CN114159350A - Hair dye using tyrosinase to catalyze artificial melanin polymerization and preparation method thereof - Google Patents
Hair dye using tyrosinase to catalyze artificial melanin polymerization and preparation method thereof Download PDFInfo
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- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 title claims abstract description 44
- 239000000118 hair dye Substances 0.000 title claims abstract description 41
- 102000003425 Tyrosinase Human genes 0.000 title claims abstract description 37
- 108060008724 Tyrosinase Proteins 0.000 title claims abstract description 37
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 238000006116 polymerization reaction Methods 0.000 title claims description 17
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 claims abstract description 69
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 57
- BXWNKGSJHAJOGX-UHFFFAOYSA-N hexadecan-1-ol Chemical compound CCCCCCCCCCCCCCCCO BXWNKGSJHAJOGX-UHFFFAOYSA-N 0.000 claims abstract description 46
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 38
- SGNZYJXNUURYCH-UHFFFAOYSA-N 5,6-dihydroxyindole Chemical compound C1=C(O)C(O)=CC2=C1NC=C2 SGNZYJXNUURYCH-UHFFFAOYSA-N 0.000 claims abstract description 26
- 229960000541 cetyl alcohol Drugs 0.000 claims abstract description 23
- 235000019864 coconut oil Nutrition 0.000 claims abstract description 23
- 239000003240 coconut oil Substances 0.000 claims abstract description 23
- GVGUFUZHNYFZLC-UHFFFAOYSA-N dodecyl benzenesulfonate;sodium Chemical compound [Na].CCCCCCCCCCCCOS(=O)(=O)C1=CC=CC=C1 GVGUFUZHNYFZLC-UHFFFAOYSA-N 0.000 claims abstract description 23
- 229940080264 sodium dodecylbenzenesulfonate Drugs 0.000 claims abstract description 23
- 102000011782 Keratins Human genes 0.000 claims abstract description 12
- 108010076876 Keratins Proteins 0.000 claims abstract description 12
- ZXVOCOLRQJZVBW-UHFFFAOYSA-N azane;ethanol Chemical compound N.CCO ZXVOCOLRQJZVBW-UHFFFAOYSA-N 0.000 claims abstract description 12
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims abstract description 12
- 235000018417 cysteine Nutrition 0.000 claims abstract description 12
- 230000007062 hydrolysis Effects 0.000 claims abstract description 9
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 9
- 102000004190 Enzymes Human genes 0.000 claims abstract description 7
- 108090000790 Enzymes Proteins 0.000 claims abstract description 7
- 238000002156 mixing Methods 0.000 claims description 12
- 238000010438 heat treatment Methods 0.000 claims description 8
- 239000003921 oil Substances 0.000 claims description 8
- 235000019198 oils Nutrition 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 claims description 2
- 239000012071 phase Substances 0.000 claims 6
- 239000008346 aqueous phase Substances 0.000 claims 2
- 238000001816 cooling Methods 0.000 claims 2
- 230000000694 effects Effects 0.000 abstract description 16
- 238000004040 coloring Methods 0.000 abstract description 7
- 238000004043 dyeing Methods 0.000 description 13
- 230000000052 comparative effect Effects 0.000 description 8
- 238000005406 washing Methods 0.000 description 5
- 239000002994 raw material Substances 0.000 description 4
- 238000001035 drying Methods 0.000 description 3
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 239000003995 emulsifying agent Substances 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 239000003906 humectant Substances 0.000 description 2
- 230000001590 oxidative effect Effects 0.000 description 2
- 239000001024 permanent hair color Substances 0.000 description 2
- 239000004094 surface-active agent Substances 0.000 description 2
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 2
- GEYOCULIXLDCMW-UHFFFAOYSA-N 1,2-phenylenediamine Chemical compound NC1=CC=CC=C1N GEYOCULIXLDCMW-UHFFFAOYSA-N 0.000 description 1
- 201000004384 Alopecia Diseases 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 231100000360 alopecia Toxicity 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 230000037308 hair color Effects 0.000 description 1
- 230000003700 hair damage Effects 0.000 description 1
- 239000003999 initiator Substances 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000002688 persistence Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000033764 rhythmic process Effects 0.000 description 1
- 239000002453 shampoo Substances 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/342—Alcohols having more than seven atoms in an unbroken chain
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
- A61K8/447—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof containing sulfur
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4906—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom
