CN114180184A - Cell cryopreserving frame - Google Patents
Cell cryopreserving frame Download PDFInfo
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- CN114180184A CN114180184A CN202111359346.7A CN202111359346A CN114180184A CN 114180184 A CN114180184 A CN 114180184A CN 202111359346 A CN202111359346 A CN 202111359346A CN 114180184 A CN114180184 A CN 114180184A
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- cell test
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- 230000000694 effects Effects 0.000 claims abstract description 11
- 238000005138 cryopreservation Methods 0.000 claims abstract description 8
- 238000007710 freezing Methods 0.000 claims description 22
- 230000008014 freezing Effects 0.000 claims description 22
- 239000004065 semiconductor Substances 0.000 claims description 14
- 238000005057 refrigeration Methods 0.000 claims description 12
- 230000002633 protecting effect Effects 0.000 claims description 7
- 239000011241 protective layer Substances 0.000 claims description 7
- 230000003139 buffering effect Effects 0.000 claims description 6
- 230000007246 mechanism Effects 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 6
- 230000035939 shock Effects 0.000 claims description 5
- 239000010410 layer Substances 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 239000011435 rock Substances 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 229910001220 stainless steel Inorganic materials 0.000 claims description 3
- 239000010935 stainless steel Substances 0.000 claims description 3
- 230000008878 coupling Effects 0.000 claims 1
- 238000010168 coupling process Methods 0.000 claims 1
- 238000005859 coupling reaction Methods 0.000 claims 1
- 238000013016 damping Methods 0.000 abstract description 3
- 230000000249 desinfective effect Effects 0.000 abstract description 2
- 230000002093 peripheral effect Effects 0.000 abstract description 2
- 230000001954 sterilising effect Effects 0.000 abstract 1
- 230000001603 reducing effect Effects 0.000 description 4
- 238000004321 preservation Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
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- 239000007788 liquid Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D7/00—Containers having bodies formed by interconnecting or uniting two or more rigid, or substantially rigid, components made wholly or mainly of metal
- B65D7/12—Containers having bodies formed by interconnecting or uniting two or more rigid, or substantially rigid, components made wholly or mainly of metal characterised by wall construction or by connections between walls
- B65D7/14—Containers having bodies formed by interconnecting or uniting two or more rigid, or substantially rigid, components made wholly or mainly of metal characterised by wall construction or by connections between walls of skeleton or like apertured construction, e.g. baskets or carriers formed of wire mesh, of interconnected bands, bars, or rods, or of perforated sheet metal
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D11/00—Containers having bodies formed by interconnecting or uniting two or more rigid, or substantially rigid, components made wholly or mainly of plastics material
- B65D11/14—Containers having bodies formed by interconnecting or uniting two or more rigid, or substantially rigid, components made wholly or mainly of plastics material of skeleton or apertured construction, e.g. baskets or carriers made up of interconnected spaced bands, rods, or strips
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D25/00—Details of other kinds or types of rigid or semi-rigid containers
- B65D25/02—Internal fittings
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D25/00—Details of other kinds or types of rigid or semi-rigid containers
- B65D25/02—Internal fittings
- B65D25/10—Devices to locate articles in containers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D53/00—Sealing or packing elements; Sealings formed by liquid or plastics material
- B65D53/02—Collars or rings
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/02—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents specially adapted to protect contents from mechanical damage
- B65D81/05—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents specially adapted to protect contents from mechanical damage maintaining contents at spaced relation from package walls, or from other contents
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/18—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
A cell cryopreservation frame comprises a storage tank, rectangular stand columns and an electric appliance box, wherein a plurality of fixed cylinders are arranged on the peripheral surface of the middle part of the storage tank at equal angles, springs are arranged in the fixed cylinders, the upper ends and the lower ends of the springs are connected with a top plate, the upper ends of the springs are connected with a first buffer rod through the top plate, the upper ends of the first buffer rods penetrate through the fixed cylinders to be commonly supported and connected with an upper anti-collision ring, the lower ends of the springs are connected with a second buffer rod through the top plate, the lower ends of the second buffer rods penetrate through the fixed cylinders to be commonly connected with a lower anti-collision ring, and the upper end of the storage tank is in threaded connection with a shell cover; the cell test tube is firmly fixed and prevented from shaking, the cell test tube is convenient to take and place, the cell test tube can be directly placed for use without troublesome transfer, the cell test tube is environment-friendly and energy-saving, is convenient to carry under the power supply of a battery, has good anti-collision performance and good damping and protection effects, can well protect the storage tank and the cell test tube inside the storage tank, reduces property loss, and is safe and sanitary due to the fact that the ultraviolet lamp is used for regularly sterilizing and disinfecting.
