sodium acrylate copolymer is used as anti-interference agent for regulating different water quality and hardness.
In an embodiment of the present invention, the solubilizer is sodium gluconate.
In one embodiment of the present invention, the dispersant is selected from sodium sulfate or zinc sulfate.
In one embodiment of the present invention, the method for preparing the bio-esterase solution comprises:
(1) adding Bacillus (Bacillus sp.) WB2 into the culture solution, and continuously inducing and culturing the initial bacterial solution to obtain a Bacillus fermentation solution;
(2) centrifuging the bacillus fermentation liquor obtained in the step (1), removing supernatant, washing, transferring the bacterial suspension into Hanks liquid, heating for thermal stimulation, and cooling;
(3) crushing thallus, centrifuging, and collecting supernatant; and directly separating and concentrating the supernatant by a tangential flow ultrafiltration system to obtain the biological esterase solution.
The biological esterase solution prepared by the method has high-purity enzyme protein, and radically solves a series of difficulties brought by traditional separation, concentration and sterilization of the biological esterase solution.
In one embodiment of the present invention, the step (1) specifically comprises the following steps: adding Bacillus (Bacillus sp.) WB2 into the culture solution, controlling the initial bacterial solution inoculation proportion to be 0.05-0.12 part by weight, and continuously culturing under the conditions of stirring speed of 100-200rpm, dissolved oxygen of 10-20%, temperature of 25-35 ℃ and pH value of 6.0-7.0 until the dry weight proportion of Bacillus is 5-6% to obtain the Bacillus fermentation solution.
In one embodiment of the present invention, the step (2) specifically comprises the following steps: and (2) centrifuging the bacillus fermentation liquor obtained in the step (1), removing supernatant, washing the thallus with Hanks liquid, transferring the bacterial suspension into a proper amount of Hanks liquid, heating to 45-60 ℃ under the conditions of stirring and dissolved oxygen of 20-40%, thermally stimulating for 10-12 hours, and cooling to 20-25 ℃.
In one embodiment of the present invention, the step (3) specifically comprises the following steps: crushing the thalli for 3 times by using a high-pressure homogenizer under the pressure of 1000-1200bar, then centrifugally separating thalli fragments, and taking supernatant; separating and concentrating the supernatant with 30KD and 80KD membranes respectively by a tangential flow ultrafiltration system; concentrating to obtain the biological esterase solution.
In an embodiment of the present invention, in the step (1), the culture solution includes trehalose, sodium citrate, sodium gluconate, ammonium sulfate, zinc sulfate, and the balance of deionized water; preferably, the culture solution comprises the following components in parts by weight: 3 parts of trehalose, 1 part of sodium citrate, 0.5 part of sodium gluconate, 5 parts of ammonium sulfate, 2 parts of zinc sulfate and the balance of deionized water.
The biological esterase adopted by the biological cleaning agent capable of degrading the ink coating has the synergistic effect of the biological esterase and the heterogeneous alcohol ethoxylate-propylene oxide block copolymer of the nonionic surfactant and other organic molecular groups, so that the efficiency of the combined action of the ink decomposition and stripping can be greatly improved, the cleaning efficiency is greatly improved, and the efficiency of the cleaning agent can be increased by more than three times.
The invention also aims to provide a preparation method of the biological cleaning agent capable of degrading the ink coating, which comprises the following steps:
and (3) uniformly mixing the biological esterase solution, sophorolipid, a nonionic surfactant, a solubilizer, a dispersant, ethylene diamine tetraacetic acid, sodium citrate, citric acid, a maleic acid-sodium acrylate copolymer and water in proportion, and adjusting the pH to 7.5 by using a pH regulator to obtain the biological cleaning agent for the ink coating.
Compared with the prior art, the invention has the following beneficial effects:
(1) the biological cleaning agent is based on the composition of biological esterase and a surfactant, has a synergistic interaction effect, can greatly enhance the permeability and the emulsification effect of the cleaning agent, and achieves the aim of high-efficiency cleaning.
(2) The synergistic effect of the sophorolipid and the biological esterase in the biological cleaning agent can greatly improve the solubilizing effect of the biological printing ink coating cleaning agent, greatly improve the pollution resistance, improve the easy aging of the cleaning agent and the self-cleaning function of the solution caused by repeated use, improve the cleaning capacity of the solution, greatly increase the cleaning effect of the cleaning agent, improve the repeated use frequency and the synergistic effect of the zinc sulfate and the citric acid, ensure that the cleaning agent has better antibacterial effect and greatly improve the storage condition of the cleaning agent.
