CN114223424A - Chemical treatment method for inhibiting anaerobic respiration of mature litchi fruits and delaying sugar drop - Google Patents
Chemical treatment method for inhibiting anaerobic respiration of mature litchi fruits and delaying sugar drop Download PDFInfo
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- CN114223424A CN114223424A CN202111512234.0A CN202111512234A CN114223424A CN 114223424 A CN114223424 A CN 114223424A CN 202111512234 A CN202111512234 A CN 202111512234A CN 114223424 A CN114223424 A CN 114223424A
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- litchi
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- anaerobic respiration
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- 235000013399 edible fruits Nutrition 0.000 title claims abstract description 28
- 238000011282 treatment Methods 0.000 title claims abstract description 21
- 230000004099 anaerobic respiration Effects 0.000 title claims abstract description 17
- 238000000034 method Methods 0.000 title claims abstract description 13
- 239000000126 substance Substances 0.000 title claims abstract description 10
- 230000002401 inhibitory effect Effects 0.000 title claims abstract description 9
- 241001629511 Litchi Species 0.000 title claims abstract 8
- GPXLRLUVLMHHIK-UHFFFAOYSA-N forchlorfenuron Chemical compound C1=NC(Cl)=CC(NC(=O)NC=2C=CC=CC=2)=C1 GPXLRLUVLMHHIK-UHFFFAOYSA-N 0.000 claims abstract description 24
- 239000005979 Forchlorfenuron Substances 0.000 claims abstract description 12
- 238000005507 spraying Methods 0.000 claims abstract description 4
- 230000003111 delayed effect Effects 0.000 abstract description 3
- 238000012271 agricultural production Methods 0.000 abstract description 2
- 230000006866 deterioration Effects 0.000 abstract description 2
- 239000000796 flavoring agent Substances 0.000 abstract description 2
- 235000019634 flavors Nutrition 0.000 abstract description 2
- 230000014759 maintenance of location Effects 0.000 abstract description 2
- 230000002035 prolonged effect Effects 0.000 abstract description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 18
- 244000183278 Nephelium litchi Species 0.000 description 16
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 15
- 230000000694 effects Effects 0.000 description 13
- 108010051210 beta-Fructofuranosidase Proteins 0.000 description 9
- 235000011073 invertase Nutrition 0.000 description 8
- 230000014509 gene expression Effects 0.000 description 7
- 108090000790 Enzymes Proteins 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 6
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 6
- 229930006000 Sucrose Natural products 0.000 description 6
- 239000005720 sucrose Substances 0.000 description 6
- 102000007698 Alcohol dehydrogenase Human genes 0.000 description 5
- 108010021809 Alcohol dehydrogenase Proteins 0.000 description 5
- 239000001573 invertase Substances 0.000 description 5
- 230000007935 neutral effect Effects 0.000 description 4
- 238000009825 accumulation Methods 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 229930091371 Fructose Natural products 0.000 description 2
- 239000005715 Fructose Substances 0.000 description 2
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 108010011939 Pyruvate Decarboxylase Proteins 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- 235000015742 Nephelium litchi Nutrition 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000003851 biochemical process Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000001925 catabolic effect Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 239000003640 drug residue Substances 0.000 description 1
- 210000005069 ears Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 230000005070 ripening Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- Life Sciences & Earth Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Cultivation Of Plants (AREA)
- Saccharide Compounds (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a chemical treatment method for inhibiting anaerobic respiration of mature litchi fruits and delaying sugar drop, and particularly relates to the technical field of agricultural production. The method comprises the following processing steps: spraying forchlorfenuron (CPPU) on the fruit cluster of the Feizixiao three weeks before the Feizixiao litchi is picked. By adopting the technical scheme of the invention, the problems of rapid sugar drop and flavor loss after litchi ripens are solved, the deterioration of fruit quality is delayed, and the tree retention time after litchi ripens is prolonged.
Description
Technical Field
The invention relates to the technical field of agricultural production, in particular to a chemical treatment method for inhibiting anaerobic respiration of mature litchi fruits and delaying sugar loss.
Background
China is the first big country of litchi production, but the main cultivars in the main production area are single and centralized in marketing, and meanwhile, litchi is extremely easy to rot, and the retention period of mature fruits is short, so that great pressure is brought to the sales generation. The selling pressure can be relieved to a certain extent through refrigeration and preservation; however, litchi fruits need to be harvested as soon as possible once they are ripe, because the quality of the fruits left in the tree also deteriorates rapidly, and peculiar smell is generated due to the decrease of sugar content and accumulation of anaerobic respiration products, so that the litchi fruits lose commodity value. If the physiological and biochemical processes with deteriorated quality can be delayed by adopting a regulation and control technology, the tree remaining time can be prolonged, the buffer time is won for the harvest, and the sales pressure and the labor intensity of the harvest are reduced.
Disclosure of Invention
The invention aims to provide a chemical treatment method for inhibiting anaerobic respiration of mature litchi fruits and delaying sugar drop, and solves the problems of rapid sugar drop and flavor loss after mature litchi fruits.
In order to achieve the purpose, the technical scheme of the invention is as follows: a chemical treatment method for inhibiting the anaerobic respiration of mature litchi fruits and delaying sugar loss comprises the following treatment steps: spraying forchlorfenuron (CPPU) on the fruit cluster of the Feizixiao three weeks before the Feizixiao litchi is picked.
The principle of the technical scheme is as follows: the component change of the harvested fruit trees of the Feizixiao trees with the mature consequence meat is delayed, the decrease of the sucrose of the fruits is obvious, the decrease of the reducing sugar is slow, and the total sugar is obviously decreased along with the decrease of the sucrose; meanwhile, anaerobic respiration is started by the pulp cells, so that the contents of pulp ethanol and acetic acid are obviously improved, and the peculiar smell of the fruits is generated. Sucrose reduction is associated with high soluble invertase activity and gene expression, while ethanol and acetate content is closely associated with pyruvate decarboxylase and alcohol dehydrogenase activity and gene expression.
Further, the concentration of the forchlorfenuron (CPPU) is 10-20 mg/L.
Compared with the prior art, the beneficial effect of this scheme:
the scheme solves the problem of rapid quality deterioration when the litchi is ripe and left on a tree. Besides improving the structure of the peel and the storage stability of the fruit, the method can also delay the speed of sugar removal of the pulp of the mature fruit of the tree, inhibit the generation of anaerobic respiration and the accumulation of products, prolong the tree-leaving period by more than 10 days, and simultaneously ensure that the drug residue is lower than the upper safety limit of European and American countries.
Drawings
FIG. 1 is a graph of the change in the brix of the two-week-old effect of the mature Feizixiao litchi trees treated in examples 1 to 5 at different concentrations;
FIG. 2 is a graph of the change in the levels of sucrose, glucose, fructose, total sugar and the anaerobic respiration products ethanol and acetic acid in the pulp of Feizixiao mature fruit trees at 2 weeks and 3 weeks after treatment in example 1;
FIG. 3 is a graph showing the changes in gene expression and enzyme activity of soluble acid invertase and neutral invertase at 2 weeks and 3 weeks of a Feizixiao mature fruit tree after the treatment of example 1;
FIG. 4 is a graph showing the changes in the expression and enzyme activity of the pulp anaerobic respiratory enzymes PDC and ADH in 2 weeks and 3 weeks after the treatment of Feizixiao mature fruit tree in example 1.
Detailed Description
The present invention will be described in further detail below by way of specific embodiments:
example 1
A chemical treatment method for inhibiting the anaerobic respiration of mature litchi fruits and delaying sugar loss comprises the following treatment steps: spraying forchlorfenuron (CPPU) with the concentration of 20mg/L to the dropping liquid 50 days after the Feizixiao litchi blooms (namely, the pulp is expanded to the first stage and about three weeks before picking). The changes in the residual endocarp and pulp concentrations at two and four weeks after the above feizixiao treatment with forchlorfenuron (CPPU) are shown in table 1 below.
Table 1:
as can be seen from the above Table 1, after two and four weeks of the treatment according to the present protocol, the residual concentration of forchlorfenuron (CPPU) is rapidly reduced, and the pulp residue is always lower than 10 mug/kg and far lower than the highest limit (1mg/kg) of the European and American residues.
As can be seen from fig. 2, the effect of the treatment scheme on the content changes of lychee pulp sucrose (view a in fig. 2), glucose (view B in fig. 2), fructose (view C in fig. 2), total sugar (view D in fig. 2) and ethanol (view E in fig. 2) and acetic acid (view F in fig. 2) which are anaerobic respiration products. Wherein the total sugar content is significantly increased over the control, while the ethanol and acetic acid content of the anaerobic respiration products is significantly reduced.
As can be seen from FIG. 3, the effect of the treatment according to this protocol on the activity of the soluble invertase gene members of the ripe fruits of Feizixiao, as well as the Soluble Neutral Invertase (SNIV) and the Soluble Acid Invertase (SAIV).
As can be seen from FIG. 4, the scheme has an influence on the expression and activity of litchi anaerobic respiration key enzyme (PDC) and Alcohol Dehydrogenase (ADH) genes.
As can be seen from the attached figures 2 to 4, the scheme can obviously inhibit the activities and gene expression of acid invertase, neutral invertase, pyruvate decarboxylase and alcohol dehydrogenase.
Example 2
This example differs from example 1 only in that: the concentration of forchlorfenuron (CPPU) was 10 mg/L.
Example 3
This example differs from example 1 only in that: the concentration of forchlorfenuron (CPPU) was 5 mg/L.
