CN114181853A

CN114181853A - Pseudomarine bacterial strain with remarkable degradation effect on malathion and application thereof

Info

Publication number: CN114181853A
Application number: CN202111468257.6A
Authority: CN
Inventors: 董志扬; 马羚; 王丽; 戴欣
Original assignee: Institute of Microbiology of CAS
Current assignee: Institute of Microbiology of CAS
Priority date: 2021-12-03
Filing date: 2021-12-03
Publication date: 2022-03-15
Anticipated expiration: 2041-12-03
Also published as: CN114181853B

Abstract

The invention discloses a pseudomarine bacterial strain with a remarkable effect of degrading malathion and application thereof. The malathion degrading bacteria are pseudo-marine bacteria (pseudoiomarina sp.) with deep-sea hydrothermal sources, the strain number of the malathion degrading bacteria is R2059-CB1-ML, and the registration number of the malathion degrading bacteria in the China general microbiological culture Collection center is CGMCC No. 23970. The strain can degrade malathion, treat seawater pollution, and can be used for marine environment improvement.

Description

Pseudomarine bacterial strain with remarkable degradation effect on malathion and application thereof

Technical Field

The invention relates to a pseudomarine bacterial strain with obvious degradation effect on malathion in the field of biotechnology and application thereof.

Background

The organic phosphorus compound is an artificially synthesized phosphorus-containing high-toxicity substance and is widely applied to agricultural, industrial and national defense fields such as pesticides, flame retardants, plasticizers, chemical weapons and the like. Organophosphorus Pesticides (OPs) are widely used in agricultural production due to their characteristics of high efficiency, broad spectrum, low residue, low use cost, and the like, and malathion is one of them.

Malathion is an efficient and highly toxic organophosphorus pesticide and is widely applied to prevention and control of crop pests. Its accumulation in the environment and its toxicity are the biggest threats to the environment, and at the same time, it is fatal to beneficial insects, snails, crustaceans, fishes, birds, amphibians and soil microorganisms, besides having potential threats to human health, it can inhibit the function of acetylcholinesterase in vivo, and has different degrees of toxicity to mammals, acute poisoning can cause muscle spasm, pupil contraction, dyspnea until death, so the research of degrading malathion is necessary.

With the improvement of the quality of life and the enhancement of environmental awareness of people, the problem of degrading organophosphorus pesticides is more and more concerned by people, the research on the microbial degradation of pesticides starts in the 50 th 20 th century, and a large number of experiments prove that the microbial degradation is the most important mode for degrading pesticides in the environment. The microorganism has the characteristics of multiple species, quick mutation, easy operation and the like. The prior reported strains for degrading malathion comprise alternaria pseudomonads, escherichia coli, brevundimonas sp, flavobacterium, arthrobacterium, aspergillus and the like, and no report about the degradation of the malathion by the bacteria of the genus pseudomarine from deep sea is found.

Disclosure of Invention

The invention aims to solve the technical problem of how to degrade malathion in seawater and/or how to treat marine pollution.

In order to solve the technical problem, the invention firstly provides a pseudomarine strain (pseudomarine sp) R2059-CB 1-ML.

The pseudo-marine bacteria (pseudomoniina sp.) R2059-CB1-ML has the registration number of CGMCC No.23970 in the common microorganism center of China Committee for culture Collection of microorganisms. The strain is preserved in China general microbiological culture Collection center (CGMCC for short) at 11 months and 25 days in 2021, and the preservation address is No. 3 Xilu No. 1 Beijing, Chaoyang, North Chen, China. Hereinafter, the strain is abbreviated as pseudomarine bacterium R2059-CB 1-ML.

Pseudomarine bacteria (pseudomarine sp.) R2059-CB1-ML is separated from ancient hydrothermal sediment in deep sea in south sea, is gram-negative bacteria, is in a rod-shaped strain shape, is subjected to streak separation in 2216E solid culture medium, and is circular, transparent in light yellow and smooth and convex in surface after being cultured for 24 hours. Pseudomarine bacteria (pseudomarine sp.) R2059-CB1-ML has the 16S rDNA shown in the sequence 1 in the sequence table.

