CN114177649B - Salting-out device for separating and purifying animal serum protein - Google Patents
Salting-out device for separating and purifying animal serum protein Download PDFInfo
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- CN114177649B CN114177649B CN202111297374.0A CN202111297374A CN114177649B CN 114177649 B CN114177649 B CN 114177649B CN 202111297374 A CN202111297374 A CN 202111297374A CN 114177649 B CN114177649 B CN 114177649B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D12/00—Displacing liquid, e.g. from wet solids or from dispersions of liquids or from solids in liquids, by means of another liquid
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
- C07K1/303—Extraction; Separation; Purification by precipitation by salting out
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
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Abstract
The invention relates to the technical field of animal serum protein separation, in particular to a salting-out device for separating and purifying animal serum proteins, which comprises a salting-out device body, a neutral salt adjusting feeding mechanism and a discharging mechanism, wherein a neutral salt storage bin is fixedly arranged at the upper end of the inner side of the salting-out device body, a stirring rotating shaft is rotatably arranged in the neutral salt storage bin, the magnetic force of an electromagnet is adjusted, a permanent magnet on the surface of a sliding plate acts on the surface of the sliding plate to drive the sliding plate to slide on the surface of the sliding shaft so as to drive a filling rod to slide in a transfer groove, the capacity in the transfer groove is adjusted, the added neutral salt is adjusted, a piston rod is driven to move when the sliding plate moves, so that liquid in a hollow groove is extruded, the liquid enters a transition box, so that the liquid enters an adjusting quantity observation liquid level column, a floating ball in the adjusting quantity observation liquid level column changes, and the capacity in the current transfer groove can be known by comparing the floating ball with the scale of the adjusting quantity observation liquid level column surface.
Description
Technical Field
The invention relates to the technical field of animal serum protein separation, in particular to a salting-out device for animal serum protein separation and purification.
Background
Salting out refers to the phenomenon in which neutral salt is added to an aqueous protein solution, and protein precipitates as the salt concentration increases. Neutral salt is strong electrolyte and has high solubility, and in protein solution, on one hand, the neutral salt competes with protein for water molecules to destroy water films on the surfaces of protein colloid particles; on the other hand, the charges on the protein particles are greatly neutralized, so that the protein particles in the water are accumulated and precipitated. The obtained protein is generally not deactivated and can be redissolved under certain conditions, so the method for precipitating the protein has wide application in the work of separating, concentrating, storing and purifying the protein.
Salting out is one of the indispensable procedures of removing impurities in the process of separating and purifying animal serum proteins, however, conventionally used salting out devices cannot quantitatively and uniformly add neutral salt in the process of salting out, and protein substances after salting out are inconvenient to take out, so that the salting out device for separating and purifying animal serum proteins is required to improve the problems.
Disclosure of Invention
The invention aims to provide a salting-out device for separating and purifying animal serum proteins, which aims to solve the problems in the background technology.
In order to achieve the above purpose, the present invention provides the following technical solutions:
the utility model provides a salting out device for animal serum protein separation and purification, includes salting out device body, neutral salt regulation feed mechanism and discharge mechanism, the inboard upper end of salting out device body is fixed to be equipped with neutral salt storage bin, neutral salt storage bin inside rotation is equipped with the thumb wheel pivot, neutral salt storage bin lower extreme export and transport section of thick bamboo UNICOM setting, neutral salt storage bin lower extreme is equipped with neutral salt regulation feed mechanism, neutral salt regulation feed mechanism lower extreme exit linkage is equipped with the funnel, funnel lower extreme exit linkage is equipped with the mixing bin, mixing bin upper end is connected with the material filling mouth through the pipeline, mixing bin inside rotation is equipped with the (mixing) shaft, mixing bin lower extreme surface is equipped with the breach, mixing bin lower extreme surface is equipped with accomodates the spout, accomodate the inside slip of spout and be equipped with the closing plate and breach looks adaptation, mixing bin lower extreme is equipped with discharge mechanism, salting out device body one side surface is fixed to be equipped with the electric box, electric box inside is equipped with first driving motor, second driving motor and third driving motor, and third driving motor are connected with the feed dog, and second driving motor transmission and first driving shaft connection;
the neutral salt adjusting and feeding mechanism comprises an adjusting quantity observing liquid level column, a floating ball, a transition box, a driving rotating shaft, a connecting plate, an electromagnet, a piston rod, a filling rod, a transferring roller, a transferring groove, a hollow groove, a transferring cylinder, a hollow shaft, a sliding shaft, a permanent magnet and a sliding plate, and the discharging mechanism comprises a screw rod, a gear, a toothed bar, a toothed plate, a screw rod nut, a sealing plate, a sliding sleeve, a filtering basin, a receiving funnel and a drain pipe.
