CN114176215A - Method for producing organic acid calcium by adopting cordyceps militaris fermentation residual liquid - Google Patents
Method for producing organic acid calcium by adopting cordyceps militaris fermentation residual liquid Download PDFInfo
- Publication number
- CN114176215A CN114176215A CN202111473469.3A CN202111473469A CN114176215A CN 114176215 A CN114176215 A CN 114176215A CN 202111473469 A CN202111473469 A CN 202111473469A CN 114176215 A CN114176215 A CN 114176215A
- Authority
- CN
- China
- Prior art keywords
- residual liquid
- cordyceps militaris
- calcium
- organic
- filtrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 239000007788 liquid Substances 0.000 title claims abstract description 24
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 title claims abstract description 22
- 229910052791 calcium Inorganic materials 0.000 title claims abstract description 22
- 239000011575 calcium Substances 0.000 title claims abstract description 22
- 241001264174 Cordyceps militaris Species 0.000 title claims abstract description 18
- 238000000855 fermentation Methods 0.000 title claims abstract description 18
- 230000004151 fermentation Effects 0.000 title claims abstract description 18
- 150000007524 organic acids Chemical class 0.000 title claims abstract description 12
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 9
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims abstract description 16
- 239000000706 filtrate Substances 0.000 claims abstract description 11
- 239000000126 substance Substances 0.000 claims abstract description 10
- 239000002253 acid Substances 0.000 claims abstract description 8
- 230000002378 acidificating effect Effects 0.000 claims abstract description 8
- 229910000019 calcium carbonate Inorganic materials 0.000 claims abstract description 8
- 238000003795 desorption Methods 0.000 claims abstract description 8
- 239000000843 powder Substances 0.000 claims abstract description 8
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 claims abstract description 4
- 238000001035 drying Methods 0.000 claims abstract description 4
- 238000001914 filtration Methods 0.000 claims abstract description 4
- 238000010438 heat treatment Methods 0.000 claims abstract description 4
- 229920002521 macromolecule Polymers 0.000 claims abstract description 4
- 239000011347 resin Substances 0.000 claims abstract description 4
- 229920005989 resin Polymers 0.000 claims abstract description 4
- 238000001179 sorption measurement Methods 0.000 claims abstract description 4
- 238000003756 stirring Methods 0.000 claims abstract description 4
- KQLDDLUWUFBQHP-UHFFFAOYSA-N Cordycepin Natural products C1=NC=2C(N)=NC=NC=2N1C1OCC(CO)C1O KQLDDLUWUFBQHP-UHFFFAOYSA-N 0.000 claims description 7
- OFEZSBMBBKLLBJ-BAJZRUMYSA-N cordycepin Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)C[C@H]1O OFEZSBMBBKLLBJ-BAJZRUMYSA-N 0.000 claims description 7
- OFEZSBMBBKLLBJ-UHFFFAOYSA-N cordycepine Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(CO)CC1O OFEZSBMBBKLLBJ-UHFFFAOYSA-N 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 7
- 239000003957 anion exchange resin Substances 0.000 claims description 3
- 239000000919 ceramic Substances 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims description 2
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 claims 4
- 239000002126 C01EB10 - Adenosine Substances 0.000 claims 2
- 229960005305 adenosine Drugs 0.000 claims 2
- 238000012258 culturing Methods 0.000 claims 2
- 239000002775 capsule Substances 0.000 abstract description 3
- 238000004134 energy conservation Methods 0.000 abstract description 3
- 230000007613 environmental effect Effects 0.000 abstract description 3
- 238000004064 recycling Methods 0.000 abstract description 3
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000006227 byproduct Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 208000002381 Brain Hypoxia Diseases 0.000 description 1
- 206010008111 Cerebral haemorrhage Diseases 0.000 description 1
- 206010008132 Cerebral thrombosis Diseases 0.000 description 1
- 241000190633 Cordyceps Species 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 201000001429 Intracranial Thrombosis Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical group [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 208000010125 myocardial infarction Diseases 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 150000003833 nucleoside derivatives Chemical class 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/16—Inorganic salts, minerals or trace elements
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/16—Purine radicals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Polymers & Plastics (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Mycology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Inorganic Chemistry (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention relates to a method for producing organic acid calcium by cordyceps militaris fermentation residual liquid, which comprises the following steps: filtering the cordyceps militaris fermentation residual liquid to remove macromolecular substances to obtain filtrate; adsorbing acidic substances in the filtrate by using resin to obtain adsorption residual liquid; desorbing the adsorbed acidic substance by adopting an ammonia hydroxide solution to obtain a desorption solution; heating the desorption solution, concentrating under reduced pressure, adding calcium carbonate powder, and stirring to obtain an organic calcium carbonate concentrated solution; drying the organic calcium acid concentrated solution to obtain organic calcium acid powder. In the invention, the obtained organic acid calcium can be used as a tablet or capsule calcium nutriment to realize resource recycling, thereby achieving the purposes of energy conservation and environmental protection.