Description
Technical Field
The invention relates to the technical field of cell storage equipment, in particular to a cell freezing frame.
Background
The cell freezing is one of the main methods for cell preservation, and the freezing technology is utilized to place the cells in liquid nitrogen at-196 ℃ for low-temperature preservation, so that the cells can be temporarily separated from the growth state to preserve the cell characteristics, and the cell preservation effect is achieved.
At present, the existing cell freezing frame is large in size, inconvenient to place, complex in structure, complex in cell freezing taking and placing, time and labor are wasted due to the fact that a cell freezing test tube needs to be additionally transferred to a test tube frame after being taken out, meanwhile, the existing cell freezing frame is poor in protection performance, damage to equipment and the cell freezing test tube is caused very easily in accidental collision, the shock resistance and buffering performance of the existing cell freezing frame are poor, and the cell freezing test tube is prone to collide with the cell freezing frame to cause cell outflow and great loss in transportation.
Disclosure of Invention
Objects of the invention
In order to solve the technical problems in the background art, the invention provides a cell freezing rack, a cell test tube is firmly fixed and prevented from shaking, the cell freezing rack is convenient to take and place, the cell test tube can be directly placed for use without trouble transfer, the cell freezing rack is environment-friendly and energy-saving, battery power supply is convenient to carry, the anti-collision performance is good, the damping protection effect is good, a storage tank and the cell test tube inside the storage tank can be well protected, property loss is reduced, and an ultraviolet lamp is used for regular sterilization and disinfection, and is safe and sanitary.
(II) technical scheme
In order to solve the problems, the invention provides a cell cryopreservation rack which comprises a storage tank, rectangular stand columns and an electric appliance box, wherein a plurality of fixed cylinders are arranged on the peripheral surface of the middle part of the storage tank at equal angles, springs are arranged in the fixed cylinders, top plates are connected with the upper ends and the lower ends of the springs respectively, the upper ends of the springs are connected with first buffer rods through the top plates, the upper ends of the first buffer rods penetrate through the fixed cylinders to be commonly supported and connected with upper anti-collision rings, the lower ends of the springs are connected with second buffer rods through the top plates, the lower ends of the second buffer rods penetrate through the fixed cylinders to be commonly connected with lower anti-collision rings, a shell cover is in threaded connection with the upper end of the storage tank, a handle is arranged in the middle of the upper end of the shell cover, the rectangular stand columns are arranged on the left side in the storage tank, rectangular through grooves are arranged in the middle of the right ends of the rectangular stand columns, guide slide rods are symmetrically arranged on the front and the back sides of the rectangular through grooves, and the lower parts of the guide slide rods are commonly and slidably connected with vertical slide plates, a lifting power module is arranged inside the rectangular upright post;
perpendicular slide right-hand member middle part is provided with the lifter plate, the pull rod is connected through wabbler mechanism in the middle part of the lifter plate upper end, pull rod lower part cover is equipped with down the plectane, the plectane upper end is provided with a plurality of recesses, pull rod upper portion cover is equipped with the plectane, it is provided with a plurality of through-holes to go up the plectane upper end, it has the cell test tube all to settle in the through-hole, cell test tube lower extreme extends to in the recess respectively, pull rod upper end middle part is provided with the pull ring, the inside upper right side of holding vessel is provided with the purple light lamp, the inside bottom right side of holding vessel is provided with the electrical apparatus box, the embedded semiconductor refrigeration piece that is provided with in electrical apparatus box upper end left side, the embedded temperature sensor that is provided with in electrical apparatus box left end, the inside bottom of electrical apparatus box is provided with the battery, the inside top of electrical apparatus box is provided with the singlechip.