- A61K8/4913—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom having five membered rings, e.g. pyrrolidone carboxylic acid
- A61K8/492—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom having five membered rings, e.g. pyrrolidone carboxylic acid having condensed rings, e.g. indol
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/66—Enzymes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
- A61Q5/10—Preparations for permanently dyeing the hair
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/42—Colour properties
- A61K2800/43—Pigments; Dyes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/88—Two- or multipart kits
- A61K2800/884—Sequential application
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- Veterinary Medicine (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Emergency Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a hair dye utilizing tyrosinase to catalyze artificial melanin to polymerize and a preparation method thereof, wherein the hair dye comprises a first agent and a second agent; the first agent consists of the following components: 5, 6-dihydroxyindole: 0.5 to 3 percent; coconut oil: 5% -10%; cetyl alcohol: 5% -10%; sodium dodecylbenzenesulfonate: 0.2 to 1.5 percent; propylene glycol: 4% -9%; cysteine: 1% -3%; hydrolysis of keratin: 1 percent; a proper amount of ethanol ammonium is used for adjusting the pH value to 9-10; the balance of water; the second agent consists of the following components: tyrosinase, and (c) an enzyme: 0.1 to 0.5 percent; coconut oil: 5% -10%; cetyl alcohol: 5% -10%; propylene glycol: 4% -9%; sodium dodecylbenzenesulfonate: 0.2 to 1.5 percent; the balance of water. The hair dye can obviously improve the coloring effect and the color fixing effect of the hair dye, and is milder and safer.
Description
Technical Field
The invention belongs to the field of daily chemicals, and particularly relates to a hair dye utilizing tyrosinase to catalyze artificial melanin polymerization and a preparation method thereof.
Background
China will enter an aging society in the 21 st century, and with the gradual increase of people in the white hair class, the market demand of hair dyeing products is gradually increased. In addition, the rhythm of life is fast, the work is tense in the present society, white hair and alopecia caused by the tension and busy are increased obviously, and the hair dyeing product brings a huge market. However, most of the permanent hair dyes on the market currently have potential safety problems, and the carcinogenic and harmful components (such as phenylenediamine, hydrogen peroxide and the like) contained in the permanent hair dyes are more and more concerned.
Melanin in hair is formed primarily by the reaction of tyrosine and tyrosinase within the color body (immature melanin). Tyrosine is catalyzed and oxidized to form active intermediate 5, 6-dihydroxyindole, and then further oxidized to form melanin. Due to the activity of the 5, 6-dihydroxyindole intermediate, the intermediate is easy to oxidize and self-polymerize under the conditions of alkalinity and air, and does not need the stimulation of an initiator, so that the 5, 6-dihydroxyindole melanin hair dye is safer for human bodies. However, although the 5, 6-dihydroxyindole intermediate is sufficiently active, it is still difficult to oxidize and blacken the hair naturally in air in a short time, so that the color fixing effect is poor.
Disclosure of Invention
The invention aims to provide a hair dye utilizing tyrosinase to catalyze artificial melanin polymerization and a preparation method thereof, wherein the hair dye utilizes tyrosinase to catalyze 5, 6-dihydroxyindole to perform rapid oxidative polymerization to form melanin, can remarkably improve the coloring effect and the color fixing effect of the hair dye, and is milder and safer.
The technical scheme of the invention is as follows:
a hair dye which catalyzes the polymerization of artificial melanin by using tyrosinase and comprises a first agent and a second agent;
the first agent consists of the following components in percentage by mass:
5, 6-dihydroxyindole: 0.5 to 3 percent; coconut oil: 5% -10%; cetyl alcohol: 5% -10%; sodium dodecylbenzenesulfonate: 0.2 to 1.5 percent; propylene glycol: 4% -9%; cysteine: 1% -3%; hydrolysis of keratin: 1 percent; a proper amount of ethanol ammonium is used for adjusting the pH value to 9-10; the balance of water;
the second agent comprises the following components in percentage by mass:
tyrosinase, and (c) an enzyme: 0.1 to 0.5 percent; coconut oil: 5% -10%; cetyl alcohol: 5% -10%; propylene glycol: 4% -9%; sodium dodecylbenzenesulfonate: 0.2 to 1.5 percent; the balance of water.
