CN114196677A - High-activity blood coagulation factor VIII or VIII a polypeptide variant Gly710Ala - Google Patents
High-activity blood coagulation factor VIII or VIII a polypeptide variant Gly710Ala Download PDFInfo
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Abstract
The invention relates to a high-activity blood coagulation factor VIII or VIII a polypeptide variant Gly710 Ala. The mutant does not destroy or inhibit the blood coagulation activity of FVIII, the specific blood coagulation activity is 1.5-2 times of that of a wild type, and the mutant is superior in stability; the mutant obviously improves the drug efficacy of FVIII and has good clinical application prospect.
Description
Technical Field
The invention belongs to the field of hemophilia, and particularly relates to a blood coagulation factor VIII or VIII a polypeptide variant Gly710Ala with high activity.
Background
Hemophilia a is an inherited bleeding disorder caused by a deficiency in blood coagulation factor viii (fviii), with an incidence of 1/5000 in the male population, and blood coagulation dysfunction in patients manifested by spontaneous muscle hematoma and joint cavity bleeding. Fviii replacement therapy is currently the only effective treatment modality. With the development of biotechnology, recombinant FVIII, which gradually replaces plasma or plasma-derived FVIII, becomes the main preparation of replacement therapy. The in vivo half-life of FVIII is only less than 12 hours, and 3 injections per week are expensive to administer in order to maintain sufficient plasma levels to prevent bleeding. The development of a FVIII preparation with higher blood coagulation activity can achieve the same treatment effect by using less dosage, reduce the generation probability of inhibitors and provide great help for clinical treatment of hemophilia A.
Disclosure of Invention
The technical problem to be solved by the invention is to provide a high-activity blood coagulation factor VIII or VIII a polypeptide variant Gly710Ala, wherein the mutant does not destroy or inhibit the blood coagulation activity of FVIII, the specific blood coagulation activity is 1.5-2 times of that of a wild type, and the stability of the mutant is more excellent; the mutant obviously improves the drug efficacy of FVIII and has good clinical application prospect.
The invention provides a nucleic acid for coding a high-activity blood coagulation factor VIII or VIII a polypeptide variant Gly710 Ala:
(1) hF VIII cDNA: the nucleotide sequence is shown in any one of SEQ ID NO 1-4;
or (2) BDD hF VIII-SQ: the nucleotide sequence is shown as any one of SEQ ID NO 5-8;
or (3) BDD hFVIII-N8: the nucleotide sequence is shown in any one of SEQ ID NO 9-12;
or (4) BDD hFVIII-SC: the nucleotide sequence is shown in any one of SEQ ID NO 13-16;
or (5) BDD hF VIII- Δ F: the nucleotide sequence is shown in any one of SEQ ID NO 17-20.
The invention also provides a mutant protein of a high-activity blood coagulation factor VIII or VIII a polypeptide variant Gly710Ala, the amino acid sequence of the mutant is shown in any one of SEQ ID NO 21-29, and the 710 th amino acid of the mutant is Ala but not Gly of wild type VIII or VIII a. The amino acid sequence sequentially comprises the amino acid sequences of the corresponding mutant proteins of hFVIII cDNA, BDHFVIII-SQ, BDHFVIII-N8, BDHFVIII-SC and BDHFVIII-delta F and the amino acid sequences of the mutant proteins of BDHFVIII-V3, BDD human-cl rhFVIII, pFVIII and BDD pFVIII-OL.
The invention also provides a nucleic acid encoding the mutant protein, or a nucleic acid which has the same length as the encoding nucleic acid and is completely complementary with the encoding nucleic acid.
The invention also provides a carrier for expressing the mutant protein.
The invention also provides a preparation method of the mutant protein of the polypeptide variant Gly710Ala of the high-activity blood coagulation factor VIII or VIII a, which comprises the following steps:
BDD-hFVIII or full-length FVIII is taken as a template, a PCR fixed-point mutagenesis method is used for introducing mutation sites into wild FVIII, Gly710 amino acid residues are replaced by Ala, and BDD-hFVIII or full-length FVIII eukaryotic expression plasmids which are correctly inserted with Gly710Ala mutation are screened after conversion.
The invention also provides application of the mutant protein coding nucleic acid of the high-activity blood coagulation factor VIII or VIII a polypeptide variant Gly710Ala, and the application of the nucleic acid in preparing gene therapy medicaments comprises the steps of connecting the nucleic acid with a promoter and a termination sequence, constructing an expression plasmid, a gene therapy virus or a non-viral vector and expressing the mutant protein.
The invention also provides application of the mutant protein of the high-activity blood coagulation factor VIII or VIII a polypeptide variant Gly710Ala in preparation of a recombinant protein treatment medicament for hemophilia.
The invention also provides application of the mutant protein of the high-activity blood coagulation factor VIII or VIII a polypeptide variant Gly710Ala, which is applied to preparing the fusion protein of the blood coagulation factor VIII or VIII a polypeptide variant Gly710Ala mutant and used for preparing a recombinant protein treatment medicament for hemophilia.
The fusion protein is obtained by fusing one of human albumin, immunoglobulin Fc, transferrin or alpha 1 antitrypsin with the mutant protein of the polypeptide variant Gly710Ala of the blood coagulation factor VIII or VIII a.
The nucleic acid or amino acid sequence pharmaceutical composition or gene therapy vector of the present invention is useful for preventing and/or treating diseases, wherein the diseases mainly include hemorrhagic diseases or hemorrhage due to various causes; among the most likely bleeding disorders are hemophilia a and B, i.e. bleeding disorders due to hereditary factor viii deficiency, and include hemophilia a and B in which inhibitory antibodies are produced, or acquired factor viii deficiency due to the production of inhibitors; and other hemorrhagic diseases using bypass preparations, such as neonatal coagulation disorders; severe liver disease; high risk surgery; traumatic blood loss; bone marrow transplantation; thrombocytopenia and platelet dysfunction; emergency reversal of oral anticoagulation; a deficiency in congenital factor viii; von willebrand disease, and acquired von willebrand disease caused by inhibitors of von willebrand factor, blood loss associated with extensive injury, cerebral hemorrhage, platelet dysfunction.
Advantageous effects
The mutant does not destroy or inhibit the blood coagulation activity of FVIII, the specific blood coagulation activity is 1.5-2 times of that of a wild type, and the mutant is superior in stability; the mutant obviously improves the drug efficacy of FVIII and has good clinical application prospect.
Drawings
FIG. 1 shows the time course of FVIII activity of BDD-hFVIII Gly710Ala mutants in the absence of FIXa.
FIG. 2 shows the time course of FVIII activity of BDD-hFVIII Gly710Ala mutant in the presence of FIXa.
FIG. 3 shows the BDD-hFVIII Gly710Ala mutant thermal stability assay.
Detailed Description
The invention will be further illustrated with reference to the following specific examples. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Further, it should be understood that various changes or modifications of the present invention may be made by those skilled in the art after reading the teaching of the present invention, and such equivalents may fall within the scope of the present invention as defined in the appended claims.
Example 1
A mutant protein of a polypeptide variant Gly710Ala of high-activity blood coagulation factor VIII or VIII a, wherein the amino acid sequence of the mutant is shown in any one of SEQ ID NO 21-29, and the 710 th amino acid of the mutant is Ala but not Gly of wild type VIII or VIII a. In this example, muteins of hFVIII cDNA were used as examples.
Expression and purification of FVIII Gly710Ala mutants
(1) Expressing: site-directed mutagenesis kit (using QuikChange: (9))
Ⅱ XL Site-Directed Mutagenesis Kit,Agilent, USA), using human B-region deletion type blood coagulation VIII factor (BDD-hFVIII) as a template, introducing mutation sites into wild type FVIII by a PCR (polymerase chain reaction) fixed-point mutagenesis method, replacing 710Gly with 710Ala, digesting amplification products by Dpn I, then converting the amplification products (adding 5-10 microliter of mutation products digested by Dpn I into 100 microliter of competent bacteria), coating the converted bacteria on a plate containing 1/2000 ampicillin, and culturing overnight to obtain clones; selecting a single colony, inoculating the single colony to 5ml LB culture solution containing 1/2000 ampicillin, carrying out overnight strain amplification on a shaking table at the temperature of 37 ℃ at the speed of 250 rpm, extracting plasmid DNA for sequencing, and screening BDD-hFVIII eukaryotic expression plasmids which are correctly inserted with Gly710Ala mutation.
(2) And (3) purification: and transfecting CHO cells by using wild BDD-hFVIII and the constructed BDD-hFVIII Gly710Ala expression plasmids respectively, and establishing a blank vector control. After the stable high-expression clone cell strain is screened out, the clone cell strain is transferred into a selective serum-free culture solution for large-scale culture, and the FVIII secreted into the culture solution is purified by an immunoadsorption method (immunoadsorption gel VIII-Select, GE Healthcare, USA). The product was concentrated using Centricon Plus Ultrafiltration centrifuge tubes (MILLIPORE, USA).
Detection of coagulation Activity of FVIII Gly710Ala mutant
(1) Coenzyme Activity assay of FVIII Gly710Ala mutant (FXa production assay): the coactivator activity of FVIII is manifested as the ability of FIXa to assist in the cleavage of FX to FXa. The wild-type BDD-hfviii was used as a control, and the coenzyme activity of the fviii Gly710Ala mutant was assessed using FXa generation assay: thrombin acts on FVIII for 1min (FVIII: thrombin: 20:1) to activate FVIII to FVIII a, hirudin inactivates thrombin; 0.5nM of FVIIIa is taken, 20 mu M, FIXa40nM of PSPC phospholipid particles are added, mixed and incubated for 1min at 37 ℃, FX with different concentrations of 0nM, 20nM, 40nM, 60nM, 120nM, 240nM and the like is respectively added, reaction is carried out for 10min at 37 ℃, and EDTA stops the reaction; adding FXa specific chromogenic substrate S-2765, carrying out color comparison at the wavelength of 405nm, and measuring the generated FXa. Michaelis-Menten enzyme kinetic analysis was performed using graphpad prism software, and the enzyme kinetic constants Km, Kcat, and Vmax were calculated.
(2) Effect of fviii Gly710Ala mutant on specific activity of fviii: specific Generation of FVIIIThe coagulation activity of FVIII per mg of protein is shown in the table. The activity of the purified BDD-hFVIII mutant and wild type was determined using a first-stage and a second-stage method (Chromogenissay) according to
SP4F VIII kit (Chromogenix, Italy) protocol. Protein quantification with enzyme-linked immunosorbent assay: the concentration of the sample was determined by sandwich ELISA using anti-C2 antibody GMA-8014 as the capture antibody, biotin-labeled anti-A2 antibody GMA-8016 as the detection antibody, and purified recombinant human FVIII as the standard. The specific activity of the FVIII mutant and the relative activity of the FVIII mutant relative to the wild type were calculated.
Effect of FVIII Gly710Ala mutants on FVIII stability
(1) Viii thermal stability test: the stability of wild type FVIII was assessed by observing the extent of loss of activity of the FVIII Gly710Ala mutant under high temperature conditions. Respectively taking 4nM of wild type FVIII and FVIII mutants, incubating at 55 ℃, respectively sampling at 0min, 10min, 20min, 30min and 40min after incubation, detecting residual FVIII activity by applying a chromogenic substrate method, and analyzing by graphpadprist software to obtain a one phase decay model fitting attenuation curve to obtain a half-life period.
(2) Fviia activity decay assay:
the time course of FVIII activity in the absence of FIXa: respectively taking 4nM of F VIII Gly710Ala and wild BDD-hF VIII, inactivating thrombin by hirudin after 20nM activation of thrombin, adding FIXa40nM and PSPC 10 mu M, mixing, incubating at 23 ℃, respectively sampling at 0min, 5min, 10min, 15min and 20min after incubation, adding FX, detecting the concentration of FXa in a sample according to an FXa generation test program, and indirectly determining the concentration of residual F VIII a.
Interval of FVIII activity in the presence of FIXa: the above procedure was repeated, and factor IXa40nM was added to the reaction before thrombin was added. Inactivating thrombin by hirudin activated by 20nM thrombin, adding 10 μ M PSPC, mixing, incubating at 23 deg.C, sampling respectively at 0min, 10min, 20min, 30min and 40min after incubation, adding FX, detecting FXa concentration of sample by comparison with FXa generation test program, and indirectly determining the concentration of residual FVIIia.
And thirdly, fitting an attenuation curve by using a one phase decay model of the graphpad prism to obtain a half-life period, and comparing the stability of the FVIIia.
4. Results of the experiment
(1) BDD-hF VIII Gly710Ala mutant relative ratio activity detection:
transient transfection of plasmid into CHO cell, culturing for 48 hr, collecting culture liquid, taking supernatant for detection, detecting the activity of the mutant by APTT one-stage method, detecting the protein amount of the mutant by ELISA method, comparing with wild BDD-hF VIII, and obtaining the relative specific activity of the mutant primarily. The results show that: in 8 experiments, the relative specific activity of BDD-hFVIII Gly710Ala mutant was 1.597 + -0.402.
Screening cell strains stably expressed by the BDD-hFVIII Gly710Ala mutant to carry out high-capacity cell culture, collecting culture solutions in 48h and 72h respectively, and extracting, purifying and concentrating at low temperature to obtain the target protein. And respectively detecting the activity of the mutant by a first-stage method and a second-stage method, detecting the protein amount of the mutant by an ELISA method, and comparing the protein amount with the wild BDD-hFVIII to calculate the relative specific activity of the mutant. The results show that: in 6 experiments, the relative ratio activity of the BDD-hFVIII Gly710Ala mutant in the first phase method is 2.214 + -0.767, and the relative ratio activity in the second phase method is 1.521 + -0.068.
(2) And (3) detecting the coenzyme activity and stability of the BDD-hFVIII Gly710Ala mutant:
the high-capacity cell culture product is adopted for detection, and the coenzyme activity and stability of the mutant are detected through FXa generation test, FVIII activity time course test and the like, and the result shows that the mutant not only has the coenzyme performance and stability equivalent to those of a wild type under the same activity concentration, but also has better stability in the presence of FIXa.
The coenzyme action of the F VIII Gly710Ala mutant is detected by an FXa generation test. The results show that: after the F VIII Gly710 site is mutated into Ala, good coenzyme performance is still shown.
② the coenzyme state and stability of the FVIII Gly710Ala mutant are detected by the activity time course of the FVIII without IXa. As shown in the results of fig. 1: in the FIXa-free reaction, the activity of FVIII Gly710Ala mutant and wild BDD-hFVIII at 23 ℃ decayed approximately 50% of time, suggesting that the two have similar coenzyme states, A2 subunit retention rate and stability.
And thirdly, observing the influence of the FVIII mutant on the stability of the FVIII by detecting the time course of the FVIII activity in the presence of FIXa. As shown in the results of fig. 2: in the FIXa-containing reaction, the 50% decay time of the FVIII Gly710Ala mutant activity at 23 ℃ was about 1.5 times that of the wild type. The mutant is suggested to have higher stability than the wild type under the action of the binding enzyme FIXa.
And fourthly, testing the heat stability of the BDD-hF VIII Gly710Ala mutant: as shown in the results of FIG. 3, the activity of the FVIII Gly710Ala mutant decayed by 50% compared to the wild type BDD at 55 ℃ indicating that the two have similar thermostability at high temperature.
Sequence listing
<110> Guangxi medical university first subsidiary hospital
<120> a highly active factor VIII or VIIa polypeptide variant Gly710Ala
<130> 1
<160> 29
<170> SIPOSequenceListing 1.0
<210> 1
<211> 7056
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
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ggtgagctgc ctgtggacgc aagatttcct cctagagtgc caaaatcttt tccattcaac 180
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gctaagccaa ggccaccctg gatgggtctg ctaggtccta ccatccaggc tgaggtttat 300
gatacagtgg tcattacact taagaacatg gcttcccatc ctgtcagtct tcatgctgtt 360
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gctgcatctg ctcgggcctg gcctaaaatg cacacagtca atggttatgt aaacaggtct 780
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cagcttgagg atccagagtt ccaagcctcc aacatcatgc acagcatcaa tggctatgtt 1920
tttgatagtt tgcagttgtc agtttgtttg catgaggtgg catactggta cattctaagc 1980
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atggtctatg aagacacact caccctattc ccattctcag gagaaactgt cttcatgtcg 2100
atggaaaacc caggtctatg gattctggct tgccacaact cagactttcg gaacagaggc 2160
atgaccgcct tactgaaggt ttctagttgt gacaagaaca ctggtgatta ttacgaggac 2220
agttatgaag atatttcagc atacttgctg agtaaaaaca atgccattga accaagaagc 2280
ttctcccaga attcaagaca ccctagcact aggcaaaagc aatttaatgc caccacaatt 2340
ccagaaaatg acatagagaa gactgaccct tggtttgcac acagaacacc tatgcctaaa 2400
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aaactgggga caactgcagc aacagagttg aagaaacttg atttcaaagt ttctagtaca 2700
tcaaataatc tgatttcaac aattccatca gacaatttgg cagcaggtac tgataataca 2760
agttccttag gacccccaag tatgccagtt cattatgata gtcaattaga taccactcta 2820
tttggcaaaa agtcatctcc ccttactgag tctggtggac ctctgagctt gagtgaagaa 2880
aataatgatt caaagttgtt agaatcaggt ttaatgaata gccaagaaag ttcatgggga 2940
aaaaatgtat cgtcaacaga gagtggtagg ttatttaaag ggaaaagagc tcatggacct 3000
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aaaacttcca ataattcagc aactaataga aagactcaca ttgatggccc atcattatta 3120
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gtgacacctt tgattcatga cagaatgctt atggacaaaa atgctacagc tttgaggcta 3240
aatcatatgt caaataaaac tacttcatca aaaaacatgg aaatggtcca acagaaaaaa 3300
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caaggcccca gtccaaagca attagtatcc ttaggaccag aaaaatctgt ggaaggtcag 3480
aatttcttgt ctgagaaaaa caaagtggta gtaggaaagg gtgaatttac aaaggacgta 3540
ggactcaaag agatggtttt tccaagcagc agaaacctat ttcttactaa cttggataat 3600
ttacatgaaa ataatacaca caatcaagaa aaaaaaattc aggaagaaat agaaaagaag 3660
gaaacattaa tccaagagaa tgtagttttg cctcagatac atacagtgac tggcactaag 3720
aatttcatga agaacctttt cttactgagc actaggcaaa atgtagaagg ttcatatgac 3780
ggggcatatg ctccagtact tcaagatttt aggtcattaa atgattcaac aaatagaaca 3840
aagaaacaca cagctcattt ctcaaaaaaa ggggaggaag aaaacttgga aggcttggga 3900
aatcaaacca agcaaattgt agagaaatat gcatgcacca caaggatatc tcctaataca 3960
agccagcaga attttgtcac gcaacgtagt aagagagctt tgaaacaatt cagactccca 4020
ctagaagaaa cagaacttga aaaaaggata attgtggatg acacctcaac ccagtggtcc 4080
aaaaacatga aacatttgac cccgagcacc ctcacacaga tagactacaa tgagaaggag 4140
aaaggggcca ttactcagtc tcccttatca gattgcctta cgaggagtca tagcatccct 4200
caagcaaata gatctccatt acccattgca aaggtatcat catttccatc tattagacct 4260
atatatctga ccagggtcct attccaagac aactcttctc atcttccagc agcatcttat 4320
agaaagaaag attctggggt ccaagaaagc agtcatttct tacaaggagc caaaaaaaat 4380
aacctttctt tagccattct aaccttggag atgactggtg atcaaagaga ggttggctcc 4440
ctggggacaa gtgccacaaa ttcagtcaca tacaagaaag ttgagaacac tgttctcccg 4500
aaaccagact tgcccaaaac atctggcaaa gttgaattgc ttccaaaagt tcacatttat 4560
cagaaggacc tattccctac ggaaactagc aatgggtctc ctggccatct ggatctcgtg 4620
gaagggagcc ttcttcaggg aacagaggga gcgattaagt ggaatgaagc aaacagacct 4680
ggaaaagttc cctttctgag agtagcaaca gaaagctctg caaagactcc ctccaagcta 4740
ttggatcctc ttgcttggga taaccactat ggtactcaga taccaaaaga agagtggaaa 4800
tcccaagaga agtcaccaga aaaaacagct tttaagaaaa aggataccat tttgtccctg 4860
aacgcttgtg aaagcaatca tgcaatagca gcaataaatg agggacaaaa taagcccgaa 4920
atagaagtca cctgggcaaa gcaaggtagg actgaaaggc tgtgctctca aaacccacca 4980
gtcttgaaac gccatcaacg ggaaataact cgtactactc ttcagtcaga tcaagaggaa 5040
attgactatg atgataccat atcagttgaa atgaagaagg aagattttga catttatgat 5100
gaggatgaaa atcagagccc ccgcagcttt caaaagaaaa cacgacacta ttttattgct 5160
gcagtggaga ggctctggga ttatgggatg agtagctccc cacatgttct aagaaacagg 5220
gctcagagtg gcagtgtccc tcagttcaag aaagttgttt tccaggaatt tactgatggc 5280
tcctttactc agcccttata ccgtggagaa ctaaatgaac atttgggact cctggggcca 5340
tatataagag cagaagttga agataatatc atggtaactt tcagaaatca ggcctctcgt 5400
ccctattcct tctattctag ccttatttct tatgaggaag atcagaggca aggagcagaa 5460
cctagaaaaa actttgtcaa gcctaatgaa accaaaactt acttttggaa agtgcaacat 5520
catatggcac ccactaaaga tgagtttgac tgcaaagcct gggcttattt ctctgatgtt 5580
gacctggaaa aagatgtgca ctcaggcctg attggacccc ttctggtctg ccacactaac 5640
acactgaacc ctgctcatgg gagacaagtg acagtacagg aatttgctct gtttttcacc 5700
atctttgatg agaccaaaag ctggtacttc actgaaaata tggaaagaaa ctgcagggct 5760
ccctgcaata tccagatgga agatcccact tttaaagaga attatcgctt ccatgcaatc 5820
aatggctaca taatggatac actacctggc ttagtaatgg ctcaggatca aaggattcga 5880
tggtatctgc tcagcatggg cagcaatgaa aacatccatt ctattcattt cagtggacat 5940
gtgttcactg tacgaaaaaa agaggagtat aaaatggcac tgtacaatct ctatccaggt 6000
gtttttgaga cagtggaaat gttaccatcc aaagctggaa tttggcgggt ggaatgcctt 6060
attggcgagc atctacatgc tgggatgagc acactttttc tggtgtacag caataagtgt 6120
cagactcccc tgggaatggc ttctggacac attagagatt ttcagattac agcttcagga 6180
caatatggac agtgggcccc aaagctggcc agacttcatt attccggatc aatcaatgcc 6240
tggagcacca aggagccctt ttcttggatc aaggtggatc tgttggcacc aatgattatt 6300
cacggcatca agacccaggg tgcccgtcag aagttctcca gcctctacat ctctcagttt 6360
atcatcatgt atagtcttga tgggaagaag tggcagactt atcgaggaaa ttccactgga 6420
accttaatgg tcttctttgg caatgtggat tcatctggga taaaacacaa tatttttaac 6480
cctccaatta ttgctcgata catccgtttg cacccaactc attatagcat tcgcagcact 6540
cttcgcatgg agttgatggg ctgtgattta aatagttgca gcatgccatt gggaatggag 6600
agtaaagcaa tatcagatgc acagattact gcttcatcct actttaccaa tatgtttgcc 6660
acctggtctc cttcaaaagc tcgacttcac ctccaaggga ggagtaatgc ctggagacct 6720
caggtgaata atccaaaaga gtggctgcaa gtggacttcc agaagacaat gaaagtcaca 6780
ggagtaacta ctcagggagt aaaatctctg cttaccagca tgtatgtgaa ggagttcctc 6840
atctccagca gtcaagatgg ccatcagtgg actctctttt ttcagaatgg caaagtaaag 6900
gtttttcagg gaaatcaaga ctccttcaca cctgtggtga actctctaga cccaccgtta 6960
ctgactcgct accttcgaat tcacccccag agttgggtgc accagattgc cctgaggatg 7020
gaggttctgg gctgcgaggc acaggacctc tactag 7056
<210> 2
<211> 7056
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 2
atgcaaatag agctctccac ctgcttcttt ctgtgccttt tgcgattctg ctttagtgcc 60
accagaagat actacctggg tgcagtggaa ctgtcatggg actatatgca aagtgatctc 120
ggtgagctgc ctgtggacgc aagatttcct cctagagtgc caaaatcttt tccattcaac 180
acctcagtcg tgtacaaaaa gactctgttt gtagaattca cggatcacct tttcaacatc 240
gctaagccaa ggccaccctg gatgggtctg ctaggtccta ccatccaggc tgaggtttat 300
gatacagtgg tcattacact taagaacatg gcttcccatc ctgtcagtct tcatgctgtt 360
ggtgtatcct actggaaagc ttctgaggga gctgaatatg atgatcagac cagtcaaagg 420
gagaaagaag atgataaagt cttccctggt ggaagccata catatgtctg gcaggtcctg 480
aaagagaatg gtccaatggc ctctgaccca ctgtgcctta cctactcata tctttctcat 540
gtggacctgg taaaagactt gaattcaggc ctcattggag ccctactagt atgtagagaa 600
gggagtctgg ccaaggaaaa gacacagacc ttgcacaaat ttatactact ttttgctgta 660
tttgatgaag ggaaaagttg gcactcagaa acaaagaact ccttgatgca ggatagggat 720
gctgcatctg ctcgggcctg gcctaaaatg cacacagtca atggttatgt aaacaggtct 780
ctgccaggtc tgattggatg ccacaggaaa tcagtctatt ggcatgtgat tggaatgggc 840
accactcctg aagtgcactc aatattcctc gaaggtcaca catttcttgt gaggaaccat 900
cgccaggcgt ccttggaaat ctcgccaata actttcctta ctgctcaaac actcttgatg 960
gaccttggac agtttctact gttttgtcat atctcttccc accaacatga tggcatggaa 1020
gcttatgtca aagtagacag ctgtccagag gaaccccaac tacgaatgaa aaataatgaa 1080
gaagcggaag actatgatga tgatcttact gattctgaaa tggatgtggt caggtttgat 1140
gatgacaact ctccttcctt tatccaaatt cgctcagttg ccaagaagca tcctaaaact 1200
tgggtacatt acattgctgc tgaagaggag gactgggact atgctccctt agtcctcgcc 1260
cccgatgaca gaagttataa aagtcaatat ttgaacaatg gccctcagcg gattggtagg 1320
aagtacaaaa aagtccgatt tatggcatac acagatgaaa cctttaagac tcgtgaagct 1380
attcagcatg aatcaggaat cttgggacct ttactttatg gggaagttgg agacacactg 1440
ttgattatat ttaagaatca agcaagcaga ccatataaca tctaccctca cggaatcact 1500
gatgtccgtc ctttgtattc aaggagatta ccaaaaggtg taaaacattt gaaggatttt 1560
ccaattctgc caggagaaat attcaaatat aaatggacag tgactgtaga agatgggcca 1620
actaaatcag atcctcggtg cctgacccgc tattactcta gtttcgttaa tatggagaga 1680
gatctagctt caggactcat tggccctctc ctcatctgct acaaagaatc tgtagatcaa 1740
agaggaaacc agataatgtc agacaagagg aatgtcatcc tgttttctgt atttgatgag 1800
aaccgaagct ggtacctcac agagaatata caacgctttc tccccaatcc agctggagtg 1860
cagcttgagg atccagagtt ccaagcctcc aacatcatgc acagcatcaa tggctatgtt 1920
tttgatagtt tgcagttgtc agtttgtttg catgaggtgg catactggta cattctaagc 1980
attggagcac agactgactt cctttctgtc ttcttctctg gatatacctt caaacacaaa 2040
atggtctatg aagacacact caccctattc ccattctcag gagaaactgt cttcatgtcg 2100
atggaaaacc caggtctatg gattctggcc tgccacaact cagactttcg gaacagaggc 2160
atgaccgcct tactgaaggt ttctagttgt gacaagaaca ctggtgatta ttacgaggac 2220
agttatgaag atatttcagc atacttgctg agtaaaaaca atgccattga accaagaagc 2280