As a preferable scheme of the invention, the transfer roller is rotatably connected to the inner side of the transfer cylinder, one end of the transfer roller is in transmission connection with the third driving motor through the driving rotating shaft, and the other end of the transfer roller is rotatably connected to the inside of the transition box through the hollow shaft.
As a preferable scheme of the invention, the regulating quantity observation liquid level column, the transition box, the hollow shaft and the hollow groove are communicated, one end of the piston rod is slidably connected in the hollow groove, the other end of the piston rod is fixedly connected with the center of the sliding plate, the surface of the regulating quantity observation liquid level column is provided with scale marks, and the inside of the regulating quantity observation liquid level column is provided with a floating ball.
As a preferable scheme of the invention, the surface of the transfer roller is provided with a plurality of transfer grooves, a filling rod is slidably arranged in each transfer groove, one end of the filling rod is fixedly provided with a sliding plate, one side surface of the sliding plate is fixedly provided with a permanent magnet, and the inner side of the transfer cylinder is fixedly provided with an electromagnet corresponding to the permanent magnet.
As a preferable scheme of the invention, a plurality of sliding shafts are arranged on one end surface of the transfer roller in a circle center array, a connecting plate is fixedly arranged at one end of each sliding shaft, the connecting plate is fixedly connected with the driving rotating shaft, and the sliding plates are in sliding connection with the sliding shafts.
As a preferable scheme of the invention, the screw rod is connected with the screw rod nut through threads, and the toothed plate and the sealing plate are fixedly connected with the screw rod nut.
As a preferable scheme of the invention, the sliding sleeve is fixedly connected inside the salting-out device body, the toothed bar is slidingly connected inside the sliding sleeve, the inside of the salting-out device body is rotationally provided with the gear, the surfaces of the toothed plate and the toothed bar are fixedly provided with the meshing grooves, and the gear is in meshing connection with the meshing grooves on the surfaces of the toothed plate and the toothed bar.
As a preferable scheme of the invention, one end of the toothed bar is fixedly connected with the filtering basin, the filtering basin is arranged up and down corresponding to the notch, and the bottom of the filtering basin is provided with the filter screen.
As a preferable scheme of the invention, the lower end of the filter basin is correspondingly provided with a receiving funnel, and the surface of one side of the receiving funnel is fixedly connected with a drain pipe.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the invention, the magnetic force of the electromagnet is regulated, the permanent magnet on the surface of the sliding plate acts on the surface of the sliding shaft to drive the sliding plate to slide on the surface of the sliding shaft, so that the filling rod is driven to slide in the transfer groove, the capacity in the transfer groove is regulated, the neutral salt amount added is regulated, the sliding plate drives the piston rod to move when moving, so that the liquid in the hollow groove is extruded, the liquid enters the transition box, so that the liquid enters the regulating-amount observation liquid level column, the floating ball in the regulating-amount observation liquid level column is changed, and the capacity in the transfer groove can be known at present by comparing the floating ball with the scale of the surface of the regulating-amount observation liquid level column;
2. according to the invention, the first driving motor is started to drive the screw rod to rotate, so that the screw rod nut is driven to move, the toothed plate and the sealing plate are indirectly driven to move, the sealing plate slides in the accommodating chute to open the notch, mixed liquid is discharged into the filtering basin to be filtered, filtered water enters the receiving funnel and is discharged through the drain pipe, at the moment, the toothed plate is not meshed with the gear, when filtering is completed, the toothed plate is continuously moved, the toothed plate is in contact engagement with the gear, the toothed rod is driven to slide in the sliding sleeve by rotation, so that the filtering basin is driven to slide out of the device, and convenience is brought to a user to take.