Preferably, the mass percentage of 5, 6-dihydroxyindole in the first agent is 3%.
Preferably, the mass percentage of the tyrosinase in the second agent is 0.2-0.5%.
More preferably, the mass percent of the tyrosinase in the second agent is 0.3-0.5%.
Preferably, the second agent contains tyrosinase 0.4-0.5 wt%.
In some embodiments, the ratio of the amount of the first agent to the amount of the second agent is 1.5: 1-1: 1.5, preferably 1: 1.
in the hair dye, 5, 6-dihydroxyindole is a coloring agent; coconut oil is used as an emulsifier; cetyl alcohol and sodium dodecyl benzene sulfonate are used as surfactants; propylene glycol is used as humectant; cysteine is an antioxidant and can improve the activity of tyrosinase; hydrolyzed keratin is a humectant and can repair hair; ethanol ammonium as pH regulator; tyrosinase is used as catalyst.
The invention uses tyrosinase to promote the oxidative polymerization of 5, 6-dihydroxyindole, thereby promoting the generation of melanin on hair, remarkably improving the coloring effect of the hair dye, reducing the hair damage and being milder and safer.
The emulsifier, the moisture retention agent, the surfactant and the like in the invention are all conventional components in the hair dye, and the adopted dosage is also conventional, and the conventional components have no obvious influence on the achievement of the purpose of the invention. The hair dye disclosed by the invention has the key component capable of achieving the black dyeing effect, namely the 5, 6-dihydroxyindole, and the tyrosinase is adopted to promote the polymerization of the 5, 6-dihydroxyindole, so that the hair dye has more excellent coloring effect and color fixing effect, and is milder and safer.
The preparation method of the hair dye comprises the following steps of preparing a first agent:
(1-1) mixing and heating coconut oil and cetyl alcohol to 50-80 ℃ to obtain an oil phase;
(1-2) mixing and heating sodium dodecyl benzene sulfonate, propylene glycol, cysteine, hydrolyzed keratin and water to 50-80 ℃ to obtain a water phase;
(1-3) adding the water phase into the oil phase, stirring for 2-8 min, and then reducing the temperature to room temperature;
(1-4) adding ethanol ammonium to adjust the pH value to 9-10, adding 5, 6-dihydroxyindole, and mixing to obtain a first agent;
the amount of each raw material is referred to the above mass percentage.
The preparation method of the hair dye comprises the preparation of a second agent, and comprises the following steps:
(2-1) mixing and heating coconut oil and cetyl alcohol to 50-80 ℃ to obtain an oil phase;
(2-2) mixing and heating sodium dodecyl benzene sulfonate, propylene glycol and water to 50-80 ℃ to obtain a water phase;
(2-3) adding the water phase into the oil phase, stirring for 2-8 min, and then reducing the temperature to room temperature;
(2-4) adding tyrosinase and mixing to obtain a second agent;
the amount of each raw material is referred to the above mass percentage.
The use method of the hair dye comprises the following steps:
cleaning the hair, smearing the first agent on the hair, and staying in the air for 20-40 min; rubbing the hair for 10-20 min with a second agent, washing with clear water and drying to finish the hair dyeing process; wherein, the dosage ratio of the first agent and the second agent is compared with the dosage ratio of the conventional two-agent hair dye, and the dosage ratio is generally 1.5: 1-1: 1.5, preferably according to 1: 1.
compared with the prior art, the invention has the following characteristics and beneficial effects:
1. the non-toxic and safe 5, 6-dihydroxyindole is used as a melanin intermediate of the hair dye, so that the hair dye is more environment-friendly.