Preferably, equidistant cover is equipped with a plurality of antiskid covers on the crashproof ring down, and the number of antiskid cover is four at least, and the cap lower extreme just is provided with the sealing washer with the junction of holding vessel is embedded.
Preferably, the materials of the first buffer rod, the second buffer rod, the upper anti-collision ring and the lower anti-collision ring are all stainless steel.
Preferably, the lifting power module comprises a servo motor, a screw rod and a ball sliding sleeve, the servo motor is arranged at the bottom end inside the rectangular stand column, a motor shaft of the servo motor is connected with the screw rod through a coupler, the upper end of the screw rod is connected with the rectangular stand column in a rotating mode, the lower portion of the screw rod is in threaded connection with the ball sliding sleeve, and the right portion of the ball sliding sleeve penetrates through the rectangular through groove through a connecting block to be connected with the vertical sliding plate.
Preferably, the swing mechanism comprises an elastic rubber block, a threaded rod and a threaded cap, the threaded rod is arranged in the middle of the upper end of the elastic rubber block, the threaded cap is connected to the upper portion of the threaded rod in a threaded mode, and the upper end of the threaded cap is connected with the pull rod.
Preferably, the material of support inoxidizing coating is the sponge, and lower plectane lower extreme edge is the equidistance and is provided with a plurality of supporting legs, and the supporting leg lower extreme all is provided with the rubber spheroid.
Preferably, the type of the semiconductor refrigerating piece is TEC1-04902, the type of the temperature sensor is TP21TP100, the type of the single chip microcomputer is ARM Cortex-A9, and the single chip microcomputer is electrically connected with the servo motor, the semiconductor refrigerating piece, the temperature sensor, the switch group, the battery and the ultraviolet lamp.
Preferably, the working method of the cell cryopreservation rack comprises the following specific steps:
when the cell test tube is used, firstly, the cell test tube penetrates through a through hole at the upper end of the upper circular plate, and the bottom of the cell test tube is positioned in a groove for supporting the protective layer, so that the cell test tube is firmly fixed and prevented from shaking;
step two, opening the shell cover, starting a servo motor through a switch group to drive a screw rod to rotate, driving a vertical sliding plate and a lifting plate to move upwards along a guide sliding rod through a ball sliding sleeve, aligning a threaded cap with a threaded rod, then rotationally fixing, quickly and conveniently installing, and similarly, after the servo motor reversely rotates to drive a cell test tube to enter a storage tank, covering the shell cover;
step three, the temperature sensor detects real-time temperature data and transmits the real-time temperature data to the single chip microcomputer, when the temperature is higher than a threshold value set in the single chip microcomputer, refrigeration is carried out through a semiconductor refrigeration piece, and the battery supplies power and is convenient to carry;
step four, when the equipment is collided or falls down carelessly, the upper anti-collision ring or the lower anti-collision ring transmits the impact force contacting the ground to the top plate through the first buffer rod or the second buffer rod, and the top plate extrudes the spring to obtain a buffer space, so that the storage tank and the cell test tube inside the storage tank are well protected;
step five, when personnel or vehicle rock in the transportation and jolt, under the effect of elastic rubber piece, the pull rod carries out small amplitude swing buffering to carry on spacingly through the supporting leg, avoid causing the separation from or damage the inside cell test tube of device, the cell flows out and causes the loss, has further promoted shock attenuation protective effect.