Description
Technical Field
The invention relates to a method for producing organic acid calcium by cordyceps militaris fermentation residual liquid.
Background
Cordyceps militaris (Cordyceps militaris), also known as Cordyceps militaris, is a genus of Cordyceps of Clavipitaceae, order of Ascomycotina. The produced cordycepin can regulate immunity, resist inflammation, bacteria, viruses, tumors, oxidation and the like; the produced nucleoside substance has the effects of reducing blood viscosity, improving blood oxygen supply capability, and preventing thrombosis, heart and brain anoxia, etc.; the produced cordycepic acid can inhibit growth of various pathogenic bacteria, and can be used for preventing and treating cerebral thrombosis, cerebral hemorrhage, myocardial infarction, long-term failure, etc.; the produced various amino acids and small molecular peptides can be directly digested and absorbed by human body as nutrient substances.
At present, the cordyceps militaris has two production methods, namely solid culture and liquid fermentation, and in the process of liquid fermentation, after cordycepin is obtained, other small molecular organic acids, such as nucleotides, cordycepic acid, amino acid and other large-scale byproducts exist in the remaining fermentation residual liquid. These byproducts are usually not reused, resulting in waste of resources.
Disclosure of Invention
In order to overcome the technical problems in the prior art, the invention provides a method for producing organic acid calcium by cordyceps militaris fermentation residual liquid, which comprises the following steps:
(a) filtering the cordyceps militaris fermentation residual liquid to remove macromolecular substances to obtain filtrate;
(b) adsorbing acidic substances in the filtrate by using resin to obtain adsorption residual liquid;
(c) desorbing the adsorbed acidic substance by adopting an ammonia hydroxide solution to obtain a desorption solution;
(d) heating the desorption solution, concentrating under reduced pressure, adding calcium carbonate powder, and stirring to obtain an organic calcium carbonate concentrated solution;
(e) drying the organic calcium acid concentrated solution to obtain organic calcium acid powder.
Further, in (a), the fermentation residue is filtered by using a plate frame and a ceramic membrane.
Further, in (b), the filtrate is adsorbed by using an anion exchange resin column of the hydroxyl group type.
Further, the above-described method further comprises step (f);
(f) used for adsorbing residual liquid to extract cordycepin.
The invention has the beneficial effects that: in the invention, the obtained organic acid calcium can be used as a tablet or capsule calcium nutriment to realize resource recycling, thereby achieving the purposes of energy conservation and environmental protection.
Detailed Description
The present invention is further illustrated by the following examples, which are only a part of the examples of the present invention, and these examples are only for explaining the present invention and do not limit the scope of the present invention.
The invention provides a method for producing organic acid calcium by cordyceps militaris fermentation residual liquid, which comprises the following steps:
(a) filtering the cordyceps militaris fermentation residual liquid to remove macromolecular substances to obtain filtrate;
specifically, a plate frame and a ceramic membrane are adopted to filter the fermentation residual liquid.
(b) Adsorbing acidic substances in the filtrate by using resin to obtain adsorption residual liquid;
specifically, the filtrate is adsorbed by using an hydroxyl anion exchange resin column;
(c) desorbing the adsorbed acidic substance by adopting an ammonia hydroxide solution to obtain a desorption solution;
(d) heating the desorption solution, concentrating under reduced pressure, adding calcium carbonate powder, and stirring to obtain an organic calcium carbonate concentrated solution;
(e) drying the organic calcium acid concentrated solution to obtain organic calcium acid powder.
