CN114162977A - Biological synergistic activator capable of improving microbial activity - Google Patents
Biological synergistic activator capable of improving microbial activity Download PDFInfo
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- CN114162977A CN114162977A CN202111515204.5A CN202111515204A CN114162977A CN 114162977 A CN114162977 A CN 114162977A CN 202111515204 A CN202111515204 A CN 202111515204A CN 114162977 A CN114162977 A CN 114162977A
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- 239000012190 activator Substances 0.000 title claims abstract description 53
- 230000002195 synergetic effect Effects 0.000 title claims abstract description 50
- 230000000813 microbial effect Effects 0.000 title claims abstract description 29
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 76
- 239000002921 fermentation waste Substances 0.000 claims abstract description 48
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 38
- 238000000855 fermentation Methods 0.000 claims abstract description 35
- 230000004151 fermentation Effects 0.000 claims abstract description 35
- POJWUDADGALRAB-UHFFFAOYSA-N allantoin Chemical compound NC(=O)NC1NC(=O)NC1=O POJWUDADGALRAB-UHFFFAOYSA-N 0.000 claims abstract description 30
- 235000012907 honey Nutrition 0.000 claims abstract description 27
- 229920002472 Starch Polymers 0.000 claims abstract description 24
- 235000019698 starch Nutrition 0.000 claims abstract description 24
- 239000008107 starch Substances 0.000 claims abstract description 24
- 239000006179 pH buffering agent Substances 0.000 claims abstract description 20
- 239000000843 powder Substances 0.000 claims abstract description 20
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 16
- 108090000790 Enzymes Proteins 0.000 claims abstract description 16
- 102000004190 Enzymes Human genes 0.000 claims abstract description 16
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 16
- POJWUDADGALRAB-PVQJCKRUSA-N Allantoin Natural products NC(=O)N[C@@H]1NC(=O)NC1=O POJWUDADGALRAB-PVQJCKRUSA-N 0.000 claims abstract description 15
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 15
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 15
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 15
- 229960000458 allantoin Drugs 0.000 claims abstract description 15
- 230000003197 catalytic effect Effects 0.000 claims abstract description 13
- 229920002261 Corn starch Polymers 0.000 claims abstract description 12
- 239000008120 corn starch Substances 0.000 claims abstract description 12
- 150000005856 steroid saponins Chemical class 0.000 claims abstract description 12
- 239000000203 mixture Substances 0.000 claims abstract description 11
- 235000013312 flour Nutrition 0.000 claims abstract description 10
- 239000007788 liquid Substances 0.000 claims description 75
- 238000002156 mixing Methods 0.000 claims description 65
- 239000011259 mixed solution Substances 0.000 claims description 31
- 239000010865 sewage Substances 0.000 claims description 18
- 238000001816 cooling Methods 0.000 claims description 16
- 238000010438 heat treatment Methods 0.000 claims description 16
- 239000000243 solution Substances 0.000 claims description 16
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 11
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 11
- 235000019797 dipotassium phosphate Nutrition 0.000 claims description 11
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical group O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 11
- 239000001509 sodium citrate Substances 0.000 claims description 11
- 238000000227 grinding Methods 0.000 claims description 10
- 238000007789 sealing Methods 0.000 claims description 8
- 230000001954 sterilising effect Effects 0.000 claims description 8
- 238000003860 storage Methods 0.000 claims description 8
- 239000013543 active substance Substances 0.000 claims description 6
- 239000002994 raw material Substances 0.000 claims description 6
- 239000003054 catalyst Substances 0.000 claims description 4
- 229920000936 Agarose Polymers 0.000 claims description 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 3
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 3
- 239000001888 Peptone Substances 0.000 claims description 3
- 108010080698 Peptones Proteins 0.000 claims description 3
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 238000000034 method Methods 0.000 claims description 3
- 235000013379 molasses Nutrition 0.000 claims description 3
- 239000006174 pH buffer Substances 0.000 claims description 3
- 235000019319 peptone Nutrition 0.000 claims description 3
- 229910052700 potassium Inorganic materials 0.000 claims description 3
- 239000011591 potassium Substances 0.000 claims description 3
- 230000002708 enhancing effect Effects 0.000 claims 7
- 239000003344 environmental pollutant Substances 0.000 abstract description 9
- 231100000719 pollutant Toxicity 0.000 abstract description 9
- 230000000694 effects Effects 0.000 abstract description 8
- 244000005700 microbiome Species 0.000 abstract description 8
- 239000002351 wastewater Substances 0.000 abstract description 6
- 238000012545 processing Methods 0.000 abstract description 5