Drawings
FIG. 1 is a schematic overall perspective view of the present invention;
FIG. 2 is a schematic view of the overall internal front structure of the present invention;
FIG. 3 is a schematic diagram of the overall internal side structure of the present invention;
FIG. 4 is a schematic diagram of a neutral salt adjusting and feeding mechanism of the present invention;
fig. 5 is a schematic structural view of a discharging mechanism of the present invention.
In the figure: 1. a neutral salt storage bin; 2. a stirring rotating shaft; 3. adjusting quantity to observe a liquid level column; 4. a neutral salt adjusting and feeding mechanism; 5. a floating ball; 6. a transition box; 7. a funnel; 8. a material injection port; 9. a mixing bin; 10. a stirring shaft; 11. a first driving motor; 12. a second driving motor; 13. an electric box; 14. a third driving motor; 15. a salting-out device body; 16. driving the rotating shaft; 17. a connecting plate; 18. an electromagnet; 19. a piston rod; 20. a filler rod; 21. a transfer roller; 22. a transfer tank; 23. a hollow groove; 24. a transfer drum; 25. a hollow shaft; 26. a sliding shaft; 27. a permanent magnet; 28. a sliding plate; 29. a screw rod; 30. a storage chute; 31. a gear; 32. a toothed bar; 33. a toothed plate; 34. a screw nut; 35. a sealing plate; 36. a notch; 37. a sliding sleeve; 38. a filtration basin; 39. a receiving hopper; 40. a drain pipe; 41. and a discharging mechanism.
Detailed Description
The technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, but not all embodiments, and all other embodiments obtained by those skilled in the art without making any inventive effort based on the embodiments of the present invention are within the scope of protection of the present invention.
In order that the invention may be readily understood, a more complete description of the invention will be rendered by reference to the appended drawings. Several embodiments of the invention are presented. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
It will be understood that when an element is referred to as being "mounted" on another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like are used herein for illustrative purposes only.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and/or" as used herein includes any and all combinations of one or more of the associated listed items.
Referring to fig. 1-5, the present invention provides a technical solution: the salting-out device for separating and purifying animal serum protein comprises a salting-out device body 15, a neutral salt adjusting feeding mechanism 4 and a discharging mechanism 41, wherein a neutral salt storage bin 1 is fixedly arranged at the upper end of the inner side of the salting-out device body 15, a stirring rotating shaft 2 is rotatably arranged in the neutral salt storage bin 1, the outlet of the lower end of the neutral salt storage bin 1 is communicated with a transfer cylinder 24, the lower end of the neutral salt storage bin 1 is provided with the neutral salt adjusting feeding mechanism 4, the outlet of the lower end of the neutral salt adjusting feeding mechanism 4 is connected with a funnel 7, the outlet of the lower end of the funnel 7 is connected with a mixing bin 9, the upper end of the mixing bin 9 is connected with a material injection port 8 through a pipeline, a stirring shaft 10 is rotatably arranged in the mixing bin 9, a notch 36 is arranged on the lower end surface of the mixing bin 9, a storage chute 30 is internally provided with a sealing plate 35 in a sliding manner, the sealing plate 35 is matched with the notch 36, the lower end of the mixing bin 9 is provided with the discharging mechanism 41, one side surface of the salting-out device body 15 is fixedly provided with an electric box 13, the inside the electric box 13 is provided with a first driving motor 11, a second driving motor 12 and a third driving motor 14, the third driving motor 14 is in transmission connection with the stirring shaft 10, and the first driving motor 2 is in transmission connection with the stirring shaft 11;
the neutral salt adjusting and feeding mechanism 4 comprises an adjusting quantity observing liquid level column 3, a floating ball 5, a transition box 6, a driving rotating shaft 16, a connecting plate 17, an electromagnet 18, a piston rod 19, a filling rod 20, a transfer roller 21, a transfer groove 22, a hollow groove 23, a transfer cylinder 24, a hollow shaft 25, a sliding shaft 26, a permanent magnet 27 and a sliding plate 28, and the discharging mechanism 41 comprises a screw rod 29, a gear 31, a toothed bar 32, a toothed plate 33, a screw rod nut 34, a sealing plate 35, a sliding sleeve 37, a filtering basin 38, a receiving funnel 39 and a drain pipe 40.
