CN114223835A - Method for removing heavy metal in production process of microspheroidal algae and color protection method - Google Patents
Method for removing heavy metal in production process of microspheroidal algae and color protection method Download PDFInfo
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- 229910052793 cadmium Inorganic materials 0.000 claims abstract description 29
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- BDOYKFSQFYNPKF-UHFFFAOYSA-N 2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid;sodium Chemical compound [Na].[Na].OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O BDOYKFSQFYNPKF-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000003109 Disodium ethylene diamine tetraacetate Substances 0.000 claims description 38
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/27—Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption
- A23L5/273—Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption using adsorption or absorption agents, resins, synthetic polymers, or ion exchangers
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/40—Colouring or decolouring of foods
- A23L5/41—Retaining or modifying natural colour by use of additives, e.g. optical brighteners
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
The invention provides a method for removing heavy metals in a production process of pseudo-microsphere algae, which comprises the step of eluting pseudo-microsphere algae powder with an ethylene diamine tetraacetic acid disodium solution to obtain the pseudo-microsphere algae powder for removing the heavy metals. The invention uses ethylene diamine tetraacetic acid as a reagent to remove heavy metals in full-fat and degreased pseudo-microsphere algae powder, the method is simple and safe, and the obtained pseudo-microsphere algae can be suitable for food. Wherein the lead removal rate of the full-cream pseudo-microsphere algae powder is more than 70 percent, the cadmium removal rate is more than 90 percent, the lead removal rate of the degreased pseudo-microsphere algae powder is more than 85 percent, and the cadmium removal rate is more than 90 percent.
Description
Technical Field
The invention belongs to the technical field of algae treatment, and particularly relates to a method for removing heavy metals in a production process of microspheroidal algae and a color protection method.
Background
The Microsphaeroides is of genus Microsphaeroides of family Monomonas and genus Microsphaeroides. The microspherococcus sp is rapid in growth, small in cell particles and thick in cell wall, carbohydrate, protein and grease are rich in cells, polyunsaturated fatty acid (PUFA), especially eicosapentaenoic acid (EPA), is high in content, has the effects of reducing blood fat and preventing cardiovascular diseases, and becomes a research hotspot in the direction of preparing functional lipid in recent years. In 2021, 4 months, according to the regulations of food safety law, the safety evaluation materials of the new food raw materials of the pseudo-microsphere algae are examined and passed by the organization experts of the evaluation organization.
The full-fat pseudo-microsphere algae powder is a powdery product obtained by drying cultured microalgae, and contains abundant proteins (more than or equal to 45 percent), omega-3 substances, carotene or carotenoid and other trace elements and the like. The defatted microsphere algae powder is the residual matter of full-fat microsphere algae powder after the extraction process of microalgae oil, and contains rich protein (more than or equal to 50%), a small amount of omega-3 substances, carotene or carotenoid and other trace elements. Some heavy metal elements can be harmful to human body if eaten. Therefore, it is necessary to reduce or remove the heavy metal content in the pseudo-microspheroidal algae powder. However, in the prior art, the mixed solution of disodium ethylene diamine tetraacetate and citric acid is used for removing heavy metals in the algae mud, wherein the removal rates of lead and arsenic are respectively over 40% and 60%, and the removal rate is low.
In addition, in the process of culturing the microspheroidal algae, heavy metals in water can be enriched through physical adsorption, chemical adsorption and biological absorption, so that the heavy metal content in the microspheroidal algae is high, and the application of the microspheroidal algae in food is influenced. Furthermore, EDTA-2Na can change the color of the microspherococcus sp from green to yellow, thereby affecting the quality. There is a need to find a method for removing heavy metals from microspherococcus sp while maintaining the colour of the microspherococcus sp unchanged or inconspicuous.
Disclosure of Invention
In view of the above, the technical problem to be solved by the present invention is to provide a method for removing heavy metals in a production process of microspherococcus sp and a method for protecting color, wherein the method for removing heavy metals in a production process of microspherococcus sp provided by the present invention has a high removal rate of heavy metals, and can keep the color of microspherococcus sp unchanged or unchanged obviously.
The invention provides a method for removing heavy metals in a production process of pseudo-microsphere algae, which comprises the step of eluting pseudo-microsphere algae powder with an ethylene diamine tetraacetic acid disodium solution to obtain the pseudo-microsphere algae powder for removing the heavy metals.
