CN114136961A - Rapid detection kit and detection method for pesticide carbendazim in fruit and vegetable products - Google Patents
Rapid detection kit and detection method for pesticide carbendazim in fruit and vegetable products Download PDFInfo
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- CN114136961A CN114136961A CN202111457195.9A CN202111457195A CN114136961A CN 114136961 A CN114136961 A CN 114136961A CN 202111457195 A CN202111457195 A CN 202111457195A CN 114136961 A CN114136961 A CN 114136961A
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- carbendazim
- reaction tube
- solution
- fruit
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- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 title claims abstract description 58
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 title claims abstract description 52
- 239000006013 carbendazim Substances 0.000 title claims abstract description 52
- 238000001514 detection method Methods 0.000 title claims abstract description 47
- 235000012055 fruits and vegetables Nutrition 0.000 title claims abstract description 17
- 239000000575 pesticide Substances 0.000 title claims abstract description 8
- 238000006243 chemical reaction Methods 0.000 claims abstract description 29
- 240000001592 Amaranthus caudatus Species 0.000 claims abstract description 19
- 235000009328 Amaranthus caudatus Nutrition 0.000 claims abstract description 19
- 235000012735 amaranth Nutrition 0.000 claims abstract description 19
- 239000004178 amaranth Substances 0.000 claims abstract description 19
- 239000012086 standard solution Substances 0.000 claims abstract description 10
- OPQARKPSCNTWTJ-UHFFFAOYSA-L copper(ii) acetate Chemical compound [Cu+2].CC([O-])=O.CC([O-])=O OPQARKPSCNTWTJ-UHFFFAOYSA-L 0.000 claims abstract description 8
- 239000000243 solution Substances 0.000 claims description 28
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 21
- 238000000034 method Methods 0.000 claims description 8
- ZOIORXHNWRGPMV-UHFFFAOYSA-N acetic acid;zinc Chemical compound [Zn].CC(O)=O.CC(O)=O ZOIORXHNWRGPMV-UHFFFAOYSA-N 0.000 claims description 6
- 239000003153 chemical reaction reagent Substances 0.000 claims description 6
- 230000000007 visual effect Effects 0.000 claims description 6
- 239000004246 zinc acetate Substances 0.000 claims description 6
- -1 polyethylene Polymers 0.000 claims description 5
- 239000004698 Polyethylene Substances 0.000 claims description 2
- 229920000573 polyethylene Polymers 0.000 claims description 2
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 abstract description 10
- 229910001431 copper ion Inorganic materials 0.000 abstract description 10
- 230000002860 competitive effect Effects 0.000 abstract 1
- 238000010668 complexation reaction Methods 0.000 abstract 1
- 230000000536 complexating effect Effects 0.000 description 9
- 238000005516 engineering process Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 208000031404 Chromosome Aberrations Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 206010017533 Fungal infection Diseases 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 235000003953 Solanum lycopersicum var cerasiforme Nutrition 0.000 description 1
- 240000003040 Solanum lycopersicum var. cerasiforme Species 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 231100000005 chromosome aberration Toxicity 0.000 description 1
- 238000012272 crop production Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 208000024386 fungal infectious disease Diseases 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 208000019423 liver disease Diseases 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The invention discloses a rapid detection kit for pesticide carbendazim in fruit and vegetable products, which consists of a reaction tube and carbendazim series standard solution; wherein the bottom of the reaction tube is provided with copper acetate, and the top of the reaction tube is fixed with amaranth. The carbendazim series standard solutions were 0, 50, 100, 200, 500 and 1000 ng/mL. Through competitive complexation among amaranth, carbendazim and copper ions, a rapid detection method of carbendazim is established. The kit can realize the instant and rapid detection of the carbendazim, and the detection limit is 200 ng/mL. The rapid detection kit for carbendazim has the advantages of low cost, short detection time and simple and convenient operation.
Description
Technical Field
The invention relates to a rapid detection kit for carbendazim in fruit and vegetable products and a detection method thereof, belonging to the technical field of analytical chemistry.
Background
Carbendazim (bcm) is a highly effective bactericide with low toxicity. It has good effect on rice blast, sheath blight, wheat head blight, rape mycosis, etc. so it is widely used in crop production. Carbendazim can penetrate into plants, is resistant to rain wash, has long residual effect period, and can cause liver diseases and chromosome aberration and be toxic to mammals. Therefore, the detection of the carbendazim residue is very important from the viewpoint of food safety and environmental protection.
At present, the detection methods of carbendazim mainly comprise an ultraviolet spectrophotometry method, a high performance liquid chromatography method, an immunoassay method and the like. However, most of these methods are complicated to operate, expensive in instruments, and not conducive to on-site visualization and rapid detection and analysis. The amino-hydroxyl group of the carbendazim has high-efficiency complexing ability to copper ions, so that the colorimetric color development technology based on metal complexing action can be used for the immediate visual rapid detection of the carbendazim.