In the invention, the obtained organic acid calcium can be used as a tablet or capsule calcium nutriment to realize resource recycling, thereby achieving the purposes of energy conservation and environmental protection.
Further, the method further comprises step (f);
(f) used for adsorbing residual liquid to extract cordycepin.
Although the present invention has been described with reference to a preferred embodiment, it should be understood that various changes, substitutions and alterations can be made herein without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (4)
1. A method for producing organic acid calcium by cordyceps militaris fermentation residual liquid is characterized by comprising the following steps:
(a) filtering the cordyceps militaris fermentation residual liquid to remove macromolecular substances to obtain filtrate;
(b) adsorbing acidic substances in the filtrate by using resin to obtain adsorption residual liquid;
(c) desorbing the adsorbed acidic substance by adopting an ammonia hydroxide solution to obtain a desorption solution;
(d) heating the desorption solution, concentrating under reduced pressure, adding calcium carbonate powder, and stirring to obtain an organic calcium carbonate concentrated solution;
(e) drying the organic calcium acid concentrated solution to obtain organic calcium acid powder.
2. The method for producing the organic acid calcium by adopting the cordyceps militaris fermentation residual liquid, which is characterized by comprising the following steps of: in the step (a), a plate frame and a ceramic membrane are adopted to filter the fermentation residual liquid.
3. The method for culturing cordycepin and adenosine contents in Cordyceps militaris as claimed in claim 1, wherein the method comprises the following steps: in the step (b), the filtrate is adsorbed by using an anion exchange resin column of the hydroxyl group type.
4. The method for culturing cordycepin and adenosine contents in Cordyceps militaris as claimed in claim 1, wherein the method comprises the following steps: further comprising step (f);
(f) used for adsorbing residual liquid to extract cordycepin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111473469.3A CN114176215A (en) | 2021-11-29 | 2021-11-29 | Method for producing organic acid calcium by adopting cordyceps militaris fermentation residual liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111473469.3A CN114176215A (en) | 2021-11-29 | 2021-11-29 | Method for producing organic acid calcium by adopting cordyceps militaris fermentation residual liquid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114176215A true CN114176215A (en) | 2022-03-15 |
Family
ID=80542315
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111473469.3A Pending CN114176215A (en) | 2021-11-29 | 2021-11-29 | Method for producing organic acid calcium by adopting cordyceps militaris fermentation residual liquid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114176215A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1946851A (en) * | 2004-04-26 | 2007-04-11 | 帝斯曼知识产权资产管理有限公司 | Process for the preparation of calcium-d-pantothenate |
| CN103483021A (en) * | 2013-08-27 | 2014-01-01 | 王辉 | Method for preparing liquid fertilizer by converting Cordyceps fermentation residual liquid with EM (effective microorganism) flora |
| CN112021453A (en) * | 2020-09-10 | 2020-12-04 | 科赛德(广东)生物技术有限公司 | Method for synthesizing composite organic acid calcium by producing organic acid through microbial fermentation |
-
2021
- 2021-11-29 CN CN202111473469.3A patent/CN114176215A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1946851A (en) * | 2004-04-26 | 2007-04-11 | 帝斯曼知识产权资产管理有限公司 | Process for the preparation of calcium-d-pantothenate |
| CN103483021A (en) * | 2013-08-27 | 2014-01-01 | 王辉 | Method for preparing liquid fertilizer by converting Cordyceps fermentation residual liquid with EM (effective microorganism) flora |
| CN112021453A (en) * | 2020-09-10 | 2020-12-04 | 科赛德(广东)生物技术有限公司 | Method for synthesizing composite organic acid calcium by producing organic acid through microbial fermentation |
Non-Patent Citations (1)
| Title |
|---|
| 黄朝霞等: "吸附法提取乳酸新工艺的研究", 离子交换与吸附, no. 1, pages 18 * |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20220315 |