CN114236800A - Micro-distance self-immersion type microscopic imaging system and microscopic imaging method thereof - Google Patents
Micro-distance self-immersion type microscopic imaging system and microscopic imaging method thereof Download PDFInfo
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- CN114236800A CN114236800A CN202111575180.2A CN202111575180A CN114236800A CN 114236800 A CN114236800 A CN 114236800A CN 202111575180 A CN202111575180 A CN 202111575180A CN 114236800 A CN114236800 A CN 114236800A
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- 238000007654 immersion Methods 0.000 title claims abstract description 69
- 238000003384 imaging method Methods 0.000 title claims abstract description 55
- 239000007788 liquid Substances 0.000 claims abstract description 21
- 238000005286 illumination Methods 0.000 claims abstract description 6
- 238000000034 method Methods 0.000 claims abstract description 6
- 230000004907 flux Effects 0.000 claims abstract description 3
- 238000000386 microscopy Methods 0.000 claims description 10
- 230000005499 meniscus Effects 0.000 claims description 9
- 239000000463 material Substances 0.000 claims description 8
- 238000003325 tomography Methods 0.000 claims description 3
- 244000005700 microbiome Species 0.000 abstract description 6
- 238000004587 chromatography analysis Methods 0.000 abstract description 4
- 230000008569 process Effects 0.000 abstract description 4
- 238000010586 diagram Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 4
- 239000003814 drug Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000003287 optical effect Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 210000000582 semen Anatomy 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000003670 easy-to-clean Effects 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000009182 swimming Effects 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/02—Objectives
- G02B21/025—Objectives with variable magnification
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/33—Immersion oils, or microscope systems or objectives for use with immersion fluids
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/361—Optical details, e.g. image relay to the camera or image sensor
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Abstract
The invention provides a microspur self-immersion microscopic imaging system, which comprises an immersion lens, a lens group, a diaphragm, a front fixed lens group, a zoom lens group, a compensation lens group, a focusing lens group and a camera, wherein the immersion lens is arranged in the front fixed lens group; the immersion lens is used for being in immersion contact with a liquid environment where the sample is located; the diaphragm is used for changing the luminous flux; the illumination light path sequentially passes through the immersion lens, the lens group, the diaphragm, the front fixed lens group, the zoom lens group, the compensation lens group and the focusing lens group and is converged at the camera; the front fixed lens group is used for imaging the image of the lens group to an object plane required by the zoom lens group. The structure can be used for observing living cells or microorganisms, chromatography observation can be performed, the observation data volume is increased, the reliability is improved, and the stability of the liquid level in the chromatography observation process is guaranteed.
Description
Technical Field
The invention belongs to the technical field of microscopic imaging, and particularly relates to a microspur self-immersion type microscopic imaging system and a microscopic imaging method thereof.
Background
Microscopes are widely used in various industries such as biology and medicine, and are used in medicine to observe forms of bacteria, cells, microorganisms, and the like, which cannot be distinguished by naked eyes. In many microscopes used for biological observation, slide assistance is required to observe cells or microorganisms attached to a layer near the slide, and this operation method is likely to cause a decrease in activity of an observation target when the observation target comes into contact with the slide or air, and to affect the reliability of the observation result. Many living cells and motile objects in medicine exhibit strong activity in their own living environment, and easily lose or lose activity after contacting air or other media, so that their survival environment is ensured during observation. Many motile microorganisms or cells survive in a liquid environment and require a microscope objective lens to be able to infiltrate the liquid. However, many liquids have low transparency and the working distance of the microscope is long, and the low transparency liquid in the working distance influences the imaging definition of the microscope.
The size of many observed targets is very small, and mobility is very strong, needs to increase the data volume of gathering to the target observation of different aspect, adopts statistical method to carry out data processing, improves observation result reliability.
