CN114190999A

CN114190999A - SD rat acupoint catgut embedding sampling method

Info

Publication number: CN114190999A
Application number: CN202111475134.5A
Authority: CN
Inventors: 周芋伶; 张倩; 金园; 何灏龙; 贺芳姿
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-12-03
Filing date: 2021-12-03
Publication date: 2022-03-18

Abstract

The invention relates to the technical field of tests and discloses a SD rat acupoint catgut embedding and taking method which comprises the following steps: s1, preprocessing, namely firstly finding a target acupuncture point before thread burying; s2, marking a square with the length and the width of 1cm by taking a target acupuncture point as a center; s3, preparing a buried wire, and disinfecting after marking to prepare equipment; s4, performing line burying treatment, wherein the burying depth is 0.5cm, drawing a mark on the square marked in the step S2, imagining an acupuncture point in an animal body into a cube, projecting a central cylinder in the cube to the surface of the acupuncture point, performing 45-degree oblique needling on the edge of the marked square when the line is buried, and using a line burying needle with scales, wherein the vertical burying depth is 0.5cm when the burying depth is 0.7 cm; s5, performing a reverse fast cutting method on the basis of the steps; the invention saves the experiment time, improves the experiment efficiency and optimizes the problem that the epidermis and the deep tissue can not be cut off without damage in the traditional method.

Description

SD rat acupoint catgut embedding sampling method

Technical Field

The invention relates to the technical field of tests, in particular to a method for obtaining SD rat acupuncture point catgut implantation materials.

Background

At the initial stage of thread embedding, the needle pierces the skin and embeds catgut in the acupoint to produce the heavy needle feeling of acid bloating, namely 'qi getting', and then the needle feels 'qi flowing to the disease location' along the meridian. The catgut is embedded into the acupuncture points to generate physical and biochemical stimulation to the acupuncture points, so that the stimulation is transmitted into the body through meridians and collaterals, and the effect of treating diseases is finally achieved. Has the characteristics of simple and convenient operation, small wound, strong stimulation, durable action, less treatment frequency and the like, is widely applied clinically, is mainly used for treating diseases of digestive system, respiratory system, nervous system, gynecology, vertebra, joint and the like, and obtains more satisfactory curative effect. The operation of catgut embedding at the acupuncture points on the human body: (1) strictly sterilizing the acupuncture point region from inside to outside by using iodophor disinfectant. (2) The needle-holding catgut embedding operation person wears sterile medical gloves, penetrates a catgut into the needle point of the catgut embedding needle by forceps, tightens the skin by the left hand, quickly penetrates the skin by holding the needle by the right hand, pushes the needle core inwards by the left hand after obtaining qi, draws the needle head outwards by the right hand, leaves the catgut in the subcutaneous tissue or muscle layer of the acupuncture point, and then withdraws the needle; if a special disposable thread burial needle is used, the operation is directly carried out. (3) After the needle is taken out, the local part is pasted at the needle hole by using the band-aid or the special paste, and the needle is taken down after 2 to 4 hours. The treatment course is 7-15 days or 4-6 times according to the disease condition. With the wide application of catgut embedding therapy, the related research thereof is gradually concerned. In recent years, studies on the mechanism of catgut implantation have been endless, and most animals are used as models. When the animal acupuncture point tissue is taken down during research, the tissue cannot be injured in the process, so that the subsequent experiment detection is not influenced. In multiple experiments of the subject group, no standard material taking method is found, and it is a significant problem to take out target tissues embedded in a plurality of experimental SD rats and obtain effective and accurate experimental data. In addition, when animal experiment research is carried out, materials are required to be taken for detection, and the traditional shearing mode is far insufficient for achieving the purpose of protecting subcutaneous cells. Related researches show that the acupoint catgut embedding can regulate synthesis and release of various neurotransmitters, immune cells and cytokines, and regulate enzyme activity, cell surface receptors and disease-related gene expression. In order to take out the line body accurately and minimize the tissue damage, a high-efficiency and convenient material taking method is very necessary.

Disclosure of Invention

Technical problem to be solved

Aiming at the defects of the prior art, the invention provides a method for taking SD rat acupuncture point catgut implantation materials, which solves the problems in the background technology.

(II) technical scheme

In order to achieve the purpose, the invention provides the following technical scheme: a SD rat acupoint catgut embedding material taking method comprises the following steps:

s1, preprocessing, namely firstly finding a target acupuncture point before thread burying;

s2, marking a square with the length and the width of 1cm by taking a target acupuncture point as a center;

s3, preparing a buried wire, and disinfecting after marking to prepare equipment;

s4, processing thread burying, wherein the burying depth is 0.5cm, drawing a mark on the square marked in the step S2, imagining an acupuncture point in the animal body into a cube, projecting a central cylinder in the cube to the surface of the acupuncture point, performing 45-degree oblique needling on the edge of the marked square when the thread is buried, and using a thread burying needle with scales, wherein the perpendicular burying depth is 0.5cm when the burying depth is 0.7 cm.

And S5, performing reverse quick cutting method on the basis of the steps so as to remove the complete tissue.

Preferably, in step S5, the reverse fast cutting method includes: when operating, the rat shank is held down to the left hand, and the GEM cutting knife is held to the right hand and is carried out quick reverse cutting on the square that marks, cuts back, uses ophthalmic scissors to cut on other both sides, and the tissue at the purpose degree of depth is transected simultaneously, and at this moment, complete tissue will be taken off.

(III) advantageous effects

The invention provides a SD rat acupoint catgut embedding and taking method, which has the following beneficial effects:

(1) when the materials are taken, subcutaneous cells can be protected, and the experimental accuracy is improved.

(2) When the invention is used for cutting, the skin and the subcutaneous fascia of a rat are fixed and can not slide, the target tissue is clear, and the tissue damage is small.

(3) The invention saves the experiment time, improves the experiment efficiency and optimizes the problem that the epidermis and the deep tissue can not be cut off without damage in the traditional method.

Drawings

FIG. 1 is a schematic view of the reverse fly-cutting method of the present invention.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

As shown in fig. 1, the present invention provides a technical solution: a SD rat acupoint catgut embedding material taking method comprises the following steps:

And S5, performing a reverse fast cutting method on the basis of the steps, wherein in the operation, the leg of the rat is pressed by the left hand, the GEM cutter knife is held by the right hand to perform fast reverse cutting on the marked square, after cutting, the other two sides are cut by using an ophthalmic scissors, meanwhile, the tissue at the target depth is transversely cut, and at the moment, the complete tissue is taken down.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A SD rat acupoint catgut embedding material taking method is characterized in that: the method comprises the following steps:

2. The method for obtaining materials for SD rat by catgut implantation at acupoint according to claim 1, wherein the method comprises the following steps: in step S5, the reverse fast cutting method includes the specific steps of: when operating, the rat shank is held down to the left hand, and the GEM cutting knife is held to the right hand and is carried out quick reverse cutting on the square that marks, cuts back, uses ophthalmic scissors to cut on other both sides, and the tissue at the purpose degree of depth is transected simultaneously, and at this moment, complete tissue will be taken off.