CN114231426A - Compound microbial agent and application thereof - Google Patents
Compound microbial agent and application thereof Download PDFInfo
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- CN114231426A CN114231426A CN202210073732.8A CN202210073732A CN114231426A CN 114231426 A CN114231426 A CN 114231426A CN 202210073732 A CN202210073732 A CN 202210073732A CN 114231426 A CN114231426 A CN 114231426A
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- ginseng
- microbial agent
- microbial
- viable bacteria
- bacillus amyloliquefaciens
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention relates to a compound microbial agent and application thereof, belonging to the technical field of biocontrol microbial agents. The compound microbial agent comprises a paecilomyces lilacinus microbial agent and a bacillus amyloliquefaciens microbial agent; the ratio of viable bacteria contained in the paecilomyces lilacinus microbial inoculum to viable bacteria contained in the bacillus amyloliquefaciens microbial inoculum is 1-5: 10 to 50. The prevention and treatment effect of the compound microbial agent for preventing and treating the rust rot of ginseng and American ginseng caused by the bacillus can reach more than 85 percent.
Description
Technical Field
The invention relates to the technical field of biocontrol microbial agents, and particularly relates to a compound microbial agent and application thereof.
Background
Cylindrocarpon, belonging to the subdivision Deuteromycotina, class Hyphomycetes, order Hyphomycetales, family Conidiobolus, genus Cylindrocarpon, is a common soil parasitic bacterium, which is mainly parasitic in some fruit trees, forest trees, flowers and medicinal plants. The host of the cylindrosporium is different, the harm degree of the cylindrosporium to crops is different, the harm degree of the cylindrosporium host on the crops and vegetables is not serious, and when the cylindrosporium host is on ginseng and American ginseng, the cylindrosporium host can cause rust rot and has high morbidity, and the yield and the quality of various ginseng are seriously influenced.
The prior art mainly aims at preventing and treating the rust rot caused by the staurosporium by chemical prevention and pesticide prevention, and the prevention and treatment methods have the following defects: pollutes the environment, is easy to generate drug resistance, influences the quality of the ginseng, and the like. The biological control has the advantages of no pollution, no drug resistance and no influence on the quality of crops, but the control effect of the existing biological agent for controlling the rust rot is still not ideal and needs to be further improved.
Disclosure of Invention
The invention aims to provide a compound microbial agent and application thereof.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a compound microbial agent, which comprises a paecilomyces lilacinus microbial agent and a bacillus amyloliquefaciens microbial agent;
the ratio of viable bacteria contained in the paecilomyces lilacinus microbial inoculum to viable bacteria contained in the bacillus amyloliquefaciens microbial inoculum is 1-5: 10 to 50.
Preferably, the lilac pseudo-blueThe viable count of the fungus agent is 2 multiplied by 109～1×1010cfu/g。
Preferably, the number of viable bacteria contained in the Bacillus amyloliquefaciens preparation is 2X 1010～1×1011cfu/g。
The invention also provides application of the compound microbial agent in preventing and treating plant diseases caused by the bacillus.
Preferably, the plant comprises ginseng or American ginseng.
Preferably, the plant disease comprises rust rot.
Preferably, the application method of the compound microbial agent in preventing and treating ginseng, American ginseng or red sage root rust rot caused by the bacillus is soil-accompanying treatment.
Preferably, the clay-accompanying treatment method comprises: 1-2 kg of compound microbial agent is added to each cubic meter of soil.
The invention provides a compound microbial agent and application thereof. When the paecilomyces lilacinus microbial inoculum and the bacillus amyloliquefaciens microbial inoculum are matched to prevent and treat the rust rot caused by the column spore fungi, the two bacteria can act synergistically, and the prevention and treatment effect is better than that when the two bacteria are used independently.
The compound microbial agent is safe, pollution-free, free of drug resistance and free of any influence on the quality of ginseng. Can also improve the yield and quality of ginseng.
Detailed Description
The invention provides a compound microbial agent, which comprises a paecilomyces lilacinus microbial agent and a bacillus amyloliquefaciens microbial agent;
the ratio of viable bacteria contained in the paecilomyces lilacinus microbial inoculum to viable bacteria contained in the bacillus amyloliquefaciens microbial inoculum is 1-5: 10 to 50.
