CN114214292A

CN114214292A - Anaerobic and aerobic probiotic symbiotic metabolic enzyme preparation and process for purifying traditional Chinese medicine protein by using same

Info

Publication number: CN114214292A
Application number: CN202111556993.7A
Authority: CN
Inventors: 王胧庆
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-12-18
Filing date: 2021-12-18
Publication date: 2022-03-22

Abstract

The invention discloses a process for purifying traditional Chinese medicine protein by using an anaerobic and aerobic probiotic symbiotic metabolic enzyme preparation, wherein the complex enzyme preparation comprises protease, lactate oxidase, pectinase, endoribonuclease, deoxyendoribonuclease, aldehyde dehydrogenase, glutamate dehydrogenase, phytase, cellulase, xylanase, citrate synthase, catalase, dismutase and auxiliary enzyme, and the process comprises the following steps of mixing the complex enzyme preparation, traditional Chinese medicine raw materials and water according to a ratio of 1-5: 500: the composite enzyme preparation is non-toxic and non-corrosive, not only harmless to organisms, but also safe and beneficial to ecological environment, and has no secondary pollution.

Description

Anaerobic and aerobic probiotic symbiotic metabolic enzyme preparation and process for purifying traditional Chinese medicine protein by using same

Technical Field

The invention relates to a process for purifying traditional Chinese medicine protein, in particular to a process for purifying traditional Chinese medicine protein by utilizing anaerobic and aerobic probiotic symbiotic metabolic enzyme preparation.

Background

The chemical components of the traditional Chinese medicine are very complex, and the traditional Chinese medicine contains various effective components, ineffective components and toxic components. Extracting the effective components of the effective monomers, and further separating and purifying the effective components to obtain the effective monomers is an important content in the research field of traditional Chinese medicines. The extraction of the traditional Chinese medicine is to extract the effective components in the traditional Chinese medicine to the maximum extent by utilizing certain technologies, so that the internal quality and the clinical treatment effect of the traditional Chinese medicine preparation are improved, and the effect of the traditional Chinese medicine is exerted to the maximum extent.

At present, the traditional purification method is adopted for purifying proteins in the traditional Chinese medicine, and generally comprises a water decoction method, an immersion method, a percolation method, a reflux method, a solvent extraction method, a steam distillation method, a sublimation method and the like. The disadvantages of such methods are: the traditional Chinese medicine effect is reduced by repeatedly heating during purification, the purification process is complex, the purification effect can be achieved even by performing three to four process flows, and toxic or ineffective components in the traditional Chinese medicine can be extracted together, so that the traditional Chinese medicine effect is reduced.

Disclosure of Invention

In order to solve the defects in the prior art, the invention aims to provide an anaerobic and aerobic probiotic symbiotic metabolic enzyme preparation and a process for purifying traditional Chinese medicine protein by using the same. The invention can shorten the time for purifying the Chinese medicine protein, reduce the process flow, increase the purification purity and improve the purity and the effect of the Chinese medicine.

The anaerobic and aerobic probiotic symbiotic metabolic enzyme preparation comprises 5-15 parts of protease, 4-9 parts of lactate oxidase, 2-8 parts of pectinase, 3-8 parts of endoribonuclease, 2-7 parts of aldehyde dehydrogenase, 1-6 parts of glutamate dehydrogenase, 2-7 parts of phytase, 2-6 parts of cellulase, 2-6 parts of xylanase, 3-8 parts of citrate synthase, 2-5 parts of catalase, 2-7 parts of dismutase and 1-8 parts of auxiliary enzyme. Wherein the auxiliary enzyme comprises 6-10 parts of mannase, 4-8 parts of glucanase, 12-18 parts of phosphokinase, 13-18 parts of acetyl coenzyme, 6-10 parts of acetyltransferase, 10-14 parts of phosphopyruvate hydratase and 10-15 parts of phosphodikinase.

The more preferable proportion of the anaerobic and aerobic probiotic symbiotic metabolic enzyme preparation is as follows: 6-12 parts of protease, 5-7 parts of lactate oxidase, 3-6 parts of pectinase, 4-5 parts of endoribonuclease, 4-6 parts of endoribonuclease, 3-6 parts of aldehyde dehydrogenase, 2-5 parts of glutamate dehydrogenase, 3-6 parts of phytase, 3-5 parts of cellulase, 3-6 parts of xylanase, 4-6 parts of citrate synthase, 3-4 parts of catalase, 3-6 parts of dismutase, 6-10 parts of mannase, 4-8 parts of glucanase, 12-18 parts of phosphokinase, 13-18 parts of acetyl coenzyme, 6-10 parts of acetyltransferase, 10-14 parts of phosphopyruvate hydratase and 10-15 parts of phosphodikinase.

The complex enzyme preparation is non-toxic, non-corrosive, harmless and non-secondary pollution, so that the complex enzyme preparation is not only harmless to organisms, but also safe and beneficial to ecological environment and free of secondary pollution.

The process for purifying the traditional Chinese medicine protein by the enzyme preparation comprises the following steps:

1) drying, weighing and slicing the traditional Chinese medicine raw materials for later use, and decocting by traditional cooking.