(3) In the biological cleaning agent, the sophorolipid surfactant, the biological esterase, the isomerous alcohol ethoxylate and the propylene oxide segmented copolymer can degrade, disperse and wrap macromolecular ink lipid particles to form micromolecular ink lipid particles, so that the biological esterase can be conveniently contacted with the larger surface of the micromolecular ink lipid particles, and the ink lipid molecules can be separated and degraded more quickly and completely; the isomerol ethoxylate-propylene oxide segmented copolymer, sophorolipid and biological esterase are used in a synergistic effect and mixed with sodium gluconate, so that the efficient anti-pollution performance of the cleaning agent is greatly improved, and the cleaning force is greatly enhanced.
(4) In the biological cleaning agent, the CMC and the surface tension value of the surfactant in the solution can be obviously reduced after the high-purity biological esterase degrades the macromolecular printing ink, and the dosage of the surfactant is saved.
(5) The use of the composite surfactant in the biological cleaning agent and the synergistic effect of the biological esterase enable the saponification of the printing ink grease and the hydrolysis of the macromolecule to be independent of the hydrolysis of the printing ink grease by strong alkali, and the biological cleaning agent is neutral, mild and non-corrosive.
(6) The biological cleaning agent has simple and mature process steps and is beneficial to large-scale industrial application.
(7) The main active components of the biological cleaning agent comprise a sophorolipid surfactant, a biological esterase, an isomerous alcohol ethoxylate and a propylene oxide block copolymer, and the copolymer of maleic acid and acrylic acid is used in a compatible manner, so that the copolymer of maleic acid and acrylic acid is an excellent hard water resisting agent and has excellent antifouling performance, the repeated use frequency is greatly improved, the cleaning agent has good hard water resisting capacity, and the cleaning agent is superior to common chemical cleaning agents in the market at present.
(8) The biological cleaning agent is non-toxic, is an aqueous product, is not flammable, does not contain strong alkaline substances, cannot damage metal materials, cannot damage a zinc coating and an epoxy resin coating, and can accelerate the biodegradation of the ink coating.
The bacillus WB-2 has the strain preservation number of CGMCC No.03670, is separated from soil, has spores and gram-positive bacteria, has rough bacterial colony and is dirty and white.
Detailed Description
The following detailed description of specific embodiments of the invention is provided, but it should be understood that the scope of the invention is not limited to the specific embodiments.
Throughout the specification and claims, unless explicitly stated otherwise, the word "comprise", or variations such as "comprises" or "comprising", will be understood to imply the inclusion of a stated element or component but not the exclusion of any other element or component.
Example 1
The embodiment provides a biological cleaning agent for an efficient degradable ink coating, which comprises the following components in parts by weight (total 100 parts):
0.02 part by weight of biological esterase solution;
0.06 part by weight of sophorolipid;
4 parts by weight of a nonionic surfactant isomeric alcohol ethoxylate-propylene oxide block copolymer;
6 parts by weight of solubilizer sodium gluconate;
12 parts by weight of dispersant sodium sulfate;
0.03 part by weight of ethylenediamine tetraacetic acid;
0.02 part by weight of sodium citrate;
0.02 part by weight of pH value regulator citric acid;
0.02 part by weight of an anti-interference agent maleic acid-sodium acrylate copolymer;
the balance being water.
The preparation process of the biological cleaning agent for the ink coating comprises the following steps: and (2) uniformly mixing the biological esterase solution, sophorolipid, a nonionic surfactant isomerol ethoxylate-propylene oxide segmented copolymer, a solubilizer sodium gluconate, a dispersant sodium sulfate, ethylene diamine tetraacetic acid and water in proportion, adding an anti-interference agent maleic acid-sodium acrylate copolymer, and adjusting the pH to 7.5 by using a pH regulator citric acid, namely the biological cleaning agent for the ink coating.
