CN114163265A - Method for treating rifamycin residues in mushroom dregs by ultrasonic-alkaline heat combination - Google Patents
Method for treating rifamycin residues in mushroom dregs by ultrasonic-alkaline heat combination Download PDFInfo
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- CN114163265A CN114163265A CN202111356512.8A CN202111356512A CN114163265A CN 114163265 A CN114163265 A CN 114163265A CN 202111356512 A CN202111356512 A CN 202111356512A CN 114163265 A CN114163265 A CN 114163265A
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- C05—FERTILISERS; MANUFACTURE THEREOF
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- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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Abstract
The invention belongs to the technical field of antibiotic treatment, and particularly discloses a method for treating rifamycin residues in mushroom dregs by ultrasonic-alkaline heat combination, which comprises the following steps: adjusting the water content: weighing quantitative rifamycin bacterial residues, adding deionized water, and adjusting the water content of the bacterial residues to 95%; alkali liquor treatment: adding 4M NaOH into the fungus dregs, uniformly stirring, and adjusting the pH value of the fungus dregs to 8-12; hydrothermal ultrasonic treatment: and (3) placing the alkaline fungi residues in a constant-temperature water bath at 80-100 ℃, and carrying out ultrasonic treatment for 10-120 min. The method is simple and efficient, does not need excessive chemical reagents and harsh conditions, does not produce secondary pollution, is a clean and sustainable utilization technology, is beneficial to popularization and batch treatment, can realize waste recycling through a composting process, and can realize the purposes of reduction and recycling by using compost finished products as organic fertilizers.
Description
Technical Field
The invention belongs to the technical field of antibiotic treatment, and particularly relates to a method for treating rifamycin residues in mushroom dregs by ultrasonic-alkaline heat combination.
Background
Rifamycin is often used together with other anti-tubercular drugs for treating various tuberculosis and treating severe infection of drug-resistant staphylococcus aureus, a large amount of rifamycin bacterial residues are generated in the production and fermentation processes, the current treatment and disposal modes of antibiotic bacterial residues are mainly incineration, landfill technology and the like, but the water content of the rifamycin bacterial residues is as high as 70-80%, the heat value is low, the operation energy consumption and the cost are high, the rifamycin bacterial residues cannot be treated by a conventional incineration mode, meanwhile, the incineration treatment of the antibiotic bacterial residues must strictly execute the hazardous waste incineration pollution control standard, and if the incineration is improper, the multi-medium transmission of toxic substances such as dioxin and the like is easily caused, and secondary pollution is caused. In addition, the rifamycin bacterial slag has high organic matter content, and cells also contain a large amount of organic matter, so that safe landfill is directly performed, and the problems of large occupied area, high disposal cost and secondary pollution exist, and resource waste is caused. In actual production, the mushroom dregs often contain a large amount of organic matters including proteins, unused culture media and the like, and can be used as nutrient substances for plant growth, waste can be changed into valuable substances through a composting process, compost finished products can be recycled as organic fertilizers, and finally the purposes of reduction and recycling are achieved. At present, the research on the treatment process of rifamycin and its mushroom dregs is less, and the mushroom dregs contain some harmful substances, and considering the particularity of the rifamycin mushroom dregs, an efficient, safe and reliable pretreatment process for harmless treatment of the rifamycin mushroom dregs is urgently needed, so that the rifamycin and its mushroom dregs can be conveniently subjected to resource utilization such as composting.
Accordingly, further developments and improvements are still needed in the art.
Disclosure of Invention
Aiming at various defects in the prior art and solving the problems, a method for treating rifamycin residues in mushroom dregs by ultrasonic-alkali heat combination is provided. The invention provides the following technical scheme:
a method for treating rifamycin residues in mushroom dregs by ultrasonic-alkaline heat combination comprises the following steps:
s1, adjusting the water content: weighing quantitative rifamycin bacterial residues, adding deionized water, and adjusting the water content of the bacterial residues to 95%;
s2, alkali liquor treatment: adding 4M NaOH into the fungus dregs, uniformly stirring, and adjusting the pH value of the fungus dregs to 8-12;
s3, hydrothermal ultrasonic treatment: and (3) placing the alkaline fungi residues in a constant-temperature water bath at 80-100 ℃, and carrying out ultrasonic treatment for 10-120 min.
Further, the ultrasonic intensity used for the ultrasonic treatment in S3 is 1.5-3.0W/mL.
Further, the ultrasonic intensity used for the ultrasonic treatment in S3 is 2.0-2.5W/mL.
Further, in the ultrasonic treatment in S3, the ultrasonic intensity was 2.5W/mL.
Further, the ultrasonic treatment time in the step S3 is 60-120 min.
Further, the sonication time in S3 was 120 min.
Further, the temperature of the constant temperature water bath in the step S3 is 90-100 ℃.
