CN114223658B - Method for preventing and treating spodoptera frugiperda by using sex pheromone disorientation agent and application - Google Patents
Method for preventing and treating spodoptera frugiperda by using sex pheromone disorientation agent and application Download PDFInfo
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- CN114223658B CN114223658B CN202111570192.6A CN202111570192A CN114223658B CN 114223658 B CN114223658 B CN 114223658B CN 202111570192 A CN202111570192 A CN 202111570192A CN 114223658 B CN114223658 B CN 114223658B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
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Abstract
The invention discloses a method for preventing spodoptera frugiperda by using a sex pheromone disorienting agent, belonging to the field of biological prevention and control. The invention utilizes the artificially synthesized sex pheromone disorienting agent component cis-8-tetradecene acetate to prevent and control Spodoptera frugiperda by interfering the information transmission between Spodoptera frugiperda male and female moths. The spodoptera frugiperda disorienting agent can effectively reduce the mating rate between males and females, and generally can reduce the mating rate by more than 85%. Meanwhile, the composition has long lasting period and strong specificity, is environment-friendly and has important significance for the comprehensive treatment of spodoptera frugiperda.
Description
Technical Field
The invention belongs to the technical field of biological control, and particularly relates to a sex pheromone disorientation agent for controlling spodoptera frugiperda and application thereof.
Background
Spodoptera frugiperda, of the Lepidoptera (Lepidotera) family of the Noctuidae, native to tropical and subtropical regions of the America. The data report that Spodoptera frugiperda is a multi-feeding pest, can harm 353 plants in total of 76 families such as Gramineae, Solanaceae, Brassicaceae, Caryophyllaceae and Compositae, and especially likes gramineae plants such as corn, sorghum, rice, wheat and the like. Spodoptera frugiperda is a pest in Africa since 2016, and now has become a major pest in Africa, America, oceania and Asia, and is recognized by the food and agriculture organization of the United nations as a globally important agricultural migratory pest. The 1 month in 2019 is imported from the border of Mian into China, the border has spread to 129 counties in the whole province to the end of 6 months, the outbreak period is mainly concentrated in summer, and the outbreak area is 55.62 ten thousand hm266.59 ten thousand hm of total annual area283.52% of the total weight of the steel. The Spodoptera frugiperda is divided into a corn type and a rice type according to the preference of taking food, wherein the corn type is mainly harmful to crops such as corn, wheat and the like, and the rice type is mainly harmful to crops such as rice, large crabgrass and the like.
In the comprehensive treatment of plant diseases and insect pests, chemical prevention and control are always one of the main means. However, the prevention and control effect is obviously reduced along with the generation of the drug resistance of pests, and meanwhile, the application of chemical pesticides can cause a series of problems of influencing the survival of natural enemies, causing ecological environment damage, ensuring food quality safety and the like, thereby becoming an important obstacle for the sustainable development of agriculture. Research reports that with the wide use of chemical insecticides, the tolerance of spodoptera frugiperda to chemical insecticides such as organophosphates, carbamates and pyrethroids is obviously enhanced, and the phenomenon of resistance to insecticides is increasingly prominent. The insect sex pheromone has the advantages of high efficiency, low toxicity, no pollution, no damage to beneficial insects and the like, plays an increasingly important role in the Integrated Pest Management (IPM) of pests, and has good economic, social and ecological benefits. At present, monitoring the occurrence of pests by using insect sex pheromones as an attraction source is accepted by more and more researchers, but sex pheromone disorienting agents are considered to be the most efficient method in controlling pests. The sex pheromone disorientation agent has the principle of interfering the mating communication among the male and female worms, reducing the mating rate of the adults to the maximum extent, reducing the number of reproduction, playing the dual role of interference and trapping so as to achieve the aim of insect prevention, and is an efficient, green and environment-friendly prevention and treatment method.
Disclosure of Invention
The invention aims to provide a sex pheromone disorienting agent for preventing and treating spodoptera frugiperda.
A sex pheromone disorienting agent for preventing and treating Spodoptera frugiperda comprises cis-8-tetradecene acetate as main ingredient. Preferably, the dosage of cis-8-tetradecene acetate is 5-15 mg;
a sex pheromone disorienting agent for preventing and treating Spodoptera frugiperda is disclosed, wherein the number of the disorienting agent lure core is preferably 500-800 per hectare.
The invention also provides a preparation method of the spodoptera frugiperda sex pheromone disorienting agent. Preparing mother liquor with different concentrations from cis-8-tetradecene acetate by using corn oil as solvent, adding into polyvinyl chloride capillary (PVC) (length 20cm × inner diameter 1.5mm) at a dosage of 5mg-15mg, heating and sealing two ends of the PVC capillary after adding, and making into sex pheromone attractant core of Spodoptera frugiperda.
The invention has the beneficial effects that: 1. is environment-friendly and has no toxicity or harm to human bodies; 2. the raw materials are cheap and easy to obtain, and the economic cost is low; 3. the operation is simple, and the use is convenient; 4. the stability is good, the persistent period is long, the Spodoptera frugiperda does not have drug resistance, and the method has important significance for comprehensively treating the Spodoptera frugiperda.
