CN114113653A

CN114113653A - Anti-pollution nucleic acid detecting tube for preventing liquid leakage and application thereof

Info

Publication number: CN114113653A
Application number: CN202111507441.7A
Authority: CN
Inventors: 刘娟; 杨毅跃; 宗小平; 齐树合
Original assignee: Yancheng Kailong Pharmaceutical Co ltd
Current assignee: Yancheng Kailong Pharmaceutical Co ltd
Priority date: 2021-12-10
Filing date: 2021-12-10
Publication date: 2022-03-01
Anticipated expiration: 2041-12-10
Also published as: CN114113653B

Abstract

The invention relates to the technical field of nucleic acid detecting tubes, and discloses a liquid leakage prevention anti-pollution nucleic acid detecting tube which comprises a tube body, wherein a base is arranged at the bottom of the tube body, a sucking disc is arranged at the bottom of the base, a plug head is arranged at the top of the tube body, a plug rod is fixedly connected to the bottom end of the plug head, a rubber block is fixedly connected to the bottom end of the plug rod, a liquid seepage pore passage penetrates through the interior of the plug head, filter membrane paper is arranged at the top end of the plug head, an accommodating cavity is arranged in the tube body, a cavity opening is formed in the top of the accommodating cavity, a sealing ring is arranged at the top of the cavity opening, a threaded hole is formed in the tube body shell, a fastening bolt is arranged in the threaded hole, a threaded tube is arranged in the tube body shell, and an internal thread is formed in the center of the threaded tube. The anti-pollution nucleic acid detecting tube of prevention of liquid leakage has the anti-pollution effect of prevention of liquid leakage, can solve current nucleic acid detecting tube general structure all comparatively simple, and operating personnel handles and the transfer process in, takes place to leak easily, causes the pollution problem for the sample that detects.

Description

Anti-pollution nucleic acid detecting tube for preventing liquid leakage and application thereof

Technical Field

The invention relates to the field of medical instruments, in particular to a liquid leakage prevention and pollution prevention nucleic acid detection tube and application thereof.

Background

Rapid nucleic acid detection is an important component of many applications in human health and biotechnology, including the detection of infectious agents, agricultural pathogens, or circulating DNA or RNA associated with disease.

The existing nucleic acid detection tube for detecting nucleic acid is simple in structure, leakage is easy to occur in the processes of handling and transferring by operators, pollution is caused to detection sampling, and the risk of infection of the operators is increased; and the existing nucleic acid detecting tube for detecting nucleic acid has poor sealing property, is not portable and limits the application in the field.

Disclosure of Invention

Aiming at the defects of the prior art, the invention provides a liquid leakage prevention and pollution prevention nucleic acid detection tube and application thereof, which can solve the problems that the general structure of the existing nucleic acid detection tube for nucleic acid detection is simpler, leakage is easy to occur in the processing and transferring processes of operators, the sampling of detection is polluted, and the risk of the operators being infected is increased; the invention effectively solves the problems of the prior art by arranging the anti-leakage and anti-pollution nucleic acid detecting tube and the application thereof.

In order to realize the above-mentioned prevention of liquid leakage and pollution nucleic acid detecting tube and its application purpose, the invention provides the following technical scheme: a liquid leakage prevention anti-pollution nucleic acid detecting tube and application thereof comprise a tube body, wherein a base is arranged at the bottom of the tube body, a sucker is arranged at the bottom of the base, a cock head is arranged at the top of the tube body, a cock rod is fixedly connected to the bottom end of the cock head, a rubber block is fixedly connected to the bottom end of the cock rod, a liquid seepage pore passage is arranged in the cock head in a penetrating manner, filter membrane paper is arranged at the top end of the cock head, a containing cavity is arranged in the tube body, a cavity opening is formed in the top of the containing cavity, a sealing ring is arranged at the top of the cavity opening, a threaded hole is formed in a tube body shell, a fastening bolt is arranged in the threaded hole, a threaded tube is arranged in the tube body shell, an internal thread is formed in the center of the threaded tube, a fixing plate is arranged on the inner wall of the tube body shell, a spring is arranged between the threaded tube and the fixing plate, and a visual window is arranged on the front face of the tube body, the front surface of the cylinder body is provided with a bar code.