The following applications of the metabolite of the pseudomarine bacteria R2059-CB1-ML or/and the pseudomarine bacteria R2059-CB1-ML also belong to the protection scope of the invention:

the application of U1, pseudo-marine bacteria R2059-CB1-ML or/and metabolites of pseudo-marine bacteria R2059-CB1-ML in degrading organophosphorus or in preparing products for degrading organophosphorus;

the application of metabolites of U2, Pseudomarine bacteria R2059-CB1-ML or/and Pseudomarine bacteria R2059-CB1-ML in degrading malathion or preparing products for degrading the malathion;

the application of metabolites of U3, pseudomarine bacteria R2059-CB1-ML or/and pseudomarine bacteria R2059-CB1-ML in the improvement of marine environment or the application in the preparation of products for improving marine environment;

the application of metabolites of U4, Pseudomarine bacteria R2059-CB1-ML or/and Pseudomarine bacteria R2059-CB1-ML in the treatment of seawater pollution or the preparation of products for treating seawater pollution.

In order to solve the technical problems, the invention also provides a product which contains the metabolite of the pseudo-marine bacteria R2059-CB1-ML or/and the pseudo-marine bacteria R2059-CB 1-ML.

The product can be a microbial inoculum and a microecological preparation containing the microbial inoculum.

The product can be any one of the following products:

v1, products for degrading organic phosphorus;

v2, a product for degrading malathion;

v3, a product for improving marine environment;

v4, processing products polluted by seawater.

The active ingredient of the product can be metabolites of the pseudomarine bacteria R2059-CB1-ML or/and the pseudomarine bacteria R2059-CB1-ML, and the active ingredient of the product can also contain other biological ingredients or non-biological ingredients, and the other active ingredients of the product can be determined by the technicians in the field according to the effect of the product.

The product may also include a carrier. The carrier may be a solid carrier or a liquid carrier. The solid carrier is a mineral material or a biological material; the mineral material may be at least one of grass peat, clay, talc, kaolin, montmorillonite, white carbon, zeolite, silica, and diatomaceous earth; the biological material is at least one of straws, pine shells, rice straws, peanut shells, corn flour, bean flour, starch, grass peat and animal manure of various crops; the liquid carrier can be water; in the product, the metabolite of the pseudomarine bacteria R2059-CB1-ML or/and the pseudomarine bacteria R2059-CB1-ML can exist in the form of cultured living cells, fermentation liquor of the living cells, filtrate of the cell culture or mixture of the cells and the filtrate. The preparation formulation of the product can be various preparation formulations, such as liquid, emulsion, suspending agent, powder, granules, wettable powder or water dispersible granules.

According to the requirement, the product can also be added with surfactant (such as Tween 20, Tween 80, etc.), binder, stabilizer (such as antioxidant), pH regulator, etc.

Any of the following applications of the above products also fall within the scope of the present invention:

x1, the use of said product for degrading organic phosphorus;

x2, the use of said product in degrading malathion;

x3, use of said product in modifying the marine environment;

x4, use of said product in the treatment of seawater pollution.

As described above, the metabolite of the Pseudomarine bacterium R2059-CB1-ML can be a fermentation broth of the Pseudomarine bacterium R2059-CB 1-ML. The fermentation liquor of the pseudomarine bacteria R2059-CB1-ML can be prepared according to the following method: culturing the pseudomarine bacteria R2059-CB1-ML in a liquid fermentation culture medium, and collecting a fermentation liquid (containing the pseudomarine bacteria R2059-CB1-ML and substances secreted into the liquid culture medium), wherein the fermentation liquid is a metabolite of the pseudomarine bacteria R2059-CB 1-ML.

A culture of the Pseudomarine bacteria R2059-CB1-ML also belongs to the protection scope of the invention. The culture of the pseudomarine bacteria R2059-CB1-ML is a substance obtained by culturing the pseudomarine bacteria R2059-CB1-ML in a microorganism culture medium (such as a substance containing the pseudomarine bacteria R2059-CB1-ML and secreted into a liquid culture medium, namely a fermentation broth, or such as a substance containing the pseudomarine bacteria R2059-CB1-ML and secreted into a solid culture medium).