Preferably, the method comprises the following steps:
mixing and stirring the pseudo-microsphere algae powder and an ethylene diamine tetraacetic acid solution for elution treatment to obtain an eluent;
and centrifuging the eluent, removing supernatant, and drying to obtain the pseudo-microsphere algae powder with the heavy metals removed.
Preferably, the microspheroidal algae powder is a powdery product obtained by drying cultured microspheroidal algae;
the pseudo-microsphere algae powder is full-fat pseudo-microsphere algae powder or degreased pseudo-microsphere algae powder.
Preferably, the concentration of the disodium ethylene diamine tetraacetate solution is 0.03-0.10 mol/L.
Preferably, the feed-liquid ratio of the pseudo-microsphere algae powder to the ethylene diamine tetraacetic acid solution is 1g: 10-20 ml.
Preferably, the temperature of the elution treatment is 25-33 ℃, and the time is 2-24 h.
Preferably, the heavy metals include lead and cadmium.
The invention also provides a method for protecting color in the process of removing heavy metal from the microspheroidal algae, which is to adjust the pH value of the microspheroidal algae powder to 7 in the process of eluting with the solution of disodium ethylene diamine tetraacetate to obtain the color-protected heavy metal-removed microspheroidal algae powder.
Preferably, the method comprises the following steps:
mixing the pseudo-microsphere algae powder with an ethylene diamine tetraacetic acid solution, adjusting the pH value to 6.5-7.5, and carrying out elution and color protection treatment to obtain an eluent;
and centrifuging the eluent, removing supernatant, and drying to obtain the color-protected heavy metal-removed pseudo-microsphere algae powder.
Preferably, the pH is adjusted with sodium hydroxide solution.
Compared with the prior art, the invention provides a method for removing heavy metals in the production process of pseudo-microsphere algae, which comprises the step of eluting pseudo-microsphere algae powder with an ethylene diamine tetraacetic acid disodium solution to obtain the pseudo-microsphere algae powder with the heavy metals removed. The invention uses ethylene diamine tetraacetic acid as a reagent to remove heavy metals in full-fat and degreased pseudo-microsphere algae powder, the method is simple and safe, and the obtained pseudo-microsphere algae can be suitable for food. Wherein the lead removal rate of the full-cream pseudo-microsphere algae powder is more than 70 percent, the cadmium removal rate is more than 90 percent, the lead removal rate of the degreased pseudo-microsphere algae powder is more than 85 percent, and the cadmium removal rate is more than 90 percent.
Detailed Description
The invention provides a method for removing heavy metals in a production process of pseudo-microsphere algae, which comprises the step of eluting pseudo-microsphere algae powder with an ethylene diamine tetraacetic acid disodium solution to obtain the pseudo-microsphere algae powder for removing the heavy metals.
Specifically, the method for removing heavy metals in the production process of the microspheroidal algae comprises the following steps:
mixing and stirring the pseudo-microsphere algae powder and an ethylene diamine tetraacetic acid solution for elution treatment to obtain an eluent;
and centrifuging the eluent, removing supernatant, and drying to obtain the pseudo-microsphere algae powder with the heavy metals removed.
Wherein the pseudo-microsphere algae powder is a powdery product obtained by drying cultured pseudo-microsphere algae; wherein the water content of the pseudo-microsphere algae powder is less than or equal to 6 percent. The drying method is not particularly limited, and the drying method known to those skilled in the art can be used. In the present invention, the drying is preferably carried out by a spray drying method.
The pseudo-microsphere algae powder is full-fat pseudo-microsphere algae powder or degreased pseudo-microsphere algae powder.
The full-fat microsphere algae powder is a powdery product obtained by drying cultured microsphere algae, and contains abundant proteins (more than or equal to 45 percent), omega-3 substances, carotene or carotenoid and other trace elements and the like. The defatted microsphere algae powder is the residual matter of full-fat microsphere algae powder after microsphere algae oil extraction process, and contains rich protein (not less than 50%), a small amount of omega-3 matter, carotene or carotenoid and other trace elements.
The concentration of the disodium ethylene diamine tetraacetate solution is 0.03-0.10 mol/L, and is preferably any value between 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.10 or 0.03-0.10 mol/L.
The feed-liquid ratio of the microspheroidal algae powder to the ethylene diamine tetraacetic acid disodium solution is 1g to 10-20 ml, preferably 1g to 10ml, 1g to 11ml, 1g to 12ml, 1g to 13ml, 1g to 14ml, 1g to 15ml, 1g to 16ml, 1g to 17ml, 1g to 18ml, 1g to 19ml, 1g to 20ml, or any value between 1g to 10-20 ml.