In this example, the preparation process of the bio-esterase solution is as follows:
(1) adding Bacillus (Bacillus sp.) WB2 into the culture solution, controlling the inoculation proportion of the initial bacterial solution to be 0.05 part by weight, and continuously culturing under the conditions of stirring speed of 100-200rpm, dissolved oxygen of 10-20%, temperature of 25-35 ℃ and pH of 6.0-7.0 until the dry weight proportion of the Bacillus is 5-6% to obtain a Bacillus fermentation solution;
(2) taking the bacillus fermentation liquor obtained in the step (1), centrifuging, removing supernatant, washing thalli with Hanks liquid, transferring bacterial suspension into a proper amount of Hanks liquid, heating to 45-60 ℃ under the conditions of stirring and 20-40% dissolved oxygen, thermally stimulating for 10-12h, and cooling to 20-25 ℃;
(3) crushing the thalli for 3 times by using a high-pressure homogenizer under the pressure of 1000-1200bar, then centrifugally separating thalli fragments, and taking supernatant; separating and concentrating the supernatant with 30KD and 80KD membranes respectively by a tangential flow ultrafiltration system; concentrating to obtain the biological esterase solution.
In this embodiment, in step (1), the culture solution includes, by weight, 3 parts of trehalose, 1 part of sodium citrate, 0.5 part of sodium gluconate, 5 parts of ammonium sulfate, 2 parts of zinc sulfate, and the balance deionized water; and sterilizing the culture solution for later use.
Example 2
The embodiment provides a biological cleaning agent for an efficient degradable ink coating, which comprises the following components in parts by weight (total 100 parts):
biological esterase solution: 0.03 part by weight;
sophorolipid: 0.06 part by weight;
nonionic surfactant isomeric alcohol ethoxylate-propylene oxide block copolymer: 6 parts by weight;
solubilizer sodium gluconate: 8 parts by weight;
sodium sulfate as a dispersant: 12 parts by weight;
ethylene diamine tetraacetic acid: 0.03 part by weight;
sodium citrate: 0.02 parts by weight;
pH value regulator citric acid: 0.02 parts by weight;
anti-interference agent maleic acid-sodium acrylate copolymer: 0.02 parts by weight;
the balance being water.
In this example, the preparation process of the bioprotein solution is as follows:
(1) adding Bacillus (Bacillus sp.) WB2 into the culture solution, controlling the initial bacterial solution inoculation proportion at 0.12 weight part, and continuously culturing under the conditions of stirring speed of 100-200rpm, dissolved oxygen of 10-20%, temperature of 25-35 ℃ and pH of 6.0-7.0 until the dry weight proportion of Bacillus is 5-6% to obtain Bacillus fermentation liquor;
(2) taking the bacillus fermentation liquor obtained in the step (1), centrifuging, removing supernatant, washing thalli with Hanks liquid, transferring bacterial suspension into a proper amount of Hanks liquid, heating to 45-60 ℃ under the conditions of stirring and 20-40% dissolved oxygen, thermally stimulating for 10-12h, and cooling to 20-25 ℃;
(3) crushing the thalli for 3 times by using a high-pressure homogenizer under the pressure of 1000-1200bar, then centrifugally separating thalli fragments, and taking supernatant; separating and concentrating the supernatant with 30KD and 80KD membranes respectively by a tangential flow ultrafiltration system; concentrating to obtain the biological esterase solution.
Wherein, in the step (1), the culture solution consists of 0.3 of trehalose, 0.1 of sodium citrate, 0.05 of sodium gluconate, 0.5 of ammonium sulfate, 0.2 of zinc sulfate and the balance of deionized water, and is used after sterilization.
In this embodiment, in step (1), the culture solution includes, by weight, 3 parts of trehalose, 1 part of sodium citrate, 0.5 part of sodium gluconate, 5 parts of ammonium sulfate, 2 parts of zinc sulfate, and the balance deionized water; and sterilizing the culture solution for later use.
Example 3
The embodiment provides a biological cleaning agent for an efficient degradable ink coating, which comprises the following components in parts by weight:
biological esterase solution: 0.06 part by weight;
sophorolipid: 0.09 parts by weight;
nonionic surfactant isomeric alcohol ethoxylate-propylene oxide block copolymer: 9 parts by weight;
solubilizer sodium gluconate: 4 parts by weight;
sodium sulfate as a dispersant: 9 parts by weight;
ethylene diamine tetraacetic acid: 0.03 part by weight;
sodium citrate: 0.02 parts by weight;
pH value regulator citric acid: 0.02 parts by weight;
anti-interference agent maleic acid-sodium acrylate copolymer: 0.02 parts by weight;
the balance being water.
