CN114246652B

CN114246652B - Oocyte retrieval system

Info

Publication number: CN114246652B
Application number: CN202111257945.8A
Authority: CN
Inventors: 毕胜成; 孟荣华; 王双; 毕方成; 陈春晓
Original assignee: Taipingyang Kangtai Scientific Apparatus Jinan Co ltd
Current assignee: Taipingyang Kangtai Scientific Apparatus Jinan Co ltd
Priority date: 2021-10-27
Filing date: 2021-10-27
Publication date: 2024-01-26
Anticipated expiration: 2041-10-27
Also published as: CN114246652A

Abstract

The invention discloses an oocyte retrieval system, which comprises a negative pressure suction tube, a puncture needle holding handle and an oocyte collection pipeline, wherein the puncture needle, the puncture needle holding handle and the oocyte collection pipeline are sequentially connected; one end of the oocyte collecting pipeline and one end of the negative pressure suction pipe are fixed in the sealing plug, the collecting pipeline penetrates out of the sealing plug, and the sterile collecting test pipe is clamped on the sealing plug for collecting oocytes; the tail end of the oocyte collecting pipeline is provided with an oocyte releasing cavity communicated with the oocyte collecting cavity; an egg taking channel and a flushing channel are arranged in the inner cavity of the puncture needle along the length direction; the puncture needle holding handle is connected with a push injection device for rinsing the follicle, so as to rinse the residual oocyte. The sealing plug is provided with a convex and inclined opening type oocyte collecting cavity, so that an ovum can drop downwards along the pipe wall, and the impact force of the ovum when entering liquid is reduced. The sealing plug is also designed with an ovum release cavity, so that the ovum forms a larger droplet ball, the ovum is protected in the falling process, and the ovum is prevented from staying in the pipeline.

Description

Oocyte retrieval system

Technical Field

The invention relates to the technical field of egg taking surgical instruments, in particular to an oocyte retrieval system.

Background

The incidence of infertility caused by the postponement of pregnancy plans of modern people is in a trend of increasing year by year due to various reasons, the advanced age is one of the reasons for infertility, in addition, the infertility is also influenced by factors such as environment, self pressure, drug abuse and the like, the incidence is also in a trend of increasing year by year, and the infertility patients treated by means of auxiliary reproductive technologies, in particular in vitro fertilization, are more and more. Currently, human ART mainly includes intrauterine insemination, in vitro fertilization-embryo transfer, artificial insemination, in vitro maturation of ova, single sperm injection of egg cytoplasm, ovum, sperm, embryo freeze thawing technique, genetic diagnosis before transfer, and the like. The treatment purpose of the patient is to obtain clinical gestation and healthy offspring, and the aim is to obtain high-quality ovum, improve fertilization rate and finally obtain more high-quality embryo, but the quality of ovum and embryo can be affected in multiple links such as clinical medication, ovum taking operation, embryo culture-transplantation and the like. In vitro fertilization-embryo transfer (IVF-ET) cycles are usually performed with artificial superovulation, the purpose of which is to stimulate the synchronous development of a plurality of primordial follicles to obtain a plurality of high-quality ova, so as to obtain high-quality embryos for selection and transfer, and finally improve the clinical pregnancy rate, however, the process of combining sperm and ova to form fertilized eggs and developing into embryos is very complex, and any link can affect the final result.

The egg taking operation is the first step of in-vitro operation in the treatment of in-vitro fertilization-embryo transplantation technology, equipment used in egg taking consists of an egg taking needle, a negative pressure aspirator, a negative pressure pump connecting pipe between the egg taking needle and the negative pressure aspirator, and a disposable sterile test tube, and the stability of a channel and negative pressure of a pipeline is kept during operation, that is, the negative pressure aspirator is needed to assist in egg taking work during clinical egg taking, and the stability of the negative pressure aspirator plays a key role in success and failure. The egg taking operation is to suck mature oocytes from ovarian tissues through vagina under the guidance of B ultrasonic, and is an intermediate link from the fact that the oocytes leave a human body to the culture of a laboratory incubator, and because the operation time is long, the oocytes are easily influenced by various factors, the physiological functions of the oocytes can be possibly changed, and the egg taking process is influenced by the proficiency of operators and the stability of the whole egg taking channel.

As shown in fig. 1, the conventional ovum extractor generally comprises a puncture needle, a collection tube 5', a negative pressure suction tube 6' and a sealing plug 7', wherein an ovum directly enters into a test tube through the collection tube 5', which causes unavoidable damage in the ovum collection process, and in addition, after sucking out most of the oocytes, residual oocytes attached to the ovary tissue are difficult to suck out, which affects the collection rate of the oocytes. Therefore, how to reduce the impact force of the ovum during the collection process and how to suck out the residual oocyte smoothly becomes a problem to be solved.

