CN114246993A - Composite coating with adjustable active ingredient release performance and preparation method thereof - Google Patents

Composite coating with adjustable active ingredient release performance and preparation method thereof Download PDF

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Publication number
CN114246993A
CN114246993A CN202111517486.2A CN202111517486A CN114246993A CN 114246993 A CN114246993 A CN 114246993A CN 202111517486 A CN202111517486 A CN 202111517486A CN 114246993 A CN114246993 A CN 114246993A
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active ingredient
composite coating
release performance
ingredient release
preparing
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CN114246993B (en
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张波涛
潘永红
叶磊
陈秀勇
黄晶
吴双杰
周平
李华
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Ningbo Institute of Material Technology and Engineering of CAS
Cixi Institute of Biomedical Engineering CIBE of CAS
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Ningbo Institute of Material Technology and Engineering of CAS
Cixi Institute of Biomedical Engineering CIBE of CAS
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
    • A61L31/14—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L31/16—Biologically active materials, e.g. therapeutic substances
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
    • A61L31/08—Materials for coatings
    • A61L31/10—Macromolecular materials
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
    • B05D—PROCESSES FOR APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
    • B05D1/00—Processes for applying liquids or other fluent materials
    • B05D1/02—Processes for applying liquids or other fluent materials performed by spraying
    • B05D1/08—Flame spraying
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
    • B05D—PROCESSES FOR APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
    • B05D3/00—Pretreatment of surfaces to which liquids or other fluent materials are to be applied; After-treatment of applied coatings, e.g. intermediate treating of an applied coating preparatory to subsequent applications of liquids or other fluent materials
    • B05D3/002—Pretreatement
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
    • B05D—PROCESSES FOR APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
    • B05D7/00—Processes, other than flocking, specially adapted for applying liquids or other fluent materials to particular surfaces or for applying particular liquids or other fluent materials
    • B05D7/24—Processes, other than flocking, specially adapted for applying liquids or other fluent materials to particular surfaces or for applying particular liquids or other fluent materials for applying particular liquids or other fluent materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
    • A61L2300/406—Antibiotics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
    • A61L2300/606—Coatings
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Engineering & Computer Science (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Surgery (AREA)
  • Vascular Medicine (AREA)
  • Epidemiology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Materials For Medical Uses (AREA)

Abstract

The invention discloses a preparation method of a composite coating with adjustable active ingredient release performance, which comprises the following steps: (1) preparing a mixed solution from at least two polymers and at least one active ingredient; (2) cleaning and roughening the surface of the matrix; (3) spraying the mixed solution obtained in the step (1) on the surface of the treated substrate by using a flame spraying process to prepare the composite coating with adjustable active ingredient release performance; the active ingredients comprise antibacterial agents, anticoagulants, antiplatelet drugs, angiogenesis inhibitors, hemostatic agents, antitumor agents, immunosuppressants and osteoinductive agents. The method has good repeatability, high safety and small influence on active ingredients, solves the problem that solvent systems among different macromolecules are difficult to construct, can combine the excellent characteristics of the different macromolecules, and the prepared composite coating with the adjustable release performance of the active ingredients can realize the release of the active ingredients at different rates.

Description

Composite coating with adjustable active ingredient release performance and preparation method thereof
Technical Field
The invention relates to the technical field of composite coatings, in particular to a composite coating with adjustable active ingredient release performance and a preparation method thereof.
Background
The conventional medical materials have limited applications due to lack of antibacterial property, biological activity and osteoinductivity, so that surface functional modification is usually required. The polymer material has rich varieties, various mechanical properties, stable physical properties and different biocompatibility, so the polymer material is widely used for preparing the surface functionalized modified coating. However, because the slow release properties of different polymer coatings are very different and the solubility in different solvents is different, the conventional blending and compounding method after dissolution is severely restricted, and the release properties of a wide variety of polymer materials are difficult to be subjected to wide compound regulation and control. The flame spraying technology takes the polymer solution or suspension as the raw material to prepare the coating, is very suitable for preparing composite coatings of different polymer materials with larger performance difference, and has wide application prospect.