The culture of the above pseudomarine bacterium R2059-CB1-ML has at least one function of the following W1-W4:

w1, degrading organic phosphorus;

w2, degrading malathion;

w3, modified marine environment;

w4, treating seawater pollution.

The method for culturing the pseudomarine bacteria R2059-CB1-ML also belongs to the protection scope of the invention.

The method for culturing the pseudomarine bacteria R2059-CB1-ML comprises the step of culturing the pseudomarine bacteria R2059-CB1-ML in a culture medium.

The method for preparing the product also belongs to the protection scope of the invention.

The method for preparing the product comprises the step of taking the pseudomarine bacteria R2059-CB1-ML and/or the metabolite of the pseudomarine bacteria R2059-CB1-ML as the components of the product to obtain the product.

In the above method, the product may be a microbial inoculum. The microbial inoculum can be a solid microbial inoculum or a liquid microbial inoculum.

In the method, the pseudomarine bacteria R2059-CB1-ML can be cultured in a fermentation medium to obtain fermentation liquor, and the fermentation liquor is mixed with a carrier to obtain the liquid microbial inoculum. The fermentation medium can be 2216E liquid medium or inorganic salt liquid medium.

The 2216E liquid culture medium can be prepared by the following steps: 5g of tryptone and 1g of yeast powder extract, and carrying out high-pressure damp-heat sterilization for 20min at the constant volume of 1L and 121 ℃ by using artificial seawater; the preparation method of the artificial seawater comprises the following steps: 23.477g NaCl, 3.917g Na₂SO₄，4.981g MgCl₂·6H₂O，1.102g CaCl₂，193mg NaHCO₃，664mg KCl，6mg KBr，20mg H₃BO₃，24mg SrCl₂3mg NaF in ddH₂And O is metered to 1L.

The inorganic salt liquid culture medium can be composed of: 18g of sea salt, 1g of (NH)₄)₂SO₄0.3g glucose, 0.4g yeast extract in ddH₂O is added to the solution to be constant volume of 1L, and the solution is sterilized by high pressure and moist heat at 121 ℃ for 20 min.

Experiments prove that the pseudomarine bacteria R2059-CB1-ML can hydrolyze malathion in organophosphorus, can treat seawater pollution and can be used for improving marine environment. The pseudo-marine bacteria R2059-CB1-ML is safe to human and livestock, has no problem of environmental pollution, simple culture conditions, easy storage and is suitable for development and application.

Biological material preservation instructions

Taxonomic nomenclature of biological materials: pseudomarine bacteria

Latin literature name of biomaterial: pseudomonina sp.

Strain number of biological material: R2059-CB1-ML

The preservation unit is called as follows: china general microbiological culture Collection center

The preservation unit is abbreviated as: CGMCC (China general microbiological culture Collection center)

Address: xilu No. 1 Hospital No. 3 of Beijing market facing Yang district

The preservation date is as follows: 11/25/2021

The preservation number is: CGMCC No.23970

Drawings

FIG. 1 is a photograph of strain R2059-CB1-ML after streaking on 2216E solid medium for 24 hours.

FIG. 2 is a photograph showing the gram staining results of the strain R2059-CB 1-ML.

FIG. 3 is an electron microscope scanning photograph of the strain R2059-CB1-ML in 2216E liquid medium after 10h of culture.

FIG. 4 is an electron microscope scanning photograph of the strain R2059-CB1-ML after culturing for 48h in an inorganic salt liquid medium containing malathion.

FIG. 5 shows the results of the alignment of the 16S sequence of strain R2059-CB1-ML in the NCBI library.

FIG. 6 shows the ANI analysis results of the whole genome sequence of the strain R2059-CB1-ML and the genome sequence of the model strain Pseudogenia hominiss strain PO-M2(NR 043732.1).

FIG. 7 shows the DDH analysis results of the whole genome sequence of the strain R2059-CB1-ML and the genome sequence of the model strain Pseudogenia hominiss strain PO-M2(NR 043732.1).