The technical scheme of the invention has the following beneficial technical effects: the cell test tube of the device is firmly fixed and avoids shaking, the taking and the placing are convenient, the cell test tube can be directly placed when being taken out for use, the test tube rack does not need to be additionally transferred, the refrigeration and the temperature regulation can be automatically carried out, the energy consumption is saved, and the battery is convenient to carry, when the equipment is collided or falls down by accident, the impact force contacting with the ground is transmitted to the top plate by the upper anti-collision ring or the lower anti-collision ring through the first buffer rod or the second buffer rod, the buffer space is obtained by the top plate extruding the spring, thereby protecting the storage tank and the cell test tube inside the storage tank well, reducing property loss, and reducing the possibility that personnel or vehicles shake and bump in the transportation process, under the effect of elastic rubber piece, the pull rod carries out the buffering of small amplitude swing to carry on spacingly through the supporting leg, avoid causing to break away from or damage the inside cell test tube of device, the cell outflow causes the loss, has further promoted shock attenuation protecting effect.
Drawings
FIG. 1 is a cross-sectional view of a cell cryopreservation rack according to the present invention.
Fig. 2 is an isometric view of a storage tank according to the present invention.
FIG. 3 is an enlarged view of A in the cell freezing rack according to the present invention.
FIG. 4 is a top view of a cell cryopreservation rack according to the present invention.
Reference numerals: 1. a storage tank; 2. a fixed cylinder; 3. a spring; 4. a top plate; 5. a first buffer rod; 6. a second buffer rod; 7. a lower anti-collision ring; 8. an anti-slip sleeve; 9. a shell cover; 10. a handle; 11. a rectangular column; 12. a rectangular through groove; 13. a guide slide bar; 14. a vertical slide plate; 15. a servo motor; 16. a screw rod; 17. a ball sliding sleeve; 18. a lifting plate; 19. an elastic rubber block; 20. a threaded rod; 21. a threaded cap; 22. a pull rod; 23. a lower circular plate; 24. supporting legs; 25. supporting the protective layer; 26. an upper circular plate; 27. a cell tube; 28. a pull ring; 29. an electrical box; 30. a semiconductor refrigeration sheet; 31. a temperature sensor; 32. a switch group; 33. a battery; 34. a single chip microcomputer; 35. ultraviolet lamp.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail with reference to the accompanying drawings in conjunction with the following detailed description. It should be understood that the description is intended to be exemplary only, and is not intended to limit the scope of the present invention. Moreover, in the following description, descriptions of well-known structures and techniques are omitted so as to not unnecessarily obscure the concepts of the present invention.
As shown in FIGS. 1-4, the cell cryopreservation rack provided by the invention comprises a storage tank 1, a rectangular upright post 11 and an electrical appliance box 29, wherein a plurality of fixed cylinders 2 are arranged on the circumferential surface of the middle part of the storage tank 1 at equal angles, springs 3 are arranged in the fixed cylinders 2, the upper ends and the lower ends of the springs 3 are connected with a top plate 4, the upper ends of the springs 3 are connected with first buffer rods 5 through the top plate 4, the upper ends of the first buffer rods 5 penetrate through the fixed cylinders 2 to be commonly supported and connected with upper anti-collision rings, the lower ends of the springs 3 are connected with second buffer rods 6 through the top plate 4, the lower ends of the second buffer rods 6 penetrate through the fixed cylinders 2 to be commonly connected with lower anti-collision rings 7, the materials of the first buffer rods 5, the second buffer rods 6, the upper anti-collision rings and the lower anti-collision rings 7 are all made of stainless steel, the upper end of the storage tank 1 is in threaded connection with a shell cover 9, the lower anti-collision rings 7 are sleeved with a plurality of anti-collision sleeves 8 at equal intervals, the number of the anti-skid sleeves 8 is at least four;