According to the invention, preferably, the transfer roller 21 is rotatably connected to the inner side of the transfer cylinder 24, one end of the transfer roller 21 is in transmission connection with the third driving motor 14 through the driving rotating shaft 16, the other end of the transfer roller 21 is rotatably connected to the inside of the transition box 6 through the hollow shaft 25, the third driving motor 14 drives the driving rotating shaft 16 to rotate, so that the transfer roller 21 is driven to rotate through the sliding shaft 26, meanwhile, the third driving motor 14 drives the stirring rotating shaft 2 to rotate, so that neutral salt is conveniently led into the transfer groove 22, the transfer roller 21 rotates, so that the neutral salt is led into the mixing bin 9 from the hopper 7, and the second driving motor 12 drives the stirring shaft 10 to rotate, stir and mix for salting-out reaction.
According to the invention, preferably, the adjustment quantity observation liquid level column 3, the transition box 6, the hollow shaft 25 and the hollow groove 23 are communicated, one end of the piston rod 19 is slidably connected in the hollow groove 23, the other end of the piston rod 19 is fixedly connected with the center of the sliding plate 28, scale marks are arranged on the surface of the adjustment quantity observation liquid level column 3, the floating ball 5 is arranged in the adjustment quantity observation liquid level column 3, the sliding plate 28 drives the piston rod 19 to move when moving, so that liquid in the hollow groove 23 is extruded, and the liquid enters the transition box 6, so that the liquid enters the adjustment quantity observation liquid level column 3, the floating ball 5 in the adjustment quantity observation liquid level column 3 is changed, and the current capacity in the transfer groove 22 can be obtained by comparing the scales on the surfaces of the floating ball 5 and the adjustment quantity observation liquid level column 3.
In the invention, preferably, the surface of the transfer roller 21 is provided with a plurality of transfer grooves 22, a filling rod 20 is slidably arranged in the transfer grooves 22, one end of the filling rod 20 is fixedly provided with a sliding plate 28, one side surface of the sliding plate 28 is fixedly provided with a permanent magnet 27, the inner side of the transfer cylinder 24 is fixedly provided with an electromagnet 18 corresponding to the permanent magnet 27, the magnetic force of the electromagnet 18 is regulated, the permanent magnet 27 on the surface of the sliding plate 28 acts on the surface of the sliding plate 28 to drive the sliding plate 28 to slide on the surface of the sliding shaft 26, so that the filling rod 20 is driven to slide in the transfer grooves 22, the capacity in the transfer grooves 22 is regulated, and the added neutral salt amount is regulated.
In the invention, preferably, a plurality of sliding shafts 26 are arranged on one end surface of the transfer roller 21 in an array with circle centers, a connecting plate 17 is fixedly arranged at one end of each sliding shaft 26, the connecting plate 17 is fixedly connected with the driving rotating shaft 16, and a sliding plate 28 is slidably connected with each sliding shaft 26.