Mixing and stirring the pseudo-microsphere algae powder and an ethylene diamine tetraacetic acid solution for elution treatment, wherein the temperature of the elution treatment is 25-33 ℃, and the preferred temperature is normal temperature; the time is 2-24 h, preferably any value between 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, or 2-24 h.
Wherein the stirring speed is 400-800 r/min, preferably 400, 500, 600, 700, 800 or any value between 400-800 r/min.
After elution treatment, obtaining eluent; and centrifuging the eluent, removing supernatant, washing the lower precipitate with water, and drying to obtain the pseudo-microsphere algae powder with the heavy metals removed. The centrifugal speed is 4000r/min, the centrifugal time is 15-30 min, and the preferable value is 15, 20, 25, 30 or any value between 15-30 min. The present invention is not limited to any particular manner of drying, including but not limited to freeze drying, spray drying, oven drying or air drying.
In the present invention, the heavy metals include lead and cadmium.
The invention also provides a method for protecting color in the process of removing heavy metal from the microspheroidal algae, which is to adjust the pH value of the microspheroidal algae powder to 7 in the process of eluting with the solution of disodium ethylene diamine tetraacetate to obtain the color-protected heavy metal-removed microspheroidal algae powder.
Specifically, the method for protecting the color in the process of removing the heavy metal from the microspheroidal algae comprises the following steps:
mixing the pseudo-microsphere algae powder with an ethylene diamine tetraacetic acid solution, adjusting the pH value to 7, and carrying out elution and color protection treatment to obtain an eluent;
and centrifuging the eluent, removing supernatant, and drying to obtain the color-protected heavy metal-removed pseudo-microsphere algae powder.
Wherein the pseudo-microsphere algae powder is a powdery product obtained by drying cultured pseudo-microsphere algae; wherein the water content of the pseudo-microsphere algae powder is less than or equal to 6 percent.
The pseudo-microsphere algae powder is full-fat pseudo-microsphere algae powder or degreased pseudo-microsphere algae powder.
The concentration of the disodium ethylene diamine tetraacetate solution is 0.03-0.10 mol/L, and is preferably any value between 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.10 or 0.03-0.10 mol/L.
The feed-liquid ratio of the microspheroidal algae powder to the ethylene diamine tetraacetic acid disodium solution is 1g to 10-20 ml, preferably 1g to 10ml, 1g to 11ml, 1g to 12ml, 1g to 13ml, 1g to 14ml, 1g to 15ml, 1g to 16ml, 1g to 17ml, 1g to 18ml, 1g to 19ml, 1g to 20ml, or any value between 1g to 10-20 ml.
Mixing the pseudo-microsphere algae powder with an ethylene diamine tetraacetic acid solution, adjusting the pH value to 7, and carrying out elution and color protection treatment.
Among them, the pH is preferably adjusted with a sodium hydroxide solution. The temperature of the elution and color protection treatment is 25-33 ℃, and the preferred temperature is normal temperature; the time is 2-24 h, preferably any value between 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, or 2-24 h.
Stirring is carried out in the elution color protection treatment process, and the stirring rotating speed is 400-800 r/min, preferably 400, 500, 600, 700, 800 or any value between 400-800 r/min.
After elution treatment, obtaining eluent; and centrifuging the eluent, removing supernatant, washing the lower precipitate with water, and drying to obtain the color-protected pseudo-microsphere algae powder with the heavy metals removed. The centrifugal speed is 4000r/min, the centrifugal time is 15-30 min, and the preferable value is 15, 20, 25, 30 or any value between 15-30 min. The present invention is not limited to any particular manner of drying, including but not limited to freeze drying, spray drying, oven drying or air drying.
The invention uses ethylene diamine tetraacetic acid as a reagent to remove heavy metals in full-fat and degreased pseudo-microsphere algae powder, the method is simple and safe, and the obtained pseudo-microsphere algae can be suitable for food. Wherein the lead removal rate of the full-cream pseudo-microsphere algae powder is more than 70 percent, the cadmium removal rate is more than 90 percent, the lead removal rate of the degreased pseudo-microsphere algae powder is more than 85 percent, and the cadmium removal rate is more than 90 percent.
For further understanding of the present invention, the following examples are provided to illustrate the method for removing heavy metals and protecting color in the production process of microspherococcus sp.