Chinese patent publication No. CN107286793A discloses a method for preparing a medical coating, comprising: uniformly mixing a reactant, a hydrophilic polymer and a proper amount of solvent according to a ratio to obtain a coating solution, and coating the coating solution on the surface of an object to be coated; and (4) drying by ultraviolet radiation, and forming a hydrophilic coating after the surface of the coated object is fully dried. The method is simple and easy to operate, the prepared medical coating has good bonding firmness with medical instruments and good lubricating effect, but the coating prepared by the coating solution coating method is easy to cause the problem of uneven coating.
Chinese patent publication No. CN110665070B discloses a method for preparing a medical coating with a hydrophilic lubricating surface, comprising: uniformly coating micelle coating liquid on the outer wall of the cleaned medical product by a dipping coating process, drying in vacuum to form a coating, and obtaining a hydrophilic lubricating medical coating on the surface of the medical product, wherein the micelle coating liquid contains poloxamer, hyaluronic acid or sodium salt thereof and a preservative. Due to the fact that the poloxamer has a hydrophobic polypropylene oxide chain segment and a hydrophilic polyethylene oxide chain segment, the coating is well combined with the surface of a medical appliance.
The Chinese patent document with the publication number of CN108815552B discloses a preparation method of a biomedical coating material with controllable loading and long-acting slow release of multiple drugs, which comprises the following steps: polishing, cleaning and drying a substrate material, soaking the substrate material in a dopamine solution, taking catechol-modified polyamino biomacromolecule, electronegative macromolecular solution and micelle loaded with various medicines as three components of layer-by-layer self-assembly, and preparing the coating modified material on the dopamine-treated substrate by using the three components through a layer-by-layer self-assembly method. The invention can realize the sustained release of a plurality of medicaments in a large time sequence, but the preparation method of the coating is complex.
Disclosure of Invention
The invention provides a preparation method of a composite coating with adjustable active ingredient release performance, which has the advantages of good repeatability, high safety and small influence on active ingredients, can be combined with the excellent characteristics of different macromolecules, solves the problem that solvent systems among different macromolecules are difficult to construct, and can realize the release of the active ingredients at different rates.
The technical scheme is as follows:
a preparation method of a composite coating with adjustable active ingredient release performance comprises the following steps:
(1) preparing a mixed solution from at least two polymers and at least one active ingredient;
(2) cleaning and roughening the surface of the matrix;
(3) spraying the mixed solution obtained in the step (1) on the surface of the treated substrate by using a flame spraying process to prepare the composite coating with adjustable active ingredient release performance;
the active ingredients comprise antibacterial agents, anticoagulants, antiplatelet drugs, angiogenesis inhibitors, hemostatic agents, antitumor agents, immunosuppressants and osteoinductive agents;
in the step (1), the solid content of the mixed solution is 3-50 g/100mL, and the mass ratio of the high polymer to the active ingredient is 2-1000: 1.
The adjustable release performance of the active ingredients refers to the regulation and control of the release performance of the composite coating by compounding different macromolecules or changing the compounding ratio.
In the step (1), the mixed solution is a polymer-active ingredient mixed solution or a polymer-active ingredient mixed suspension.
The polymer has excellent spraying characteristic, and comprises perfluoropropyl perfluorovinyl ether-polytetrafluoroethylene copolymer, ethylene-tetrafluoroethylene copolymer, fluorinated ethylene propylene copolymer, ethylene-fluorinated ethylene propylene copolymer, polyethylene, polypropylene, polyvinyl chloride, polyacrylate, silicone rubber, polyimide, polyether ether ketone, polyurethane, polylactic acid, polyglycolic acid, polylactic acid-glycolic acid copolymer, polycaprolactone, polyvinyl alcohol, polybutylene succinate or polyhydroxyalkanoate.
Preferably, the polymer particle size is 15-300 μm.