Disclosure of Invention

The invention aims to provide an oocyte retrieval system, which can enable an ovum to drop downwards along the tube wall in an ovum taking operation, reduce the impact force of the ovum when the ovum drops into liquid, reduce the deformation damage of the ovum, and can flush and smoothly suck out residual oocytes.

In order to achieve the above object, the present invention provides an oocyte retrieval system, including a negative pressure suction tube, and a puncture needle, a puncture needle holding handle and an oocyte collection pipeline which are sequentially connected; the end parts of the oocyte collecting pipeline and the negative pressure suction pipe are fixed inside the sealing plug, the oocyte collecting pipeline penetrates out of the sealing plug, and the sterile collecting test pipe is clamped on the sealing plug and is used for collecting the sucked oocyte; the end of the oocyte collecting pipeline is provided with an oocyte releasing cavity inside the sealing plug, and the oocyte releasing cavity is communicated with the oocyte collecting cavity; an egg taking channel and a flushing channel are arranged in parallel in the length direction of the inner cavity of the puncture needle; the puncture needle holding handle of the oocyte retrieval system is communicated with a flushing pipe and a flushing pipe connecting seat positioned at one end of the flushing pipe, and the flushing pipe connecting seat is communicated with a push injection device for follicular flushing so as to push injection flushing liquid into the ovary through a flushing channel of the puncture needle to flush residual oocytes and suck out the residual oocytes.

According to the invention, the oocyte collecting cavity extends towards the inside of the sterile collecting test tube and has a convex structure relative to the small-diameter bottom plane of the sealing plug; the part of the oocyte collecting cavity, which faces the outer side and is close to the tube wall of the sterile collecting tube, is provided with an inclined opening structure so as to protect the ovum from falling downwards along the tube wall of the sterile collecting tube connected with the oocyte collecting cavity.

Preferably, the inclination of the inclined port structure is 20-45 ° with respect to the axial direction of the sealing plug, more preferably the inclination angle is 30-40 °, for example 15 °,25 °,35 °,45 °,55 °, or any point value in the range of values formed by any two points.

Preferably, the junction of the oocyte collection cavity and the oocyte release cavity is in a "concave" configuration.

According to the invention, a negative pressure pipe cavity is formed at the end part of the negative pressure suction pipe, which is positioned in the sealing plug, and the tail end of the negative pressure suction pipe is also connected with a negative pressure suction pipe connecting seat for being communicated with a negative pressure device; and the negative pressure tube cavity extends to the tube opening of the sterile collection test tube and is in a concave structure relative to the small-diameter bottom plane of the sealing plug.

According to the invention, the joint of the oocyte release cavity and the cavity of the oocyte collecting tube is also provided with the injector connector, the injector connector is connected with the injection tube, and liquid is injected into the oocyte collecting tube and the puncture needle before ovum fetching to clean the ovum fetching tube.

Preferably, the orifice of the sterile collection test tube is clamped at one third of the upward position of the bottom of the sealing plug (7).

According to the invention, the push injection device for follicle flushing is a constant-temperature push injection pump, and the preheating temperature of the constant-temperature push injection pump is 37+/-1 ℃.

According to the invention, the constant-temperature injection pump comprises a pump shell, a propelling mechanism and an injector fixedly arranged in the pump shell, wherein a push rod is arranged in a needle cylinder of the injector in a sliding way; the pump shell comprises a first chamber and a second chamber which are arranged in parallel front and back; the first chamber and the second chamber are separated by a partition; the injector is arranged at the middle position in the first cavity, the push rod extends into the second cavity, and the push handle at the tail end of the push rod is fixed on the fixed structure in the second cavity; the first chamber is provided with a heating device for preheating, heating and preserving heat of the injector.

According to the invention, the fixing part is a push injection clamping groove formed by extending the inner wall of the pump shell to the central part, and a slideway for automatically sliding the push injection clamping groove in the second cavity is formed in the pump shell; the pushing injection clamping groove automatically slides along the slide way in the second cavity under the action of the pushing mechanism, drives the push rod to push to the depth of the syringe needle cylinder, and accurately controls the pushing speed of the syringe.

According to the invention, the pushing mechanism provides power for the pushing injection clamping groove, so that the push rod which is arranged in the pump shell in a sliding way can reciprocate in the injector; the pushing injection clamping groove is provided with a groove part; the groove part is a peripheral groove formed on the inner wall of the pump shell, and the tail end of the push rod is clamped and fixed in the groove and synchronously moves along with the push injection clamping groove; or the push injection clamping groove is provided with a protruding part; the protruding part is an annular protrusion which is formed towards the tail end of the push rod in sequence, and the tail end of the push rod of the injector is clamped in a groove in the annular protrusion on the outermost layer and synchronously moves along with the push injection clamping groove.

Preferably, the convex part comprises at least three layers of annular convex parts with gradually decreasing areas from inside to outside, and preferably three layers of convex parts.