In this example, the preparation process of the bio-esterase solution is as follows:
(1) adding Bacillus (Bacillus sp.) WB2 into the culture solution, controlling the inoculation proportion of the initial bacterial solution to be 0.09 weight part, and continuously culturing under the conditions of stirring speed of 100-200rpm, dissolved oxygen of 10-20%, temperature of 25-35 ℃ and pH of 6.0-7.0 until the dry weight proportion of the Bacillus is 5-6% to obtain Bacillus fermentation liquor;
(2) taking the bacillus fermentation liquor obtained in the step (1), centrifuging, removing supernatant, washing thalli with Hanks liquid, transferring bacterial suspension into a proper amount of Hanks liquid, heating to 45-60 ℃ under the conditions of stirring and 20-40% dissolved oxygen, thermally stimulating for 10-12h, and cooling to 20-25 ℃;
(3) crushing the thalli for 3 times by using a high-pressure homogenizer under the pressure of 1000-1200bar, then centrifugally separating thalli fragments, and taking supernatant; separating and concentrating the supernatant with 30KD and 80KD membranes respectively by a tangential flow ultrafiltration system; and concentrating to obtain the biological esterase solution.
In this embodiment, in step (1), the culture solution includes, by weight, 3 parts of trehalose, 1 part of sodium citrate, 0.5 part of sodium gluconate, 5 parts of ammonium sulfate, 2 parts of zinc sulfate, and the balance deionized water; and sterilizing the culture solution for later use.
Example 4
The embodiment provides a biological cleaning agent for an efficient degradable ink coating, which comprises the following components in parts by weight:
biological esterase solution: 0.03 part by weight;
sophorolipid: 0.07 part by weight;
nonionic surfactant isomeric alcohol ethoxylate-propylene oxide block copolymer: 6 parts by weight;
solubilizer sodium gluconate: 8 parts by weight;
sodium sulfate as a dispersant: 9 parts by weight;
ethylene diamine tetraacetic acid: 0.03 part by weight;
sodium citrate: 0.02 parts by weight;
pH value regulator citric acid: 0.02 parts by weight;
anti-interference agent maleic acid-sodium acrylate copolymer: 0.02 parts by weight;
the balance being water.
In this example, the preparation process of the bio-esterase solution is as follows:
(1) adding Bacillus oilreducing WB2 into the culture solution, controlling the initial bacterial solution inoculation proportion to be 0.06 weight part, and continuously culturing under the conditions of stirring speed of 100-200rpm, dissolved oxygen of 10-20%, temperature of 25-35 ℃ and pH of 6.0-7.0 until the dry weight proportion of the Bacillus is 5-6% to obtain Bacillus fermentation liquor;
(2) taking the bacillus fermentation liquor obtained in the step (1), centrifuging, removing supernatant, washing thalli with Hanks liquid, transferring bacterial suspension into a proper amount of Hanks liquid, heating to 45-60 ℃ under the conditions of stirring and 20-40% dissolved oxygen, thermally stimulating for 10-12h, and cooling to 20-25 ℃;
(3) crushing the thalli for 3 times by using a high-pressure homogenizer under the pressure of 1000-1200bar, then centrifugally separating thalli fragments, and taking supernatant; separating and concentrating the supernatant with 30KD and 80KD membranes respectively by a tangential flow ultrafiltration system; concentrating to obtain the biological esterase solution.
In this embodiment, in step (1), the culture solution includes, by weight, 3 parts of trehalose, 1 part of sodium citrate, 0.5 part of sodium gluconate, 5 parts of ammonium sulfate, 2 parts of zinc sulfate, and the balance deionized water; and sterilizing the culture solution for later use.
Example 5
The embodiment provides a biological cleaning agent for an efficient degradable ink coating, which comprises the following components in parts by weight:
biological esterase solution: 0.04 parts by weight;
sophorolipid: 0.06 part by weight;
nonionic surfactant isomeric alcohol ethoxylate-propylene oxide block copolymer: 9 parts by weight;
solubilizer sodium gluconate: 4 parts by weight;
sodium sulfate as a dispersant: 11 parts by weight;
ethylene diamine tetraacetic acid: 0.09 parts by weight;
sodium citrate: 0.01 part by weight;
pH value regulator citric acid: 0.06 part by weight;
anti-interference agent maleic acid-sodium acrylate copolymer: 0.04 parts by weight;
the balance being water.