a sealing ring is embedded in the joint of the lower end of the shell cover 9 and the storage tank 1, a handle 10 is arranged in the middle of the upper end of the shell cover 9, a rectangular upright post 11 is arranged on the left side inside the storage tank 1, a rectangular through groove 12 is arranged in the middle of the right end of the rectangular upright post 11, guide slide bars 13 are symmetrically arranged at the right end of the rectangular upright post 11 and positioned at the front side and the rear side of the rectangular through groove 12, the lower parts of the guide slide bars 13 are jointly and slidably connected with a vertical slide plate 14, a lifting power module is arranged inside the rectangular upright post 11 and comprises a servo motor 15, the servo motor 15 is arranged at the bottom end inside the rectangular upright post 11, a motor shaft of the servo motor 15 is connected with the screw rod 16 through a coupler, the upper end of the screw rod 16 is rotatably connected with the rectangular upright post 11, the lower part of the screw rod 16 is in threaded connection with the ball sliding sleeve 17, and the right part of the ball sliding sleeve 17 penetrates through the rectangular through groove 12 through a connecting block and is connected with the vertical sliding plate 14;
the middle part of the right end of the vertical sliding plate 14 is provided with a lifting plate 18, the middle part of the upper end of the lifting plate 18 is connected with a pull rod 22 through a swinging mechanism, the swinging mechanism comprises an elastic rubber block 19, the cell test tube comprises a threaded rod 20 and a threaded cap 21, the threaded rod 20 is arranged in the middle of the upper end of an elastic rubber block 19, the threaded cap 21 is connected to the upper portion of the threaded rod 20 in a threaded mode, a pull rod 22 is connected to the upper end of the threaded cap 21, a lower circular plate 23 is sleeved on the lower portion of the pull rod 22, a supporting and protecting layer 25 is arranged at the upper end of the lower circular plate 23, a plurality of grooves are formed in the upper end of the supporting and protecting layer 25, the supporting and protecting layer 25 is made of sponge, a plurality of supporting legs 24 are arranged on the edge of the lower end of the lower circular plate 23 at equal angles, rubber balls are arranged at the lower ends of the supporting legs 24, an upper circular plate 26 is sleeved on the upper portion of the pull rod 22, a plurality of through holes are formed in the upper end of the upper circular plate 26, cell test tubes 27 are arranged in the through holes, the lower ends of the cell test tubes 27 extend into the grooves respectively, and a pull ring 28 is arranged in the middle of the upper end of the pull rod 22;
the ultraviolet lamp 35 is arranged on the upper right side inside the storage tank 1, the electric box 29 is arranged on the right side of the bottom inside the storage tank 1, the semiconductor refrigeration piece 30 is embedded in the left side of the upper end of the electric box 29, the temperature sensor 31 is embedded in the right side of the upper end of the electric box 29, the switch group 32 is embedded in the left end of the electric box 29, the battery 33 is arranged at the bottom inside the electric box 29, the single chip microcomputer 34 is arranged at the top inside the electric box 29, the semiconductor refrigeration piece 30 is TEC1-04902, the temperature sensor 31 is TP21TP100, the single chip microcomputer 34 is ARM Cortex-A9, and the single chip microcomputer 34 is electrically connected with the servo motor 15, the semiconductor refrigeration piece 30, the temperature sensor 31, the switch group 32, the battery 33 and the ultraviolet lamp 35;
the working method of the cell cryopreservation rack comprises the following specific steps:
when the cell test tube is used, firstly, the cell test tube 27 penetrates through a through hole at the upper end of the upper circular plate 26, and the bottom of the cell test tube is positioned in a groove of the supporting protective layer 25, so that the cell test tube is firmly fixed and is prevented from shaking;
step two, opening the shell cover 9, starting the servo motor 15 through the switch group 32 to drive the screw rod 16 to rotate, so as to drive the vertical sliding plate 14 and the lifting plate 18 to move upwards along the guide sliding rod 13 through the ball sliding sleeve 17, aligning the threaded cap 21 with the threaded rod 20, then rotationally fixing, and quickly and conveniently installing, similarly, after the servo motor 15 reversely rotates to drive the cell test tube 27 to enter the storage tank 1, covering the shell cover 9;