In the invention, the ratio of viable bacteria in the paecilomyces lilacinus microbial inoculum to viable bacteria in the bacillus amyloliquefaciens microbial inoculum is preferably 2-4: 20-40, and more preferably 3: 30.
In the present invention, the number of viable bacteria contained in the paecilomyces lilacinus microbial inoculum is 2X 109～1×1010cfu/g, preferably 6X 109cfu/g。
In the invention, the number of viable bacteria contained in the bacillus amyloliquefaciens microbial inoculum is 2 multiplied by 1010～1×1011cfu/g, preferably 6X 1010cfu/g。
The invention also provides application of the compound microbial agent in preventing and treating plant diseases caused by the bacillus.
In the present invention, the plant includes ginseng or American ginseng.
In the present invention, the plant disease includes rust rot.
In the invention, the compound microbial agent is used by soil-accompanying treatment.
In the present invention, the clay-accompanying treatment is: 1-2 kg of compound microbial agent is added to each cubic meter of soil, and 1.5kg is preferred.
The embodiments of the present invention will be described in detail with reference to the following examples, but they should not be construed as limiting the scope of the present invention.
The paecilomyces lilacinus microbial inoculum in the embodiment of the invention is purchased from Jinnanbi commercial and trade company Limited.
The bacillus amyloliquefaciens preparation described in the examples of the present invention was purchased from bekken baoding regu biotechnology limited.
In the examples and comparative examples of the present invention, the disease index [ Σ (the disease level representative value × the number of plants at that level)/(the disease most serious level representative value × the total number of plants) ] × 100;
the incidence grades of the ginseng rust rot in the examples and the comparative examples of the invention are divided into 5 grades: grade 0 indicates that the ginseng root has no lesion; grade 1 indicates that the surface area of the lesion of the ginseng root is less than 25%; grade 2 represents that the surface area of the ginseng root scabs is 26-50%; grade 3 represents that the surface area of the ginseng root scabs is 51-75%; grade 4 indicates that the lesion of ginseng root occupies more than 76% of the surface area or completely decays.
In the examples of the present invention and the comparative examples, the control effect is [ (control disease index-treatment disease index)/control disease index ] × 100%.
The ginseng variety used in the examples and comparative examples of the present invention was ginseng radix et rhizoma.
The planting soils with the incidence index of ginseng rust rot of more than 50% in the Changbai mountain ginseng planting bases described in the examples and comparative examples of the present invention are the planting soils taken from different places of the Changbai mountain ginseng planting bases, and the planting soils are mixed and used in the examples and comparative examples.
Randomly sampling the mixed planting soil, and screening microorganisms in the planting soil to obtain microorganisms and pillar fungi which are mainly including pillar fungus Cylindrocarpon destructors Zinns, pillar fungus Cylindrocarpon anacericola and pillar fungus Cylindrocarpon destructors.
Example 1
Taking 0.5kg of paecilomyces lilacinus microbial inoculum and 0.5kg of bacillus amyloliquefaciens microbial inoculum to be uniformly mixed to obtain the compound microbial inoculum. The number of viable bacteria contained in the paecilomyces lilacinus microbial inoculum is 1 multiplied by 1010cfu/g, the number of viable bacteria contained in the bacillus amyloliquefaciens microbial inoculum is 1 multiplied by 1011cfu/g。
Taking 1 cubic meter of planting soil with the incidence index of ginseng rust rot more than 50% in a Changbai mountain ginseng planting base, adding the compound microbial agent into the planting soil to obtain soil to be planted, and paving the soil to be planted in a planting pot with the thickness of 40 cm. Taking 2-year-old ginseng seedlings, disinfecting by sodium hypochlorite, transplanting into a planting pot paved with soil to be planted, keeping the plant spacing at 15cm, and conventionally culturing. And (4) observing the morbidity of the ginseng rust rot during harvesting, and calculating the disease index. The results are shown in Table 1.