In this example, the preparation process of the bio-esterase solution is as follows:
(1) adding Bacillus oilreducing WB2 into the culture solution, controlling the initial bacterial solution inoculation proportion to be 0.09 weight part, and continuously culturing under the conditions of stirring speed of 100-200rpm, dissolved oxygen of 10-20%, temperature of 25-35 ℃ and pH of 6.0-7.0 until the dry weight proportion of the Bacillus is 5-6% to obtain Bacillus fermentation liquor;
(2) taking the bacillus fermentation liquor obtained in the step (1), centrifuging, removing supernatant, washing thalli with Hanks liquid, transferring bacterial suspension into a proper amount of Hanks liquid, heating to 45-60 ℃ under the conditions of stirring and 20-40% dissolved oxygen, thermally stimulating for 10-12h, and cooling to 20-25 ℃;
(3) crushing the thalli for 3 times by using a high-pressure homogenizer under the pressure of 1000-1200bar, then centrifugally separating thalli fragments, and taking supernatant; separating and concentrating the supernatant with 30KD and 80KD membranes respectively by a tangential flow ultrafiltration system; concentrating to obtain the biological esterase solution.
In this embodiment, in step (1), the culture solution includes, by weight, 3 parts of trehalose, 1 part of sodium citrate, 0.5 part of sodium gluconate, 5 parts of ammonium sulfate, 2 parts of zinc sulfate, and the balance deionized water; and sterilizing the culture solution for later use.
Example 6
The embodiment provides a biological cleaning agent for an efficient degradable ink coating, which comprises the following components in parts by weight:
biological esterase solution: 0.07 part by weight;
sophorolipid: 0.08 parts by weight;
nonionic surfactant isomeric alcohol ethoxylate-propylene oxide block copolymer: 10 parts by weight;
solubilizer sodium gluconate: 3 parts by weight;
sodium sulfate as a dispersant: 9 parts by weight;
ethylene diamine tetraacetic acid: 0.04 parts by weight;
sodium citrate: 0.02 parts by weight;
pH value regulator citric acid: 0.05 part by weight;
anti-interference agent maleic acid-sodium acrylate copolymer: 0.02 parts by weight;
the balance being water.
In this example, the preparation process of the bio-esterase solution is as follows:
(1) adding Bacillus oilreducing WB2 into the culture solution, controlling the initial bacterial solution inoculation proportion to be 0.07 weight part, and continuously culturing under the conditions of stirring speed of 100-200rpm, dissolved oxygen of 10-20%, temperature of 25-35 ℃ and pH of 6.0-7.0 until the dry weight proportion of the Bacillus is 5-6% to obtain Bacillus fermentation liquor;
(2) taking the bacillus fermentation liquor obtained in the step (1), centrifuging, removing supernatant, washing thalli with Hanks liquid, transferring bacterial suspension into a proper amount of Hanks liquid, heating to 45-60 ℃ under the conditions of stirring and 20-40% dissolved oxygen, thermally stimulating for 10-12h, and cooling to 20-25 ℃;
(3) crushing the thalli for 3 times by using a high-pressure homogenizer under the pressure of 1000-1200bar, then centrifugally separating thalli fragments, and taking supernatant; separating and concentrating the supernatant with 30KD and 80KD membranes respectively by a tangential flow ultrafiltration system; concentrating to obtain the biological esterase solution.
In this embodiment, in step (1), the culture solution includes, by weight, 3 parts of trehalose, 1 part of sodium citrate, 0.5 part of sodium gluconate, 5 parts of ammonium sulfate, 2 parts of zinc sulfate, and the balance deionized water; and sterilizing the culture solution for later use.
Comparative example 1
The comparative example is a commercially available chemical cleaning agent for printing ink, and is a high-efficiency water-based cleaning agent which is made by Beijing water-based chemical company Limited and has the model number of SJ-1021.
Comparative example 2
The comparative example is a strong alkaline industrial cleaning agent (pH 12.0) which is from Beijing Bingshi technology Co., Ltd and is BL-102 normal temperature cleaning agent.
Application example 1
(1) Preparing an artificial ink coating:
the artificial ink comprises the following components in parts by weight: 30% of No. 15 hydraulic oil, 35% of industrial white vaseline and 35% of carbon black; the raw materials are uniformly mixed, uniformly coated on a metal test piece at about 105 ℃, baked for 10 minutes, cooled and accurately weighed for later use.