In the present invention, it is preferable that the screw rod 29 is screw-coupled to the screw nut 34, and the toothed plate 33 and the sealing plate 35 are fixedly coupled to the screw nut 34.
In the invention, preferably, the sliding sleeve 37 is fixedly connected to the inside of the salting-out device body 15, the toothed bar 32 is slidingly connected to the inside of the sliding sleeve 37, the gear 31 is rotatably arranged in the salting-out device body 15, the surfaces of the toothed plate 33 and the toothed bar 32 are fixedly provided with the meshing grooves, the gear 31 is meshed with the meshing grooves on the surfaces of the toothed plate 33 and the toothed bar 32, when filtering is completed, the toothed plate 33 is continuously moved, the toothed plate 33 is meshed with the gear 31 in a contact manner, and the toothed bar 32 is rotationally driven to slide in the sliding sleeve 37, so that the filter basin 38 is driven to slide out of the inside of the salting-out device, and the filtering basin is convenient for a user to take.
In the invention, preferably, one end of the toothed bar 32 is fixedly connected with the filter basin 38, the filter basin 38 and the notch 36 are arranged up and down correspondingly, and a filter screen is arranged at the bottom of the filter basin 38.
According to the invention, preferably, the lower end of the filtering basin 38 is correspondingly provided with the receiving funnel 39, one side surface of the receiving funnel 39 is fixedly connected with the drain pipe 40, the first driving motor 11 is started to drive the screw rod 29 to rotate, thereby driving the screw rod nut 34 to move, indirectly driving the toothed plate 33 and the sealing plate 35 to move, and the sealing plate 35 slides in the accommodating chute 30 to open the notch 36, so that the mixed liquid is discharged into the filtering basin 38 for filtering, and filtered water enters the receiving funnel 39 and is discharged by the drain pipe 40.
Working principle: when in use, serum is poured into the mixing bin 9 through the material injection hole 8, the magnetic force of the electromagnet 18 is regulated, the permanent magnet 27 on the surface of the sliding plate 28 is acted to drive the sliding plate 28 to slide on the surface of the sliding shaft 26, so as to drive the filling rod 20 to slide in the transfer groove 22, the capacity in the transfer groove 22 is regulated, the neutral salt quantity added is regulated, the piston rod 19 is driven to move when the sliding plate 28 moves, the liquid in the hollow groove 23 is extruded, the liquid enters the transition box 6, the liquid enters the regulating quantity observation liquid level column 3, the floating ball 5 in the regulating quantity observation liquid level column 3 is changed, the current capacity in the transfer groove 22 can be known by comparing the scales on the surfaces of the floating ball 5 and the regulating quantity observation liquid level column 3, the third driving motor 14 drives the driving rotary shaft 16 to rotate, so that the transfer roller 21 is driven to rotate through the sliding shaft 26, simultaneously, the third driving motor 14 drives the stirring rotating shaft 2 to rotate so as to conveniently guide neutral salt into the transfer groove 22, the transfer roller 21 rotates so as to drive the neutral salt to be guided into the mixing bin 9 by the hopper 7, the second driving motor 12 drives the stirring shaft 10 to rotate and stir and mix for salting out reaction, after stirring is finished, the first driving motor 11 is started to drive the screw rod 29 to rotate so as to drive the screw rod nut 34 to move, the toothed plate 33 and the sealing plate 35 are indirectly driven to move, the sealing plate 35 slides in the storage chute 30 to open the notch 36, so that mixed liquid is discharged into the filtering basin 38 for filtering, filtered water enters the receiving hopper 39 and is discharged by the drain pipe 40, the toothed plate 33 is not meshed with the gear 31 at the moment, when filtering is finished, the toothed plate 33 is continuously moved, the toothed plate 33 is meshed with the gear 31 in a contact way, the toothed rod 32 is rotationally driven to slide in the sliding sleeve 37 so as to drive the filtering basin 38 to slide out of the device, the filtering basin 38 is convenient for a user to take, and then reset.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (3)