ttctcccaga attcaagaca ccctagcact aggcaaaagc aatttaatgc caccacaatt 2340
ccagaaaatg acatagagaa gactgaccct tggtttgcac acagaacacc tatgcctaaa 2400
atacaaaatg tctcctctag tgatttgttg atgctcttgc gacagagtcc tactccacat 2460
gggctatcct tatctgatct ccaagaagcc aaatatgaga ctttttctga tgatccatca 2520
cctggagcaa tagacagtaa taacagcctg tctgaaatga cacacttcag gccacagctc 2580
catcacagtg gggacatggt atttacccct gagtcaggcc tccaattaag attaaatgag 2640
aaactgggga caactgcagc aacagagttg aagaaacttg atttcaaagt ttctagtaca 2700
tcaaataatc tgatttcaac aattccatca gacaatttgg cagcaggtac tgataataca 2760
agttccttag gacccccaag tatgccagtt cattatgata gtcaattaga taccactcta 2820
tttggcaaaa agtcatctcc ccttactgag tctggtggac ctctgagctt gagtgaagaa 2880
aataatgatt caaagttgtt agaatcaggt ttaatgaata gccaagaaag ttcatgggga 2940
aaaaatgtat cgtcaacaga gagtggtagg ttatttaaag ggaaaagagc tcatggacct 3000
gctttgttga ctaaagataa tgccttattc aaagttagca tctctttgtt aaagacaaac 3060
aaaacttcca ataattcagc aactaataga aagactcaca ttgatggccc atcattatta 3120
attgagaata gtccatcagt ctggcaaaat atattagaaa gtgacactga gtttaaaaaa 3180
gtgacacctt tgattcatga cagaatgctt atggacaaaa atgctacagc tttgaggcta 3240
aatcatatgt caaataaaac tacttcatca aaaaacatgg aaatggtcca acagaaaaaa 3300
gagggcccca ttccaccaga tgcacaaaat ccagatatgt cgttctttaa gatgctattc 3360
ttgccagaat cagcaaggtg gatacaaagg actcatggaa agaactctct gaactctggg 3420
caaggcccca gtccaaagca attagtatcc ttaggaccag aaaaatctgt ggaaggtcag 3480
aatttcttgt ctgagaaaaa caaagtggta gtaggaaagg gtgaatttac aaaggacgta 3540
ggactcaaag agatggtttt tccaagcagc agaaacctat ttcttactaa cttggataat 3600
ttacatgaaa ataatacaca caatcaagaa aaaaaaattc aggaagaaat agaaaagaag 3660
gaaacattaa tccaagagaa tgtagttttg cctcagatac atacagtgac tggcactaag 3720
aatttcatga agaacctttt cttactgagc actaggcaaa atgtagaagg ttcatatgac 3780
ggggcatatg ctccagtact tcaagatttt aggtcattaa atgattcaac aaatagaaca 3840
aagaaacaca cagctcattt ctcaaaaaaa ggggaggaag aaaacttgga aggcttggga 3900
aatcaaacca agcaaattgt agagaaatat gcatgcacca caaggatatc tcctaataca 3960
agccagcaga attttgtcac gcaacgtagt aagagagctt tgaaacaatt cagactccca 4020
ctagaagaaa cagaacttga aaaaaggata attgtggatg acacctcaac ccagtggtcc 4080
aaaaacatga aacatttgac cccgagcacc ctcacacaga tagactacaa tgagaaggag 4140
aaaggggcca ttactcagtc tcccttatca gattgcctta cgaggagtca tagcatccct 4200
caagcaaata gatctccatt acccattgca aaggtatcat catttccatc tattagacct 4260
atatatctga ccagggtcct attccaagac aactcttctc atcttccagc agcatcttat 4320
agaaagaaag attctggggt ccaagaaagc agtcatttct tacaaggagc caaaaaaaat 4380
aacctttctt tagccattct aaccttggag atgactggtg atcaaagaga ggttggctcc 4440
ctggggacaa gtgccacaaa ttcagtcaca tacaagaaag ttgagaacac tgttctcccg 4500
aaaccagact tgcccaaaac atctggcaaa gttgaattgc ttccaaaagt tcacatttat 4560
cagaaggacc tattccctac ggaaactagc aatgggtctc ctggccatct ggatctcgtg 4620
gaagggagcc ttcttcaggg aacagaggga gcgattaagt ggaatgaagc aaacagacct 4680
ggaaaagttc cctttctgag agtagcaaca gaaagctctg caaagactcc ctccaagcta 4740
ttggatcctc ttgcttggga taaccactat ggtactcaga taccaaaaga agagtggaaa 4800
tcccaagaga agtcaccaga aaaaacagct tttaagaaaa aggataccat tttgtccctg 4860
aacgcttgtg aaagcaatca tgcaatagca gcaataaatg agggacaaaa taagcccgaa 4920
atagaagtca cctgggcaaa gcaaggtagg actgaaaggc tgtgctctca aaacccacca 4980
gtcttgaaac gccatcaacg ggaaataact cgtactactc ttcagtcaga tcaagaggaa 5040
attgactatg atgataccat atcagttgaa atgaagaagg aagattttga catttatgat 5100
gaggatgaaa atcagagccc ccgcagcttt caaaagaaaa cacgacacta ttttattgct 5160
gcagtggaga ggctctggga ttatgggatg agtagctccc cacatgttct aagaaacagg 5220
gctcagagtg gcagtgtccc tcagttcaag aaagttgttt tccaggaatt tactgatggc 5280
tcctttactc agcccttata ccgtggagaa ctaaatgaac atttgggact cctggggcca 5340
tatataagag cagaagttga agataatatc atggtaactt tcagaaatca ggcctctcgt 5400
ccctattcct tctattctag ccttatttct tatgaggaag atcagaggca aggagcagaa 5460
cctagaaaaa actttgtcaa gcctaatgaa accaaaactt acttttggaa agtgcaacat 5520
catatggcac ccactaaaga tgagtttgac tgcaaagcct gggcttattt ctctgatgtt 5580
gacctggaaa aagatgtgca ctcaggcctg attggacccc ttctggtctg ccacactaac 5640
acactgaacc ctgctcatgg gagacaagtg acagtacagg aatttgctct gtttttcacc 5700
atctttgatg agaccaaaag ctggtacttc actgaaaata tggaaagaaa ctgcagggct 5760
ccctgcaata tccagatgga agatcccact tttaaagaga attatcgctt ccatgcaatc 5820
aatggctaca taatggatac actacctggc ttagtaatgg ctcaggatca aaggattcga 5880
tggtatctgc tcagcatggg cagcaatgaa aacatccatt ctattcattt cagtggacat 5940
gtgttcactg tacgaaaaaa agaggagtat aaaatggcac tgtacaatct ctatccaggt 6000
gtttttgaga cagtggaaat gttaccatcc aaagctggaa tttggcgggt ggaatgcctt 6060
attggcgagc atctacatgc tgggatgagc acactttttc tggtgtacag caataagtgt 6120
cagactcccc tgggaatggc ttctggacac attagagatt ttcagattac agcttcagga 6180
caatatggac agtgggcccc aaagctggcc agacttcatt attccggatc aatcaatgcc 6240
tggagcacca aggagccctt ttcttggatc aaggtggatc tgttggcacc aatgattatt 6300
cacggcatca agacccaggg tgcccgtcag aagttctcca gcctctacat ctctcagttt 6360
atcatcatgt atagtcttga tgggaagaag tggcagactt atcgaggaaa ttccactgga 6420
accttaatgg tcttctttgg caatgtggat tcatctggga taaaacacaa tatttttaac 6480
cctccaatta ttgctcgata catccgtttg cacccaactc attatagcat tcgcagcact 6540
cttcgcatgg agttgatggg ctgtgattta aatagttgca gcatgccatt gggaatggag 6600
agtaaagcaa tatcagatgc acagattact gcttcatcct actttaccaa tatgtttgcc 6660
acctggtctc cttcaaaagc tcgacttcac ctccaaggga ggagtaatgc ctggagacct 6720
caggtgaata atccaaaaga gtggctgcaa gtggacttcc agaagacaat gaaagtcaca 6780
ggagtaacta ctcagggagt aaaatctctg cttaccagca tgtatgtgaa ggagttcctc 6840
atctccagca gtcaagatgg ccatcagtgg actctctttt ttcagaatgg caaagtaaag 6900
gtttttcagg gaaatcaaga ctccttcaca cctgtggtga actctctaga cccaccgtta 6960
ctgactcgct accttcgaat tcacccccag agttgggtgc accagattgc cctgaggatg 7020
gaggttctgg gctgcgaggc acaggacctc tactag 7056
<210> 3
<211> 7056
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 3
atgcaaatag agctctccac ctgcttcttt ctgtgccttt tgcgattctg ctttagtgcc 60
accagaagat actacctggg tgcagtggaa ctgtcatggg actatatgca aagtgatctc 120
ggtgagctgc ctgtggacgc aagatttcct cctagagtgc caaaatcttt tccattcaac 180
acctcagtcg tgtacaaaaa gactctgttt gtagaattca cggatcacct tttcaacatc 240
gctaagccaa ggccaccctg gatgggtctg ctaggtccta ccatccaggc tgaggtttat 300
gatacagtgg tcattacact taagaacatg gcttcccatc ctgtcagtct tcatgctgtt 360
ggtgtatcct actggaaagc ttctgaggga gctgaatatg atgatcagac cagtcaaagg 420
gagaaagaag atgataaagt cttccctggt ggaagccata catatgtctg gcaggtcctg 480
aaagagaatg gtccaatggc ctctgaccca ctgtgcctta cctactcata tctttctcat 540
gtggacctgg taaaagactt gaattcaggc ctcattggag ccctactagt atgtagagaa 600
gggagtctgg ccaaggaaaa gacacagacc ttgcacaaat ttatactact ttttgctgta 660
tttgatgaag ggaaaagttg gcactcagaa acaaagaact ccttgatgca ggatagggat 720
gctgcatctg ctcgggcctg gcctaaaatg cacacagtca atggttatgt aaacaggtct 780
ctgccaggtc tgattggatg ccacaggaaa tcagtctatt ggcatgtgat tggaatgggc 840
accactcctg aagtgcactc aatattcctc gaaggtcaca catttcttgt gaggaaccat 900
cgccaggcgt ccttggaaat ctcgccaata actttcctta ctgctcaaac actcttgatg 960
gaccttggac agtttctact gttttgtcat atctcttccc accaacatga tggcatggaa 1020
gcttatgtca aagtagacag ctgtccagag gaaccccaac tacgaatgaa aaataatgaa 1080
gaagcggaag actatgatga tgatcttact gattctgaaa tggatgtggt caggtttgat 1140
gatgacaact ctccttcctt tatccaaatt cgctcagttg ccaagaagca tcctaaaact 1200
tgggtacatt acattgctgc tgaagaggag gactgggact atgctccctt agtcctcgcc 1260
cccgatgaca gaagttataa aagtcaatat ttgaacaatg gccctcagcg gattggtagg 1320
aagtacaaaa aagtccgatt tatggcatac acagatgaaa cctttaagac tcgtgaagct 1380
attcagcatg aatcaggaat cttgggacct ttactttatg gggaagttgg agacacactg 1440
ttgattatat ttaagaatca agcaagcaga ccatataaca tctaccctca cggaatcact 1500
gatgtccgtc ctttgtattc aaggagatta ccaaaaggtg taaaacattt gaaggatttt 1560
ccaattctgc caggagaaat attcaaatat aaatggacag tgactgtaga agatgggcca 1620
actaaatcag atcctcggtg cctgacccgc tattactcta gtttcgttaa tatggagaga 1680
gatctagctt caggactcat tggccctctc ctcatctgct acaaagaatc tgtagatcaa 1740
agaggaaacc agataatgtc agacaagagg aatgtcatcc tgttttctgt atttgatgag 1800
aaccgaagct ggtacctcac agagaatata caacgctttc tccccaatcc agctggagtg 1860
cagcttgagg atccagagtt ccaagcctcc aacatcatgc acagcatcaa tggctatgtt 1920
tttgatagtt tgcagttgtc agtttgtttg catgaggtgg catactggta cattctaagc 1980
attggagcac agactgactt cctttctgtc ttcttctctg gatatacctt caaacacaaa 2040
atggtctatg aagacacact caccctattc ccattctcag gagaaactgt cttcatgtcg 2100
atggaaaacc caggtctatg gattctggca tgccacaact cagactttcg gaacagaggc 2160
atgaccgcct tactgaaggt ttctagttgt gacaagaaca ctggtgatta ttacgaggac 2220
agttatgaag atatttcagc atacttgctg agtaaaaaca atgccattga accaagaagc 2280
ttctcccaga attcaagaca ccctagcact aggcaaaagc aatttaatgc caccacaatt 2340
ccagaaaatg acatagagaa gactgaccct tggtttgcac acagaacacc tatgcctaaa 2400
atacaaaatg tctcctctag tgatttgttg atgctcttgc gacagagtcc tactccacat 2460
gggctatcct tatctgatct ccaagaagcc aaatatgaga ctttttctga tgatccatca 2520
cctggagcaa tagacagtaa taacagcctg tctgaaatga cacacttcag gccacagctc 2580
catcacagtg gggacatggt atttacccct gagtcaggcc tccaattaag attaaatgag 2640
aaactgggga caactgcagc aacagagttg aagaaacttg atttcaaagt ttctagtaca 2700
tcaaataatc tgatttcaac aattccatca gacaatttgg cagcaggtac tgataataca 2760
agttccttag gacccccaag tatgccagtt cattatgata gtcaattaga taccactcta 2820
tttggcaaaa agtcatctcc ccttactgag tctggtggac ctctgagctt gagtgaagaa 2880
aataatgatt caaagttgtt agaatcaggt ttaatgaata gccaagaaag ttcatgggga 2940
aaaaatgtat cgtcaacaga gagtggtagg ttatttaaag ggaaaagagc tcatggacct 3000
gctttgttga ctaaagataa tgccttattc aaagttagca tctctttgtt aaagacaaac 3060
aaaacttcca ataattcagc aactaataga aagactcaca ttgatggccc atcattatta 3120
attgagaata gtccatcagt ctggcaaaat atattagaaa gtgacactga gtttaaaaaa 3180
gtgacacctt tgattcatga cagaatgctt atggacaaaa atgctacagc tttgaggcta 3240
aatcatatgt caaataaaac tacttcatca aaaaacatgg aaatggtcca acagaaaaaa 3300
gagggcccca ttccaccaga tgcacaaaat ccagatatgt cgttctttaa gatgctattc 3360
ttgccagaat cagcaaggtg gatacaaagg actcatggaa agaactctct gaactctggg 3420
caaggcccca gtccaaagca attagtatcc ttaggaccag aaaaatctgt ggaaggtcag 3480
aatttcttgt ctgagaaaaa caaagtggta gtaggaaagg gtgaatttac aaaggacgta 3540
ggactcaaag agatggtttt tccaagcagc agaaacctat ttcttactaa cttggataat 3600
ttacatgaaa ataatacaca caatcaagaa aaaaaaattc aggaagaaat agaaaagaag 3660
gaaacattaa tccaagagaa tgtagttttg cctcagatac atacagtgac tggcactaag 3720
aatttcatga agaacctttt cttactgagc actaggcaaa atgtagaagg ttcatatgac 3780
ggggcatatg ctccagtact tcaagatttt aggtcattaa atgattcaac aaatagaaca 3840
aagaaacaca cagctcattt ctcaaaaaaa ggggaggaag aaaacttgga aggcttggga 3900
aatcaaacca agcaaattgt agagaaatat gcatgcacca caaggatatc tcctaataca 3960
agccagcaga attttgtcac gcaacgtagt aagagagctt tgaaacaatt cagactccca 4020
ctagaagaaa cagaacttga aaaaaggata attgtggatg acacctcaac ccagtggtcc 4080
aaaaacatga aacatttgac cccgagcacc ctcacacaga tagactacaa tgagaaggag 4140
aaaggggcca ttactcagtc tcccttatca gattgcctta cgaggagtca tagcatccct 4200
caagcaaata gatctccatt acccattgca aaggtatcat catttccatc tattagacct 4260
atatatctga ccagggtcct attccaagac aactcttctc atcttccagc agcatcttat 4320
agaaagaaag attctggggt ccaagaaagc agtcatttct tacaaggagc caaaaaaaat 4380
aacctttctt tagccattct aaccttggag atgactggtg atcaaagaga ggttggctcc 4440
ctggggacaa gtgccacaaa ttcagtcaca tacaagaaag ttgagaacac tgttctcccg 4500
aaaccagact tgcccaaaac atctggcaaa gttgaattgc ttccaaaagt tcacatttat 4560
cagaaggacc tattccctac ggaaactagc aatgggtctc ctggccatct ggatctcgtg 4620
gaagggagcc ttcttcaggg aacagaggga gcgattaagt ggaatgaagc aaacagacct 4680
ggaaaagttc cctttctgag agtagcaaca gaaagctctg caaagactcc ctccaagcta 4740
ttggatcctc ttgcttggga taaccactat ggtactcaga taccaaaaga agagtggaaa 4800
tcccaagaga agtcaccaga aaaaacagct tttaagaaaa aggataccat tttgtccctg 4860
aacgcttgtg aaagcaatca tgcaatagca gcaataaatg agggacaaaa taagcccgaa 4920
atagaagtca cctgggcaaa gcaaggtagg actgaaaggc tgtgctctca aaacccacca 4980
gtcttgaaac gccatcaacg ggaaataact cgtactactc ttcagtcaga tcaagaggaa 5040
attgactatg atgataccat atcagttgaa atgaagaagg aagattttga catttatgat 5100
gaggatgaaa atcagagccc ccgcagcttt caaaagaaaa cacgacacta ttttattgct 5160
gcagtggaga ggctctggga ttatgggatg agtagctccc cacatgttct aagaaacagg 5220
gctcagagtg gcagtgtccc tcagttcaag aaagttgttt tccaggaatt tactgatggc 5280
tcctttactc agcccttata ccgtggagaa ctaaatgaac atttgggact cctggggcca 5340
tatataagag cagaagttga agataatatc atggtaactt tcagaaatca ggcctctcgt 5400
ccctattcct tctattctag ccttatttct tatgaggaag atcagaggca aggagcagaa 5460
cctagaaaaa actttgtcaa gcctaatgaa accaaaactt acttttggaa agtgcaacat 5520
catatggcac ccactaaaga tgagtttgac tgcaaagcct gggcttattt ctctgatgtt 5580
gacctggaaa aagatgtgca ctcaggcctg attggacccc ttctggtctg ccacactaac 5640
acactgaacc ctgctcatgg gagacaagtg acagtacagg aatttgctct gtttttcacc 5700
atctttgatg agaccaaaag ctggtacttc actgaaaata tggaaagaaa ctgcagggct 5760
ccctgcaata tccagatgga agatcccact tttaaagaga attatcgctt ccatgcaatc 5820
aatggctaca taatggatac actacctggc ttagtaatgg ctcaggatca aaggattcga 5880
tggtatctgc tcagcatggg cagcaatgaa aacatccatt ctattcattt cagtggacat 5940
gtgttcactg tacgaaaaaa agaggagtat aaaatggcac tgtacaatct ctatccaggt 6000
gtttttgaga cagtggaaat gttaccatcc aaagctggaa tttggcgggt ggaatgcctt 6060
attggcgagc atctacatgc tgggatgagc acactttttc tggtgtacag caataagtgt 6120
cagactcccc tgggaatggc ttctggacac attagagatt ttcagattac agcttcagga 6180
caatatggac agtgggcccc aaagctggcc agacttcatt attccggatc aatcaatgcc 6240
tggagcacca aggagccctt ttcttggatc aaggtggatc tgttggcacc aatgattatt 6300
cacggcatca agacccaggg tgcccgtcag aagttctcca gcctctacat ctctcagttt 6360
atcatcatgt atagtcttga tgggaagaag tggcagactt atcgaggaaa ttccactgga 6420
accttaatgg tcttctttgg caatgtggat tcatctggga taaaacacaa tatttttaac 6480
cctccaatta ttgctcgata catccgtttg cacccaactc attatagcat tcgcagcact 6540
cttcgcatgg agttgatggg ctgtgattta aatagttgca gcatgccatt gggaatggag 6600
agtaaagcaa tatcagatgc acagattact gcttcatcct actttaccaa tatgtttgcc 6660
acctggtctc cttcaaaagc tcgacttcac ctccaaggga ggagtaatgc ctggagacct 6720
caggtgaata atccaaaaga gtggctgcaa gtggacttcc agaagacaat gaaagtcaca 6780
ggagtaacta ctcagggagt aaaatctctg cttaccagca tgtatgtgaa ggagttcctc 6840
atctccagca gtcaagatgg ccatcagtgg actctctttt ttcagaatgg caaagtaaag 6900
gtttttcagg gaaatcaaga ctccttcaca cctgtggtga actctctaga cccaccgtta 6960
ctgactcgct accttcgaat tcacccccag agttgggtgc accagattgc cctgaggatg 7020
gaggttctgg gctgcgaggc acaggacctc tactag 7056
<210> 4
<211> 7056
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 4
atgcaaatag agctctccac ctgcttcttt ctgtgccttt tgcgattctg ctttagtgcc 60
accagaagat actacctggg tgcagtggaa ctgtcatggg actatatgca aagtgatctc 120
ggtgagctgc ctgtggacgc aagatttcct cctagagtgc caaaatcttt tccattcaac 180
acctcagtcg tgtacaaaaa gactctgttt gtagaattca cggatcacct tttcaacatc 240
gctaagccaa ggccaccctg gatgggtctg ctaggtccta ccatccaggc tgaggtttat 300
gatacagtgg tcattacact taagaacatg gcttcccatc ctgtcagtct tcatgctgtt 360
ggtgtatcct actggaaagc ttctgaggga gctgaatatg atgatcagac cagtcaaagg 420
gagaaagaag atgataaagt cttccctggt ggaagccata catatgtctg gcaggtcctg 480
aaagagaatg gtccaatggc ctctgaccca ctgtgcctta cctactcata tctttctcat 540
gtggacctgg taaaagactt gaattcaggc ctcattggag ccctactagt atgtagagaa 600
gggagtctgg ccaaggaaaa gacacagacc ttgcacaaat ttatactact ttttgctgta 660
tttgatgaag ggaaaagttg gcactcagaa acaaagaact ccttgatgca ggatagggat 720
gctgcatctg ctcgggcctg gcctaaaatg cacacagtca atggttatgt aaacaggtct 780
ctgccaggtc tgattggatg ccacaggaaa tcagtctatt ggcatgtgat tggaatgggc 840
accactcctg aagtgcactc aatattcctc gaaggtcaca catttcttgt gaggaaccat 900
cgccaggcgt ccttggaaat ctcgccaata actttcctta ctgctcaaac actcttgatg 960
gaccttggac agtttctact gttttgtcat atctcttccc accaacatga tggcatggaa 1020
gcttatgtca aagtagacag ctgtccagag gaaccccaac tacgaatgaa aaataatgaa 1080
gaagcggaag actatgatga tgatcttact gattctgaaa tggatgtggt caggtttgat 1140
gatgacaact ctccttcctt tatccaaatt cgctcagttg ccaagaagca tcctaaaact 1200
tgggtacatt acattgctgc tgaagaggag gactgggact atgctccctt agtcctcgcc 1260
cccgatgaca gaagttataa aagtcaatat ttgaacaatg gccctcagcg gattggtagg 1320
aagtacaaaa aagtccgatt tatggcatac acagatgaaa cctttaagac tcgtgaagct 1380
attcagcatg aatcaggaat cttgggacct ttactttatg gggaagttgg agacacactg 1440
ttgattatat ttaagaatca agcaagcaga ccatataaca tctaccctca cggaatcact 1500
gatgtccgtc ctttgtattc aaggagatta ccaaaaggtg taaaacattt gaaggatttt 1560
ccaattctgc caggagaaat attcaaatat aaatggacag tgactgtaga agatgggcca 1620
actaaatcag atcctcggtg cctgacccgc tattactcta gtttcgttaa tatggagaga 1680
gatctagctt caggactcat tggccctctc ctcatctgct acaaagaatc tgtagatcaa 1740
agaggaaacc agataatgtc agacaagagg aatgtcatcc tgttttctgt atttgatgag 1800
aaccgaagct ggtacctcac agagaatata caacgctttc tccccaatcc agctggagtg 1860
cagcttgagg atccagagtt ccaagcctcc aacatcatgc acagcatcaa tggctatgtt 1920
tttgatagtt tgcagttgtc agtttgtttg catgaggtgg catactggta cattctaagc 1980
attggagcac agactgactt cctttctgtc ttcttctctg gatatacctt caaacacaaa 2040
atggtctatg aagacacact caccctattc ccattctcag gagaaactgt cttcatgtcg 2100
atggaaaacc caggtctatg gattctggcg tgccacaact cagactttcg gaacagaggc 2160
atgaccgcct tactgaaggt ttctagttgt gacaagaaca ctggtgatta ttacgaggac 2220
agttatgaag atatttcagc atacttgctg agtaaaaaca atgccattga accaagaagc 2280
ttctcccaga attcaagaca ccctagcact aggcaaaagc aatttaatgc caccacaatt 2340
ccagaaaatg acatagagaa gactgaccct tggtttgcac acagaacacc tatgcctaaa 2400
atacaaaatg tctcctctag tgatttgttg atgctcttgc gacagagtcc tactccacat 2460
gggctatcct tatctgatct ccaagaagcc aaatatgaga ctttttctga tgatccatca 2520
cctggagcaa tagacagtaa taacagcctg tctgaaatga cacacttcag gccacagctc 2580
catcacagtg gggacatggt atttacccct gagtcaggcc tccaattaag attaaatgag 2640
aaactgggga caactgcagc aacagagttg aagaaacttg atttcaaagt ttctagtaca 2700
tcaaataatc tgatttcaac aattccatca gacaatttgg cagcaggtac tgataataca 2760
agttccttag gacccccaag tatgccagtt cattatgata gtcaattaga taccactcta 2820
tttggcaaaa agtcatctcc ccttactgag tctggtggac ctctgagctt gagtgaagaa 2880
aataatgatt caaagttgtt agaatcaggt ttaatgaata gccaagaaag ttcatgggga 2940
aaaaatgtat cgtcaacaga gagtggtagg ttatttaaag ggaaaagagc tcatggacct 3000
gctttgttga ctaaagataa tgccttattc aaagttagca tctctttgtt aaagacaaac 3060
aaaacttcca ataattcagc aactaataga aagactcaca ttgatggccc atcattatta 3120
attgagaata gtccatcagt ctggcaaaat atattagaaa gtgacactga gtttaaaaaa 3180
gtgacacctt tgattcatga cagaatgctt atggacaaaa atgctacagc tttgaggcta 3240
aatcatatgt caaataaaac tacttcatca aaaaacatgg aaatggtcca acagaaaaaa 3300
gagggcccca ttccaccaga tgcacaaaat ccagatatgt cgttctttaa gatgctattc 3360
ttgccagaat cagcaaggtg gatacaaagg actcatggaa agaactctct gaactctggg 3420
caaggcccca gtccaaagca attagtatcc ttaggaccag aaaaatctgt ggaaggtcag 3480
aatttcttgt ctgagaaaaa caaagtggta gtaggaaagg gtgaatttac aaaggacgta 3540
ggactcaaag agatggtttt tccaagcagc agaaacctat ttcttactaa cttggataat 3600
ttacatgaaa ataatacaca caatcaagaa aaaaaaattc aggaagaaat agaaaagaag 3660
gaaacattaa tccaagagaa tgtagttttg cctcagatac atacagtgac tggcactaag 3720
aatttcatga agaacctttt cttactgagc actaggcaaa atgtagaagg ttcatatgac 3780
ggggcatatg ctccagtact tcaagatttt aggtcattaa atgattcaac aaatagaaca 3840
aagaaacaca cagctcattt ctcaaaaaaa ggggaggaag aaaacttgga aggcttggga 3900
aatcaaacca agcaaattgt agagaaatat gcatgcacca caaggatatc tcctaataca 3960
agccagcaga attttgtcac gcaacgtagt aagagagctt tgaaacaatt cagactccca 4020
ctagaagaaa cagaacttga aaaaaggata attgtggatg acacctcaac ccagtggtcc 4080
aaaaacatga aacatttgac cccgagcacc ctcacacaga tagactacaa tgagaaggag 4140
aaaggggcca ttactcagtc tcccttatca gattgcctta cgaggagtca tagcatccct 4200
caagcaaata gatctccatt acccattgca aaggtatcat catttccatc tattagacct 4260
atatatctga ccagggtcct attccaagac aactcttctc atcttccagc agcatcttat 4320
agaaagaaag attctggggt ccaagaaagc agtcatttct tacaaggagc caaaaaaaat 4380
aacctttctt tagccattct aaccttggag atgactggtg atcaaagaga ggttggctcc 4440
ctggggacaa gtgccacaaa ttcagtcaca tacaagaaag ttgagaacac tgttctcccg 4500
aaaccagact tgcccaaaac atctggcaaa gttgaattgc ttccaaaagt tcacatttat 4560
cagaaggacc tattccctac ggaaactagc aatgggtctc ctggccatct ggatctcgtg 4620
gaagggagcc ttcttcaggg aacagaggga gcgattaagt ggaatgaagc aaacagacct 4680
ggaaaagttc cctttctgag agtagcaaca gaaagctctg caaagactcc ctccaagcta 4740
ttggatcctc ttgcttggga taaccactat ggtactcaga taccaaaaga agagtggaaa 4800
tcccaagaga agtcaccaga aaaaacagct tttaagaaaa aggataccat tttgtccctg 4860
aacgcttgtg aaagcaatca tgcaatagca gcaataaatg agggacaaaa taagcccgaa 4920
atagaagtca cctgggcaaa gcaaggtagg actgaaaggc tgtgctctca aaacccacca 4980
gtcttgaaac gccatcaacg ggaaataact cgtactactc ttcagtcaga tcaagaggaa 5040
attgactatg atgataccat atcagttgaa atgaagaagg aagattttga catttatgat 5100
gaggatgaaa atcagagccc ccgcagcttt caaaagaaaa cacgacacta ttttattgct 5160
gcagtggaga ggctctggga ttatgggatg agtagctccc cacatgttct aagaaacagg 5220
gctcagagtg gcagtgtccc tcagttcaag aaagttgttt tccaggaatt tactgatggc 5280
tcctttactc agcccttata ccgtggagaa ctaaatgaac atttgggact cctggggcca 5340
tatataagag cagaagttga agataatatc atggtaactt tcagaaatca ggcctctcgt 5400
ccctattcct tctattctag ccttatttct tatgaggaag atcagaggca aggagcagaa 5460
cctagaaaaa actttgtcaa gcctaatgaa accaaaactt acttttggaa agtgcaacat 5520
catatggcac ccactaaaga tgagtttgac tgcaaagcct gggcttattt ctctgatgtt 5580
gacctggaaa aagatgtgca ctcaggcctg attggacccc ttctggtctg ccacactaac 5640
acactgaacc ctgctcatgg gagacaagtg acagtacagg aatttgctct gtttttcacc 5700
atctttgatg agaccaaaag ctggtacttc actgaaaata tggaaagaaa ctgcagggct 5760
ccctgcaata tccagatgga agatcccact tttaaagaga attatcgctt ccatgcaatc 5820
aatggctaca taatggatac actacctggc ttagtaatgg ctcaggatca aaggattcga 5880
tggtatctgc tcagcatggg cagcaatgaa aacatccatt ctattcattt cagtggacat 5940
gtgttcactg tacgaaaaaa agaggagtat aaaatggcac tgtacaatct ctatccaggt 6000
gtttttgaga cagtggaaat gttaccatcc aaagctggaa tttggcgggt ggaatgcctt 6060
attggcgagc atctacatgc tgggatgagc acactttttc tggtgtacag caataagtgt 6120
cagactcccc tgggaatggc ttctggacac attagagatt ttcagattac agcttcagga 6180
caatatggac agtgggcccc aaagctggcc agacttcatt attccggatc aatcaatgcc 6240
tggagcacca aggagccctt ttcttggatc aaggtggatc tgttggcacc aatgattatt 6300
cacggcatca agacccaggg tgcccgtcag aagttctcca gcctctacat ctctcagttt 6360
atcatcatgt atagtcttga tgggaagaag tggcagactt atcgaggaaa ttccactgga 6420
accttaatgg tcttctttgg caatgtggat tcatctggga taaaacacaa tatttttaac 6480
cctccaatta ttgctcgata catccgtttg cacccaactc attatagcat tcgcagcact 6540
cttcgcatgg agttgatggg ctgtgattta aatagttgca gcatgccatt gggaatggag 6600
agtaaagcaa tatcagatgc acagattact gcttcatcct actttaccaa tatgtttgcc 6660
acctggtctc cttcaaaagc tcgacttcac ctccaaggga ggagtaatgc ctggagacct 6720
caggtgaata atccaaaaga gtggctgcaa gtggacttcc agaagacaat gaaagtcaca 6780
ggagtaacta ctcagggagt aaaatctctg cttaccagca tgtatgtgaa ggagttcctc 6840
atctccagca gtcaagatgg ccatcagtgg actctctttt ttcagaatgg caaagtaaag 6900
gtttttcagg gaaatcaaga ctccttcaca cctgtggtga actctctaga cccaccgtta 6960
ctgactcgct accttcgaat tcacccccag agttgggtgc accagattgc cctgaggatg 7020
gaggttctgg gctgcgaggc acaggacctc tactag 7056
<210> 6
<211> 4374
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 6
atgcagatcg aactgagcac ttgcttcttc ctgtgtctcc tgcgcttttg cttctccgcc 60
acaaggagat actatctcgg tgccgtggag ctcagctggg actacatgca gagcgacttg 120
ggtgaactgc ctgtggacgc caggtttcca ccccgcgtgc ccaagagttt cccgttcaac 180
accagtgtcg tgtacaagaa aaccctcttc gtggaattca ccgaccacct gttcaacatc 240
gccaaaccgc gccctccctg gatggggctg ctcggcccga cgatccaggc tgaggtctat 300
gacacggtgg tgattaccct caagaacatg gctagccacc cggtgagcct gcacgccgtg 360
ggcgtgtcct attggaaagc gtccgagggt gcggagtacg atgaccagac ttcacagcgg 420
gagaaggaag acgacaaagt gttccccggg ggttcccaca cctatgtctg gcaggtcctg 480
aaggagaatg gtcctatggc ctccgaccca ttgtgcctca cctactctta cctaagccat 540
gtggatctcg tcaaggacct gaactcgggg ctgatcggcg ccctgctcgt gtgccgggag 600
ggctcactgg ccaaggagaa gacccaaact ctgcacaagt tcatcctgct gttcgcggta 660
ttcgacgagg ggaagtcctg gcactccgag accaagaaca gcctgatgca ggaccgcgac 720
gcagcctcgg cccgtgcgtg gccaaagatg cacaccgtga acggctacgt taacaggagc 780
ctacccggcc tgatcggctg ccaccgcaaa tcggtctact ggcatgtgat cggaatgggc 840
acaacgcccg aggtccacag tatcttcctc gagggccaca ctttcctggt ccggaatcac 900
cgccaggcca gcctggagat cagccccata acctttctga cggcgcagac cttactcatg 960
gatctcggcc agttcctcct gttctgccac atttcgtccc accagcacga tgggatggaa 1020
gcatatgtga aagtggactc ctgccccgag gaaccccagc ttaggatgaa gaacaatgag 1080
gaggccgagg actacgacga tgaccttacc gattcagaaa tggacgtagt acgctttgac 1140
gacgacaact ctccatcctt catacagatt cgctccgtcg ccaagaagca ccctaagact 1200
tgggtgcact acatcgcggc cgaggaggag gactgggatt atgctcccct ggtgctggcc 1260
cccgacgacc gcagctacaa gagccagtac ctgaataacg ggccccagcg catcggccgg 1320
aagtacaaga aagtgcggtt catggcttac acggacgaga ccttcaagac ccgggaggct 1380
atccagcatg agagcggcat cttggggccc ctcctgtacg gcgaagttgg agacacactg 1440
ctgatcatct tcaagaacca ggcgagcagg ccctacaaca tctaccccca cggcattacc 1500
gatgtccggc cgttgtacag ccgacggctg cccaagggcg tgaagcacct gaaggacttt 1560
ccgatcctgc cgggcgagat cttcaagtac aagtggactg tgaccgtgga ggatgggccg 1620
accaagagcg atccgcgctg cctgacccgt tactactcca gctttgtcaa tatggagcgc 1680
gacctcgcta gcggcttgat tggccctctg ctgatctgct acaaggagtc cgtggaccag 1740
agggggaatc agatcatgag tgacaagagg aacgtgatcc tgttctccgt gttcgacgaa 1800
aaccgcagct ggtatctcac cgagaatatc cagcgcttcc tgcccaaccc ggccggtgtg 1860
cagctggagg accccgagtt tcaggccagc aacatcatgc attctatcaa cggatatgtg 1920
tttgattccc tgcagctctc agtgtgtctg cacgaggtcg cctactggta tatcctcagc 1980
attggggcac agaccgactt cctgagcgtg ttcttctccg ggtatacctt caagcacaag 2040
atggtgtacg aggataccct gaccctgttc ccctttagcg gcgaaaccgt gtttatgtct 2100
atggagaacc ccgggctctg gatccttgcc tgccataact ccgacttccg caaccgcgga 2160
atgaccgcgc tcctgaaagt gtcgagttgt gacaagaaca ccggcgacta ttacgaggac 2220
agttacgagg acatctctgc gtacctcctt agcaagaata acgccatcga gccaagatcc 2280
ttcagccaga accccccagt gctgaagagg catcagcggg agatcacccg cacgaccctg 2340
cagtcggatc aggaggagat tgattacgac gacacgatca gtgtggagat gaagaaggag 2400
gacttcgaca tctacgacga agatgaaaac cagtcccctc ggtccttcca aaagaagacc 2460
cggcactact tcatcgccgc tgtggaacgc ctgtgggact atggaatgtc ttctagccct 2520
cacgttttga ggaaccgcgc ccagtcgggc agcgtgcccc agttcaagaa agtggtgttc 2580
caggagttca ccgacggctc cttcacccag ccactttacc ggggcgagct caatgaacat 2640
ctgggcctgc tgggacccta catcagggct gaggtggagg acaacatcat ggtgacattc 2700
cggaatcagg ccagcagacc atacagtttc tacagttcac tcatctccta cgaggaggac 2760
cagcgccagg gggctgaacc ccgtaagaac ttcgtgaagc caaacgaaac aaagacctac 2820
ttctggaagg tccagcacca catggcacct accaaggacg agttcgattg caaggcctgg 2880
gcctacttct ccgacgtgga cctggagaaa gatgtgcaca gcggcctgat tggccctctg 2940