Preferably, the polymer is perfluoropropyl perfluorovinyl ether-polytetrafluoroethylene copolymer, ethylene-tetrafluoroethylene copolymer, polylactic acid or polycaprolactone.
The antibacterial agent includes but is not limited to copper and its compounds, silver and its compounds, povidone iodine, triclosan, chlorhexidine gluconate, chlorhexidine acetate, quaternary ammonium salts, gentamicin sulfate, gentamicin hydrochloride, amikacin sulfate, amikacin hydrochloride, tobramycin sulfate, tobramycin hydrochloride, clindamycin hydrochloride, lincosamide hydrochloride, moxifloxacin, ciprofloxacin, teicoplanin, vancomycin, ramoplanin, metronidazole, tinidazole or amitrazole.
The anticoagulant includes but is not limited to heparin, hirudin and its derivatives, argatroban, sodium citrate.
The antiplatelet drug includes but is not limited to sarpogrelate hydrochloride.
The angiogenesis inhibitor includes but is not limited to fumagillin, fumagillol derivatives or neogenesis inhibitory steroids.
Such hemostatic agents include, but are not limited to, thrombin, thromboplastin, acetyl menadione, menadione sodium bisulfite, tranexamic acid, epsilon-aminocaproic acid, adrenochrome monoaminoguanidine methyl sulfonate, or carbazochrome sodium sulfonate.
The antineoplastic agent comprises, but is not limited to, methotrexate, actinomycin D, mitomycin C, bleomycin hydrochloride, daunomycin hydrochloride, vinblastine sulfosulfate, aldehydic vinblastine sulfate, adriamycin, neocarzinostatin, fluorouracil, cytarabine, coriolus versicolor polysaccharide K, a streptococcus sanguis preparation, lentinan, betadine, levamisole, carbamyl cyanopyridine, glycyrrhizin, cisplatin or paclitaxel.
The immunosuppressant includes but is not limited to rapamycin, cyclosporine, tacrolimus, methotrexate, azathioprine, cyclophosphamide, dexamethasone or mizoribine.
The osteoinductive agent includes, but is not limited to, bone morphogenetic protein or fibroblast growth factor.
The solvent of the mixed solution comprises: acetone, tetrahydrofuran, dimethylformamide, ethanol or an aqueous solution of ethanol.
The substrate comprises metal, alloy, glass or plastic.
Preferably, in the step (2), the surface of the cleaned substrate is roughened by a sand blasting process, and the parameters of the sand blasting process are as follows: the air pressure is 0.5-1.0 MPa, the sand blasting time is 10 s-1 min, and the mesh number of sand pills for sand blasting is 30-200 meshes.
Preferably, in the step (3), the flame spraying conditions are as follows: the combustion-supporting gas is O2Pressure of 0.1-1 MPa and flow of 1-10 Nm3H; the fuel gas is C2H2Pressure 0.1-0.5 MPa, flow 1-5 Nm3H; the auxiliary gas is compressed air, and the pressure is 0.3-5.0 MPa; the spraying feeding speed is 10-100 g/min, and the spraying distance is 150-400 mm.
The invention also provides the composite coating with adjustable active ingredient release performance, which is prepared by the preparation method of the composite coating with adjustable active ingredient release performance.
Compared with the prior art, the invention has the beneficial effects that:
(1) the invention adopts the flame spraying technology to prepare the functional coating by compounding different macromolecules and active ingredients, overcomes the problems of larger solubility difference between different macromolecules and active ingredients and difficult construction of a solvent system compared with the electrostatic spinning technology and the tape casting technology, and ensures the safety of experimental operation.
(2) The method can be combined with the excellent characteristics of different macromolecules, such as elasticity, hardness, insulativity, conductivity and the like, and prepare the multifunctional composite coating with adjustable active ingredient release performance, which is suitable for different application scenes.
(3) The preparation method is simple, good in repeatability, small in influence on the active ingredients and suitable for large-scale application, and the prepared coating is a uniform and continuous coating and can effectively realize release of the active ingredients at different rates.
Detailed Description
The invention will be further elucidated with reference to the following examples. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention.
