CN114136961B - Rapid detection kit and detection method for pesticide carbendazim in fruit and vegetable products - Google Patents
Rapid detection kit and detection method for pesticide carbendazim in fruit and vegetable products Download PDFInfo
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- CN114136961B CN114136961B CN202111457195.9A CN202111457195A CN114136961B CN 114136961 B CN114136961 B CN 114136961B CN 202111457195 A CN202111457195 A CN 202111457195A CN 114136961 B CN114136961 B CN 114136961B
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- carbendazim
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- fruit
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- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 title claims abstract description 60
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 title claims abstract description 54
- 239000006013 carbendazim Substances 0.000 title claims abstract description 54
- 238000001514 detection method Methods 0.000 title claims abstract description 47
- 235000012055 fruits and vegetables Nutrition 0.000 title claims abstract description 17
- 239000000575 pesticide Substances 0.000 title claims abstract description 10
- 238000006243 chemical reaction Methods 0.000 claims abstract description 28
- 240000001592 Amaranthus caudatus Species 0.000 claims abstract description 19
- 235000009328 Amaranthus caudatus Nutrition 0.000 claims abstract description 19
- 235000012735 amaranth Nutrition 0.000 claims abstract description 19
- 239000004178 amaranth Substances 0.000 claims abstract description 19
- 239000012086 standard solution Substances 0.000 claims abstract description 10
- OPQARKPSCNTWTJ-UHFFFAOYSA-L copper(ii) acetate Chemical compound [Cu+2].CC([O-])=O.CC([O-])=O OPQARKPSCNTWTJ-UHFFFAOYSA-L 0.000 claims abstract description 8
- 239000000243 solution Substances 0.000 claims description 30
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 18
- 238000000034 method Methods 0.000 claims description 8
- 230000000007 visual effect Effects 0.000 claims description 7
- ZOIORXHNWRGPMV-UHFFFAOYSA-N acetic acid;zinc Chemical compound [Zn].CC(O)=O.CC(O)=O ZOIORXHNWRGPMV-UHFFFAOYSA-N 0.000 claims description 5
- -1 polyethylene Polymers 0.000 claims description 5
- 239000004246 zinc acetate Substances 0.000 claims description 5
- 239000003153 chemical reaction reagent Substances 0.000 claims description 3
- 238000000605 extraction Methods 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 239000004698 Polyethylene Substances 0.000 claims description 2
- 229920000573 polyethylene Polymers 0.000 claims description 2
- VWTSXINFCUODBJ-UHFFFAOYSA-L zinc methanol diacetate Chemical compound [Zn++].CO.CC([O-])=O.CC([O-])=O VWTSXINFCUODBJ-UHFFFAOYSA-L 0.000 claims 1
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 abstract description 10
- 229910001431 copper ion Inorganic materials 0.000 abstract description 10
- 238000010668 complexation reaction Methods 0.000 abstract description 2
- 230000002860 competitive effect Effects 0.000 abstract 1
- 230000000536 complexating effect Effects 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 241001530056 Athelia rolfsii Species 0.000 description 1
- 208000031404 Chromosome Aberrations Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 206010017533 Fungal infection Diseases 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 206010039509 Scab Diseases 0.000 description 1
- 235000003953 Solanum lycopersicum var cerasiforme Nutrition 0.000 description 1
- 240000003040 Solanum lycopersicum var. cerasiforme Species 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 231100000005 chromosome aberration Toxicity 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 208000024386 fungal infectious disease Diseases 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 208000019423 liver disease Diseases 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The invention discloses a rapid detection kit for pesticide carbendazim in fruit and vegetable products, which consists of a reaction tube and a carbendazim series standard solution; wherein copper acetate is arranged at the bottom of the reaction tube, and amaranth is fixed at the top of the reaction tube. The carbendazim series standard solutions were 0, 50, 100, 200, 500 and 1000ng/mL. The rapid detection method of the carbendazim is established through the competitive complexation between amaranth, the carbendazim and copper ions. The kit can realize the instant and rapid detection of carbendazim, and the detection limit is 200ng/mL. The carbendazim rapid detection kit has the advantages of low cost, short detection time and simple operation.
Description
Technical Field
The invention relates to a rapid detection kit and a detection method for pesticide carbendazim in fruit and vegetable products, belonging to the technical field of analytical chemistry.
Background
Carbendazim (carbendazim, bcm) is a low-toxicity, high-efficiency bactericide. It has good effects on rice blast, banded sclerotial blight, wheat scab, rape mycosis and the like, so that it is widely used in the production process of crops. Carbendazim can permeate into plants, is resistant to rain wash, has long residual effect period, and can cause hepatopathy and chromosome aberration due to residue, and is toxic to mammals. Therefore, it is important to detect carbendazim residues from the viewpoint of food safety or environmental protection.
Currently, the detection methods of carbendazim mainly comprise ultraviolet spectrophotometry, high performance liquid chromatography, immunoassay methods and the like. However, most of the methods are complicated in operation, and the instruments are expensive, so that the method is unfavorable for on-site visual rapid detection and analysis. The amino-hydroxyl group of carbendazim has high-efficiency complexing capability on copper ions, so that the colorimetric chromogenic technology based on metal complexing effect can be used for the instant visual rapid detection of carbendazim.
Disclosure of Invention
In view of the above, the invention provides a rapid detection kit and a detection method for pesticide carbendazim in fruit and vegetable products, and in order to achieve the above purposes, the technical scheme of the invention is as follows:
A rapid detection kit for pesticide carbendazim in fruit and vegetable products comprises a box body, wherein a reaction tube, a zinc acetate solution and a carbendazim series standard solution are arranged in the box body; the method is characterized in that copper acetate is arranged at the bottom of the reaction tube, and amaranth is fixed in the reaction tube cover. The reagent consists of 0, 50, 100, 200, 500 and 1000ng/mL carbendazim series standard solutions.
Preferably, the copper acetate content is 3-15 μg, and the amaranth content is 14-70 μg.
Preferably, the pretreatment steps of the fruit and vegetable samples are as follows:
10-20g of fruit and vegetable sample is placed in 5-25mL of methanol containing 25-125 mu L of zinc acetate, shake extraction is carried out for 1-5min, and then the solution is taken for testing.
The reaction cup is a polyethylene pipe with a cover and a volume of 1-5 mL; the series of standard solutions of the object to be detected are respectively obtained by dilution from pure objects to be detected, and the concentrations of the objects to be detected in the standard objects to be detected are respectively as follows: 50ng/mL, 100ng/mL, 200ng/mL, 500ng/mL, and 1000ng/mL.
The method for detecting the object to be detected by using the kit is based on copper ion complexation reaction. Adding 0.5-3mL carbendazim standard solution or sample extracting solution into the reaction tube, then covering the reaction tube cover, and vibrating the reaction tube to dissolve amaranth in the cover. Visual colorimetric detection of carbendazim is realized according to the color change of the solution.
Preferably, the detection limit of the present invention is defined as the concentration of carbendazim in a solution when the color of the solution is significantly different from the color of the solution without carbendazim.
Further, the detection limit of the kit on carbendazim is 200ng/mL.
Compared with the existing domestic and foreign carbendazim detection technology, the invention has the following outstanding advantages:
1. The copper ion complexing reaction realizes the instant visual rapid detection of carbendazim;
2. The number of the reaction tubes in the kit can be selected according to the detection requirement, the rapid detection and analysis of 100-500 samples can be completed within 1h, the kit is suitable for rapid screening of a large number of samples, and the kit can be used as an effective screening means for rapid detection of carbendazim in fruit and vegetable products.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention and do not constitute an undue limitation on the invention. In the drawings:
FIG. 1 is a graph showing the results of the detection of a standard solution of carbendazim according to the present invention.
FIG. 2 is a graph showing the result of detecting carbendazim in cherry tomatoes according to the invention.
Detailed Description
The invention will now be described in more detail with reference to the drawings in conjunction with the embodiments, but the examples given are not intended to limit the invention.
Example l (preparation example)
Preparation of carbendazim rapid detection kit
Fixation of copper acetate and amaranth: preparing copper acetate and amaranth solution with water, dripping 3-15 mu L of copper acetate at the bottom of a reaction tube, dripping 14-70 mu L of amaranth in a reaction tube cover, placing the reaction tube in a baking oven at 40 ℃ for drying for 20min, and then preserving in vacuum.
Example 2 (application example)
Application of carbendazim rapid detection kit
1. Detection step
Adding 0.5-3mL carbendazim standard solution or sample extracting solution into the reaction tube, then covering the reaction tube cover, and vibrating the reaction tube to dissolve amaranth in the cover. Visual colorimetric detection of carbendazim is realized according to the color change of the solution.
2. Result determination
The detection principle of the carbendazim rapid detection kit provided by the invention is as follows: the detection principle is as follows: the amaranth and copper ions are complexed to change red into yellow, the amino-hydroxyl group of the carbendazim is stronger than the azo-hydroxyl group of the amaranth than the copper ions, and when the carbendazim exists in the solution, the carbendazim competes for combining with the copper ions, so that the complexing amount of the amaranth is reduced, and the color of the solution changes from yellow into pink along with the increase of the concentration of the carbendazim. The detection limit of the present invention is defined as the concentration of carbendazim in a solution when the color of the solution is significantly different from the color of the solution without carbendazim. As shown in FIG. 2, the detection limit of the method of the present invention is 200ng/mL.
Example 3 (application example)
Application technology of carbendazim rapid detection kit
1. Pretreatment of fruit and vegetable products
10-20G of fruits and vegetables are placed in 5-25mL of methanol containing 25-125 mu L of zinc acetate, and the extracted solution is taken for detection after shaking extraction for 1-5 min. Dilution ratio= (methanol mass/fruit and vegetable mass)
2. Detection step
Adding 0.5-3mL of the pretreated methanol solution into a reaction tube, then covering the reaction tube, and vibrating the reaction tube to dissolve amaranth in the cover. Visual colorimetric detection of carbendazim is realized according to the color change of the solution.
3. Result determination
The detection principle of the carbendazim rapid detection kit provided by the invention is as follows: the amaranth and copper ions are complexed to change red into yellow, and the amino-hydroxyl group of the carbendazim has stronger copper ion complexing ability compared with the azo-hydroxyl group of the amaranth, when the carbendazim exists in the solution, the carbendazim competes for combining with the copper ions, so that the complexing amount of the amaranth is reduced, and the color of the solution is changed from yellow into pink along with the increase of the concentration of the carbendazim. The detection limit of a sample according to the invention is defined as the concentration of carbendazim in a solution when the color of the solution is significantly different from the color of the solution without carbendazim. As shown in FIG. 2, the detection limit of the method is 200ng/mL, and when the solution contains 200ng/mL carbendazim, the content of carbendazim in the fruit and vegetable sample is 200× (methanol mass/fruit and vegetable mass) ng/mL.
Claims (4)
1. A rapid detection kit for pesticide carbendazim in fruit and vegetable products comprises a box body, wherein a reaction tube, zinc acetate solution and a reagent are arranged in the box body; the preparation method is characterized in that copper acetate is arranged at the bottom of a reaction tube, amaranth is fixed in a reaction tube cover, and the reagent consists of 0, 50, 100, 200, 500 and 1000ng/mL carbendazim series standard solutions.
2. The rapid detection kit for pesticide carbendazim in fruit and vegetable products according to claim 1, wherein the reaction tube is made of polyethylene tube, and is provided with a cover, the volume is 1-5mL, the copper acetate content is 3-15 mug, and the amaranth content is 14-70 mug.
3. The method for detecting the pesticide carbendazim by using the kit of claim 1 is characterized in that 0.5-3.0mL of carbendazim standard solution or sample extracting solution is added into a reaction tube, then a reaction tube cover is covered, the reaction tube is vibrated to dissolve amaranth in the cover, and the visual colorimetric detection of the carbendazim is realized according to the color change of the solution.
4. The method for detecting pesticide carbendazim by using the kit according to claim 3, wherein the sample extracting solution is obtained by the following steps: 10-20g of fruit and vegetable sample is placed in 5-25mL of methanol containing 25-125 mu L of zinc acetate, and shake extraction is carried out for 1-5min, and the obtained zinc acetate methanol solution is the sample extracting solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111457195.9A CN114136961B (en) | 2021-12-01 | 2021-12-01 | Rapid detection kit and detection method for pesticide carbendazim in fruit and vegetable products |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111457195.9A CN114136961B (en) | 2021-12-01 | 2021-12-01 | Rapid detection kit and detection method for pesticide carbendazim in fruit and vegetable products |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114136961A CN114136961A (en) | 2022-03-04 |
| CN114136961B true CN114136961B (en) | 2024-05-14 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202111457195.9A Active CN114136961B (en) | 2021-12-01 | 2021-12-01 | Rapid detection kit and detection method for pesticide carbendazim in fruit and vegetable products |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101228441A (en) * | 2005-05-02 | 2008-07-23 | 勒瑞音菲炫股份有限公司 | Spectroscopic method for the detection of analytes |
| CN102313804A (en) * | 2011-02-01 | 2012-01-11 | 天津百鸥瑞达生物科技有限公司 | Method and enzyme-linked immunosorbent assay (ELISA) kit for detecting amaranth |
| WO2018213596A1 (en) * | 2017-05-17 | 2018-11-22 | Spogen Biotech Inc. | Devices, systems, and methods for agrochemical detection and agrochemical compositions |
| CN113721024A (en) * | 2021-09-16 | 2021-11-30 | 天津温阳生物技术有限公司 | Fluorescence immunoassay rapid detection kit and detection method for enrofloxacin carbon quantum dots in animal derived food |
-
2021
- 2021-12-01 CN CN202111457195.9A patent/CN114136961B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101228441A (en) * | 2005-05-02 | 2008-07-23 | 勒瑞音菲炫股份有限公司 | Spectroscopic method for the detection of analytes |
| CN102313804A (en) * | 2011-02-01 | 2012-01-11 | 天津百鸥瑞达生物科技有限公司 | Method and enzyme-linked immunosorbent assay (ELISA) kit for detecting amaranth |
| WO2018213596A1 (en) * | 2017-05-17 | 2018-11-22 | Spogen Biotech Inc. | Devices, systems, and methods for agrochemical detection and agrochemical compositions |
| CN113721024A (en) * | 2021-09-16 | 2021-11-30 | 天津温阳生物技术有限公司 | Fluorescence immunoassay rapid detection kit and detection method for enrofloxacin carbon quantum dots in animal derived food |
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| Publication number | Publication date |
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| CN114136961A (en) | 2022-03-04 |
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