Preferably, the sealing ring is cylindrical, the sealing ring is fixedly connected inside the shell of the barrel body, the inner diameter of the sealing ring is matched with the outer diameter of the plug rod, and the plug rod is movably inserted inside the sealing ring and is in threaded connection with the sealing ring.

Preferably, the threaded holes are symmetrically distributed on two sides of the sealing ring, the threaded holes penetrate through the inner wall and the outer wall of the cylinder shell and the inner wall and the outer wall of the sealing ring shell and extend to the inner cavity of the sealing ring, the outer diameter of the fastening bolt is matched with the inner diameter of the threaded hole, and the fastening bolt is movably inserted into the threaded holes and is in threaded connection with the threaded holes.

Preferably, the inner diameter of the threaded cylinder is matched with the outer diameter of the plug rod, and the plug rod penetrates through the threaded cylinder and is inserted into the threaded cylinder and in threaded connection with the threaded cylinder.

Preferably, the fixing plates are symmetrically distributed on two sides of the cavity opening and fixedly connected with the inner wall of the barrel body, the springs are symmetrically distributed on two sides of the cavity opening, one end of each spring is fixedly connected with the threaded barrel, and the other end of each spring is fixedly connected with the fixing plate.

Preferably, the bore diameter size of the cavity opening is matched with the outer diameter size of the rubber block, the outer diameter size of the rubber block is slightly larger than the bore diameter size of the cavity opening, the rubber block is movably clamped inside the cavity opening, the liquid seepage pore is located on the central axis of the plug head, the liquid seepage pore runs through the plug head, and the bottom of the liquid seepage pore is communicated with the cavity inner cavity.

Preferably, the visual window is made of transparent plastic, the barrel body and the base are made of plastic, and the sucking disc, the sealing ring and the rubber block are made of rubber.

Preferably, the bar codes record user identity information, and one bar code corresponds to one user identity information.

Compared with the prior art, the invention provides a liquid leakage prevention and pollution prevention nucleic acid detection tube and application thereof, and the detection tube has the following beneficial effects:

1. according to the invention, the sealing ring is arranged at the top of the barrel, the bottom end of the plug head is connected with the plug rod, the bottom end of the plug rod is connected with the rubber block, the plug head is used for sealing the barrel, the rubber block arranged at the bottom end of the plug head is clamped in the cavity opening, the sealing property of the cavity opening port can be ensured, the sealing ring at the periphery of the plug rod can ensure the sealing property of the connection part of the plug rod and the inner gap of the barrel shell, and the slurry leakage phenomenon of liquid in the accommodating cavity can be effectively avoided.

2. According to the invention, the fastening bolts are arranged on the two sides of the sealing ring, the fastening bolts can be used for locking the plug rod from one side of the plug rod by screwing the fastening bolts, the fastening effect is good, the stability of the plug rod after installation is ensured, meanwhile, the threaded barrel is in threaded connection with the periphery of the plug rod, the spring is arranged at the bottom of the threaded barrel, the vibration can be buffered by utilizing the elastic expansion and contraction of the spring after the plug rod is installed, the stability of the plug rod after installation is improved, and the phenomenon that liquid in the accommodating cavity leaks from a gap of the plug rod due to the looseness of the plug rod caused by external collision or stress is avoided.

3. According to the invention, the sucker is arranged at the bottom of the barrel body, so that the barrel body is conveniently sucked at a specific position and is convenient to place, the portability is improved, and the use is convenient.