(2) The cleaning agents prepared in examples 1-6 and comparative examples 1-2 were used to clean the obtained metal test pieces of the artificial ink coating according to the national standard GB/T35759-2017 (metal cleaning agent), and the effects are shown in Table 1. And calculating to obtain cleaning rate data so as to check and check the cleaning effect.
Wherein η ═ (A3-a1)/(a2-a 1). Wherein, A2, A3 and A1 are the weight of the workpiece before and after the workpiece is inked and the weight of the test piece respectively.
It can be seen that the cleaning rates of the biological cleaning agent of the ink coating materials of examples 1 to 6 are higher than those of the chemical cleaning agent of comparative example 1 and the domestic commercial strong alkaline industrial cleaning agent of comparative example 2, and the best effect is 99.4%, but the cleaning rate of comparative example 1 is only 39.5%, and the cleaning rate of comparative example 2 is 49.4%, which are lower than those of the above examples. It can be seen that the bio-cleaner for the ink coating materials of examples 1 to 6 can significantly reduce the critical micelle concentration of the surfactant and the surface tension in water after adding the bio-esterase solution, thereby significantly enhancing the permeability and emulsifying ability thereof. Meanwhile, the biodegradability of waste liquid after the ink emulsification can be obviously improved. Therefore, the biological cleaning agent for the ink coating of the embodiments 1 to 6 is prepared by mixing the biological esterase solution with the surfactant, the solubilizer, the complexing agent and the like, and has the characteristics of high cleaning efficiency, mild neutrality, environmental protection, safety and the like.
Application example 2
(1) The biological esterase prepared in example 1 was diluted with deionized water to 50% and 80% by mass, and stirred and aerated, and the Dissolved Oxygen (DO) in 2 bioreactors was controlled to 2-4 mg/L. After 48 hours, another sample was taken and left to stand to measure the COD value of the supernatant, and the change of the COD value before and after the comparison was carried out, the results are shown in Table 2.
TABLE 2
| Item | Application example 1 | Application example 2 |
| Initial value | 387 | 419 |
| After 48 hours | 72 | 83 |
Various corresponding changes and modifications can be made by those skilled in the art based on the above technical solutions and concepts, and all such changes and modifications should be included in the protection scope of the present invention.
Claims (10)
1. The biological cleaning agent capable of degrading the ink coating is characterized by comprising the following components in parts by weight:
0.02-0.07 part by weight of biological esterase solution;
0.06-0.09 parts by weight of sophorolipid;
4-10 parts of nonionic surfactant;
3-8 parts of a solubilizer;
9-12 parts of a dispersant;
0.03-0.09 part by weight of ethylenediamine tetraacetic acid;
0.01-0.02 weight part of sodium citrate;
0.02-0.06 parts of pH value regulator;
0.02-0.04 parts by weight of an anti-interference agent; and
and (3) water.
2. The biological cleaning agent capable of degrading the ink paint according to claim 1, wherein the nonionic surfactant is an isomeric alcohol ethoxylate-propylene oxide block copolymer; the pH value regulator is citric acid.
3. The biological cleaning agent capable of degrading the ink paint according to claim 1, wherein the anti-interference agent is maleic acid-sodium acrylate copolymer.
4. The biological cleaning agent capable of degrading the ink coating according to claim 1, wherein the solubilizer is sodium gluconate; the dispersant is selected from sodium sulfate or zinc sulfate.
5. The biological cleaning agent capable of degrading the ink paint according to claim 1, wherein the preparation method of the biological esterase solution comprises the following steps:
(1) adding Bacillus (Bacillus sp.) WB2 into the culture solution, and carrying out continuous induction culture to obtain a Bacillus fermentation solution;
(2) centrifuging the bacillus fermentation liquor obtained in the step (1), removing supernatant, washing, transferring the bacterial suspension into Hanks liquid, heating for thermal stimulation, and cooling;
(3) crushing thallus, centrifuging, and collecting supernatant; separating and concentrating enzyme protein of the supernatant through a tangential flow ultrafiltration system, and concentrating to obtain the biological esterase solution.
