CN114181853B

CN114181853B - Pseudomarigold strain with remarkable degradation effect on malathion and application thereof

Info

Publication number: CN114181853B
Application number: CN202111468257.6A
Authority: CN
Inventors: 董志扬; 马羚; 王丽; 戴欣
Original assignee: Institute of Microbiology of CAS
Current assignee: Institute of Microbiology of CAS
Priority date: 2021-12-03
Filing date: 2021-12-03
Publication date: 2023-11-24
Anticipated expiration: 2041-12-03
Also published as: CN114181853A

Abstract

The application discloses a pseudomarigold strain with remarkable malathion degradation effect and application thereof. The malathion degrading bacteria are pseudo sea source bacteria (pseudo sea sp.) from deep sea hydrothermal solution, the strain number is R2059-CB1-ML, and the registration number of the malathion degrading bacteria in the China general microbiological culture Collection center is CGMCC No.23970. The strain can degrade malathion, treat seawater pollution and can be used for improving marine environment.

Description

Pseudomarigold strain with remarkable degradation effect on malathion and application thereof

Technical Field

The application relates to a pseudomarigold strain with remarkable degradation effect on malathion and application thereof in the field of biotechnology.

Background

The organic phosphorus compound is an artificially synthesized phosphorus-containing high-toxicity substance and is widely applied to the fields of agriculture, industry and national defense, such as pesticides, flame retardants, plasticizers, chemical weapons and the like. Organophosphorus pesticides (organophosphorus pesticides, OPs) are widely used in agricultural production, and malathion is one of them because of their high efficiency, broad spectrum, low residue, low cost of use, etc.

Malathion is an efficient and highly toxic organophosphorus pesticide and is widely applied to the control of crop insect pests. The accumulation and toxicity of the composition in the environment are the greatest threats to the environment, and are fatal to beneficial insects, snails, micro crustaceans, fish, birds, amphibians and soil microorganisms, and besides, the composition has potential threats to the health of human beings, can inhibit the function of acetylcholinesterase in the body, has different degrees of toxicity to mammals, and acute poisoning can cause muscle spasm, pupil constriction and dyspnea until death, so that research on degradation of malathion is necessary.

With the improvement of life quality and the enhancement of environmental awareness of people, the problem of degrading organophosphorus pesticides is more and more paid attention to, and research on microbial degradation of pesticides begins in the 50 th century of 20 th year, and a large number of experiments prove that microbial degradation is the most important mode of degrading pesticides in the environment. The microorganism has the characteristics of multiple species, rapid variation, easy operation and the like. Strains for degrading malathion, including Pseudomonas alteromonas, escherichia coli, brevundimonas, flavobacterium, arthrobacter, aspergillus and the like, are reported, and no report on degradation of malathion by bacteria of the genus pseudomaria from deep sea is found.

Disclosure of Invention

The application aims to solve the technical problems of degrading malathion in seawater and/or treating marine pollution.

In order to solve the technical problems, the application firstly provides a pseudomaria sp.

The pseudomaritime source bacterium (pseudomaritime sp.) R2059-CB1-ML has a registration number of CGMCC No.23970 in the common microorganism center of China Committee for culture Collection of microorganisms. The strain is preserved in China general microbiological culture collection center (CGMCC) for 11 and 25 days in 2021, and the preservation address is North Chen West Lu No. 1 and No. 3 in the Korean region of Beijing city. Hereinafter, pseudomaria species R2059-CB1-ML is abbreviated.

Pseudomaritime-derived bacteria (pseudomaritime sp.) R2059-CB1-ML was isolated from a deep sea Gu Re liquid sediment in south China sea and was gram-negative bacteria in the form of a rod, and after 24 hours of culture, the strain was streaked out in 2216E solid medium and was circular, pale yellow transparent, smooth in surface and convex. The pseudomaria (pseudothiomarina sp.) R2059-CB1-ML has 16S rDNA shown in sequence 1 in a sequence table.