According to the present invention, the constant temperature bolus pump further comprises a rinse liquid bottle heating tank for preheating the rinse liquid bottle placed at the upper portion thereof; the two heating grooves of the flushing liquid bottle are symmetrically arranged at two sides of the injector heating device respectively. Preferably, a heating wire is paved in the flushing liquid bottle heating groove to heat the flushing liquid bottle.

According to the invention, the invention also comprises an adjusting button arranged on the surface of the shell, a foot switch and a power cord; the adjustment button is configured to adjust a speed of the bolus card slot.

According to the invention, the invention also comprises a puncture needle protecting sleeve and an oocyte collecting tube protecting sleeve; the puncture needle protecting sleeve is used for protecting the puncture needle in the egg taking and packaging process; the oocyte collecting pipe protective sheath sets up in the oocyte collecting pipe with the junction that the puncture needle held the handle, in order to avoid buckling to cause the oocyte collecting pipe blocks up, leads to the oocyte to block or lose.

Preferably, the puncture needle holding handle is cylindrical with a thin middle and thick two ends; the oocyte collecting pipe is composed of a plurality of layers of sleeves, and the layers of sleeves are made of materials with heat exchange media. Preferably, the volume of the flushing channel in the puncture needle cavity is about one eighth to one tenth of the volume of the egg taking channel.

The invention has the beneficial effects that:

1) In the prior art, the ovum directly enters the test tube through the collecting tube, so that the damage to the ovum is relatively large, and the sealing plug is provided with the collecting tube cavity with the convex structure, so that the ovum enters the test tube through the collecting tube cavity, the ovum can drop downwards along the wall of the test tube, the impact force of the ovum when the ovum drops into liquid is reduced, the deformation damage of the ovum is reduced, and the ovum is protected and collected. In addition, the sealing plug of the ovum taking device is also provided with a negative pressure pipe cavity with a concave structure, so that the backflow of liquid in the negative pressure suction process can be prevented, and equipment is protected; and each test tube can be filled with more liquid.

2) The sealing plug of the invention is also designed with an ovum release cavity structure for releasing and buffering the ovum, so that the ovum forms larger droplet balls, the ovum is protected in the falling process, and the ovum is prevented from staying in the pipeline.

3) Because the oocyte taking device sucks out most of oocytes, residual oocytes attached to ovarian tissues are difficult to suck out, and the oocyte collection rate is influenced, the invention designs an oocyte retrieval system aiming at the situation, namely a double-cavity ovum taking device, wherein one cavity is an ovum taking channel, the other cavity is a flushing channel, the flushing channel is connected with a flushing pipe at the handle of a puncture needle, the end part of the flushing pipe is connected with a flushing pipe connecting seat, the flushing pipe connecting seat is communicated with a push-injection flushing device, and when most of oocytes are sucked out, flushing liquid can be pushed into the ovary to flush the residual oocytes, so that the residual oocytes can be sucked out smoothly.

4) The injector push injection flushing device adopts the constant-temperature push injection pump, can provide heating and heat preservation effects for the injector when injecting and flushing residual oocytes, simulates human body environment, ensures that the injector is constant at 37+/-1 ℃ and reduces the influence on ova. And the constant-temperature push pump completely realizes automatic operation, in the process that the push flushing liquid enters the ovary to flush residual oocytes, the pushing speed of the injector is controlled, the flow is indirectly controlled, the egg taking time is shortened, the egg taking process is ensured to be faster and more stable, the phenomena of time and labor waste and the like in the process of flushing the follicles by adopting the hand-push injector through the egg taking needle are avoided, and the pain of patients caused by overlong time in the manual push process is reduced.

Drawings

FIG. 1 is a schematic view of a partial sealing plug of a prior art oocyte retrieval device;

FIG. 2 is a schematic view showing the overall structure of the oocyte retrieval system of the present invention;

FIG. 3 is a schematic view showing a partial structure of a sealing plug of the oocyte retrieval system of the present invention.

Fig. 4 is a schematic view showing the internal structure of the constant temperature bolus pump for follicle irrigation according to the present invention.

Fig. 5 is a schematic view of a part of the structure of the constant temperature bolus pump for follicle irrigation of the present invention.

FIG. 6 is an enlarged view showing the structure of the cross section A-A of the puncture needle of the oocyte retrieval system of the present invention.

In the figure: 1-a puncture needle protecting sleeve; 2-puncture needle; 3-a needle holder; 4-connecting the protective sleeve; 5-collecting pipe; 6-a negative pressure suction tube; 7-sealing plugs; 8-negative pressure suction connecting seats; 9-ovum releasing cavity 10-negative pressure tube cavity; 11-collection tube cavity; 12-syringe connection port; 13-flushing pipe, 14-flushing pipe connecting seat, 101-shell, 201-flushing bottle groove, 301-injector groove, 401-push injection clamping groove, 501-heating device, 601-adjusting button, 701-foot switch, 801-power interface, 901-power switch, 110-inclined port structure, 20-ovum taking channel (main channel), 21-needle core, 22-needle tube, 23-flushing channel (auxiliary channel).