Disclosure of Invention
The invention provides a microspur self-immersion microscopic imaging system and a microscopic imaging method thereof in order to overcome the defects in the prior art and realize clear microscopic imaging of a multilayer target in a liquid environment. In order to achieve the purpose, the invention adopts the following specific technical scheme:
in a first aspect, the present invention provides a macro self-immersion microscopy imaging system, comprising an immersion lens, a lens assembly, a diaphragm, a front fixed lens assembly, a zoom lens assembly, a compensation lens assembly, a focusing lens assembly and a camera; the immersion lens is used for being in immersion contact with a liquid environment where the sample is located; the diaphragm is used for changing the luminous flux; the illumination light path sequentially passes through the immersion lens, the lens group, the diaphragm, the front fixed lens group, the zoom lens group, the compensation lens group and the focusing lens group and is converged at the camera; the front fixed lens group is used for imaging the image of the lens group to an object plane required by the zoom lens group.
Preferably, the immersion lens is a biconvex lens, the focal length is 2.2 mm-3.2 mm, the refractive index range of the material is 1.73-1.75, and the Abbe number range is 43-45.
Preferably, the lens group is used for integrating light entering the immersion lens to a diaphragm, the combined focal length of the lens group is 8.0-10 mm, the material refractive index range of the lens group is sequentially 1.73-1.75, 1.74-1.76 and 1.6-1.65, and the Abbe number range of the lens group is sequentially 43-46, 25-30 and 52-58.
Preferably, the front fixed lens group is a meniscus lens, the focal length is 22 mm-30 mm, the refractive index range is 1.75-1.82, and the Abbe number range is 23-25.
Preferably, the zoom lens group is a biconvex positive lens, the focal length is 30-40 mm, the refractive index range is 1.45-1.5, and the Abbe number range is 77-82.
Preferably, the compensation lens group is a meniscus lens, the focal length is 65-74 mm, the refractive index range is 1.75-1.82, and the Abbe number range is 23-25.
Preferably, the focal length of the focusing mirror group is 35-43 mm, the refractive index range is 1.65-1.72, and the Abbe number range is 53-58.
Preferably, the system has an object-side chromatographic observation range of ± 0.02 mm.
In a second aspect, the present invention also provides a macro self-immersion microscopy imaging method, comprising the steps of:
step 2, placing the target sample in a sample cell, and placing the sample cell on a sample platform;
and 3, contacting the target sample in the sample pool with an immersion lens of the microspur self-immersion type microscopic imaging system, starting a light source to illuminate the target sample, and collecting light illuminating the target sample in the camera through the immersion lens, a lens group, a diaphragm, a front fixed lens group, a zoom lens group, a compensation lens group and a focusing lens group in sequence.
Preferably, the light source is selected from polychromatic light or monochromatic light; when the light source is monochromatic light, the wave band range is 470 nm-630 nm.
The invention can obtain the following technical effects:
according to the invention, by designing the microspur self-immersion type chromatography zooming microscopic imaging structure, the working distance of the objective lens under the structure is very small, the influence of a low-transparency liquid environment in the working distance on imaging is small, and meanwhile, the objective lens is in self-immersion contact with the liquid environment, so that the objective lens can be used for observing living cells or microorganisms, and can be used for chromatographic observation, the observation data volume is increased, the reliability is improved, and the stability of the liquid level in the chromatographic observation process is ensured. In addition, in order to be suitable for the requirements of various resolutions and various magnifications, the microscope objective is designed into a zoom structure, the zoom structure is axial zoom, the switching process of the objective when the microscope objective is used at different magnifications is omitted, and the system structure is simplified.
Drawings
FIG. 1 is an optical path diagram of a macro self-immersion microscopy imaging system according to an embodiment of the present invention;
FIG. 2 is an optical path diagram of a macro self-immersion microscopy imaging system according to an embodiment of the present invention;
fig. 3 is an optical path diagram of a macro self-immersion microscopy imaging system according to an embodiment of the invention.