Any of the following applications of the pseudomaritime bacteria R2059-CB1-ML or/and the metabolite of the pseudomaritime bacteria R2059-CB1-ML also belong to the protection scope of the application:

u1, pseudomaritime bacteria R2059-CB1-ML or/and the application of the metabolite of the pseudomaritime bacteria R2059-CB1-ML in the degradation of organic phosphorus or the preparation of products for degrading the organic phosphorus;

u2, pseudomarigold R2059-CB1-ML or/and the metabolite of the pseudomarigold R2059-CB1-ML are applied to the degradation of malathion or the preparation of a product for degrading malathion;

u3, the pseudomaritime bacteria R2059-CB1-ML or/and the metabolite of the pseudomaritime bacteria R2059-CB1-ML are applied to improving the marine environment or the product for improving the marine environment;

u4, the pseudomaritime bacteria R2059-CB1-ML or/and the metabolite of the pseudomaritime bacteria R2059-CB1-ML are applied to the treatment of seawater pollution or the preparation of products for the treatment of seawater pollution.

In order to solve the technical problems, the application also provides a product, and the product provided by the application contains the pseudomarigold R2059-CB1-ML or/and the metabolite of the pseudomarigold R2059-CB1-ML.

The product can be a microbial agent and a microecological preparation containing the microbial agent.

The product can be specifically any one of the following products:

v1, degrading organic phosphorus products;

v2, degrading malathion products;

v3, improving marine environment;

v4, treating seawater polluted products.

The active ingredients of the product can be pseudoseaborne bacteria R2059-CB1-ML or/and the metabolite of the pseudoseaborne bacteria R2059-CB1-ML, the active ingredients of the product can also contain other biological components or non-biological components, and the other active ingredients of the product can be determined by a person skilled in the art according to the effect of the product.

The product may also include a carrier. The carrier may be a solid carrier or a liquid carrier. The solid carrier is a mineral material and a biological material; the mineral material may be at least one of turf, clay, talc, kaolin, montmorillonite, white carbon, zeolite, silica, and diatomaceous earth; the biological material is at least one of straws, pine shells, rice straws, peanut shells, corn flour, bean flour, starch, turf and animal excrement of various crops; the liquid carrier may be water; in the product, the pseudomarigold R2059-CB1-ML or/and the metabolite of the pseudomarigold R2059-CB1-ML may exist in the form of living cells to be cultured, a fermentation broth of the living cells, a filtrate of the cell culture or a mixture of cells and the filtrate. The dosage form of the product can be various dosage forms, such as liquid, emulsion, suspending agent, powder, granule, wettable powder or water dispersible granule.

Surfactants (such as Tween 20, tween 80, etc.), binders, stabilizers (such as antioxidants), pH regulators, etc. can be added to the product as required.

Any of the following applications of the above products also fall within the scope of the application:

x1, application of the product in degrading organic phosphorus;

x2, the application of the product in degrading malathion;

x3, application of the product in improving marine environment;

x4, the application of the product in treating seawater pollution.

In the above, the metabolite of the pseudomaritime fungus R2059-CB1-ML may be a fermentation broth of the pseudomaritime fungus R2059-CB1-ML. The fermentation broth of the pseudosea source bacteria R2059-CB1-ML can be prepared according to the following method: culturing pseudomarigold R2059-CB1-ML in a liquid fermentation medium, and collecting fermentation broth (containing the pseudomarigold R2059-CB1-ML and substances secreted into the liquid culture medium), wherein the fermentation broth is the metabolite of the pseudomarigold R2059-CB1-ML.

Cultures of the pseudomaritime bacteria R2059-CB1-ML also fall within the scope of the present application. The culture of the pseudomarigold R2059-CB1-ML is a culture obtained by culturing the pseudomarigold R2059-CB1-ML in a microorganism culture medium (e.g., a fermentation broth which contains the pseudomarigold R2059-CB1-ML and a substance secreted into a liquid culture medium, or a culture broth which contains the pseudomarigold R2059-CB1-ML and a substance secreted into a solid culture medium).