- 238000006065 biodegradation reaction Methods 0.000 abstract description 4
- 239000003814 drug Substances 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 239000010802 sludge Substances 0.000 abstract description 2
- 238000012546 transfer Methods 0.000 abstract description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 10
- 238000002360 preparation method Methods 0.000 description 7
- 239000000126 substance Substances 0.000 description 4
- 230000001766 physiological effect Effects 0.000 description 3
- 230000001988 toxicity Effects 0.000 description 3
- 231100000419 toxicity Toxicity 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 239000010842 industrial wastewater Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 229910052793 cadmium Inorganic materials 0.000 description 1
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- JOPOVCBBYLSVDA-UHFFFAOYSA-N chromium(6+) Chemical compound [Cr+6] JOPOVCBBYLSVDA-UHFFFAOYSA-N 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000000498 cooling water Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000037353 metabolic pathway Effects 0.000 description 1
- 230000007269 microbial metabolism Effects 0.000 description 1
- 150000002825 nitriles Chemical class 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
- 150000003568 thioethers Chemical class 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
Abstract
The invention discloses a biological synergistic activator capable of improving microbial activity, which comprises a carbon source, a fermentation mixed liquor, a nitrogen source, steroid saponin, a pH buffering agent and a biological catalytic enzyme, wherein the carbon source is corn starch, the nitrogen source is a mixture consisting of allantoin, bean flour and yeast powder, and the fermentation mixed liquor is a mixture consisting of a honey fermentation waste liquor and a starch fermentation waste liquor. The biological synergistic activator provided by the invention can convert various pollutants into easily degradable micromolecular organic matters, and the removal rate of biodegradation of the pollutants is improved; the processing capacity and the impact load resistance of the microbial system can be further improved; the population and the quantity of functional microorganisms of the biochemical system are greatly improved, the mass transfer speed of the pollutants which are difficult to degrade in the wastewater is greatly improved, and the processing capacity of the biochemical system is obviously improved; the effect of the system can be maintained only by using a very small amount of supplementary medicament, the use of other medicaments can be reduced, the sludge production amount is reduced, and the comprehensive treatment cost of the system is reduced.
Description
Technical Field
The invention relates to the field of industrial sewage treatment, in particular to a biological synergistic activator capable of improving microbial activity.
Background
The industrial wastewater treatment mainly removes suspended matters, sulfides, petroleum, cyanides, hexavalent chromium, lead, cadmium and other substances with high chemical oxygen demand. Since the establishment of new China, with the continuous development of national economy, more industrial departments are provided, the scale of a factory is enlarged, the discharge amount of wastewater and the types of pollutants are increased day by day, and the wastewater is discharged into nearby water bodies without being treated to pollute the water quality. The quantity and quality of industrial waste water vary greatly according to products and production processes, and typical data are not suitable for field investigation. The industry uses a large amount of cooling water, most of which are contacted with different materials, and the water quality of the used water generally has little change, but the water temperature rises to some extent. On the contrary, the liquid and washing wastewater (except wastewater in industries such as paper making, textile, printing and dyeing) used in the production process generally have small water quantity, but the water quality is extremely complex and the concentration is generally high.
When the industrial sewage treatment machine is treated by adopting a biodegradation method, the activity of biological bacterial colonies is insufficient, the biodegradation efficiency of pollutants in the industrial sewage is low, the physiological activity, the toxicity resistance and the impact resistance of microorganisms are low, and the population and the number of the microorganisms cannot be rapidly multiplied, so that the treatment capacity of a biochemical system is obviously influenced.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention provides a biological synergistic activator capable of improving the activity of microorganisms, which obviously improves the living environment of the microorganisms of a biochemical system, enhances the physiological activity and the toxicity resistance of the microorganisms and can further improve the processing capacity and the impact load resistance of the microorganism system.