Disclosure of Invention
In view of the above, the invention provides a rapid detection kit for carbendazim in fruit and vegetable products and a detection method thereof, and in order to achieve the above purpose, the technical scheme of the invention is realized as follows:
a rapid detection kit for pesticide carbendazim in fruit and vegetable products comprises a box body, wherein a reaction tube, a zinc acetate solution and a carbendazim series standard solution are arranged in the box body; it is characterized in that the bottom of the reaction tube is provided with copper acetate, and amaranth is fixed in the reaction tube cover. The reagents consist of 0, 50, 100, 200, 500 and 1000ng/mL carbendazim series standard solutions.
Preferably, the copper acetate content is 3-15 μ g, and the amaranth content is 14-70 μ g.
Preferably, the pretreatment steps of the fruit and vegetable sample are as follows:
placing 10-20g of fruit and vegetable samples in 5-25mL of methanol containing 25-125 μ L of zinc acetate, extracting for 1-5min by shaking, and taking the solution to be tested.
The reaction cup is a polyethylene pipe with a cover and the volume of which is 1-5 mL; the series of standard solutions of the object to be detected are obtained by diluting pure objects to be detected respectively, and the concentrations of the objects to be detected in the standard objects to be detected are respectively as follows: 50ng/mL, 100ng/mL, 200ng/mL, 500ng/mL, and 1000 ng/mL.
The method for detecting the substance to be detected by using the kit is based on the copper ion complexing reaction. Adding 0.5-3mL of carbendazim standard solution or sample extract into the reaction tube, then covering the reaction tube, and shaking the reaction tube to dissolve amaranth in the cover. And realizing the visual colorimetric detection of the carbendazim according to the color change of the solution.
Preferably, the detection limit of the present invention is defined as the concentration of carbendazim in a solution when the color of the solution is clearly distinguished from the color of the solution in the absence of carbendazim.
Further, the detection limit of the kit on the carbendazim is 200 ng/mL.
Compared with the existing carbendazim detection technology at home and abroad, the invention has the following outstanding advantages:
1. the copper ion complex reaction realizes the real-time visual rapid detection of carbendazim;
2. the quantity of the reaction tubes in the reagent kit can be selected according to the detection requirement, the rapid detection and analysis of 100 and 500 samples can be completed within 1h, the reagent kit is suitable for rapid screening of a large number of samples, and the reagent kit can be used as an effective screening means for rapid detection of multiple bacteria in fruit and vegetable products.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and, together with the description, serve to explain the invention without limitation. In the drawings:
FIG. 1 is a diagram showing the results of the detection of carbendazim standard solution according to the present invention.
FIG. 2 is a graph showing the results of detecting carbendazim in small cherry tomatoes according to the present invention.
Detailed Description
The invention will be described in more detail hereinafter with reference to the accompanying drawings, in which embodiments of the invention are shown, but the examples are not intended to limit the invention.
Example l (preparation example)
Preparation of carbendazim rapid detection kit
Fixation of copper acetate and amaranth: preparing copper acetate and amaranth solution with water, dripping 3-15 μ L of copper acetate at the bottom of the reaction tube, dripping 14-70 μ L of amaranth in the cover of the reaction tube, drying the reaction tube in an oven at 40 deg.C for 20min, and vacuum-preserving.
Example 2 (application example)
Application of carbendazim rapid detection kit
1. Detection step
Adding 0.5-3mL of carbendazim standard solution or sample extract into the reaction tube, then covering the reaction tube, and shaking the reaction tube to dissolve amaranth in the cover. And realizing the visual colorimetric detection of the carbendazim according to the color change of the solution.
2. Determination of results
The detection principle of the rapid detection kit for carbendazim is as follows: the detection principle is as follows: the red of amaranth becomes yellow after complexing with copper ions, the amino-hydroxyl group of carbendazim has stronger complexing ability than the azo-hydroxyl group of amaranth than the copper ions, and when the carbendazim exists in the solution, the carbendazim competes for combining the copper ions, so that the complexing amount of the amaranth is reduced, and the color of the solution is changed from yellow to pink along with the increase of the concentration of the carbendazim. The detection limit of the present invention is defined as the concentration of carbendazim in a solution when the color of the solution is clearly distinguished from the color of the solution in the absence of carbendazim. As shown in FIG. 2, the detection limit of the method of the present invention is 200 ng/mL.
Example 3 (application example)
Application technology of rapid detection kit for carbendazim
1. Pretreatment of fruit and vegetable products
Placing 10-20g of fruits and vegetables in 5-25mL of methanol containing 25-125 μ L of zinc acetate, extracting under shaking for 1-5min, and collecting the extractive solution for testing. Dilution multiple ═ methanol mass/fruit and vegetable mass)
2. Detection step
Adding 0.5-3mL of the pretreated methanol solution into the reaction tube, then covering the reaction tube, and shaking the reaction tube to dissolve amaranth in the cover. And realizing the visual colorimetric detection of the carbendazim according to the color change of the solution.