Reference numerals:
the zoom lens comprises an immersion lens 1, a lens group 2, a diaphragm 3, a front fixed lens group 4, a zoom lens group 5, a compensation lens group 6, a focusing lens group 7 and a camera 8.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail below with reference to the accompanying drawings and specific embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not to be construed as limiting the invention.
The invention aims to provide a microspur self-immersion type microscopic imaging system and an imaging method thereof. The following will describe a macro self-immersion type microscopic imaging and its imaging method provided by the present invention in detail through specific embodiments.
Fig. 1 is a light path diagram of a macro self-immersion microscopic imaging system according to an embodiment of the present invention, where an illumination light path sequentially passes through an immersion lens 1, a lens group 2, a diaphragm 3, a front fixed lens 4, a zoom lens group 5, a compensation lens group 6, and a focusing lens group 7 to converge on a camera 8, the zoom lens group 5 is a double convex positive lens, the compensation lens group 6 is a meniscus lens, the zoom lens group 5 abuts against the compensation lens group 6, the zoom lens group 5 and the front fixed lens 4 are distributed at intervals, and the compensation lens group 6 and the focusing lens group 7 are distributed at intervals.
Preferably, the immersion lens is a biconvex lens, the focal length is 2.2-3.2 mm, the refractive index range of the material is 1.73-1.75, and the Abbe number range is 43-45.
Preferably, the lens group is used for integrating light entering the immersion lens to a diaphragm, the combined focal length of the lens group is 8.0-10 mm, the material refractive index range of the lens group is sequentially 1.73-1.75, 1.74-1.76 and 1.6-1.65, and the Abbe number range of the lens group is sequentially 43-46, 25-30 and 52-58.
Preferably, the front fixed lens group is a meniscus lens, the focal length is 22 mm-30 mm, the refractive index range is 1.75-1.82, and the Abbe number range is 23-25.
Preferably, the zoom lens group is a biconvex positive lens, the focal length is 30-40 mm, the refractive index range is 1.45-1.5, and the Abbe number range is 77-82.
Preferably, the compensation lens group is a meniscus lens, the focal length is 65-74 mm, the refractive index range is 1.75-1.82, and the Abbe number range is 23-25.
Preferably, the focal length of the focusing mirror group is 35-43 mm, the refractive index range is 1.65-1.72, and the Abbe number range is 53-58.
Preferably, the system has an object-side chromatographic observation range of ± 0.02 mm.
In a second aspect, the present invention also provides a macro self-immersion microscopy imaging method, comprising the steps of:
step 2, placing the target sample in a sample cell, and placing the sample cell on a sample platform;
and 3, contacting the target sample in the sample pool with an immersion lens of the microspur self-immersion type microscopic imaging system, starting a light source to illuminate the target sample, and collecting light illuminating the target sample in the camera through the immersion lens, a lens group, a diaphragm, a front fixed lens group, a zoom lens group, a compensation lens group and a focusing lens group in sequence.
Preferably, the light source is selected from polychromatic light or monochromatic light; when the light source is monochromatic light, the wave band range is 470 nm-630 nm.
Fig. 2 is a light path diagram of a macro self-immersion microscope imaging system according to an embodiment of the present invention, wherein the illumination light path sequentially passes through an immersion lens 1, a lens group 2, a diaphragm 3, a front fixed lens 4, a zoom lens group 5, a compensating lens group 6, a focusing lens group 7, converges at a camera 8, the zoom lens group 5 is a biconvex positive lens, the compensation lens group 6 is a meniscus lens, the zoom lens group 5 and the compensation lens group 6 are distributed at intervals, the zoom lens group 5 and the front fixed lens 4 are distributed at intervals, the compensation lens group 6 and the focusing lens group 7 are distributed at intervals, the interval between the variable-power mirror group 5 and the compensating mirror group 6 is smaller than the interval between the variable-power mirror group 5 and the front fixed mirror 4, the distance between the zoom lens group 5 and the compensation lens group 6 is smaller than the distance between the compensation lens group 6 and the focusing lens group 7.