Example 1
(1) The method comprises the steps of adding 15g of polylactic acid (with the particle size of 30-100 mu m) and 5g of polycaprolactone (with the particle size of 15-55 mu m) into 200mL of 50% ethanol aqueous solution serving as a solvent to obtain a mixed polymer suspension, and adding 0.5g of gentamicin sulfate to prepare a mixed solution.
(2) Cleaning the surface of a substrate: the substrate is a stainless steel sheet, and is cleaned by acetone, alcohol and deionized water in sequence and then dried by nitrogen for later use; roughening the surface of the matrix: adopting a sand blasting process to carry out roughening treatment on the surface of the cleaned matrix so as to increase the surface roughness of the matrix and improve the bonding strength of the coating, wherein the parameters of the sand blasting process are as follows: the air pressure is 0.8MPa, the sand blasting time is 20s, and the mesh number of sand pills for sand blasting is 50 meshes;
(3) spraying the mixed liquid obtained in the step (1) on the surface of the treated substrate by using a flame spraying process, wherein the flame spraying conditions are as follows: the combustion-supporting gas is O2Pressure 0.5MPa, flow 2Nm3H; the fuel gas is C2H2Pressure 0.1MPa, flow 2Nm3H; the auxiliary gas is compressed air, the pressure is 0.8MPa, the feeding speed is 20g/min, and the spraying distance is 200mm, so that the composite coating with adjustable active ingredient release performance is prepared.
Example 2
(1) 200mL of 50% ethanol aqueous solution is used as a solvent, 5g of polylactic acid (with the particle size of 30-100 mu m) and 15g of polycaprolactone (with the particle size of 15-55 mu m) are added to obtain a mixed polymer suspension, and 0.5g of gentamicin sulfate is added to prepare a mixed solution.
(2) Cleaning the surface of a substrate: selecting glass as a substrate, sequentially cleaning the glass with acetone, alcohol and deionized water, and drying the glass with nitrogen for later use; roughening the surface of the matrix: adopting a sand blasting process to carry out roughening treatment on the surface of the cleaned matrix so as to increase the surface roughness of the matrix and improve the bonding strength of the coating, wherein the parameters of the sand blasting process are as follows: the air pressure is 0.8MPa, the sand blasting time is 20s, and the mesh number of sand pills for sand blasting is 50 meshes;
(3) spraying the mixed liquid obtained in the step (1) on the surface of the treated substrate by using a flame spraying process, wherein the flame spraying conditions are as follows: the combustion-supporting gas is O2Pressure 0.5MPa, flow 2Nm3H; the fuel gas is C2H2Pressure 0.1MPa, flow 2Nm3H; the auxiliary gas is compressed air, the pressure is 0.8MPa, the feeding speed is 20g/min, and the spraying distance is 200mm, so that the composite coating with adjustable active ingredient release performance is prepared.
Example 3
(1) 200mL of 50% ethanol aqueous solution is used as a solvent, 10g of polylactic acid (with the particle size of 30-100 mu m) and 10g of perfluoropropyl perfluorovinyl ether-polytetrafluoroethylene copolymer (with the particle size of 30-100 mu m) are added to obtain a mixed polymer suspension, and 0.5g of gentamicin sulfate is added to prepare a mixed solution.
(2) Cleaning the surface of a substrate: the substrate is a stainless steel sheet, and is cleaned by acetone, alcohol and deionized water in sequence and then dried by nitrogen for later use; roughening the surface of the matrix: adopting a sand blasting process to carry out roughening treatment on the surface of the cleaned matrix so as to increase the surface roughness of the matrix and improve the bonding strength of the coating, wherein the parameters of the sand blasting process are as follows: the air pressure is 0.8MPa, the sand blasting time is 20s, and the mesh number of sand pills for sand blasting is 50 meshes;
(3) spraying the mixed liquid obtained in the step (1) on the surface of the treated substrate by using a flame spraying process, wherein the flame spraying conditions are as follows: the combustion-supporting gas is O2Pressure 0.5MPa, flow 2Nm3H; the fuel gas is C2H2Pressure 0.1MPa, flow 2Nm3H; the auxiliary gas is compressed air, the pressure is 0.8MPa, the feeding speed is 15g/min, and the spraying distance is 150mm, so that the composite coating with the adjustable release performance of the active ingredients is prepared.