The culture of the pseudomaritime fungus R2059-CB1-ML has at least one of the following functions of W1-W4:

w1, degrading organic phosphorus;

w2, degrading malathion;

w3, improving the marine environment;

w4, treating seawater pollution.

The method for culturing the pseudomaritime source bacteria R2059-CB1-ML also belongs to the protection scope of the application.

The method for culturing the pseudomaritime source bacteria R2059-CB1-ML comprises the step of culturing the pseudomaritime source bacteria R2059-CB1-ML in a culture medium.

The method of preparing the product is also within the scope of the present application.

The method for preparing the product provided by the application comprises the step of taking the pseudomarigold R2059-CB1-ML and/or the metabolite of the pseudomarigold R2059-CB1-ML as a component of the product to obtain the product.

In the above method, the product may be a microbial inoculum. The microbial inoculum can be a solid microbial inoculum or a liquid microbial inoculum.

In the method, the pseudomaritime fungus R2059-CB1-ML can be cultured in a fermentation medium to obtain fermentation broth, and the fermentation broth is mixed with a carrier to obtain the liquid microbial inoculum. The fermentation medium may be 2216E liquid medium or inorganic salt liquid medium.

The preparation method of the 2216E liquid culture medium comprises the following steps: 5g tryptone, 1g yeast powder extract, and sterilizing with artificial seawater to 1L 121 deg.C under high pressure and moist heat for 20 min; the preparation method of the artificial seawater comprises the following steps: 23.477g NaCl,3.917g Na ₂ SO ₄ ，4.981g MgCl ₂ ·6H ₂ O，1.102g CaCl ₂ ，193mg NaHCO ₃ ，664mg KCl，6mg KBr，20mg H ₃ BO ₃ ，24mg SrCl ₂ 3mg NaF, ddH ₂ O is fixed to volume to 1L.

The composition of the inorganic salt liquid culture medium can be as follows: 18g sea salt, 1g (NH) ₄ ) ₂ SO ₄ 0.3g glucose, 0.4g yeast extract, ddH ₂ O is fixed to 1L, and the mixture is sterilized by high-pressure moist heat at 121 ℃ for 20min.

Experiments prove that the pseudomaritime fungus R2059-CB1-ML can hydrolyze malathion in organic phosphorus, can treat seawater pollution, and can be used for improving marine environment. The pseudomaritime source bacteria R2059-CB1-ML is safe to human and livestock, has no environmental pollution problem, has simple culture condition and easy preservation, and is suitable for development and application.

Description of biological Material preservation

Classification naming of biological materials: pseudosea source bacteria

Latin Wen Xueming of biological material: pseudidiomarina sp.

Strain number of biological material: R2059-CB1-ML

The preservation units are fully named: china general microbiological culture Collection center (China Committee for culture Collection of microorganisms)

The preservation unit is abbreviated as: CGMCC

Address: beijing city, chaoyang area, north Chenxi Lu No. 1 and 3

Preservation date: 2021, 11, 25

Preservation number: CGMCC No.23970

Drawings

FIG. 1 is a photograph of strain R2059-CB1-ML after streaking in 2216E solid medium for 24 hours.

FIG. 2 is a photograph showing the result of gram staining of the strain R2059-CB1-ML.

FIG. 3 is a photograph of a scanning electron microscope of strain R2059-CB1-ML after 10 hours of culture in 2216E liquid medium.

FIG. 4 is a photograph of a scanning electron microscope of strain R2059-CB1-ML after 48 hours of culture in a liquid medium containing malathion in an inorganic salt.

FIG. 5 is the result of alignment of the 16S sequence of strain R2059-CB1-ML in NCBI library.

FIG. 6 shows the results of ANI analysis of the whole genome sequence of strain R2059-CB1-ML and the genome sequence of model strain Pseudidiomarina homiensis strain PO-M2 (NR 043732.1).