The method comprises the steps of directly planting 2-year-old ginseng seedlings in planting soil with the incidence index of ginseng rust rot more than 50% in a Changbai mountain ginseng planting base as a control, observing the incidence condition of the ginseng rust rot during harvesting, and calculating the disease index. The results are shown in Table 1. The control effect of the treatment group added with the compound microbial agent in example 1 on the rust and rot is calculated. The results are shown in Table 1.
The fresh weight of each individual plant of ginseng was weighed in the treatment group with the complex microbial inoculant of example 1 and the control group without the complex microbial inoculant treatment. The results are shown in Table 2.
Example 2
Taking 0.5kg of paecilomyces lilacinus microbial inoculum and 0.5kg of bacillus amyloliquefaciens microbial inoculum to be uniformly mixed to obtain the compound microbial inoculum. The number of viable bacteria contained in the paecilomyces lilacinus microbial inoculum is 6 multiplied by 109cfu/g, the number of viable bacteria contained in the bacillus amyloliquefaciens microbial inoculum is 2 multiplied by 1010cfu/g。
Taking 1 cubic meter of planting soil with the incidence index of ginseng rust rot more than 50% in a Changbai mountain ginseng planting base, adding the compound microbial agent into the planting soil to obtain soil to be planted, and paving the soil to be planted in a planting pot with the thickness of 40 cm. Taking 2-year-old ginseng seedlings, disinfecting by sodium hypochlorite, transplanting into a planting pot paved with soil to be planted, keeping the plant spacing at 15cm, and conventionally culturing. And (4) observing the morbidity of the ginseng rust rot during harvesting, and calculating the disease index. The results are shown in Table 1.
The control effect of the treatment group of the present example 2 on the rust and rot was calculated by using the control group of the present example 1 as a control. The results are shown in Table 1.
The fresh weight of each individual plant of ginseng in the treatment group of the complex microbial inoculant of this example 2 was weighed. The results are shown in Table 2.
Comparative example 1
Taking 1kg of paecilomyces lilacinus microbial inoculum, wherein the viable count contained in the paecilomyces lilacinus microbial inoculum is 6 multiplied by 109cfu/g. Mixing the paecilomyces lilacinus microbial inoculum with 1 cubic meter of planting soil with the incidence index of ginseng rust rot more than 50% in the Changbai mountain ginseng planting base to obtain soil to be planted, and paving the soil to be planted in a planting pot with the thickness of 40 cm. Taking 2-year-old ginseng seedlings, disinfecting by sodium hypochlorite, transplanting into a planting pot paved with soil to be planted, keeping the plant spacing at 15cm, and conventionally culturing. And (4) observing the morbidity of the ginseng rust rot during harvesting, and calculating the disease index. The results are shown in Table 1.
The control group of example 1 was used as a control, and the control effect of the treatment group of comparative example 1 on rust and rot was calculated. The results are shown in Table 1.
The fresh weight of each individual plant of ginseng in the treatment group of the microbial agent of this comparative example 1 was weighed. The results are shown in Table 2.
Comparative example 2
1kg of bacillus amyloliquefaciens microbial inoculum is taken and evenly mixed to obtain the compound microbial inoculum. The number of viable bacteria contained in the bacillus amyloliquefaciens microbial inoculum is 6 multiplied by 1010cfu/g. Uniformly mixing the bacillus amyloliquefaciens microbial inoculum with 1 cubic meter of planting soil with the ginseng rust rot disease index more than 50 percent in the Changbai mountain ginseng planting base to obtain the soil to be planted. And spreading soil to be planted in the planting pot to be 40cm in thickness. Taking 2-year-old ginseng seedlings, disinfecting by sodium hypochlorite, transplanting into a planting pot paved with soil to be planted, keeping the plant spacing at 15cm, and conventionally culturing. And (4) observing the morbidity of the ginseng rust rot during harvesting, and calculating the disease index. The results are shown in Table 1.
The control group of example 1 was used as a control, and the control effect of the treatment group of comparative example 2 on rust and rot was calculated. The results are shown in Table 1.
The fresh weight of each individual plant of ginseng in the treatment group of the microbial agent of this comparative example 2 was weighed. The results are shown in Table 2.