step three, the temperature sensor 31 detects real-time temperature data and transmits the real-time temperature data to the single chip microcomputer 34, when the temperature is higher than a threshold value set in the single chip microcomputer 34, refrigeration is carried out through the semiconductor refrigeration piece 30, and the battery 33 supplies power so as to be convenient to carry;
step four, when the equipment is collided or falls down carelessly, the upper anti-collision ring or the lower anti-collision ring 7 transmits the impact force contacting the ground to the top plate 4 through the first buffer rod 5 or the second buffer rod 6, and the spring 3 is extruded through the top plate 4 to obtain a buffer space, so that the storage tank 1 and the cell test tube 27 in the storage tank are well protected;
step five, when personnel or vehicle rock in the transportation and jolt, under the effect of elastic rubber piece 19, pull rod 22 carries out the buffering of small amplitude swing to it is spacing through supporting leg 24, avoid causing the separation from or damage the inside cell test tube 27 of device, the cell outflow causes the loss, has further promoted shock attenuation protection effect.
The working principle of the invention is as follows: when the cell test tube holder is in use, firstly, the cell test tube 27 is fixed in the through hole at the upper end of the upper circular plate 26, the bottom of the cell test tube is positioned in the groove of the supporting protective layer 25, the cell test tube holder is firmly fixed and can be prevented from shaking, the cell test tube holder can be conveniently taken and placed by matching with the supporting leg 24, the cell test tube 27 can be conveniently taken out for use without additional transfer and placement, the shell cover 9 is opened, the servo motor 15 is started by the switch group 32 to drive the lead screw 16 to rotate, so that the vertical sliding plate 14 and the lifting plate 18 are driven to move upwards along the guide sliding rod 13 by the ball sliding sleeve 17, the threaded cap 21 is rotationally fixed after being aligned with the threaded rod 20, the disassembly and assembly and the locking are quick and convenient, after the cell test tube 27 is driven to enter the storage tank 1 by the reverse rotation of the servo motor 15, the shell cover 9 is covered, the temperature sensor 31 detects real-time temperature data and transmits the data to the singlechip 34, when the temperature is higher than the threshold value set in the singlechip 34, the semiconductor refrigerating sheet 30 is used for refrigerating, so that energy consumption is saved, and the battery 33 is used for supplying power and is convenient to carry;
when the apparatus is collided or dropped carelessly, the upper or lower impact ring 7 transmits the impact force contacting the ground to the top plate 4 through the first buffer rod 5 or the second buffer rod 6, the spring 3 is compressed by the top plate 4 to obtain a buffer space, thereby protecting the storage tank 1 and the cell test tube 27 inside the storage tank well, reducing property loss, and reducing the possibility that personnel or vehicles shake and bump during transportation, under the action of the elastic rubber block 19, the pull rod 22 performs small-amplitude swing buffering and is limited by the supporting legs 24, so that the cell test tube 27 in the device is prevented from being separated or damaged, the cell flows out to cause loss, the damping protection effect is further improved, and the device has small volume, through lower crashproof ring 7 lay conveniently, and through anti-skidding 8 stable antiskid of cover, regularly start purple light lamp 35 disinfection of disinfecting, receive the pollution when avoiding the cell to deposit.
It is to be understood that the above-described embodiments of the present invention are merely illustrative of or explaining the principles of the invention and are not to be construed as limiting the invention. Therefore, any modification, equivalent replacement, improvement and the like made without departing from the spirit and scope of the present invention should be included in the protection scope of the present invention. Further, it is intended that the appended claims cover all such variations and modifications as fall within the scope and boundaries of the appended claims or the equivalents of such scope and boundaries.