Further, the temperature of the constant-temperature water bath in S3 was 90 ℃.
Further, the pH of the mushroom dregs is adjusted to 10 to 11 in the step S2.
Further, in the above step S2, the pH of the mushroom dregs is adjusted to 11.
Has the advantages that:
1. compared with other treatment modes of the bacteria residues, the ultrasonic-alkali-heat combined treatment provided by the invention is an economic and effective treatment method, can avoid the risk of leaching a large amount of ions caused by conventional transition metal activated hydrogen peroxide or persulfate and other advanced oxidation technologies, and is beneficial to resource utilization of the subsequent rifamycin bacteria residues;
2. the treatment process has relatively mild conditions, can ensure that the destruction degree of the nutrient contents of the organic matters in the mushroom dregs is low on the premise of efficiently removing the rifamycin residues in the rifamycin mushroom dregs, is favorable for realizing the recovery of nutrient substances and realizing the selective removal of the organic matters in the mushroom dregs;
3. the method is simple and efficient, does not need excessive chemical reagents and harsh conditions, does not produce secondary pollution, and is a clean and sustainable utilization technology;
4. the method effectively utilizes the characteristic of high water content of the rifamycin bacterial residues, takes water as a good heat conduction and storage medium, and combines alkali-heat ultrasonic treatment to quickly raise the temperature of the bacterial residues, so that mycelium cells are easily damaged, intracellular rifamycin and intracellular organic matters are sufficiently released, and the degradation process is accelerated;
5. the method has simple treatment means, is beneficial to popularization and batch treatment, can change waste into valuable through the composting process, and can recycle the compost finished product as an organic fertilizer, thereby finally realizing the purposes of reduction and recycling.
Drawings
FIG. 1 is a schematic flow chart of a method for treating rifamycin residues in mushroom dregs by ultrasonic-alkaline-thermal combination in an embodiment of the present invention;
FIG. 2 is a graph of comparative example degradation performance of rifamycin during processing of rifamycin bacterial sludge in various ways in embodiments of the invention.
Detailed Description
In order to make the technical solutions of the present invention better understood, the following description of the technical solutions of the present invention with reference to the accompanying drawings of the present invention is made clearly and completely, and other similar embodiments obtained by a person of ordinary skill in the art without any creative effort based on the embodiments in the present application shall fall within the protection scope of the present application. In addition, directional terms such as "upper", "lower", "left", "right", etc. in the following embodiments are directions with reference to the drawings only, and thus, the directional terms are used for illustrating the present invention and not for limiting the present invention.
As shown in fig. 1, a method for treating rifamycin residues in mushroom dregs by ultrasonic-alkaline heat combination comprises the following steps:
s1, adjusting the water content: weighing quantitative rifamycin bacterial residues, adding deionized water, and adjusting the water content of the bacterial residues to 95%;
s2, alkali liquor treatment: adding 4M NaOH into the fungus dregs, uniformly stirring, and adjusting the pH value of the fungus dregs to 8-12;
s3, hydrothermal ultrasonic treatment: and (3) placing the alkaline fungi residues in a constant-temperature water bath at 80-100 ℃, and carrying out ultrasonic treatment for 10-120 min.
Further, the ultrasonic intensity used for the ultrasonic treatment in S3 is 1.5-3.0W/mL.
Further, the ultrasonic intensity used for the ultrasonic treatment in S3 is 2.0-2.5W/mL.
Further, in the ultrasonic treatment in S3, the ultrasonic intensity was 2.5W/mL.
Further, the ultrasonic treatment time in the step S3 is 60-120 min.
Further, the sonication time in S3 was 120 min.
Further, the temperature of the constant temperature water bath in the step S3 is 90-100 ℃.
Further, the temperature of the constant-temperature water bath in S3 was 90 ℃.
Further, the pH of the mushroom dregs is adjusted to 10 to 11 in the step S2.
Further, in the above step S2, the pH of the mushroom dregs is adjusted to 11.
Specifically, comparative experimental studies were conducted on temperature, pH and ultrasound intensity parameters, respectively.
Example 1 Effect of reaction temperature on degradation efficiency
Weighing a certain amount of fresh rifamycin bacterial slag, adding deionized water to adjust the water content of the bacterial slag to 95%, adding prepared 4M NaOH into the bacterial slag with the adjusted water content, uniformly stirring, adjusting the pH to 11, respectively placing the bacterial slag with the adjusted water content at 80, 90 and 100 ℃, adjusting the ultrasonic intensity in an ultrasonic processor to be 2.5W/mL, and starting to react for 120 min.
The experimental result shows that the reaction temperature has great influence on the ultrasonic-alkali heat combined treatment of the rifamycin bacterial residues, as shown in table 1. When the pH value is 11, the ultrasonic intensity is 2.5W/mL, and the reaction temperature is 90 ℃, the degradation rate of the rifamycin in the mushroom dregs is 99.80%.