Table 1 results of different treatment groups on incidence index and prevention and treatment effect of ginseng embroidery rot
| Treatment group | Index of disease condition | Controlling effect |
| Control group | 58.33 | -- |
| EXAMPLE 1 group | 7.34 | 85.19％ |
| EXAMPLE 2 group | 6.53 | 88.34％ |
| Comparative example 1 group | 27.89 | 46.71％ |
| Comparative example 2 group | 34.63 | 54.84％ |
Table 1 shows that after the compound microbial inoculum of the example 1 and the example 2 is treated, the disease index of the rust rot is obviously reduced, and the control effect is obviously higher than that of the microbial inoculum used alone. The compound microbial agent has the synergistic effect.
TABLE 2 comparison of fresh weight of individual plants of ginseng obtained in different treatment groups
| Treatment group | Fresh weight of single plant (g) |
| Control group | 98.7 |
| EXAMPLE 1 group | 111.3 |
| EXAMPLE 2 group | 106.6 |
| Comparison ofExample 1 group | 100.4 |
| Comparative example 2 group | 101.8 |
Table 2 shows that the fresh weight of each individual plant of ginseng after the treatment with the complex microbial inoculant in example 1 and example 2 is improved.
From the above embodiments, the invention provides a compound microbial agent and application thereof. When the paecilomyces lilacinus microbial inoculum and the bacillus amyloliquefaciens microbial inoculum are matched to prevent and treat the rust rot caused by the column spore fungi, the disease index is obviously reduced, the prevention and treatment effect is obviously enhanced, and the fresh weight of a single plant of the ginseng can be improved.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (8)
1. The compound microbial agent is characterized by comprising a paecilomyces lilacinus microbial agent and a bacillus amyloliquefaciens microbial agent;
the ratio of viable bacteria contained in the paecilomyces lilacinus microbial inoculum to viable bacteria contained in the bacillus amyloliquefaciens microbial inoculum is 1-5: 10 to 50.
2. The complex microbial inoculant according to claim 1, wherein the number of viable bacteria contained in the paecilomyces lilacinus inoculant is 2 x 109～1×1010cfu/g。
3. The complex microbial inoculant according to claim 2, wherein the number of viable bacteria contained in the bacillus amyloliquefaciens inoculant is 2 x 1010～1×1011cfu/g。
4. The use of the complex microbial inoculant of any one of claims 1 to 3 for controlling plant diseases caused by cylindrosporium.
5. The use of claim 4, wherein the plant comprises ginseng or American ginseng.
6. Use according to claim 5, wherein the plant disease comprises rust rot.
7. The use of claim 6, wherein the complex microbial inoculant is used by a clay treatment.
8. Use according to claim 7, characterized in that the clay-accompanying treatment is: 1-2 kg of compound microbial agent is added to each cubic meter of soil.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210073732.8A CN114231426A (en) | 2022-01-21 | 2022-01-21 | Compound microbial agent and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210073732.8A CN114231426A (en) | 2022-01-21 | 2022-01-21 | Compound microbial agent and application thereof |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103789234A (en) * | 2014-01-15 | 2014-05-14 | 吉林农业大学 | Bacillus amyloliquefaciens and application thereof |
| CN104498369A (en) * | 2014-12-05 | 2015-04-08 | 中国农业科学院植物保护研究所 | Trichoderma koningii and bacterial agent containing same and application thereof in prevention of cylindrocarpon destructans |
| CN109136144A (en) * | 2018-09-11 | 2019-01-04 | 通化医药高新区恩维泽生物科技有限公司 | A kind of ginseng special composite biological microbial inoculum and production preparation method |
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- 2022-01-21 CN CN202210073732.8A patent/CN114231426A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103789234A (en) * | 2014-01-15 | 2014-05-14 | 吉林农业大学 | Bacillus amyloliquefaciens and application thereof |
| CN104498369A (en) * | 2014-12-05 | 2015-04-08 | 中国农业科学院植物保护研究所 | Trichoderma koningii and bacterial agent containing same and application thereof in prevention of cylindrocarpon destructans |
| CN109136144A (en) * | 2018-09-11 | 2019-01-04 | 通化医药高新区恩维泽生物科技有限公司 | A kind of ginseng special composite biological microbial inoculum and production preparation method |
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