ctggtgtgtc acacgaacac actcaaccct gcacacgggc ggcaggtcac tgtgcaggaa 3000
ttcgccctgt tctttaccat ctttgatgag acgaagtcct ggtatttcac cgaaaacatg 3060
gagaggaact gccgcgcacc ctgcaacatc cagatggaag atccgacatt caaggagaac 3120
taccggttcc atgccatcaa tggctacatc atggacaccc tgcctggcct cgtgatggcc 3180
caagaccagc gtatccgctg gtatctgctg tcgatgggct ccaacgagaa catccatagt 3240
atccacttca gcgggcatgt cttcacggtg aggaaaaagg aggagtacaa gatggcactg 3300
tacaacctct atcccggcgt gttcgagacc gtggagatgc tgccctccaa ggccggcatc 3360
tggagagtgg aatgcctgat cggcgagcac ctccacgctg ggatgtccac gctgttcctc 3420
gtttacagca ataagtgcca gacccctctg ggcatggcga gcggccacat ccgcgacttc 3480
cagattacag ccagcggcca gtacggtcag tgggctccaa agctggcccg tctgcactac 3540
tccggatcca tcaacgcctg gtccaccaag gaaccgttct cctggatcaa agtagacctg 3600
ctagccccca tgatcattca cggcatcaag acacaaggcg cccgacagaa gttctcgagc 3660
ctctatatct cccagttcat catcatgtat agcctggacg gaaagaagtg gcagacttac 3720
cgcggaaact cgacagggac cctgatggta ttcttcggta acgtggacag ctccggaatc 3780
aagcacaaca tcttcaaccc acccattatc gcccgctaca tccgcctgca ccccactcac 3840
tatagcatta ggtccaccct gcgaatggag ctcatgggct gtgacctgaa cagctgtagc 3900
atgcccctcg gcatggagtc taaggcgatc tccgacgcac agataacggc atcatcctac 3960
tttaccaaca tgttcgctac ctggtccccc tccaaggccc gactccacct gcaagggaga 4020
tccaacgcct ggcggccaca ggtcaacaat cccaaggagt ggctgcaagt ggactttcag 4080
aaaactatga aagtcaccgg agtgaccaca cagggagtga agtctctgct gaccagcatg 4140
tacgtgaagg agttcctcat ctccagttcg caggatggcc accagtggac gttgttcttc 4200
caaaacggta aagtcaaagt cttccaaggg aaccaggaca gctttacacc cgtcgtgaac 4260
tccctggacc ccccgcttct cactagatac ctccgcatcc accctcagag ctgggtgcac 4320
cagattgccc tgcgcatgga ggttctgggg tgtgaagccc aggacctgta ctaa 4374
<210> 5
<211> 4374
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 5
atgcagatcg aactgagcac ttgcttcttc ctgtgtctcc tgcgcttttg cttctccgcc 60
acaaggagat actatctcgg tgccgtggag ctcagctggg actacatgca gagcgacttg 120
ggtgaactgc ctgtggacgc caggtttcca ccccgcgtgc ccaagagttt cccgttcaac 180
accagtgtcg tgtacaagaa aaccctcttc gtggaattca ccgaccacct gttcaacatc 240
gccaaaccgc gccctccctg gatggggctg ctcggcccga cgatccaggc tgaggtctat 300
gacacggtgg tgattaccct caagaacatg gctagccacc cggtgagcct gcacgccgtg 360
ggcgtgtcct attggaaagc gtccgagggt gcggagtacg atgaccagac ttcacagcgg 420
gagaaggaag acgacaaagt gttccccggg ggttcccaca cctatgtctg gcaggtcctg 480
aaggagaatg gtcctatggc ctccgaccca ttgtgcctca cctactctta cctaagccat 540
gtggatctcg tcaaggacct gaactcgggg ctgatcggcg ccctgctcgt gtgccgggag 600
ggctcactgg ccaaggagaa gacccaaact ctgcacaagt tcatcctgct gttcgcggta 660
ttcgacgagg ggaagtcctg gcactccgag accaagaaca gcctgatgca ggaccgcgac 720
gcagcctcgg cccgtgcgtg gccaaagatg cacaccgtga acggctacgt taacaggagc 780
ctacccggcc tgatcggctg ccaccgcaaa tcggtctact ggcatgtgat cggaatgggc 840
acaacgcccg aggtccacag tatcttcctc gagggccaca ctttcctggt ccggaatcac 900
cgccaggcca gcctggagat cagccccata acctttctga cggcgcagac cttactcatg 960
gatctcggcc agttcctcct gttctgccac atttcgtccc accagcacga tgggatggaa 1020
gcatatgtga aagtggactc ctgccccgag gaaccccagc ttaggatgaa gaacaatgag 1080
gaggccgagg actacgacga tgaccttacc gattcagaaa tggacgtagt acgctttgac 1140
gacgacaact ctccatcctt catacagatt cgctccgtcg ccaagaagca ccctaagact 1200
tgggtgcact acatcgcggc cgaggaggag gactgggatt atgctcccct ggtgctggcc 1260
cccgacgacc gcagctacaa gagccagtac ctgaataacg ggccccagcg catcggccgg 1320
aagtacaaga aagtgcggtt catggcttac acggacgaga ccttcaagac ccgggaggct 1380
atccagcatg agagcggcat cttggggccc ctcctgtacg gcgaagttgg agacacactg 1440
ctgatcatct tcaagaacca ggcgagcagg ccctacaaca tctaccccca cggcattacc 1500
gatgtccggc cgttgtacag ccgacggctg cccaagggcg tgaagcacct gaaggacttt 1560
ccgatcctgc cgggcgagat cttcaagtac aagtggactg tgaccgtgga ggatgggccg 1620
accaagagcg atccgcgctg cctgacccgt tactactcca gctttgtcaa tatggagcgc 1680
gacctcgcta gcggcttgat tggccctctg ctgatctgct acaaggagtc cgtggaccag 1740
agggggaatc agatcatgag tgacaagagg aacgtgatcc tgttctccgt gttcgacgaa 1800
aaccgcagct ggtatctcac cgagaatatc cagcgcttcc tgcccaaccc ggccggtgtg 1860
cagctggagg accccgagtt tcaggccagc aacatcatgc attctatcaa cggatatgtg 1920
tttgattccc tgcagctctc agtgtgtctg cacgaggtcg cctactggta tatcctcagc 1980
attggggcac agaccgactt cctgagcgtg ttcttctccg ggtatacctt caagcacaag 2040
atggtgtacg aggataccct gaccctgttc ccctttagcg gcgaaaccgt gtttatgtct 2100
atggagaacc ccgggctctg gatccttgct tgccataact ccgacttccg caaccgcgga 2160
atgaccgcgc tcctgaaagt gtcgagttgt gacaagaaca ccggcgacta ttacgaggac 2220
agttacgagg acatctctgc gtacctcctt agcaagaata acgccatcga gccaagatcc 2280
ttcagccaga accccccagt gctgaagagg catcagcggg agatcacccg cacgaccctg 2340
cagtcggatc aggaggagat tgattacgac gacacgatca gtgtggagat gaagaaggag 2400
gacttcgaca tctacgacga agatgaaaac cagtcccctc ggtccttcca aaagaagacc 2460
cggcactact tcatcgccgc tgtggaacgc ctgtgggact atggaatgtc ttctagccct 2520
cacgttttga ggaaccgcgc ccagtcgggc agcgtgcccc agttcaagaa agtggtgttc 2580
caggagttca ccgacggctc cttcacccag ccactttacc ggggcgagct caatgaacat 2640
ctgggcctgc tgggacccta catcagggct gaggtggagg acaacatcat ggtgacattc 2700
cggaatcagg ccagcagacc atacagtttc tacagttcac tcatctccta cgaggaggac 2760
cagcgccagg gggctgaacc ccgtaagaac ttcgtgaagc caaacgaaac aaagacctac 2820
ttctggaagg tccagcacca catggcacct accaaggacg agttcgattg caaggcctgg 2880
gcctacttct ccgacgtgga cctggagaaa gatgtgcaca gcggcctgat tggccctctg 2940
ctggtgtgtc acacgaacac actcaaccct gcacacgggc ggcaggtcac tgtgcaggaa 3000
ttcgccctgt tctttaccat ctttgatgag acgaagtcct ggtatttcac cgaaaacatg 3060
gagaggaact gccgcgcacc ctgcaacatc cagatggaag atccgacatt caaggagaac 3120
taccggttcc atgccatcaa tggctacatc atggacaccc tgcctggcct cgtgatggcc 3180
caagaccagc gtatccgctg gtatctgctg tcgatgggct ccaacgagaa catccatagt 3240
atccacttca gcgggcatgt cttcacggtg aggaaaaagg aggagtacaa gatggcactg 3300
tacaacctct atcccggcgt gttcgagacc gtggagatgc tgccctccaa ggccggcatc 3360
tggagagtgg aatgcctgat cggcgagcac ctccacgctg ggatgtccac gctgttcctc 3420
gtttacagca ataagtgcca gacccctctg ggcatggcga gcggccacat ccgcgacttc 3480
cagattacag ccagcggcca gtacggtcag tgggctccaa agctggcccg tctgcactac 3540
tccggatcca tcaacgcctg gtccaccaag gaaccgttct cctggatcaa agtagacctg 3600
ctagccccca tgatcattca cggcatcaag acacaaggcg cccgacagaa gttctcgagc 3660
ctctatatct cccagttcat catcatgtat agcctggacg gaaagaagtg gcagacttac 3720
cgcggaaact cgacagggac cctgatggta ttcttcggta acgtggacag ctccggaatc 3780
aagcacaaca tcttcaaccc acccattatc gcccgctaca tccgcctgca ccccactcac 3840
tatagcatta ggtccaccct gcgaatggag ctcatgggct gtgacctgaa cagctgtagc 3900
atgcccctcg gcatggagtc taaggcgatc tccgacgcac agataacggc atcatcctac 3960
tttaccaaca tgttcgctac ctggtccccc tccaaggccc gactccacct gcaagggaga 4020
tccaacgcct ggcggccaca ggtcaacaat cccaaggagt ggctgcaagt ggactttcag 4080
aaaactatga aagtcaccgg agtgaccaca cagggagtga agtctctgct gaccagcatg 4140
tacgtgaagg agttcctcat ctccagttcg caggatggcc accagtggac gttgttcttc 4200
caaaacggta aagtcaaagt cttccaaggg aaccaggaca gctttacacc cgtcgtgaac 4260
tccctggacc ccccgcttct cactagatac ctccgcatcc accctcagag ctgggtgcac 4320
cagattgccc tgcgcatgga ggttctgggg tgtgaagccc aggacctgta ctaa 4374
<210> 7
<211> 4374
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 7
atgcagatcg aactgagcac ttgcttcttc ctgtgtctcc tgcgcttttg cttctccgcc 60
acaaggagat actatctcgg tgccgtggag ctcagctggg actacatgca gagcgacttg 120
ggtgaactgc ctgtggacgc caggtttcca ccccgcgtgc ccaagagttt cccgttcaac 180
accagtgtcg tgtacaagaa aaccctcttc gtggaattca ccgaccacct gttcaacatc 240
gccaaaccgc gccctccctg gatggggctg ctcggcccga cgatccaggc tgaggtctat 300
gacacggtgg tgattaccct caagaacatg gctagccacc cggtgagcct gcacgccgtg 360
ggcgtgtcct attggaaagc gtccgagggt gcggagtacg atgaccagac ttcacagcgg 420
gagaaggaag acgacaaagt gttccccggg ggttcccaca cctatgtctg gcaggtcctg 480
aaggagaatg gtcctatggc ctccgaccca ttgtgcctca cctactctta cctaagccat 540
gtggatctcg tcaaggacct gaactcgggg ctgatcggcg ccctgctcgt gtgccgggag 600
ggctcactgg ccaaggagaa gacccaaact ctgcacaagt tcatcctgct gttcgcggta 660
ttcgacgagg ggaagtcctg gcactccgag accaagaaca gcctgatgca ggaccgcgac 720
gcagcctcgg cccgtgcgtg gccaaagatg cacaccgtga acggctacgt taacaggagc 780
ctacccggcc tgatcggctg ccaccgcaaa tcggtctact ggcatgtgat cggaatgggc 840
acaacgcccg aggtccacag tatcttcctc gagggccaca ctttcctggt ccggaatcac 900
cgccaggcca gcctggagat cagccccata acctttctga cggcgcagac cttactcatg 960
gatctcggcc agttcctcct gttctgccac atttcgtccc accagcacga tgggatggaa 1020
gcatatgtga aagtggactc ctgccccgag gaaccccagc ttaggatgaa gaacaatgag 1080
gaggccgagg actacgacga tgaccttacc gattcagaaa tggacgtagt acgctttgac 1140
gacgacaact ctccatcctt catacagatt cgctccgtcg ccaagaagca ccctaagact 1200
tgggtgcact acatcgcggc cgaggaggag gactgggatt atgctcccct ggtgctggcc 1260
cccgacgacc gcagctacaa gagccagtac ctgaataacg ggccccagcg catcggccgg 1320
aagtacaaga aagtgcggtt catggcttac acggacgaga ccttcaagac ccgggaggct 1380
atccagcatg agagcggcat cttggggccc ctcctgtacg gcgaagttgg agacacactg 1440
ctgatcatct tcaagaacca ggcgagcagg ccctacaaca tctaccccca cggcattacc 1500
gatgtccggc cgttgtacag ccgacggctg cccaagggcg tgaagcacct gaaggacttt 1560
ccgatcctgc cgggcgagat cttcaagtac aagtggactg tgaccgtgga ggatgggccg 1620
accaagagcg atccgcgctg cctgacccgt tactactcca gctttgtcaa tatggagcgc 1680
gacctcgcta gcggcttgat tggccctctg ctgatctgct acaaggagtc cgtggaccag 1740
agggggaatc agatcatgag tgacaagagg aacgtgatcc tgttctccgt gttcgacgaa 1800
aaccgcagct ggtatctcac cgagaatatc cagcgcttcc tgcccaaccc ggccggtgtg 1860
cagctggagg accccgagtt tcaggccagc aacatcatgc attctatcaa cggatatgtg 1920
tttgattccc tgcagctctc agtgtgtctg cacgaggtcg cctactggta tatcctcagc 1980
attggggcac agaccgactt cctgagcgtg ttcttctccg ggtatacctt caagcacaag 2040
atggtgtacg aggataccct gaccctgttc ccctttagcg gcgaaaccgt gtttatgtct 2100
atggagaacc ccgggctctg gatccttgca tgccataact ccgacttccg caaccgcgga 2160
atgaccgcgc tcctgaaagt gtcgagttgt gacaagaaca ccggcgacta ttacgaggac 2220
agttacgagg acatctctgc gtacctcctt agcaagaata acgccatcga gccaagatcc 2280
ttcagccaga accccccagt gctgaagagg catcagcggg agatcacccg cacgaccctg 2340
cagtcggatc aggaggagat tgattacgac gacacgatca gtgtggagat gaagaaggag 2400
gacttcgaca tctacgacga agatgaaaac cagtcccctc ggtccttcca aaagaagacc 2460
cggcactact tcatcgccgc tgtggaacgc ctgtgggact atggaatgtc ttctagccct 2520
cacgttttga ggaaccgcgc ccagtcgggc agcgtgcccc agttcaagaa agtggtgttc 2580
caggagttca ccgacggctc cttcacccag ccactttacc ggggcgagct caatgaacat 2640
ctgggcctgc tgggacccta catcagggct gaggtggagg acaacatcat ggtgacattc 2700
cggaatcagg ccagcagacc atacagtttc tacagttcac tcatctccta cgaggaggac 2760
cagcgccagg gggctgaacc ccgtaagaac ttcgtgaagc caaacgaaac aaagacctac 2820
ttctggaagg tccagcacca catggcacct accaaggacg agttcgattg caaggcctgg 2880
gcctacttct ccgacgtgga cctggagaaa gatgtgcaca gcggcctgat tggccctctg 2940
ctggtgtgtc acacgaacac actcaaccct gcacacgggc ggcaggtcac tgtgcaggaa 3000
ttcgccctgt tctttaccat ctttgatgag acgaagtcct ggtatttcac cgaaaacatg 3060
gagaggaact gccgcgcacc ctgcaacatc cagatggaag atccgacatt caaggagaac 3120
taccggttcc atgccatcaa tggctacatc atggacaccc tgcctggcct cgtgatggcc 3180
caagaccagc gtatccgctg gtatctgctg tcgatgggct ccaacgagaa catccatagt 3240
atccacttca gcgggcatgt cttcacggtg aggaaaaagg aggagtacaa gatggcactg 3300
tacaacctct atcccggcgt gttcgagacc gtggagatgc tgccctccaa ggccggcatc 3360
tggagagtgg aatgcctgat cggcgagcac ctccacgctg ggatgtccac gctgttcctc 3420
gtttacagca ataagtgcca gacccctctg ggcatggcga gcggccacat ccgcgacttc 3480
cagattacag ccagcggcca gtacggtcag tgggctccaa agctggcccg tctgcactac 3540
tccggatcca tcaacgcctg gtccaccaag gaaccgttct cctggatcaa agtagacctg 3600
ctagccccca tgatcattca cggcatcaag acacaaggcg cccgacagaa gttctcgagc 3660
ctctatatct cccagttcat catcatgtat agcctggacg gaaagaagtg gcagacttac 3720
cgcggaaact cgacagggac cctgatggta ttcttcggta acgtggacag ctccggaatc 3780
aagcacaaca tcttcaaccc acccattatc gcccgctaca tccgcctgca ccccactcac 3840
tatagcatta ggtccaccct gcgaatggag ctcatgggct gtgacctgaa cagctgtagc 3900
atgcccctcg gcatggagtc taaggcgatc tccgacgcac agataacggc atcatcctac 3960
tttaccaaca tgttcgctac ctggtccccc tccaaggccc gactccacct gcaagggaga 4020
tccaacgcct ggcggccaca ggtcaacaat cccaaggagt ggctgcaagt ggactttcag 4080
aaaactatga aagtcaccgg agtgaccaca cagggagtga agtctctgct gaccagcatg 4140
tacgtgaagg agttcctcat ctccagttcg caggatggcc accagtggac gttgttcttc 4200
caaaacggta aagtcaaagt cttccaaggg aaccaggaca gctttacacc cgtcgtgaac 4260
tccctggacc ccccgcttct cactagatac ctccgcatcc accctcagag ctgggtgcac 4320
cagattgccc tgcgcatgga ggttctgggg tgtgaagccc aggacctgta ctaa 4374
<210> 8
<211> 4374
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 8
atgcagatcg aactgagcac ttgcttcttc ctgtgtctcc tgcgcttttg cttctccgcc 60
acaaggagat actatctcgg tgccgtggag ctcagctggg actacatgca gagcgacttg 120
ggtgaactgc ctgtggacgc caggtttcca ccccgcgtgc ccaagagttt cccgttcaac 180
accagtgtcg tgtacaagaa aaccctcttc gtggaattca ccgaccacct gttcaacatc 240
gccaaaccgc gccctccctg gatggggctg ctcggcccga cgatccaggc tgaggtctat 300
gacacggtgg tgattaccct caagaacatg gctagccacc cggtgagcct gcacgccgtg 360
ggcgtgtcct attggaaagc gtccgagggt gcggagtacg atgaccagac ttcacagcgg 420
gagaaggaag acgacaaagt gttccccggg ggttcccaca cctatgtctg gcaggtcctg 480
aaggagaatg gtcctatggc ctccgaccca ttgtgcctca cctactctta cctaagccat 540
gtggatctcg tcaaggacct gaactcgggg ctgatcggcg ccctgctcgt gtgccgggag 600
ggctcactgg ccaaggagaa gacccaaact ctgcacaagt tcatcctgct gttcgcggta 660
ttcgacgagg ggaagtcctg gcactccgag accaagaaca gcctgatgca ggaccgcgac 720
gcagcctcgg cccgtgcgtg gccaaagatg cacaccgtga acggctacgt taacaggagc 780
ctacccggcc tgatcggctg ccaccgcaaa tcggtctact ggcatgtgat cggaatgggc 840
acaacgcccg aggtccacag tatcttcctc gagggccaca ctttcctggt ccggaatcac 900
cgccaggcca gcctggagat cagccccata acctttctga cggcgcagac cttactcatg 960
gatctcggcc agttcctcct gttctgccac atttcgtccc accagcacga tgggatggaa 1020
gcatatgtga aagtggactc ctgccccgag gaaccccagc ttaggatgaa gaacaatgag 1080
gaggccgagg actacgacga tgaccttacc gattcagaaa tggacgtagt acgctttgac 1140
gacgacaact ctccatcctt catacagatt cgctccgtcg ccaagaagca ccctaagact 1200
tgggtgcact acatcgcggc cgaggaggag gactgggatt atgctcccct ggtgctggcc 1260
cccgacgacc gcagctacaa gagccagtac ctgaataacg ggccccagcg catcggccgg 1320
aagtacaaga aagtgcggtt catggcttac acggacgaga ccttcaagac ccgggaggct 1380
atccagcatg agagcggcat cttggggccc ctcctgtacg gcgaagttgg agacacactg 1440
ctgatcatct tcaagaacca ggcgagcagg ccctacaaca tctaccccca cggcattacc 1500
gatgtccggc cgttgtacag ccgacggctg cccaagggcg tgaagcacct gaaggacttt 1560
ccgatcctgc cgggcgagat cttcaagtac aagtggactg tgaccgtgga ggatgggccg 1620
accaagagcg atccgcgctg cctgacccgt tactactcca gctttgtcaa tatggagcgc 1680
gacctcgcta gcggcttgat tggccctctg ctgatctgct acaaggagtc cgtggaccag 1740
agggggaatc agatcatgag tgacaagagg aacgtgatcc tgttctccgt gttcgacgaa 1800
aaccgcagct ggtatctcac cgagaatatc cagcgcttcc tgcccaaccc ggccggtgtg 1860
cagctggagg accccgagtt tcaggccagc aacatcatgc attctatcaa cggatatgtg 1920
tttgattccc tgcagctctc agtgtgtctg cacgaggtcg cctactggta tatcctcagc 1980
attggggcac agaccgactt cctgagcgtg ttcttctccg ggtatacctt caagcacaag 2040
atggtgtacg aggataccct gaccctgttc ccctttagcg gcgaaaccgt gtttatgtct 2100
atggagaacc ccgggctctg gatccttgcg tgccataact ccgacttccg caaccgcgga 2160
atgaccgcgc tcctgaaagt gtcgagttgt gacaagaaca ccggcgacta ttacgaggac 2220
agttacgagg acatctctgc gtacctcctt agcaagaata acgccatcga gccaagatcc 2280
ttcagccaga accccccagt gctgaagagg catcagcggg agatcacccg cacgaccctg 2340
cagtcggatc aggaggagat tgattacgac gacacgatca gtgtggagat gaagaaggag 2400
gacttcgaca tctacgacga agatgaaaac cagtcccctc ggtccttcca aaagaagacc 2460
cggcactact tcatcgccgc tgtggaacgc ctgtgggact atggaatgtc ttctagccct 2520
cacgttttga ggaaccgcgc ccagtcgggc agcgtgcccc agttcaagaa agtggtgttc 2580
caggagttca ccgacggctc cttcacccag ccactttacc ggggcgagct caatgaacat 2640
ctgggcctgc tgggacccta catcagggct gaggtggagg acaacatcat ggtgacattc 2700
cggaatcagg ccagcagacc atacagtttc tacagttcac tcatctccta cgaggaggac 2760
cagcgccagg gggctgaacc ccgtaagaac ttcgtgaagc caaacgaaac aaagacctac 2820
ttctggaagg tccagcacca catggcacct accaaggacg agttcgattg caaggcctgg 2880
gcctacttct ccgacgtgga cctggagaaa gatgtgcaca gcggcctgat tggccctctg 2940
ctggtgtgtc acacgaacac actcaaccct gcacacgggc ggcaggtcac tgtgcaggaa 3000
ttcgccctgt tctttaccat ctttgatgag acgaagtcct ggtatttcac cgaaaacatg 3060
gagaggaact gccgcgcacc ctgcaacatc cagatggaag atccgacatt caaggagaac 3120
taccggttcc atgccatcaa tggctacatc atggacaccc tgcctggcct cgtgatggcc 3180
caagaccagc gtatccgctg gtatctgctg tcgatgggct ccaacgagaa catccatagt 3240
atccacttca gcgggcatgt cttcacggtg aggaaaaagg aggagtacaa gatggcactg 3300
tacaacctct atcccggcgt gttcgagacc gtggagatgc tgccctccaa ggccggcatc 3360
tggagagtgg aatgcctgat cggcgagcac ctccacgctg ggatgtccac gctgttcctc 3420
gtttacagca ataagtgcca gacccctctg ggcatggcga gcggccacat ccgcgacttc 3480
cagattacag ccagcggcca gtacggtcag tgggctccaa agctggcccg tctgcactac 3540
tccggatcca tcaacgcctg gtccaccaag gaaccgttct cctggatcaa agtagacctg 3600
ctagccccca tgatcattca cggcatcaag acacaaggcg cccgacagaa gttctcgagc 3660
ctctatatct cccagttcat catcatgtat agcctggacg gaaagaagtg gcagacttac 3720
cgcggaaact cgacagggac cctgatggta ttcttcggta acgtggacag ctccggaatc 3780
aagcacaaca tcttcaaccc acccattatc gcccgctaca tccgcctgca ccccactcac 3840
tatagcatta ggtccaccct gcgaatggag ctcatgggct gtgacctgaa cagctgtagc 3900
atgcccctcg gcatggagtc taaggcgatc tccgacgcac agataacggc atcatcctac 3960
tttaccaaca tgttcgctac ctggtccccc tccaaggccc gactccacct gcaagggaga 4020
tccaacgcct ggcggccaca ggtcaacaat cccaaggagt ggctgcaagt ggactttcag 4080
aaaactatga aagtcaccgg agtgaccaca cagggagtga agtctctgct gaccagcatg 4140
tacgtgaagg agttcctcat ctccagttcg caggatggcc accagtggac gttgttcttc 4200
caaaacggta aagtcaaagt cttccaaggg aaccaggaca gctttacacc cgtcgtgaac 4260
tccctggacc ccccgcttct cactagatac ctccgcatcc accctcagag ctgggtgcac 4320
cagattgccc tgcgcatgga ggttctgggg tgtgaagccc aggacctgta ctaa 4374
<210> 9
<211> 4395
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 9
atgcaaatag agctctccac ctgcttcttt ctgtgccttt tgcgattctg ctttagtgcc 60
accagaagat actacctggg tgcagtggaa ctgtcatggg actatatgca aagtgatctc 120
ggtgagctgc ctgtggacgc aagatttcct cctagagtgc caaaatcttt tccattcaac 180
acctcagtcg tgtacaaaaa gactctgttt gtagaattca cggatcacct tttcaacatc 240
gctaagccaa ggccaccctg gatgggtctg ctaggtccta ccatccaggc tgaggtttat 300
gatacagtgg tcattacact taagaacatg gcttcccatc ctgtcagtct tcatgctgtt 360
ggtgtatcct actggaaagc ttctgaggga gctgaatatg atgatcagac cagtcaaagg 420
gagaaagaag atgataaagt cttccctggt ggaagccata catatgtctg gcaggtcctg 480
aaagagaatg gtccaatggc ctctgaccca ctgtgcctta cctactcata tctttctcat 540
gtggacctgg taaaagactt gaattcaggc ctcattggag ccctactagt atgtagagaa 600
gggagtctgg ccaaggaaaa gacacagacc ttgcacaaat ttatactact ttttgctgta 660
tttgatgaag ggaaaagttg gcactcagaa acaaagaact ccttgatgca ggatagggat 720
gctgcatctg ctcgggcctg gcctaaaatg cacacagtca atggttatgt aaacaggtct 780
ctgccaggtc tgattggatg ccacaggaaa tcagtctatt ggcatgtgat tggaatgggc 840
accactcctg aagtgcactc aatattcctc gaaggtcaca catttcttgt gaggaaccat 900
cgccaggcgt ccttggaaat ctcgccaata actttcctta ctgctcaaac actcttgatg 960
gaccttggac agtttctact gttttgtcat atctcttccc accaacatga tggcatggaa 1020
gcttatgtca aagtagacag ctgtccagag gaaccccaac tacgaatgaa aaataatgaa 1080
gaagcggaag actatgatga tgatcttact gattctgaaa tggatgtggt caggtttgat 1140
gatgacaact ctccttcctt tatccaaatt cgctcagttg ccaagaagca tcctaaaact 1200
tgggtacatt acattgctgc tgaagaggag gactgggact atgctccctt agtcctcgcc 1260
cccgatgaca gaagttataa aagtcaatat ttgaacaatg gccctcagcg gattggtagg 1320
aagtacaaaa aagtccgatt tatggcatac acagatgaaa cctttaagac tcgtgaagct 1380
attcagcatg aatcaggaat cttgggacct ttactttatg gggaagttgg agacacactg 1440
ttgattatat ttaagaatca agcaagcaga ccatataaca tctaccctca cggaatcact 1500
gatgtccgtc ctttgtattc aaggagatta ccaaaaggtg taaaacattt gaaggatttt 1560
ccaattctgc caggagaaat attcaaatat aaatggacag tgactgtaga agatgggcca 1620
actaaatcag atcctcggtg cctgacccgc tattactcta gtttcgttaa tatggagaga 1680
gatctagctt caggactcat tggccctctc ctcatctgct acaaagaatc tgtagatcaa 1740
agaggaaacc agataatgtc agacaagagg aatgtcatcc tgttttctgt atttgatgag 1800
aaccgaagct ggtacctcac agagaatata caacgctttc tccccaatcc agctggagtg 1860
cagcttgagg atccagagtt ccaagcctcc aacatcatgc acagcatcaa tggctatgtt 1920
tttgatagtt tgcagttgtc agtttgtttg catgaggtgg catactggta cattctaagc 1980
attggagcac agactgactt cctttctgtc ttcttctctg gatatacctt caaacacaaa 2040
atggtctatg aagacacact caccctattc ccattctcag gagaaactgt cttcatgtcg 2100
atggaaaacc caggtctatg gattctggct tgccacaact cagactttcg gaacagaggc 2160
atgaccgcct tactgaaggt ttctagttgt gacaagaaca ctggtgatta ttacgaggac 2220
agttatgaag atatttcagc atacttgctg agtaaaaaca atgccattga accaagaagc 2280
ttctcccaga attcaagaca cccttctcaa aacccaccag tcttgaaacg ccatcaacgg 2340
gaaataactc gtactactct tcagtcagat caagaggaaa ttgactatga tgataccata 2400
tcagttgaaa tgaagaagga agattttgac atttatgatg aggatgaaaa tcagagcccc 2460
cgcagctttc aaaagaaaac acgacactat tttattgctg cagtggagag gctctgggat 2520
tatgggatga gtagctcccc acatgttcta agaaacaggg ctcagagtgg cagtgtccct 2580
cagttcaaga aagttgtttt ccaggaattt actgatggct cctttactca gcccttatac 2640
cgtggagaac taaatgaaca tttgggactc ctggggccat atataagagc agaagttgaa 2700
gataatatca tggtaacttt cagaaatcag gcctctcgtc cctattcctt ctattctagc 2760
cttatttctt atgaggaaga tcagaggcaa ggagcagaac ctagaaaaaa ctttgtcaag 2820
cctaatgaaa ccaaaactta cttttggaaa gtgcaacatc atatggcacc cactaaagat 2880
gagtttgact gcaaagcctg ggcttatttc tctgatgttg acctggaaaa agatgtgcac 2940
tcaggcctga ttggacccct tctggtctgc cacactaaca cactgaaccc tgctcatggg 3000
agacaagtga cagtacagga atttgctctg tttttcacca tctttgatga gaccaaaagc 3060
tggtacttca ctgaaaatat ggaaagaaac tgcagggctc cctgcaatat ccagatggaa 3120
gatcccactt ttaaagagaa ttatcgcttc catgcaatca atggctacat aatggataca 3180
ctacctggct tagtaatggc tcaggatcaa aggattcgat ggtatctgct cagcatgggc 3240
agcaatgaaa acatccattc tattcatttc agtggacatg tgttcactgt acgaaaaaaa 3300
gaggagtata aaatggcact gtacaatctc tatccaggtg tttttgagac agtggaaatg 3360
ttaccatcca aagctggaat ttggcgggtg gaatgcctta ttggcgagca tctacatgct 3420
gggatgagca cactttttct ggtgtacagc aataagtgtc agactcccct gggaatggct 3480
tctggacaca ttagagattt tcagattaca gcttcaggac aatatggaca gtgggcccca 3540
aagctggcca gacttcatta ttccggatca atcaatgcct ggagcaccaa ggagcccttt 3600
tcttggatca aggtggatct gttggcacca atgattattc acggcatcaa gacccagggt 3660
gcccgtcaga agttctccag cctctacatc tctcagttta tcatcatgta tagtcttgat 3720
gggaagaagt ggcagactta tcgaggaaat tccactggaa ccttaatggt cttctttggc 3780
aatgtggatt catctgggat aaaacacaat atttttaacc ctccaattat tgctcgatac 3840
atccgtttgc acccaactca ttatagcatt cgcagcactc ttcgcatgga gttgatgggc 3900
tgtgatttaa atagttgcag catgccattg ggaatggaga gtaaagcaat atcagatgca 3960
cagattactg cttcatccta ctttaccaat atgtttgcca cctggtctcc ttcaaaagct 4020
cgacttcacc tccaagggag gagtaatgcc tggagacctc aggtgaataa tccaaaagag 4080
tggctgcaag tggacttcca gaagacaatg aaagtcacag gagtaactac tcagggagta 4140
aaatctctgc ttaccagcat gtatgtgaag gagttcctca tctccagcag tcaagatggc 4200
catcagtgga ctctcttttt tcagaatggc aaagtaaagg tttttcaggg aaatcaagac 4260
tccttcacac ctgtggtgaa ctctctagac ccaccgttac tgactcgcta ccttcgaatt 4320
cacccccaga gttgggtgca ccagattgcc ctgaggatgg aggttctggg ctgcgaggca 4380
caggacctct actag 4395
<210> 10
<211> 4395
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 10
atgcaaatag agctctccac ctgcttcttt ctgtgccttt tgcgattctg ctttagtgcc 60
accagaagat actacctggg tgcagtggaa ctgtcatggg actatatgca aagtgatctc 120
ggtgagctgc ctgtggacgc aagatttcct cctagagtgc caaaatcttt tccattcaac 180
acctcagtcg tgtacaaaaa gactctgttt gtagaattca cggatcacct tttcaacatc 240
gctaagccaa ggccaccctg gatgggtctg ctaggtccta ccatccaggc tgaggtttat 300
gatacagtgg tcattacact taagaacatg gcttcccatc ctgtcagtct tcatgctgtt 360
ggtgtatcct actggaaagc ttctgaggga gctgaatatg atgatcagac cagtcaaagg 420
gagaaagaag atgataaagt cttccctggt ggaagccata catatgtctg gcaggtcctg 480
aaagagaatg gtccaatggc ctctgaccca ctgtgcctta cctactcata tctttctcat 540
gtggacctgg taaaagactt gaattcaggc ctcattggag ccctactagt atgtagagaa 600
gggagtctgg ccaaggaaaa gacacagacc ttgcacaaat ttatactact ttttgctgta 660
tttgatgaag ggaaaagttg gcactcagaa acaaagaact ccttgatgca ggatagggat 720
gctgcatctg ctcgggcctg gcctaaaatg cacacagtca atggttatgt aaacaggtct 780
ctgccaggtc tgattggatg ccacaggaaa tcagtctatt ggcatgtgat tggaatgggc 840
accactcctg aagtgcactc aatattcctc gaaggtcaca catttcttgt gaggaaccat 900
cgccaggcgt ccttggaaat ctcgccaata actttcctta ctgctcaaac actcttgatg 960
gaccttggac agtttctact gttttgtcat atctcttccc accaacatga tggcatggaa 1020
gcttatgtca aagtagacag ctgtccagag gaaccccaac tacgaatgaa aaataatgaa 1080
gaagcggaag actatgatga tgatcttact gattctgaaa tggatgtggt caggtttgat 1140
gatgacaact ctccttcctt tatccaaatt cgctcagttg ccaagaagca tcctaaaact 1200
tgggtacatt acattgctgc tgaagaggag gactgggact atgctccctt agtcctcgcc 1260
cccgatgaca gaagttataa aagtcaatat ttgaacaatg gccctcagcg gattggtagg 1320
aagtacaaaa aagtccgatt tatggcatac acagatgaaa cctttaagac tcgtgaagct 1380
attcagcatg aatcaggaat cttgggacct ttactttatg gggaagttgg agacacactg 1440
ttgattatat ttaagaatca agcaagcaga ccatataaca tctaccctca cggaatcact 1500
gatgtccgtc ctttgtattc aaggagatta ccaaaaggtg taaaacattt gaaggatttt 1560
ccaattctgc caggagaaat attcaaatat aaatggacag tgactgtaga agatgggcca 1620
actaaatcag atcctcggtg cctgacccgc tattactcta gtttcgttaa tatggagaga 1680
gatctagctt caggactcat tggccctctc ctcatctgct acaaagaatc tgtagatcaa 1740
agaggaaacc agataatgtc agacaagagg aatgtcatcc tgttttctgt atttgatgag 1800
aaccgaagct ggtacctcac agagaatata caacgctttc tccccaatcc agctggagtg 1860
cagcttgagg atccagagtt ccaagcctcc aacatcatgc acagcatcaa tggctatgtt 1920
tttgatagtt tgcagttgtc agtttgtttg catgaggtgg catactggta cattctaagc 1980
attggagcac agactgactt cctttctgtc ttcttctctg gatatacctt caaacacaaa 2040
atggtctatg aagacacact caccctattc ccattctcag gagaaactgt cttcatgtcg 2100
atggaaaacc caggtctatg gattctggcc tgccacaact cagactttcg gaacagaggc 2160
atgaccgcct tactgaaggt ttctagttgt gacaagaaca ctggtgatta ttacgaggac 2220
agttatgaag atatttcagc atacttgctg agtaaaaaca atgccattga accaagaagc 2280
ttctcccaga attcaagaca cccttctcaa aacccaccag tcttgaaacg ccatcaacgg 2340
gaaataactc gtactactct tcagtcagat caagaggaaa ttgactatga tgataccata 2400
tcagttgaaa tgaagaagga agattttgac atttatgatg aggatgaaaa tcagagcccc 2460
cgcagctttc aaaagaaaac acgacactat tttattgctg cagtggagag gctctgggat 2520
tatgggatga gtagctcccc acatgttcta agaaacaggg ctcagagtgg cagtgtccct 2580
cagttcaaga aagttgtttt ccaggaattt actgatggct cctttactca gcccttatac 2640
cgtggagaac taaatgaaca tttgggactc ctggggccat atataagagc agaagttgaa 2700
gataatatca tggtaacttt cagaaatcag gcctctcgtc cctattcctt ctattctagc 2760
cttatttctt atgaggaaga tcagaggcaa ggagcagaac ctagaaaaaa ctttgtcaag 2820