3. Determination of results
The detection principle of the rapid detection kit for carbendazim is as follows: the red of amaranth becomes yellow after complexing with copper ions, the amino-hydroxyl group of carbendazim has stronger copper ion complexing ability than the azo-hydroxyl group of amaranth, and when the carbendazim exists in the solution, the carbendazim competes for combining the copper ions, so that the complexing amount of the amaranth is reduced, and the solution color is changed from yellow to pink along with the increase of the concentration of the carbendazim. The limit of detection of a sample according to the present invention is defined as the concentration of carbendazim in a solution when the color of the solution is clearly distinguished from the color of the solution in the absence of carbendazim. As shown in figure 2, the detection limit of the method is 200ng/mL, and when the solution contains 200ng/mL of carbendazim, the content of carbendazim in the fruit and vegetable sample is 200 x (methanol quality/fruit and vegetable quality) ng/mL.
Claims (4)
1. A rapid detection kit for pesticide carbendazim in fruit and vegetable products comprises a box body, wherein a reaction tube, a zinc acetate solution and a reagent are arranged in the box body; the method is characterized in that copper acetate is arranged at the bottom of the reaction tube, amaranth is fixed in the reaction tube cover, and the reagent consists of 0, 50, 100, 200, 500 and 1000ng/mL carbendazim series standard solutions.
2. The rapid detection kit for the pesticide carbendazim in the fruit and vegetable products as claimed in claim 1, wherein the reaction tube is made of a polyethylene tube, is provided with a cover, and has a volume of 1-5mL, a copper acetate content of 3-15 μ g and an amaranth content of 14-70 μ g.
3. The method for detecting the pesticide carbendazim by using the kit as claimed in claim 1, which is characterized in that 0.5-3.0mL of carbendazim standard solution or sample extracting solution is added into a reaction tube, then a reaction tube cover is covered, the reaction tube is vibrated to dissolve amaranth in the cover, and the visual colorimetric detection of the carbendazim is realized according to the color change of the solution.
4. The method for detecting the pesticide carbendazim by using the kit as claimed in claim 3, wherein the sample extracting solution is obtained by the following steps: placing 10-20g of fruit and vegetable samples in 5-25mL of methanol containing 25-125 μ L of zinc acetate, and extracting for 1-5min by shaking to obtain a methanol solution of zinc acetate, namely a sample extracting solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111457195.9A CN114136961B (en) | 2021-12-01 | 2021-12-01 | Rapid detection kit and detection method for pesticide carbendazim in fruit and vegetable products |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111457195.9A CN114136961B (en) | 2021-12-01 | 2021-12-01 | Rapid detection kit and detection method for pesticide carbendazim in fruit and vegetable products |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114136961A true CN114136961A (en) | 2022-03-04 |
| CN114136961B CN114136961B (en) | 2024-05-14 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202111457195.9A Active CN114136961B (en) | 2021-12-01 | 2021-12-01 | Rapid detection kit and detection method for pesticide carbendazim in fruit and vegetable products |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101228441A (en) * | 2005-05-02 | 2008-07-23 | 勒瑞音菲炫股份有限公司 | Spectroscopic method for the detection of analytes |
| CN102313804A (en) * | 2011-02-01 | 2012-01-11 | 天津百鸥瑞达生物科技有限公司 | Method and enzyme-linked immunosorbent assay (ELISA) kit for detecting amaranth |
| WO2018213596A1 (en) * | 2017-05-17 | 2018-11-22 | Spogen Biotech Inc. | Devices, systems, and methods for agrochemical detection and agrochemical compositions |
| CN113721024A (en) * | 2021-09-16 | 2021-11-30 | 天津温阳生物技术有限公司 | Fluorescence immunoassay rapid detection kit and detection method for enrofloxacin carbon quantum dots in animal derived food |
-
2021
- 2021-12-01 CN CN202111457195.9A patent/CN114136961B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101228441A (en) * | 2005-05-02 | 2008-07-23 | 勒瑞音菲炫股份有限公司 | Spectroscopic method for the detection of analytes |
| CN102313804A (en) * | 2011-02-01 | 2012-01-11 | 天津百鸥瑞达生物科技有限公司 | Method and enzyme-linked immunosorbent assay (ELISA) kit for detecting amaranth |
| WO2018213596A1 (en) * | 2017-05-17 | 2018-11-22 | Spogen Biotech Inc. | Devices, systems, and methods for agrochemical detection and agrochemical compositions |
| CN113721024A (en) * | 2021-09-16 | 2021-11-30 | 天津温阳生物技术有限公司 | Fluorescence immunoassay rapid detection kit and detection method for enrofloxacin carbon quantum dots in animal derived food |
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| CN114136961B (en) | 2024-05-14 |
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