Detailed Description

The following describes embodiments of the invention in detail, but the invention may be practiced in a variety of different ways, as defined and covered by the claims.

When the egg extractor sucks out most of oocytes, residual oocytes attached to ovarian tissues are difficult to suck out, so that the oocyte collection rate is affected, and the invention designs an oocyte retrieval system, namely a double-cavity egg extractor, aiming at the situation, wherein one cavity is an egg extraction channel 20, and the other cavity is a flushing channel 23.

As shown in fig. 2 to 6, the present invention proposes an oocyte retrieval system including a negative pressure aspiration tube 6, and a puncture needle 2, a puncture needle holder 3 and an oocyte collection tube 5 connected in sequence. The sealing plug 7 sets up in the tip of oocyte collection pipeline 5 and negative pressure suction tube 6 and fixes, and oocyte collection pipeline 5 wears out from sealing plug 7 inside, and aseptic collection test tube clamps on sealing plug 7 for collect the oocyte of aspiration. Inside the sealing plug 7, the end of the oocyte collecting pipeline 5 is provided with an ovum releasing cavity 9, and the ovum releasing cavity 9 is communicated with a collecting pipe cavity 11. An egg taking channel 20 (main channel) and a flushing channel 21 (auxiliary channel) are arranged in parallel along the length direction in the cavity of the puncture needle 2. The puncture needle holding handle 3 of the oocyte retrieval system is communicated with a flushing pipe 13 and a flushing pipe connecting seat 14 positioned at one end of the flushing pipe 13, the flushing pipe connecting seat 14 is communicated with a pushing device for follicle flushing, and after most of oocytes are sucked out, flushing liquid can be pushed into the ovary through a flushing channel 23 of the puncture needle to flush residual oocytes so as to suck out the residual oocytes smoothly.

In the prior art, oocytes directly enter a collection test tube through an oocyte collection pipeline, so that the damage to the ovum is relatively large, while the oocyte collection cavity 11 with a protruding structure is arranged in the sealing plug 7, and the protruding structure is provided with a bevel design facing to the outer part, so that the oocytes can drop downwards along the wall of the sterile collection test tube, the impact force when the oocytes drop into liquid is reduced, the deformation damage of the oocytes is reduced, and the oocytes are protected. The sealing plug of the invention is also designed with an oocyte releasing cavity 9 structure for releasing and buffering the ovum, so that the ovum forms a larger droplet ball, the ovum is protected in the falling process, and the ovum is prevented from staying in the pipeline.

In addition, the sealing plug 7 of the invention is also provided with a negative pressure pipe cavity with a concave structure, which can prevent the liquid from flowing backwards in the negative pressure suction process and protect equipment; meanwhile, the negative pressure tube cavity with the concave structure reduces the volume occupied by the negative pressure tube cavity in the sterile collection test tubes, so that each sterile collection test tube is filled with more liquid.

In a preferred embodiment of the invention, as shown in figure 3, the oocyte collection tube cavity 11 extends inwardly of the sterile collection tube and is in a convex configuration with respect to the bottom plane of the sealing plug 7 (the smaller diameter end of the sealing plug); the portion of the oocyte collecting tube cavity 11, which is toward the outside and is close to the tube wall of the sterile collecting tube, is formed with an inclined opening structure 110 to protect the ovum from falling down along the tube wall of the sterile collecting tube connected thereto, and reduce the impact force of the ovum when falling into the liquid, thereby avoiding deformation damage of the ovum. And the collecting pipe cavity 11 can also be used as a collecting pipe for collecting ovum.

Preferably, the inclination angle of the inclined port structure 110 is 20-45 ° with respect to the axial direction of the sealing plug 7, more preferably, the inclination angle is 30-40 °, for example, 15 °,25 °,35 °,45 °,55 °, or any point value in the range of values formed by any two points.

According to the invention, the oocyte release chamber 9 is used for release buffering of the ovum, so that the ovum forms a larger droplet ball, and the ovum is protected from staying in the pipeline during the falling process. Preferably, the connection between the oocyte collecting cavity 11 and the oocyte releasing cavity 9 is in a recessed U-shaped structure.

According to the invention, the end of the negative pressure suction tube 6 positioned in the sealing plug 7 is provided with a negative pressure tube cavity 10 with a larger space, the tail end of the negative pressure suction tube 6 is also connected with a negative pressure suction tube connecting seat 8 which is used for being communicated with a negative pressure absorber, the other end of the negative pressure suction tube connecting seat is connected with the sealing plug 7, and the negative pressure suction tube connecting seat 8 is connected with the negative pressure suction tube 6 and a negative pressure absorbing device. Preferably, the negative pressure aspiration tube 6 is shaped like the oocyte collection tube 5 and is arranged in parallel, both in a "concave" configuration.