cctaatgaaa ccaaaactta cttttggaaa gtgcaacatc atatggcacc cactaaagat 2880
gagtttgact gcaaagcctg ggcttatttc tctgatgttg acctggaaaa agatgtgcac 2940
tcaggcctga ttggacccct tctggtctgc cacactaaca cactgaaccc tgctcatggg 3000
agacaagtga cagtacagga atttgctctg tttttcacca tctttgatga gaccaaaagc 3060
tggtacttca ctgaaaatat ggaaagaaac tgcagggctc cctgcaatat ccagatggaa 3120
gatcccactt ttaaagagaa ttatcgcttc catgcaatca atggctacat aatggataca 3180
ctacctggct tagtaatggc tcaggatcaa aggattcgat ggtatctgct cagcatgggc 3240
agcaatgaaa acatccattc tattcatttc agtggacatg tgttcactgt acgaaaaaaa 3300
gaggagtata aaatggcact gtacaatctc tatccaggtg tttttgagac agtggaaatg 3360
ttaccatcca aagctggaat ttggcgggtg gaatgcctta ttggcgagca tctacatgct 3420
gggatgagca cactttttct ggtgtacagc aataagtgtc agactcccct gggaatggct 3480
tctggacaca ttagagattt tcagattaca gcttcaggac aatatggaca gtgggcccca 3540
aagctggcca gacttcatta ttccggatca atcaatgcct ggagcaccaa ggagcccttt 3600
tcttggatca aggtggatct gttggcacca atgattattc acggcatcaa gacccagggt 3660
gcccgtcaga agttctccag cctctacatc tctcagttta tcatcatgta tagtcttgat 3720
gggaagaagt ggcagactta tcgaggaaat tccactggaa ccttaatggt cttctttggc 3780
aatgtggatt catctgggat aaaacacaat atttttaacc ctccaattat tgctcgatac 3840
atccgtttgc acccaactca ttatagcatt cgcagcactc ttcgcatgga gttgatgggc 3900
tgtgatttaa atagttgcag catgccattg ggaatggaga gtaaagcaat atcagatgca 3960
cagattactg cttcatccta ctttaccaat atgtttgcca cctggtctcc ttcaaaagct 4020
cgacttcacc tccaagggag gagtaatgcc tggagacctc aggtgaataa tccaaaagag 4080
tggctgcaag tggacttcca gaagacaatg aaagtcacag gagtaactac tcagggagta 4140
aaatctctgc ttaccagcat gtatgtgaag gagttcctca tctccagcag tcaagatggc 4200
catcagtgga ctctcttttt tcagaatggc aaagtaaagg tttttcaggg aaatcaagac 4260
tccttcacac ctgtggtgaa ctctctagac ccaccgttac tgactcgcta ccttcgaatt 4320
cacccccaga gttgggtgca ccagattgcc ctgaggatgg aggttctggg ctgcgaggca 4380
caggacctct actag 4395
<210> 11
<211> 4395
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 11
atgcaaatag agctctccac ctgcttcttt ctgtgccttt tgcgattctg ctttagtgcc 60
accagaagat actacctggg tgcagtggaa ctgtcatggg actatatgca aagtgatctc 120
ggtgagctgc ctgtggacgc aagatttcct cctagagtgc caaaatcttt tccattcaac 180
acctcagtcg tgtacaaaaa gactctgttt gtagaattca cggatcacct tttcaacatc 240
gctaagccaa ggccaccctg gatgggtctg ctaggtccta ccatccaggc tgaggtttat 300
gatacagtgg tcattacact taagaacatg gcttcccatc ctgtcagtct tcatgctgtt 360
ggtgtatcct actggaaagc ttctgaggga gctgaatatg atgatcagac cagtcaaagg 420
gagaaagaag atgataaagt cttccctggt ggaagccata catatgtctg gcaggtcctg 480
aaagagaatg gtccaatggc ctctgaccca ctgtgcctta cctactcata tctttctcat 540
gtggacctgg taaaagactt gaattcaggc ctcattggag ccctactagt atgtagagaa 600
gggagtctgg ccaaggaaaa gacacagacc ttgcacaaat ttatactact ttttgctgta 660
tttgatgaag ggaaaagttg gcactcagaa acaaagaact ccttgatgca ggatagggat 720
gctgcatctg ctcgggcctg gcctaaaatg cacacagtca atggttatgt aaacaggtct 780
ctgccaggtc tgattggatg ccacaggaaa tcagtctatt ggcatgtgat tggaatgggc 840
accactcctg aagtgcactc aatattcctc gaaggtcaca catttcttgt gaggaaccat 900
cgccaggcgt ccttggaaat ctcgccaata actttcctta ctgctcaaac actcttgatg 960
gaccttggac agtttctact gttttgtcat atctcttccc accaacatga tggcatggaa 1020
gcttatgtca aagtagacag ctgtccagag gaaccccaac tacgaatgaa aaataatgaa 1080
gaagcggaag actatgatga tgatcttact gattctgaaa tggatgtggt caggtttgat 1140
gatgacaact ctccttcctt tatccaaatt cgctcagttg ccaagaagca tcctaaaact 1200
tgggtacatt acattgctgc tgaagaggag gactgggact atgctccctt agtcctcgcc 1260
cccgatgaca gaagttataa aagtcaatat ttgaacaatg gccctcagcg gattggtagg 1320
aagtacaaaa aagtccgatt tatggcatac acagatgaaa cctttaagac tcgtgaagct 1380
attcagcatg aatcaggaat cttgggacct ttactttatg gggaagttgg agacacactg 1440
ttgattatat ttaagaatca agcaagcaga ccatataaca tctaccctca cggaatcact 1500
gatgtccgtc ctttgtattc aaggagatta ccaaaaggtg taaaacattt gaaggatttt 1560
ccaattctgc caggagaaat attcaaatat aaatggacag tgactgtaga agatgggcca 1620
actaaatcag atcctcggtg cctgacccgc tattactcta gtttcgttaa tatggagaga 1680
gatctagctt caggactcat tggccctctc ctcatctgct acaaagaatc tgtagatcaa 1740
agaggaaacc agataatgtc agacaagagg aatgtcatcc tgttttctgt atttgatgag 1800
aaccgaagct ggtacctcac agagaatata caacgctttc tccccaatcc agctggagtg 1860
cagcttgagg atccagagtt ccaagcctcc aacatcatgc acagcatcaa tggctatgtt 1920
tttgatagtt tgcagttgtc agtttgtttg catgaggtgg catactggta cattctaagc 1980
attggagcac agactgactt cctttctgtc ttcttctctg gatatacctt caaacacaaa 2040
atggtctatg aagacacact caccctattc ccattctcag gagaaactgt cttcatgtcg 2100
atggaaaacc caggtctatg gattctggca tgccacaact cagactttcg gaacagaggc 2160
atgaccgcct tactgaaggt ttctagttgt gacaagaaca ctggtgatta ttacgaggac 2220
agttatgaag atatttcagc atacttgctg agtaaaaaca atgccattga accaagaagc 2280
ttctcccaga attcaagaca cccttctcaa aacccaccag tcttgaaacg ccatcaacgg 2340
gaaataactc gtactactct tcagtcagat caagaggaaa ttgactatga tgataccata 2400
tcagttgaaa tgaagaagga agattttgac atttatgatg aggatgaaaa tcagagcccc 2460
cgcagctttc aaaagaaaac acgacactat tttattgctg cagtggagag gctctgggat 2520
tatgggatga gtagctcccc acatgttcta agaaacaggg ctcagagtgg cagtgtccct 2580
cagttcaaga aagttgtttt ccaggaattt actgatggct cctttactca gcccttatac 2640
cgtggagaac taaatgaaca tttgggactc ctggggccat atataagagc agaagttgaa 2700
gataatatca tggtaacttt cagaaatcag gcctctcgtc cctattcctt ctattctagc 2760
cttatttctt atgaggaaga tcagaggcaa ggagcagaac ctagaaaaaa ctttgtcaag 2820
cctaatgaaa ccaaaactta cttttggaaa gtgcaacatc atatggcacc cactaaagat 2880
gagtttgact gcaaagcctg ggcttatttc tctgatgttg acctggaaaa agatgtgcac 2940
tcaggcctga ttggacccct tctggtctgc cacactaaca cactgaaccc tgctcatggg 3000
agacaagtga cagtacagga atttgctctg tttttcacca tctttgatga gaccaaaagc 3060
tggtacttca ctgaaaatat ggaaagaaac tgcagggctc cctgcaatat ccagatggaa 3120
gatcccactt ttaaagagaa ttatcgcttc catgcaatca atggctacat aatggataca 3180
ctacctggct tagtaatggc tcaggatcaa aggattcgat ggtatctgct cagcatgggc 3240
agcaatgaaa acatccattc tattcatttc agtggacatg tgttcactgt acgaaaaaaa 3300
gaggagtata aaatggcact gtacaatctc tatccaggtg tttttgagac agtggaaatg 3360
ttaccatcca aagctggaat ttggcgggtg gaatgcctta ttggcgagca tctacatgct 3420
gggatgagca cactttttct ggtgtacagc aataagtgtc agactcccct gggaatggct 3480
tctggacaca ttagagattt tcagattaca gcttcaggac aatatggaca gtgggcccca 3540
aagctggcca gacttcatta ttccggatca atcaatgcct ggagcaccaa ggagcccttt 3600
tcttggatca aggtggatct gttggcacca atgattattc acggcatcaa gacccagggt 3660
gcccgtcaga agttctccag cctctacatc tctcagttta tcatcatgta tagtcttgat 3720
gggaagaagt ggcagactta tcgaggaaat tccactggaa ccttaatggt cttctttggc 3780
aatgtggatt catctgggat aaaacacaat atttttaacc ctccaattat tgctcgatac 3840
atccgtttgc acccaactca ttatagcatt cgcagcactc ttcgcatgga gttgatgggc 3900
tgtgatttaa atagttgcag catgccattg ggaatggaga gtaaagcaat atcagatgca 3960
cagattactg cttcatccta ctttaccaat atgtttgcca cctggtctcc ttcaaaagct 4020
cgacttcacc tccaagggag gagtaatgcc tggagacctc aggtgaataa tccaaaagag 4080
tggctgcaag tggacttcca gaagacaatg aaagtcacag gagtaactac tcagggagta 4140
aaatctctgc ttaccagcat gtatgtgaag gagttcctca tctccagcag tcaagatggc 4200
catcagtgga ctctcttttt tcagaatggc aaagtaaagg tttttcaggg aaatcaagac 4260
tccttcacac ctgtggtgaa ctctctagac ccaccgttac tgactcgcta ccttcgaatt 4320
cacccccaga gttgggtgca ccagattgcc ctgaggatgg aggttctggg ctgcgaggca 4380
caggacctct actag 4395
<210> 12
<211> 4395
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 12
atgcaaatag agctctccac ctgcttcttt ctgtgccttt tgcgattctg ctttagtgcc 60
accagaagat actacctggg tgcagtggaa ctgtcatggg actatatgca aagtgatctc 120
ggtgagctgc ctgtggacgc aagatttcct cctagagtgc caaaatcttt tccattcaac 180
acctcagtcg tgtacaaaaa gactctgttt gtagaattca cggatcacct tttcaacatc 240
gctaagccaa ggccaccctg gatgggtctg ctaggtccta ccatccaggc tgaggtttat 300
gatacagtgg tcattacact taagaacatg gcttcccatc ctgtcagtct tcatgctgtt 360
ggtgtatcct actggaaagc ttctgaggga gctgaatatg atgatcagac cagtcaaagg 420
gagaaagaag atgataaagt cttccctggt ggaagccata catatgtctg gcaggtcctg 480
aaagagaatg gtccaatggc ctctgaccca ctgtgcctta cctactcata tctttctcat 540
gtggacctgg taaaagactt gaattcaggc ctcattggag ccctactagt atgtagagaa 600
gggagtctgg ccaaggaaaa gacacagacc ttgcacaaat ttatactact ttttgctgta 660
tttgatgaag ggaaaagttg gcactcagaa acaaagaact ccttgatgca ggatagggat 720
gctgcatctg ctcgggcctg gcctaaaatg cacacagtca atggttatgt aaacaggtct 780
ctgccaggtc tgattggatg ccacaggaaa tcagtctatt ggcatgtgat tggaatgggc 840
accactcctg aagtgcactc aatattcctc gaaggtcaca catttcttgt gaggaaccat 900
cgccaggcgt ccttggaaat ctcgccaata actttcctta ctgctcaaac actcttgatg 960
gaccttggac agtttctact gttttgtcat atctcttccc accaacatga tggcatggaa 1020
gcttatgtca aagtagacag ctgtccagag gaaccccaac tacgaatgaa aaataatgaa 1080
gaagcggaag actatgatga tgatcttact gattctgaaa tggatgtggt caggtttgat 1140
gatgacaact ctccttcctt tatccaaatt cgctcagttg ccaagaagca tcctaaaact 1200
tgggtacatt acattgctgc tgaagaggag gactgggact atgctccctt agtcctcgcc 1260
cccgatgaca gaagttataa aagtcaatat ttgaacaatg gccctcagcg gattggtagg 1320
aagtacaaaa aagtccgatt tatggcatac acagatgaaa cctttaagac tcgtgaagct 1380
attcagcatg aatcaggaat cttgggacct ttactttatg gggaagttgg agacacactg 1440
ttgattatat ttaagaatca agcaagcaga ccatataaca tctaccctca cggaatcact 1500
gatgtccgtc ctttgtattc aaggagatta ccaaaaggtg taaaacattt gaaggatttt 1560
ccaattctgc caggagaaat attcaaatat aaatggacag tgactgtaga agatgggcca 1620
actaaatcag atcctcggtg cctgacccgc tattactcta gtttcgttaa tatggagaga 1680
gatctagctt caggactcat tggccctctc ctcatctgct acaaagaatc tgtagatcaa 1740
agaggaaacc agataatgtc agacaagagg aatgtcatcc tgttttctgt atttgatgag 1800
aaccgaagct ggtacctcac agagaatata caacgctttc tccccaatcc agctggagtg 1860
cagcttgagg atccagagtt ccaagcctcc aacatcatgc acagcatcaa tggctatgtt 1920
tttgatagtt tgcagttgtc agtttgtttg catgaggtgg catactggta cattctaagc 1980
attggagcac agactgactt cctttctgtc ttcttctctg gatatacctt caaacacaaa 2040
atggtctatg aagacacact caccctattc ccattctcag gagaaactgt cttcatgtcg 2100
atggaaaacc caggtctatg gattctggcg tgccacaact cagactttcg gaacagaggc 2160
atgaccgcct tactgaaggt ttctagttgt gacaagaaca ctggtgatta ttacgaggac 2220
agttatgaag atatttcagc atacttgctg agtaaaaaca atgccattga accaagaagc 2280
ttctcccaga attcaagaca cccttctcaa aacccaccag tcttgaaacg ccatcaacgg 2340
gaaataactc gtactactct tcagtcagat caagaggaaa ttgactatga tgataccata 2400
tcagttgaaa tgaagaagga agattttgac atttatgatg aggatgaaaa tcagagcccc 2460
cgcagctttc aaaagaaaac acgacactat tttattgctg cagtggagag gctctgggat 2520
tatgggatga gtagctcccc acatgttcta agaaacaggg ctcagagtgg cagtgtccct 2580
cagttcaaga aagttgtttt ccaggaattt actgatggct cctttactca gcccttatac 2640
cgtggagaac taaatgaaca tttgggactc ctggggccat atataagagc agaagttgaa 2700
gataatatca tggtaacttt cagaaatcag gcctctcgtc cctattcctt ctattctagc 2760
cttatttctt atgaggaaga tcagaggcaa ggagcagaac ctagaaaaaa ctttgtcaag 2820
cctaatgaaa ccaaaactta cttttggaaa gtgcaacatc atatggcacc cactaaagat 2880
gagtttgact gcaaagcctg ggcttatttc tctgatgttg acctggaaaa agatgtgcac 2940
tcaggcctga ttggacccct tctggtctgc cacactaaca cactgaaccc tgctcatggg 3000
agacaagtga cagtacagga atttgctctg tttttcacca tctttgatga gaccaaaagc 3060
tggtacttca ctgaaaatat ggaaagaaac tgcagggctc cctgcaatat ccagatggaa 3120
gatcccactt ttaaagagaa ttatcgcttc catgcaatca atggctacat aatggataca 3180
ctacctggct tagtaatggc tcaggatcaa aggattcgat ggtatctgct cagcatgggc 3240
agcaatgaaa acatccattc tattcatttc agtggacatg tgttcactgt acgaaaaaaa 3300
gaggagtata aaatggcact gtacaatctc tatccaggtg tttttgagac agtggaaatg 3360
ttaccatcca aagctggaat ttggcgggtg gaatgcctta ttggcgagca tctacatgct 3420
gggatgagca cactttttct ggtgtacagc aataagtgtc agactcccct gggaatggct 3480
tctggacaca ttagagattt tcagattaca gcttcaggac aatatggaca gtgggcccca 3540
aagctggcca gacttcatta ttccggatca atcaatgcct ggagcaccaa ggagcccttt 3600
tcttggatca aggtggatct gttggcacca atgattattc acggcatcaa gacccagggt 3660
gcccgtcaga agttctccag cctctacatc tctcagttta tcatcatgta tagtcttgat 3720
gggaagaagt ggcagactta tcgaggaaat tccactggaa ccttaatggt cttctttggc 3780
aatgtggatt catctgggat aaaacacaat atttttaacc ctccaattat tgctcgatac 3840
atccgtttgc acccaactca ttatagcatt cgcagcactc ttcgcatgga gttgatgggc 3900
tgtgatttaa atagttgcag catgccattg ggaatggaga gtaaagcaat atcagatgca 3960
cagattactg cttcatccta ctttaccaat atgtttgcca cctggtctcc ttcaaaagct 4020
cgacttcacc tccaagggag gagtaatgcc tggagacctc aggtgaataa tccaaaagag 4080
tggctgcaag tggacttcca gaagacaatg aaagtcacag gagtaactac tcagggagta 4140
aaatctctgc ttaccagcat gtatgtgaag gagttcctca tctccagcag tcaagatggc 4200
catcagtgga ctctcttttt tcagaatggc aaagtaaagg tttttcaggg aaatcaagac 4260
tccttcacac ctgtggtgaa ctctctagac ccaccgttac tgactcgcta ccttcgaatt 4320
cacccccaga gttgggtgca ccagattgcc ctgaggatgg aggttctggg ctgcgaggca 4380
caggacctct actag 4395
<210> 13
<211> 4392
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 13
atgcaaatag agctctccac ctgcttcttt ctgtgccttt tgcgattctg ctttagtgcc 60
accagaagat actacctggg tgcagtggaa ctgtcatggg actatatgca aagtgatctc 120
ggtgagctgc ctgtggacgc aagatttcct cctagagtgc caaaatcttt tccattcaac 180
acctcagtcg tgtacaaaaa gactctgttt gtagaattca cggatcacct tttcaacatc 240
gctaagccaa ggccaccctg gatgggtctg ctaggtccta ccatccaggc tgaggtttat 300
gatacagtgg tcattacact taagaacatg gcttcccatc ctgtcagtct tcatgctgtt 360
ggtgtatcct actggaaagc ttctgaggga gctgaatatg atgatcagac cagtcaaagg 420
gagaaagaag atgataaagt cttccctggt ggaagccata catatgtctg gcaggtcctg 480
aaagagaatg gtccaatggc ctctgaccca ctgtgcctta cctactcata tctttctcat 540
gtggacctgg taaaagactt gaattcaggc ctcattggag ccctactagt atgtagagaa 600
gggagtctgg ccaaggaaaa gacacagacc ttgcacaaat ttatactact ttttgctgta 660
tttgatgaag ggaaaagttg gcactcagaa acaaagaact ccttgatgca ggatagggat 720
gctgcatctg ctcgggcctg gcctaaaatg cacacagtca atggttatgt aaacaggtct 780
ctgccaggtc tgattggatg ccacaggaaa tcagtctatt ggcatgtgat tggaatgggc 840
accactcctg aagtgcactc aatattcctc gaaggtcaca catttcttgt gaggaaccat 900
cgccaggcgt ccttggaaat ctcgccaata actttcctta ctgctcaaac actcttgatg 960
gaccttggac agtttctact gttttgtcat atctcttccc accaacatga tggcatggaa 1020
gcttatgtca aagtagacag ctgtccagag gaaccccaac tacgaatgaa aaataatgaa 1080
gaagcggaag actatgatga tgatcttact gattctgaaa tggatgtggt caggtttgat 1140
gatgacaact ctccttcctt tatccaaatt cgctcagttg ccaagaagca tcctaaaact 1200
tgggtacatt acattgctgc tgaagaggag gactgggact atgctccctt agtcctcgcc 1260
cccgatgaca gaagttataa aagtcaatat ttgaacaatg gccctcagcg gattggtagg 1320
aagtacaaaa aagtccgatt tatggcatac acagatgaaa cctttaagac tcgtgaagct 1380
attcagcatg aatcaggaat cttgggacct ttactttatg gggaagttgg agacacactg 1440
ttgattatat ttaagaatca agcaagcaga ccatataaca tctaccctca cggaatcact 1500
gatgtccgtc ctttgtattc aaggagatta ccaaaaggtg taaaacattt gaaggatttt 1560
ccaattctgc caggagaaat attcaaatat aaatggacag tgactgtaga agatgggcca 1620
actaaatcag atcctcggtg cctgacccgc tattactcta gtttcgttaa tatggagaga 1680
gatctagctt caggactcat tggccctctc ctcatctgct acaaagaatc tgtagatcaa 1740
agaggaaacc agataatgtc agacaagagg aatgtcatcc tgttttctgt atttgatgag 1800
aaccgaagct ggtacctcac agagaatata caacgctttc tccccaatcc agctggagtg 1860
cagcttgagg atccagagtt ccaagcctcc aacatcatgc acagcatcaa tggctatgtt 1920
tttgatagtt tgcagttgtc agtttgtttg catgaggtgg catactggta cattctaagc 1980
attggagcac agactgactt cctttctgtc ttcttctctg gatatacctt caaacacaaa 2040
atggtctatg aagacacact caccctattc ccattctcag gagaaactgt cttcatgtcg 2100
atggaaaacc caggtctatg gattctggct tgccacaact cagactttcg gaacagaggc 2160
atgaccgcct tactgaaggt ttctagttgt gacaagaaca ctggtgatta ttacgaggac 2220
agttatgaag atatttcagc atacttgctg agtaaaaaca atgccattga accaagaagc 2280
ttctcccaga attcaagaca ccctagcact aggcaaaagc aatttaatgc caccacaatt 2340
ccagaaaata ctactcttca gtcagatcaa gaggaaattg actatgatga taccatatca 2400
gttgaaatga agaaggaaga ttttgacatt tatgatgagg atgaaaatca gagcccccgc 2460
agctttcaaa agaaaacacg acactatttt attgctgcag tggagaggct ctgggattat 2520
gggatgagta gctccccaca tgttctaaga aacagggctc agagtggcag tgtccctcag 2580
ttcaagaaag ttgttttcca ggaatttact gatggctcct ttactcagcc cttataccgt 2640
ggagaactaa atgaacattt gggactcctg gggccatata taagagcaga agttgaagat 2700
aatatcatgg taactttcag aaatcaggcc tctcgtccct attccttcta ttctagcctt 2760
atttcttatg aggaagatca gaggcaagga gcagaaccta gaaaaaactt tgtcaagcct 2820
aatgaaacca aaacttactt ttggaaagtg caacatcata tggcacccac taaagatgag 2880
tttgactgca aagcctgggc ttatttctct gatgttgacc tggaaaaaga tgtgcactca 2940
ggcctgattg gaccccttct ggtctgccac actaacacac tgaaccctgc tcatgggaga 3000
caagtgacag tacaggaatt tgctctgttt ttcaccatct ttgatgagac caaaagctgg 3060
tacttcactg aaaatatgga aagaaactgc agggctccct gcaatatcca gatggaagat 3120
cccactttta aagagaatta tcgcttccat gcaatcaatg gctacataat ggatacacta 3180
cctggcttag taatggctca ggatcaaagg attcgatggt atctgctcag catgggcagc 3240
aatgaaaaca tccattctat tcatttcagt ggacatgtgt tcactgtacg aaaaaaagag 3300
gagtataaaa tggcactgta caatctctat ccaggtgttt ttgagacagt ggaaatgtta 3360
ccatccaaag ctggaatttg gcgggtggaa tgccttattg gcgagcatct acatgctggg 3420
atgagcacac tttttctggt gtacagcaat aagtgtcaga ctcccctggg aatggcttct 3480
ggacacatta gagattttca gattacagct tcaggacaat atggacagtg ggccccaaag 3540
ctggccagac ttcattattc cggatcaatc aatgcctgga gcaccaagga gcccttttct 3600
tggatcaagg tggatctgtt ggcaccaatg attattcacg gcatcaagac ccagggtgcc 3660
cgtcagaagt tctccagcct ctacatctct cagtttatca tcatgtatag tcttgatggg 3720
aagaagtggc agacttatcg aggaaattcc actggaacct taatggtctt ctttggcaat 3780
gtggattcat ctgggataaa acacaatatt tttaaccctc caattattgc tcgatacatc 3840
cgtttgcacc caactcatta tagcattcgc agcactcttc gcatggagtt gatgggctgt 3900
gatttaaata gttgcagcat gccattggga atggagagta aagcaatatc agatgcacag 3960
attactgctt catcctactt taccaatatg tttgccacct ggtctccttc aaaagctcga 4020
cttcacctcc aagggaggag taatgcctgg agacctcagg tgaataatcc aaaagagtgg 4080
ctgcaagtgg acttccagaa gacaatgaaa gtcacaggag taactactca gggagtaaaa 4140
tctctgctta ccagcatgta tgtgaaggag ttcctcatct ccagcagtca agatggccat 4200
cagtggactc tcttttttca gaatggcaaa gtaaaggttt ttcagggaaa tcaagactcc 4260
ttcacacctg tggtgaactc tctagaccca ccgttactga ctcgctacct tcgaattcac 4320
ccccagagtt gggtgcacca gattgccctg aggatggagg ttctgggctg cgaggcacag 4380
gacctctact ag 4392
<210> 14
<211> 4392
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 14
atgcaaatag agctctccac ctgcttcttt ctgtgccttt tgcgattctg ctttagtgcc 60
accagaagat actacctggg tgcagtggaa ctgtcatggg actatatgca aagtgatctc 120
ggtgagctgc ctgtggacgc aagatttcct cctagagtgc caaaatcttt tccattcaac 180
acctcagtcg tgtacaaaaa gactctgttt gtagaattca cggatcacct tttcaacatc 240
gctaagccaa ggccaccctg gatgggtctg ctaggtccta ccatccaggc tgaggtttat 300
gatacagtgg tcattacact taagaacatg gcttcccatc ctgtcagtct tcatgctgtt 360
ggtgtatcct actggaaagc ttctgaggga gctgaatatg atgatcagac cagtcaaagg 420
gagaaagaag atgataaagt cttccctggt ggaagccata catatgtctg gcaggtcctg 480
aaagagaatg gtccaatggc ctctgaccca ctgtgcctta cctactcata tctttctcat 540
gtggacctgg taaaagactt gaattcaggc ctcattggag ccctactagt atgtagagaa 600
gggagtctgg ccaaggaaaa gacacagacc ttgcacaaat ttatactact ttttgctgta 660
tttgatgaag ggaaaagttg gcactcagaa acaaagaact ccttgatgca ggatagggat 720
gctgcatctg ctcgggcctg gcctaaaatg cacacagtca atggttatgt aaacaggtct 780
ctgccaggtc tgattggatg ccacaggaaa tcagtctatt ggcatgtgat tggaatgggc 840
accactcctg aagtgcactc aatattcctc gaaggtcaca catttcttgt gaggaaccat 900
cgccaggcgt ccttggaaat ctcgccaata actttcctta ctgctcaaac actcttgatg 960
gaccttggac agtttctact gttttgtcat atctcttccc accaacatga tggcatggaa 1020
gcttatgtca aagtagacag ctgtccagag gaaccccaac tacgaatgaa aaataatgaa 1080
gaagcggaag actatgatga tgatcttact gattctgaaa tggatgtggt caggtttgat 1140
gatgacaact ctccttcctt tatccaaatt cgctcagttg ccaagaagca tcctaaaact 1200
tgggtacatt acattgctgc tgaagaggag gactgggact atgctccctt agtcctcgcc 1260
cccgatgaca gaagttataa aagtcaatat ttgaacaatg gccctcagcg gattggtagg 1320
aagtacaaaa aagtccgatt tatggcatac acagatgaaa cctttaagac tcgtgaagct 1380
attcagcatg aatcaggaat cttgggacct ttactttatg gggaagttgg agacacactg 1440
ttgattatat ttaagaatca agcaagcaga ccatataaca tctaccctca cggaatcact 1500
gatgtccgtc ctttgtattc aaggagatta ccaaaaggtg taaaacattt gaaggatttt 1560
ccaattctgc caggagaaat attcaaatat aaatggacag tgactgtaga agatgggcca 1620
actaaatcag atcctcggtg cctgacccgc tattactcta gtttcgttaa tatggagaga 1680
gatctagctt caggactcat tggccctctc ctcatctgct acaaagaatc tgtagatcaa 1740
agaggaaacc agataatgtc agacaagagg aatgtcatcc tgttttctgt atttgatgag 1800
aaccgaagct ggtacctcac agagaatata caacgctttc tccccaatcc agctggagtg 1860
cagcttgagg atccagagtt ccaagcctcc aacatcatgc acagcatcaa tggctatgtt 1920
tttgatagtt tgcagttgtc agtttgtttg catgaggtgg catactggta cattctaagc 1980
attggagcac agactgactt cctttctgtc ttcttctctg gatatacctt caaacacaaa 2040
atggtctatg aagacacact caccctattc ccattctcag gagaaactgt cttcatgtcg 2100
atggaaaacc caggtctatg gattctggcc tgccacaact cagactttcg gaacagaggc 2160
atgaccgcct tactgaaggt ttctagttgt gacaagaaca ctggtgatta ttacgaggac 2220
agttatgaag atatttcagc atacttgctg agtaaaaaca atgccattga accaagaagc 2280
ttctcccaga attcaagaca ccctagcact aggcaaaagc aatttaatgc caccacaatt 2340
ccagaaaata ctactcttca gtcagatcaa gaggaaattg actatgatga taccatatca 2400
gttgaaatga agaaggaaga ttttgacatt tatgatgagg atgaaaatca gagcccccgc 2460
agctttcaaa agaaaacacg acactatttt attgctgcag tggagaggct ctgggattat 2520
gggatgagta gctccccaca tgttctaaga aacagggctc agagtggcag tgtccctcag 2580
ttcaagaaag ttgttttcca ggaatttact gatggctcct ttactcagcc cttataccgt 2640
ggagaactaa atgaacattt gggactcctg gggccatata taagagcaga agttgaagat 2700
aatatcatgg taactttcag aaatcaggcc tctcgtccct attccttcta ttctagcctt 2760
atttcttatg aggaagatca gaggcaagga gcagaaccta gaaaaaactt tgtcaagcct 2820
aatgaaacca aaacttactt ttggaaagtg caacatcata tggcacccac taaagatgag 2880
tttgactgca aagcctgggc ttatttctct gatgttgacc tggaaaaaga tgtgcactca 2940
ggcctgattg gaccccttct ggtctgccac actaacacac tgaaccctgc tcatgggaga 3000
caagtgacag tacaggaatt tgctctgttt ttcaccatct ttgatgagac caaaagctgg 3060
tacttcactg aaaatatgga aagaaactgc agggctccct gcaatatcca gatggaagat 3120
cccactttta aagagaatta tcgcttccat gcaatcaatg gctacataat ggatacacta 3180
cctggcttag taatggctca ggatcaaagg attcgatggt atctgctcag catgggcagc 3240
aatgaaaaca tccattctat tcatttcagt ggacatgtgt tcactgtacg aaaaaaagag 3300
gagtataaaa tggcactgta caatctctat ccaggtgttt ttgagacagt ggaaatgtta 3360
ccatccaaag ctggaatttg gcgggtggaa tgccttattg gcgagcatct acatgctggg 3420
atgagcacac tttttctggt gtacagcaat aagtgtcaga ctcccctggg aatggcttct 3480
ggacacatta gagattttca gattacagct tcaggacaat atggacagtg ggccccaaag 3540
ctggccagac ttcattattc cggatcaatc aatgcctgga gcaccaagga gcccttttct 3600
tggatcaagg tggatctgtt ggcaccaatg attattcacg gcatcaagac ccagggtgcc 3660
cgtcagaagt tctccagcct ctacatctct cagtttatca tcatgtatag tcttgatggg 3720
aagaagtggc agacttatcg aggaaattcc actggaacct taatggtctt ctttggcaat 3780
gtggattcat ctgggataaa acacaatatt tttaaccctc caattattgc tcgatacatc 3840
cgtttgcacc caactcatta tagcattcgc agcactcttc gcatggagtt gatgggctgt 3900
gatttaaata gttgcagcat gccattggga atggagagta aagcaatatc agatgcacag 3960
attactgctt catcctactt taccaatatg tttgccacct ggtctccttc aaaagctcga 4020
cttcacctcc aagggaggag taatgcctgg agacctcagg tgaataatcc aaaagagtgg 4080
ctgcaagtgg acttccagaa gacaatgaaa gtcacaggag taactactca gggagtaaaa 4140
tctctgctta ccagcatgta tgtgaaggag ttcctcatct ccagcagtca agatggccat 4200
cagtggactc tcttttttca gaatggcaaa gtaaaggttt ttcagggaaa tcaagactcc 4260
ttcacacctg tggtgaactc tctagaccca ccgttactga ctcgctacct tcgaattcac 4320
ccccagagtt gggtgcacca gattgccctg aggatggagg ttctgggctg cgaggcacag 4380
gacctctact ag 4392
<210> 15
<211> 4392
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 15
atgcaaatag agctctccac ctgcttcttt ctgtgccttt tgcgattctg ctttagtgcc 60
accagaagat actacctggg tgcagtggaa ctgtcatggg actatatgca aagtgatctc 120
ggtgagctgc ctgtggacgc aagatttcct cctagagtgc caaaatcttt tccattcaac 180
acctcagtcg tgtacaaaaa gactctgttt gtagaattca cggatcacct tttcaacatc 240
gctaagccaa ggccaccctg gatgggtctg ctaggtccta ccatccaggc tgaggtttat 300
gatacagtgg tcattacact taagaacatg gcttcccatc ctgtcagtct tcatgctgtt 360
ggtgtatcct actggaaagc ttctgaggga gctgaatatg atgatcagac cagtcaaagg 420
gagaaagaag atgataaagt cttccctggt ggaagccata catatgtctg gcaggtcctg 480
aaagagaatg gtccaatggc ctctgaccca ctgtgcctta cctactcata tctttctcat 540
gtggacctgg taaaagactt gaattcaggc ctcattggag ccctactagt atgtagagaa 600
gggagtctgg ccaaggaaaa gacacagacc ttgcacaaat ttatactact ttttgctgta 660
tttgatgaag ggaaaagttg gcactcagaa acaaagaact ccttgatgca ggatagggat 720
gctgcatctg ctcgggcctg gcctaaaatg cacacagtca atggttatgt aaacaggtct 780
ctgccaggtc tgattggatg ccacaggaaa tcagtctatt ggcatgtgat tggaatgggc 840
accactcctg aagtgcactc aatattcctc gaaggtcaca catttcttgt gaggaaccat 900
cgccaggcgt ccttggaaat ctcgccaata actttcctta ctgctcaaac actcttgatg 960
gaccttggac agtttctact gttttgtcat atctcttccc accaacatga tggcatggaa 1020
gcttatgtca aagtagacag ctgtccagag gaaccccaac tacgaatgaa aaataatgaa 1080
gaagcggaag actatgatga tgatcttact gattctgaaa tggatgtggt caggtttgat 1140
gatgacaact ctccttcctt tatccaaatt cgctcagttg ccaagaagca tcctaaaact 1200
tgggtacatt acattgctgc tgaagaggag gactgggact atgctccctt agtcctcgcc 1260
cccgatgaca gaagttataa aagtcaatat ttgaacaatg gccctcagcg gattggtagg 1320
aagtacaaaa aagtccgatt tatggcatac acagatgaaa cctttaagac tcgtgaagct 1380
attcagcatg aatcaggaat cttgggacct ttactttatg gggaagttgg agacacactg 1440
ttgattatat ttaagaatca agcaagcaga ccatataaca tctaccctca cggaatcact 1500
gatgtccgtc ctttgtattc aaggagatta ccaaaaggtg taaaacattt gaaggatttt 1560
ccaattctgc caggagaaat attcaaatat aaatggacag tgactgtaga agatgggcca 1620
actaaatcag atcctcggtg cctgacccgc tattactcta gtttcgttaa tatggagaga 1680
gatctagctt caggactcat tggccctctc ctcatctgct acaaagaatc tgtagatcaa 1740
agaggaaacc agataatgtc agacaagagg aatgtcatcc tgttttctgt atttgatgag 1800
aaccgaagct ggtacctcac agagaatata caacgctttc tccccaatcc agctggagtg 1860
cagcttgagg atccagagtt ccaagcctcc aacatcatgc acagcatcaa tggctatgtt 1920
tttgatagtt tgcagttgtc agtttgtttg catgaggtgg catactggta cattctaagc 1980
attggagcac agactgactt cctttctgtc ttcttctctg gatatacctt caaacacaaa 2040
atggtctatg aagacacact caccctattc ccattctcag gagaaactgt cttcatgtcg 2100
atggaaaacc caggtctatg gattctggca tgccacaact cagactttcg gaacagaggc 2160
atgaccgcct tactgaaggt ttctagttgt gacaagaaca ctggtgatta ttacgaggac 2220
agttatgaag atatttcagc atacttgctg agtaaaaaca atgccattga accaagaagc 2280
ttctcccaga attcaagaca ccctagcact aggcaaaagc aatttaatgc caccacaatt 2340
ccagaaaata ctactcttca gtcagatcaa gaggaaattg actatgatga taccatatca 2400
gttgaaatga agaaggaaga ttttgacatt tatgatgagg atgaaaatca gagcccccgc 2460
agctttcaaa agaaaacacg acactatttt attgctgcag tggagaggct ctgggattat 2520
gggatgagta gctccccaca tgttctaaga aacagggctc agagtggcag tgtccctcag 2580
ttcaagaaag ttgttttcca ggaatttact gatggctcct ttactcagcc cttataccgt 2640