FIG. 7 shows the results of DDH analysis of the whole genome sequence of strain R2059-CB1-ML and the genome sequence of model strain Pseudidiomarina homiensis strain PO-M2 (NR 043732.1).

FIG. 8 shows the HPLC detection result of malathion after 12h of culture of the strain R2059-CB1-ML (labeled CB 1) in an inorganic salt medium.

FIG. 9 shows the HPLC detection result of malathion after 18h of culture of the strain R2059-CB1-ML (labeled CB 1) in an inorganic salt medium.

FIG. 10 shows the HPLC detection result of malathion after culturing strain R2059-CB1-ML (labeled CB 1) in inorganic salt medium for 24 hours.

FIG. 11 shows HPLC detection results of malathion after culturing strain R2059-CB1-ML (labeled CB 1) in inorganic salt medium for 30 hours.

FIG. 12 shows the HPLC detection result of malathion after culturing strain R2059-CB1-ML (labeled CB 1) in inorganic salt medium for 40 hours.

FIG. 13 shows the change dynamics of malathion degradation rate when strain R2059-CB1-ML is cultured in inorganic salt medium.

Detailed Description

The following detailed description of the application is provided in connection with the accompanying drawings that are presented to illustrate the application and not to limit the scope thereof. The examples provided below are intended as guidelines for further modifications by one of ordinary skill in the art and are not to be construed as limiting the application in any way.

The experimental methods in the following examples are conventional methods unless otherwise specified. The materials, reagents, etc. used in the examples described below are all conventional biochemical reagents, unless otherwise specified, and are commercially available.

The quantitative tests in the following examples were all performed in triplicate, and the results were averaged.

In the following examples, the preparation methods of the solutions and the culture media used are as follows:

1) Artificial seawater: 23.477g NaCl,3.917g Na ₂ SO ₄ ，4.981g MgCl ₂ ·6H ₂ O，1.102g CaCl ₂ ，193mg NaHCO ₃ ，664mg KCl，6mg KBr，20mg H ₃ BO ₃ ，24mg SrCl ₂ 3mg NaF, ddH ₂ O is fixed to volume to 1L.

2) 2216E liquid medium: 5g tryptone, 1g yeast extract, and sterilizing with artificial seawater to 1L at 121deg.C under high pressure and moist heat for 20min.

3) 2216E solid medium: 5g of tryptone, 1g of yeast powder extract and 20g of agar, and sterilizing with artificial seawater to 1L at 121 ℃ under high pressure and moist heat for 20min.

4) Inorganic salt liquid medium: 18g sea salt, 1g (NH) ₄ ) ₂ SO ₄ 0.3g glucose, 0.4g yeast extract, ddH ₂ O is fixed to 1L, and the mixture is sterilized by high-pressure moist heat at 121 ℃ for 20min.

5) Malathion mother liquor: 0.1g of malathion standard is dissolved in 2mL of dimethyl sulfoxide (DMSO) solution to obtain a dimethyl sulfoxide solution of malathion, wherein the concentration of malathion is 50g/L.

Example 1 screening and enrichment of Marine bacteria malathion degrading bacteria

1. Isolation of pseudomaritime-derived fungus (pseudoiodiomarina sp.) R2059-CB1-ML CGMCC No.23970

The pseudomaritime source fungus (pseudomaritime sp.) R2059-CB1-ML CGMCC No.23970 is separated from a deep sea paleo-thermal liquid sample of the south sea. The south sea deep sea archaea liquid sample is sampled in 5 months in 2018, the sampling place is south sea N15.034363, E116.522965, and the sample is sediment sample with water depth of 988 m.

Streaking screening and purification are carried out by adopting 2216E solid culture medium plates at the temperature of 30 ℃. The purified strains are respectively connected into triangular flasks filled with 2216E liquid culture medium, and shaking culture is carried out for 10 hours at 37 ℃ and 200rpm, so as to obtain fermentation liquor cultured by the 2216E liquid culture medium for standby.