In order to solve the technical problems, the invention provides the following technical scheme:
a biological synergistic activator with the function of improving the activity of microorganisms comprises the following components in percentage by mass: 20-40 parts of carbon source, 50-80 parts of fermentation mixed liquor, 15-30 parts of nitrogen source, 20-50 parts of steroid saponin, 2-11 parts of pH buffer and 0.5-3 parts of biological catalytic enzyme, wherein the carbon source is corn starch, the nitrogen source is a mixture consisting of allantoin, bean flour and yeast powder, and the fermentation mixed liquor is a mixture consisting of honey fermentation waste liquor and starch fermentation waste liquor.
As a preferable technical scheme of the invention, the honey fermentation waste liquid is prepared by taking molasses as a raw material, and the dry basis content of the honey fermentation waste liquid is more than 40%; the starch fermentation waste liquid is prepared from starch serving as a raw material, the dry basis content of the starch fermentation waste liquid is more than 40%, and the honey fermentation waste liquid and the starch fermentation waste liquid are (5-12): (3-7).
As a preferable technical scheme of the invention, the potassium content in the honey fermentation waste liquid accounts for more than 50% of the total dry basis content of the honey fermentation waste liquid, wherein the nitrogen content accounts for more than 50% of the total dry basis content.
As a preferable technical scheme of the invention, the mass ratio of the allantoin, the bean flour and the yeast powder in the nitrogen source is (0.2-0.5) to (20-30): (3-6).
As a preferable technical scheme of the invention, the pH buffering agent is sodium citrate and potassium hydrogen phosphate according to the mass ratio of (2-5): (3-7).
As a preferred technical scheme of the invention, the biological catalytic enzyme is peptone, agarose and DNA polymerase according to the mass ratio of (10-20): (15-25): (2-4) in the presence of a catalyst.
A preparation method of a biological synergistic activator capable of improving microbial activity comprises the following steps:
(1) preparing a fermentation mixed solution: uniformly mixing the honey fermentation waste liquid and the starch fermentation waste liquid at normal temperature to prepare a fermentation mixed liquid;
(2) adding a carbon source: adding corn starch into the fermentation mixed liquid, and uniformly mixing at normal temperature to prepare primary mixed liquid;
(3) adding a nitrogen source: uniformly mixing allantoin, bean flour and yeast powder at normal temperature to prepare a nitrogen source, adding the nitrogen source into the primary mixed liquid prepared in the step (2), uniformly mixing to prepare a secondary mixed liquid;
(4) uniformly mixing sodium citrate and potassium hydrogen phosphate, crushing and grinding to obtain a pH buffering agent, adding the pH buffering agent into the secondary mixed liquid prepared in the step (3), heating the mixed liquid to 50-55 ℃, fully mixing and dissolving, and cooling to room temperature;
(5) adding steroid saponin into the solution prepared in the step (4), uniformly mixing, heating to 120-150 ℃, sterilizing, and cooling to room temperature;
(6) adding the biological catalytic enzyme into the sterilized solution obtained in the step (5), uniformly mixing to obtain the biological synergistic activator, placing the biological synergistic activator into a storage tank, and sealing and storing at the temperature of 15 +/-5 ℃.
The invention also provides a using method of the biological synergistic activator for improving the microbial activity, the synergistic activator is prepared 24-36 hours in advance and is fermented, and after fermentation, the fermented synergistic activator is added into the diluted microbial agent, so that the biological synergistic activator for treating sewage can be prepared.
Furthermore, an industrial sewage treatment tank body is additionally arranged at the opening of the industrial sewage discharge pipe, and a biological synergistic activator is added into the sewage treatment tank body.
Compared with the prior art, the invention can achieve the following beneficial effects:
1. the introduction of specific biological enzyme as catalyst can initiate and accelerate biochemical reaction by approaching orientation effect and deformation effect. The chemical chain of the substrate of the pollutants difficult to degrade can be opened, the blocked metabolic pathway is opened, various pollutants are quickly converted into micromolecular organic matters easy to degrade, and the removal rate of the biodegradation of the pollutants is improved;
2. by adding the components beneficial to the microbial activity, the living environment of the microbes in the biochemical system is obviously improved, the physiological activity and the toxicity resistance of the microbes are enhanced, and the processing capacity and the impact load resistance of the microbial system can be further improved;
3. aiming at the defect of the microbial metabolism function of the original biological system, functional microbes are introduced in an immobilization mode, so that the population and the quantity of the functional microbes of the biochemical system are greatly improved, the mass transfer speed of pollutants difficult to degrade in wastewater is greatly improved, and the processing capacity of the biochemical system is obviously improved;
4. after the biochemical system operates stably, the effect of the system can be maintained only by using a very small amount of supplementary medicament, the use of other medicaments can be reduced, the sludge production is reduced, and the comprehensive treatment cost of the system is reduced.