Claims (8)
1. A cell freezing frame is characterized by comprising a storage tank (1), a rectangular upright post (11) and an electric appliance box (29); the storage tank is characterized in that a plurality of fixed cylinders (2) are arranged on the circumferential surface of the middle part of the storage tank (1) at equal angles, springs (3) are arranged in the fixed cylinders (2), the upper ends and the lower ends of the springs (3) are connected with a top plate (4), the upper ends of the springs (3) are connected with first buffer rods (5) through the top plate (4), the upper ends of the first buffer rods (5) penetrate through the fixed cylinders (2) to be jointly supported and connected with upper anti-collision rings, the lower ends of the springs (3) are connected with second buffer rods (6) through the top plate (4), the lower ends of the second buffer rods (6) penetrate through the fixed cylinders (2) to be jointly connected with lower anti-collision rings (7), a shell cover (9) is in threaded connection with the upper end of the storage tank (1), a handle (10) is arranged in the middle part of the upper end of the shell cover (9), a rectangular upright column (11) is arranged on the left side in the storage tank (1), a rectangular through groove (12) is arranged in the middle part of the right end of the rectangular upright column (11), guide slide bars (13) are symmetrically arranged at the right end of the rectangular upright post (11) and positioned at the front side and the rear side of the rectangular through groove (12), the lower parts of the guide slide bars (13) are jointly connected with a vertical sliding plate (14) in a sliding manner, and a lifting power module is arranged in the rectangular upright post (11);
a lifting plate (18) is arranged in the middle of the right end of a vertical sliding plate (14), the middle of the upper end of the lifting plate (18) is connected with a pull rod (22) through a swing mechanism, a lower circular plate (23) is sleeved on the lower portion of the pull rod (22), a supporting protective layer (25) is arranged at the upper end of the lower circular plate (23), a plurality of grooves are formed in the upper end of the supporting protective layer (25), an upper circular plate (26) is sleeved on the upper portion of the pull rod (22), a plurality of through holes are formed in the upper end of the upper circular plate (26), cell test tubes (27) are arranged in the through holes, the lower ends of the cell test tubes (27) extend into the grooves respectively, a pull ring (28) is arranged in the middle of the upper end of the pull rod (22), a purple light lamp (35) is arranged on the upper right side inside the storage tank (1), an electric appliance box (29) is arranged on the right side of the bottom inside of the storage tank (1), a semiconductor refrigerating sheet (30) is arranged on the left side of the upper end of the electric appliance box (29), a temperature sensor (31) is arranged in an embedded manner, a switch group (32) is embedded in the left end of the electric appliance box (29), a battery (33) is arranged at the bottom end inside the electric appliance box (29), and a single chip microcomputer (34) is arranged at the top end inside the electric appliance box (29).
2. The cell freezing rack according to claim 1, wherein the lower anti-collision ring (7) is provided with a plurality of anti-slip sleeves (8) at equal intervals, the number of the anti-slip sleeves (8) is at least four, and a sealing ring is embedded in the lower end of the shell cover (9) and the connection part of the shell cover and the storage tank (1).
3. The cell freezing rack according to claim 1, wherein the materials of the first buffer rod (5), the second buffer rod (6), the upper anti-collision ring and the lower anti-collision ring (7) are stainless steel.
4. The cell cryopreservation rack according to claim 1, wherein the lifting power module comprises a servo motor (15), a screw rod (16) and a ball sliding sleeve (17), the servo motor (15) is arranged at the bottom end inside the rectangular upright post (11), a motor shaft of the servo motor (15) is connected with the screw rod (16) through a coupling, the upper end of the screw rod (16) is rotatably connected with the rectangular upright post (11), the ball sliding sleeve (17) is in threaded connection with the lower part of the screw rod (16), and the right part of the ball sliding sleeve (17) penetrates through the rectangular through groove (12) through a connecting block to be connected with the vertical sliding plate (14).