After streaking for 24 hours in 2216E solid medium, the strain is round, light yellow transparent, smooth in surface and convex (see FIG. 1). Gram staining of R2059-CB1-ML is red (see figure 2), gram-negative bacteria, and the strain is in a rod shape, an electron microscope scanning photo of R2059-CB1-ML after 10h culture in 2216E liquid culture medium is shown in figure 3, and an electron microscope scanning photo of R2059-CB1-ML after 48h culture in malathion-containing inorganic salt liquid culture medium is shown in figure 4.

The 16S rDNA of the strain R2059-CB1-ML has the nucleotide sequence of the sequence 1 in the sequence table, the comparison result in NCBI library is shown in figure 5, the similarity of the R2059-CB1-ML strain and Pseudidiomarina homiensis strain PO-M2 (NR 043732.1) is 99.59%, and the whole genome sequencing of the R2059-CB1-ML is carried out.

The strain R2059-CB1-ML whole genome sequence was analytically aligned with the model strain Pseudidiomarina homiensis strain PO-M2 (NR 043732.1) genome sequence published in NCBI (GCF_003987225.1_ASM398722 v 1_genomic), with ANI results of 94.06% (less than 95% of the critical value of the different interspecies ANI, see FIG. 6 in particular), while the DDH data results were: 56.1% (less than 70%, see in particular FIG. 7), and thus, the strain R2059-CB1-ML is not of the same species as Pseudidiomarina homiensis.

The strain R2059-CB1-ML is identified as a new species by the morphological characteristics, the culture characteristics, the physiological and biochemical characteristics and the sequence alignment, and is pseudomaria sp. Pseudomaritime source bacteria (pseudomariina sp.) R2059-CB1-ML was deposited at the China general microbiological culture collection center (CGMCC) for 11 and 25 days in 2021, the deposited address is the North Chen Xiyu No. 1, 3 in the Korean region of Beijing city, and the registration number of the pseudomaritime source bacteria at the China general microbiological culture collection center is CGMCC No.23970. Hereinafter, pseudomaria species R2059-CB1-ML is abbreviated.

Example 2 degradation of malathion by Marine pseudobacteria R2059-CB1-ML

Detecting OD (OD) in ultraviolet spectrophotometer by fermentation broth of pseudomarigold R2059-CB1-ML strain cultured in 2216E liquid culture medium stored in example 1 _600nm OD is collected _600nm The fermentation broth of=4 (blank with 2216E liquid medium) was centrifuged at 3500rpm at room temperature for 8min, the supernatant was discarded, resuspended in an equal volume of inorganic salt liquid medium, and washed 1-2 times to obtain a resuspended fermentation broth. Two treatments, an experimental group and a control group, were set as follows:

experimental group: the above-mentioned resuspended broth was added to 10mL of an inorganic salt liquid medium at a ratio of 3% (v/v), and malathion mother liquor was finally added thereto at a ratio of 1% (v/v). And under the condition of 28 ℃, carrying out shading vibration culture for 12 hours, 18 hours, 24 hours, 30 hours and 40 hours at 200rpm to obtain inorganic salt fermentation liquor of R2059-CB1-ML strains with different fermentation time lengths, and taking the inorganic salt fermentation liquor as experimental group solutions with different fermentation time lengths for standby.

Control group: malathion stock solution was added in a ratio of 1% (v/v) to 10mL of inorganic salt liquid medium. And (3) carrying out shading shake culture at 200rpm for 12 hours, 18 hours, 24 hours, 30 hours and 40 hours at 28 ℃ to sample, wherein the sample is used as a control group solution with different fermentation time lengths for standby.

Respectively adding equal volumes of n-hexane into the experimental group solution and the control group solution, sufficiently shaking for 30s, standing for 2-3h at room temperature, sucking 1mL of an upper organic phase solution, performing rotary evaporation at 25 ℃ for 10min, adding 1mL of a water-acetonitrile mixed solution (1:1), resuspending, and filtering to obtain solutions serving as an experimental group solution to be detected and a control group solution to be detected respectively for later use.