Detailed Description
The present invention will be further described with reference to specific embodiments for the purpose of facilitating an understanding of technical means, characteristics of creation, objectives and functions realized by the present invention, but the following embodiments are only preferred embodiments of the present invention, and are not intended to be exhaustive. Based on the embodiments in the implementation, other embodiments obtained by those skilled in the art without any creative efforts belong to the protection scope of the present invention. The experimental methods in the following examples are conventional methods unless otherwise specified, and materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
The invention provides a biological synergistic activator capable of improving microbial activity, which comprises the following components in percentage by mass: 20-40 parts of carbon source, 50-80 parts of fermentation mixed liquor, 15-30 parts of nitrogen source, 20-50 parts of steroid saponin, 2-11 parts of pH buffer and 0.5-3 parts of biological catalytic enzyme, wherein the carbon source is corn starch, the nitrogen source is a mixture consisting of allantoin, bean flour and yeast powder, and the fermentation mixed liquor is a mixture consisting of honey fermentation waste liquor and starch fermentation waste liquor.
The honey fermentation waste liquid is prepared by taking molasses as a raw material, and the dry basis content of the honey fermentation waste liquid is more than 40%; the starch fermentation waste liquid is prepared from starch serving as a raw material, the dry basis content of the starch fermentation waste liquid is more than 40%, and the honey fermentation waste liquid and the starch fermentation waste liquid are (5-12): (3-7). The potassium content in the honey fermentation waste liquid accounts for more than 50% of the total dry basis content of the honey fermentation waste liquid, and the nitrogen content accounts for more than 50% of the total dry basis content.
The mass ratio of allantoin, bean flour and yeast powder in the nitrogen source is (0.2-0.5) to (20-30): (3-6).
The pH buffering agent is sodium citrate and potassium hydrogen phosphate according to a mass ratio of (2-5): (3-7).
The biological catalytic enzyme is peptone, agarose and DNA polymerase according to the mass ratio (10-20): (15-25): (2-4) in the presence of a catalyst.
Example 1
A preparation method of a biological synergistic activator capable of improving microbial activity comprises the following steps:
(1) preparing a fermentation mixed solution: according to the mass ratio of 5:3, uniformly mixing the honey fermentation waste liquid and the starch fermentation waste liquid at normal temperature to prepare a fermentation mixed liquid;
(2) adding a carbon source: adding 20 parts by mass of corn starch into the fermentation mixed liquid, and uniformly mixing at normal temperature to prepare primary mixed liquid;
(3) adding a nitrogen source: taking the mixture with the mass ratio of 0.2: 20: 3, uniformly mixing the allantoin, the bean powder and the yeast powder at normal temperature to prepare a nitrogen source, adding 15 parts of the nitrogen source into the primary mixed solution prepared in the step (2), uniformly mixing, and preparing a secondary mixed solution;
(4) taking sodium citrate and potassium hydrogen phosphate, and mixing the components according to the weight ratio of 2: 3, grinding and grinding to obtain a pH buffering agent, adding 2 parts of the pH buffering agent into the secondary mixed solution prepared in the step (3), heating the mixed solution to 50 ℃, fully mixing and dissolving, and cooling to room temperature;
(5) adding 10 parts of steroid saponin into the solution prepared in the step (4), uniformly mixing, heating to 120 ℃, sterilizing, and cooling to room temperature;
(6) and (3) adding 0.5 part of biocatalysis enzyme into the sterilized solution obtained in the step (5), uniformly mixing to prepare a biological synergistic activator, placing the biological synergistic activator into a storage tank, and sealing and storing at the temperature of 15 +/-5 ℃.