5. The cell freezing rack according to claim 1, wherein the swinging mechanism comprises an elastic rubber block (19), a threaded rod (20) and a threaded cap (21), the threaded rod (20) is arranged in the middle of the upper end of the elastic rubber block (19), the threaded cap (21) is connected to the upper portion of the threaded rod (20) in a threaded manner, and the pull rod (22) is connected to the upper end of the threaded cap (21).
6. The cell freezing rack according to claim 1, wherein the supporting and protecting layer (25) is made of sponge, the lower edge of the lower circular plate (23) is provided with a plurality of supporting legs (24) at equal angles, and the lower ends of the supporting legs (24) are provided with rubber spheres.
7. The cell freezing rack according to claim 1, wherein the semiconductor refrigerating plate (30) is TEC1-04902, the temperature sensor (31) is TP21TP100, the single chip microcomputer (34) is ARM Cortex-A9, and the single chip microcomputer (34) is electrically connected with the servo motor (15), the semiconductor refrigerating plate (30), the temperature sensor (31), the switch group (32), the battery (33) and the ultraviolet lamp (35).
8. The working method of the cell freezing rack according to any one of claims 1-7, comprising the following steps:
when the cell test tube is used, firstly, the cell test tube (27) penetrates through a through hole at the upper end of the upper circular plate (26), and the bottom of the cell test tube is positioned in a groove of the supporting protective layer (25), so that the cell test tube is firmly fixed and is prevented from shaking;
step two, opening the shell cover (9), starting the servo motor (15) through the switch group (32) to drive the screw rod (16) to rotate, driving the vertical sliding plate (14) and the lifting plate (18) to move upwards along the guide sliding rod (13) through the ball sliding sleeve (17), rotating and fixing after aligning the threaded cap (21) with the threaded rod (20), and quickly and conveniently installing, and similarly, after the cell test tube (27) is driven by the reverse rotation of the servo motor (15) to enter the storage tank (1), covering the shell cover (9);
step three, the temperature sensor (31) detects real-time temperature data and transmits the real-time temperature data to the single chip microcomputer (34), when the temperature is higher than a threshold value set in the single chip microcomputer (34), refrigeration is carried out through the semiconductor refrigeration piece (30), and the battery (33) supplies power and is convenient to carry;
step four, when the equipment is collided or falls down carelessly, the upper anti-collision ring or the lower anti-collision ring (7) transmits the impact force contacting the ground to the top plate (4) through the first buffer rod (5) or the second buffer rod (6), and the top plate (4) extrudes the spring (3) to obtain a buffer space, so that the storage tank (1) and the cell test tube (27) in the storage tank are well protected;
step five, when personnel or vehicle rock the jolt in the transportation, under the effect of elastic rubber piece (19), pull rod (22) carry out the buffering of small amplitude swing to carry on spacingly through supporting leg (24), avoid causing and break away from or damage the inside cell test tube (27) of device, the cell flows out and causes the loss, has further promoted shock attenuation protection effect.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111359346.7A CN114180184A (en) | 2021-11-17 | 2021-11-17 | Cell cryopreserving frame |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111359346.7A CN114180184A (en) | 2021-11-17 | 2021-11-17 | Cell cryopreserving frame |
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| CN114180184A true CN114180184A (en) | 2022-03-15 |
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| CN202111359346.7A Pending CN114180184A (en) | 2021-11-17 | 2021-11-17 | Cell cryopreserving frame |
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| CN115072142A (en) * | 2022-07-27 | 2022-09-20 | 山东第一医科大学附属省立医院(山东省立医院) | Instant aseptic preservation and transfer equipment for resected lung tumor |
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| CN115633677A (en) * | 2022-08-29 | 2023-01-24 | 锦州医科大学 | Novel animal semen cryopreservation device and cryopreservation method |
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