1. The utility model provides a salting out device for animal serum protein separation and purification, includes salting out device body (15), neutral salt regulation feed mechanism (4) and discharge mechanism (41), its characterized in that: the utility model discloses a salting out device, including salting out device body (15), neutral salt storage storehouse (1) is fixed to inboard upper end, the inside rotation of neutral salt storage storehouse (1) is equipped with stirring pivot (2), neutral salt storage storehouse (1) lower extreme export and transport section of thick bamboo (24) UNICOM set up, neutral salt storage storehouse (1) lower extreme is equipped with neutral salt and adjusts feed mechanism (4), neutral salt adjusts feed mechanism (4) lower extreme exit linkage and is equipped with funnel (7), funnel (7) lower extreme exit linkage is equipped with mixed storehouse (9), mixed storehouse (9) upper end is connected with annotate material mouth (8) through the pipeline, mixed storehouse (9) inside rotation is equipped with (10), mixed storehouse (9) lower extreme surface is equipped with breach (36), mixed storehouse (9) lower extreme surface is equipped with accomodates spout (30), accomodate spout (30) inside slip and be equipped with closing plate (35) and breach (36) looks adaptation, mixed storehouse (9) lower extreme is equipped with ejection of compact mechanism (41), salt motor body (15) lower extreme is equipped with mixed storehouse (9) mix storehouse (13) and is equipped with electric motor unit (13) one side (13) fixed electric motor surface (13), electric drive case (13) are equipped with one side (13), the third driving motor (14) is in transmission connection with the stirring rotating shaft (2), the second driving motor (12) is in transmission connection with the stirring shaft (10), and the first driving motor (11) is in transmission connection with the screw rod (29);
the neutral salt adjusting and feeding mechanism (4) comprises an adjusting quantity observing liquid level column (3), a floating ball (5), a transition box (6), a driving rotating shaft (16), a connecting plate (17), an electromagnet (18), a piston rod (19), a filling rod (20), a transfer roller (21), a transfer groove (22), a hollow groove (23), a transfer cylinder (24), a hollow shaft (25), a sliding shaft (26), a permanent magnet (27) and a sliding plate (28), the discharging mechanism (41) comprises a screw rod (29), a gear (31), a toothed bar (32), a toothed plate (33), a screw nut (34), a sealing plate (35), a sliding sleeve (37), a filtering basin (38), a receiving hopper (39) and a drain pipe (40), the transfer roller (21) is rotationally connected to the inner side of the transfer cylinder (24), one end of the transfer roller (21) is in transmission connection with a third driving motor (14) through the driving rotating shaft (16), the other end of the transfer roller is rotationally connected to the inner part of the transition box (6) through a hollow shaft (25), the adjusting quantity observing liquid level column (3), the transition box (6), the hollow shaft (25) and the hollow shaft (23) are internally communicated with the hollow groove (23), one end of the hollow shaft (19) is in sliding connection with the hollow groove (23), the other end of the piston rod (19) is fixedly connected with the center of the sliding plate (28), the surface of the regulating variable observation liquid level column (3) is provided with scale marks, the inside of the regulating variable observation liquid level column (3) is provided with a floating ball (5), the surface of the transfer roller (21) is provided with a plurality of transfer grooves (22), a filling rod (20) is arranged in the transfer grooves (22) in a sliding way, one end of the filling rod (20) is fixedly provided with the sliding plate (28), one side surface of the sliding plate (28) is fixedly provided with a permanent magnet (27), the inner side of the transfer cylinder (24) is fixedly provided with an electromagnet (18) corresponding to the permanent magnet (27), one end surface of the transfer roller (21) is provided with a plurality of sliding shafts (26) in a circle center array, one end of the sliding shafts (26) is fixedly provided with a connecting plate (17), the connecting plate (17) is fixedly connected with a driving rotating shaft (16), the sliding plate (28) is slidingly connected with the sliding shafts (26), the screw rod (29) is fixedly connected with the screw rod nut (34) through threads, the toothed plate (33) and the sealing plate (35) are fixedly connected with the screw rod nut (34), the sliding sleeve (37) is fixedly connected with the screw rod body (15), the toothed device (32) is fixedly connected with the inside of a salt precipitation device body (15), the inner part (31) with a rotating device (31), the surfaces of the toothed plate (33) and the toothed bar (32) are fixedly provided with meshing grooves, and the gear (31) is in meshing connection with the meshing grooves on the surfaces of the toothed plate (33) and the toothed bar (32).