FIG. 3 is a light path diagram of a macro self-immersion microscope imaging system according to an embodiment of the present invention, the illumination light path sequentially passes through an immersion lens 1, a lens group 2, a diaphragm 3, a front fixed lens 4, a zoom lens group 5, a compensating lens group 6, a focusing lens group 7, converges at a camera 8, the zoom lens group 5 is a biconvex positive lens, the compensation lens group 6 is a meniscus lens, the zoom lens group 5 and the compensation lens group 6 are distributed at intervals, the zoom lens group 5 and the front fixed lens 4 are distributed at intervals, the compensation lens group 6 and the focusing lens group 7 are distributed at intervals, the interval between the variable-power mirror group 5 and the compensating mirror group 6 is larger than the interval between the variable-power mirror group 5 and the front fixed mirror 4, the distance between the zoom lens group 5 and the compensation lens group 6 is smaller than the distance between the compensation lens group 6 and the focusing lens group 7.
The invention has the main structure that a microscope is soaked in a liquid environment where an observation target belongs, after an active target near an immersion lens is illuminated by a light source, light enters a macro microscope objective, and is magnified and imaged on a camera through the immersion lens, a fixed lens group, a zoom lens group and a chromatographic lens group, and the characteristics of the target, such as the swimming characteristics of sperms in semen, can be observed in real time by adjusting the exposure time of the camera. The object space numerical aperture of the microspur self-immersion type chromatography zoom microscopic imaging structure corresponds to the magnification and the object space view field, and the high magnification structure corresponds to the large numerical aperture and the small view field. The zoom structure is axial zoom, the switching of different magnifications is realized by adopting the structure of an axial moving lens group, the zoom lens group, the compensation lens group and the fixed lens group are included, and the movement of the chromatographic lens group is used for realizing the observation of different layer targets and the focusing of clear image surface imaging.
The invention adopts the microspur self-immersion zoom microscopic imaging technology, solves the problem of difficulty in observing active cells or motile microorganisms in a liquid environment, enables the microscope objective to be directly immersed in the liquid environment by utilizing the self-immersion imaging technology, enables the immersion lens to be suitable for most liquid environments such as blood, semen, urine and the like, and is easy to clean. Meanwhile, the micro-distance imaging technology is adopted, the problem that the long-working-distance objective lens cannot clearly image in low-transparency liquid is solved, and the micro-distance imaging objective lens can be used in a liquid environment with high transparency and a liquid environment with low transparency. The microscope objective is designed into a tomography structure, and the imaging object plane is changed along with the movement of a focusing lens in the objective, so that the imaging of different liquid level targets is realized, and the stability of the liquid level in the tomography observation process is improved. The microscope imaging system is provided with an axial zoom structure, the problem that the traditional microscope needs to switch the objective lens when used at different magnifications is solved, the system structure is simplified, the system size is reduced, and the use requirements of various magnifications can be met.
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.
Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art within the scope of the present invention.
The above embodiments of the present invention should not be construed as limiting the scope of the present invention. Any other corresponding changes and modifications made according to the technical idea of the present invention should be included in the protection scope of the claims of the present invention.
Claims (10)
1. A microspur self-immersion microscopic imaging system is characterized by comprising an immersion lens, a lens group, a diaphragm, a front fixed lens group, a zoom lens group, a compensation lens group, a focusing lens group and a camera; the immersion lens is used for being in immersion contact with a liquid environment where the sample is located; the diaphragm is used for changing the luminous flux; the illumination light path sequentially passes through the immersion lens, the lens group, the diaphragm, the front fixed lens group, the zoom lens group, the compensation lens group and the focusing lens group and is converged at the camera; the front fixed lens group is used for imaging the image of the lens group to an object plane required by the zoom lens group.