Example 2
A preparation method of a biological synergistic activator capable of improving microbial activity comprises the following steps:
(1) preparing a fermentation mixed solution: according to the mass ratio of 7: 4, uniformly mixing the honey fermentation waste liquid and the starch fermentation waste liquid at normal temperature to prepare a fermentation mixed liquid;
(2) adding a carbon source: adding 25 parts by mass of corn starch into the fermentation mixed liquid, and uniformly mixing at normal temperature to prepare primary mixed liquid;
(3) adding a nitrogen source: taking the mass ratio of 0.25: 22: 3.5, uniformly mixing the allantoin, the bean powder and the yeast powder at normal temperature to prepare a nitrogen source, adding 18 parts of the nitrogen source into the primary mixed solution prepared in the step (2), uniformly mixing to prepare a secondary mixed solution;
(4) taking sodium citrate and potassium hydrogen phosphate, and mixing the components according to the proportion of 3: 4, grinding and grinding to obtain a pH buffering agent, adding 3 parts of the pH buffering agent into the secondary mixed solution prepared in the step (3), heating the mixed solution to 50 ℃, fully mixing and dissolving, and cooling to room temperature;
(5) adding 15 parts of steroid saponin into the solution prepared in the step (4), uniformly mixing, heating to 130 ℃, sterilizing, and cooling to room temperature;
(6) and (3) adding 1 part of biocatalysis enzyme into the sterilized solution obtained in the step (5) for uniform mixing to prepare a biological synergistic activator, placing the biological synergistic activator into a storage tank, and sealing and storing at the temperature of 15 +/-5 ℃.
Example 3
A preparation method of a biological synergistic activator capable of improving microbial activity comprises the following steps:
(1) preparing a fermentation mixed solution: according to the mass ratio of 8: 4.5 uniformly mixing the honey fermentation waste liquid and the starch fermentation waste liquid at normal temperature to prepare a fermentation mixed liquid;
(2) adding a carbon source: adding 30 parts by mass of corn starch into the fermentation mixed liquid, and uniformly mixing at normal temperature to prepare primary mixed liquid;
(3) adding a nitrogen source: taking the mixture with the mass ratio of 0.3: 24: 4, uniformly mixing the allantoin, the bean powder and the yeast powder at normal temperature to prepare a nitrogen source, adding 20 parts of the nitrogen source into the primary mixed solution prepared in the step (2), uniformly mixing, and preparing a secondary mixed solution;
(4) taking sodium citrate and potassium hydrogen phosphate, and mixing the components according to the weight ratio of 3.5: 4.5, grinding to obtain a pH buffering agent, adding 4 parts of the pH buffering agent into the secondary mixed solution prepared in the step (3), heating the mixed solution to 50 ℃, fully mixing and dissolving, and cooling to room temperature;
(5) adding 18 parts of steroid saponin into the solution prepared in the step (4), uniformly mixing, heating to 135 ℃, sterilizing, and cooling to room temperature;
(6) and (3) adding 1.5 parts of biological catalytic enzyme into the sterilized solution obtained in the step (5) for uniform mixing to prepare a biological synergistic activator, placing the biological synergistic activator into a storage tank, and sealing and storing at the temperature of 15 +/-5 ℃.
Example 4
A preparation method of a biological synergistic activator capable of improving microbial activity comprises the following steps:
(1) preparing a fermentation mixed solution: according to the mass ratio of 9: 5, uniformly mixing the honey fermentation waste liquid and the starch fermentation waste liquid at normal temperature to prepare a fermentation mixed liquid;
(2) adding a carbon source: adding 30 parts by mass of corn starch into the fermentation mixed liquid, and uniformly mixing at normal temperature to prepare primary mixed liquid;
(3) adding a nitrogen source: taking the mixture with the mass ratio of 0.3: 26: 4, uniformly mixing the allantoin, the bean powder and the yeast powder at normal temperature to prepare a nitrogen source, adding 20 parts of the nitrogen source into the primary mixed solution prepared in the step (2), uniformly mixing, and preparing a secondary mixed solution;
(4) taking sodium citrate and potassium hydrogen phosphate, and mixing the components according to the weight ratio of 4: 4.5, grinding to obtain a pH buffering agent, adding 6 parts of the pH buffering agent into the secondary mixed solution prepared in the step (3), heating the mixed solution to 50 ℃, fully mixing and dissolving, and cooling to room temperature;
(5) adding 20 parts of steroid saponin into the solution prepared in the step (4), uniformly mixing, heating to 135 ℃, sterilizing, and cooling to room temperature;
(6) and (3) adding 2 parts of biological catalytic enzyme into the sterilized solution obtained in the step (5) for uniform mixing to prepare a biological synergistic activator, placing the biological synergistic activator into a storage tank, and sealing and storing at the temperature of 15 +/-5 ℃.