4. The existing nucleic acid detecting tube is made of plastic material, is sealed by extrusion and is easy to leak, a tube cover is opened to drip a reagent during detection, the invention can detect without opening the tube cover, a penetrating weeping pore canal is arranged at the central axis of a cock head, a filter membrane paper is arranged at one end of the cock head, when detecting nucleic acid, a needle head is firstly used for puncturing by a specific machine, then the detecting reagent is dripped into the weeping pore canal, the detecting reagent permeates into a containing cavity through the weeping pore canal under the action of gravity, the cock head does not need to be taken down, the needle head is not polluted, a visual window is arranged on the front surface of a cylinder body, the color of the nucleic acid after reaction with the detecting reagent can be changed, the result can be directly displayed on the bottle body through the visual window, the computer vision is used for transmitting the visual window, the display color of the reagent in the bottle can be automatically identified through an artificial intelligence algorithm, so as to make the detection result, and based on the bar code arranged on the barrel body, the bar code is matched with the users (each user has the bar code), and the bar code is directly stored on the network, so that the whole process is free of manual operation, and infection is avoided.

Drawings

FIG. 1 is a schematic perspective view of the present invention;

FIG. 2 is a schematic front view of the present invention;

FIG. 3 is a schematic view of the internal structure of the barrel according to the present invention;

FIG. 4 is a schematic view of the threaded cylinder of the present invention;

FIG. 5 is an enlarged view of a portion of FIG. 3 according to the present invention;

FIG. 6 is a front cross-sectional structural view of the present invention;

fig. 7 is a schematic structural view of the plug head of the present invention.

In the figure: 1. a barrel; 2. a base; 3. a suction cup; 4. a plug head; 41. a cock stem; 42. a rubber block; 43. a weeping channel; 44. filtering membrane paper; 5. a cavity; 6. a lumen port; 7. a seal ring; 8. a threaded hole; 9. fastening a bolt; 10. a threaded barrel; 101. an internal thread; 11. a fixing plate; 12. a spring; 13. a visual window; 14. a bar code.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-7, a liquid leakage prevention and pollution prevention nucleic acid detecting tube and the application thereof, comprising a tube body 1, wherein a base 2 is arranged at the bottom of the tube body 1, a sucking disc 3 is arranged at the bottom of the base 2, a cock head 4 is arranged at the top of the tube body 1, a cock rod 41 is fixedly connected to the bottom end of the cock head 4, a rubber block 42 is fixedly connected to the bottom end of the cock rod 41, a liquid seepage pore canal 43 is arranged in the cock head 4 in a penetrating manner, a filter membrane paper 44 is arranged at the top end of the cock head 4, a containing cavity 5 is arranged in the tube body 1, a cavity opening 6 is arranged at the top of the containing cavity 5, a sealing ring 7 is arranged at the top of the cavity opening 6, a threaded hole 8 is arranged in the shell of the tube body 1, a fastening bolt 9 is arranged in the threaded hole 8, a threaded tube 10 is arranged in the shell of the tube body 1, an internal thread 101 is arranged at the center of the threaded tube 10, a fixing plate 11 is arranged on the inner wall of the shell of the tube body 1, a spring 12 is arranged between the threaded tube 10 and the fixing plate 11, the front surface of the cylinder body 1 is provided with a visual window 13, and the front surface of the cylinder body 1 is provided with a bar code 14.