Example 1:
weighing 33.6g of disodium ethylene diamine tetraacetate, placing the disodium ethylene diamine tetraacetate into a clean beaker, adding 1L of distilled water into the beaker to fully dissolve the disodium ethylene diamine tetraacetate to obtain 0.1mol/L disodium ethylene diamine tetraacetate solution, adding 20g of full-fat pseudo-microsphere algae powder into the clean beaker, adding the prepared disodium ethylene diamine tetraacetate solution according to the material-liquid ratio of 1g to 15ml, adjusting the pH value to be neutral by using sodium hydroxide solution, stirring for 6 hours at normal temperature, centrifuging for 20 minutes at 4000r/min, removing supernatant, and washing for 1 time by using water. And (5) freezing and drying the precipitate to obtain a target product.
Sample detection: taking 3 groups of the target products obtained in the example 1 as experimental group samples, and detecting and measuring the lead and cadmium contents (the untreated full-cream microspheroidal algae powder has the lead content of 3.57mg/kg and the cadmium content of 1.80 mg/kg).
The detection results are as follows: the content of lead in the experimental group sample is 0.95mg/kg, and the content of cadmium in the experimental group sample is 0.15mg/kg, so that the content of lead and cadmium in the experimental group sample treated by the eluent is greatly reduced, and the removal rates respectively reach 73.4% and 91.7%.
Example 2:
weighing 33.6g of disodium ethylene diamine tetraacetate, placing the disodium ethylene diamine tetraacetate into a clean beaker, adding 1L of distilled water into the beaker to fully dissolve the disodium ethylene diamine tetraacetate to obtain 0.1mol/L disodium ethylene diamine tetraacetate solution, taking the clean beaker, adding 20g of degreased pseudo-microsphere algae powder into the clean beaker, adding the prepared disodium ethylene diamine tetraacetate solution according to the material-to-liquid ratio of 1g to 10ml, stirring the mixture for 24 hours at normal temperature, centrifuging the mixture for 20 minutes at 4000r/min, removing supernatant, and washing the mixture for 1 time. And (5) freezing and drying the precipitate to obtain a target product.
Sample detection: taking 3 groups of the target products obtained in the example 2 as experimental group samples, and detecting and determining the lead and cadmium contents (the untreated degreased microspheroidal algae powder has the lead content of 11.74mg/kg and the cadmium content of 3.1 mg/kg).
The detection results are as follows: the content of lead in the experimental group sample is 0.97mg/kg, and the content of cadmium in the experimental group sample is 0.048mg/kg, so that the content of lead and cadmium in the experimental group sample treated by the eluent is greatly reduced, and the removal rates respectively reach 91.7% and 98.4%.
Comparative example 1
Removing heavy metals in the microspherical algae slurry: adding 70ml of pure water and 9.045g of disodium ethylene diamine tetraacetate into a beaker to prepare a solution, adding 200ml of concentrated full-fat algae slurry (the concentration is 120g of dry algae powder/L of algae slurry), adjusting the pH value to be neutral by using a sodium hydroxide solution, stirring at normal temperature, stirring for 6 hours, centrifuging at 4000r/min for 15 minutes, removing supernatant, washing for 1 time by using water according to the material-liquid ratio of 1:5, precipitating, freezing and drying to obtain the target product.
Sample detection: taking 3 groups of the target products obtained in the example 1 as experimental group samples, taking 3 groups of the target products obtained in the comparative example 1 as control group samples, and detecting the experimental group samples and the control group samples to determine the lead content (the lead content of the untreated original microspherococcus powder in the example 1 is 3.57mg/kg, and the lead content of the untreated concentrated algae slurry in the comparative example 1 is 6.50mg/kg after freeze-drying).
The detection results are as follows: the content of lead in the experimental group sample is 0.95mg/kg, and the content of lead in the control group sample is 4.60mg/kg, so that the content of lead in the experimental group sample treated by the eluent is greatly reduced, the removal rate reaches 73.4%, and the reduction range of the content of lead in the control group sample is small and is only reduced by 29.2%.
Example 3
Weighing 33.6g of disodium ethylene diamine tetraacetate, placing the disodium ethylene diamine tetraacetate into a clean beaker, adding 1L of distilled water into the beaker to fully dissolve the disodium ethylene diamine tetraacetate to obtain 0.1mol/L disodium ethylene diamine tetraacetate solution, taking the clean beaker, adding 20g of pseudo-microsphere algae powder into the clean beaker, adding the prepared disodium ethylene diamine tetraacetate solution according to the material-to-liquid ratio of 1g to 15ml, adjusting the pH to be neutral by using a sodium hydroxide solution, stirring for 24 hours at normal temperature, centrifuging for 20 minutes at 4000r/min, removing a supernatant, and repeating the operation once. And (5) freezing and drying the precipitate to obtain a target product.