Example 4
(1) The method comprises the steps of adding 5g of polylactic acid (with the particle size of 30-100 mu m) and 15g of ethylene-tetrafluoroethylene copolymer (with the particle size of 30-100 mu m) into 200mL of 70% ethanol aqueous solution serving as a solvent to obtain a mixed polymer suspension, and adding 0.5g of chlorhexidine gluconate to prepare a mixed solution.
(2) Cleaning the surface of a substrate: the substrate is a stainless steel sheet, and is cleaned by acetone, alcohol and deionized water in sequence and then dried by nitrogen for later use; roughening the surface of the matrix: adopting a sand blasting process to carry out roughening treatment on the surface of the cleaned matrix so as to increase the surface roughness of the matrix and improve the bonding strength of the coating, wherein the parameters of the sand blasting process are as follows: the air pressure is 0.8MPa, the sand blasting time is 30s, and the mesh number of sand pills for sand blasting is 100 meshes;
(3) spraying the mixed liquid obtained in the step (1) on the surface of the treated substrate by using a flame spraying process, wherein the flame spraying conditions are as follows: the combustion-supporting gas is O2Pressure 0.5MPa, flow 2Nm3H; the fuel gas is C2H2Pressure 0.1MPa, flow 2Nm3H; the auxiliary gas is compressed air, the pressure is 0.8MPa, the feeding speed is 15g/min, and the spraying distance is 150mm, so that the composite coating with the adjustable release performance of the active ingredients is prepared.
Example 5
(1) The method comprises the steps of adding 15g of polylactic acid (with the particle size of 30-100 mu m) and 5g of ethylene-tetrafluoroethylene copolymer (with the particle size of 30-100 mu m) into 200mL of 70% ethanol aqueous solution serving as a solvent to obtain a mixed polymer suspension, and adding 0.5g of chlorhexidine gluconate to prepare a mixed solution.
(2) Cleaning the surface of a substrate: the substrate is a stainless steel sheet, and is cleaned by acetone, alcohol and deionized water in sequence and then dried by nitrogen for later use; roughening the surface of the matrix: adopting a sand blasting process to carry out roughening treatment on the surface of the cleaned matrix so as to increase the surface roughness of the matrix and improve the bonding strength of the coating, wherein the parameters of the sand blasting process are as follows: the air pressure is 0.8MPa, the sand blasting time is 30s, and the mesh number of sand pills for sand blasting is 100 meshes;
(3) spraying the mixed liquid obtained in the step (1) on the surface of the treated substrate by using a flame spraying process, wherein the flame spraying conditions are as follows: the combustion-supporting gas is O2Pressure 0.5MPa, flow 2Nm3H; the fuel gas is C2H2Pressure 0.1MPa, flow 2Nm3H; the auxiliary gas is compressed air, the pressure is 0.8MPa, the feeding speed is 15g/min, and the spraying distance is 150mm, so that the composite coating with the adjustable release performance of the active ingredients is prepared.
Sample analysis
The coating samples (2cm × 2cm) of examples 1 to 5 were soaked in 5mL of PBS solution after the side and bottom surfaces were closed with hot melt adhesive, and the amount of active ingredient released after 4 hours was measured, and the results are shown in table 1:
TABLE 1 active ingredient Release Performance of composite coatings in examples 1-5
Sample (I) Release concentration in soak solution (mg/L)
Example 1 15.6
Example 2 4.2
Example 3 9.3
Example 4 2.8
Example 5 12.5
As can be seen from the data in Table 1, the release performance of the active ingredient of the prepared composite coating can be effectively regulated and controlled by compounding different macromolecules or changing the compounding ratio.