The negative pressure tube cavity 10 extends toward the mouth of the sterile collection tube and has a concave shape with respect to the small diameter bottom plane of the sealing plug 7. That is, the bottom end of the oocyte collection tube cavity 11 is below the level of the bottom surface of the sealing plug 7, while the negative pressure tube cavity 10 is above the level of the bottom surface of the sealing plug 7. The invention arranges a negative pressure pipe cavity 10 at the tail end of the negative pressure suction pipe 6, so as to prevent the liquid from flowing backwards in the negative pressure suction process; the negative pressure tube cavity 10 is of a concave design relative to the sealing plug 7, so that the space in the sterile collection test tubes is increased, and each sterile collection test tube can be filled with more flushing liquid.

As shown in fig. 3, a syringe connection port 12 is further formed at the connection part of the oocyte releasing cavity 9 and the oocyte collecting cavity 11, the syringe connection port 12 is connected with a syringe, and cleaning liquid is injected into the oocyte collecting pipeline 5 and the puncture needle 2 before ovum fetching, so as to clean the ovum fetching pipeline.

Preferably, the mouth of the sterile collection tube is snapped into place in the bottom up one third of the sealing plug 7.

As shown in fig. 6, the puncture needle 2 is a double-lumen puncture needle, i.e., a main channel (an egg taking channel 20) for taking out oocytes is formed in a needle core 21, and an auxiliary channel (a flushing channel 23) for flushing residual oocytes by injecting flushing liquid is formed between the needle core 21 and a needle tube 22. One end of the flushing pipe 13 is communicated with the flushing channel 23 through the puncture needle holding handle 3, the other end is connected with a flushing pipe connecting seat 14, and the oocyte collecting pipeline 5 is communicated with the ovum fetching channel 20 through the puncture needle holding handle 3. Preferably, an ultrasonic echo zone with the length of 6-9mm is also arranged at the head end of the puncture needle 2.

According to the invention, the oocyte retrieval system further comprises a puncture needle protecting sleeve 1 and an oocyte collecting pipeline protecting sleeve 4. The puncture needle protective sleeve 1 is used for protecting the puncture needle 2 in the egg taking and packaging process, so that package damage caused by exposure of the puncture needle 2 is avoided, and the product is invalid. The oocyte collecting pipeline protective sleeve 4 is arranged at the joint of the oocyte collecting pipeline 5 and the puncture needle holding handle 3, so that the oocyte collecting pipeline 5 is prevented from being blocked due to bending, and an ovum is prevented from being blocked or lost.

Preferably, the oocyte collecting pipeline 5 is composed of a plurality of layers of sleeves, and the plurality of layers of sleeves are made of materials with heat exchange media so as to ensure that the oocyte collecting pipeline 5 has a heat preservation function. More preferably, the volume of the flushing channel 23 in the cavity of the puncture needle 2 is about one eighth to one tenth of the volume of the aspiration channel 20.

According to the invention, the push injection device for follicle flushing is a constant-temperature push injection pump, and the preheating temperature of the constant-temperature push injection pump is 37+/-1 ℃. Preferably, the constant temperature injection pump comprises a pump shell 101, a pushing mechanism and a syringe 301 fixedly arranged in the pump shell 101, wherein a push rod is arranged in a syringe cylinder of the syringe 301 in a sliding way. The pump shell 101 mainly plays a role in fixing and supporting, the pump shell 101 comprises a first chamber and a second chamber which are arranged in parallel, the first chamber and the second chamber are separated through a partition board, the injector 301 is arranged at the middle position in the first chamber, the push rod extends into the second chamber, and a push handle at the tail end of the push rod is fixed on a fixing piece positioned in the second chamber. A syringe heating device 501 is provided at an intermediate position in the first chamber for preheating, heating and maintaining the temperature of the syringe 301.

Preferably, the fixing part is a push injection clamping groove 401 formed by extending the inner wall of the pump casing 101 towards the central part, a slide way for automatically sliding the push injection clamping groove 401 in the second chamber is formed in the pump casing 101, the push injection clamping groove 401 provides thrust for the injector 301 under the action of a motor, and the push injection clamping groove can automatically slide in the second chamber and drive a push handle of the injector 301 to slide so as to accurately control the pushing speed of the injector 301.

In the invention, the pushing mechanism can provide power for the pushing clamping groove 401, so that a push rod which is arranged in the pump shell 101 in a sliding way reciprocates in the injector 301, the pushing clamping groove 401 automatically slides along a slide way in the second cavity under the action of the pushing mechanism, drives the push rod to push to the deep part of the syringe 301, and precisely controls the pushing speed of the injector 301.