ggagaactaa atgaacattt gggactcctg gggccatata taagagcaga agttgaagat 2700
aatatcatgg taactttcag aaatcaggcc tctcgtccct attccttcta ttctagcctt 2760
atttcttatg aggaagatca gaggcaagga gcagaaccta gaaaaaactt tgtcaagcct 2820
aatgaaacca aaacttactt ttggaaagtg caacatcata tggcacccac taaagatgag 2880
tttgactgca aagcctgggc ttatttctct gatgttgacc tggaaaaaga tgtgcactca 2940
ggcctgattg gaccccttct ggtctgccac actaacacac tgaaccctgc tcatgggaga 3000
caagtgacag tacaggaatt tgctctgttt ttcaccatct ttgatgagac caaaagctgg 3060
tacttcactg aaaatatgga aagaaactgc agggctccct gcaatatcca gatggaagat 3120
cccactttta aagagaatta tcgcttccat gcaatcaatg gctacataat ggatacacta 3180
cctggcttag taatggctca ggatcaaagg attcgatggt atctgctcag catgggcagc 3240
aatgaaaaca tccattctat tcatttcagt ggacatgtgt tcactgtacg aaaaaaagag 3300
gagtataaaa tggcactgta caatctctat ccaggtgttt ttgagacagt ggaaatgtta 3360
ccatccaaag ctggaatttg gcgggtggaa tgccttattg gcgagcatct acatgctggg 3420
atgagcacac tttttctggt gtacagcaat aagtgtcaga ctcccctggg aatggcttct 3480
ggacacatta gagattttca gattacagct tcaggacaat atggacagtg ggccccaaag 3540
ctggccagac ttcattattc cggatcaatc aatgcctgga gcaccaagga gcccttttct 3600
tggatcaagg tggatctgtt ggcaccaatg attattcacg gcatcaagac ccagggtgcc 3660
cgtcagaagt tctccagcct ctacatctct cagtttatca tcatgtatag tcttgatggg 3720
aagaagtggc agacttatcg aggaaattcc actggaacct taatggtctt ctttggcaat 3780
gtggattcat ctgggataaa acacaatatt tttaaccctc caattattgc tcgatacatc 3840
cgtttgcacc caactcatta tagcattcgc agcactcttc gcatggagtt gatgggctgt 3900
gatttaaata gttgcagcat gccattggga atggagagta aagcaatatc agatgcacag 3960
attactgctt catcctactt taccaatatg tttgccacct ggtctccttc aaaagctcga 4020
cttcacctcc aagggaggag taatgcctgg agacctcagg tgaataatcc aaaagagtgg 4080
ctgcaagtgg acttccagaa gacaatgaaa gtcacaggag taactactca gggagtaaaa 4140
tctctgctta ccagcatgta tgtgaaggag ttcctcatct ccagcagtca agatggccat 4200
cagtggactc tcttttttca gaatggcaaa gtaaaggttt ttcagggaaa tcaagactcc 4260
ttcacacctg tggtgaactc tctagaccca ccgttactga ctcgctacct tcgaattcac 4320
ccccagagtt gggtgcacca gattgccctg aggatggagg ttctgggctg cgaggcacag 4380
gacctctact ag 4392
<210> 16
<211> 4392
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 16
atgcaaatag agctctccac ctgcttcttt ctgtgccttt tgcgattctg ctttagtgcc 60
accagaagat actacctggg tgcagtggaa ctgtcatggg actatatgca aagtgatctc 120
ggtgagctgc ctgtggacgc aagatttcct cctagagtgc caaaatcttt tccattcaac 180
acctcagtcg tgtacaaaaa gactctgttt gtagaattca cggatcacct tttcaacatc 240
gctaagccaa ggccaccctg gatgggtctg ctaggtccta ccatccaggc tgaggtttat 300
gatacagtgg tcattacact taagaacatg gcttcccatc ctgtcagtct tcatgctgtt 360
ggtgtatcct actggaaagc ttctgaggga gctgaatatg atgatcagac cagtcaaagg 420
gagaaagaag atgataaagt cttccctggt ggaagccata catatgtctg gcaggtcctg 480
aaagagaatg gtccaatggc ctctgaccca ctgtgcctta cctactcata tctttctcat 540
gtggacctgg taaaagactt gaattcaggc ctcattggag ccctactagt atgtagagaa 600
gggagtctgg ccaaggaaaa gacacagacc ttgcacaaat ttatactact ttttgctgta 660
tttgatgaag ggaaaagttg gcactcagaa acaaagaact ccttgatgca ggatagggat 720
gctgcatctg ctcgggcctg gcctaaaatg cacacagtca atggttatgt aaacaggtct 780
ctgccaggtc tgattggatg ccacaggaaa tcagtctatt ggcatgtgat tggaatgggc 840
accactcctg aagtgcactc aatattcctc gaaggtcaca catttcttgt gaggaaccat 900
cgccaggcgt ccttggaaat ctcgccaata actttcctta ctgctcaaac actcttgatg 960
gaccttggac agtttctact gttttgtcat atctcttccc accaacatga tggcatggaa 1020
gcttatgtca aagtagacag ctgtccagag gaaccccaac tacgaatgaa aaataatgaa 1080
gaagcggaag actatgatga tgatcttact gattctgaaa tggatgtggt caggtttgat 1140
gatgacaact ctccttcctt tatccaaatt cgctcagttg ccaagaagca tcctaaaact 1200
tgggtacatt acattgctgc tgaagaggag gactgggact atgctccctt agtcctcgcc 1260
cccgatgaca gaagttataa aagtcaatat ttgaacaatg gccctcagcg gattggtagg 1320
aagtacaaaa aagtccgatt tatggcatac acagatgaaa cctttaagac tcgtgaagct 1380
attcagcatg aatcaggaat cttgggacct ttactttatg gggaagttgg agacacactg 1440
ttgattatat ttaagaatca agcaagcaga ccatataaca tctaccctca cggaatcact 1500
gatgtccgtc ctttgtattc aaggagatta ccaaaaggtg taaaacattt gaaggatttt 1560
ccaattctgc caggagaaat attcaaatat aaatggacag tgactgtaga agatgggcca 1620
actaaatcag atcctcggtg cctgacccgc tattactcta gtttcgttaa tatggagaga 1680
gatctagctt caggactcat tggccctctc ctcatctgct acaaagaatc tgtagatcaa 1740
agaggaaacc agataatgtc agacaagagg aatgtcatcc tgttttctgt atttgatgag 1800
aaccgaagct ggtacctcac agagaatata caacgctttc tccccaatcc agctggagtg 1860
cagcttgagg atccagagtt ccaagcctcc aacatcatgc acagcatcaa tggctatgtt 1920
tttgatagtt tgcagttgtc agtttgtttg catgaggtgg catactggta cattctaagc 1980
attggagcac agactgactt cctttctgtc ttcttctctg gatatacctt caaacacaaa 2040
atggtctatg aagacacact caccctattc ccattctcag gagaaactgt cttcatgtcg 2100
atggaaaacc caggtctatg gattctggcg tgccacaact cagactttcg gaacagaggc 2160
atgaccgcct tactgaaggt ttctagttgt gacaagaaca ctggtgatta ttacgaggac 2220
agttatgaag atatttcagc atacttgctg agtaaaaaca atgccattga accaagaagc 2280
ttctcccaga attcaagaca ccctagcact aggcaaaagc aatttaatgc caccacaatt 2340
ccagaaaata ctactcttca gtcagatcaa gaggaaattg actatgatga taccatatca 2400
gttgaaatga agaaggaaga ttttgacatt tatgatgagg atgaaaatca gagcccccgc 2460
agctttcaaa agaaaacacg acactatttt attgctgcag tggagaggct ctgggattat 2520
gggatgagta gctccccaca tgttctaaga aacagggctc agagtggcag tgtccctcag 2580
ttcaagaaag ttgttttcca ggaatttact gatggctcct ttactcagcc cttataccgt 2640
ggagaactaa atgaacattt gggactcctg gggccatata taagagcaga agttgaagat 2700
aatatcatgg taactttcag aaatcaggcc tctcgtccct attccttcta ttctagcctt 2760
atttcttatg aggaagatca gaggcaagga gcagaaccta gaaaaaactt tgtcaagcct 2820
aatgaaacca aaacttactt ttggaaagtg caacatcata tggcacccac taaagatgag 2880
tttgactgca aagcctgggc ttatttctct gatgttgacc tggaaaaaga tgtgcactca 2940
ggcctgattg gaccccttct ggtctgccac actaacacac tgaaccctgc tcatgggaga 3000
caagtgacag tacaggaatt tgctctgttt ttcaccatct ttgatgagac caaaagctgg 3060
tacttcactg aaaatatgga aagaaactgc agggctccct gcaatatcca gatggaagat 3120
cccactttta aagagaatta tcgcttccat gcaatcaatg gctacataat ggatacacta 3180
cctggcttag taatggctca ggatcaaagg attcgatggt atctgctcag catgggcagc 3240
aatgaaaaca tccattctat tcatttcagt ggacatgtgt tcactgtacg aaaaaaagag 3300
gagtataaaa tggcactgta caatctctat ccaggtgttt ttgagacagt ggaaatgtta 3360
ccatccaaag ctggaatttg gcgggtggaa tgccttattg gcgagcatct acatgctggg 3420
atgagcacac tttttctggt gtacagcaat aagtgtcaga ctcccctggg aatggcttct 3480
ggacacatta gagattttca gattacagct tcaggacaat atggacagtg ggccccaaag 3540
ctggccagac ttcattattc cggatcaatc aatgcctgga gcaccaagga gcccttttct 3600
tggatcaagg tggatctgtt ggcaccaatg attattcacg gcatcaagac ccagggtgcc 3660
cgtcagaagt tctccagcct ctacatctct cagtttatca tcatgtatag tcttgatggg 3720
aagaagtggc agacttatcg aggaaattcc actggaacct taatggtctt ctttggcaat 3780
gtggattcat ctgggataaa acacaatatt tttaaccctc caattattgc tcgatacatc 3840
cgtttgcacc caactcatta tagcattcgc agcactcttc gcatggagtt gatgggctgt 3900
gatttaaata gttgcagcat gccattggga atggagagta aagcaatatc agatgcacag 3960
attactgctt catcctactt taccaatatg tttgccacct ggtctccttc aaaagctcga 4020
cttcacctcc aagggaggag taatgcctgg agacctcagg tgaataatcc aaaagagtgg 4080
ctgcaagtgg acttccagaa gacaatgaaa gtcacaggag taactactca gggagtaaaa 4140
tctctgctta ccagcatgta tgtgaaggag ttcctcatct ccagcagtca agatggccat 4200
cagtggactc tcttttttca gaatggcaaa gtaaaggttt ttcagggaaa tcaagactcc 4260
ttcacacctg tggtgaactc tctagaccca ccgttactga ctcgctacct tcgaattcac 4320
ccccagagtt gggtgcacca gattgccctg aggatggagg ttctgggctg cgaggcacag 4380
gacctctact ag 4392
<210> 17
<211> 4362
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 17
atgcagatcg aactgagcac ttgcttcttc ctgtgtctcc tgcgcttttg cttctccgcc 60
acaaggagat actatctcgg tgccgtggag ctcagctggg actacatgca gagcgacttg 120
ggtgaactgc ctgtggacgc caggtttcca ccccgcgtgc ccaagagttt cccgttcaac 180
accagtgtcg tgtacaagaa aaccctcttc gtggaattca ccgaccacct gttcaacatc 240
gccaaaccgc gccctccctg gatggggctg ctcggcccga cgatccaggc tgaggtctat 300
gacacggtgg tgattaccct caagaacatg gctagccacc cggtgagcct gcacgccgtg 360
ggcgtgtcct attggaaagc gtccgagggt gcggagtacg atgaccagac ttcacagcgg 420
gagaaggaag acgacaaagt gttccccggg ggttcccaca cctatgtctg gcaggtcctg 480
aaggagaatg gtcctatggc ctccgaccca ttgtgcctca cctactctta cctaagccat 540
gtggatctcg tcaaggacct gaactcgggg ctgatcggcg ccctgctcgt gtgccgggag 600
ggctcactgg ccaaggagaa gacccaaact ctgcacaagt tcatcctgct gttcgcggta 660
ttcgacgagg ggaagtcctg gcactccgag accaagaaca gcctgatgca ggaccgcgac 720
gcagcctcgg cccgtgcgtg gccaaagatg cacaccgtga acggctacgt taacaggagc 780
ctacccggcc tgatcggctg ccaccgcaaa tcggtctact ggcatgtgat cggaatgggc 840
acaacgcccg aggtccacag tatcttcctc gagggccaca ctttcctggt ccggaatcac 900
cgccaggcca gcctggagat cagccccata acctttctga cggcgcagac cttactcatg 960
gatctcggcc agttcctcct gttctgccac atttcgtccc accagcacga tgggatggaa 1020
gcatatgtga aagtggactc ctgccccgag gaaccccagc ttaggatgaa gaacaatgag 1080
gaggccgagg actacgacga tgaccttacc gattcagaaa tggacgtagt acgctttgac 1140
gacgacaact ctccatcctt catacagatt cgctccgtcg ccaagaagca ccctaagact 1200
tgggtgcact acatcgcggc cgaggaggag gactgggatt atgctcccct ggtgctggcc 1260
cccgacgacc gcagctacaa gagccagtac ctgaataacg ggccccagcg catcggccgg 1320
aagtacaaga aagtgcggtt catggcttac acggacgaga ccttcaagac ccgggaggct 1380
atccagcatg agagcggcat cttggggccc ctcctgtacg gcgaagttgg agacacactg 1440
ctgatcatct tcaagaacca ggcgagcagg ccctacaaca tctaccccca cggcattacc 1500
gatgtccggc cgttgtacag ccgacggctg cccaagggcg tgaagcacct gaaggacttt 1560
ccgatcctgc cgggcgagat cttcaagtac aagtggactg tgaccgtgga ggatgggccg 1620
accaagagcg atccgcgctg cctgacccgt tactactcca gctttgtcaa tatggagcgc 1680
gacctcgcta gcggcttgat tggccctctg ctgatctgct acaaggagtc cgtggaccag 1740
agggggaatc agatcatgag tgacaagagg aacgtgatcc tgttctccgt gttcgacgaa 1800
aaccgcagct ggtatctcac cgagaatatc cagcgcttcc tgcccaaccc ggccggtgtg 1860
cagctggagg accccgagtt tcaggccagc aacatcatgc attctatcaa cggatatgtg 1920
tttgattccc tgcagctctc agtgtgtctg cacgaggtcg cctactggta tatcctcagc 1980
attggggcac agaccgactt cctgagcgtg ttcttctccg ggtatacctt caagcacaag 2040
atggtgtacg aggataccct gaccctgttc ccctttagcg gcgaaaccgt gtttatgtct 2100
atggagaacc ccgggctctg gatccttgct tgccataact ccgacttccg caaccgcgga 2160
atgaccgcgc tcctgaaagt gtcgagttgt gacaagaaca ccggcgacta ttacgaggac 2220
agttacgagg acatctctgc gtacctcctt agcaagaata acgccatcga gccaagatcc 2280
ttcagccaga accccccagt gctgaaggag atcacccgca cgaccctgca gtcggatcag 2340
gaggagattg attacgacga cacgatcagt gtggagatga agaaggagga cttcgacatc 2400
tacgacgaag atgaaaacca gtcccctcgg tccttccaaa agaagacccg gcactacttc 2460
atcgccgctg tggaacgcct gtgggactat ggaatgtctt ctagccctca cgttttgagg 2520
aaccgcgccc agtcgggcag cgtgccccag ttcaagaaag tggtgttcca ggagttcacc 2580
gacggctcct tcacccagcc actttaccgg ggcgagctca atgaacatct gggcctgctg 2640
ggaccctaca tcagggctga ggtggaggac aacatcatgg tgacattccg gaatcaggcc 2700
agcagaccat acagtttcta cagttcactc atctcctacg aggaggacca gcgccagggg 2760
gctgaacccc gtaagaactt cgtgaagcca aacgaaacaa agacctactt ctggaaggtc 2820
cagcaccaca tggcacctac caaggacgag ttcgattgca aggcctgggc ctacttctcc 2880
gacgtggacc tggagaaaga tgtgcacagc ggcctgattg gccctctgct ggtgtgtcac 2940
acgaacacac tcaaccctgc acacgggcgg caggtcactg tgcaggaatt cgccctgttc 3000
tttaccatct ttgatgagac gaagtcctgg tatttcaccg aaaacatgga gaggaactgc 3060
cgcgcaccct gcaacatcca gatggaagat ccgacattca aggagaacta ccggttccat 3120
gccatcaatg gctacatcat ggacaccctg cctggcctcg tgatggccca agaccagcgt 3180
atccgctggt atctgctgtc gatgggctcc aacgagaaca tccatagtat ccacttcagc 3240
gggcatgtct tcacggtgag gaaaaaggag gagtacaaga tggcactgta caacctctat 3300
cccggcgtgt tcgagaccgt ggagatgctg ccctccaagg ccggcatctg gagagtggaa 3360
tgcctgatcg gcgagcacct ccacgctggg atgtccacgc tgttcctcgt ttacagcaat 3420
aagtgccaga cccctctggg catggcgagc ggccacatcc gcgacttcca gattacagcc 3480
agcggccagt acggtcagtg ggctccaaag ctggcccgtc tgcactactc cggatccatc 3540
aacgcctggt ccaccaagga accgttctcc tggatcaaag tagacctgct agcccccatg 3600
atcattcacg gcatcaagac acaaggcgcc cgacagaagt tctcgagcct ctatatctcc 3660
cagttcatca tcatgtatag cctggacgga aagaagtggc agacttaccg cggaaactcg 3720
acagggaccc tgatggtatt cttcggtaac gtggacagct ccggaatcaa gcacaacatc 3780
ttcaacccac ccattatcgc ccgctacatc cgcctgcacc ccactcacta tagcattagg 3840
tccaccctgc gaatggagct catgggctgt gacctgaaca gctgtagcat gcccctcggc 3900
atggagtcta aggcgatctc cgacgcacag ataacggcat catcctactt taccaacatg 3960
ttcgctacct ggtccccctc caaggcccga ctccacctgc aagggagatc caacgcctgg 4020
cggccacagg tcaacaatcc caaggagtgg ctgcaagtgg actttcagaa aactatgaaa 4080
gtcaccggag tgaccacaca gggagtgaag tctctgctga ccagcatgta cgtgaaggag 4140
ttcctcatct ccagttcgca ggatggccac cagtggacgt tgttcttcca aaacggtaaa 4200
gtcaaagtct tccaagggaa ccaggacagc tttacacccg tcgtgaactc cctggacccc 4260
ccgcttctca ctagatacct ccgcatccac cctcagagct gggtgcacca gattgccctg 4320
cgcatggagg ttctggggtg tgaagcccag gacctgtact aa 4362
<210> 18
<211> 4362
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 18
atgcagatcg aactgagcac ttgcttcttc ctgtgtctcc tgcgcttttg cttctccgcc 60
acaaggagat actatctcgg tgccgtggag ctcagctggg actacatgca gagcgacttg 120
ggtgaactgc ctgtggacgc caggtttcca ccccgcgtgc ccaagagttt cccgttcaac 180
accagtgtcg tgtacaagaa aaccctcttc gtggaattca ccgaccacct gttcaacatc 240
gccaaaccgc gccctccctg gatggggctg ctcggcccga cgatccaggc tgaggtctat 300
gacacggtgg tgattaccct caagaacatg gctagccacc cggtgagcct gcacgccgtg 360
ggcgtgtcct attggaaagc gtccgagggt gcggagtacg atgaccagac ttcacagcgg 420
gagaaggaag acgacaaagt gttccccggg ggttcccaca cctatgtctg gcaggtcctg 480
aaggagaatg gtcctatggc ctccgaccca ttgtgcctca cctactctta cctaagccat 540
gtggatctcg tcaaggacct gaactcgggg ctgatcggcg ccctgctcgt gtgccgggag 600
ggctcactgg ccaaggagaa gacccaaact ctgcacaagt tcatcctgct gttcgcggta 660
ttcgacgagg ggaagtcctg gcactccgag accaagaaca gcctgatgca ggaccgcgac 720
gcagcctcgg cccgtgcgtg gccaaagatg cacaccgtga acggctacgt taacaggagc 780
ctacccggcc tgatcggctg ccaccgcaaa tcggtctact ggcatgtgat cggaatgggc 840
acaacgcccg aggtccacag tatcttcctc gagggccaca ctttcctggt ccggaatcac 900
cgccaggcca gcctggagat cagccccata acctttctga cggcgcagac cttactcatg 960
gatctcggcc agttcctcct gttctgccac atttcgtccc accagcacga tgggatggaa 1020
gcatatgtga aagtggactc ctgccccgag gaaccccagc ttaggatgaa gaacaatgag 1080
gaggccgagg actacgacga tgaccttacc gattcagaaa tggacgtagt acgctttgac 1140
gacgacaact ctccatcctt catacagatt cgctccgtcg ccaagaagca ccctaagact 1200
tgggtgcact acatcgcggc cgaggaggag gactgggatt atgctcccct ggtgctggcc 1260
cccgacgacc gcagctacaa gagccagtac ctgaataacg ggccccagcg catcggccgg 1320
aagtacaaga aagtgcggtt catggcttac acggacgaga ccttcaagac ccgggaggct 1380
atccagcatg agagcggcat cttggggccc ctcctgtacg gcgaagttgg agacacactg 1440
ctgatcatct tcaagaacca ggcgagcagg ccctacaaca tctaccccca cggcattacc 1500
gatgtccggc cgttgtacag ccgacggctg cccaagggcg tgaagcacct gaaggacttt 1560
ccgatcctgc cgggcgagat cttcaagtac aagtggactg tgaccgtgga ggatgggccg 1620
accaagagcg atccgcgctg cctgacccgt tactactcca gctttgtcaa tatggagcgc 1680
gacctcgcta gcggcttgat tggccctctg ctgatctgct acaaggagtc cgtggaccag 1740
agggggaatc agatcatgag tgacaagagg aacgtgatcc tgttctccgt gttcgacgaa 1800
aaccgcagct ggtatctcac cgagaatatc cagcgcttcc tgcccaaccc ggccggtgtg 1860
cagctggagg accccgagtt tcaggccagc aacatcatgc attctatcaa cggatatgtg 1920
tttgattccc tgcagctctc agtgtgtctg cacgaggtcg cctactggta tatcctcagc 1980
attggggcac agaccgactt cctgagcgtg ttcttctccg ggtatacctt caagcacaag 2040
atggtgtacg aggataccct gaccctgttc ccctttagcg gcgaaaccgt gtttatgtct 2100
atggagaacc ccgggctctg gatccttgcc tgccataact ccgacttccg caaccgcgga 2160
atgaccgcgc tcctgaaagt gtcgagttgt gacaagaaca ccggcgacta ttacgaggac 2220
agttacgagg acatctctgc gtacctcctt agcaagaata acgccatcga gccaagatcc 2280
ttcagccaga accccccagt gctgaaggag atcacccgca cgaccctgca gtcggatcag 2340
gaggagattg attacgacga cacgatcagt gtggagatga agaaggagga cttcgacatc 2400
tacgacgaag atgaaaacca gtcccctcgg tccttccaaa agaagacccg gcactacttc 2460
atcgccgctg tggaacgcct gtgggactat ggaatgtctt ctagccctca cgttttgagg 2520
aaccgcgccc agtcgggcag cgtgccccag ttcaagaaag tggtgttcca ggagttcacc 2580
gacggctcct tcacccagcc actttaccgg ggcgagctca atgaacatct gggcctgctg 2640
ggaccctaca tcagggctga ggtggaggac aacatcatgg tgacattccg gaatcaggcc 2700
agcagaccat acagtttcta cagttcactc atctcctacg aggaggacca gcgccagggg 2760
gctgaacccc gtaagaactt cgtgaagcca aacgaaacaa agacctactt ctggaaggtc 2820
cagcaccaca tggcacctac caaggacgag ttcgattgca aggcctgggc ctacttctcc 2880
gacgtggacc tggagaaaga tgtgcacagc ggcctgattg gccctctgct ggtgtgtcac 2940
acgaacacac tcaaccctgc acacgggcgg caggtcactg tgcaggaatt cgccctgttc 3000
tttaccatct ttgatgagac gaagtcctgg tatttcaccg aaaacatgga gaggaactgc 3060
cgcgcaccct gcaacatcca gatggaagat ccgacattca aggagaacta ccggttccat 3120
gccatcaatg gctacatcat ggacaccctg cctggcctcg tgatggccca agaccagcgt 3180
atccgctggt atctgctgtc gatgggctcc aacgagaaca tccatagtat ccacttcagc 3240
gggcatgtct tcacggtgag gaaaaaggag gagtacaaga tggcactgta caacctctat 3300
cccggcgtgt tcgagaccgt ggagatgctg ccctccaagg ccggcatctg gagagtggaa 3360
tgcctgatcg gcgagcacct ccacgctggg atgtccacgc tgttcctcgt ttacagcaat 3420
aagtgccaga cccctctggg catggcgagc ggccacatcc gcgacttcca gattacagcc 3480
agcggccagt acggtcagtg ggctccaaag ctggcccgtc tgcactactc cggatccatc 3540
aacgcctggt ccaccaagga accgttctcc tggatcaaag tagacctgct agcccccatg 3600
atcattcacg gcatcaagac acaaggcgcc cgacagaagt tctcgagcct ctatatctcc 3660
cagttcatca tcatgtatag cctggacgga aagaagtggc agacttaccg cggaaactcg 3720
acagggaccc tgatggtatt cttcggtaac gtggacagct ccggaatcaa gcacaacatc 3780
ttcaacccac ccattatcgc ccgctacatc cgcctgcacc ccactcacta tagcattagg 3840
tccaccctgc gaatggagct catgggctgt gacctgaaca gctgtagcat gcccctcggc 3900
atggagtcta aggcgatctc cgacgcacag ataacggcat catcctactt taccaacatg 3960
ttcgctacct ggtccccctc caaggcccga ctccacctgc aagggagatc caacgcctgg 4020
cggccacagg tcaacaatcc caaggagtgg ctgcaagtgg actttcagaa aactatgaaa 4080
gtcaccggag tgaccacaca gggagtgaag tctctgctga ccagcatgta cgtgaaggag 4140
ttcctcatct ccagttcgca ggatggccac cagtggacgt tgttcttcca aaacggtaaa 4200
gtcaaagtct tccaagggaa ccaggacagc tttacacccg tcgtgaactc cctggacccc 4260
ccgcttctca ctagatacct ccgcatccac cctcagagct gggtgcacca gattgccctg 4320
cgcatggagg ttctggggtg tgaagcccag gacctgtact aa 4362
<210> 19
<211> 4362
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 19
atgcagatcg aactgagcac ttgcttcttc ctgtgtctcc tgcgcttttg cttctccgcc 60
acaaggagat actatctcgg tgccgtggag ctcagctggg actacatgca gagcgacttg 120
ggtgaactgc ctgtggacgc caggtttcca ccccgcgtgc ccaagagttt cccgttcaac 180
accagtgtcg tgtacaagaa aaccctcttc gtggaattca ccgaccacct gttcaacatc 240
gccaaaccgc gccctccctg gatggggctg ctcggcccga cgatccaggc tgaggtctat 300
gacacggtgg tgattaccct caagaacatg gctagccacc cggtgagcct gcacgccgtg 360
ggcgtgtcct attggaaagc gtccgagggt gcggagtacg atgaccagac ttcacagcgg 420
gagaaggaag acgacaaagt gttccccggg ggttcccaca cctatgtctg gcaggtcctg 480
aaggagaatg gtcctatggc ctccgaccca ttgtgcctca cctactctta cctaagccat 540
gtggatctcg tcaaggacct gaactcgggg ctgatcggcg ccctgctcgt gtgccgggag 600
ggctcactgg ccaaggagaa gacccaaact ctgcacaagt tcatcctgct gttcgcggta 660
ttcgacgagg ggaagtcctg gcactccgag accaagaaca gcctgatgca ggaccgcgac 720
gcagcctcgg cccgtgcgtg gccaaagatg cacaccgtga acggctacgt taacaggagc 780
ctacccggcc tgatcggctg ccaccgcaaa tcggtctact ggcatgtgat cggaatgggc 840
acaacgcccg aggtccacag tatcttcctc gagggccaca ctttcctggt ccggaatcac 900
cgccaggcca gcctggagat cagccccata acctttctga cggcgcagac cttactcatg 960
gatctcggcc agttcctcct gttctgccac atttcgtccc accagcacga tgggatggaa 1020
gcatatgtga aagtggactc ctgccccgag gaaccccagc ttaggatgaa gaacaatgag 1080
gaggccgagg actacgacga tgaccttacc gattcagaaa tggacgtagt acgctttgac 1140
gacgacaact ctccatcctt catacagatt cgctccgtcg ccaagaagca ccctaagact 1200
tgggtgcact acatcgcggc cgaggaggag gactgggatt atgctcccct ggtgctggcc 1260
cccgacgacc gcagctacaa gagccagtac ctgaataacg ggccccagcg catcggccgg 1320
aagtacaaga aagtgcggtt catggcttac acggacgaga ccttcaagac ccgggaggct 1380
atccagcatg agagcggcat cttggggccc ctcctgtacg gcgaagttgg agacacactg 1440
ctgatcatct tcaagaacca ggcgagcagg ccctacaaca tctaccccca cggcattacc 1500
gatgtccggc cgttgtacag ccgacggctg cccaagggcg tgaagcacct gaaggacttt 1560
ccgatcctgc cgggcgagat cttcaagtac aagtggactg tgaccgtgga ggatgggccg 1620
accaagagcg atccgcgctg cctgacccgt tactactcca gctttgtcaa tatggagcgc 1680
gacctcgcta gcggcttgat tggccctctg ctgatctgct acaaggagtc cgtggaccag 1740
agggggaatc agatcatgag tgacaagagg aacgtgatcc tgttctccgt gttcgacgaa 1800
aaccgcagct ggtatctcac cgagaatatc cagcgcttcc tgcccaaccc ggccggtgtg 1860
cagctggagg accccgagtt tcaggccagc aacatcatgc attctatcaa cggatatgtg 1920
tttgattccc tgcagctctc agtgtgtctg cacgaggtcg cctactggta tatcctcagc 1980
attggggcac agaccgactt cctgagcgtg ttcttctccg ggtatacctt caagcacaag 2040
atggtgtacg aggataccct gaccctgttc ccctttagcg gcgaaaccgt gtttatgtct 2100
atggagaacc ccgggctctg gatccttgca tgccataact ccgacttccg caaccgcgga 2160
atgaccgcgc tcctgaaagt gtcgagttgt gacaagaaca ccggcgacta ttacgaggac 2220
agttacgagg acatctctgc gtacctcctt agcaagaata acgccatcga gccaagatcc 2280
ttcagccaga accccccagt gctgaaggag atcacccgca cgaccctgca gtcggatcag 2340
gaggagattg attacgacga cacgatcagt gtggagatga agaaggagga cttcgacatc 2400
tacgacgaag atgaaaacca gtcccctcgg tccttccaaa agaagacccg gcactacttc 2460
atcgccgctg tggaacgcct gtgggactat ggaatgtctt ctagccctca cgttttgagg 2520
aaccgcgccc agtcgggcag cgtgccccag ttcaagaaag tggtgttcca ggagttcacc 2580
gacggctcct tcacccagcc actttaccgg ggcgagctca atgaacatct gggcctgctg 2640
ggaccctaca tcagggctga ggtggaggac aacatcatgg tgacattccg gaatcaggcc 2700
agcagaccat acagtttcta cagttcactc atctcctacg aggaggacca gcgccagggg 2760
gctgaacccc gtaagaactt cgtgaagcca aacgaaacaa agacctactt ctggaaggtc 2820
cagcaccaca tggcacctac caaggacgag ttcgattgca aggcctgggc ctacttctcc 2880
gacgtggacc tggagaaaga tgtgcacagc ggcctgattg gccctctgct ggtgtgtcac 2940
acgaacacac tcaaccctgc acacgggcgg caggtcactg tgcaggaatt cgccctgttc 3000
tttaccatct ttgatgagac gaagtcctgg tatttcaccg aaaacatgga gaggaactgc 3060
cgcgcaccct gcaacatcca gatggaagat ccgacattca aggagaacta ccggttccat 3120
gccatcaatg gctacatcat ggacaccctg cctggcctcg tgatggccca agaccagcgt 3180
atccgctggt atctgctgtc gatgggctcc aacgagaaca tccatagtat ccacttcagc 3240
gggcatgtct tcacggtgag gaaaaaggag gagtacaaga tggcactgta caacctctat 3300
cccggcgtgt tcgagaccgt ggagatgctg ccctccaagg ccggcatctg gagagtggaa 3360
tgcctgatcg gcgagcacct ccacgctggg atgtccacgc tgttcctcgt ttacagcaat 3420
aagtgccaga cccctctggg catggcgagc ggccacatcc gcgacttcca gattacagcc 3480
agcggccagt acggtcagtg ggctccaaag ctggcccgtc tgcactactc cggatccatc 3540
aacgcctggt ccaccaagga accgttctcc tggatcaaag tagacctgct agcccccatg 3600
atcattcacg gcatcaagac acaaggcgcc cgacagaagt tctcgagcct ctatatctcc 3660
cagttcatca tcatgtatag cctggacgga aagaagtggc agacttaccg cggaaactcg 3720
acagggaccc tgatggtatt cttcggtaac gtggacagct ccggaatcaa gcacaacatc 3780
ttcaacccac ccattatcgc ccgctacatc cgcctgcacc ccactcacta tagcattagg 3840
tccaccctgc gaatggagct catgggctgt gacctgaaca gctgtagcat gcccctcggc 3900
atggagtcta aggcgatctc cgacgcacag ataacggcat catcctactt taccaacatg 3960
ttcgctacct ggtccccctc caaggcccga ctccacctgc aagggagatc caacgcctgg 4020
cggccacagg tcaacaatcc caaggagtgg ctgcaagtgg actttcagaa aactatgaaa 4080
gtcaccggag tgaccacaca gggagtgaag tctctgctga ccagcatgta cgtgaaggag 4140
ttcctcatct ccagttcgca ggatggccac cagtggacgt tgttcttcca aaacggtaaa 4200
gtcaaagtct tccaagggaa ccaggacagc tttacacccg tcgtgaactc cctggacccc 4260
ccgcttctca ctagatacct ccgcatccac cctcagagct gggtgcacca gattgccctg 4320
cgcatggagg ttctggggtg tgaagcccag gacctgtact aa 4362
<210> 20
<211> 4362
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 20
atgcagatcg aactgagcac ttgcttcttc ctgtgtctcc tgcgcttttg cttctccgcc 60
acaaggagat actatctcgg tgccgtggag ctcagctggg actacatgca gagcgacttg 120
ggtgaactgc ctgtggacgc caggtttcca ccccgcgtgc ccaagagttt cccgttcaac 180
accagtgtcg tgtacaagaa aaccctcttc gtggaattca ccgaccacct gttcaacatc 240
gccaaaccgc gccctccctg gatggggctg ctcggcccga cgatccaggc tgaggtctat 300
gacacggtgg tgattaccct caagaacatg gctagccacc cggtgagcct gcacgccgtg 360
ggcgtgtcct attggaaagc gtccgagggt gcggagtacg atgaccagac ttcacagcgg 420
gagaaggaag acgacaaagt gttccccggg ggttcccaca cctatgtctg gcaggtcctg 480
aaggagaatg gtcctatggc ctccgaccca ttgtgcctca cctactctta cctaagccat 540
gtggatctcg tcaaggacct gaactcgggg ctgatcggcg ccctgctcgt gtgccgggag 600
ggctcactgg ccaaggagaa gacccaaact ctgcacaagt tcatcctgct gttcgcggta 660
ttcgacgagg ggaagtcctg gcactccgag accaagaaca gcctgatgca ggaccgcgac 720
gcagcctcgg cccgtgcgtg gccaaagatg cacaccgtga acggctacgt taacaggagc 780
ctacccggcc tgatcggctg ccaccgcaaa tcggtctact ggcatgtgat cggaatgggc 840
acaacgcccg aggtccacag tatcttcctc gagggccaca ctttcctggt ccggaatcac 900
cgccaggcca gcctggagat cagccccata acctttctga cggcgcagac cttactcatg 960
gatctcggcc agttcctcct gttctgccac atttcgtccc accagcacga tgggatggaa 1020
gcatatgtga aagtggactc ctgccccgag gaaccccagc ttaggatgaa gaacaatgag 1080
gaggccgagg actacgacga tgaccttacc gattcagaaa tggacgtagt acgctttgac 1140
gacgacaact ctccatcctt catacagatt cgctccgtcg ccaagaagca ccctaagact 1200
tgggtgcact acatcgcggc cgaggaggag gactgggatt atgctcccct ggtgctggcc 1260
cccgacgacc gcagctacaa gagccagtac ctgaataacg ggccccagcg catcggccgg 1320
aagtacaaga aagtgcggtt catggcttac acggacgaga ccttcaagac ccgggaggct 1380
atccagcatg agagcggcat cttggggccc ctcctgtacg gcgaagttgg agacacactg 1440
ctgatcatct tcaagaacca ggcgagcagg ccctacaaca tctaccccca cggcattacc 1500
gatgtccggc cgttgtacag ccgacggctg cccaagggcg tgaagcacct gaaggacttt 1560
ccgatcctgc cgggcgagat cttcaagtac aagtggactg tgaccgtgga ggatgggccg 1620
accaagagcg atccgcgctg cctgacccgt tactactcca gctttgtcaa tatggagcgc 1680
gacctcgcta gcggcttgat tggccctctg ctgatctgct acaaggagtc cgtggaccag 1740
agggggaatc agatcatgag tgacaagagg aacgtgatcc tgttctccgt gttcgacgaa 1800
aaccgcagct ggtatctcac cgagaatatc cagcgcttcc tgcccaaccc ggccggtgtg 1860
cagctggagg accccgagtt tcaggccagc aacatcatgc attctatcaa cggatatgtg 1920
tttgattccc tgcagctctc agtgtgtctg cacgaggtcg cctactggta tatcctcagc 1980
attggggcac agaccgactt cctgagcgtg ttcttctccg ggtatacctt caagcacaag 2040
atggtgtacg aggataccct gaccctgttc ccctttagcg gcgaaaccgt gtttatgtct 2100
atggagaacc ccgggctctg gatccttgcg tgccataact ccgacttccg caaccgcgga 2160
atgaccgcgc tcctgaaagt gtcgagttgt gacaagaaca ccggcgacta ttacgaggac 2220
agttacgagg acatctctgc gtacctcctt agcaagaata acgccatcga gccaagatcc 2280
ttcagccaga accccccagt gctgaaggag atcacccgca cgaccctgca gtcggatcag 2340
gaggagattg attacgacga cacgatcagt gtggagatga agaaggagga cttcgacatc 2400
tacgacgaag atgaaaacca gtcccctcgg tccttccaaa agaagacccg gcactacttc 2460
atcgccgctg tggaacgcct gtgggactat ggaatgtctt ctagccctca cgttttgagg 2520