Comparative example 2
Comparative example 2 the pH was adjusted without using NaOH solution, and the other operations were the same as in example 3
Sample detection: taking 3 groups of the target products obtained in the example 3 as experimental group samples, taking 3 groups of the target products obtained in the comparative example 1 as control group samples, and detecting the experimental group samples and the control group samples to determine the lead content (the untreated original microspheroidal algae powder lead content is 3.57 mg/kg).
The detection results are as follows: the content of lead in the experimental group sample is 0.65mg/kg, and the content of lead in the control group sample is 0.69mg/kg, so that the content of lead in the experimental group sample and the content of lead in the control group sample after the treatment by the eluent are both greatly reduced, and the removal rates respectively reach 81.8% and 80.7%. The color of the experimental group sample is green and is closer to the color of the untreated pseudo-microsphere algae powder than the color of the control group sample by comparing the colors of the two samples.
Example 4
Weighing 33.6g of disodium ethylene diamine tetraacetate, placing the disodium ethylene diamine tetraacetate into a clean beaker, adding 1L of distilled water into the beaker to fully dissolve the disodium ethylene diamine tetraacetate to obtain 0.1mol/L disodium ethylene diamine tetraacetate solution, adding 20g of full-fat pseudo-microsphere algae powder into the clean beaker, adding the prepared disodium ethylene diamine tetraacetate solution according to the material-to-liquid ratio of 1g to 10ml, adjusting the pH value to be neutral by using sodium hydroxide solution, stirring for 6 hours at normal temperature, centrifuging for 20 minutes at 4000r/min, removing supernatant, and washing for 1 time by using water. And (5) freezing and drying the precipitate to obtain a target product.
Sample detection: taking 3 groups of the target products obtained in the example 4 as experimental group samples, and detecting and measuring the lead and cadmium contents (the untreated full-cream microspheroidal algae powder has the lead content of 3.57mg/kg and the cadmium content of 1.80 mg/kg).
The detection results are as follows: the content of lead in the experimental group sample is 1.05mg/kg, and the content of cadmium in the experimental group sample is 0.43mg/kg, so that the content of lead and cadmium in the experimental group sample treated by the eluent is greatly reduced, and the removal rates respectively reach 70.6% and 76.1%.
Example 5
Weighing 13.44g of disodium ethylene diamine tetraacetate, placing the disodium ethylene diamine tetraacetate into a clean beaker, adding 1L of distilled water into the beaker to fully dissolve the disodium ethylene diamine tetraacetate to obtain 0.04mol/L disodium ethylene diamine tetraacetate solution, taking the clean beaker, adding 20g of full-fat pseudo-microsphere algae powder into the clean beaker, adding the prepared disodium ethylene diamine tetraacetate solution according to the material-liquid ratio of 1g to 15ml, stirring for 8 hours at normal temperature, centrifuging for 20 minutes at 4000r/min, removing supernatant, and washing for 1 time. And (5) freezing and drying the precipitate to obtain a target product.
Sample detection: taking 3 groups of the target products obtained in the example 5 as experimental group samples, and detecting and measuring the lead and cadmium contents (the untreated full-cream microspheroidal algae powder has the lead content of 3.57mg/kg and the cadmium content of 1.80 mg/kg).
The detection results are as follows: the content of lead in the experimental group sample is 1.01mg/kg, and the content of cadmium in the experimental group sample is 0.31mg/kg, so that the content of lead and cadmium in the experimental group sample treated by the eluent is greatly reduced, and the removal rates respectively reach 71.7% and 82.8%.
Example 6
Weighing 33.6g of disodium ethylene diamine tetraacetate, placing the disodium ethylene diamine tetraacetate into a clean beaker, adding 1L of distilled water into the beaker to fully dissolve the disodium ethylene diamine tetraacetate to obtain 0.1mol/L disodium ethylene diamine tetraacetate solution, taking the clean beaker, adding 20g of full-fat pseudo-microsphere algae powder into the clean beaker, adding the prepared disodium ethylene diamine tetraacetate solution according to the material-liquid ratio of 1g to 15ml, stirring the mixture at normal temperature for 6 hours, centrifuging the mixture for 20 minutes at 4000r/min, removing supernatant, and washing the mixture for 1 time. And (5) freezing and drying the precipitate to obtain a target product.