In one embodiment of the present invention, bolus card slot 401 has a groove portion; the groove part is a circumferential groove formed on the inner wall of the pump casing 101, and the end of the push rod is clamped and fixed in the groove. In another embodiment of the present invention, the bolus card slot 401 has a protrusion, which is an annular protrusion formed in sequence facing the end of the push rod, and the end of the push rod is snapped into a groove inside the annular protrusion of the outermost layer. Preferably, the convex part comprises at least three layers of annular convex parts with gradually decreasing areas from inside to outside, and more preferably three layers of convex parts.

According to the present invention, the syringe heating device 501 is a syringe heating groove, and its shape is adapted to the syringe 301. The constant-temperature push injection pump for follicular irrigation can heat and preserve heat of the flushing fluid in the injector when the follicular irrigation is injected and irrigated, simulate the human environment, keep the constant temperature of 37+/-1 ℃ and reduce the influence on ova. Preferably, the area of the injector heating recess covers at least 1/3 of the surface of the injector 301. It is further preferred that the area of the injector heating recess covers at least 1/2 of the surface of the injector 301.

In the present invention, one rinse solution bottle heating tank 201 is provided on each side of the syringe 301, and is configured to heat the rinse solution bottle embedded therein. Preferably, a heating wire is also laid in the rinse solution bottle heating tank 2 for heating the rinse solution bottle so that the preheating temperature thereof is 37 ℃ +/-1 ℃. The constant-temperature injection pump can heat and preserve heat of flushing fluid in the injector when injecting and flushing the follicles, simulate human body environment, keep the constant temperature of 37+/-1 ℃ and reduce the influence on ova. More preferably, the wash bottle heating tank 2 covers 1/4 to 1/2 of the surface area of the wash bottle to ensure that the wash bottle is covered by the heating tank 2 so that the temperature of the wash bottle is maintained at 37 ℃ + -1 ℃.

As shown in fig. 4 and 5, the constant temperature bolus pump further includes an adjusting button 601 provided on the surface of the housing 101, and a foot switch, a power cord, the adjusting button 601 being configured to adjust the speed of the bolus card slot 401. The adjustment button 601 can adjust the push speed of the bolus card slot 401 to adjust the speed of the bolus flushing fluid. Preferably, the puncture needle holder 3 has a cylindrical shape with a thin middle and thick two ends, and the shape is helpful for a doctor to hold the egg taking needle, so that the doctor can operate in operation.

The constant-temperature push injection pump completely realizes automatic operation, in the process that a push injection flushing fluid enters an ovary to flush an ovum, the pushing speed of the injector is controlled, the flow is indirectly controlled, the egg taking time in the egg taking operation is shortened, the egg taking process is ensured to be quicker and more stable, the phenomena of time and labor waste and the like in the process of flushing the ovum by adopting the hand-push injector through the egg taking needle are avoided, and the pain of a patient caused by overlong time in the manual push injection process is reduced.

According to the invention, the propelling mechanism further comprises a stepping motor for providing power, a computer control system for controlling the operation of the stepping motor, a screw rod arranged in parallel with the push rod and a power rod arranged in parallel with the screw rod, wherein the stepping motor is in transmission connection with the screw rod through a reduction gearbox, a screw rod nut in threaded fit with the screw rod is sleeved on the screw rod, one end of the power rod is connected with the screw rod nut, and the other end of the power rod is connected with the push rod; a linear displacement sensor capable of monitoring the running position of the power rod in real time is installed in the pump shell 101, and the linear displacement sensor is electrically connected with a computer control system.

The foot switch has unique structural design, after the constant-temperature injection pump preheats the injector, the injection quantity can be controlled through the foot switch in the process of injecting into the ovary to flush the oocyte, a doctor steps on the foot switch, the constant-temperature injection pump injects flushing fluid, after a certain amount of injection is carried out, the foot switch is stopped in a medical way, and the injector stops injecting the flushing fluid, so that the accuracy and the safety in the operation process are improved.

The constant temperature push injection pump for follicle flushing is connected with the foot switch through an electric wire, a coil spring can be arranged at the upper end of the electric wire, and the lower end of the electric wire is connected with the foot switch and the control switch. One end of the electric wire is arranged on the coil spring, and after the foot switch and the control switch are stored, the electric wire is wound under the action of the coil spring, so that the electric wire is automatically wound in the storage box, and disorder of the electric wire is prevented.

The oocyte retrieval system is mainly used for puncturing follicles to collect ova under the guidance of abdomen or vagina ultrasound, and the specific using method is as follows:

1) Firstly, the sealing plug is connected with a sterile collection test tube, the negative pressure aspirator is connected with a negative pressure absorber connecting seat 8 at the tail end of a negative pressure connecting tube 6, and the sterile test tube is connected with a sealing plug 7.