aaccgcgccc agtcgggcag cgtgccccag ttcaagaaag tggtgttcca ggagttcacc 2580
gacggctcct tcacccagcc actttaccgg ggcgagctca atgaacatct gggcctgctg 2640
ggaccctaca tcagggctga ggtggaggac aacatcatgg tgacattccg gaatcaggcc 2700
agcagaccat acagtttcta cagttcactc atctcctacg aggaggacca gcgccagggg 2760
gctgaacccc gtaagaactt cgtgaagcca aacgaaacaa agacctactt ctggaaggtc 2820
cagcaccaca tggcacctac caaggacgag ttcgattgca aggcctgggc ctacttctcc 2880
gacgtggacc tggagaaaga tgtgcacagc ggcctgattg gccctctgct ggtgtgtcac 2940
acgaacacac tcaaccctgc acacgggcgg caggtcactg tgcaggaatt cgccctgttc 3000
tttaccatct ttgatgagac gaagtcctgg tatttcaccg aaaacatgga gaggaactgc 3060
cgcgcaccct gcaacatcca gatggaagat ccgacattca aggagaacta ccggttccat 3120
gccatcaatg gctacatcat ggacaccctg cctggcctcg tgatggccca agaccagcgt 3180
atccgctggt atctgctgtc gatgggctcc aacgagaaca tccatagtat ccacttcagc 3240
gggcatgtct tcacggtgag gaaaaaggag gagtacaaga tggcactgta caacctctat 3300
cccggcgtgt tcgagaccgt ggagatgctg ccctccaagg ccggcatctg gagagtggaa 3360
tgcctgatcg gcgagcacct ccacgctggg atgtccacgc tgttcctcgt ttacagcaat 3420
aagtgccaga cccctctggg catggcgagc ggccacatcc gcgacttcca gattacagcc 3480
agcggccagt acggtcagtg ggctccaaag ctggcccgtc tgcactactc cggatccatc 3540
aacgcctggt ccaccaagga accgttctcc tggatcaaag tagacctgct agcccccatg 3600
atcattcacg gcatcaagac acaaggcgcc cgacagaagt tctcgagcct ctatatctcc 3660
cagttcatca tcatgtatag cctggacgga aagaagtggc agacttaccg cggaaactcg 3720
acagggaccc tgatggtatt cttcggtaac gtggacagct ccggaatcaa gcacaacatc 3780
ttcaacccac ccattatcgc ccgctacatc cgcctgcacc ccactcacta tagcattagg 3840
tccaccctgc gaatggagct catgggctgt gacctgaaca gctgtagcat gcccctcggc 3900
atggagtcta aggcgatctc cgacgcacag ataacggcat catcctactt taccaacatg 3960
ttcgctacct ggtccccctc caaggcccga ctccacctgc aagggagatc caacgcctgg 4020
cggccacagg tcaacaatcc caaggagtgg ctgcaagtgg actttcagaa aactatgaaa 4080
gtcaccggag tgaccacaca gggagtgaag tctctgctga ccagcatgta cgtgaaggag 4140
ttcctcatct ccagttcgca ggatggccac cagtggacgt tgttcttcca aaacggtaaa 4200
gtcaaagtct tccaagggaa ccaggacagc tttacacccg tcgtgaactc cctggacccc 4260
ccgcttctca ctagatacct ccgcatccac cctcagagct gggtgcacca gattgccctg 4320
cgcatggagg ttctggggtg tgaagcccag gacctgtact aa 4362
<210> 21
<211> 2351
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 21
Met Gln Ile Glu Leu Ser Thr Cys Phe Phe Leu Cys Leu Leu Arg Phe
1 5 10 15
Cys Phe Ser Ala Thr Arg Arg Tyr Tyr Leu Gly Ala Val Glu Leu Ser
20 25 30
Trp Asp Tyr Met Gln Ser Asp Leu Gly Glu Leu Pro Val Asp Ala Arg
35 40 45
Phe Pro Pro Arg Val Pro Lys Ser Phe Pro Phe Asn Thr Ser Val Val
50 55 60
Tyr Lys Lys Thr Leu Phe Val Glu Phe Thr Asp His Leu Phe Asn Ile
65 70 75 80
Ala Lys Pro Arg Pro Pro Trp Met Gly Leu Leu Gly Pro Thr Ile Gln
85 90 95
Ala Glu Val Tyr Asp Thr Val Val Ile Thr Leu Lys Asn Met Ala Ser
100 105 110
His Pro Val Ser Leu His Ala Val Gly Val Ser Tyr Trp Lys Ala Ser
115 120 125
Glu Gly Ala Glu Tyr Asp Asp Gln Thr Ser Gln Arg Glu Lys Glu Asp
130 135 140
Asp Lys Val Phe Pro Gly Gly Ser His Thr Tyr Val Trp Gln Val Leu
145 150 155 160
Lys Glu Asn Gly Pro Met Ala Ser Asp Pro Leu Cys Leu Thr Tyr Ser
165 170 175
Tyr Leu Ser His Val Asp Leu Val Lys Asp Leu Asn Ser Gly Leu Ile
180 185 190
Gly Ala Leu Leu Val Cys Arg Glu Gly Ser Leu Ala Lys Glu Lys Thr
195 200 205
Gln Thr Leu His Lys Phe Ile Leu Leu Phe Ala Val Phe Asp Glu Gly
210 215 220
Lys Ser Trp His Ser Glu Thr Lys Asn Ser Leu Met Gln Asp Arg Asp
225 230 235 240
Ala Ala Ser Ala Arg Ala Trp Pro Lys Met His Thr Val Asn Gly Tyr
245 250 255
Val Asn Arg Ser Leu Pro Gly Leu Ile Gly Cys His Arg Lys Ser Val
260 265 270
Tyr Trp His Val Ile Gly Met Gly Thr Thr Pro Glu Val His Ser Ile
275 280 285
Phe Leu Glu Gly His Thr Phe Leu Val Arg Asn His Arg Gln Ala Ser
290 295 300
Leu Glu Ile Ser Pro Ile Thr Phe Leu Thr Ala Gln Thr Leu Leu Met
305 310 315 320
Asp Leu Gly Gln Phe Leu Leu Phe Cys His Ile Ser Ser His Gln His
325 330 335
Asp Gly Met Glu Ala Tyr Val Lys Val Asp Ser Cys Pro Glu Glu Pro
340 345 350
Gln Leu Arg Met Lys Asn Asn Glu Glu Ala Glu Asp Tyr Asp Asp Asp
355 360 365
Leu Thr Asp Ser Glu Met Asp Val Val Arg Phe Asp Asp Asp Asn Ser
370 375 380
Pro Ser Phe Ile Gln Ile Arg Ser Val Ala Lys Lys His Pro Lys Thr
385 390 395 400
Trp Val His Tyr Ile Ala Ala Glu Glu Glu Asp Trp Asp Tyr Ala Pro
405 410 415
Leu Val Leu Ala Pro Asp Asp Arg Ser Tyr Lys Ser Gln Tyr Leu Asn
420 425 430
Asn Gly Pro Gln Arg Ile Gly Arg Lys Tyr Lys Lys Val Arg Phe Met
435 440 445
Ala Tyr Thr Asp Glu Thr Phe Lys Thr Arg Glu Ala Ile Gln His Glu
450 455 460
Ser Gly Ile Leu Gly Pro Leu Leu Tyr Gly Glu Val Gly Asp Thr Leu
465 470 475 480
Leu Ile Ile Phe Lys Asn Gln Ala Ser Arg Pro Tyr Asn Ile Tyr Pro
485 490 495
His Gly Ile Thr Asp Val Arg Pro Leu Tyr Ser Arg Arg Leu Pro Lys
500 505 510
Gly Val Lys His Leu Lys Asp Phe Pro Ile Leu Pro Gly Glu Ile Phe
515 520 525
Lys Tyr Lys Trp Thr Val Thr Val Glu Asp Gly Pro Thr Lys Ser Asp
530 535 540
Pro Arg Cys Leu Thr Arg Tyr Tyr Ser Ser Phe Val Asn Met Glu Arg
545 550 555 560
Asp Leu Ala Ser Gly Leu Ile Gly Pro Leu Leu Ile Cys Tyr Lys Glu
565 570 575
Ser Val Asp Gln Arg Gly Asn Gln Ile Met Ser Asp Lys Arg Asn Val
580 585 590
Ile Leu Phe Ser Val Phe Asp Glu Asn Arg Ser Trp Tyr Leu Thr Glu
595 600 605
Asn Ile Gln Arg Phe Leu Pro Asn Pro Ala Gly Val Gln Leu Glu Asp
610 615 620
Pro Glu Phe Gln Ala Ser Asn Ile Met His Ser Ile Asn Gly Tyr Val
625 630 635 640
Phe Asp Ser Leu Gln Leu Ser Val Cys Leu His Glu Val Ala Tyr Trp
645 650 655
Tyr Ile Leu Ser Ile Gly Ala Gln Thr Asp Phe Leu Ser Val Phe Phe
660 665 670
Ser Gly Tyr Thr Phe Lys His Lys Met Val Tyr Glu Asp Thr Leu Thr
675 680 685
Leu Phe Pro Phe Ser Gly Glu Thr Val Phe Met Ser Met Glu Asn Pro
690 695 700
Gly Leu Trp Ile Leu Ala Cys His Asn Ser Asp Phe Arg Asn Arg Gly
705 710 715 720
Met Thr Ala Leu Leu Lys Val Ser Ser Cys Asp Lys Asn Thr Gly Asp
725 730 735
Tyr Tyr Glu Asp Ser Tyr Glu Asp Ile Ser Ala Tyr Leu Leu Ser Lys
740 745 750
Asn Asn Ala Ile Glu Pro Arg Ser Phe Ser Gln Asn Ser Arg His Pro
755 760 765
Ser Thr Arg Gln Lys Gln Phe Asn Ala Thr Thr Ile Pro Glu Asn Asp
770 775 780
Ile Glu Lys Thr Asp Pro Trp Phe Ala His Arg Thr Pro Met Pro Lys
785 790 795 800
Ile Gln Asn Val Ser Ser Ser Asp Leu Leu Met Leu Leu Arg Gln Ser
805 810 815
Pro Thr Pro His Gly Leu Ser Leu Ser Asp Leu Gln Glu Ala Lys Tyr
820 825 830
Glu Thr Phe Ser Asp Asp Pro Ser Pro Gly Ala Ile Asp Ser Asn Asn
835 840 845
Ser Leu Ser Glu Met Thr His Phe Arg Pro Gln Leu His His Ser Gly
850 855 860
Asp Met Val Phe Thr Pro Glu Ser Gly Leu Gln Leu Arg Leu Asn Glu
865 870 875 880
Lys Leu Gly Thr Thr Ala Ala Thr Glu Leu Lys Lys Leu Asp Phe Lys
885 890 895
Val Ser Ser Thr Ser Asn Asn Leu Ile Ser Thr Ile Pro Ser Asp Asn
900 905 910
Leu Ala Ala Gly Thr Asp Asn Thr Ser Ser Leu Gly Pro Pro Ser Met
915 920 925
Pro Val His Tyr Asp Ser Gln Leu Asp Thr Thr Leu Phe Gly Lys Lys
930 935 940
Ser Ser Pro Leu Thr Glu Ser Gly Gly Pro Leu Ser Leu Ser Glu Glu
945 950 955 960
Asn Asn Asp Ser Lys Leu Leu Glu Ser Gly Leu Met Asn Ser Gln Glu
965 970 975
Ser Ser Trp Gly Lys Asn Val Ser Ser Thr Glu Ser Gly Arg Leu Phe
980 985 990
Lys Gly Lys Arg Ala His Gly Pro Ala Leu Leu Thr Lys Asp Asn Ala
995 1000 1005
Leu Phe Lys Val Ser Ile Ser Leu Leu Lys Thr Asn Lys Thr Ser Asn
1010 1015 1020
Asn Ser Ala Thr Asn Arg Lys Thr His Ile Asp Gly Pro Ser Leu Leu
1025 1030 1035 1040
Ile Glu Asn Ser Pro Ser Val Trp Gln Asn Ile Leu Glu Ser Asp Thr
1045 1050 1055
Glu Phe Lys Lys Val Thr Pro Leu Ile His Asp Arg Met Leu Met Asp
1060 1065 1070
Lys Asn Ala Thr Ala Leu Arg Leu Asn His Met Ser Asn Lys Thr Thr
1075 1080 1085
Ser Ser Lys Asn Met Glu Met Val Gln Gln Lys Lys Glu Gly Pro Ile
1090 1095 1100
Pro Pro Asp Ala Gln Asn Pro Asp Met Ser Phe Phe Lys Met Leu Phe
1105 1110 1115 1120
Leu Pro Glu Ser Ala Arg Trp Ile Gln Arg Thr His Gly Lys Asn Ser
1125 1130 1135
Leu Asn Ser Gly Gln Gly Pro Ser Pro Lys Gln Leu Val Ser Leu Gly
1140 1145 1150
Pro Glu Lys Ser Val Glu Gly Gln Asn Phe Leu Ser Glu Lys Asn Lys
1155 1160 1165
Val Val Val Gly Lys Gly Glu Phe Thr Lys Asp Val Gly Leu Lys Glu
1170 1175 1180
Met Val Phe Pro Ser Ser Arg Asn Leu Phe Leu Thr Asn Leu Asp Asn
1185 1190 1195 1200
Leu His Glu Asn Asn Thr His Asn Gln Glu Lys Lys Ile Gln Glu Glu
1205 1210 1215
Ile Glu Lys Lys Glu Thr Leu Ile Gln Glu Asn Val Val Leu Pro Gln
1220 1225 1230
Ile His Thr Val Thr Gly Thr Lys Asn Phe Met Lys Asn Leu Phe Leu
1235 1240 1245
Leu Ser Thr Arg Gln Asn Val Glu Gly Ser Tyr Asp Gly Ala Tyr Ala
1250 1255 1260
Pro Val Leu Gln Asp Phe Arg Ser Leu Asn Asp Ser Thr Asn Arg Thr
1265 1270 1275 1280
Lys Lys His Thr Ala His Phe Ser Lys Lys Gly Glu Glu Glu Asn Leu
1285 1290 1295
Glu Gly Leu Gly Asn Gln Thr Lys Gln Ile Val Glu Lys Tyr Ala Cys
1300 1305 1310
Thr Thr Arg Ile Ser Pro Asn Thr Ser Gln Gln Asn Phe Val Thr Gln
1315 1320 1325
Arg Ser Lys Arg Ala Leu Lys Gln Phe Arg Leu Pro Leu Glu Glu Thr
1330 1335 1340
Glu Leu Glu Lys Arg Ile Ile Val Asp Asp Thr Ser Thr Gln Trp Ser
1345 1350 1355 1360
Lys Asn Met Lys His Leu Thr Pro Ser Thr Leu Thr Gln Ile Asp Tyr
1365 1370 1375
Asn Glu Lys Glu Lys Gly Ala Ile Thr Gln Ser Pro Leu Ser Asp Cys
1380 1385 1390
Leu Thr Arg Ser His Ser Ile Pro Gln Ala Asn Arg Ser Pro Leu Pro
1395 1400 1405
Ile Ala Lys Val Ser Ser Phe Pro Ser Ile Arg Pro Ile Tyr Leu Thr
1410 1415 1420
Arg Val Leu Phe Gln Asp Asn Ser Ser His Leu Pro Ala Ala Ser Tyr
1425 1430 1435 1440
Arg Lys Lys Asp Ser Gly Val Gln Glu Ser Ser His Phe Leu Gln Gly
1445 1450 1455
Ala Lys Lys Asn Asn Leu Ser Leu Ala Ile Leu Thr Leu Glu Met Thr
1460 1465 1470
Gly Asp Gln Arg Glu Val Gly Ser Leu Gly Thr Ser Ala Thr Asn Ser
1475 1480 1485
Val Thr Tyr Lys Lys Val Glu Asn Thr Val Leu Pro Lys Pro Asp Leu
1490 1495 1500
Pro Lys Thr Ser Gly Lys Val Glu Leu Leu Pro Lys Val His Ile Tyr
1505 1510 1515 1520
Gln Lys Asp Leu Phe Pro Thr Glu Thr Ser Asn Gly Ser Pro Gly His
1525 1530 1535
Leu Asp Leu Val Glu Gly Ser Leu Leu Gln Gly Thr Glu Gly Ala Ile
1540 1545 1550
Lys Trp Asn Glu Ala Asn Arg Pro Gly Lys Val Pro Phe Leu Arg Val
1555 1560 1565
Ala Thr Glu Ser Ser Ala Lys Thr Pro Ser Lys Leu Leu Asp Pro Leu
1570 1575 1580
Ala Trp Asp Asn His Tyr Gly Thr Gln Ile Pro Lys Glu Glu Trp Lys
1585 1590 1595 1600
Ser Gln Glu Lys Ser Pro Glu Lys Thr Ala Phe Lys Lys Lys Asp Thr
1605 1610 1615
Ile Leu Ser Leu Asn Ala Cys Glu Ser Asn His Ala Ile Ala Ala Ile
1620 1625 1630
Asn Glu Gly Gln Asn Lys Pro Glu Ile Glu Val Thr Trp Ala Lys Gln
1635 1640 1645
Gly Arg Thr Glu Arg Leu Cys Ser Gln Asn Pro Pro Val Leu Lys Arg
1650 1655 1660
His Gln Arg Glu Ile Thr Arg Thr Thr Leu Gln Ser Asp Gln Glu Glu
1665 1670 1675 1680
Ile Asp Tyr Asp Asp Thr Ile Ser Val Glu Met Lys Lys Glu Asp Phe
1685 1690 1695
Asp Ile Tyr Asp Glu Asp Glu Asn Gln Ser Pro Arg Ser Phe Gln Lys
1700 1705 1710
Lys Thr Arg His Tyr Phe Ile Ala Ala Val Glu Arg Leu Trp Asp Tyr
1715 1720 1725
Gly Met Ser Ser Ser Pro His Val Leu Arg Asn Arg Ala Gln Ser Gly
1730 1735 1740
Ser Val Pro Gln Phe Lys Lys Val Val Phe Gln Glu Phe Thr Asp Gly
1745 1750 1755 1760
Ser Phe Thr Gln Pro Leu Tyr Arg Gly Glu Leu Asn Glu His Leu Gly
1765 1770 1775
Leu Leu Gly Pro Tyr Ile Arg Ala Glu Val Glu Asp Asn Ile Met Val
1780 1785 1790
Thr Phe Arg Asn Gln Ala Ser Arg Pro Tyr Ser Phe Tyr Ser Ser Leu
1795 1800 1805
Ile Ser Tyr Glu Glu Asp Gln Arg Gln Gly Ala Glu Pro Arg Lys Asn
1810 1815 1820
Phe Val Lys Pro Asn Glu Thr Lys Thr Tyr Phe Trp Lys Val Gln His
1825 1830 1835 1840
His Met Ala Pro Thr Lys Asp Glu Phe Asp Cys Lys Ala Trp Ala Tyr
1845 1850 1855
Phe Ser Asp Val Asp Leu Glu Lys Asp Val His Ser Gly Leu Ile Gly
1860 1865 1870
Pro Leu Leu Val Cys His Thr Asn Thr Leu Asn Pro Ala His Gly Arg
1875 1880 1885
Gln Val Thr Val Gln Glu Phe Ala Leu Phe Phe Thr Ile Phe Asp Glu
1890 1895 1900
Thr Lys Ser Trp Tyr Phe Thr Glu Asn Met Glu Arg Asn Cys Arg Ala
1905 1910 1915 1920
Pro Cys Asn Ile Gln Met Glu Asp Pro Thr Phe Lys Glu Asn Tyr Arg
1925 1930 1935
Phe His Ala Ile Asn Gly Tyr Ile Met Asp Thr Leu Pro Gly Leu Val
1940 1945 1950
Met Ala Gln Asp Gln Arg Ile Arg Trp Tyr Leu Leu Ser Met Gly Ser
1955 1960 1965
Asn Glu Asn Ile His Ser Ile His Phe Ser Gly His Val Phe Thr Val
1970 1975 1980
Arg Lys Lys Glu Glu Tyr Lys Met Ala Leu Tyr Asn Leu Tyr Pro Gly
1985 1990 1995 2000
Val Phe Glu Thr Val Glu Met Leu Pro Ser Lys Ala Gly Ile Trp Arg
2005 2010 2015
Val Glu Cys Leu Ile Gly Glu His Leu His Ala Gly Met Ser Thr Leu
2020 2025 2030
Phe Leu Val Tyr Ser Asn Lys Cys Gln Thr Pro Leu Gly Met Ala Ser
2035 2040 2045
Gly His Ile Arg Asp Phe Gln Ile Thr Ala Ser Gly Gln Tyr Gly Gln
2050 2055 2060
Trp Ala Pro Lys Leu Ala Arg Leu His Tyr Ser Gly Ser Ile Asn Ala
2065 2070 2075 2080
Trp Ser Thr Lys Glu Pro Phe Ser Trp Ile Lys Val Asp Leu Leu Ala
2085 2090 2095
Pro Met Ile Ile His Gly Ile Lys Thr Gln Gly Ala Arg Gln Lys Phe
2100 2105 2110
Ser Ser Leu Tyr Ile Ser Gln Phe Ile Ile Met Tyr Ser Leu Asp Gly
2115 2120 2125
Lys Lys Trp Gln Thr Tyr Arg Gly Asn Ser Thr Gly Thr Leu Met Val
2130 2135 2140
Phe Phe Gly Asn Val Asp Ser Ser Gly Ile Lys His Asn Ile Phe Asn
2145 2150 2155 2160
Pro Pro Ile Ile Ala Arg Tyr Ile Arg Leu His Pro Thr His Tyr Ser
2165 2170 2175
Ile Arg Ser Thr Leu Arg Met Glu Leu Met Gly Cys Asp Leu Asn Ser
2180 2185 2190
Cys Ser Met Pro Leu Gly Met Glu Ser Lys Ala Ile Ser Asp Ala Gln
2195 2200 2205
Ile Thr Ala Ser Ser Tyr Phe Thr Asn Met Phe Ala Thr Trp Ser Pro
2210 2215 2220
Ser Lys Ala Arg Leu His Leu Gln Gly Arg Ser Asn Ala Trp Arg Pro
2225 2230 2235 2240
Gln Val Asn Asn Pro Lys Glu Trp Leu Gln Val Asp Phe Gln Lys Thr
2245 2250 2255
Met Lys Val Thr Gly Val Thr Thr Gln Gly Val Lys Ser Leu Leu Thr
2260 2265 2270
Ser Met Tyr Val Lys Glu Phe Leu Ile Ser Ser Ser Gln Asp Gly His
2275 2280 2285
Gln Trp Thr Leu Phe Phe Gln Asn Gly Lys Val Lys Val Phe Gln Gly
2290 2295 2300
Asn Gln Asp Ser Phe Thr Pro Val Val Asn Ser Leu Asp Pro Pro Leu
2305 2310 2315 2320
Leu Thr Arg Tyr Leu Arg Ile His Pro Gln Ser Trp Val His Gln Ile
2325 2330 2335
Ala Leu Arg Met Glu Val Leu Gly Cys Glu Ala Gln Asp Leu Tyr
2340 2345 2350
<210> 22
<211> 1457
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 22
Met Gln Ile Glu Leu Ser Thr Cys Phe Phe Leu Cys Leu Leu Arg Phe
1 5 10 15
Cys Phe Ser Ala Thr Arg Arg Tyr Tyr Leu Gly Ala Val Glu Leu Ser
20 25 30
Trp Asp Tyr Met Gln Ser Asp Leu Gly Glu Leu Pro Val Asp Ala Arg
35 40 45
Phe Pro Pro Arg Val Pro Lys Ser Phe Pro Phe Asn Thr Ser Val Val
50 55 60
Tyr Lys Lys Thr Leu Phe Val Glu Phe Thr Asp His Leu Phe Asn Ile
65 70 75 80
Ala Lys Pro Arg Pro Pro Trp Met Gly Leu Leu Gly Pro Thr Ile Gln
85 90 95
Ala Glu Val Tyr Asp Thr Val Val Ile Thr Leu Lys Asn Met Ala Ser
100 105 110
His Pro Val Ser Leu His Ala Val Gly Val Ser Tyr Trp Lys Ala Ser
115 120 125
Glu Gly Ala Glu Tyr Asp Asp Gln Thr Ser Gln Arg Glu Lys Glu Asp
130 135 140
Asp Lys Val Phe Pro Gly Gly Ser His Thr Tyr Val Trp Gln Val Leu
145 150 155 160
Lys Glu Asn Gly Pro Met Ala Ser Asp Pro Leu Cys Leu Thr Tyr Ser
165 170 175
Tyr Leu Ser His Val Asp Leu Val Lys Asp Leu Asn Ser Gly Leu Ile
180 185 190
Gly Ala Leu Leu Val Cys Arg Glu Gly Ser Leu Ala Lys Glu Lys Thr
195 200 205
Gln Thr Leu His Lys Phe Ile Leu Leu Phe Ala Val Phe Asp Glu Gly
210 215 220
Lys Ser Trp His Ser Glu Thr Lys Asn Ser Leu Met Gln Asp Arg Asp
225 230 235 240
Ala Ala Ser Ala Arg Ala Trp Pro Lys Met His Thr Val Asn Gly Tyr
245 250 255
Val Asn Arg Ser Leu Pro Gly Leu Ile Gly Cys His Arg Lys Ser Val
260 265 270
Tyr Trp His Val Ile Gly Met Gly Thr Thr Pro Glu Val His Ser Ile
275 280 285
Phe Leu Glu Gly His Thr Phe Leu Val Arg Asn His Arg Gln Ala Ser
290 295 300
Leu Glu Ile Ser Pro Ile Thr Phe Leu Thr Ala Gln Thr Leu Leu Met
305 310 315 320
Asp Leu Gly Gln Phe Leu Leu Phe Cys His Ile Ser Ser His Gln His
325 330 335
Asp Gly Met Glu Ala Tyr Val Lys Val Asp Ser Cys Pro Glu Glu Pro
340 345 350
Gln Leu Arg Met Lys Asn Asn Glu Glu Ala Glu Asp Tyr Asp Asp Asp
355 360 365
Leu Thr Asp Ser Glu Met Asp Val Val Arg Phe Asp Asp Asp Asn Ser
370 375 380
Pro Ser Phe Ile Gln Ile Arg Ser Val Ala Lys Lys His Pro Lys Thr
385 390 395 400
Trp Val His Tyr Ile Ala Ala Glu Glu Glu Asp Trp Asp Tyr Ala Pro
405 410 415
Leu Val Leu Ala Pro Asp Asp Arg Ser Tyr Lys Ser Gln Tyr Leu Asn
420 425 430
Asn Gly Pro Gln Arg Ile Gly Arg Lys Tyr Lys Lys Val Arg Phe Met
435 440 445
Ala Tyr Thr Asp Glu Thr Phe Lys Thr Arg Glu Ala Ile Gln His Glu
450 455 460
Ser Gly Ile Leu Gly Pro Leu Leu Tyr Gly Glu Val Gly Asp Thr Leu
465 470 475 480
Leu Ile Ile Phe Lys Asn Gln Ala Ser Arg Pro Tyr Asn Ile Tyr Pro
485 490 495
His Gly Ile Thr Asp Val Arg Pro Leu Tyr Ser Arg Arg Leu Pro Lys
500 505 510
Gly Val Lys His Leu Lys Asp Phe Pro Ile Leu Pro Gly Glu Ile Phe
515 520 525
Lys Tyr Lys Trp Thr Val Thr Val Glu Asp Gly Pro Thr Lys Ser Asp
530 535 540
Pro Arg Cys Leu Thr Arg Tyr Tyr Ser Ser Phe Val Asn Met Glu Arg
545 550 555 560
Asp Leu Ala Ser Gly Leu Ile Gly Pro Leu Leu Ile Cys Tyr Lys Glu
565 570 575
Ser Val Asp Gln Arg Gly Asn Gln Ile Met Ser Asp Lys Arg Asn Val
580 585 590
Ile Leu Phe Ser Val Phe Asp Glu Asn Arg Ser Trp Tyr Leu Thr Glu
595 600 605
Asn Ile Gln Arg Phe Leu Pro Asn Pro Ala Gly Val Gln Leu Glu Asp
610 615 620
Pro Glu Phe Gln Ala Ser Asn Ile Met His Ser Ile Asn Gly Tyr Val
625 630 635 640
Phe Asp Ser Leu Gln Leu Ser Val Cys Leu His Glu Val Ala Tyr Trp
645 650 655
Tyr Ile Leu Ser Ile Gly Ala Gln Thr Asp Phe Leu Ser Val Phe Phe
660 665 670
Ser Gly Tyr Thr Phe Lys His Lys Met Val Tyr Glu Asp Thr Leu Thr
675 680 685
Leu Phe Pro Phe Ser Gly Glu Thr Val Phe Met Ser Met Glu Asn Pro
690 695 700
Gly Leu Trp Ile Leu Ala Cys His Asn Ser Asp Phe Arg Asn Arg Gly
705 710 715 720
Met Thr Ala Leu Leu Lys Val Ser Ser Cys Asp Lys Asn Thr Gly Asp
725 730 735
Tyr Tyr Glu Asp Ser Tyr Glu Asp Ile Ser Ala Tyr Leu Leu Ser Lys
740 745 750
Asn Asn Ala Ile Glu Pro Arg Ser Phe Ser Gln Asn Pro Pro Val Leu
755 760 765
Lys Arg His Gln Arg Glu Ile Thr Arg Thr Thr Leu Gln Ser Asp Gln
770 775 780
Glu Glu Ile Asp Tyr Asp Asp Thr Ile Ser Val Glu Met Lys Lys Glu
785 790 795 800
Asp Phe Asp Ile Tyr Asp Glu Asp Glu Asn Gln Ser Pro Arg Ser Phe
805 810 815
Gln Lys Lys Thr Arg His Tyr Phe Ile Ala Ala Val Glu Arg Leu Trp
820 825 830
Asp Tyr Gly Met Ser Ser Ser Pro His Val Leu Arg Asn Arg Ala Gln
835 840 845
Ser Gly Ser Val Pro Gln Phe Lys Lys Val Val Phe Gln Glu Phe Thr
850 855 860
Asp Gly Ser Phe Thr Gln Pro Leu Tyr Arg Gly Glu Leu Asn Glu His
865 870 875 880
Leu Gly Leu Leu Gly Pro Tyr Ile Arg Ala Glu Val Glu Asp Asn Ile
885 890 895
Met Val Thr Phe Arg Asn Gln Ala Ser Arg Pro Tyr Ser Phe Tyr Ser
900 905 910
Ser Leu Ile Ser Tyr Glu Glu Asp Gln Arg Gln Gly Ala Glu Pro Arg
915 920 925
Lys Asn Phe Val Lys Pro Asn Glu Thr Lys Thr Tyr Phe Trp Lys Val
930 935 940
Gln His His Met Ala Pro Thr Lys Asp Glu Phe Asp Cys Lys Ala Trp
945 950 955 960
Ala Tyr Phe Ser Asp Val Asp Leu Glu Lys Asp Val His Ser Gly Leu
965 970 975
Ile Gly Pro Leu Leu Val Cys His Thr Asn Thr Leu Asn Pro Ala His
980 985 990
Gly Arg Gln Val Thr Val Gln Glu Phe Ala Leu Phe Phe Thr Ile Phe
995 1000 1005
Asp Glu Thr Lys Ser Trp Tyr Phe Thr Glu Asn Met Glu Arg Asn Cys
1010 1015 1020
Arg Ala Pro Cys Asn Ile Gln Met Glu Asp Pro Thr Phe Lys Glu Asn
1025 1030 1035 1040
Tyr Arg Phe His Ala Ile Asn Gly Tyr Ile Met Asp Thr Leu Pro Gly
1045 1050 1055
Leu Val Met Ala Gln Asp Gln Arg Ile Arg Trp Tyr Leu Leu Ser Met
1060 1065 1070
Gly Ser Asn Glu Asn Ile His Ser Ile His Phe Ser Gly His Val Phe
1075 1080 1085
Thr Val Arg Lys Lys Glu Glu Tyr Lys Met Ala Leu Tyr Asn Leu Tyr
1090 1095 1100
Pro Gly Val Phe Glu Thr Val Glu Met Leu Pro Ser Lys Ala Gly Ile
1105 1110 1115 1120
Trp Arg Val Glu Cys Leu Ile Gly Glu His Leu His Ala Gly Met Ser
1125 1130 1135
Thr Leu Phe Leu Val Tyr Ser Asn Lys Cys Gln Thr Pro Leu Gly Met
1140 1145 1150
Ala Ser Gly His Ile Arg Asp Phe Gln Ile Thr Ala Ser Gly Gln Tyr
1155 1160 1165
Gly Gln Trp Ala Pro Lys Leu Ala Arg Leu His Tyr Ser Gly Ser Ile
1170 1175 1180
Asn Ala Trp Ser Thr Lys Glu Pro Phe Ser Trp Ile Lys Val Asp Leu
1185 1190 1195 1200
Leu Ala Pro Met Ile Ile His Gly Ile Lys Thr Gln Gly Ala Arg Gln
1205 1210 1215
Lys Phe Ser Ser Leu Tyr Ile Ser Gln Phe Ile Ile Met Tyr Ser Leu
1220 1225 1230
Asp Gly Lys Lys Trp Gln Thr Tyr Arg Gly Asn Ser Thr Gly Thr Leu
1235 1240 1245
Met Val Phe Phe Gly Asn Val Asp Ser Ser Gly Ile Lys His Asn Ile
1250 1255 1260
Phe Asn Pro Pro Ile Ile Ala Arg Tyr Ile Arg Leu His Pro Thr His
1265 1270 1275 1280
Tyr Ser Ile Arg Ser Thr Leu Arg Met Glu Leu Met Gly Cys Asp Leu
1285 1290 1295
Asn Ser Cys Ser Met Pro Leu Gly Met Glu Ser Lys Ala Ile Ser Asp
1300 1305 1310
Ala Gln Ile Thr Ala Ser Ser Tyr Phe Thr Asn Met Phe Ala Thr Trp
1315 1320 1325
Ser Pro Ser Lys Ala Arg Leu His Leu Gln Gly Arg Ser Asn Ala Trp
1330 1335 1340
Arg Pro Gln Val Asn Asn Pro Lys Glu Trp Leu Gln Val Asp Phe Gln
1345 1350 1355 1360
Lys Thr Met Lys Val Thr Gly Val Thr Thr Gln Gly Val Lys Ser Leu
1365 1370 1375
Leu Thr Ser Met Tyr Val Lys Glu Phe Leu Ile Ser Ser Ser Gln Asp
1380 1385 1390
Gly His Gln Trp Thr Leu Phe Phe Gln Asn Gly Lys Val Lys Val Phe
1395 1400 1405
Gln Gly Asn Gln Asp Ser Phe Thr Pro Val Val Asn Ser Leu Asp Pro
1410 1415 1420
Pro Leu Leu Thr Arg Tyr Leu Arg Ile His Pro Gln Ser Trp Val His
1425 1430 1435 1440
Gln Ile Ala Leu Arg Met Glu Val Leu Gly Cys Glu Ala Gln Asp Leu
1445 1450 1455
Tyr
<210> 23
<211> 1464
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 23
Met Gln Ile Glu Leu Ser Thr Cys Phe Phe Leu Cys Leu Leu Arg Phe
1 5 10 15
Cys Phe Ser Ala Thr Arg Arg Tyr Tyr Leu Gly Ala Val Glu Leu Ser
20 25 30
Trp Asp Tyr Met Gln Ser Asp Leu Gly Glu Leu Pro Val Asp Ala Arg
35 40 45
Phe Pro Pro Arg Val Pro Lys Ser Phe Pro Phe Asn Thr Ser Val Val
50 55 60
Tyr Lys Lys Thr Leu Phe Val Glu Phe Thr Asp His Leu Phe Asn Ile
65 70 75 80
Ala Lys Pro Arg Pro Pro Trp Met Gly Leu Leu Gly Pro Thr Ile Gln
85 90 95
Ala Glu Val Tyr Asp Thr Val Val Ile Thr Leu Lys Asn Met Ala Ser
100 105 110
His Pro Val Ser Leu His Ala Val Gly Val Ser Tyr Trp Lys Ala Ser
115 120 125
Glu Gly Ala Glu Tyr Asp Asp Gln Thr Ser Gln Arg Glu Lys Glu Asp
130 135 140
Asp Lys Val Phe Pro Gly Gly Ser His Thr Tyr Val Trp Gln Val Leu
145 150 155 160
Lys Glu Asn Gly Pro Met Ala Ser Asp Pro Leu Cys Leu Thr Tyr Ser
165 170 175
Tyr Leu Ser His Val Asp Leu Val Lys Asp Leu Asn Ser Gly Leu Ile
180 185 190
Gly Ala Leu Leu Val Cys Arg Glu Gly Ser Leu Ala Lys Glu Lys Thr
195 200 205
Gln Thr Leu His Lys Phe Ile Leu Leu Phe Ala Val Phe Asp Glu Gly
210 215 220
Lys Ser Trp His Ser Glu Thr Lys Asn Ser Leu Met Gln Asp Arg Asp
225 230 235 240
Ala Ala Ser Ala Arg Ala Trp Pro Lys Met His Thr Val Asn Gly Tyr
245 250 255
Val Asn Arg Ser Leu Pro Gly Leu Ile Gly Cys His Arg Lys Ser Val
260 265 270
Tyr Trp His Val Ile Gly Met Gly Thr Thr Pro Glu Val His Ser Ile
275 280 285
Phe Leu Glu Gly His Thr Phe Leu Val Arg Asn His Arg Gln Ala Ser
290 295 300
Leu Glu Ile Ser Pro Ile Thr Phe Leu Thr Ala Gln Thr Leu Leu Met
305 310 315 320
Asp Leu Gly Gln Phe Leu Leu Phe Cys His Ile Ser Ser His Gln His
325 330 335
Asp Gly Met Glu Ala Tyr Val Lys Val Asp Ser Cys Pro Glu Glu Pro
340 345 350
Gln Leu Arg Met Lys Asn Asn Glu Glu Ala Glu Asp Tyr Asp Asp Asp
355 360 365
Leu Thr Asp Ser Glu Met Asp Val Val Arg Phe Asp Asp Asp Asn Ser
370 375 380
Pro Ser Phe Ile Gln Ile Arg Ser Val Ala Lys Lys His Pro Lys Thr
385 390 395 400
Trp Val His Tyr Ile Ala Ala Glu Glu Glu Asp Trp Asp Tyr Ala Pro
405 410 415
Leu Val Leu Ala Pro Asp Asp Arg Ser Tyr Lys Ser Gln Tyr Leu Asn
420 425 430
Asn Gly Pro Gln Arg Ile Gly Arg Lys Tyr Lys Lys Val Arg Phe Met
435 440 445
Ala Tyr Thr Asp Glu Thr Phe Lys Thr Arg Glu Ala Ile Gln His Glu
450 455 460
Ser Gly Ile Leu Gly Pro Leu Leu Tyr Gly Glu Val Gly Asp Thr Leu
465 470 475 480
Leu Ile Ile Phe Lys Asn Gln Ala Ser Arg Pro Tyr Asn Ile Tyr Pro
485 490 495
His Gly Ile Thr Asp Val Arg Pro Leu Tyr Ser Arg Arg Leu Pro Lys
500 505 510
Gly Val Lys His Leu Lys Asp Phe Pro Ile Leu Pro Gly Glu Ile Phe
515 520 525
Lys Tyr Lys Trp Thr Val Thr Val Glu Asp Gly Pro Thr Lys Ser Asp
530 535 540
Pro Arg Cys Leu Thr Arg Tyr Tyr Ser Ser Phe Val Asn Met Glu Arg
545 550 555 560
Asp Leu Ala Ser Gly Leu Ile Gly Pro Leu Leu Ile Cys Tyr Lys Glu
565 570 575
Ser Val Asp Gln Arg Gly Asn Gln Ile Met Ser Asp Lys Arg Asn Val
580 585 590
Ile Leu Phe Ser Val Phe Asp Glu Asn Arg Ser Trp Tyr Leu Thr Glu
595 600 605
Asn Ile Gln Arg Phe Leu Pro Asn Pro Ala Gly Val Gln Leu Glu Asp
610 615 620
Pro Glu Phe Gln Ala Ser Asn Ile Met His Ser Ile Asn Gly Tyr Val
625 630 635 640
Phe Asp Ser Leu Gln Leu Ser Val Cys Leu His Glu Val Ala Tyr Trp
645 650 655
Tyr Ile Leu Ser Ile Gly Ala Gln Thr Asp Phe Leu Ser Val Phe Phe
660 665 670
Ser Gly Tyr Thr Phe Lys His Lys Met Val Tyr Glu Asp Thr Leu Thr
675 680 685
Leu Phe Pro Phe Ser Gly Glu Thr Val Phe Met Ser Met Glu Asn Pro
690 695 700
Gly Leu Trp Ile Leu Ala Cys His Asn Ser Asp Phe Arg Asn Arg Gly
705 710 715 720
Met Thr Ala Leu Leu Lys Val Ser Ser Cys Asp Lys Asn Thr Gly Asp
725 730 735
Tyr Tyr Glu Asp Ser Tyr Glu Asp Ile Ser Ala Tyr Leu Leu Ser Lys
740 745 750
Asn Asn Ala Ile Glu Pro Arg Ser Phe Ser Gln Asn Ser Arg His Pro
755 760 765
Ser Gln Asn Pro Pro Val Leu Lys Arg His Gln Arg Glu Ile Thr Arg
770 775 780
Thr Thr Leu Gln Ser Asp Gln Glu Glu Ile Asp Tyr Asp Asp Thr Ile
785 790 795 800
Ser Val Glu Met Lys Lys Glu Asp Phe Asp Ile Tyr Asp Glu Asp Glu
805 810 815
Asn Gln Ser Pro Arg Ser Phe Gln Lys Lys Thr Arg His Tyr Phe Ile
820 825 830
Ala Ala Val Glu Arg Leu Trp Asp Tyr Gly Met Ser Ser Ser Pro His
835 840 845
Val Leu Arg Asn Arg Ala Gln Ser Gly Ser Val Pro Gln Phe Lys Lys
850 855 860
Val Val Phe Gln Glu Phe Thr Asp Gly Ser Phe Thr Gln Pro Leu Tyr
865 870 875 880
Arg Gly Glu Leu Asn Glu His Leu Gly Leu Leu Gly Pro Tyr Ile Arg
885 890 895
Ala Glu Val Glu Asp Asn Ile Met Val Thr Phe Arg Asn Gln Ala Ser
900 905 910
Arg Pro Tyr Ser Phe Tyr Ser Ser Leu Ile Ser Tyr Glu Glu Asp Gln
915 920 925
Arg Gln Gly Ala Glu Pro Arg Lys Asn Phe Val Lys Pro Asn Glu Thr
930 935 940
Lys Thr Tyr Phe Trp Lys Val Gln His His Met Ala Pro Thr Lys Asp
945 950 955 960
Glu Phe Asp Cys Lys Ala Trp Ala Tyr Phe Ser Asp Val Asp Leu Glu
965 970 975
Lys Asp Val His Ser Gly Leu Ile Gly Pro Leu Leu Val Cys His Thr
980 985 990
Asn Thr Leu Asn Pro Ala His Gly Arg Gln Val Thr Val Gln Glu Phe
995 1000 1005
Ala Leu Phe Phe Thr Ile Phe Asp Glu Thr Lys Ser Trp Tyr Phe Thr
1010 1015 1020
Glu Asn Met Glu Arg Asn Cys Arg Ala Pro Cys Asn Ile Gln Met Glu
1025 1030 1035 1040
Asp Pro Thr Phe Lys Glu Asn Tyr Arg Phe His Ala Ile Asn Gly Tyr