2. The macro self-immersion microscopic imaging system according to claim 1, wherein the immersion lens is a biconvex lens, the focal length is 2.2mm to 3.2mm, the refractive index of the material is in the range of 1.73 to 1.75, and the abbe number is in the range of 43 to 45.
3. The macro self-immersion microscopy imaging system according to claim 1, wherein the lens group is used for integrating light entering the immersion lens to the diaphragm, the combined focal length of the lens group is 8.0mm to 10mm, the refractive index of the material of the lens group is in the range of 1.73 to 1.75, 1.74 to 1.76 and 1.6 to 1.65 in sequence, and the Abbe number of the lens group is in the range of 43 to 46, 25 to 30 and 52 to 58 in sequence.
4. The macro self-immersion microscopic imaging system according to claim 1, wherein the front fixed lens group is a meniscus lens, the focal length is 22mm to 30mm, the refractive index is 1.75 to 1.82, and the abbe number is 23 to 25.
5. The macro self-immersion microscopic imaging system according to claim 1, wherein the variable power lens group is a biconvex positive lens, the focal length is 30mm to 40mm, the refractive index is in the range of 1.45 to 1.5, and the Abbe number is in the range of 77 to 82.
6. The macro self-immersion microscopic imaging system according to claim 1, wherein the compensating lens group is a meniscus lens, the focal length is 65-74 mm, the refractive index is 1.75-1.82, and the Abbe number is 23-25.
7. The macro self-immersion microscope imaging system according to claim 1, wherein the focal length of the focusing lens group is 35mm to 43mm, the refractive index is 1.65 to 1.72, and the abbe number is 53 to 58.
8. The macro self-immersion microscopy imaging system according to claim 1, wherein the system has an object-side tomography observation range of ± 0.02 mm.
9. A microspur self-immersion type microscopic imaging method is characterized by comprising the following steps:
step 1, calibrating a macro self-immersion microscopy imaging system according to any one of claims 1-8;
step 2, placing the target sample in a sample cell, and placing the sample cell on a sample platform;
and 3, contacting the target sample in the sample pool with an immersion lens of the microspur self-immersion type microscopic imaging system, starting a light source to illuminate the target sample, and collecting light illuminating the target sample in the camera through the immersion lens, a lens group, a diaphragm, a front fixed lens group, a zoom lens group, a compensation lens group and a focusing lens group in sequence.
10. The method of macro self-immersion microscopy imaging according to claim 9, wherein the light source is selected from polychromatic or monochromatic light; when the light source is monochromatic light, the wave band range is 470 nm-630 nm.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115855454A (en) * | 2023-02-10 | 2023-03-28 | 武汉加特林光学仪器有限公司 | Detection device and method for near-to-eye display products adaptive to different diopters |
| JP7467754B1 (en) | 2023-06-19 | 2024-04-15 | ジョウシュウシ エーエーシー レイテック オプトロニクス カンパニーリミテッド | Microscope Objectives |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112526737A (en) * | 2021-01-18 | 2021-03-19 | 麦克奥迪实业集团有限公司 | Immersion liquid microobjective |
| CN113376820A (en) * | 2021-03-22 | 2021-09-10 | 季华实验室 | Large-magnification microscopic imaging optical system and optical device |
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2021
- 2021-12-21 CN CN202111575180.2A patent/CN114236800A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112526737A (en) * | 2021-01-18 | 2021-03-19 | 麦克奥迪实业集团有限公司 | Immersion liquid microobjective |
| CN113376820A (en) * | 2021-03-22 | 2021-09-10 | 季华实验室 | Large-magnification microscopic imaging optical system and optical device |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115855454A (en) * | 2023-02-10 | 2023-03-28 | 武汉加特林光学仪器有限公司 | Detection device and method for near-to-eye display products adaptive to different diopters |
| JP7467754B1 (en) | 2023-06-19 | 2024-04-15 | ジョウシュウシ エーエーシー レイテック オプトロニクス カンパニーリミテッド | Microscope Objectives |
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