TABLE 1 influence of reaction temperature on the rifamycin degradation efficiency in the mushroom dregs
| Reaction temperature (. degree.C.) | 80 | 90 | 100 |
| Degradation Rate (%) | 88.56 | 99.80 | 100 |
Example 2 Effect of pH on degradation efficiency
Weighing a certain amount of fresh rifamycin bacterial residues, adding deionized water to adjust the water content of the bacterial residues to 95%, adding prepared 4M NaOH into the bacterial residues with the water content adjusted, uniformly stirring, adjusting the pH to 8, 10, 11 and 12, respectively placing the bacterial residues with the water content adjusted at 90 ℃, adjusting the ultrasonic intensity in an ultrasonic processor to be 2.5W/mL, and starting to react for 120 min. The experimental results show that the pH value has a great influence on the ultrasonic-alkaline heat combined treatment of the rifamycin bacterial residues, as shown in Table 2. When the reaction temperature is 90 ℃, the ultrasonic intensity is 2.5W/mL, and the pH value is 11, the degradation rate of the rifamycin in the mushroom dregs is 99.80 percent, which is obviously better than that of the rifamycin with the pH values of 8 and 10.
TABLE 2 influence of pH on the rifamycin degradation efficiency in the bacterial sludge
| pH | 8 | 10 | 11 | 12 |
| Degradation Rate (%) | 74.56 | 87.69 | 99.80 | 100 |
Example 3 Effect of ultrasound intensity on degradation efficiency
Weighing a certain amount of fresh rifamycin bacterial residues, adding deionized water to adjust the water content of the bacterial residues to 95%, adding prepared 4M NaOH into the bacterial residues with the water content adjusted, uniformly stirring, adjusting the pH to 11, respectively placing the bacterial residues with the water content adjusted at 90 ℃, adjusting the ultrasonic intensity in an ultrasonic processor to be 1.5, 2.0, 2.5 and 3.0W/mL, and starting to react for 120 min. The experimental results show that the pH value has a great influence on the ultrasonic-alkaline heat combined treatment of the rifamycin bacterial residues, as shown in Table 3. When the reaction temperature is 90 ℃, the ultrasonic intensity is 2.5W/mL, and the pH value is 11, the degradation rate of the rifamycin in the mushroom dregs is 99.80%.
TABLE 3 influence of ultrasound intensity on rifamycin degradation efficiency in mushroom dregs
| Ultrasonic intensity (W/mL) | 1.5 | 2.0 | 2.5 | 3.0 |
| Degradation Rate (%) | 84.29 | 95.77 | 99.80 | 100 |
Comparative example 1
Weighing a certain amount of fresh rifamycin bacterial residues, adding deionized water to adjust the water content of the bacterial residues to 95%, adding prepared 4M NaOH into the bacterial residues with the water content adjusted, adjusting the pH to 11, stirring uniformly, respectively placing the bacterial residues with the water content adjusted at 90 ℃, and starting to react for 120 min. And (3) investigating the degradation performance of the rifamycin in the mushroom dregs by the alkali-heat treatment in the environment without ultrasonic treatment.
Comparative example 2
Weighing a certain amount of fresh rifamycin bacterial slag, adding deionized water to adjust the water content of the bacterial slag to 95%, adding prepared 4M NaOH into the bacterial slag with the water content adjusted, adjusting the pH to 11, stirring uniformly, carrying out the reaction in an ultrasonic processor under the condition of adjusting the ultrasonic intensity to 2.5W/mL without heating, and starting the reaction for 120 min. And (3) investigating the degradation performance of the rifamycin in the bacterial residues by ultrasonic-alkali treatment in a non-heat treatment environment.
Comparative example 3
Weighing a certain amount of fresh rifamycin bacterial residues, adding deionized water to adjust the water content of the bacterial residues to 95%, respectively placing the bacterial residues with the adjusted water content at 90 ℃, carrying out the reaction under the condition of adjusting the ultrasonic intensity to 2.5W/mL in an ultrasonic processor, and starting the reaction for 120 min. And (3) inspecting the degradation performance of the ultrasonic-heat treatment on the rifamycin in the mushroom dregs in the non-alkaline treatment environment.
Under the reaction conditions described in the above comparative examples 1, 2, and 3, the degradation rates of rifamycin in the mushroom dregs were 75.62%, 6.91%, and 31.48%, respectively (as shown in fig. 2), while in the examples, the degradation rate of rifamycin in the mushroom dregs was 99.80% at a reaction temperature of 90 ℃, an ultrasonic intensity of 2.5W/mL, and a pH of 11, which completely met the degradation requirements, and the conditions were relatively mild, and were convenient for batch execution. The results of the combined examples and comparative examples show that the efficiency of rifamycin degradation in the ultrasonic-alkaline-thermal combined treatment of the mushroom dregs is superior to that of the alkali, thermal-individual treatment and ultrasonic-individual treatment alone.