Example 5
A preparation method of a biological synergistic activator capable of improving microbial activity comprises the following steps:
(1) preparing a fermentation mixed solution: according to the mass ratio of 10: 6, uniformly mixing the honey fermentation waste liquid and the starch fermentation waste liquid at normal temperature to prepare a fermentation mixed liquid;
(2) adding a carbon source: adding 35 parts by mass of corn starch into the fermentation mixed liquid, and uniformly mixing at normal temperature to prepare primary mixed liquid;
(3) adding a nitrogen source: uniformly mixing allantoin, bean flour and yeast powder in a mass ratio of 0.4:30:5 at normal temperature to prepare a nitrogen source, adding 25 parts of the nitrogen source into the primary mixed liquid prepared in the step (2), uniformly mixing, and preparing a secondary mixed liquid;
(4) taking sodium citrate and potassium hydrogen phosphate, and mixing the components according to the weight ratio of 4: 6, uniformly mixing, crushing and grinding to obtain a pH buffering agent, adding 8 parts of the pH buffering agent into the secondary mixed solution prepared in the step (3), heating the mixed solution to 50 ℃, fully mixing and dissolving, and cooling to room temperature;
(5) adding 20 parts of steroid saponin into the solution prepared in the step (4), uniformly mixing, heating to 140 ℃, sterilizing, and cooling to room temperature;
(6) and (3) adding 2.5 parts of biological catalytic enzyme into the sterilized solution obtained in the step (5) for uniform mixing to prepare a biological synergistic activator, placing the biological synergistic activator into a storage tank, and sealing and storing at the temperature of 15 +/-5 ℃.
Example 6
A preparation method of a biological synergistic activator capable of improving microbial activity comprises the following steps:
(1) preparing a fermentation mixed solution: according to the mass ratio of 12: 7, uniformly mixing the honey fermentation waste liquid and the starch fermentation waste liquid at normal temperature to prepare a fermentation mixed liquid;
(2) adding a carbon source: adding 40 parts by mass of corn starch into the fermentation mixed liquid, and uniformly mixing at normal temperature to prepare primary mixed liquid;
(3) adding a nitrogen source: taking the mixture with the mass ratio of 0.5: 30: 6, uniformly mixing allantoin, bean powder and yeast powder at normal temperature to prepare a nitrogen source, adding 30 parts of the nitrogen source into the primary mixed solution prepared in the step (2), uniformly mixing to prepare a secondary mixed solution;
(4) taking sodium citrate and potassium hydrogen phosphate, and mixing the components according to the proportion of 5: 7, grinding to obtain a pH buffering agent, adding 11 parts of the pH buffering agent into the secondary mixed solution prepared in the step (3), heating the mixed solution to 50 ℃, fully mixing and dissolving, and cooling to room temperature;
(5) adding 25 parts of steroid saponin into the solution prepared in the step (4), uniformly mixing, heating to 150 ℃, sterilizing, and cooling to room temperature;
(6) and (3) adding 3 parts of biological catalytic enzyme into the sterilized solution obtained in the step (5) for uniform mixing to prepare a biological synergistic activator, placing the biological synergistic activator into a storage tank, and sealing and storing at the temperature of 15 +/-5 ℃.
Application example
The biological synergistic activator of the embodiments 1 to 6 of the invention is adopted to carry out biological synergistic action on a sewage treatment plant of a certain chemical plant, the synergistic activator is prepared and fermented one day before the biological synergistic activator is applied, and after the fermentation, the fermented synergistic activator is added into the diluted microbial agent to prepare the biological synergistic activator for treating sewage. An industrial sewage treatment tank body is additionally arranged at the opening of the industrial sewage discharge pipe, and a biological synergistic active agent is added into the sewage treatment tank body. The daily treatment capacity of the sewage treatment plant is 10000m3The average value of COD of the inlet water in months is 3200mg/L, the average value of COD of the outlet water in months is 400mg/L before the treatment by using the biological synergistic activator, and the average value of COD of the outlet water in months after the treatment by using the biological synergistic activator is shown in the following table 1:
TABLE 1
As can be seen from table 1 above, the bio-synergistic active agent provided by the present invention has good microbial activity, can significantly improve sewage treatment efficiency, and the comparison shows that the use effect of the bio-synergistic active agent prepared in example 3 is the best.