The embodiments described above are intended to illustrate the technical solutions of the present invention in detail, and it should be understood that the above-mentioned embodiments are only specific embodiments of the present invention, and are not intended to limit the present invention, and any modification, supplement or similar substitution made within the scope of the principles of the present invention should be included in the protection scope of the present invention.

Claims (9)

1. The preparation method of the composite coating with adjustable active ingredient release performance is characterized by comprising the following steps:
(1) preparing a mixed solution from at least two polymers and at least one active ingredient;
(2) cleaning and roughening the surface of the matrix;
(3) spraying the mixed solution obtained in the step (1) on the surface of the treated substrate by using a flame spraying process to prepare the composite coating with adjustable active ingredient release performance;
the active ingredients comprise antibacterial agents, anticoagulants, antiplatelet drugs, angiogenesis inhibitors, hemostatic agents, antitumor agents, immunosuppressants and osteoinductive agents;
in the step (1), the solid content of the mixed solution is 3-50 g/100mL, and the mass ratio of the high polymer to the active ingredient is 2-1000: 1.
2. The method for preparing a composite coating with adjustable active ingredient release performance according to claim 1, wherein the polymer comprises perfluoropropyl perfluorovinyl ether-polytetrafluoroethylene copolymer, ethylene-tetrafluoroethylene copolymer, fluorinated ethylene propylene copolymer, ethylene-fluorinated ethylene propylene copolymer, polyethylene, polypropylene, polyvinyl chloride, polyacrylate, silicone rubber, polyimide, polyether ether ketone, polyurethane, polylactic acid, polyglycolic acid, polylactic acid-glycolic acid copolymer, polycaprolactone, polyvinyl alcohol, polybutylene succinate, or polyhydroxyalkanoate.
3. The method for preparing the composite coating with the adjustable active ingredient release performance according to claim 1, wherein the particle size of the polymer is 15-300 μm.
4. The method for preparing the composite coating with adjustable active ingredient release performance according to claim 1, wherein the polymer is perfluoropropyl perfluorovinyl ether-polytetrafluoroethylene copolymer, ethylene-tetrafluoroethylene copolymer, polylactic acid or polycaprolactone.
5. The method for preparing a composite coating with adjustable active ingredient release performance according to claim 1, wherein the solvent of the mixed solution comprises: acetone, tetrahydrofuran, dimethylformamide, ethanol or an aqueous solution of ethanol.
6. The method for preparing a composite coating with adjustable active ingredient release property according to claim 1, wherein the substrate comprises metal, alloy, glass or plastic.
7. The method for preparing the composite coating with adjustable active ingredient release performance according to claim 1, wherein in the step (2), the surface of the cleaned substrate is roughened by a sand blasting process, and the parameters of the sand blasting process are as follows: the air pressure is 0.5-1.0 MPa, the sand blasting time is 10 s-1 min, and the mesh number of sand pills for sand blasting is 30-200 meshes.
8. The method for preparing the composite coating with adjustable active ingredient release performance according to claim 1, wherein in the step (3), the flame spraying conditions are as follows: the combustion-supporting gas is O2Pressure of 0.1-1 MPa and flow of 1-10 Nm3H; the fuel gas is C2H2Pressure 0.1-0.5 MPa, flow 1-5 Nm3H; the auxiliary gas is compressed air, and the pressure is 0.3-5.0 MPa; the spraying feeding speed is 10-100 g/min, and the spraying distance is 150-400 mm.
9. The composite coating with adjustable active ingredient release performance prepared by the preparation method of the composite coating with adjustable active ingredient release performance according to any one of claims 1 to 8.