FIG. 8 shows the HPLC detection results of malathion after 12h of culture of strain R2059-CB1-ML (labeled as CB1) in mineral salts medium.

FIG. 9 shows the HPLC detection of malathion after 18h of incubation of strain R2059-CB1-ML (labeled as CB1) in mineral salts medium.

FIG. 10 shows the HPLC detection of malathion after 24h of culture of strain R2059-CB1-ML (labeled as CB1) in mineral salts medium.

FIG. 11 shows the HPLC detection results of malathion after 30h of culture of strain R2059-CB1-ML (labeled as CB1) in mineral salts medium.

FIG. 12 shows the HPLC detection results of malathion after 40h culture of strain R2059-CB1-ML (labeled as CB1) in mineral salts medium.

FIG. 13 is the dynamic change of the degradation rate of malathion when the strain R2059-CB1-ML is cultured in mineral salts medium.

Detailed Description

The present invention is described in further detail below with reference to specific embodiments, which are given for the purpose of illustration only and are not intended to limit the scope of the invention. The examples provided below serve as a guide for further modifications by a person skilled in the art and do not constitute a limitation of the invention in any way.

The experimental procedures in the following examples are conventional unless otherwise specified. Materials, reagents and the like used in the following examples are all conventional biochemical reagents and are commercially available unless otherwise specified.

In the quantitative tests in the following examples, three replicates were set up and the results averaged.

In the following examples, the preparation of the solutions and media used is specifically as follows:

1) artificial seawater: 23.477g NaCl, 3.917g Na₂SO₄，4.981g MgCl₂·6H₂O，1.102g CaCl₂，193mg NaHCO₃，664mg KCl，6mg KBr，20mg H₃BO₃，24mg SrCl₂3mg NaF in ddH₂And O is metered to 1L.

2)2216E liquid medium: 5g of tryptone and 1g of yeast powder extract, adding artificial seawater to a constant volume of 1L, and performing high-pressure moist heat sterilization at 121 ℃ for 20 min.

3)2216E solid Medium: 5g of tryptone, 1g of yeast powder extract and 20g of agar, adding artificial seawater to a constant volume of 1L, and performing high-pressure moist heat sterilization at 121 ℃ for 20 min.

4) Inorganic salt liquid culture medium: 18g of sea salt, 1g of (NH)₄)₂SO₄0.3g glucose, 0.4g yeast extract in ddH₂O is added to the solution to be constant volume of 1L, and the solution is sterilized by high pressure and moist heat at 121 ℃ for 20 min.

5) Malathion mother liquor: dissolving 0.1g of malathion standard substance in 2mL of dimethyl sulfoxide (DMSO) solution to obtain the dimethyl sulfoxide solution of the malathion, wherein the concentration of the malathion is 50 g/L.

Example 1 screening and enrichment of Marine bacteria Malathion degrading bacteria

Separation of pseudo-marine bacteria (pseudomarine sp.) R2059-CB1-ML CGMCC No.23970

Pseudomarine bacteria (pseudomarine sp.) R2059-CB1-ML CGMCC No.23970 is isolated from ancient hydrothermal samples in deep sea in south sea. The ancient hydrothermal sample of the deep sea of the south sea is sampled in 5 months of 2018 at the sampling site of N15.034363 and E116.522965 of the south sea, and is a sediment sample with the water depth of 988 m.

Streaking, screening and purifying by 2216E solid medium plate at 30 deg.C. The purified strains are respectively inoculated into a triangular flask containing 2216E liquid culture medium, and are subjected to shaking culture at 37 ℃ and 200rpm for 10 hours to obtain fermentation liquor cultured by the 2216E liquid culture medium for later use.

After R2059-CB1-ML was streaked on 2216E solid medium for 24 hours, the strain was round, light yellow and transparent, and had a smooth and convex surface (see FIG. 1). The gram stain of R2059-CB1-ML is red (see figure 2), is a gram-negative bacterium, the strain morphology is rod-shaped, the electron microscope scanning photo of R2059-CB1-ML after being cultured in 2216E liquid medium for 10h is shown in figure 3, and the electron microscope scanning photo of R2059-CB1-ML after being cultured in malathion-containing inorganic salt liquid medium for 48h is shown in figure 4.