2. The tyrosinase is used for catalyzing the rapid polymerization of the 5, 6-dihydroxyindole, so that the coloring can be rapidly realized on the premise of ensuring the mildness and the safety, the coloring effect is obvious, and the coloring is firm.
Detailed Description
The technical solution and the technical effect of the present invention will be further explained with reference to the following embodiments. The examples are only for illustrative purposes and are not intended to limit the scope of the present invention. The technical solution of the present invention is further described below with reference to specific examples.
In the following examples and comparative examples, the raw materials used were all purchased directly from the market, wherein 5, 6-dihydroxyindole was a product of Sichuan melanine Polymer materials Co., Ltd., purity was 97%; the other raw materials are also common products in the market.
Example 1
The first agent of the hair dye of the embodiment comprises the following components: 5, 6-dihydroxyindole: 3%, coconut oil: 5%, cetyl alcohol: 5%, sodium dodecylbenzenesulfonate: 0.5%, propylene glycol: 5%, cysteine: 3%, hydrolysis of keratin: 1 percent of ammonium ethoxide, and is used for adjusting the pH value of the first agent to 9.5, and the balance of water, wherein the mass percent of each component is 100 percent. The second agent comprises the following components: tyrosinase, and (c) an enzyme: 0.1%, coconut oil: 5%, cetyl alcohol: 5%, propylene glycol: 5%, sodium dodecylbenzenesulfonate: 0.5 percent of water and the balance of water, wherein the mass percent of all the components is 100 percent.
Example 2
The first agent of the hair dye of the embodiment comprises the following components: 5, 6-dihydroxyindole: 3%, coconut oil: 5%, cetyl alcohol: 5%, sodium dodecylbenzenesulfonate: 0.5%, propylene glycol: 5%, cysteine: 3%, hydrolysis of keratin: 1 percent of ammonium ethoxide, and is used for adjusting the pH value of the first agent to 9.5, and the balance of water, wherein the mass percent of each component is 100 percent. The second agent comprises the following components: tyrosinase, and (c) an enzyme: 0.2%, coconut oil: 5%, cetyl alcohol: 5%, propylene glycol: 5%, sodium dodecylbenzenesulfonate: 0.5 percent of water and the balance of water, wherein the mass percent of all the components is 100 percent.
Example 3
The first agent of the hair dye of the embodiment comprises the following components: 5, 6-dihydroxyindole: 3%, coconut oil: 5%, cetyl alcohol: 5%, sodium dodecylbenzenesulfonate: 0.5%, propylene glycol: 5%, cysteine: 3%, hydrolysis of keratin: 1 percent of ammonium ethoxide, and is used for adjusting the pH value of the first agent to 9.5, and the balance of water, wherein the mass percent of each component is 100 percent. The second agent comprises the following components: tyrosinase, and (c) an enzyme: 0.3%, coconut oil: 5%, cetyl alcohol: 5%, propylene glycol: 5%, sodium dodecylbenzenesulfonate: 0.5 percent of water and the balance of water, wherein the mass percent of all the components is 100 percent.
Example 4
The first agent of the hair dye of the embodiment comprises the following components: 5, 6-dihydroxyindole: 3%, coconut oil: 5%, cetyl alcohol: 5%, sodium dodecylbenzenesulfonate: 0.5%, propylene glycol: 5%, cysteine: 3%, hydrolysis of keratin: 1 percent of ammonium ethoxide, and is used for adjusting the pH value of the first agent to 9.5, and the balance of water, wherein the mass percent of each component is 100 percent. The second agent comprises the following components: tyrosinase 0.4%, coconut oil: 5%, cetyl alcohol: 5%, propylene glycol: 5%, sodium dodecylbenzenesulfonate: 0.5 percent of water and the balance of water, wherein the mass percent of all the components is 100 percent.