2) The puncture needle part is fixed on the B ultrasonic vagina probe and is placed into a female vagina (an ultrasonic developing device is arranged at the front end of the puncture needle 2, so that the position of the puncture needle 2 is conveniently observed under the guidance of vaginal ultrasonic waves), at the moment, images of honeycomb ovaries and ultrasonic echo areas at the end part of the puncture needle 1 can be seen on the B ultrasonic display screen, and a doctor can operate the layer puncture needle 2 on the probe according to the images, and under the guidance of vaginal ultrasonic waves, the puncture needle 2 punctures follicles through the vagina. When the puncture needle 2 reaches the surface of the follicle, the puncture needle 2 is accelerated to puncture the cavity of the follicle by moderately applying force after stopping.

3) Confirming that the needle tip is positioned in the follicular cavity, starting the negative pressure aspirator, and sequentially sucking each follicular fluid by using the negative pressure formed by the negative pressure aspirator to obtain the ovum in the follicular until the follicular collapses. After the follicular fluid of one follicle is drained, the puncture needle is inserted into the adjacent larger follicle. If the number of dominant follicles is less than 4-5 or more immature follicles exist, a double-cavity egg taking needle is used, and if necessary, the follicle cavities can be flushed with culture solution for multiple times and repeatedly pumped for 2-3 times; if the number of dominant follicles exceeds 4-5 available single-cavity ovum pick-up devices (ovum pick-up devices without flushing devices), in the ovum pick-up process, if the number of the ovum pick-up devices is found to be less than the number of the follicles seen under the ultrasound, the double-cavity ovum pick-up devices are used, and each follicle is flushed 2-3 times by using culture solution (i.e. flushing liquid) so as to acquire more oocytes and avoid losing. The sucked follicular fluid enters the oocyte collecting pipeline through the ovum taking pipeline of the inner cavity of the puncture needle, and finally reaches the sterile collecting test tube.

4) When the residual oocyte attached to the ovarian tissue is difficult to suck out, the flushing liquid can be injected into the flushing channel of the puncture needle by using the injector pushing and flushing device and reaches the inside of the ovary, the sucking process is repeated, and the residual oocyte is continuously pumped out. After the ovum taking is finished, the puncture needle 2 is withdrawn outside the body. The pelvis was routinely scanned to check for possible internal bleeding or hematoma formation.

The above description is only of the preferred embodiments of the present invention and is not intended to limit the present invention, but various modifications and variations can be made to the present invention by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. An oocyte retrieval system is characterized by comprising a negative pressure suction tube (6), a puncture needle (2), a puncture needle holding handle (3) and an oocyte collection pipeline (5) which are connected in sequence; the end parts of the oocyte collecting pipeline (5) and the negative pressure suction pipe (6) are fixed inside a sealing plug (7), the oocyte collecting pipeline (5) penetrates out of the sealing plug (7), and the sterile collecting test pipe is clamped on the sealing plug (7) and is used for collecting the sucked oocyte;

an oocyte release cavity (9) is arranged at the tail end of the oocyte collection pipeline (5) inside the sealing plug (7), and the oocyte release cavity (9) is communicated with an oocyte collection cavity (11);

an egg taking channel (20) and a flushing channel (23) are arranged in parallel in the length direction of the inner cavity of the puncture needle (2);

the puncture needle holding handle (3) of the oocyte retrieval system is communicated with a flushing pipe (13) and a flushing pipe connecting seat (14) positioned at one end of the flushing pipe (13), and the flushing pipe connecting seat (14) is communicated with a push injection device for follicle flushing so as to push and inject flushing fluid into the ovary through a flushing channel (23) of the puncture needle (2) to flush residual oocytes and suck the residual oocytes.

2. Oocyte retrieval system according to claim 1, characterized in that the oocyte collection tube cavity (11) extends towards the inside of the sterile collection tube and is in a convex configuration with respect to the small diameter bottom plane of the sealing plug (7); the portion of the oocyte collecting cavity (11) which faces the outer side and is close to the tube wall of the sterile collecting tube is provided with an inclined opening structure (110) so as to prevent the ovum from falling downwards along the tube wall of the sterile collecting tube connected with the oocyte collecting cavity.

3. Oocyte retrieval system according to claim 2, characterized in that the inclined port structure (110) is inclined at an angle of 20-45 ° with respect to the axial direction of the sealing plug (7).

4. An oocyte retrieval system according to claim 3, wherein the angle of inclination is 30-40 °.

5. Oocyte retrieval system according to claim 2, characterized in that the inclined angle of the inclined port structure (110) is 15 °,25 °,35 °,45 °,55 ° or any point value in the range of values comprised by any two points.

6. An oocyte retrieval system according to claim 1, wherein the connection of the oocyte collection cavity (11) with the oocyte release cavity (9) is in a "concave" configuration.