In summary, the sealing ring 7 is cylindrical, the sealing ring 7 is fixedly connected inside the shell of the cylinder body 1, the inner diameter of the sealing ring 7 is matched with the outer diameter of the plug rod 41, the plug rod 41 is movably inserted inside the sealing ring 7 and is in threaded connection with the sealing ring 7, and the plug rod 41 is movably inserted inside the sealing ring 7 and is in threaded connection with the sealing ring 7 for sealing the cylinder body 1; the threaded holes 8 are symmetrically distributed on two sides of the sealing ring 7, the threaded holes 8 penetrate through the inner wall and the outer wall of the shell of the barrel body 1 and the shell of the sealing ring 7 and extend to the inner cavity of the sealing ring 7, the outer diameter size of the fastening bolt 9 is matched with the inner diameter size of the threaded hole 8, the fastening bolt 9 is movably inserted into the threaded hole 8 and in threaded connection with the threaded hole 8, the fastening bolt 9 can be screwed up to lock the plug rod 41 from one side of the plug rod 41, the fastening effect is good, and the stability of the plug rod 41 after installation is guaranteed; the inner diameter of the threaded cylinder 10 is matched with the outer diameter of the plug rod 41, the plug rod 41 penetrates through the threaded cylinder 10 and is connected with the threaded cylinder 10 in a threaded mode, the fixing plates 11 are symmetrically distributed on two sides of the cavity opening 6 and are fixedly connected with the inner wall of the cylinder body 1, the springs 12 are symmetrically distributed on two sides of the cavity opening 6, one end of each spring 12 is fixedly connected with the threaded cylinder 10, the other end of each spring 12 is fixedly connected with the fixing plate 11, and the spring 12 is arranged at the bottom of the threaded cylinder 10 and can buffer vibration by means of elastic expansion and contraction of the spring 12 after the plug rod 41 is installed, so that the stability of the plug rod 41 after installation is improved; the inner diameter of the cavity opening 6 is matched with the outer diameter of the rubber block 42, the outer diameter of the rubber block 42 is slightly larger than the inner diameter of the cavity opening 6, the rubber block 42 is movably clamped inside the cavity opening 6, the liquid seepage pore 43 is positioned on the central axis of the plug head 4, the liquid seepage pore 43 is arranged inside the plug head 4 in a penetrating mode, the bottom of the liquid seepage pore 43 is communicated with the inner cavity of the accommodating cavity 5, the rubber block 42 can guarantee the sealing performance of the cavity opening 6, a detection reagent is dripped into the liquid seepage pore 43, and the detection reagent can seep into the accommodating cavity 5 through the liquid seepage pore 43 under the action of gravity; the visual window 13 is made of transparent plastic, the cylinder body 1 and the base 2 are made of plastic, the sucker 3, the sealing ring 7 and the rubber block 42 are made of rubber, and the materials are cheap materials which can be directly used for waste treatment; the bar codes 14 record user identity information, one bar code 14 corresponds to one user identity information, users (each user has the bar code 14) are matched based on the bar codes 14 arranged on the barrel body 1 and are directly stored on a network, manual operation is not needed in the whole process, and infection is avoided.