Example 5
The first agent of the hair dye of the embodiment comprises the following components: 5, 6-dihydroxyindole: 3%, coconut oil: 5%, cetyl alcohol: 5%, sodium dodecylbenzenesulfonate: 0.5%, propylene glycol: 5%, cysteine: 3%, hydrolysis of keratin: 1 percent of ammonium ethoxide, and is used for adjusting the pH value of the first agent to 9.5, and the balance of water, wherein the mass percent of each component is 100 percent. The second agent comprises the following components: tyrosinase, and (c) an enzyme: 0.5%, coconut oil: 5%, cetyl alcohol: 5%, propylene glycol: 5%, sodium dodecylbenzenesulfonate: 0.5 percent of water and the balance of water, wherein the mass percent of all the components is 100 percent.
Comparative example 1
The first agent of the hair dye of this comparative example was composed of: 5, 6-dihydroxyindole: 3%, coconut oil: 5%, cetyl alcohol: 5%, sodium dodecylbenzenesulfonate: 0.5%, propylene glycol: 5%, cysteine: 3%, hydrolysis of keratin: 1 percent of ammonium ethoxide, and is used for adjusting the pH value of the first agent to 9.5, and the balance of water, wherein the mass percent of each component is 100 percent. The second agent comprises the following components: coconut oil: 5%, cetyl alcohol: 5%, propylene glycol: 5%, sodium dodecylbenzenesulfonate: 0.5 percent of water and the balance of water, wherein the mass percent of all the components is 100 percent.
The compositions of examples 1 to 5 and comparative example 1 are shown in Table 1.
TABLE 1 Components of examples 1-5 and comparative example 1
The effect of the hair dye of the embodiments 1 to 5 is evaluated by the following method: cleaning white hair with L value of 66.2 and chroma of 10 with shampoo, uniformly applying the first preparation on hair, and standing in air for 30 min; rubbing and washing the hair for 15min by using a second agent, and then washing and drying the hair by using clean water. And evaluating the hair dyeing blackness and the fixation durability of the hair.
The evaluation method of the hair dyeing blackness comprises the following steps: and testing the blackness for 3 times by using a color difference meter, and taking an average value. The blackness L value range of normal black hair is 16-18, and the smaller the L value is, the blacker the hair color is. The evaluation method of the fixation durability comprises the following steps: and repeatedly washing and drying the hair after the hair dyeing is finished, wherein one-time washing is one cycle, the times of reducing the hair chroma by one degree are recorded as the times of water resistance, and the durability of the hair dyeing effect is evaluated through the times of water resistance.
The dyeing blackness and fixation durability of the hair dye of the example 1-5 and the comparative example 1 are shown in Table 2. As can be seen from the table 2, compared with the comparative example 1, the hair dyeing blackness and the fixation durability of the hair dyes in the examples 1 to 5 are obviously improved. The trace tyrosinase can efficiently and mildly catalyze the rapid polymerization of 5, 6-dihydroxyindole molecules to form melanin, and the melanin is obvious in black dyeing effect, high in fixation durability after dyeing and not easy to wash.
TABLE 2 Hair dyeing blackness and fixation durability of Hair dyes
| Blackness of hair dyed | Fastness persistence | |
| Example 1 | 23.5 | 39 |
| Example 2 | 21.6 | 42 |
| Example 3 | 19.7 | 41 |
| Example 4 | 18.9 | 42 |
| Example 5 | 17.4 | 43 |
| Comparative example 1 | 26.6 | 28 |
The foregoing are merely exemplary embodiments of the present invention, which enable those skilled in the art to understand or practice the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (8)
1. The hair dye using tyrosinase to catalyze the artificial melanin polymerization is characterized in that:
comprises a first agent and a second agent;
the first agent consists of the following components in percentage by mass:
5, 6-dihydroxyindole: 0.5 to 3 percent; coconut oil: 5% -10%; cetyl alcohol: 5% -10%; sodium dodecylbenzenesulfonate: 0.2 to 1.5 percent; propylene glycol: 4% -9%; cysteine: 1% -3%; hydrolysis of keratin: 1 percent; a proper amount of ethanol ammonium is used for adjusting the pH value to 9-10; the balance of water;
the second agent comprises the following components in percentage by mass:
tyrosinase, and (c) an enzyme: 0.1 to 0.5 percent; coconut oil: 5% -10%; cetyl alcohol: 5% -10%; propylene glycol: 4% -9%; sodium dodecylbenzenesulfonate: 0.2 to 1.5 percent; the balance of water.