6. The biological cleaning agent capable of degrading the ink paint according to claim 5, wherein the step (1) comprises the following specific steps: adding Bacillus (Bacillus sp.) WB2 into the culture solution, controlling the initial bacterial solution inoculation proportion to be 0.05-0.12 part by weight, and carrying out continuous induction culture under the conditions of stirring rotation speed of 100-200rpm, dissolved oxygen of 10-20%, temperature of 25-35 ℃ and pH value of 6.0-7.0 until the dry weight proportion of the Bacillus is 5-6% to obtain the Bacillus fermentation liquor.
7. The biological cleaning agent capable of degrading the ink paint according to claim 5, wherein the step (2) comprises the following specific steps: and (2) centrifuging the bacillus fermentation liquor obtained in the step (1), removing supernatant, washing the thallus with Hanks liquid, transferring the bacterial suspension into a proper amount of Hanks liquid, heating to 45-60 ℃ under the conditions of stirring and dissolved oxygen of 20-40%, thermally stimulating for 10-12 hours, and cooling to 20-25 ℃.
8. The biological cleaning agent capable of degrading the ink paint according to claim 5, wherein the step (3) comprises the following specific steps: crushing the thalli for 3 times by using a high-pressure homogenizer under the pressure of 1000-1200bar, then centrifugally separating thalli fragments, and taking supernatant; separating and concentrating the supernatant with 30KD and 80KD membranes respectively by a tangential flow ultrafiltration system; concentrating to obtain the biological esterase solution.
9. The biological cleaning agent for degradable ink coatings according to claim 5 or 6, characterized in that in the step (1), the culture solution comprises trehalose, sodium citrate, sodium gluconate, ammonium sulfate, zinc sulfate and the balance of deionized water; preferably, the culture solution comprises the following components in parts by weight: 3 parts of trehalose, 1 part of sodium citrate, 0.5 part of sodium gluconate, 5 parts of ammonium sulfate, 2 parts of zinc sulfate and the balance of deionized water.
10. The preparation method of the biological cleaning agent for the degradable ink coating according to claim 1, characterized by comprising the following steps:
and (3) uniformly mixing the biological esterase solution, sophorolipid, a nonionic surfactant, a solubilizer, a dispersant, ethylene diamine tetraacetic acid, sodium citrate, citric acid, a maleic acid-sodium acrylate copolymer and water in proportion, and adjusting the pH to 7.5 by using a pH regulator to obtain the biological cleaning agent for the ink coating.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111673993.5A CN114250008A (en) | 2021-12-31 | 2021-12-31 | Biological cleaning agent capable of degrading printing ink coating and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111673993.5A CN114250008A (en) | 2021-12-31 | 2021-12-31 | Biological cleaning agent capable of degrading printing ink coating and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114250008A true CN114250008A (en) | 2022-03-29 |
Family
ID=80799018
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111673993.5A Pending CN114250008A (en) | 2021-12-31 | 2021-12-31 | Biological cleaning agent capable of degrading printing ink coating and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114250008A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115505287A (en) * | 2022-10-18 | 2022-12-23 | 中国人民解放军陆军装甲兵学院 | Environment-friendly paint remover containing microbial glue and preparation method thereof |
| CN115595012A (en) * | 2022-10-18 | 2023-01-13 | 中国人民解放军陆军装甲兵学院（Cn） | Biodegradable environment-friendly paint remover and preparation method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0761263A (en) * | 1993-08-27 | 1995-03-07 | Tenryu Ind Co Ltd | Cantilever supporting device for vehicular seat |
| CN102586033A (en) * | 2011-01-13 | 2012-07-18 | 郑涵 | Biological remediation type water system oil stain cleaning agent and preparation method thereof |
| CN107974357A (en) * | 2017-10-31 | 2018-05-01 | 建德市环保科技创新创业中心有限公司 | A kind of heavy oil dirt biological cleaner and preparation method thereof |
| CN109294765A (en) * | 2018-11-14 | 2019-02-01 | 合肥华盖生物科技有限公司 | A kind of safety and environmental protection micro-organism washing agent and preparation method thereof |
| CN109576079A (en) * | 2019-01-08 | 2019-04-05 | 永发印务(东莞)有限公司 | A kind of water-based ink cleaning agent of efficient and environment-friendly type low-corrosiveness and preparation method thereof |