Sample detection: taking 3 groups of the target products obtained in the example 6 as experimental group samples, and detecting and measuring the lead and cadmium contents (the untreated full-cream microspheroidal algae powder has the lead content of 3.57mg/kg and the cadmium content of 1.80 mg/kg).
The detection results are as follows: the content of lead in the experimental group sample is 0.92mg/kg, and the content of cadmium in the experimental group sample is 0.17mg/kg, so that the content of lead and cadmium in the experimental group sample treated by the eluent is greatly reduced, and the removal rates respectively reach 74.2% and 90.5%.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (10)
1. A method for removing heavy metals in a production process of pseudo-microsphere algae is characterized in that the pseudo-microsphere algae powder is eluted by an ethylene diamine tetraacetic acid disodium solution to obtain the pseudo-microsphere algae powder with the heavy metals removed.
2. The method of claim 1, comprising the steps of:
mixing and stirring the pseudo-microsphere algae powder and an ethylene diamine tetraacetic acid solution for elution treatment to obtain an eluent;
and centrifuging the eluent, removing supernatant, and drying to obtain the pseudo-microsphere algae powder with the heavy metals removed.
3. The method according to claim 1 or 2, wherein the powder of the Nannochloropsis is a powder product obtained by drying cultured Nannochloropsis;
the pseudo-microsphere algae powder is full-fat pseudo-microsphere algae powder or degreased pseudo-microsphere algae powder.
4. The method as claimed in claim 1 or 2, wherein the concentration of the disodium edetate solution is 0.03-0.10 mol/L.
5. The method as claimed in claim 1 or 2, wherein the ratio of the microspheroidal algae powder to the disodium ethylene diamine tetraacetate solution is 1g: 10-20 ml.
6. The method according to claim 1 or 2, wherein the temperature of the elution treatment is 25 to 33 ℃ and the time is 2 to 24 hours.
7. The method of claim 1 or 2, wherein the heavy metals include lead and cadmium.
8. A method for protecting color in a process of removing heavy metals from microspheroidal algae is characterized in that the pH value of microspheroidal algae powder is adjusted to 7 in the process of elution treatment by using an ethylene diamine tetraacetic acid disodium solution, so that the color-protected heavy metal-removed microspheroidal algae powder is obtained.
9. The method of claim 8, comprising the steps of:
mixing the pseudo-microsphere algae powder with an ethylene diamine tetraacetic acid solution, adjusting the pH value to 6.5-7.5, and carrying out elution and color protection treatment to obtain an eluent;
and centrifuging the eluent, removing supernatant, and drying to obtain the color-protected heavy metal-removed pseudo-microsphere algae powder.
10. The method according to claim 8 or 9, characterized in that the pH is adjusted with sodium hydroxide solution.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102492626A (en) * | 2011-12-16 | 2012-06-13 | 新奥科技发展有限公司 | Nannochloropsis sp and application thereof |
| CN103798122A (en) * | 2014-03-05 | 2014-05-21 | 宁波大学 | Method for reducing content of heavy metals in spiral seaweed |
| CN106190852A (en) * | 2016-08-31 | 2016-12-07 | 天津海友佳音生物科技股份有限公司 | A kind of remove the method for heavy metal in algae |
| CN107118740A (en) * | 2017-05-03 | 2017-09-01 | 闽南师范大学 | A kind of compound eluted composition for being used to remove heavy metal in micro- algae powder |
| CN110179047A (en) * | 2019-07-10 | 2019-08-30 | 中盐工程技术研究院有限公司 | The minimizing technology of heavy metal in a kind of algal gel |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102492626A (en) * | 2011-12-16 | 2012-06-13 | 新奥科技发展有限公司 | Nannochloropsis sp and application thereof |
| CN103798122A (en) * | 2014-03-05 | 2014-05-21 | 宁波大学 | Method for reducing content of heavy metals in spiral seaweed |
| CN106190852A (en) * | 2016-08-31 | 2016-12-07 | 天津海友佳音生物科技股份有限公司 | A kind of remove the method for heavy metal in algae |
| CN107118740A (en) * | 2017-05-03 | 2017-09-01 | 闽南师范大学 | A kind of compound eluted composition for being used to remove heavy metal in micro- algae powder |
| CN110179047A (en) * | 2019-07-10 | 2019-08-30 | 中盐工程技术研究院有限公司 | The minimizing technology of heavy metal in a kind of algal gel |
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