Drawings
FIG. 1 is a schematic diagram of the chemical structure of cis-8-tetradecene acetate.
FIG. 2 Release Rate of cis-8-tetradecene acetate.
Detailed Description
The following detailed description of the present invention is provided in conjunction with the accompanying drawings, but it should be understood that the scope of the present invention is not limited to the specific embodiments.
Example 1 preparation of Spodoptera frugiperda sex pheromone disorienting agent
Polyvinyl chloride capillary (PVC) (length 20 cm. times. inner diameter 1.5mm) with slow release effect is selected as carrier. Injecting the corn oil solution containing cis-8-tetradecene acetate into a polyvinyl chloride capillary tube at a dosage of 5-15mg, adding 2% of 2, 6-di-tert-butyl-4-methylphenol (BHT) as a stabilizer, heating and sealing two ends of the capillary tube after finishing the addition, and preparing the spodoptera frugiperda sex pheromone attractant core. Before the test, the lure is placed in a refrigerator with the temperature of-20 ℃ and is stored for standby.
Example 2 field bioactivity assay
The lure prepared in example 1 was selected and hung in a field of corn with severe spodoptera frugiperda damage, and the trap was a barrel-type trap selected and located between 20-30cm above the crop. Each of the above-described lure treatments was set to 3 replicates. The different doses, number of placements and mating inhibition rates are shown in table 1.
The test result shows that cis-8-tetradecene acetate has different degrees of inhibitory action on mating of spodoptera frugiperda, the inhibition on mating is more obvious along with the increase of the dosage, and when the dosage exceeds 11mg and the set number per hectare is 500, the inhibition on mating rate is more than 90%. Meanwhile, when the dose of cis-8-tetradecene acetate was 5mg, the mating inhibition rate was higher as the number per hectare set was increased. In view of cost, a cis-8-tetradecene acetate content of 5mg and a set number of 500 per hectare is suggested.
Example 3 Spodoptera frugiperda sex pheromone disorienting agent field Release Rate
The lure prepared in example 1 (cis-8-tetradecene acetate 5mg) was selected, hung in the field, and the cis-8-tetradecene acetate remaining in the lure was extracted every 15 days for 4 months, and quantitatively determined by gas chromatography. After 4 months, the content of cis-8-tetradecene acetate is still 1.54mg (figure 2), which shows that the spodoptera frugiperda sex pheromone disorienting agent has the field lasting period of more than 4 months and can be used for preventing and controlling the spodoptera frugiperda.
The sex pheromone disorientation agent can interfere the mating communication between male and female spodoptera frugiperda, reduces the mating rate of adults to the maximum extent, reduces the number of breeding, plays a role in interference and trapping, is an efficient, green and environment-friendly control method, and has important significance for comprehensive control of spodoptera frugiperda.
The above disclosure is only for the specific embodiment of the present invention, but the present invention is not limited thereto, and any variations that can be considered by those skilled in the art should fall within the scope of the present invention.
Claims (2)
1. The sex pheromone is cis-8-tetradecene acetate, the cis-8-tetradecene acetate is added into polyvinyl chloride capillaries with the length of 20cm multiplied by the inner diameter of 1.5mm and the dosage is 5-15mg, after the addition is finished, the two ends of the capillaries are heated and sealed, and the sex pheromone attractant core of the sex pheromone spodoptera frugiperda spodoptera is prepared.
2. The use of the sex pheromone attractant of claim 1 for controlling spodoptera frugiperda, wherein the attractant core is fixed 20-30cm above the crop, and 500 attractant cores per hectare are of the sex pheromone attractant core are provided.
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| CN111602659B (en) * | 2020-06-29 | 2021-03-16 | 广东省科学院生物工程研究所 | Sex attractant for Spodoptera frugiperda, and preparation method and application thereof |
| CN111713497A (en) * | 2020-07-21 | 2020-09-29 | 中国农业科学院植物保护研究所 | Efficient attractant for spodoptera frugiperda and application of efficient attractant |
| CN113142207B (en) * | 2021-05-07 | 2023-05-23 | 中国农业科学院植物保护研究所 | Noctuid disorientation agent and application thereof |
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Effective date of registration: 20221222 Address after: No.985-7, Fenghuangshan Road, Huancui District, Weihai City, Shandong Province 264200 Patentee after: Xinyuan fruit industry (Shandong) Group Co.,Ltd. Address before: 100193 Institute of plant protection, Chinese Academy of Agricultural Sciences, 2 West Old Summer Palace Road, Beijing, Haidian District Patentee before: INSTITUTE OF PLANT PROTECTION CHINESE ACADEMY OF AGRICULTURAL SCIENCES |
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Denomination of invention: A method and application of using sex pheromone pheromone to control Spodoptera frugivora Effective date of registration: 20230328 Granted publication date: 20220722 Pledgee: SHANDONG WEIHAI RURAL COMMERCIAL BANK Co.,Ltd. Pledgor: Xinyuan fruit industry (Shandong) Group Co.,Ltd. Registration number: Y2023980036281 |
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