1045 1050 1055
Ile Met Asp Thr Leu Pro Gly Leu Val Met Ala Gln Asp Gln Arg Ile
1060 1065 1070
Arg Trp Tyr Leu Leu Ser Met Gly Ser Asn Glu Asn Ile His Ser Ile
1075 1080 1085
His Phe Ser Gly His Val Phe Thr Val Arg Lys Lys Glu Glu Tyr Lys
1090 1095 1100
Met Ala Leu Tyr Asn Leu Tyr Pro Gly Val Phe Glu Thr Val Glu Met
1105 1110 1115 1120
Leu Pro Ser Lys Ala Gly Ile Trp Arg Val Glu Cys Leu Ile Gly Glu
1125 1130 1135
His Leu His Ala Gly Met Ser Thr Leu Phe Leu Val Tyr Ser Asn Lys
1140 1145 1150
Cys Gln Thr Pro Leu Gly Met Ala Ser Gly His Ile Arg Asp Phe Gln
1155 1160 1165
Ile Thr Ala Ser Gly Gln Tyr Gly Gln Trp Ala Pro Lys Leu Ala Arg
1170 1175 1180
Leu His Tyr Ser Gly Ser Ile Asn Ala Trp Ser Thr Lys Glu Pro Phe
1185 1190 1195 1200
Ser Trp Ile Lys Val Asp Leu Leu Ala Pro Met Ile Ile His Gly Ile
1205 1210 1215
Lys Thr Gln Gly Ala Arg Gln Lys Phe Ser Ser Leu Tyr Ile Ser Gln
1220 1225 1230
Phe Ile Ile Met Tyr Ser Leu Asp Gly Lys Lys Trp Gln Thr Tyr Arg
1235 1240 1245
Gly Asn Ser Thr Gly Thr Leu Met Val Phe Phe Gly Asn Val Asp Ser
1250 1255 1260
Ser Gly Ile Lys His Asn Ile Phe Asn Pro Pro Ile Ile Ala Arg Tyr
1265 1270 1275 1280
Ile Arg Leu His Pro Thr His Tyr Ser Ile Arg Ser Thr Leu Arg Met
1285 1290 1295
Glu Leu Met Gly Cys Asp Leu Asn Ser Cys Ser Met Pro Leu Gly Met
1300 1305 1310
Glu Ser Lys Ala Ile Ser Asp Ala Gln Ile Thr Ala Ser Ser Tyr Phe
1315 1320 1325
Thr Asn Met Phe Ala Thr Trp Ser Pro Ser Lys Ala Arg Leu His Leu
1330 1335 1340
Gln Gly Arg Ser Asn Ala Trp Arg Pro Gln Val Asn Asn Pro Lys Glu
1345 1350 1355 1360
Trp Leu Gln Val Asp Phe Gln Lys Thr Met Lys Val Thr Gly Val Thr
1365 1370 1375
Thr Gln Gly Val Lys Ser Leu Leu Thr Ser Met Tyr Val Lys Glu Phe
1380 1385 1390
Leu Ile Ser Ser Ser Gln Asp Gly His Gln Trp Thr Leu Phe Phe Gln
1395 1400 1405
Asn Gly Lys Val Lys Val Phe Gln Gly Asn Gln Asp Ser Phe Thr Pro
1410 1415 1420
Val Val Asn Ser Leu Asp Pro Pro Leu Leu Thr Arg Tyr Leu Arg Ile
1425 1430 1435 1440
His Pro Gln Ser Trp Val His Gln Ile Ala Leu Arg Met Glu Val Leu
1445 1450 1455
Gly Cys Glu Ala Gln Asp Leu Tyr
1460
<210> 24
<211> 1463
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 24
Met Gln Ile Glu Leu Ser Thr Cys Phe Phe Leu Cys Leu Leu Arg Phe
1 5 10 15
Cys Phe Ser Ala Thr Arg Arg Tyr Tyr Leu Gly Ala Val Glu Leu Ser
20 25 30
Trp Asp Tyr Met Gln Ser Asp Leu Gly Glu Leu Pro Val Asp Ala Arg
35 40 45
Phe Pro Pro Arg Val Pro Lys Ser Phe Pro Phe Asn Thr Ser Val Val
50 55 60
Tyr Lys Lys Thr Leu Phe Val Glu Phe Thr Asp His Leu Phe Asn Ile
65 70 75 80
Ala Lys Pro Arg Pro Pro Trp Met Gly Leu Leu Gly Pro Thr Ile Gln
85 90 95
Ala Glu Val Tyr Asp Thr Val Val Ile Thr Leu Lys Asn Met Ala Ser
100 105 110
His Pro Val Ser Leu His Ala Val Gly Val Ser Tyr Trp Lys Ala Ser
115 120 125
Glu Gly Ala Glu Tyr Asp Asp Gln Thr Ser Gln Arg Glu Lys Glu Asp
130 135 140
Asp Lys Val Phe Pro Gly Gly Ser His Thr Tyr Val Trp Gln Val Leu
145 150 155 160
Lys Glu Asn Gly Pro Met Ala Ser Asp Pro Leu Cys Leu Thr Tyr Ser
165 170 175
Tyr Leu Ser His Val Asp Leu Val Lys Asp Leu Asn Ser Gly Leu Ile
180 185 190
Gly Ala Leu Leu Val Cys Arg Glu Gly Ser Leu Ala Lys Glu Lys Thr
195 200 205
Gln Thr Leu His Lys Phe Ile Leu Leu Phe Ala Val Phe Asp Glu Gly
210 215 220
Lys Ser Trp His Ser Glu Thr Lys Asn Ser Leu Met Gln Asp Arg Asp
225 230 235 240
Ala Ala Ser Ala Arg Ala Trp Pro Lys Met His Thr Val Asn Gly Tyr
245 250 255
Val Asn Arg Ser Leu Pro Gly Leu Ile Gly Cys His Arg Lys Ser Val
260 265 270
Tyr Trp His Val Ile Gly Met Gly Thr Thr Pro Glu Val His Ser Ile
275 280 285
Phe Leu Glu Gly His Thr Phe Leu Val Arg Asn His Arg Gln Ala Ser
290 295 300
Leu Glu Ile Ser Pro Ile Thr Phe Leu Thr Ala Gln Thr Leu Leu Met
305 310 315 320
Asp Leu Gly Gln Phe Leu Leu Phe Cys His Ile Ser Ser His Gln His
325 330 335
Asp Gly Met Glu Ala Tyr Val Lys Val Asp Ser Cys Pro Glu Glu Pro
340 345 350
Gln Leu Arg Met Lys Asn Asn Glu Glu Ala Glu Asp Tyr Asp Asp Asp
355 360 365
Leu Thr Asp Ser Glu Met Asp Val Val Arg Phe Asp Asp Asp Asn Ser
370 375 380
Pro Ser Phe Ile Gln Ile Arg Ser Val Ala Lys Lys His Pro Lys Thr
385 390 395 400
Trp Val His Tyr Ile Ala Ala Glu Glu Glu Asp Trp Asp Tyr Ala Pro
405 410 415
Leu Val Leu Ala Pro Asp Asp Arg Ser Tyr Lys Ser Gln Tyr Leu Asn
420 425 430
Asn Gly Pro Gln Arg Ile Gly Arg Lys Tyr Lys Lys Val Arg Phe Met
435 440 445
Ala Tyr Thr Asp Glu Thr Phe Lys Thr Arg Glu Ala Ile Gln His Glu
450 455 460
Ser Gly Ile Leu Gly Pro Leu Leu Tyr Gly Glu Val Gly Asp Thr Leu
465 470 475 480
Leu Ile Ile Phe Lys Asn Gln Ala Ser Arg Pro Tyr Asn Ile Tyr Pro
485 490 495
His Gly Ile Thr Asp Val Arg Pro Leu Tyr Ser Arg Arg Leu Pro Lys
500 505 510
Gly Val Lys His Leu Lys Asp Phe Pro Ile Leu Pro Gly Glu Ile Phe
515 520 525
Lys Tyr Lys Trp Thr Val Thr Val Glu Asp Gly Pro Thr Lys Ser Asp
530 535 540
Pro Arg Cys Leu Thr Arg Tyr Tyr Ser Ser Phe Val Asn Met Glu Arg
545 550 555 560
Asp Leu Ala Ser Gly Leu Ile Gly Pro Leu Leu Ile Cys Tyr Lys Glu
565 570 575
Ser Val Asp Gln Arg Gly Asn Gln Ile Met Ser Asp Lys Arg Asn Val
580 585 590
Ile Leu Phe Ser Val Phe Asp Glu Asn Arg Ser Trp Tyr Leu Thr Glu
595 600 605
Asn Ile Gln Arg Phe Leu Pro Asn Pro Ala Gly Val Gln Leu Glu Asp
610 615 620
Pro Glu Phe Gln Ala Ser Asn Ile Met His Ser Ile Asn Gly Tyr Val
625 630 635 640
Phe Asp Ser Leu Gln Leu Ser Val Cys Leu His Glu Val Ala Tyr Trp
645 650 655
Tyr Ile Leu Ser Ile Gly Ala Gln Thr Asp Phe Leu Ser Val Phe Phe
660 665 670
Ser Gly Tyr Thr Phe Lys His Lys Met Val Tyr Glu Asp Thr Leu Thr
675 680 685
Leu Phe Pro Phe Ser Gly Glu Thr Val Phe Met Ser Met Glu Asn Pro
690 695 700
Gly Leu Trp Ile Leu Ala Cys His Asn Ser Asp Phe Arg Asn Arg Gly
705 710 715 720
Met Thr Ala Leu Leu Lys Val Ser Ser Cys Asp Lys Asn Thr Gly Asp
725 730 735
Tyr Tyr Glu Asp Ser Tyr Glu Asp Ile Ser Ala Tyr Leu Leu Ser Lys
740 745 750
Asn Asn Ala Ile Glu Pro Arg Ser Phe Ser Gln Asn Ser Arg His Pro
755 760 765
Ser Thr Arg Gln Lys Gln Phe Asn Ala Thr Thr Ile Pro Glu Asn Thr
770 775 780
Thr Leu Gln Ser Asp Gln Glu Glu Ile Asp Tyr Asp Asp Thr Ile Ser
785 790 795 800
Val Glu Met Lys Lys Glu Asp Phe Asp Ile Tyr Asp Glu Asp Glu Asn
805 810 815
Gln Ser Pro Arg Ser Phe Gln Lys Lys Thr Arg His Tyr Phe Ile Ala
820 825 830
Ala Val Glu Arg Leu Trp Asp Tyr Gly Met Ser Ser Ser Pro His Val
835 840 845
Leu Arg Asn Arg Ala Gln Ser Gly Ser Val Pro Gln Phe Lys Lys Val
850 855 860
Val Phe Gln Glu Phe Thr Asp Gly Ser Phe Thr Gln Pro Leu Tyr Arg
865 870 875 880
Gly Glu Leu Asn Glu His Leu Gly Leu Leu Gly Pro Tyr Ile Arg Ala
885 890 895
Glu Val Glu Asp Asn Ile Met Val Thr Phe Arg Asn Gln Ala Ser Arg
900 905 910
Pro Tyr Ser Phe Tyr Ser Ser Leu Ile Ser Tyr Glu Glu Asp Gln Arg
915 920 925
Gln Gly Ala Glu Pro Arg Lys Asn Phe Val Lys Pro Asn Glu Thr Lys
930 935 940
Thr Tyr Phe Trp Lys Val Gln His His Met Ala Pro Thr Lys Asp Glu
945 950 955 960
Phe Asp Cys Lys Ala Trp Ala Tyr Phe Ser Asp Val Asp Leu Glu Lys
965 970 975
Asp Val His Ser Gly Leu Ile Gly Pro Leu Leu Val Cys His Thr Asn
980 985 990
Thr Leu Asn Pro Ala His Gly Arg Gln Val Thr Val Gln Glu Phe Ala
995 1000 1005
Leu Phe Phe Thr Ile Phe Asp Glu Thr Lys Ser Trp Tyr Phe Thr Glu
1010 1015 1020
Asn Met Glu Arg Asn Cys Arg Ala Pro Cys Asn Ile Gln Met Glu Asp
1025 1030 1035 1040
Pro Thr Phe Lys Glu Asn Tyr Arg Phe His Ala Ile Asn Gly Tyr Ile
1045 1050 1055
Met Asp Thr Leu Pro Gly Leu Val Met Ala Gln Asp Gln Arg Ile Arg
1060 1065 1070
Trp Tyr Leu Leu Ser Met Gly Ser Asn Glu Asn Ile His Ser Ile His
1075 1080 1085
Phe Ser Gly His Val Phe Thr Val Arg Lys Lys Glu Glu Tyr Lys Met
1090 1095 1100
Ala Leu Tyr Asn Leu Tyr Pro Gly Val Phe Glu Thr Val Glu Met Leu
1105 1110 1115 1120
Pro Ser Lys Ala Gly Ile Trp Arg Val Glu Cys Leu Ile Gly Glu His
1125 1130 1135
Leu His Ala Gly Met Ser Thr Leu Phe Leu Val Tyr Ser Asn Lys Cys
1140 1145 1150
Gln Thr Pro Leu Gly Met Ala Ser Gly His Ile Arg Asp Phe Gln Ile
1155 1160 1165
Thr Ala Ser Gly Gln Tyr Gly Gln Trp Ala Pro Lys Leu Ala Arg Leu
1170 1175 1180
His Tyr Ser Gly Ser Ile Asn Ala Trp Ser Thr Lys Glu Pro Phe Ser
1185 1190 1195 1200
Trp Ile Lys Val Asp Leu Leu Ala Pro Met Ile Ile His Gly Ile Lys
1205 1210 1215
Thr Gln Gly Ala Arg Gln Lys Phe Ser Ser Leu Tyr Ile Ser Gln Phe
1220 1225 1230
Ile Ile Met Tyr Ser Leu Asp Gly Lys Lys Trp Gln Thr Tyr Arg Gly
1235 1240 1245
Asn Ser Thr Gly Thr Leu Met Val Phe Phe Gly Asn Val Asp Ser Ser
1250 1255 1260
Gly Ile Lys His Asn Ile Phe Asn Pro Pro Ile Ile Ala Arg Tyr Ile
1265 1270 1275 1280
Arg Leu His Pro Thr His Tyr Ser Ile Arg Ser Thr Leu Arg Met Glu
1285 1290 1295
Leu Met Gly Cys Asp Leu Asn Ser Cys Ser Met Pro Leu Gly Met Glu
1300 1305 1310
Ser Lys Ala Ile Ser Asp Ala Gln Ile Thr Ala Ser Ser Tyr Phe Thr
1315 1320 1325
Asn Met Phe Ala Thr Trp Ser Pro Ser Lys Ala Arg Leu His Leu Gln
1330 1335 1340
Gly Arg Ser Asn Ala Trp Arg Pro Gln Val Asn Asn Pro Lys Glu Trp
1345 1350 1355 1360
Leu Gln Val Asp Phe Gln Lys Thr Met Lys Val Thr Gly Val Thr Thr
1365 1370 1375
Gln Gly Val Lys Ser Leu Leu Thr Ser Met Tyr Val Lys Glu Phe Leu
1380 1385 1390
Ile Ser Ser Ser Gln Asp Gly His Gln Trp Thr Leu Phe Phe Gln Asn
1395 1400 1405
Gly Lys Val Lys Val Phe Gln Gly Asn Gln Asp Ser Phe Thr Pro Val
1410 1415 1420
Val Asn Ser Leu Asp Pro Pro Leu Leu Thr Arg Tyr Leu Arg Ile His
1425 1430 1435 1440
Pro Gln Ser Trp Val His Gln Ile Ala Leu Arg Met Glu Val Leu Gly
1445 1450 1455
Cys Glu Ala Gln Asp Leu Tyr
1460
<210> 25
<211> 1453
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 25
Met Gln Ile Glu Leu Ser Thr Cys Phe Phe Leu Cys Leu Leu Arg Phe
1 5 10 15
Cys Phe Ser Ala Thr Arg Arg Tyr Tyr Leu Gly Ala Val Glu Leu Ser
20 25 30
Trp Asp Tyr Met Gln Ser Asp Leu Gly Glu Leu Pro Val Asp Ala Arg
35 40 45
Phe Pro Pro Arg Val Pro Lys Ser Phe Pro Phe Asn Thr Ser Val Val
50 55 60
Tyr Lys Lys Thr Leu Phe Val Glu Phe Thr Asp His Leu Phe Asn Ile
65 70 75 80
Ala Lys Pro Arg Pro Pro Trp Met Gly Leu Leu Gly Pro Thr Ile Gln
85 90 95
Ala Glu Val Tyr Asp Thr Val Val Ile Thr Leu Lys Asn Met Ala Ser
100 105 110
His Pro Val Ser Leu His Ala Val Gly Val Ser Tyr Trp Lys Ala Ser
115 120 125
Glu Gly Ala Glu Tyr Asp Asp Gln Thr Ser Gln Arg Glu Lys Glu Asp
130 135 140
Asp Lys Val Phe Pro Gly Gly Ser His Thr Tyr Val Trp Gln Val Leu
145 150 155 160
Lys Glu Asn Gly Pro Met Ala Ser Asp Pro Leu Cys Leu Thr Tyr Ser
165 170 175
Tyr Leu Ser His Val Asp Leu Val Lys Asp Leu Asn Ser Gly Leu Ile
180 185 190
Gly Ala Leu Leu Val Cys Arg Glu Gly Ser Leu Ala Lys Glu Lys Thr
195 200 205
Gln Thr Leu His Lys Phe Ile Leu Leu Phe Ala Val Phe Asp Glu Gly
210 215 220
Lys Ser Trp His Ser Glu Thr Lys Asn Ser Leu Met Gln Asp Arg Asp
225 230 235 240
Ala Ala Ser Ala Arg Ala Trp Pro Lys Met His Thr Val Asn Gly Tyr
245 250 255
Val Asn Arg Ser Leu Pro Gly Leu Ile Gly Cys His Arg Lys Ser Val
260 265 270
Tyr Trp His Val Ile Gly Met Gly Thr Thr Pro Glu Val His Ser Ile
275 280 285
Phe Leu Glu Gly His Thr Phe Leu Val Arg Asn His Arg Gln Ala Ser
290 295 300
Leu Glu Ile Ser Pro Ile Thr Phe Leu Thr Ala Gln Thr Leu Leu Met
305 310 315 320
Asp Leu Gly Gln Phe Leu Leu Phe Cys His Ile Ser Ser His Gln His
325 330 335
Asp Gly Met Glu Ala Tyr Val Lys Val Asp Ser Cys Pro Glu Glu Pro
340 345 350
Gln Leu Arg Met Lys Asn Asn Glu Glu Ala Glu Asp Tyr Asp Asp Asp
355 360 365
Leu Thr Asp Ser Glu Met Asp Val Val Arg Phe Asp Asp Asp Asn Ser
370 375 380
Pro Ser Phe Ile Gln Ile Arg Ser Val Ala Lys Lys His Pro Lys Thr
385 390 395 400
Trp Val His Tyr Ile Ala Ala Glu Glu Glu Asp Trp Asp Tyr Ala Pro
405 410 415
Leu Val Leu Ala Pro Asp Asp Arg Ser Tyr Lys Ser Gln Tyr Leu Asn
420 425 430
Asn Gly Pro Gln Arg Ile Gly Arg Lys Tyr Lys Lys Val Arg Phe Met
435 440 445
Ala Tyr Thr Asp Glu Thr Phe Lys Thr Arg Glu Ala Ile Gln His Glu
450 455 460
Ser Gly Ile Leu Gly Pro Leu Leu Tyr Gly Glu Val Gly Asp Thr Leu
465 470 475 480
Leu Ile Ile Phe Lys Asn Gln Ala Ser Arg Pro Tyr Asn Ile Tyr Pro
485 490 495
His Gly Ile Thr Asp Val Arg Pro Leu Tyr Ser Arg Arg Leu Pro Lys
500 505 510
Gly Val Lys His Leu Lys Asp Phe Pro Ile Leu Pro Gly Glu Ile Phe
515 520 525
Lys Tyr Lys Trp Thr Val Thr Val Glu Asp Gly Pro Thr Lys Ser Asp
530 535 540
Pro Arg Cys Leu Thr Arg Tyr Tyr Ser Ser Phe Val Asn Met Glu Arg
545 550 555 560
Asp Leu Ala Ser Gly Leu Ile Gly Pro Leu Leu Ile Cys Tyr Lys Glu
565 570 575
Ser Val Asp Gln Arg Gly Asn Gln Ile Met Ser Asp Lys Arg Asn Val
580 585 590
Ile Leu Phe Ser Val Phe Asp Glu Asn Arg Ser Trp Tyr Leu Thr Glu
595 600 605
Asn Ile Gln Arg Phe Leu Pro Asn Pro Ala Gly Val Gln Leu Glu Asp
610 615 620
Pro Glu Phe Gln Ala Ser Asn Ile Met His Ser Ile Asn Gly Tyr Val
625 630 635 640
Phe Asp Ser Leu Gln Leu Ser Val Cys Leu His Glu Val Ala Tyr Trp
645 650 655
Tyr Ile Leu Ser Ile Gly Ala Gln Thr Asp Phe Leu Ser Val Phe Phe
660 665 670
Ser Gly Tyr Thr Phe Lys His Lys Met Val Tyr Glu Asp Thr Leu Thr
675 680 685
Leu Phe Pro Phe Ser Gly Glu Thr Val Phe Met Ser Met Glu Asn Pro
690 695 700
Gly Leu Trp Ile Leu Ala Cys His Asn Ser Asp Phe Arg Asn Arg Gly
705 710 715 720
Met Thr Ala Leu Leu Lys Val Ser Ser Cys Asp Lys Asn Thr Gly Asp
725 730 735
Tyr Tyr Glu Asp Ser Tyr Glu Asp Ile Ser Ala Tyr Leu Leu Ser Lys
740 745 750
Asn Asn Ala Ile Glu Pro Arg Ser Phe Ser Gln Asn Pro Pro Val Leu
755 760 765
Lys Glu Ile Thr Arg Thr Thr Leu Gln Ser Asp Gln Glu Glu Ile Asp
770 775 780
Tyr Asp Asp Thr Ile Ser Val Glu Met Lys Lys Glu Asp Phe Asp Ile
785 790 795 800
Tyr Asp Glu Asp Glu Asn Gln Ser Pro Arg Ser Phe Gln Lys Lys Thr
805 810 815
Arg His Tyr Phe Ile Ala Ala Val Glu Arg Leu Trp Asp Tyr Gly Met
820 825 830
Ser Ser Ser Pro His Val Leu Arg Asn Arg Ala Gln Ser Gly Ser Val
835 840 845
Pro Gln Phe Lys Lys Val Val Phe Gln Glu Phe Thr Asp Gly Ser Phe
850 855 860
Thr Gln Pro Leu Tyr Arg Gly Glu Leu Asn Glu His Leu Gly Leu Leu
865 870 875 880
Gly Pro Tyr Ile Arg Ala Glu Val Glu Asp Asn Ile Met Val Thr Phe
885 890 895
Arg Asn Gln Ala Ser Arg Pro Tyr Ser Phe Tyr Ser Ser Leu Ile Ser
900 905 910
Tyr Glu Glu Asp Gln Arg Gln Gly Ala Glu Pro Arg Lys Asn Phe Val
915 920 925
Lys Pro Asn Glu Thr Lys Thr Tyr Phe Trp Lys Val Gln His His Met
930 935 940
Ala Pro Thr Lys Asp Glu Phe Asp Cys Lys Ala Trp Ala Tyr Phe Ser
945 950 955 960
Asp Val Asp Leu Glu Lys Asp Val His Ser Gly Leu Ile Gly Pro Leu
965 970 975
Leu Val Cys His Thr Asn Thr Leu Asn Pro Ala His Gly Arg Gln Val
980 985 990
Thr Val Gln Glu Phe Ala Leu Phe Phe Thr Ile Phe Asp Glu Thr Lys
995 1000 1005
Ser Trp Tyr Phe Thr Glu Asn Met Glu Arg Asn Cys Arg Ala Pro Cys
1010 1015 1020
Asn Ile Gln Met Glu Asp Pro Thr Phe Lys Glu Asn Tyr Arg Phe His
1025 1030 1035 1040
Ala Ile Asn Gly Tyr Ile Met Asp Thr Leu Pro Gly Leu Val Met Ala
1045 1050 1055
Gln Asp Gln Arg Ile Arg Trp Tyr Leu Leu Ser Met Gly Ser Asn Glu
1060 1065 1070
Asn Ile His Ser Ile His Phe Ser Gly His Val Phe Thr Val Arg Lys
1075 1080 1085
Lys Glu Glu Tyr Lys Met Ala Leu Tyr Asn Leu Tyr Pro Gly Val Phe
1090 1095 1100
Glu Thr Val Glu Met Leu Pro Ser Lys Ala Gly Ile Trp Arg Val Glu
1105 1110 1115 1120
Cys Leu Ile Gly Glu His Leu His Ala Gly Met Ser Thr Leu Phe Leu
1125 1130 1135
Val Tyr Ser Asn Lys Cys Gln Thr Pro Leu Gly Met Ala Ser Gly His
1140 1145 1150
Ile Arg Asp Phe Gln Ile Thr Ala Ser Gly Gln Tyr Gly Gln Trp Ala
1155 1160 1165
Pro Lys Leu Ala Arg Leu His Tyr Ser Gly Ser Ile Asn Ala Trp Ser
1170 1175 1180
Thr Lys Glu Pro Phe Ser Trp Ile Lys Val Asp Leu Leu Ala Pro Met
1185 1190 1195 1200
Ile Ile His Gly Ile Lys Thr Gln Gly Ala Arg Gln Lys Phe Ser Ser
1205 1210 1215
Leu Tyr Ile Ser Gln Phe Ile Ile Met Tyr Ser Leu Asp Gly Lys Lys
1220 1225 1230
Trp Gln Thr Tyr Arg Gly Asn Ser Thr Gly Thr Leu Met Val Phe Phe
1235 1240 1245
Gly Asn Val Asp Ser Ser Gly Ile Lys His Asn Ile Phe Asn Pro Pro
1250 1255 1260
Ile Ile Ala Arg Tyr Ile Arg Leu His Pro Thr His Tyr Ser Ile Arg
1265 1270 1275 1280
Ser Thr Leu Arg Met Glu Leu Met Gly Cys Asp Leu Asn Ser Cys Ser
1285 1290 1295
Met Pro Leu Gly Met Glu Ser Lys Ala Ile Ser Asp Ala Gln Ile Thr
1300 1305 1310
Ala Ser Ser Tyr Phe Thr Asn Met Phe Ala Thr Trp Ser Pro Ser Lys
1315 1320 1325
Ala Arg Leu His Leu Gln Gly Arg Ser Asn Ala Trp Arg Pro Gln Val
1330 1335 1340
Asn Asn Pro Lys Glu Trp Leu Gln Val Asp Phe Gln Lys Thr Met Lys
1345 1350 1355 1360
Val Thr Gly Val Thr Thr Gln Gly Val Lys Ser Leu Leu Thr Ser Met
1365 1370 1375
Tyr Val Lys Glu Phe Leu Ile Ser Ser Ser Gln Asp Gly His Gln Trp
1380 1385 1390
Thr Leu Phe Phe Gln Asn Gly Lys Val Lys Val Phe Gln Gly Asn Gln
1395 1400 1405
Asp Ser Phe Thr Pro Val Val Asn Ser Leu Asp Pro Pro Leu Leu Thr
1410 1415 1420
Arg Tyr Leu Arg Ile His Pro Gln Ser Trp Val His Gln Ile Ala Leu
1425 1430 1435 1440
Arg Met Glu Val Leu Gly Cys Glu Ala Gln Asp Leu Tyr
1445 1450
<210> 26
<211> 1474
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 26
Met Gln Ile Glu Leu Ser Thr Cys Phe Phe Leu Cys Leu Leu Arg Phe
1 5 10 15
Cys Phe Ser Ala Thr Arg Arg Tyr Tyr Leu Gly Ala Val Glu Leu Ser
20 25 30
Trp Asp Tyr Met Gln Ser Asp Leu Gly Glu Leu Pro Val Asp Ala Arg
35 40 45
Phe Pro Pro Arg Val Pro Lys Ser Phe Pro Phe Asn Thr Ser Val Val
50 55 60
Tyr Lys Lys Thr Leu Phe Val Glu Phe Thr Asp His Leu Phe Asn Ile
65 70 75 80
Ala Lys Pro Arg Pro Pro Trp Met Gly Leu Leu Gly Pro Thr Ile Gln
85 90 95
Ala Glu Val Tyr Asp Thr Val Val Ile Thr Leu Lys Asn Met Ala Ser
100 105 110
His Pro Val Ser Leu His Ala Val Gly Val Ser Tyr Trp Lys Ala Ser
115 120 125
Glu Gly Ala Glu Tyr Asp Asp Gln Thr Ser Gln Arg Glu Lys Glu Asp
130 135 140
Asp Lys Val Phe Pro Gly Gly Ser His Thr Tyr Val Trp Gln Val Leu
145 150 155 160
Lys Glu Asn Gly Pro Met Ala Ser Asp Pro Leu Cys Leu Thr Tyr Ser
165 170 175
Tyr Leu Ser His Val Asp Leu Val Lys Asp Leu Asn Ser Gly Leu Ile
180 185 190
Gly Ala Leu Leu Val Cys Arg Glu Gly Ser Leu Ala Lys Glu Lys Thr
195 200 205
Gln Thr Leu His Lys Phe Ile Leu Leu Phe Ala Val Phe Asp Glu Gly
210 215 220
Lys Ser Trp His Ser Glu Thr Lys Asn Ser Leu Met Gln Asp Arg Asp
225 230 235 240
Ala Ala Ser Ala Arg Ala Trp Pro Lys Met His Thr Val Asn Gly Tyr
245 250 255
Val Asn Arg Ser Leu Pro Gly Leu Ile Gly Cys His Arg Lys Ser Val
260 265 270
Tyr Trp His Val Ile Gly Met Gly Thr Thr Pro Glu Val His Ser Ile
275 280 285
Phe Leu Glu Gly His Thr Phe Leu Val Arg Asn His Arg Gln Ala Ser
290 295 300
Leu Glu Ile Ser Pro Ile Thr Phe Leu Thr Ala Gln Thr Leu Leu Met
305 310 315 320
Asp Leu Gly Gln Phe Leu Leu Phe Cys His Ile Ser Ser His Gln His
325 330 335
Asp Gly Met Glu Ala Tyr Val Lys Val Asp Ser Cys Pro Glu Glu Pro
340 345 350
Gln Leu Arg Met Lys Asn Asn Glu Glu Ala Glu Asp Tyr Asp Asp Asp
355 360 365
Leu Thr Asp Ser Glu Met Asp Val Val Arg Phe Asp Asp Asp Asn Ser
370 375 380
Pro Ser Phe Ile Gln Ile Arg Ser Val Ala Lys Lys His Pro Lys Thr
385 390 395 400
Trp Val His Tyr Ile Ala Ala Glu Glu Glu Asp Trp Asp Tyr Ala Pro
405 410 415
Leu Val Leu Ala Pro Asp Asp Arg Ser Tyr Lys Ser Gln Tyr Leu Asn
420 425 430
Asn Gly Pro Gln Arg Ile Gly Arg Lys Tyr Lys Lys Val Arg Phe Met
435 440 445
Ala Tyr Thr Asp Glu Thr Phe Lys Thr Arg Glu Ala Ile Gln His Glu
450 455 460
Ser Gly Ile Leu Gly Pro Leu Leu Tyr Gly Glu Val Gly Asp Thr Leu
465 470 475 480
Leu Ile Ile Phe Lys Asn Gln Ala Ser Arg Pro Tyr Asn Ile Tyr Pro
485 490 495
His Gly Ile Thr Asp Val Arg Pro Leu Tyr Ser Arg Arg Leu Pro Lys
500 505 510
Gly Val Lys His Leu Lys Asp Phe Pro Ile Leu Pro Gly Glu Ile Phe
515 520 525
Lys Tyr Lys Trp Thr Val Thr Val Glu Asp Gly Pro Thr Lys Ser Asp
530 535 540
Pro Arg Cys Leu Thr Arg Tyr Tyr Ser Ser Phe Val Asn Met Glu Arg
545 550 555 560
Asp Leu Ala Ser Gly Leu Ile Gly Pro Leu Leu Ile Cys Tyr Lys Glu
565 570 575
Ser Val Asp Gln Arg Gly Asn Gln Ile Met Ser Asp Lys Arg Asn Val
580 585 590
Ile Leu Phe Ser Val Phe Asp Glu Asn Arg Ser Trp Tyr Leu Thr Glu
595 600 605
Asn Ile Gln Arg Phe Leu Pro Asn Pro Ala Gly Val Gln Leu Glu Asp
610 615 620
Pro Glu Phe Gln Ala Ser Asn Ile Met His Ser Ile Asn Gly Tyr Val
625 630 635 640
Phe Asp Ser Leu Gln Leu Ser Val Cys Leu His Glu Val Ala Tyr Trp
645 650 655
Tyr Ile Leu Ser Ile Gly Ala Gln Thr Asp Phe Leu Ser Val Phe Phe
660 665 670
Ser Gly Tyr Thr Phe Lys His Lys Met Val Tyr Glu Asp Thr Leu Thr
675 680 685
Leu Phe Pro Phe Ser Gly Glu Thr Val Phe Met Ser Met Glu Asn Pro
690 695 700
Gly Leu Trp Ile Leu Ala Cys His Asn Ser Asp Phe Arg Asn Arg Gly
705 710 715 720
Met Thr Ala Leu Leu Lys Val Ser Ser Cys Asp Lys Asn Thr Gly Asp
725 730 735
Tyr Tyr Glu Asp Ser Tyr Glu Asp Ile Ser Ala Tyr Leu Leu Ser Lys
740 745 750
Asn Asn Ala Ile Glu Pro Arg Ser Phe Ser Gln Asn Ala Thr Asn Val
755 760 765
Ser Asn Asn Ser Asn Thr Ser Asn Asp Ser Asn Val Ser Pro Pro Val
770 775 780
Leu Lys Arg His Gln Arg Glu Ile Thr Arg Thr Thr Leu Gln Ser Asp
785 790 795 800
Gln Glu Glu Ile Asp Tyr Asp Asp Thr Ile Ser Val Glu Met Lys Lys
805 810 815
Glu Asp Phe Asp Ile Tyr Asp Glu Asp Glu Asn Gln Ser Pro Arg Ser
820 825 830
Phe Gln Lys Lys Thr Arg His Tyr Phe Ile Ala Ala Val Glu Arg Leu
835 840 845
Trp Asp Tyr Gly Met Ser Ser Ser Pro His Val Leu Arg Asn Arg Ala
850 855 860
Gln Ser Gly Ser Val Pro Gln Phe Lys Lys Val Val Phe Gln Glu Phe
865 870 875 880
Thr Asp Gly Ser Phe Thr Gln Pro Leu Tyr Arg Gly Glu Leu Asn Glu
885 890 895
His Leu Gly Leu Leu Gly Pro Tyr Ile Arg Ala Glu Val Glu Asp Asn
900 905 910
Ile Met Val Thr Phe Arg Asn Gln Ala Ser Arg Pro Tyr Ser Phe Tyr
915 920 925
Ser Ser Leu Ile Ser Tyr Glu Glu Asp Gln Arg Gln Gly Ala Glu Pro
930 935 940
Arg Lys Asn Phe Val Lys Pro Asn Glu Thr Lys Thr Tyr Phe Trp Lys
945 950 955 960
Val Gln His His Met Ala Pro Thr Lys Asp Glu Phe Asp Cys Lys Ala
965 970 975
Trp Ala Tyr Phe Ser Asp Val Asp Leu Glu Lys Asp Val His Ser Gly
980 985 990
Leu Ile Gly Pro Leu Leu Val Cys His Thr Asn Thr Leu Asn Pro Ala
995 1000 1005
His Gly Arg Gln Val Thr Val Gln Glu Phe Ala Leu Phe Phe Thr Ile
1010 1015 1020
Phe Asp Glu Thr Lys Ser Trp Tyr Phe Thr Glu Asn Met Glu Arg Asn
1025 1030 1035 1040
Cys Arg Ala Pro Cys Asn Ile Gln Met Glu Asp Pro Thr Phe Lys Glu
1045 1050 1055
Asn Tyr Arg Phe His Ala Ile Asn Gly Tyr Ile Met Asp Thr Leu Pro
1060 1065 1070
Gly Leu Val Met Ala Gln Asp Gln Arg Ile Arg Trp Tyr Leu Leu Ser
1075 1080 1085
Met Gly Ser Asn Glu Asn Ile His Ser Ile His Phe Ser Gly His Val
1090 1095 1100
Phe Thr Val Arg Lys Lys Glu Glu Tyr Lys Met Ala Leu Tyr Asn Leu
1105 1110 1115 1120
Tyr Pro Gly Val Phe Glu Thr Val Glu Met Leu Pro Ser Lys Ala Gly
1125 1130 1135
Ile Trp Arg Val Glu Cys Leu Ile Gly Glu His Leu His Ala Gly Met
1140 1145 1150
Ser Thr Leu Phe Leu Val Tyr Ser Asn Lys Cys Gln Thr Pro Leu Gly
1155 1160 1165
Met Ala Ser Gly His Ile Arg Asp Phe Gln Ile Thr Ala Ser Gly Gln
1170 1175 1180
Tyr Gly Gln Trp Ala Pro Lys Leu Ala Arg Leu His Tyr Ser Gly Ser
1185 1190 1195 1200
Ile Asn Ala Trp Ser Thr Lys Glu Pro Phe Ser Trp Ile Lys Val Asp
1205 1210 1215
Leu Leu Ala Pro Met Ile Ile His Gly Ile Lys Thr Gln Gly Ala Arg
1220 1225 1230
Gln Lys Phe Ser Ser Leu Tyr Ile Ser Gln Phe Ile Ile Met Tyr Ser
1235 1240 1245
Leu Asp Gly Lys Lys Trp Gln Thr Tyr Arg Gly Asn Ser Thr Gly Thr
1250 1255 1260
Leu Met Val Phe Phe Gly Asn Val Asp Ser Ser Gly Ile Lys His Asn
1265 1270 1275 1280
Ile Phe Asn Pro Pro Ile Ile Ala Arg Tyr Ile Arg Leu His Pro Thr
1285 1290 1295
His Tyr Ser Ile Arg Ser Thr Leu Arg Met Glu Leu Met Gly Cys Asp
1300 1305 1310
Leu Asn Ser Cys Ser Met Pro Leu Gly Met Glu Ser Lys Ala Ile Ser
1315 1320 1325
Asp Ala Gln Ile Thr Ala Ser Ser Tyr Phe Thr Asn Met Phe Ala Thr
1330 1335 1340
Trp Ser Pro Ser Lys Ala Arg Leu His Leu Gln Gly Arg Ser Asn Ala
1345 1350 1355 1360
Trp Arg Pro Gln Val Asn Asn Pro Lys Glu Trp Leu Gln Val Asp Phe
1365 1370 1375
Gln Lys Thr Met Lys Val Thr Gly Val Thr Thr Gln Gly Val Lys Ser
1380 1385 1390
Leu Leu Thr Ser Met Tyr Val Lys Glu Phe Leu Ile Ser Ser Ser Gln
1395 1400 1405
Asp Gly His Gln Trp Thr Leu Phe Phe Gln Asn Gly Lys Val Lys Val
1410 1415 1420
Phe Gln Gly Asn Gln Asp Ser Phe Thr Pro Val Val Asn Ser Leu Asp
1425 1430 1435 1440
Pro Pro Leu Leu Thr Arg Tyr Leu Arg Ile His Pro Gln Ser Trp Val
1445 1450 1455
His Gln Ile Ala Leu Arg Met Glu Val Leu Gly Cys Glu Ala Gln Asp
1460 1465 1470
Leu Tyr
<210> 27
<211> 1459
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 27
Met Gln Ile Glu Leu Ser Thr Cys Phe Phe Leu Cys Leu Leu Arg Phe
1 5 10 15
Cys Phe Ser Ala Thr Arg Arg Tyr Tyr Leu Gly Ala Val Glu Leu Ser
20 25 30
Trp Asp Tyr Met Gln Ser Asp Leu Gly Glu Leu Pro Val Asp Ala Arg
35 40 45
Phe Pro Pro Arg Val Pro Lys Ser Phe Pro Phe Asn Thr Ser Val Val
50 55 60
Tyr Lys Lys Thr Leu Phe Val Glu Phe Thr Asp His Leu Phe Asn Ile
65 70 75 80
Ala Lys Pro Arg Pro Pro Trp Met Gly Leu Leu Gly Pro Thr Ile Gln
85 90 95
Ala Glu Val Tyr Asp Thr Val Val Ile Thr Leu Lys Asn Met Ala Ser
100 105 110
His Pro Val Ser Leu His Ala Val Gly Val Ser Tyr Trp Lys Ala Ser
115 120 125
Glu Gly Ala Glu Tyr Asp Asp Gln Thr Ser Gln Arg Glu Lys Glu Asp
130 135 140
Asp Lys Val Phe Pro Gly Gly Ser His Thr Tyr Val Trp Gln Val Leu
145 150 155 160
Lys Glu Asn Gly Pro Met Ala Ser Asp Pro Leu Cys Leu Thr Tyr Ser
165 170 175
Tyr Leu Ser His Val Asp Leu Val Lys Asp Leu Asn Ser Gly Leu Ile
180 185 190
Gly Ala Leu Leu Val Cys Arg Glu Gly Ser Leu Ala Lys Glu Lys Thr
195 200 205
Gln Thr Leu His Lys Phe Ile Leu Leu Phe Ala Val Phe Asp Glu Gly
210 215 220
Lys Ser Trp His Ser Glu Thr Lys Asn Ser Leu Met Gln Asp Arg Asp
225 230 235 240
Ala Ala Ser Ala Arg Ala Trp Pro Lys Met His Thr Val Asn Gly Tyr
245 250 255
Val Asn Arg Ser Leu Pro Gly Leu Ile Gly Cys His Arg Lys Ser Val
260 265 270
Tyr Trp His Val Ile Gly Met Gly Thr Thr Pro Glu Val His Ser Ile
275 280 285
Phe Leu Glu Gly His Thr Phe Leu Val Arg Asn His Arg Gln Ala Ser
290 295 300
Leu Glu Ile Ser Pro Ile Thr Phe Leu Thr Ala Gln Thr Leu Leu Met
305 310 315 320
Asp Leu Gly Gln Phe Leu Leu Phe Cys His Ile Ser Ser His Gln His
325 330 335
Asp Gly Met Glu Ala Tyr Val Lys Val Asp Ser Cys Pro Glu Glu Pro
340 345 350
Gln Leu Arg Met Lys Asn Asn Glu Glu Ala Glu Asp Tyr Asp Asp Asp
355 360 365
Leu Thr Asp Ser Glu Met Asp Val Val Arg Phe Asp Asp Asp Asn Ser
370 375 380
Pro Ser Phe Ile Gln Ile Arg Ser Val Ala Lys Lys His Pro Lys Thr
385 390 395 400
Trp Val His Tyr Ile Ala Ala Glu Glu Glu Asp Trp Asp Tyr Ala Pro
405 410 415
Leu Val Leu Ala Pro Asp Asp Arg Ser Tyr Lys Ser Gln Tyr Leu Asn
420 425 430
Asn Gly Pro Gln Arg Ile Gly Arg Lys Tyr Lys Lys Val Arg Phe Met
435 440 445
Ala Tyr Thr Asp Glu Thr Phe Lys Thr Arg Glu Ala Ile Gln His Glu
450 455 460
Ser Gly Ile Leu Gly Pro Leu Leu Tyr Gly Glu Val Gly Asp Thr Leu
465 470 475 480
Leu Ile Ile Phe Lys Asn Gln Ala Ser Arg Pro Tyr Asn Ile Tyr Pro
485 490 495