2) Mixing the enzyme preparation, the traditional Chinese medicine raw materials and water according to a ratio of 1-5: 500: mixing at a ratio of 1000, catalyzing for 5-10 hr under sealed condition, and hydrolyzing to activate the enzyme preparation.

3) Placing the enzyme preparation, water and traditional Chinese medicine raw materials after catalytic activation in a tank, externally heating to a critical temperature of 60-80 ℃, and reacting for 5-20 hours.

4) And conveying the converted liquid medicine to a clear liquid storage tank for precipitation.

5) Concentrating and purifying the clear liquid after precipitation to obtain the product.

The purification process mainly utilizes the enzyme preparation to rapidly break the tubular energy groups of plant raw materials such as traditional Chinese medicines and the like, and utilizes the enzyme preparation to fully catalyze and activate pectin, tannin, starch, partial acetic acid and lignin in the traditional Chinese medicine plants, thereby realizing the purification of the traditional Chinese medicine proteins. The purification process can shorten the purification time of the Chinese medicine protein, reduce the process flow, increase the purification purity and determine the effect of the Chinese medicine.

The biological enzyme preparation and the traditional Chinese medicine protein purification process thereof provided by the invention are based on the principle that the plant cell walls can be selectively destroyed by enzymolysis, so that components in the plant cells are easier to dissolve and diffuse, and the biological enzyme preparation is applied to the traditional Chinese medicine protein component extraction technology, has the advantages of high component leaching rate, reduction of degradation of thermosensitive components, low cost, capability of being completed without special equipment, and the like, has the potential of large-scale industrial production, and has obvious advantages compared with the traditional extraction technology.

The process for purifying the traditional Chinese medicine protein by using the anaerobic and aerobic probiotic symbiotic metabolic enzyme preparation has the advantages that: the enzyme preparation is prepared by fully stirring 30% of aerobic bacteria, 40% of facultative bacteria and 30% of anaerobic bacteria, and the required complex enzyme preparation is obtained, is biological enzyme and has the characteristics of no toxicity, no harm and no secondary pollution, so that the complex enzyme is used for purifying the traditional Chinese medicine protein, no potential safety hazard is caused, and the environment is not damaged; meanwhile, the compound enzyme preparation, the traditional Chinese medicine raw materials and water are mixed according to the weight ratio of 1-5: 500: 1000, the preparation is uniformly mixed, catalyzed for 5 to 10 hours under a closed condition, the enzyme preparation is fully activated by hydrolysis, the enzyme preparation, water and the traditional Chinese medicine raw materials after the activation are placed in a tank body, the raw materials are heated to the critical temperature of 60 to 80 ℃ externally and then react for 5 to 20 hours, the biological characteristics of the enzyme preparation can be effectively utilized through the processes of activation, catalysis, dissolution and the like to separate and extract the traditional Chinese medicine protein cells, the converted liquid medicine is conveyed to a clear liquid storage tank for precipitation, and the precipitated clear liquid is concentrated and purified, so that the protein in the traditional Chinese medicine can be purified to obtain the high-purity traditional Chinese medicine protein The process flow is reduced, the purification purity and the effect are increased, the traditional Chinese medicine protein purification industry is undoubtedly a revolution, and a new process method is developed for the traditional Chinese medicine protein purification.

Detailed Description

The anaerobic facultative and aerobic probiotic symbiotic metabolic enzyme preparation comprises 8 parts of protease, 6 parts of lactate oxidase, 6 parts of pectinase, 5 parts of endoribonuclease, 5 parts of deoxyendoribonuclease, 4 parts of aldehyde dehydrogenase, 3 parts of glutamate dehydrogenase, 4 parts of phytase, 4 parts of cellulase, 4 parts of xylanase, 5 parts of citrate synthase, 3 parts of catalase, 4 parts of dismutase, 4 parts of auxiliary enzyme, 7 parts of mannase, 6 parts of glucanase, 15 parts of phosphokinase, 16 parts of acetyl coenzyme, 8 parts of acetyltransferase, 12 parts of phosphopyruvate hydratase and 13 parts of phosphodikinase.

The enzyme preparation is prepared by fully stirring 30% of aerobic bacteria, 40% of facultative bacteria and 30% of anaerobic bacteria, is biological enzyme, has the characteristics of no toxicity, no harm and no secondary pollution, is harmless to organisms, is safe and beneficial to ecological environment, has no secondary pollution, does not have any potential safety hazard, and does not damage the environment. The process for purifying the traditional Chinese medicine protein by the enzyme preparation mainly comprises the steps of rapidly breaking the energy-storing groups of plant raw materials such as traditional Chinese medicines by the enzyme preparation, and fully catalyzing and activating pectin, tannin, starch, partial acetic acid and lignin in the traditional Chinese medicine by the enzyme preparation, so that the traditional Chinese medicine protein is purified. By adopting the purification process, the purification time of the traditional Chinese medicine protein can be shortened, the process flow can be reduced, the purification purity and the purification effect can be increased, the traditional Chinese medicine protein purification industry is undoubtedly revolutionary, a new process method is developed for the traditional Chinese medicine protein purification, and meanwhile, the complex enzyme preparation, the traditional Chinese medicine raw materials and the water are mixed according to the proportion of 1-5: 500: the preparation method comprises the steps of uniformly mixing the raw materials according to a proportion of 1000, catalyzing for 5-10 hours under a closed condition, fully activating an enzyme preparation by utilizing hydrolysis, placing the enzyme preparation, water and the traditional Chinese medicine raw materials which are activated by catalysis in a tank body, heating the raw materials to a critical temperature of 60-80 ℃, reacting for 5-20 hours, separating and extracting the traditional Chinese medicine protein cells by effectively utilizing the biological characteristics of the enzyme preparation through the processes of activation, catalysis, dissolution and the like, conveying the converted liquid medicine to a clear liquid storage tank for precipitation, concentrating and purifying the precipitated clear liquid, and purifying the protein in the traditional Chinese medicine to obtain the high-purity traditional Chinese medicine protein.