2. The salting-out apparatus for separating and purifying animal serum proteins according to claim 1, wherein: one end of the toothed bar (32) is fixedly connected with a filtering basin (38), the filtering basin (38) and the notch (36) are arranged up and down correspondingly, and a filter screen is arranged at the bottom of the filtering basin (38).
3. The salting-out apparatus for separating and purifying animal serum proteins according to claim 1, wherein: the lower end of the filtering basin (38) is correspondingly provided with a receiving funnel (39), and a drain pipe (40) is fixedly connected with one side surface of the receiving funnel (39).
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111297374.0A CN114177649B (en) | 2021-11-03 | 2021-11-03 | Salting-out device for separating and purifying animal serum protein |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202111297374.0A CN114177649B (en) | 2021-11-03 | 2021-11-03 | Salting-out device for separating and purifying animal serum protein |
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| CN114177649A CN114177649A (en) | 2022-03-15 |
| CN114177649B true CN114177649B (en) | 2023-05-16 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2627756A1 (en) * | 2010-10-14 | 2013-08-21 | Unilever PLC | Packaged particulate detergent composition |
| CN109827634A (en) * | 2019-02-20 | 2019-05-31 | 嘉兴勤慎智能技术有限公司 | A kind of liquid level sensor |
| CN209033828U (en) * | 2018-10-23 | 2019-06-28 | 浙江维康药业股份有限公司 | A kind of new type auto Alcohol-settling tank |
| CN211274473U (en) * | 2019-12-09 | 2020-08-18 | 四川农业大学 | Automatic salting-out sample injector |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2014100310B4 (en) * | 2014-03-05 | 2014-07-03 | Johali Pty Ltd | Briquetting process and product |
| CN210580929U (en) * | 2019-08-02 | 2020-05-22 | 福建汇盛生物科技有限公司 | Novel improvement fungus feed production system |
| CN211069150U (en) * | 2019-10-16 | 2020-07-24 | 南京自水源环境工程有限公司 | Solid-liquid separation settling device |
| CN212254309U (en) * | 2020-06-11 | 2020-12-29 | 武汉雅仕博科技有限公司 | Metering device for white spirit blending |
-
2021
- 2021-11-03 CN CN202111297374.0A patent/CN114177649B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2627756A1 (en) * | 2010-10-14 | 2013-08-21 | Unilever PLC | Packaged particulate detergent composition |
| CN209033828U (en) * | 2018-10-23 | 2019-06-28 | 浙江维康药业股份有限公司 | A kind of new type auto Alcohol-settling tank |
| CN109827634A (en) * | 2019-02-20 | 2019-05-31 | 嘉兴勤慎智能技术有限公司 | A kind of liquid level sensor |
| CN211274473U (en) * | 2019-12-09 | 2020-08-18 | 四川农业大学 | Automatic salting-out sample injector |
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Effective date of registration: 20230606 Address after: 237000 Hexi, Lu'an City, Anhui Province Patentee after: WEST ANHUI University Patentee after: Wuhan honger Biotechnology Co.,Ltd. Address before: 430000 floor 5, Zhongcheng Dingtai, No. 2, Shuilan Road, Donghu high tech Zone, Wuhan, Hubei Province Patentee before: Wuhan honger Biotechnology Co.,Ltd. |