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and the preferred embodiments of the present invention are described in the above embodiments and the description, and are not intended to limit the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (9)
1. A biological synergistic activator capable of improving microbial activity is characterized in that: comprises the following components in percentage by mass: 20-40 parts of carbon source, 50-80 parts of fermentation mixed liquor, 15-30 parts of nitrogen source, 10-25 parts of steroid saponin, 2-11 parts of pH buffer and 0.5-3 parts of biological catalytic enzyme, wherein the carbon source is corn starch, the nitrogen source is a mixture consisting of allantoin, bean flour and yeast powder, and the fermentation mixed liquor is a mixed solution consisting of honey fermentation waste liquor and starch fermentation waste liquor.
2. The bio-potentiating activator for enhancing microbial activity of claim 1, wherein: the honey fermentation waste liquid is prepared by taking molasses as a raw material, and the dry basis content of the honey fermentation waste liquid is more than 40%; the starch fermentation waste liquid is prepared by taking starch as a raw material, the dry basis content in the starch fermentation waste liquid is more than 40%, and the honey fermentation waste liquid and the starch fermentation waste liquid are (5-12) in mass ratio: (3-7).
3. The bio-potentiating activator for enhancing microbial activity of claim 2, wherein: the potassium content in the honey fermentation waste liquid accounts for more than 50% of the total dry basis content of the honey fermentation waste liquid, and the nitrogen content accounts for more than 50% of the total dry basis content.
4. The bio-potentiating activator for enhancing microbial activity of claim 1, wherein: the mass ratio of allantoin, bean flour and yeast powder in the nitrogen source is (0.2-0.5) to (20-30): (3-6).
5. The bio-potentiating activator for enhancing microbial activity of claim 1, wherein: the pH buffering agent is sodium citrate and potassium hydrogen phosphate according to a mass ratio of (2-5): (3-7).
6. The bio-potentiating activator for enhancing microbial activity of claim 1, wherein: the biological catalytic enzyme is peptone, agarose and DNA polymerase according to the mass ratio (10-20): (15-25): (2-4) in the presence of a catalyst.
7. The method for preparing a bio-synergistic activator with microbial activity improvement function according to any one of claims 1 to 6, which comprises the following steps:
(1) preparing a fermentation mixed solution: uniformly mixing the honey fermentation waste liquid and the starch fermentation waste liquid at normal temperature to prepare a fermentation mixed liquid;
(2) adding a carbon source: adding corn starch into the fermentation mixed liquid, and uniformly mixing at normal temperature to prepare primary mixed liquid;
(3) adding a nitrogen source: uniformly mixing allantoin, bean flour and yeast powder at normal temperature to prepare a nitrogen source, adding the nitrogen source into the primary mixed liquid prepared in the step (2), uniformly mixing to prepare a secondary mixed liquid;
(4) uniformly mixing sodium citrate and potassium hydrogen phosphate, crushing and grinding to obtain a pH buffering agent, adding the pH buffering agent into the secondary mixed liquid prepared in the step (3), heating the mixed liquid to 50-55 ℃, fully mixing and dissolving, and cooling to room temperature;
(5) adding steroid saponin into the solution prepared in the step (4), uniformly mixing, heating to 120-150 ℃, sterilizing, and cooling to room temperature;
(6) adding the biological catalytic enzyme into the sterilized solution obtained in the step (5), uniformly mixing to obtain the biological synergistic activator, placing the biological synergistic activator into a storage tank, and sealing and storing at the temperature of 15 +/-5 ℃.
8. The use of a bio-potentiating activator for enhancing microbial activity according to any one of claims 1 to 6, wherein: the synergistic active agent is prepared 24-36h in advance and is fermented, and after fermentation, the fermented synergistic active agent is added into the diluted microbial agent to prepare the biological synergistic active agent for treating sewage.
9. The use of the bio-potentiating activator for enhancing microbial activity of claim 8, wherein: an industrial sewage treatment tank body is additionally arranged at the opening of the industrial sewage discharge pipe, and a biological synergistic activator is added into the sewage treatment tank body.
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