The invention utilizes 30% of aerobic bacteria, 40% of facultative bacteria and 30% of anaerobic bacteria to fully stir to obtain the complex enzyme preparation required by the invention, the enzyme preparation is a high-efficiency catalyst, the enzyme preparation is utilized to decompose the action object of the enzyme, active enzyme is utilized to hydrolyze certain specific substance, the invention utilizes cellulase to catalyze cellulose decomposition, protease to catalyze protein decomposition, and the complex enzyme preparation is utilized to purify Chinese medicine protein, so as to verify the components of the plant cell wall of the Chinese medicine. The plant cell wall component contains pectin, so we can use pectinase to hydrolyze it, if the cell wall is damaged, it indicates pectin in the cell wall, and this test method utilizes the specificity of enzyme, which is the principle of enzymolysis. The biological enzyme preparation is applied to the traditional Chinese medicine protein component extraction technology, has the advantages of high component leaching rate, reduction of degradation of thermosensitive components, low cost, capability of being completed without special equipment and the like, has the potential of large-scale industrial production, and has obvious advantages compared with the traditional extraction technology. Most of the bioactive components in the plant medicine exist in cells, and a small amount of the bioactive components exist in intercellular spaces. Plant cell walls mainly comprise macromolecules such as cellulose, hemicellulose, pectin and the like, and small molecular chemical components in plants penetrate into a solution and must penetrate through barriers of the plant cell walls. After the fresh medicinal materials are dried, the water in the tissues is evaporated, and the cells gradually shrink. In this case, substances such as active ingredients dissolved in the vacuole of the cell are deposited in the cell in a crystalline or amorphous state, and the cell forms a cavity, and the semipermeable property of the cytoplasmic membrane is lost, resulting in the dissolution failure of the intracellular ingredients. By utilizing the extraction method, according to the composition of the plant medicinal material cell walls, and by utilizing the characteristics of extremely high catalytic activity, high specificity and the like of the reaction of the complex enzyme preparation, the corresponding biological enzyme is selected to hydrolyze the components of the cell walls, such as cellulose, hemicellulose, pectin and the like, so that the effective components in the plant cells are easier to dissolve and diffuse.

It will be apparent to those skilled in the art that various other changes and modifications may be made in the above-described embodiments and concepts and all such changes and modifications are intended to be within the scope of the appended claims.

Claims

1. The anaerobic facultative probiotic symbiotic metabolic enzyme preparation is characterized by comprising 5-15 parts of protease, 4-9 parts of lactate oxidase, 2-8 parts of pectinase, 3-8 parts of endoribonuclease, 2-7 parts of aldehyde dehydrogenase, 1-6 parts of glutamate dehydrogenase, 2-7 parts of phytase, 2-6 parts of cellulase, 2-6 parts of xylanase, 3-8 parts of citrate synthase, 2-5 parts of catalase, 2-7 parts of dismutase and 1-8 parts of auxiliary enzyme.

2. The anaerobic and aerobic probiotic symbiotic metabolic enzyme preparation according to claim 1, wherein the enzyme preparation comprises 6-12 parts of protease, 5-7 parts of lactate oxidase, 3-6 parts of pectinase, 4-5 parts of endoribonuclease, 4-6 parts of endoribonuclease, 3-6 parts of aldehyde dehydrogenase, 2-5 parts of glutamate dehydrogenase, 3-6 parts of phytase, 3-5 parts of cellulase, 3-6 parts of xylanase, 4-6 parts of citrate synthase, 3-4 parts of catalase, 3-6 parts of dismutase and 1-8 parts of auxiliary enzyme.

3. The anaerobic and aerobic probiotic symbiotic metabolic enzyme preparation according to claim 1, characterized in that the auxiliary enzymes comprise 6-10 parts of mannanase, 4-8 parts of glucanase, 12-18 parts of phosphokinase, 13-18 parts of acetyl coenzyme, 6-10 parts of acetyltransferase, 10-14 parts of phosphopyruvate hydratase and 10-15 parts of phosphodikinase.

4. A process for purifying traditional Chinese medicine protein by using an anaerobic and aerobic probiotic symbiotic metabolic enzyme preparation is characterized by comprising the following steps: