CN114152707A - Method for identifying traditional Chinese medicine poria with hostwood - Google Patents
Method for identifying traditional Chinese medicine poria with hostwood Download PDFInfo
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- CN114152707A CN114152707A CN202111276398.8A CN202111276398A CN114152707A CN 114152707 A CN114152707 A CN 114152707A CN 202111276398 A CN202111276398 A CN 202111276398A CN 114152707 A CN114152707 A CN 114152707A
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- 241001619461 Poria <basidiomycete fungus> Species 0.000 title claims abstract description 39
- 238000000034 method Methods 0.000 title claims abstract description 14
- 239000003814 drug Substances 0.000 title claims abstract description 13
- 239000000463 material Substances 0.000 claims abstract description 39
- 235000008599 Poria cocos Nutrition 0.000 claims abstract description 30
- 239000000243 solution Substances 0.000 claims abstract description 19
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims abstract description 12
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000011259 mixed solution Substances 0.000 claims abstract description 6
- 238000012360 testing method Methods 0.000 claims abstract description 6
- 238000004809 thin layer chromatography Methods 0.000 claims abstract description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 4
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 4
- 238000001704 evaporation Methods 0.000 claims abstract description 4
- 239000000706 filtrate Substances 0.000 claims abstract description 4
- 238000001914 filtration Methods 0.000 claims abstract description 4
- 238000010438 heat treatment Methods 0.000 claims abstract description 4
- 239000000843 powder Substances 0.000 claims abstract description 4
- 238000007789 sealing Methods 0.000 claims abstract description 4
- 238000005507 spraying Methods 0.000 claims abstract description 4
- 238000001035 drying Methods 0.000 claims abstract description 3
- 241001619444 Wolfiporia cocos Species 0.000 claims description 22
- 239000000523 sample Substances 0.000 claims description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 6
- MWOOGOJBHIARFG-UHFFFAOYSA-N vanillin Chemical compound COC1=CC(C=O)=CC=C1O MWOOGOJBHIARFG-UHFFFAOYSA-N 0.000 claims description 6
- FGQOOHJZONJGDT-UHFFFAOYSA-N vanillin Natural products COC1=CC(O)=CC(C=O)=C1 FGQOOHJZONJGDT-UHFFFAOYSA-N 0.000 claims description 6
- 235000012141 vanillin Nutrition 0.000 claims description 6
- 239000012925 reference material Substances 0.000 claims description 5
- ROAYSRAUMPWBQX-UHFFFAOYSA-N ethanol;sulfuric acid Chemical compound CCO.OS(O)(=O)=O ROAYSRAUMPWBQX-UHFFFAOYSA-N 0.000 claims description 3
- 239000012488 sample solution Substances 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 2
- 238000012545 processing Methods 0.000 claims description 2
- 238000011160 research Methods 0.000 abstract description 5
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 241000282461 Canis lupus Species 0.000 abstract description 2
- 238000002360 preparation method Methods 0.000 abstract description 2
- 244000197580 Poria cocos Species 0.000 abstract 8
- 235000001188 Peltandra virginica Nutrition 0.000 abstract 1
- 239000012085 test solution Substances 0.000 abstract 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 3
- 235000011613 Pinus brutia Nutrition 0.000 description 3
- 241000018646 Pinus brutia Species 0.000 description 3
- 206010033557 Palpitations Diseases 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 206010006895 Cachexia Diseases 0.000 description 1
- 206010010904 Convulsion Diseases 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 241000222341 Polyporaceae Species 0.000 description 1
- 241001558929 Sclerotium <basidiomycota> Species 0.000 description 1
- 208000012886 Vertigo Diseases 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- 230000001914 calming effect Effects 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000036461 convulsion Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 208000026500 emaciation Diseases 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 230000002936 tranquilizing effect Effects 0.000 description 1
- 238000002137 ultrasound extraction Methods 0.000 description 1
- 231100000889 vertigo Toxicity 0.000 description 1
Images
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses an identification method of traditional Chinese medicine poria with hostwood. The method comprises the following steps: smashing poria cocos wolf to be detected for later use; taking 1g of the prepared powder, adding 50ml of diethyl ether, sealing, ultrasonically extracting for 10 minutes, filtering, evaporating filtrate to dryness, and adding methanol lml into residues to dissolve the residues to obtain a test solution; taking another reference medicinal material Poria 1g to prepare a reference medicinal material solution; performing thin layer chromatography test, namely sucking 5 of the two solutions respectively, dropping the two solutions on the same silica gel G thin layer plate respectively, developing the two solutions by taking toluene-ethyl acetate-formic acid as a developing agent according to the mass ratio of 20:5:0.5, taking out the solutions, drying the solutions in the air, spraying a 2% vanillin-sulfuric acid-ethanol mixed solution, and heating the solutions at 105 ℃ until spots are clearly displayed; and displaying whether the main spots have the same color or not at the positions corresponding to the chromatogram of the control medicinal material tuckahoe. The method is beneficial to qualitative identification of the poria cocos medicinal material, can distinguish the poria cocos medicinal material from the poria cocos medicinal material, can effectively determine the specificity of the poria cocos and the poria cocos in the research of the poria cocos decoction preparation, and lays a foundation for the research of the effective components of the subsequent decoction.
Description
Technical Field
The invention belongs to the technical field of traditional Chinese medicine identification, and relates to an identification method of traditional Chinese medicine poria with hostwood.
Background
Poria cum radix et rhizoma Rhei decoction is used for tranquilizing mind, relieving heart-qi deficiency, deficiency and palpitation, daily vexation and cry, and infantile diseases such as emaciation and multiple convulsion. The Poria decoction mainly comprises traditional Chinese medicine Poria.
Poria is part of sclerotium of Poria cocos (Schw.) wolf of Polyporaceae with pine root, and the original species is lower plant, which is fungus parasitized on pine tree. The medicinal part is white part of dry karyophyte core body with pine root. Sweet in nature and taste, bland and mild. Has effects in eliminating dampness, invigorating spleen, and calming heart. Can be used for treating phlegm retention, edema, dysuresia, diarrhea, palpitation, and vertigo. Distributed around the world.
The existing poria cocos medicinal material has no pharmacopoeia standard, and the existing effective standard is identified by using poria cocos as a reference medicinal material, so that the specificity of the poria cocos cannot be effectively embodied, and the poria cocos medicinal material cannot be distinguished. Therefore, an identification method for the poria with hostwood needs to be established to facilitate the utilization of the traditional Chinese medicine material poria with hostwood and the development of related applications.
Disclosure of Invention
The invention discloses an identification method of traditional Chinese medicine poria with hostwood according to the defects of the prior art. The invention aims to qualitatively identify the poria with hostwood medicinal material by taking the poria with hostwood as a reference medicinal material so as to distinguish poria from poria with hostwood, and provides a quick and accurate identification method of the traditional Chinese medicinal material poria with hostwood.
The invention is realized by the following technical scheme:
the identification method of the traditional Chinese medicine material poria with hostwood is characterized by comprising the following steps:
s1, smashing poria with hostwood to be detected for later use;
s2, taking 1g of the powder prepared in the S1, adding 50ml of diethyl ether, sealing, ultrasonically extracting for 10 minutes, filtering, evaporating filtrate to dryness, and adding methanol lml into residues to dissolve the residues to obtain a sample solution;
s3, taking another reference material Poria with 1g, and synchronously processing S1 and S2 to obtain a reference material solution;
s4, performing thin-layer chromatography test, namely sucking 5 of the two solutions respectively, respectively dropping the two solutions on the same silica gel G thin-layer plate, developing by taking toluene-ethyl acetate-formic acid with the mass ratio of 20:5:0.5 as a developing agent, taking out, drying in the air, spraying 2 wt% of vanillin, sulfuric acid and ethanol mixed solution, and heating at 105 ℃ until spots are clear;
s5, observing the positions of the chromatogram of the Poria cocos trial product corresponding to the chromatogram of the control material Poria cocos, and showing whether the main spots have the same color.
The vanillin sulfuric acid-ethanol mixed solution is prepared by mixing vanillin sulfuric acid and ethanol in a mass ratio of 4: 1.
The invention has the following beneficial effects: the invention is beneficial to the qualitative identification of the poria cocos medicinal material and can distinguish the poria cocos medicinal material from the poria cocos medicinal material. In the research of the poria decoction preparation, the specificity of the poria and the poria can be effectively determined, and the research is based on the subsequent research of the effective components of the decoction.
Drawings
FIG. 1 is a thin layer identification chart of Poria cum radix et rhizoma Rhei.
FIG. 2 is a thin layer identification chart.
In the figure, the blobs are, from left to right: poria cocos contrast medicinal material Y, poria cocos medicinal material sample 1, poria cocos medicinal material sample 2, poria cocos medicinal material sample 3, poria cocos medicinal material sample 4 and poria cocos medicinal material sample 5.
Detailed Description
The present invention is further described below in conjunction with the following detailed description, which is intended to further illustrate the principles of the invention and is not intended to limit the invention in any way, but is equivalent or analogous to the present invention without departing from its scope.
Examples
The identification method of the traditional Chinese medicine poria with hostwood aims at detecting five batches of samples of the traditional Chinese medicine poria with hostwood by thin-layer chromatography.
The detection method comprises the following steps:
1. smashing five batches of medicinal materials to be detected with poria cocos for later use;
2. taking 1g of the powder prepared in the step 1, adding 50ml of diethyl ether and sealing, carrying out ultrasonic extraction for 10 minutes, filtering, evaporating filtrate to dryness, and adding methanol lml into residues to dissolve the residues to obtain a sample solution;
3. taking 1g of reference medicinal material poria with hostwood, and preparing reference medicinal material solution in the same steps 1 and 2;
4. performing thin layer chromatography test, sucking 5 μ l of each solution, respectively dropping on the same silica gel G thin layer plate, developing with toluene-ethyl acetate-formic acid as developing agent at mass ratio of 20:5:0.5, taking out, air drying, spraying 2 wt% vanillin sulfuric acid-ethanol mixed solution, heating at 105 deg.C until spots are clearly displayed;
5. as can be seen from FIG. 1, the main spots with the same color appear at the corresponding positions of the Poria chromatogram of the five test samples and the control drug.
FIG. 1 is a thin layer identification diagram of Poria with Chinese medicinal materials, wherein Poria contrast medicinal material Y, Poria medicinal material sample 1, Poria medicinal material sample 2, Poria medicinal material sample 3, Poria medicinal material sample 4, and Poria medicinal material sample 5 are sequentially arranged from left to right.
The atlas can obviously show that the poria cocos medicinal material to be detected and the poria cocos contrast medicinal material show the same bluish purple spots at the same position, so the method can be used for qualitative identification of the poria cocos medicinal material.
Claims (2)
1. The identification method of the traditional Chinese medicine material poria with hostwood is characterized by comprising the following steps:
s1, smashing poria with hostwood to be tested for later use;
s2, taking 1g of the powder prepared in the S1, adding 50ml of diethyl ether, sealing, ultrasonically extracting for 10 minutes, filtering, evaporating filtrate to dryness, and adding 1ml of methanol into residues to dissolve the residues to obtain a sample solution;
s3, taking another reference material Poria with 1g, and synchronously processing S1 and S2 to obtain a reference material solution;
s4, performing thin-layer chromatography test, namely sucking 5 of the two solutions respectively, respectively dropping the two solutions on the same silica gel G thin-layer plate, developing the two solutions by taking toluene-ethyl acetate-formic acid as a developing agent according to the mass ratio of 20:5:0.5, taking out the solutions, drying the solutions in the air, spraying a 2% vanillin-sulfuric acid-ethanol mixed solution, and heating the solutions at 105 ℃ until spots are clear;
s5, observing the positions corresponding to the Poria cocos chromatogram of the reference material in the Poria cocos chromatogram of the test sample, and displaying whether the main spots have the same color.
2. The method for identifying traditional Chinese medicinal material poria with hostwood as claimed in claim 1, characterized in that: the vanillin sulfuric acid-ethanol mixed solution is prepared by mixing vanillin sulfuric acid and ethanol in a mass ratio of 4: 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111276398.8A CN114152707A (en) | 2021-10-29 | 2021-10-29 | Method for identifying traditional Chinese medicine poria with hostwood |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111276398.8A CN114152707A (en) | 2021-10-29 | 2021-10-29 | Method for identifying traditional Chinese medicine poria with hostwood |
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| Publication Number | Publication Date |
|---|---|
| CN114152707A true CN114152707A (en) | 2022-03-08 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111276398.8A Pending CN114152707A (en) | 2021-10-29 | 2021-10-29 | Method for identifying traditional Chinese medicine poria with hostwood |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106237249A (en) * | 2016-08-31 | 2016-12-21 | 卞永兴 | A kind of Chinese medicinal capsule for treating Women’s climacteric insomnia |
| CN113181123A (en) * | 2021-05-12 | 2021-07-30 | 湖北中医药大学 | Anmian granules and preparation method thereof |
-
2021
- 2021-10-29 CN CN202111276398.8A patent/CN114152707A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106237249A (en) * | 2016-08-31 | 2016-12-21 | 卞永兴 | A kind of Chinese medicinal capsule for treating Women’s climacteric insomnia |
| CN113181123A (en) * | 2021-05-12 | 2021-07-30 | 湖北中医药大学 | Anmian granules and preparation method thereof |
Non-Patent Citations (4)
| Title |
|---|
| 吴智高;: "茯苓及伪品茯苓鉴别", 海峡药学, no. 03, 15 March 2010 (2010-03-15) * |
| 文鑫鑫: "茯神薄层鉴别方法研究", 山东化工, vol. 50, 8 July 2021 (2021-07-08), pages 100 * |
| 河北省食品药品监督管理局: "河北省中药材标准", 31 December 2018, 河北科学技术出版社, pages: 299 * |
| 郭胜伟: "桑茯益肾汤中桑螵蛸茯神的薄层色谱鉴别", 时珍国医国药, no. 06, 30 June 2003 (2003-06-30) * |
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