The 16S rDNA of the strain R2059-CB1-ML has the nucleotide sequence of the sequence 1 in the sequence table, the result of alignment in the NCBI library is shown in figure 5, the similarity of the strain R2059-CB1-ML and pseudoididomannia homiensis strain PO-M2(NR 043732.1) is 99.59%, and then R2059-CB1-ML whole genome sequencing is carried out.

When the whole genome sequence of the strain R2059-CB1-ML was analyzed and compared with the genome sequence of the model strain Pseudomonas hominiss strain PO-M2(NR 043732.1) published in NCBI (GCF-003987225.1-ASM 398722v 1-genomic), the ANI result was 94.06% (less than the critical value 95% of ANI between different species, see FIG. 6 in particular), and the DDH data result was: 56.1% (less than 70%, see in particular FIG. 7), the strain R2059-CB1-ML is therefore not of the same species as Pseudodiomarina homiensis.

The strain R2059-CB1-ML is identified to belong to a new species, namely, pseudo-marine bacteria (pseudomarine sp) through the morphological characteristics, the culture characteristics, the physiological and biochemical characteristics and the sequence alignment. Pseudo-marine bacteria (pseudomoniina sp.) R2059-CB1-ML has been deposited in China general microbiological culture Collection center (CGMCC) at 25.11.20.2021 at the location of Beijing province No. 1 North Chen West Lu, No. 3, and the accession number of the China general microbiological culture Collection center is CGMCC No. 23970. Hereinafter, the strain is abbreviated as pseudomarine bacterium R2059-CB 1-ML.

Example 2 degradation of Malathion by Pseudomarine bacteria R2059-CB1-ML

OD detection was performed in an ultraviolet spectrophotometer on a fermentation broth obtained by culturing the strain of Pseudomarine organism R2059-CB1-ML in the 2216E liquid medium stored in example 1_600nmCollecting the OD_600nmThe fermentation broth of 4 (2216E liquid medium as blank) was centrifuged at 3500rpm for 8min at room temperature, the supernatant was discarded, resuspended in an equal volume of inorganic salt liquid medium, and washed 1-2 times to obtain a resuspended fermentation broth. Two treatments, experimental and control, were set as follows:

experimental groups: the resuspended fermentation broth was added to 10mL of an inorganic salt liquid medium at a ratio of 3% (v/v), and finally, a mother liquor of malathion was added thereto at a ratio of 1% (v/v). Under the condition of 28 ℃, carrying out light-shielding shaking culture for 12h, 18h, 24h, 30h and 40h at 200rpm to obtain inorganic salt fermentation liquor of the R2059-CB1-ML strain with different fermentation durations, and using the inorganic salt fermentation liquor as experimental group solutions with different fermentation durations for later use.

Control group: a malathion mother liquor was added to 10mL of an inorganic salt liquid medium at a ratio of 1% (v/v). The samples were taken after 12h, 18h, 24h, 30h and 40h of the shaking culture at 200rpm and 28 ℃ in the dark, and the samples were used as control solutions for different fermentation periods.

Adding equal volume of n-hexane into the experimental group solution and the control group solution respectively, shaking for 30s, standing for 2-3h at room temperature, absorbing 1mL of upper organic phase solution, performing rotary evaporation for 10min at 25 ℃, adding 1mL of water-acetonitrile mixed solution (1: 1) for re-suspension, and filtering, wherein the solutions are respectively used as the experimental group solution to be detected and the control group solution to be detected for later use.

And detecting the contents of malathion in the test solution of the experimental group and the test solution of the control group by using HPLC.