CN202111517486.2A 2021-12-09 2021-12-09 Composite coating with adjustable active ingredient release performance and preparation method thereof Active CN114246993B (en)

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Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20040070875A (en) * 2003-02-05 2004-08-11 학교법인 인하학원 Method for coating bioactive glasses on alumina plate
CN1791437A (en) * 2003-05-16 2006-06-21 布卢薄膜有限责任公司 Medical implants comprising biocompatible coatings
CN101214396A (en) * 2008-01-03 2008-07-09 乐普（北京）医疗器械股份有限公司 Controlled degradation magnesium alloy coating bracket and preparation thereof
WO2010130109A1 (en) * 2009-05-12 2010-11-18 四川大学 Method for preparing porous hydroxyapatite coatings by suspension plasma spraying
US20110300188A1 (en) * 2010-06-02 2011-12-08 Shimp Lawrence A Glassy calcium phosphate particulates, coatings and related bone graft materials
CN105838239A (en) * 2016-04-13 2016-08-10 中国科学院宁波材料技术与工程研究所 Polyimide composite coating and preparation method thereof and application thereof
CN106139259A (en) * 2016-08-17 2016-11-23 林春梅 One has drug slow release function blood vessel bracket coating and preparation method thereof
CN106267378A (en) * 2016-10-10 2017-01-04 同济大学 A kind of preparation method of the metal rack of controlled degradation
CN109136814A (en) * 2018-08-20 2019-01-04 陕西鑫威泰生物科技有限公司 A kind of preparation method customizing skull board for pillow polyether-ether-ketone composite coating
CN109457207A (en) * 2018-11-22 2019-03-12 九江学院 A kind of preparation method of hydroxylapatite-magnesium composite coating
CN111658798A (en) * 2020-06-15 2020-09-15 安徽静斯德科技有限公司 Mask disinfection and sterilization method based on plasma
EP3834853A1 (en) * 2019-12-13 2021-06-16 EMPA Eidgenössische Materialprüfungs- und Forschungsanstalt Coatings, coated implants and manufacturing methods therefore

Patent Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20040070875A (en) * 2003-02-05 2004-08-11 학교법인 인하학원 Method for coating bioactive glasses on alumina plate
CN1791437A (en) * 2003-05-16 2006-06-21 布卢薄膜有限责任公司 Medical implants comprising biocompatible coatings
CN101214396A (en) * 2008-01-03 2008-07-09 乐普（北京）医疗器械股份有限公司 Controlled degradation magnesium alloy coating bracket and preparation thereof
WO2010130109A1 (en) * 2009-05-12 2010-11-18 四川大学 Method for preparing porous hydroxyapatite coatings by suspension plasma spraying
US20110300188A1 (en) * 2010-06-02 2011-12-08 Shimp Lawrence A Glassy calcium phosphate particulates, coatings and related bone graft materials
CN105838239A (en) * 2016-04-13 2016-08-10 中国科学院宁波材料技术与工程研究所 Polyimide composite coating and preparation method thereof and application thereof
CN106139259A (en) * 2016-08-17 2016-11-23 林春梅 One has drug slow release function blood vessel bracket coating and preparation method thereof
CN106267378A (en) * 2016-10-10 2017-01-04 同济大学 A kind of preparation method of the metal rack of controlled degradation
CN109136814A (en) * 2018-08-20 2019-01-04 陕西鑫威泰生物科技有限公司 A kind of preparation method customizing skull board for pillow polyether-ether-ketone composite coating
CN109457207A (en) * 2018-11-22 2019-03-12 九江学院 A kind of preparation method of hydroxylapatite-magnesium composite coating
EP3834853A1 (en) * 2019-12-13 2021-06-16 EMPA Eidgenössische Materialprüfungs- und Forschungsanstalt Coatings, coated implants and manufacturing methods therefore
CN111658798A (en) * 2020-06-15 2020-09-15 安徽静斯德科技有限公司 Mask disinfection and sterilization method based on plasma

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
A.CHEBBI等: "《Thermal Spraying of Bioactive Polymer Coatings for Orthopaedic Applications》", 《JOURNAL OF THERMAL SPRAY TECHNOLOGY》, vol. 21, no. 3, pages 719 - 730 *
YONGHONG PAN 等: "《Facile suspension flame spray fabrication of polylactic acid-povidone-iodine coatings for antimicrobial applications》", 《MATERIALS LETTERS》, vol. 341, pages 134293 *

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