Example 4
This example differs from example 1 only in that: the concentration of forchlorfenuron (CPPU) was 2.5 mg/L.
Example 5
This example differs from example 1 only in that: and (5) utilizing clear water to treat the Feizixiao ears.
As can be seen from FIG. 1, after the treatments of examples 1 to 5, examples 1 to 3 showed significant inhibition of the sugar-reducing effect of the ripened fruits and significant inhibition of the accumulation of pulp ethanol and acetic acid within 3 weeks after ripening. Meanwhile, the gene expression and activity of sucrose catabolic enzymes (soluble neutral and acidic invertase) and anaerobic respiration key enzyme are obviously inhibited in the embodiment 1, meanwhile, the residual forchlorfenuron (CPPU) after treatment shows a descending trend, and the average pulp concentration is always lower than the European and American minimum residual limit of 1 mg/kg.
The foregoing are merely examples of the present invention and common general knowledge of known specific structures and/or features of the schemes has not been described herein in any greater detail. It should be noted that, for those skilled in the art, without departing from the structure of the present invention, several changes and modifications can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicability of the patent. The scope of the claims of the present application shall be determined by the contents of the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.
Claims (2)
1. A chemical treatment method for inhibiting the anaerobic respiration of mature litchi fruits and delaying sugar drop is characterized in that: the method comprises the following processing steps: spraying forchlorfenuron (CPPU) on the fruit cluster of the Feizixiao three weeks before the Feizixiao litchi is picked.
2. The chemical treatment method for inhibiting the anaerobic respiration of mature litchi fruits and delaying the sugar loss as claimed in claim 1, wherein the chemical treatment method comprises the following steps: the concentration of the forchlorfenuron (CPPU) is 10-20 mg/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111512234.0A CN114223424A (en) | 2021-12-07 | 2021-12-07 | Chemical treatment method for inhibiting anaerobic respiration of mature litchi fruits and delaying sugar drop |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111512234.0A CN114223424A (en) | 2021-12-07 | 2021-12-07 | Chemical treatment method for inhibiting anaerobic respiration of mature litchi fruits and delaying sugar drop |
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| Publication Number | Publication Date |
|---|---|
| CN114223424A true CN114223424A (en) | 2022-03-25 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111512234.0A Pending CN114223424A (en) | 2021-12-07 | 2021-12-07 | Chemical treatment method for inhibiting anaerobic respiration of mature litchi fruits and delaying sugar drop |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4751091A (en) * | 1986-08-15 | 1988-06-14 | University Of Hawaii | Preservation of reddish color of lychee pericarp |
| CN101692835A (en) * | 2009-09-27 | 2010-04-14 | 中国热带农业科学院南亚热带作物研究所 | Fruit retention agent as well as preparation method and use method of Feizixiao litchis |
| CN102499282A (en) * | 2011-11-23 | 2012-06-20 | 上海伊禾农产品科技发展股份有限公司 | 'Feizixiao' litchi fresh-keeping agent and preservation method |
| CN102531774A (en) * | 2011-12-22 | 2012-07-04 | 湛江师范学院 | High-efficiency anti-fruit cracking agent for longan and leechee and preparation method thereof |
| CN104429796A (en) * | 2014-12-31 | 2015-03-25 | 四川农业大学 | Grape on-tree storage method |
| WO2021168207A1 (en) * | 2020-02-20 | 2021-08-26 | Valent Biosciences Llc | Methods of improving stress tolerance, growth and yield in plants |
-
2021
- 2021-12-07 CN CN202111512234.0A patent/CN114223424A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4751091A (en) * | 1986-08-15 | 1988-06-14 | University Of Hawaii | Preservation of reddish color of lychee pericarp |
| CN101692835A (en) * | 2009-09-27 | 2010-04-14 | 中国热带农业科学院南亚热带作物研究所 | Fruit retention agent as well as preparation method and use method of Feizixiao litchis |
| CN102499282A (en) * | 2011-11-23 | 2012-06-20 | 上海伊禾农产品科技发展股份有限公司 | 'Feizixiao' litchi fresh-keeping agent and preservation method |
| CN102531774A (en) * | 2011-12-22 | 2012-07-04 | 湛江师范学院 | High-efficiency anti-fruit cracking agent for longan and leechee and preparation method thereof |
| CN104429796A (en) * | 2014-12-31 | 2015-03-25 | 四川农业大学 | Grape on-tree storage method |
| WO2021168207A1 (en) * | 2020-02-20 | 2021-08-26 | Valent Biosciences Llc | Methods of improving stress tolerance, growth and yield in plants |
Non-Patent Citations (1)
| Title |
|---|
| STERN等: "The cytokinin CPPU delays maturity in litchi cv.‘Mauritius’and extends storage-life", 《THE JOURNAL OF HORTICULTURAL SCIENCE AND BIOTECHNOLOGY》 * |
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Application publication date: 20220325 |