HPLC detection conditions: a C18 reverse direction analytical column (Eclipse plus C18, 4.6mm × 100mm, dp ═ 35 μm) was used with a suitable guard column and uv detector, using acetonitrile: water (50: 50) as mobile phase, flow rate of 1mL/min, 10min, injection volume of 10 μ L, retention time: after 7.28min, the content of malathion is detected. In HPLC analysis, taking malathion (CDCT-C14710000) as a standard substance, qualitatively analyzing the malathion according to the retention time of the standard substance and quantitatively analyzing the malathion by adopting a standard curve method (an external standard method), and calculating the degradation rate of the malathion according to the following formula:

the malathion degradation rate (the content of the malathion before degradation-the content of the malathion after degradation)/the content of the malathion before degradation x 100%

After culturing in an inorganic salt culture medium for 12 hours, the HPLC detection result is shown in figure 8, which shows that characteristic peaks appear when the retention time of malathion in a control group is 7.28min, characteristic peaks are detected when an experimental group is 7.281min, the degradation rate of the malathion in the experimental group is 65.2% according to the peak area, and the calculation formula is as follows:

the malathion degradation rate (the content of the malathion before the reaction-the content of the malathion after the reaction)/the content of the malathion before the reaction multiplied by 100 percent

After culturing in an inorganic salt culture medium for 18h, the HPLC detection result is shown in figure 9, which shows that characteristic peaks appear in the malathion in the control group and the experimental group when the retention time is 7.286min, and the degradation rate of the malathion in the experimental group is 91.1% according to the peak area calculation.

After the strain is cultured in an inorganic salt culture medium for 24 hours, the HPLC detection result is shown in figure 10, which shows that characteristic peaks appear when the retention time is 7.274min in malathion in a control group, characteristic peaks are detected when the retention time is 7.28min in an experimental group, and the degradation rate of the malathion in the experimental group is 96.8% according to peak area calculation.

After the strain is cultured in an inorganic salt culture medium for 30 hours, the HPLC detection result is shown in figure 11, which shows that characteristic peaks appear when the retention time of malathion in a control group is 7.271min, characteristic peaks are detected when an experimental group is retained for 7.28min, and the degradation rate of the malathion in the experimental group is 98.5% according to peak area calculation.

After culturing in the inorganic salt culture medium for 40h, the HPLC detection result is shown in FIG. 12, which shows that malathion in the control group has a characteristic peak at the retention time of 7.282min, and the characteristic peak is not detected in the experimental group at the retention time of 7.28min, i.e. the malathion degradation rate of the experimental group is 100% after culturing for 40 h.

The change dynamics of the degradation rate of malathion when the strain R2059-CB1-ML is cultured in a mineral salt medium is shown in figure 13.

In conclusion, the pseudomarine bacteria R2059-CB1-ML can be used as a bacterial strain living in seawater to degrade malathion and can be used for marine pollution treatment.

The present invention has been described in detail above. It will be apparent to those skilled in the art that the invention can be practiced in a wide range of equivalent parameters, concentrations, and conditions without departing from the spirit and scope of the invention and without undue experimentation. While the invention has been described with reference to specific embodiments, it will be appreciated that the invention can be further modified. In general, this application is intended to cover any variations, uses, or adaptations of the invention following, in general, the principles of the invention and including such departures from the present disclosure as come within known or customary practice within the art to which the invention pertains. The use of some of the essential features is possible within the scope of the claims attached below.

Sequence listing

<110> institute of microbiology of Chinese academy of sciences

<120> pseudomarine bacterial strain with obvious degradation effect on malathion and application thereof

<130> GNCSY213344

<160> 1

<170> SIPOSequenceListing 1.0

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<211> 1507

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<213> Pseudomarine genus bacterium (pseudoiomarina sp.)