2. The hair dye using tyrosinase catalyzed artificial melanin polymerization as claimed in claim 1, wherein:
the mass percentage of the 5, 6-dihydroxyindole in the first agent is 3%.
3. The hair dye using tyrosinase catalyzed artificial melanin polymerization as claimed in claim 1, wherein:
the mass percentage of the tyrosinase in the second agent is 0.2-0.5%.
4. The hair dye using tyrosinase catalyzed artificial melanin polymerization as claimed in claim 1, wherein:
the mass percentage of the tyrosinase in the second agent is 0.3-0.5%.
5. The hair dye using tyrosinase catalyzed artificial melanin polymerization as claimed in claim 1, wherein:
the mass percentage of the tyrosinase in the second agent is 0.4-0.5%.
6. The hair dye using tyrosinase catalyzed artificial melanin polymerization as claimed in claim 1, wherein:
the dosage ratio of the first dose to the second dose is 1.5: 1-1: 1.5.
7. the hair dye using tyrosinase catalyzed artificial melanin polymerization as claimed in claim 6, wherein:
the dosage ratio of the first dose to the second dose is 1: 1.
8. the method for preparing a hair dye according to any one of claims 1 to 5, which comprises: comprises the preparation of a first agent and a second agent;
the preparation of the first agent comprises the steps of:
(1-1) mixing and heating coconut oil and cetyl alcohol to 50-80 ℃ to obtain an oil phase;
(1-2) mixing and heating sodium dodecyl benzene sulfonate, propylene glycol, cysteine, hydrolyzed keratin and water to 50-80 ℃ to obtain a water phase;
(1-3) adding the aqueous phase to the oil phase, stirring, and then cooling to room temperature;
(1-4) adding ethanol ammonium to adjust the pH value to 9-10, adding 5, 6-dihydroxyindole, and mixing to obtain a first agent;
the preparation of the second agent comprises the steps of:
(2-1) mixing and heating coconut oil and cetyl alcohol to 50-80 ℃ to obtain an oil phase;
(2-2) mixing and heating sodium dodecyl benzene sulfonate, propylene glycol and water to 50-80 ℃ to obtain a water phase;
(2-3) adding the aqueous phase to the oil phase, stirring, and then cooling to room temperature;
(2-4) adding tyrosinase and mixing to obtain a second agent.
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| CN202111539459.5A CN114159350A (en) | 2021-12-16 | 2021-12-16 | Hair dye using tyrosinase to catalyze artificial melanin polymerization and preparation method thereof |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0348612A (en) * | 1989-07-18 | 1991-03-01 | Nikka Chem Co Ltd | Hair dyeing composition |
| CN102695495A (en) * | 2009-11-13 | 2012-09-26 | 华纳贝比克研究所绿色化学有限公司 | Coloring composition containing an aromatic compound and tyrosinase |
| CN106109258A (en) * | 2016-07-06 | 2016-11-16 | 南京普瑞彩生物科技有限公司 | Melanin precursor material hair dye and using method thereof |
| CN111920696A (en) * | 2020-09-14 | 2020-11-13 | 四川大学 | Artificial melanin hair-perming and dyeing agent and preparation and hair-perming and dyeing integrated use method thereof |
-
2021
- 2021-12-16 CN CN202111539459.5A patent/CN114159350A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0348612A (en) * | 1989-07-18 | 1991-03-01 | Nikka Chem Co Ltd | Hair dyeing composition |
| CN102695495A (en) * | 2009-11-13 | 2012-09-26 | 华纳贝比克研究所绿色化学有限公司 | Coloring composition containing an aromatic compound and tyrosinase |
| CN106109258A (en) * | 2016-07-06 | 2016-11-16 | 南京普瑞彩生物科技有限公司 | Melanin precursor material hair dye and using method thereof |
| CN111920696A (en) * | 2020-09-14 | 2020-11-13 | 四川大学 | Artificial melanin hair-perming and dyeing agent and preparation and hair-perming and dyeing integrated use method thereof |
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