7. Oocyte retrieval system according to claim 1, characterized in that the end of the negative pressure aspiration tube (6) inside the sealing plug (7) is formed with a negative pressure tube cavity (10), the end of the negative pressure aspiration tube (6) is further connected with a negative pressure aspiration tube connection seat (8) for connection with a negative pressure device;

the negative pressure tube cavity (10) extends to the tube opening of the sterile collection test tube and is in a concave structure relative to the small-diameter bottom plane of the sealing plug (7).

8. Oocyte retrieval system according to claim 1, characterized in that the connection of the oocyte release cavity (9) and the oocyte collection tube cavity (11) is also provided with a syringe connection port (12), the syringe connection port (12) is connected with a syringe, and the liquid is injected into the oocyte collection tube (5) and the puncture needle (2) before the aspiration to clean the aspiration tube.

9. Oocyte retrieval system according to claim 1, characterized in that the orifice of the sterile collection tube is snapped in the bottom of the sealing plug (7) in the upward third position.

10. An oocyte retrieval system according to claim 1, wherein the push device for follicle irrigation is a constant temperature push pump with a pre-heating temperature of 37 ℃ ± 1 ℃.

11. The oocyte retrieval system according to claim 10, wherein the constant temperature bolus pump includes a pump housing (101), a propulsion mechanism, and a syringe (301) fixedly disposed within the pump housing (101), a push rod being slidingly disposed within a barrel of the syringe (301); the pump shell (101) comprises a first chamber and a second chamber which are arranged in parallel front and back; the first chamber and the second chamber are separated by a partition; the injector (301) is arranged at the middle position in the first cavity, the push rod extends into the second cavity, and the push handle at the tail end of the push rod is fixed on the fixing piece in the second cavity;

the first chamber is provided with heating means (501) for preheating, heating and insulating the syringe (301).

12. The oocyte retrieval system according to claim 11, wherein the fixing member is a push injection slot (401) formed by extending the inner wall of the pump case (101) toward the center, and a slide way for automatically sliding the push injection slot (401) in the second chamber is formed in the pump case (101); the pushing injection clamping groove (401) automatically slides along a slide way in the second cavity under the action of the pushing mechanism, drives the push rod to push to the depth of a syringe (301) needle cylinder, and accurately controls the pushing speed of the syringe (301).

13. An oocyte retrieval system according to claim 12, wherein the pushing mechanism provides power to the bolus catch (401) to reciprocate a slidably disposed plunger within the pump housing (101) into the syringe (301);

the push card slot (401) is provided with a groove part; the groove part is a circumferential groove formed on the inner wall of the pump shell (101), and the tail end of the push rod is clamped and fixed in the groove and synchronously moves along with the push injection clamping groove (401); or alternatively

The push injection clamping groove (401) is provided with a protruding part; the protruding part is an annular protrusion which is formed towards the tail end of the push rod in sequence, and the tail end of the push rod of the injector (301) is clamped in a groove in the annular protrusion on the outermost layer and synchronously moves along with the pushing injection clamping groove (401).

14. An oocyte retrieval system according to claim 13, wherein the boss includes at least three annular protrusions of progressively decreasing area from inside to outside.

15. An oocyte retrieval system according to claim 11, further comprising a rinse solution bottle heating tank (201) for preheating the rinse solution bottle placed in the upper portion thereof; the two flushing liquid bottle heating tanks (201) are symmetrically arranged on two sides of the heating device (501) respectively.

16. Oocyte retrieval system according to claim 15, wherein a heating wire is laid in the rinse solution bottle heating tank (201) to heat the rinse solution bottle.

17. The oocyte retrieval system according to claim 12, further comprising an adjustment button (601) provided on the surface of the pump housing (101), and a foot switch, a power cord; the adjustment button (601) is configured to adjust the speed of the bolus card slot (401).

18. The oocyte retrieval system according to claim 1, characterized by further comprising a needle protection sheath (1) and an oocyte collection tube protection sheath (4); the puncture needle protection sleeve (1) is used for protecting the puncture needle (2) in the egg taking and packaging process; the oocyte collecting pipe protective sleeve (4) is arranged at the joint of the oocyte collecting pipeline (5) and the puncture needle holding handle (3), so that the phenomenon that the oocyte collecting pipeline (5) is blocked due to bending is avoided, and oocytes are blocked or lost.

19. Oocyte retrieval system according to claim 1, characterized in that the needle holding handle (3) is cylindrical with thin middle and thick ends; the oocyte collecting pipeline (5) is composed of a plurality of layers of sleeves, and the layers of sleeves are made of materials with heat exchange media.

20. Oocyte retrieval system according to claim 1, characterized in that the volume of the flushing channel (23) in the lumen of the needle (2) is one eighth to one tenth of the volume of the aspiration channel (20).