And detecting the content of malathion in the test liquid of the experimental group and the test liquid of the control group by utilizing HPLC.

HPLC detection conditions: using a C18 reverse-direction analytical column (Eclipse plus C18,4.6mm×100mm, dp=35 μm) with a suitable guard column and uv detector, acetonitrile was used: water (50:50) was used as the mobile phase at a flow rate of 1mL/min,10min, an injection volume of 10. Mu.L, and a retention time of: and 7.28min, and detecting the content of malathion. In HPLC analysis, malathion (CDCT-C14710000) is taken as a standard substance, the malathion is quantitatively analyzed according to the retention time qualitative property of the standard substance and a standard curve method (external standard method), and the malathion degradation rate is calculated according to the following formula:

malathion degradation rate = (content of malathion before degradation-content of malathion after degradation)/content of malathion before degradation x 100%

After 12h of culture in inorganic salt culture medium, HPLC detection results are shown in FIG. 8, characteristic peaks appear in malathion in a control group at retention time of 7.28min, characteristic peaks are detected in an experimental group at retention time of 7.281min, and the degradation rate of malathion in the experimental group is 65.2% according to peak area calculation, and the calculation formula is as follows:

malathion degradation rate = (content of malathion before reaction-content of malathion after reaction)/content of malathion before reaction x 100%

After 18h incubation in inorganic salt medium, HPLC detection results are shown in FIG. 9, showing characteristic peaks of malathion in control group and experimental group at retention time of 7.286min, and according to peak area calculation, the degradation rate of malathion in experimental group is 91.1%.

After 24h of culture in inorganic salt culture medium, HPLC detection results are shown in FIG. 10, which shows that characteristic peaks appear in malathion in a control group at retention time of 7.274min, characteristic peaks are detected in an experimental group at retention time of 7.28min, and the degradation rate of malathion in the experimental group is 96.8% according to peak area calculation.

After 30h of culture in inorganic salt culture medium, HPLC detection results are shown in FIG. 11, which shows that characteristic peaks appear in malathion in a control group at retention time of 7.271min, characteristic peaks are detected in an experimental group at retention time of 7.28min, and the degradation rate of malathion in the experimental group is 98.5% according to peak area calculation.

After 40h of culture in inorganic salt medium, HPLC detection results are shown in FIG. 12, which shows that characteristic peaks appear in malathion in the control group at retention time of 7.282min, and no characteristic peaks are detected in the experimental group at retention time of 7.28min, namely after 40h of culture, the degradation rate of malathion in the experimental group is 100%.

The dynamic change of malathion degradation rate of the strain R2059-CB1-ML when the strain is cultured in an inorganic salt culture medium is shown in figure 13.

In conclusion, the pseudomaritime fungus R2059-CB1-ML can be used as a bacterial strain living in seawater, can degrade malathion, and can be used for treating marine pollution.

The present application is described in detail above. It will be apparent to those skilled in the art that the present application can be practiced in a wide range of equivalent parameters, concentrations, and conditions without departing from the spirit and scope of the application and without undue experimentation. While the application has been described with respect to specific embodiments, it will be appreciated that the application may be further modified. In general, this application is intended to cover any variations, uses, or adaptations of the application following, in general, the principles of the application and including such departures from the present disclosure as come within known or customary practice within the art to which the application pertains. The application of some of the basic features may be done in accordance with the scope of the claims that follow.

Sequence listing

<110> institute of microorganisms at national academy of sciences

<120> a pseudomarigold strain with remarkable malathion degradation effect and application thereof

<130> GNCSY213344

<160> 1

<170> SIPOSequenceListing 1.0

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<211> 1507

<212> DNA

<213> pseudomarine bacteria (pseudopimarina sp.)