<400> 1

agagtttgat catggctcag attgaacgct ggcggcaggc ctaacacatg caagtcgagc 60

ggtaacagag agaagcttgc ttctttgctg acgagcggcg gacgggtgag taatacttgg 120

gaacttgcct ttaggagggg gacaaccacg ggaaactgtg gctaataccg cataatgtct 180

acggaccaaa gcaggggatc ttcggacctt gtacctaaag atgggcccaa gcgagattag 240

ctagttggtg gggtaaaggc tcaccaaggc gacgatctct agctgttctg agaggatgat 300

cagccacact gggactgaga cacggcccag actcctacgg gaggcagcag tggggaatat 360

tgcacaatgg gggaaaccct gatgcagcca tgccgcgtgt gtgaagaagg ccttcgggtt 420

gtaaagcact ttcagtggtg aggaaggcca tacacttaat acgtgtgtgg attgacgtta 480

accacagaag aagcaccggc taactccgtg ccagcagccg cggtaatacg gagggtgcga 540

gcgttaatcg gaattactgg gcgtaaagcg tacgtaggcg gcttgttaag caagatgtga 600

aagccccggg ctcaacctgg gaatggcatt ttgaactggc aagctcgagt tctgaagagg 660

gtggtagaat ttcctgtgta gcggtgaaat gcgtagatat aggaaggaat accggtggcg 720

aaggcggcca cctggtcaga aactgacgct gaggtacgaa agcgtgggga gcaaacagga 780

ttagataccc tggtagtcca cgccgtaaac gatgtcaact agttgttcgt cttataaaca 840

aggtgagtaa cgcagctaac gcactaagtt gaccgcctgg ggagtacggc cgcaaggtta 900

aaactcaaat gaattgacgg gggcccgcac aagcggtgga gcatgtggtt taattcgatg 960

caacgcgaag aaccttacca tcccttgaca tccagtgaac tttctagaga tagattggtg 1020

ccttcgggaa cactgagaca ggtgctgcat ggctgtcgtc agctcgtgtt gtgagatgtt 1080

gggttaagtc ccgcaacgag cgcaaccctt atcctcagtt gccagcacat aatggtggga 1140

actctgtgga gactgccggt gataaaccgg aggaaggtgg ggacgacgtc aagtcatcat 1200

ggcccttacg ggatgggcta cacacgtgct acaatggcgc gtacaatggg aagcaagcta 1260

gcgatagtaa gcggatctca aaaagcgcgt cgtagtccgg atcggagtct gcaactcgac 1320

tccgtgaagt cggaatcgct agtaatcgtg gatcagaatg ccacggtgaa tacgttcccg 1380

ggccttgtac acaccgcccg tcacaccatg ggagtgggct gcaccagaag tggatagttt 1440

aaccttcggg agaacgttca ccacggtgtg gttcatgact ggggtgaagt cgtaacaagg 1500

tagccgt 1507

Claims

1. The pseudo-marine bacteria are characterized in that: the pseudo-marine bacteria are pseudo-marine bacteria (pseudomarine sp), the strain number of the pseudo-marine bacteria is R2059-CB1-ML, and the registration number of the pseudo-marine bacteria in the common microorganism center of China Committee for culture Collection of microorganisms is CGMCC No. 23970.

2. Use of the pseudomarine bacterium or/and the metabolite of the pseudomarine bacterium according to claim 1 for any of the following:

3. A product characterized by: comprises the metabolite of the pseudomarine bacterium R2059-CB1-ML or/and the pseudomarine bacterium R2059-CB1-ML as claimed in claim 1.

4. The product of claim 3, wherein: is any one of the following products:

v1, products for degrading organic phosphorus;

v2, a product for degrading malathion;

v3, a product for improving marine environment;

v4, processing products polluted by seawater.

5. Use of the product of claim 3 or 4 in any of the following applications:

x1, the use of said product for degrading organic phosphorus;

x2, the use of said product in degrading malathion;

x3, use of said product in modifying the marine environment;

x4, use of said product in the treatment of seawater pollution.

6. The culture of pseudomarine bacteria of claim 1, characterized in that: the culture has at least one function of the following W1-W4:

w1, degrading organic phosphorus;

w2, degrading malathion;

w3, modified marine environment;

w4, treating seawater pollution.

7. The method for culturing the pseudomarine bacterium R2059-CB1-ML according to claim 1, wherein: comprising the step of culturing said pseudomarine bacteria in a culture medium.

8. A process for preparing the product of any of claims 3 or 4, characterized in that: comprises the step of obtaining the product by taking the pseudomarine bacteria R2059-CB1-ML and/or the metabolite of the pseudomarine bacteria R2059-CB1-ML as the components of the product.

9. The method of claim 8, wherein: the product is a liquid microbial inoculum or a solid microbial inoculum.