The ultrasonic technology has the characteristics of no pollution and high decomposition speed, can destroy the rigid cell wall of the rifamycin bacterial residues, and can destroy cells in a short time to release intracellular organic matters. The alkaline heat treatment can effectively utilize the characteristic of high water content of the rifamycin bacterial residues, and takes water as a good heat conduction and storage medium to quickly raise the temperature of the bacterial residues. The rifamycin is easy to be converted into an ionic state under an alkaline condition, so that osmotic pressure in cells can be destroyed, mycelium cells can be dissolved to a certain extent, the mycelium cells are easy to be destroyed under the assistance of heating, intracellular rifamycin and intracellular organic matters can be sufficiently released, and the rifamycin and the intracellular organic matters can be contacted with water molecules more favorably and then degraded and destroyed. Therefore, the ultrasonic-alkali-heat combined treatment can more efficiently destroy cell walls of cells and increase the dissolution of soluble substances in the bacteria residues, so that organic pollutants in a bacteria residue system can be better removed, a new way is provided for economically and safely treating antibiotic bacteria residues in a harmless manner, and a technical support is provided for the resource utilization of subsequent rifamycin bacteria residues.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
The present invention has been described in detail, and it should be understood that the detailed description and specific examples, while indicating the preferred embodiment of the invention, are intended for purposes of illustration only and are not intended to limit the scope of the invention.
Claims (10)
1. A method for treating rifamycin residues in mushroom dregs by ultrasonic-alkaline heat combination is characterized by comprising the following steps:
s1, adjusting the water content: weighing quantitative rifamycin bacterial residues, adding deionized water, and adjusting the water content of the bacterial residues to 95%;
s2, alkali liquor treatment: adding 4M NaOH into the fungus dregs, uniformly stirring, and adjusting the pH value of the fungus dregs to 8-12;
s3, hydrothermal ultrasonic treatment: and (3) placing the alkaline fungi residues in a constant-temperature water bath at 80-100 ℃, and carrying out ultrasonic treatment for 10-120 min.
2. The method for the combined ultrasonic-alkaline-thermal treatment of the rifamycin residues in the mushroom dregs as claimed in claim 1, wherein the ultrasonic intensity used for the ultrasonic treatment in S3 is 1.5-3.0W/mL.
3. The method for the combined ultrasonic-alkaline-thermal treatment of the rifamycin residues in the mushroom dregs as claimed in claim 1, wherein the ultrasonic intensity used for the ultrasonic treatment in S3 is 2.0-2.5W/mL.
4. The method for the combined ultrasonic-alkaline-thermal treatment of the rifamycin residues in the mushroom dregs as claimed in claim 1, wherein the ultrasonic intensity used for the ultrasonic treatment in S3 is 2.5W/mL.
5. The method for the combined ultrasonic-alkaline-thermal treatment of the rifamycin residues in the mushroom dregs as claimed in claim 1, wherein the ultrasonic treatment time in the step S3 is 60-120 min.
6. The method for the combined ultrasonic-alkaline-thermal treatment of the rifamycin residues in the mushroom dregs as claimed in claim 1, wherein the ultrasonic treatment time in S3 is 120 min.
7. The method for the ultrasonic-alkaline-thermal combined treatment of the rifamycin residues in the mushroom dregs as claimed in claim 1, wherein the temperature of the constant-temperature water bath in the step S3 is 90-100 ℃.
8. The method for the combined ultrasonic-alkaline-thermal treatment of the rifamycin residues in the mushroom dregs as claimed in claim 1, wherein the temperature of the constant-temperature water bath in the step S3 is 90 ℃.
9. The method for the combined ultrasonic-alkaline-thermal treatment of the rifamycin residues in the mushroom dregs as claimed in claim 1, wherein the pH of the mushroom dregs is adjusted to 10-11 in S2.
10. The method of claim 1, wherein the pH of the mushroom dregs is adjusted to 11 in S2.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115215687A (en) * | 2022-07-01 | 2022-10-21 | 青岛农业大学 | Harmless treatment method for cyclosporine A high-residue bacterial slag |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103820315A (en) * | 2014-03-07 | 2014-05-28 | 河北科技大学 | Device and method for harmless treatment of cynnematin germ dregs |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103820315A (en) * | 2014-03-07 | 2014-05-28 | 河北科技大学 | Device and method for harmless treatment of cynnematin germ dregs |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115215687A (en) * | 2022-07-01 | 2022-10-21 | 青岛农业大学 | Harmless treatment method for cyclosporine A high-residue bacterial slag |
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