<400> 1

agagtttgat catggctcag attgaacgct ggcggcaggc ctaacacatg caagtcgagc 60

ggtaacagag agaagcttgc ttctttgctg acgagcggcg gacgggtgag taatacttgg 120

gaacttgcct ttaggagggg gacaaccacg ggaaactgtg gctaataccg cataatgtct 180

acggaccaaa gcaggggatc ttcggacctt gtacctaaag atgggcccaa gcgagattag 240

ctagttggtg gggtaaaggc tcaccaaggc gacgatctct agctgttctg agaggatgat 300

cagccacact gggactgaga cacggcccag actcctacgg gaggcagcag tggggaatat 360

tgcacaatgg gggaaaccct gatgcagcca tgccgcgtgt gtgaagaagg ccttcgggtt 420

gtaaagcact ttcagtggtg aggaaggcca tacacttaat acgtgtgtgg attgacgtta 480

accacagaag aagcaccggc taactccgtg ccagcagccg cggtaatacg gagggtgcga 540

gcgttaatcg gaattactgg gcgtaaagcg tacgtaggcg gcttgttaag caagatgtga 600

aagccccggg ctcaacctgg gaatggcatt ttgaactggc aagctcgagt tctgaagagg 660

gtggtagaat ttcctgtgta gcggtgaaat gcgtagatat aggaaggaat accggtggcg 720

aaggcggcca cctggtcaga aactgacgct gaggtacgaa agcgtgggga gcaaacagga 780

ttagataccc tggtagtcca cgccgtaaac gatgtcaact agttgttcgt cttataaaca 840

aggtgagtaa cgcagctaac gcactaagtt gaccgcctgg ggagtacggc cgcaaggtta 900

aaactcaaat gaattgacgg gggcccgcac aagcggtgga gcatgtggtt taattcgatg 960

caacgcgaag aaccttacca tcccttgaca tccagtgaac tttctagaga tagattggtg 1020

ccttcgggaa cactgagaca ggtgctgcat ggctgtcgtc agctcgtgtt gtgagatgtt 1080

gggttaagtc ccgcaacgag cgcaaccctt atcctcagtt gccagcacat aatggtggga 1140

actctgtgga gactgccggt gataaaccgg aggaaggtgg ggacgacgtc aagtcatcat 1200

ggcccttacg ggatgggcta cacacgtgct acaatggcgc gtacaatggg aagcaagcta 1260

gcgatagtaa gcggatctca aaaagcgcgt cgtagtccgg atcggagtct gcaactcgac 1320

tccgtgaagt cggaatcgct agtaatcgtg gatcagaatg ccacggtgaa tacgttcccg 1380

ggccttgtac acaccgcccg tcacaccatg ggagtgggct gcaccagaag tggatagttt 1440

aaccttcggg agaacgttca ccacggtgtg gttcatgact ggggtgaagt cgtaacaagg 1500

tagccgt 1507

Claims

1. The pseudosea source bacterium is characterized in that: the pseudomaritime bacteria are pseudomaritime bacteria (pseudomaritime sp.), the strain number is R2059-CB1-ML, and the registration number of the pseudomaritime bacteria in the common microorganism center of China Committee for culture Collection of microorganisms is CGMCC No.23970.

2. The use of a pseudomaritime source bacterium according to claim 1 in the degradation of malathion or in the preparation of a product for the degradation of malathion.

3. A malathion degrading product characterized by: a pseudomaritime-derived bacterium R2059-CB1-ML according to claim 1.

4. Use of the product of claim 3 for degrading malathion.

5. The method for culturing the pseudomaritime-derived bacterium R2059-CB1-ML according to claim 1, wherein: comprising the step of culturing the pseudomaritime source bacteria in a medium.

6. A method of preparing the product of claim 3, characterized by: comprising the step of taking the pseudomaritime fungus R2059-CB1-ML as a component of a product to obtain the product.

7. The method of claim 6, wherein: the product is a liquid microbial agent or a solid microbial agent.