His Gly Ile Thr Asp Val Arg Pro Leu Tyr Ser Arg Arg Leu Pro Lys
500 505 510
Gly Val Lys His Leu Lys Asp Phe Pro Ile Leu Pro Gly Glu Ile Phe
515 520 525
Lys Tyr Lys Trp Thr Val Thr Val Glu Asp Gly Pro Thr Lys Ser Asp
530 535 540
Pro Arg Cys Leu Thr Arg Tyr Tyr Ser Ser Phe Val Asn Met Glu Arg
545 550 555 560
Asp Leu Ala Ser Gly Leu Ile Gly Pro Leu Leu Ile Cys Tyr Lys Glu
565 570 575
Ser Val Asp Gln Arg Gly Asn Gln Ile Met Ser Asp Lys Arg Asn Val
580 585 590
Ile Leu Phe Ser Val Phe Asp Glu Asn Arg Ser Trp Tyr Leu Thr Glu
595 600 605
Asn Ile Gln Arg Phe Leu Pro Asn Pro Ala Gly Val Gln Leu Glu Asp
610 615 620
Pro Glu Phe Gln Ala Ser Asn Ile Met His Ser Ile Asn Gly Tyr Val
625 630 635 640
Phe Asp Ser Leu Gln Leu Ser Val Cys Leu His Glu Val Ala Tyr Trp
645 650 655
Tyr Ile Leu Ser Ile Gly Ala Gln Thr Asp Phe Leu Ser Val Phe Phe
660 665 670
Ser Gly Tyr Thr Phe Lys His Lys Met Val Tyr Glu Asp Thr Leu Thr
675 680 685
Leu Phe Pro Phe Ser Gly Glu Thr Val Phe Met Ser Met Glu Asn Pro
690 695 700
Gly Leu Trp Ile Leu Ala Cys His Asn Ser Asp Phe Arg Asn Arg Gly
705 710 715 720
Met Thr Ala Leu Leu Lys Val Ser Ser Cys Asp Lys Asn Thr Gly Asp
725 730 735
Tyr Tyr Glu Asp Ser Tyr Glu Asp Ile Ser Ala Tyr Leu Leu Ser Lys
740 745 750
Asn Asn Ala Ile Glu Pro Arg Ser Phe Ser Gln Asn Ser Arg His Gln
755 760 765
Ala Tyr Arg Tyr Arg Arg Gly Glu Ile Thr Arg Thr Thr Leu Gln Ser
770 775 780
Asp Gln Glu Glu Ile Asp Tyr Asp Asp Thr Ile Ser Val Glu Met Lys
785 790 795 800
Lys Glu Asp Phe Asp Ile Tyr Asp Glu Asp Glu Asn Gln Ser Pro Arg
805 810 815
Ser Phe Gln Lys Lys Thr Arg His Tyr Phe Ile Ala Ala Val Glu Arg
820 825 830
Leu Trp Asp Tyr Gly Met Ser Ser Ser Pro His Val Leu Arg Asn Arg
835 840 845
Ala Gln Ser Gly Ser Val Pro Gln Phe Lys Lys Val Val Phe Gln Glu
850 855 860
Phe Thr Asp Gly Ser Phe Thr Gln Pro Leu Tyr Arg Gly Glu Leu Asn
865 870 875 880
Glu His Leu Gly Leu Leu Gly Pro Tyr Ile Arg Ala Glu Val Glu Asp
885 890 895
Asn Ile Met Val Thr Phe Arg Asn Gln Ala Ser Arg Pro Tyr Ser Phe
900 905 910
Tyr Ser Ser Leu Ile Ser Tyr Glu Glu Asp Gln Arg Gln Gly Ala Glu
915 920 925
Pro Arg Lys Asn Phe Val Lys Pro Asn Glu Thr Lys Thr Tyr Phe Trp
930 935 940
Lys Val Gln His His Met Ala Pro Thr Lys Asp Glu Phe Asp Cys Lys
945 950 955 960
Ala Trp Ala Tyr Phe Ser Asp Val Asp Leu Glu Lys Asp Val His Ser
965 970 975
Gly Leu Ile Gly Pro Leu Leu Val Cys His Thr Asn Thr Leu Asn Pro
980 985 990
Ala His Gly Arg Gln Val Thr Val Gln Glu Phe Ala Leu Phe Phe Thr
995 1000 1005
Ile Phe Asp Glu Thr Lys Ser Trp Tyr Phe Thr Glu Asn Met Glu Arg
1010 1015 1020
Asn Cys Arg Ala Pro Cys Asn Ile Gln Met Glu Asp Pro Thr Phe Lys
1025 1030 1035 1040
Glu Asn Tyr Arg Phe His Ala Ile Asn Gly Tyr Ile Met Asp Thr Leu
1045 1050 1055
Pro Gly Leu Val Met Ala Gln Asp Gln Arg Ile Arg Trp Tyr Leu Leu
1060 1065 1070
Ser Met Gly Ser Asn Glu Asn Ile His Ser Ile His Phe Ser Gly His
1075 1080 1085
Val Phe Thr Val Arg Lys Lys Glu Glu Tyr Lys Met Ala Leu Tyr Asn
1090 1095 1100
Leu Tyr Pro Gly Val Phe Glu Thr Val Glu Met Leu Pro Ser Lys Ala
1105 1110 1115 1120
Gly Ile Trp Arg Val Glu Cys Leu Ile Gly Glu His Leu His Ala Gly
1125 1130 1135
Met Ser Thr Leu Phe Leu Val Tyr Ser Asn Lys Cys Gln Thr Pro Leu
1140 1145 1150
Gly Met Ala Ser Gly His Ile Arg Asp Phe Gln Ile Thr Ala Ser Gly
1155 1160 1165
Gln Tyr Gly Gln Trp Ala Pro Lys Leu Ala Arg Leu His Tyr Ser Gly
1170 1175 1180
Ser Ile Asn Ala Trp Ser Thr Lys Glu Pro Phe Ser Trp Ile Lys Val
1185 1190 1195 1200
Asp Leu Leu Ala Pro Met Ile Ile His Gly Ile Lys Thr Gln Gly Ala
1205 1210 1215
Arg Gln Lys Phe Ser Ser Leu Tyr Ile Ser Gln Phe Ile Ile Met Tyr
1220 1225 1230
Ser Leu Asp Gly Lys Lys Trp Gln Thr Tyr Arg Gly Asn Ser Thr Gly
1235 1240 1245
Thr Leu Met Val Phe Phe Gly Asn Val Asp Ser Ser Gly Ile Lys His
1250 1255 1260
Asn Ile Phe Asn Pro Pro Ile Ile Ala Arg Tyr Ile Arg Leu His Pro
1265 1270 1275 1280
Thr His Tyr Ser Ile Arg Ser Thr Leu Arg Met Glu Leu Met Gly Cys
1285 1290 1295
Asp Leu Asn Ser Cys Ser Met Pro Leu Gly Met Glu Ser Lys Ala Ile
1300 1305 1310
Ser Asp Ala Gln Ile Thr Ala Ser Ser Tyr Phe Thr Asn Met Phe Ala
1315 1320 1325
Thr Trp Ser Pro Ser Lys Ala Arg Leu His Leu Gln Gly Arg Ser Asn
1330 1335 1340
Ala Trp Arg Pro Gln Val Asn Asn Pro Lys Glu Trp Leu Gln Val Asp
1345 1350 1355 1360
Phe Gln Lys Thr Met Lys Val Thr Gly Val Thr Thr Gln Gly Val Lys
1365 1370 1375
Ser Leu Leu Thr Ser Met Tyr Val Lys Glu Phe Leu Ile Ser Ser Ser
1380 1385 1390
Gln Asp Gly His Gln Trp Thr Leu Phe Phe Gln Asn Gly Lys Val Lys
1395 1400 1405
Val Phe Gln Gly Asn Gln Asp Ser Phe Thr Pro Val Val Asn Ser Leu
1410 1415 1420
Asp Pro Pro Leu Leu Thr Arg Tyr Leu Arg Ile His Pro Gln Ser Trp
1425 1430 1435 1440
Val His Gln Ile Ala Leu Arg Met Glu Val Leu Gly Cys Glu Ala Gln
1445 1450 1455
Asp Leu Tyr
<210> 28
<211> 2133
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 28
Met Gln Leu Glu Leu Ser Thr Cys Val Phe Leu Cys Leu Leu Pro Leu
1 5 10 15
Gly Phe Ser Ala Ile Arg Arg Tyr Tyr Leu Gly Ala Val Glu Leu Ser
20 25 30
Trp Asp Tyr Arg Gln Ser Glu Leu Leu Arg Glu Leu His Val Asp Thr
35 40 45
Arg Phe Pro Ala Thr Ala Pro Gly Ala Leu Pro Leu Gly Pro Ser Val
50 55 60
Leu Tyr Lys Lys Thr Val Phe Val Glu Phe Thr Asp Gln Leu Phe Ser
65 70 75 80
Val Ala Arg Pro Arg Pro Pro Trp Met Gly Leu Leu Gly Pro Thr Ile
85 90 95
Gln Ala Glu Val Tyr Asp Thr Val Val Val Thr Leu Lys Asn Met Ala
100 105 110
Ser His Pro Val Ser Leu His Ala Val Gly Val Ser Phe Trp Lys Ser
115 120 125
Ser Glu Gly Ala Glu Tyr Glu Asp His Thr Ser Gln Arg Glu Lys Glu
130 135 140
Asp Asp Lys Val Leu Pro Gly Lys Ser Gln Thr Tyr Val Trp Gln Val
145 150 155 160
Leu Lys Glu Asn Gly Pro Thr Ala Ser Asp Pro Pro Cys Leu Thr Tyr
165 170 175
Ser Tyr Leu Ser His Val Asp Leu Val Lys Asp Leu Asn Ser Gly Leu
180 185 190
Ile Gly Ala Leu Leu Val Cys Arg Glu Gly Ser Leu Thr Arg Glu Arg
195 200 205
Thr Gln Asn Leu His Glu Phe Val Leu Leu Phe Ala Val Phe Asp Glu
210 215 220
Gly Lys Ser Trp His Ser Ala Arg Asn Asp Ser Trp Thr Arg Ala Met
225 230 235 240
Asp Pro Ala Pro Ala Arg Ala Gln Pro Ala Met His Thr Val Asn Gly
245 250 255
Tyr Val Asn Arg Ser Leu Pro Gly Leu Ile Gly Cys His Lys Lys Ser
260 265 270
Val Tyr Trp His Val Ile Gly Met Gly Thr Ser Pro Glu Val His Ser
275 280 285
Ile Phe Leu Glu Gly His Thr Phe Leu Val Arg His His Arg Gln Ala
290 295 300
Ser Leu Glu Ile Ser Pro Leu Thr Phe Leu Thr Ala Gln Thr Phe Leu
305 310 315 320
Met Asp Leu Gly Gln Phe Leu Leu Phe Cys His Ile Ser Ser His His
325 330 335
His Gly Gly Met Glu Ala His Val Arg Val Glu Ser Cys Ala Glu Glu
340 345 350
Pro Gln Leu Arg Arg Lys Ala Asp Glu Glu Glu Asp Tyr Asp Asp Asn
355 360 365
Leu Tyr Asp Ser Asp Met Asp Val Val Arg Leu Asp Gly Asp Asp Val
370 375 380
Ser Pro Phe Ile Gln Ile Arg Ser Val Ala Lys Lys His Pro Lys Thr
385 390 395 400
Trp Val His Tyr Ile Ser Ala Glu Glu Glu Asp Trp Asp Tyr Ala Pro
405 410 415
Ala Val Pro Ser Pro Ser Asp Arg Ser Tyr Lys Ser Leu Tyr Leu Asn
420 425 430
Ser Gly Pro Gln Arg Ile Gly Arg Lys Tyr Lys Lys Ala Arg Phe Val
435 440 445
Ala Tyr Thr Asp Val Thr Phe Lys Thr Arg Lys Ala Ile Pro Tyr Glu
450 455 460
Ser Gly Ile Leu Gly Pro Leu Leu Tyr Gly Glu Val Gly Asp Thr Leu
465 470 475 480
Leu Ile Ile Phe Lys Asn Lys Ala Ser Arg Pro Tyr Asn Ile Tyr Pro
485 490 495
His Gly Ile Thr Asp Val Ser Ala Leu His Pro Gly Arg Leu Leu Lys
500 505 510
Gly Trp Lys His Leu Lys Asp Met Pro Ile Leu Pro Gly Glu Thr Phe
515 520 525
Lys Tyr Lys Trp Thr Val Thr Val Glu Asp Gly Pro Thr Lys Ser Asp
530 535 540
Pro Arg Cys Leu Thr Arg Tyr Tyr Ser Ser Ser Ile Asn Leu Glu Lys
545 550 555 560
Asp Leu Ala Ser Gly Leu Ile Gly Pro Leu Leu Ile Cys Tyr Lys Glu
565 570 575
Ser Val Asp Gln Arg Gly Asn Gln Met Met Ser Asp Lys Arg Asn Val
580 585 590
Ile Leu Phe Ser Val Phe Asp Glu Asn Gln Ser Trp Tyr Leu Ala Glu
595 600 605
Asn Ile Gln Arg Phe Leu Pro Asn Pro Asp Gly Leu Gln Pro Gln Asp
610 615 620
Pro Glu Phe Gln Ala Ser Asn Ile Met His Ser Ile Asn Gly Tyr Val
625 630 635 640
Phe Asp Ser Leu Gln Leu Ser Val Cys Leu His Glu Val Ala Tyr Trp
645 650 655
Tyr Ile Leu Ser Val Gly Ala Gln Thr Asp Phe Leu Ser Val Phe Phe
660 665 670
Ser Gly Tyr Thr Phe Lys His Lys Met Val Tyr Glu Asp Thr Leu Thr
675 680 685
Leu Phe Pro Phe Ser Gly Glu Thr Val Phe Met Ser Met Glu Asn Pro
690 695 700
Gly Leu Trp Val Leu Ala Cys His Asn Ser Asp Leu Arg Asn Arg Gly
705 710 715 720
Met Thr Ala Leu Leu Lys Val Tyr Ser Cys Asp Arg Asp Ile Gly Asp
725 730 735
Tyr Tyr Asp Asn Thr Tyr Glu Asp Ile Pro Gly Phe Leu Leu Ser Gly
740 745 750
Lys Asn Val Ile Glu Pro Arg Ser Phe Ala Gln Asn Ser Arg Pro Pro
755 760 765
Ser Ala Ser Gln Lys Gln Phe Gln Thr Ile Thr Ser Pro Glu Asp Asp
770 775 780
Val Glu Leu Asp Pro Gln Ser Gly Glu Arg Thr Gln Ala Leu Glu Glu
785 790 795 800
Leu Ser Val Pro Ser Gly Asp Gly Ser Met Leu Leu Gly Gln Asn Pro
805 810 815
Ala Pro His Gly Ser Ser Ser Ser Asp Leu Gln Glu Ala Arg Asn Glu
820 825 830
Ala Asp Asp Tyr Leu Pro Gly Ala Arg Glu Arg Asn Thr Ala Pro Ser
835 840 845
Ala Ala Ala Arg Leu Arg Pro Glu Leu His His Ser Ala Glu Arg Val
850 855 860
Leu Thr Pro Glu Pro Glu Lys Glu Leu Lys Lys Leu Asp Ser Lys Met
865 870 875 880
Ser Ser Ser Ser Asp Leu Leu Lys Thr Ser Pro Thr Ile Pro Ser Asp
885 890 895
Thr Leu Ser Ala Glu Thr Glu Arg Thr His Ser Leu Gly Pro Pro His
900 905 910
Pro Gln Val Asn Phe Arg Ser Gln Leu Gly Ala Ile Val Leu Gly Lys
915 920 925
Asn Ser Ser His Phe Ile Gly Ala Gly Val Pro Leu Gly Ser Thr Glu
930 935 940
Glu Asp His Glu Ser Ser Leu Gly Glu Asn Val Ser Pro Val Glu Ser
945 950 955 960
Asp Gly Ile Phe Glu Lys Glu Arg Ala His Gly Pro Ala Ser Leu Thr
965 970 975
Lys Asp Asp Val Leu Phe Lys Val Asn Ile Ser Leu Val Lys Thr Asn
980 985 990
Lys Ala Arg Val Tyr Leu Lys Thr Asn Arg Lys Ile His Ile Asp Asp
995 1000 1005
Ala Ala Leu Leu Thr Glu Asn Arg Ala Ser Ala Thr Phe Met Asp Lys
1010 1015 1020
Asn Thr Thr Ala Ser Gly Leu Asn His Val Ser Asn Trp Ile Lys Gly
1025 1030 1035 1040
Pro Leu Gly Lys Asn Pro Leu Ser Ser Glu Arg Gly Pro Ser Pro Glu
1045 1050 1055
Leu Leu Thr Ser Ser Gly Ser Gly Lys Ser Val Lys Gly Gln Ser Ser
1060 1065 1070
Gly Gln Gly Arg Ile Arg Val Ala Val Glu Glu Glu Glu Leu Ser Lys
1075 1080 1085
Gly Lys Glu Met Met Leu Pro Asn Ser Glu Leu Thr Phe Leu Thr Asn
1090 1095 1100
Ser Ala Asp Val Gln Gly Asn Asp Thr His Ser Gln Gly Lys Lys Ser
1105 1110 1115 1120
Arg Glu Glu Met Glu Arg Arg Glu Lys Leu Val Gln Glu Lys Val Asp
1125 1130 1135
Leu Pro Gln Val Tyr Thr Ala Thr Gly Thr Lys Asn Phe Leu Arg Asn
1140 1145 1150
Ile Phe His Gln Ser Thr Glu Pro Ser Val Glu Gly Phe Asp Gly Gly
1155 1160 1165
Ser His Ala Pro Val Pro Gln Asp Ser Arg Ser Leu Asn Asp Ser Ala
1170 1175 1180
Glu Arg Ala Glu Thr His Ile Ala His Phe Ser Ala Ile Arg Glu Glu
1185 1190 1195 1200
Ala Pro Leu Glu Ala Pro Gly Asn Arg Thr Gly Pro Gly Pro Arg Ser
1205 1210 1215
Ala Val Pro Arg Arg Val Lys Gln Ser Leu Lys Gln Ile Arg Leu Pro
1220 1225 1230
Leu Glu Glu Ile Lys Pro Glu Arg Gly Val Val Leu Asn Ala Thr Ser
1235 1240 1245
Thr Arg Trp Ser Glu Ser Ser Pro Ile Leu Gln Gly Ala Lys Arg Asn
1250 1255 1260
Asn Leu Ser Leu Pro Phe Leu Thr Leu Glu Met Ala Gly Gly Gln Gly
1265 1270 1275 1280
Lys Ile Ser Ala Leu Gly Lys Ser Ala Ala Gly Pro Leu Ala Ser Gly
1285 1290 1295
Lys Leu Glu Lys Ala Val Leu Ser Ser Ala Gly Leu Ser Glu Ala Ser
1300 1305 1310
Gly Lys Ala Glu Phe Leu Pro Lys Val Arg Val His Arg Glu Asp Leu
1315 1320 1325
Leu Pro Gln Lys Thr Ser Asn Val Ser Cys Ala His Gly Asp Leu Gly
1330 1335 1340
Gln Glu Ile Phe Leu Gln Lys Thr Arg Gly Pro Val Asn Leu Asn Lys
1345 1350 1355 1360
Val Asn Arg Pro Gly Arg Thr Pro Ser Lys Leu Leu Gly Pro Pro Met
1365 1370 1375
Pro Lys Glu Trp Glu Ser Leu Glu Lys Ser Pro Lys Ser Thr Ala Leu
1380 1385 1390
Arg Thr Lys Asp Ile Ile Ser Leu Pro Leu Asp Arg His Glu Ser Asn
1395 1400 1405
His Ser Ile Ala Ala Lys Asn Glu Gly Gln Ala Glu Thr Gln Arg Glu
1410 1415 1420
Ala Ala Trp Thr Lys Gln Gly Gly Pro Gly Arg Leu Cys Ala Pro Lys
1425 1430 1435 1440
Pro Pro Val Leu Arg Arg His Gln Arg Asp Ile Ser Leu Pro Thr Phe
1445 1450 1455
Gln Pro Glu Glu Asp Lys Met Asp Tyr Asp Asp Ile Phe Ser Thr Glu
1460 1465 1470
Thr Lys Gly Glu Asp Phe Asp Ile Tyr Gly Glu Asp Glu Asn Gln Asp
1475 1480 1485
Pro Arg Ser Phe Gln Lys Arg Thr Arg His Tyr Phe Ile Ala Ala Val
1490 1495 1500
Glu Gln Leu Trp Asp Tyr Gly Met Ser Glu Ser Pro Arg Ala Leu Arg
1505 1510 1515 1520
Asn Arg Ala Gln Asn Gly Glu Val Pro Arg Phe Lys Lys Val Val Phe
1525 1530 1535
Arg Glu Phe Ala Asp Gly Ser Phe Thr Gln Pro Ser Tyr Arg Gly Glu
1540 1545 1550
Leu Asn Lys His Leu Gly Leu Leu Gly Pro Tyr Ile Arg Ala Glu Val
1555 1560 1565
Glu Asp Asn Ile Met Val Thr Phe Lys Asn Gln Ala Ser Arg Pro Tyr
1570 1575 1580
Ser Phe Tyr Ser Ser Leu Ile Ser Tyr Pro Asp Asp Gln Glu Gln Gly
1585 1590 1595 1600
Ala Glu Pro Arg His Asn Phe Val Gln Pro Asn Glu Thr Arg Thr Tyr
1605 1610 1615
Phe Trp Lys Val Gln His His Met Ala Pro Thr Glu Asp Glu Phe Asp
1620 1625 1630
Cys Lys Ala Trp Ala Tyr Phe Ser Asp Val Asp Leu Glu Lys Asp Val
1635 1640 1645
His Ser Gly Leu Ile Gly Pro Leu Leu Ile Cys Arg Ala Asn Thr Leu
1650 1655 1660
Asn Ala Ala His Gly Arg Gln Val Thr Val Gln Glu Phe Ala Leu Phe
1665 1670 1675 1680
Phe Thr Ile Phe Asp Glu Thr Lys Ser Trp Tyr Phe Thr Glu Asn Val
1685 1690 1695
Glu Arg Asn Cys Arg Ala Pro Cys His Leu Gln Met Glu Asp Pro Thr
1700 1705 1710
Leu Lys Glu Asn Tyr Arg Phe His Ala Ile Asn Gly Tyr Val Met Asp
1715 1720 1725
Thr Leu Pro Gly Leu Val Met Ala Gln Asn Gln Arg Ile Arg Trp Tyr
1730 1735 1740
Leu Leu Ser Met Gly Ser Asn Glu Asn Ile His Ser Ile His Phe Ser
1745 1750 1755 1760
Gly His Val Phe Ser Val Arg Lys Lys Glu Glu Tyr Lys Met Ala Val
1765 1770 1775
Tyr Asn Leu Tyr Pro Gly Val Phe Glu Thr Val Glu Met Leu Pro Ser
1780 1785 1790
Lys Val Gly Ile Trp Arg Ile Glu Cys Leu Ile Gly Glu His Leu Gln
1795 1800 1805
Ala Gly Met Ser Thr Thr Phe Leu Val Tyr Ser Lys Glu Cys Gln Ala
1810 1815 1820
Pro Leu Gly Met Ala Ser Gly Arg Ile Arg Asp Phe Gln Ile Thr Ala
1825 1830 1835 1840
Ser Gly Gln Tyr Gly Gln Trp Ala Pro Lys Leu Ala Arg Leu His Tyr
1845 1850 1855
Ser Gly Ser Ile Asn Ala Trp Ser Thr Lys Asp Pro His Ser Trp Ile
1860 1865 1870
Lys Val Asp Leu Leu Ala Pro Met Ile Ile His Gly Ile Met Thr Gln
1875 1880 1885
Gly Ala Arg Gln Lys Phe Ser Ser Leu Tyr Ile Ser Gln Phe Ile Ile
1890 1895 1900
Met Tyr Ser Leu Asp Gly Arg Asn Trp Gln Ser Tyr Arg Gly Asn Ser
1905 1910 1915 1920
Thr Gly Thr Leu Met Val Phe Phe Gly Asn Val Asp Ala Ser Gly Ile
1925 1930 1935
Lys His Asn Ile Phe Asn Pro Pro Ile Val Ala Arg Tyr Ile Arg Leu
1940 1945 1950
His Pro Thr His Tyr Ser Ile Arg Ser Thr Leu Arg Met Glu Leu Met
1955 1960 1965
Gly Cys Asp Leu Asn Ser Cys Ser Met Pro Leu Gly Met Gln Asn Lys
1970 1975 1980
Ala Ile Ser Asp Ser Gln Ile Thr Ala Ser Ser His Leu Ser Asn Ile
1985 1990 1995 2000
Phe Ala Thr Trp Ser Pro Ser Gln Ala Arg Leu His Leu Gln Gly Arg
2005 2010 2015
Thr Asn Ala Trp Arg Pro Arg Val Ser Ser Ala Glu Glu Trp Leu Gln
2020 2025 2030
Val Asp Leu Gln Lys Thr Val Lys Val Thr Gly Ile Thr Thr Gln Gly
2035 2040 2045
Val Lys Ser Leu Leu Ser Ser Met Tyr Val Lys Glu Phe Leu Val Ser
2050 2055 2060
Ser Ser Gln Asp Gly Arg Arg Trp Thr Leu Phe Leu Gln Asp Gly His
2065 2070 2075 2080
Thr Lys Val Phe Gln Gly Asn Gln Asp Ser Ser Thr Pro Val Val Asn
2085 2090 2095
Ala Leu Asp Pro Pro Leu Phe Thr Arg Tyr Leu Arg Ile His Pro Thr
2100 2105 2110
Ser Trp Ala Gln His Ile Ala Leu Arg Leu Glu Val Leu Gly Cys Glu
2115 2120 2125
Ala Gln Asp Leu Tyr
2130
<210> 29
<211> 1466
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 29
Met Gln Leu Glu Leu Ser Thr Cys Val Phe Leu Cys Leu Leu Pro Leu
1 5 10 15
Gly Phe Ser Ala Ile Arg Arg Tyr Tyr Leu Gly Ala Val Glu Leu Ser
20 25 30
Trp Asp Tyr Arg Gln Ser Glu Leu Leu Arg Glu Leu His Val Asp Thr
35 40 45
Arg Phe Pro Ala Thr Ala Pro Gly Ala Leu Pro Leu Gly Pro Ser Val
50 55 60
Leu Tyr Lys Lys Thr Val Phe Val Glu Phe Thr Asp Gln Leu Phe Ser
65 70 75 80
Val Ala Arg Pro Arg Pro Pro Trp Met Gly Leu Leu Gly Pro Thr Ile
85 90 95
Gln Ala Glu Val Tyr Asp Thr Val Val Val Thr Leu Lys Asn Met Ala
100 105 110
Ser His Pro Val Ser Leu His Ala Val Gly Val Ser Phe Trp Lys Ser
115 120 125
Ser Glu Gly Ala Glu Tyr Glu Asp His Thr Ser Gln Arg Glu Lys Glu
130 135 140
Asp Asp Lys Val Leu Pro Gly Lys Ser Gln Thr Tyr Val Trp Gln Val
145 150 155 160
Leu Lys Glu Asn Gly Pro Thr Ala Ser Asp Pro Pro Cys Leu Thr Tyr
165 170 175
Ser Tyr Leu Ser His Val Asp Leu Val Lys Asp Leu Asn Ser Gly Leu
180 185 190
Ile Gly Ala Leu Leu Val Cys Arg Glu Gly Ser Leu Thr Arg Glu Arg
195 200 205
Thr Gln Asn Leu His Glu Phe Val Leu Leu Phe Ala Val Phe Asp Glu
210 215 220
Gly Lys Ser Trp His Ser Ala Arg Asn Asp Ser Trp Thr Arg Ala Met
225 230 235 240
Asp Pro Ala Pro Ala Arg Ala Gln Pro Ala Met His Thr Val Asn Gly
245 250 255
Tyr Val Asn Arg Ser Leu Pro Gly Leu Ile Gly Cys His Lys Lys Ser
260 265 270
Val Tyr Trp His Val Ile Gly Met Gly Thr Ser Pro Glu Val His Ser
275 280 285
Ile Phe Leu Glu Gly His Thr Phe Leu Val Arg His His Arg Gln Ala
290 295 300
Ser Leu Glu Ile Ser Pro Leu Thr Phe Leu Thr Ala Gln Thr Phe Leu
305 310 315 320
Met Asp Leu Gly Gln Phe Leu Leu Phe Cys His Ile Ser Ser His His
325 330 335
His Gly Gly Met Glu Ala His Val Arg Val Glu Ser Cys Ala Glu Glu
340 345 350
Pro Gln Leu Arg Arg Lys Ala Asp Glu Glu Glu Asp Tyr Asp Asp Asn
355 360 365
Leu Tyr Asp Ser Asp Met Asp Val Val Arg Leu Asp Gly Asp Asp Val
370 375 380
Ser Pro Phe Ile Gln Ile Arg Ser Val Ala Lys Lys His Pro Lys Thr
385 390 395 400
Trp Val His Tyr Ile Ser Ala Glu Glu Glu Asp Trp Asp Tyr Ala Pro
405 410 415
Ala Val Pro Ser Pro Ser Asp Arg Ser Tyr Lys Ser Leu Tyr Leu Asn
420 425 430
Ser Gly Pro Gln Arg Ile Gly Arg Lys Tyr Lys Lys Ala Arg Phe Val
435 440 445
Ala Tyr Thr Asp Val Thr Phe Lys Thr Arg Lys Ala Ile Pro Tyr Glu
450 455 460
Ser Gly Ile Leu Gly Pro Leu Leu Tyr Gly Glu Val Gly Asp Thr Leu
465 470 475 480
Leu Ile Ile Phe Lys Asn Lys Ala Ser Arg Pro Tyr Asn Ile Tyr Pro
485 490 495
His Gly Ile Thr Asp Val Ser Ala Leu His Pro Gly Arg Leu Leu Lys
500 505 510
Gly Trp Lys His Leu Lys Asp Met Pro Ile Leu Pro Gly Glu Thr Phe
515 520 525
Lys Tyr Lys Trp Thr Val Thr Val Glu Asp Gly Pro Thr Lys Ser Asp
530 535 540
Pro Arg Cys Leu Thr Arg Tyr Tyr Ser Ser Ser Ile Asn Leu Glu Lys
545 550 555 560
Asp Leu Ala Ser Gly Leu Ile Gly Pro Leu Leu Ile Cys Tyr Lys Glu
565 570 575
Ser Val Asp Gln Arg Gly Asn Gln Met Met Ser Asp Lys Arg Asn Val
580 585 590
Ile Leu Phe Ser Val Phe Asp Glu Asn Gln Ser Trp Tyr Leu Ala Glu
595 600 605
Asn Ile Gln Arg Phe Leu Pro Asn Pro Asp Gly Leu Gln Pro Gln Asp
610 615 620
Pro Glu Phe Gln Ala Ser Asn Ile Met His Ser Ile Asn Gly Tyr Val
625 630 635 640
Phe Asp Ser Leu Gln Leu Ser Val Cys Leu His Glu Val Ala Tyr Trp
645 650 655
Tyr Ile Leu Ser Val Gly Ala Gln Thr Asp Phe Leu Ser Val Phe Phe
660 665 670
Ser Gly Tyr Thr Phe Lys His Lys Met Val Tyr Glu Asp Thr Leu Thr
675 680 685
Leu Phe Pro Phe Ser Gly Glu Thr Val Phe Met Ser Met Glu Asn Pro
690 695 700
Gly Leu Trp Val Leu Ala Cys His Asn Ser Asp Leu Arg Asn Arg Gly
705 710 715 720
Met Thr Ala Leu Leu Lys Val Tyr Ser Cys Asp Arg Asp Ile Gly Asp
725 730 735
Tyr Tyr Asp Asn Thr Tyr Glu Asp Ile Pro Gly Phe Leu Leu Ser Gly
740 745 750
Lys Asn Val Ile Glu Pro Arg Ser Phe Ser Gln Asn Ser Arg Pro Pro
755 760 765
Ser Ala Ser Ala Pro Lys Pro Pro Val Leu Arg His Gln Arg Asp Ile
770 775 780
Ser Leu Pro Thr Arg Gln Pro Glu Glu Asp Lys Met Asp Tyr Asp Asp
785 790 795 800
Ile Phe Ser Thr Glu Thr Lys Gly Glu Asp Phe Asp Ile Tyr Gly Glu
805 810 815
Asp Glu Asn Gln Asp Pro Arg Ser Phe Gln Lys Arg Thr Arg His Tyr
820 825 830
Phe Ile Ala Ala Val Glu Gln Leu Trp Asp Tyr Gly Met Ser Glu Ser
835 840 845
Pro Arg Ala Leu Arg Asn Arg Ala Gln Asn Gly Glu Val Pro Arg Phe
850 855 860
Lys Lys Val Val Phe Arg Glu Phe Ala Asp Gly Ser Phe Thr Gln Pro
865 870 875 880
Ser Tyr Arg Gly Glu Leu Asn Lys His Leu Gly Leu Leu Gly Pro Tyr
885 890 895
Ile Arg Ala Glu Val Glu Asp Asn Ile Met Val Thr Phe Lys Asn Gln
900 905 910
Ala Ser Arg Pro Tyr Ser Phe Tyr Ser Ser Leu Ile Ser Tyr Pro Asp
915 920 925
Asp Gln Glu Gln Gly Ala Glu Pro Arg His Asn Phe Val Gln Pro Asn
930 935 940
Glu Thr Arg Thr Tyr Phe Trp Lys Val Gln His His Met Ala Pro Thr
945 950 955 960
Glu Asp Glu Phe Asp Cys Lys Ala Trp Ala Tyr Phe Ser Asp Val Asp
965 970 975
Leu Glu Lys Asp Val His Ser Gly Leu Ile Gly Pro Leu Leu Ile Cys
980 985 990
Arg Ala Asn Thr Leu Asn Ala Ala His Gly Arg Gln Val Thr Val Gln
995 1000 1005
Glu Phe Ala Leu Phe Phe Thr Ile Phe Asp Glu Thr Lys Ser Trp Tyr
1010 1015 1020
Phe Thr Glu Asn Val Glu Arg Asn Cys Arg Ala Pro Cys His Leu Gln
1025 1030 1035 1040
Met Glu Asp Pro Thr Leu Lys Glu Asn Tyr Arg Phe His Ala Ile Asn
1045 1050 1055
Gly Tyr Val Met Asp Thr Leu Pro Gly Leu Val Met Ala Gln Asn Gln
1060 1065 1070
Arg Ile Arg Trp Tyr Leu Leu Ser Met Gly Ser Asn Glu Asn Ile His
1075 1080 1085
Ser Ile His Phe Ser Gly His Val Phe Ser Val Arg Lys Lys Glu Glu
1090 1095 1100
Tyr Lys Met Ala Val Tyr Asn Leu Tyr Pro Gly Val Phe Glu Thr Val
1105 1110 1115 1120
Glu Met Leu Pro Ser Lys Val Gly Ile Trp Arg Ile Glu Cys Leu Ile
1125 1130 1135
Gly Glu His Leu Gln Ala Gly Met Ser Thr Thr Phe Leu Val Tyr Ser
1140 1145 1150
Lys Glu Cys Gln Ala Pro Leu Gly Met Ala Ser Gly Arg Ile Arg Asp
1155 1160 1165
Phe Gln Ile Thr Ala Ser Gly Gln Tyr Gly Gln Trp Ala Pro Lys Leu
1170 1175 1180
Ala Arg Leu His Tyr Ser Gly Ser Ile Asn Ala Trp Ser Thr Lys Asp
1185 1190 1195 1200
Pro His Ser Trp Ile Lys Val Asp Leu Leu Ala Pro Met Ile Ile His
1205 1210 1215
Gly Ile Met Thr Gln Gly Ala Arg Gln Lys Phe Ser Ser Leu Tyr Ile
1220 1225 1230
Ser Gln Phe Ile Ile Met Tyr Ser Leu Asp Gly Arg Asn Trp Gln Ser
1235 1240 1245
Tyr Arg Gly Asn Ser Thr Gly Thr Leu Met Val Phe Phe Gly Asn Val
1250 1255 1260
Asp Ala Ser Gly Ile Lys His Asn Ile Phe Asn Pro Pro Ile Val Ala
1265 1270 1275 1280
Arg Tyr Ile Arg Leu His Pro Thr His Tyr Ser Ile Arg Ser Thr Leu
1285 1290 1295
Arg Met Glu Leu Met Gly Cys Asp Leu Asn Ser Cys Ser Met Pro Leu
1300 1305 1310
Gly Met Gln Asn Lys Ala Ile Ser Asp Ser Gln Ile Thr Ala Ser Ser
1315 1320 1325
His Leu Ser Asn Ile Phe Ala Thr Trp Ser Pro Ser Gln Ala Arg Leu
1330 1335 1340
His Leu Gln Gly Arg Thr Asn Ala Trp Arg Pro Arg Val Ser Ser Ala
1345 1350 1355 1360
Glu Glu Trp Leu Gln Val Asp Leu Gln Lys Thr Val Lys Val Thr Gly
1365 1370 1375
Ile Thr Thr Gln Gly Val Lys Ser Leu Leu Ser Ser Met Tyr Val Lys
1380 1385 1390
Glu Phe Leu Val Ser Ser Ser Gln Asp Gly Arg Arg Trp Thr Leu Phe
1395 1400 1405
Leu Gln Asp Gly His Thr Lys Val Phe Gln Gly Asn Gln Asp Ser Ser
1410 1415 1420
Thr Pro Val Val Asn Ala Leu Asp Pro Pro Leu Phe Thr Arg Tyr Leu
1425 1430 1435 1440
Arg Ile His Pro Thr Ser Trp Ala Gln His Ile Ala Leu Arg Leu Glu
1445 1450 1455
Val Leu Gly Cys Glu Ala Gln Asp Leu Tyr
1460 1465
Claims (9)
1. A nucleic acid encoding a variant Gly710Ala of a highly active factor viii or villa polypeptide, wherein:
(1) hF VIII cDNA: the nucleotide sequence is shown in any one of SEQ ID NO 1-4;
or (2) BDD hF VIII-SQ: the nucleotide sequence is shown as any one of SEQ ID NO 5-8;
or (3) BDD hFVIII-N8: the nucleotide sequence is shown in any one of SEQ ID NO 9-12;
or (4) BDD hFVIII-SC: the nucleotide sequence is shown in any one of SEQ ID NO 13-16;
or (5) BDD hF VIII- Δ F: the nucleotide sequence is shown in any one of SEQ ID NO 17-20.
2. A mutein of a highly active factor VIII or VIIIa polypeptide variant Gly710Ala characterized in that: the amino acid sequence is shown in any one of SEQ ID NO 21-29, and the 710 th amino acid of the mutant is Ala but not Gly of wild type VIII or VIII a.
3. A nucleic acid encoding the mutein of claim 2 or a nucleic acid of the same length as the encoding nucleic acid and being completely complementary to the encoding nucleic acid.
4. A vector expressing the mutein of claim 2.
5. A method for preparing a mutant protein of a high-activity factor VIII or VIIIa polypeptide variant Gly710Ala, comprising the following steps:
BDD-hFVIII or full-length FVIII is taken as a template, a PCR fixed-point mutagenesis method is used for introducing mutation sites into wild FVIII, Gly710 amino acid residues are replaced by Ala, and BDD-hFVIII or full-length FVIII eukaryotic expression plasmids which are correctly inserted with Gly710Ala mutation are screened after conversion.
6. Use of a nucleic acid encoding a mutein of a highly active factor viii or villa polypeptide variant Gly710 Ala: use of the nucleic acid of claim 1 or 3 for the preparation of a gene therapy drug comprising linking it to promoter and termination sequences to construct an expression plasmid, gene therapy viral or non-viral vector expressing the mutein of claim 2.
7. The use of a mutein of highly active factor viii or villa polypeptide variant Gly710Ala according to claim 2 wherein: is applied to preparing the recombinant protein therapeutic drug for hemophilia.
8. The use of a mutein of highly active factor viii or villa polypeptide variant Gly710Ala according to claim 2 wherein: the fusion protein is applied to preparing the fusion protein of the polypeptide variant Gly710Ala of the blood coagulation factor VIII or VIII a, and the fusion protein is used for preparing the recombinant protein therapeutic drug for hemophilia.
9. Use according to claim 8, characterized in that: the fusion protein is obtained by fusing one of human albumin, immunoglobulin Fc, transferrin or alpha 1 antitrypsin with the mutant protein of the polypeptide variant Gly710Ala of the blood coagulation factor VIII or VIII a.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114717239A (en) * | 2021-12-30 | 2022-07-08 | 广西医科大学第一附属医院 | High-activity blood coagulation factor VIII or VIII a polypeptide variant Gly710Thr |
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| CN112126636A (en) * | 2020-09-09 | 2020-12-25 | 上海交通大学医学院附属瑞金医院 | High-activity blood coagulation factor XI mutant Ala570Thr |
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| US20030148953A1 (en) * | 1996-04-24 | 2003-08-07 | University Of Michigan | Inactivation resistant factor VIII |
| US20060293238A1 (en) * | 1996-04-24 | 2006-12-28 | University Of Michigan | Inactivation resistant factor VIII |
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| CN114717239A (en) * | 2021-12-30 | 2022-07-08 | 广西医科大学第一附属医院 | High-activity blood coagulation factor VIII or VIII a polypeptide variant Gly710Thr |
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