| CN111410864A (en) * | 2020-04-24 | 2020-07-14 | 西安理工大学 | Lipase type water-based ink cleaning agent and preparation method thereof |
-
2021
- 2021-12-31 CN CN202111673993.5A patent/CN114250008A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0761263A (en) * | 1993-08-27 | 1995-03-07 | Tenryu Ind Co Ltd | Cantilever supporting device for vehicular seat |
| CN102586033A (en) * | 2011-01-13 | 2012-07-18 | 郑涵 | Biological remediation type water system oil stain cleaning agent and preparation method thereof |
| CN107974357A (en) * | 2017-10-31 | 2018-05-01 | 建德市环保科技创新创业中心有限公司 | A kind of heavy oil dirt biological cleaner and preparation method thereof |
| CN109294765A (en) * | 2018-11-14 | 2019-02-01 | 合肥华盖生物科技有限公司 | A kind of safety and environmental protection micro-organism washing agent and preparation method thereof |
| CN109576079A (en) * | 2019-01-08 | 2019-04-05 | 永发印务(东莞)有限公司 | A kind of water-based ink cleaning agent of efficient and environment-friendly type low-corrosiveness and preparation method thereof |
| CN111410864A (en) * | 2020-04-24 | 2020-07-14 | 西安理工大学 | Lipase type water-based ink cleaning agent and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| ULRICHHOKE等: "《回收纤维与脱墨》", 28 February 2018, 中国轻工业出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115505287A (en) * | 2022-10-18 | 2022-12-23 | 中国人民解放军陆军装甲兵学院 | Environment-friendly paint remover containing microbial glue and preparation method thereof |
| CN115595012A (en) * | 2022-10-18 | 2023-01-13 | 中国人民解放军陆军装甲兵学院（Cn） | Biodegradable environment-friendly paint remover and preparation method thereof |
| CN115505287B (en) * | 2022-10-18 | 2023-10-10 | 中国人民解放军陆军装甲兵学院 | Environment-friendly paint remover containing microbial glue and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN114250008A (en) | Biological cleaning agent capable of degrading printing ink coating and preparation method thereof | |
| CN102815795B (en) | Method for processing starch wastewater as well as product thereof | |
| DE69122687T2 (en) | Cellulase, process for their preparation and their use | |
| CN105462903A (en) | Efficient sewage treatment microbial agent | |
| CN102559414A (en) | Biological degreasing agent | |
| US20150267151A1 (en) | Protein-enhanced surfactants for enzyme activation | |
| CN103805526A (en) | Strain producing surface active agent resistance lipase and surface active agent resistance lipase preparation method | |
| CN107974357A (en) | A kind of heavy oil dirt biological cleaner and preparation method thereof | |
| Ire et al. | Production, purification and characterization of polygalacturonase from Aspergillus niger in solid state and submerged fermentation using banana peels | |
| CN106119156A (en) | A kind of preparation method of the microorganism formulation cleaned for greasy dirt | |
| CN110922975A (en) | Preparation method and application of microbial straw degradation microbial inoculum | |
| CN110628688A (en) | Bacillus subtilis for producing gamma-polyglutamic acid and application thereof | |
| CN107746822A (en) | Salt affected soil repairs microbial bacterial agent and preparation method thereof | |
| Prasertsan et al. | Treatment of palm oil mill effluent by thermotolerant polymer-producing fungi | |
| CN102219286A (en) | Treatment method of wastewater generated during citric acid preparation and preparation method of citric acid | |
| CN110143732B (en) | Method for treating waste water generated in vegetable oil byproduct processing | |
| CN107201382B (en) | A kind of detergent and its preparation method and application | |
| CN114230690B (en) | Preparation method of low-heat-generation high-performance natural rubber | |
| CN116333828A (en) | Biological cleaning agent capable of degrading heavy oil dirt and preparation method thereof | |
| CN115975857A (en) | Broad-spectrum culture medium suitable for degrading waste feathers and application thereof | |
| CN109679865A (en) | A kind of microorganism blender and its preparation preparation and application | |
| CN104556405B (en) | A kind of Biological compound flocculant containing ferric ion and application thereof | |
| CN114540253A (en) | Application of bacillus pumilus P6 keratinase in detergent and application method | |
| CN110643668B (en) | Method for extracting chitin from shrimp shells by utilizing microbial fermentation | |
| CN105800798B (en) | A kind of microbial bacterial agent and its application method handling printing and dyeing industrial waste water |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20220329 |
|
| RJ01 | Rejection of invention patent application after publication |