The working use process and the installation method of the invention are that when the anti-contamination nucleic acid detecting tube for preventing liquid leakage is used, the extracted nucleic acid solution to be detected is added through the inner cavity of the sealing ring 7, the inner cavity of the threaded cylinder 10 and the inner cavity of the cavity opening 6 are communicated, then the nucleic acid solution enters the cavity 5 through the inner cavity of the sealing ring 7, the inner cavity of the threaded cylinder 10 and the inner cavity of the cavity opening 6, after the nucleic acid solution enters the cavity 5, the barrel 1 is sealed by the plug 4, the plug rod 41 is screwed into the inner cavity of the sealing ring 7, the rubber block 42 is clamped into the opening of the cavity opening 6 to seal the cavity 5, the sealing ring 7 is arranged at the top of the barrel 1, the bottom end of the plug 4 is connected with the plug rod 41, the bottom end of the plug rod 41 is connected with the rubber block 42, the barrel 1 is sealed through the plug 4, the rubber block 42 arranged at the bottom end of the plug 4 is clamped into the cavity opening 6, the sealing performance of the port of the cavity opening 6 and the sealing ring 7 on the periphery of the plug rod 41 can be ensured, the sealing performance of the joint of the plug rod 41 and the inner gap of the shell of the barrel body 1 can be ensured, and the phenomenon of slurry leakage of liquid in the accommodating cavity 5 can be effectively avoided; after the plug rod 41 is screwed in the sealing ring 7, the fastening bolts 9 are arranged on two sides of the sealing ring 7, the fastening bolts 9 can be used for locking the plug rod 41 from one side of the plug rod 41 by screwing the fastening bolts 9, the fastening effect is good, the stability after the plug rod 41 is screwed and installed is ensured, after the plug rod 41 is screwed in the sealing ring 7, the thread cylinder 10 is connected to the periphery of the plug rod 41 in a threaded manner, the spring 12 is arranged at the bottom of the thread cylinder 10, the vibration can be buffered by utilizing the elastic expansion and contraction of the spring 12 after the plug rod 41 is installed, the stability after the plug rod 41 is installed is improved, and the phenomenon that the liquid in the accommodating cavity 5 leaks from the gap of the plug rod 41 due to the looseness caused by external collision or stress of the plug rod 41 is avoided; the bottom of the cylinder body 1 is provided with the sucking disc 3, so that the cylinder body 1 can be conveniently adsorbed at a specific position, the cylinder body is convenient to place, the portability is improved, the use is convenient, in addition, the existing nucleic acid detection tube is made of plastic materials, the nucleic acid detection tube is sealed by extrusion and is easy to leak, and a reagent is dripped into the nucleic acid detection tube by opening the tube cap, the detection can be realized without opening the tube cap, the detection reagent is dripped into the liquid seepage hole 43 by the puncture of a needle head of a specific machine through the penetrating liquid seepage hole 43 arranged at the central axis of the plug head 4 and the filter membrane paper 44 arranged at one end of the plug head 4 during the nucleic acid detection, the detection reagent is firstly punctured by the needle head of the specific machine and then is dripped into the liquid seepage hole 43, the detection reagent is infiltrated into the containing cavity 5 under the action of gravity, the plug head 4 does not need to be taken down, the needle head is not polluted, the color of the nucleic acid can be changed after the nucleic acid reacts with the detection reagent by arranging the visual window 13 on the front side of the cylinder body, the visual window 13 can directly display the result on the bottle body, the computer vision is utilized to penetrate through the visual window 13, the display color of the reagent in the bottle after reaction is automatically identified through an artificial intelligence algorithm so as to make a detection result, and the bar code 14 arranged on the barrel body 1 is matched with a user (each user has a unique bar code 14) and is directly stored on a network, so that the whole process is free of manual operation, and the infection is avoided. For example, a camera is arranged in front of the visible window 13, and the camera can shoot the color displayed after the reagent in the bottle reacts. Comparing the color in the picture shot by the camera with the standard color through a computer vision algorithm and an artificial intelligence algorithm (for example, the trained neural network), and judging whether the color reaches the standard color so as to judge the detection result. After the detection result is determined, the bar code 14 is identified through the camera and matched with a user personal account or an information base, the detection result is stored in the user personal account or the information base, the whole process is automatically completed, manual intervention is not needed, and the risk of infection caused by leakage in the transportation and detection processes is greatly reduced for viruses with extremely high infectivity, such as new coronavirus.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A liquid leakage prevention and pollution prevention nucleic acid detection tube is characterized in that: the novel multifunctional tap comprises a barrel body (1), wherein a base (2) is arranged at the bottom of the barrel body (1), a sucker (3) is arranged at the bottom of the base (2), a tap head (4) is arranged at the top of the barrel body (1), a tap rod (41) is fixedly connected to the bottom of the tap head (4), a rubber block (42) is fixedly connected to the bottom of the tap rod (41), a liquid seepage hole (43) penetrates through the inside of the tap head (4), filter membrane paper (44) is arranged at the top end of the tap head (4), a cavity (5) is arranged inside the barrel body (1), a cavity opening (6) is formed at the top of the cavity (5), a sealing ring (7) is arranged at the top of the cavity opening (6), a threaded hole (8) is formed in the shell of the barrel body (1), a fastening bolt (9) is arranged inside the threaded hole (8), and a threaded barrel (10) is arranged inside the shell of the barrel body (1), an internal thread (101) is formed in the center of the threaded cylinder (10), a fixing plate (11) is arranged on the inner wall of a shell of the cylinder body (1), a spring (12) is arranged between the threaded cylinder (10) and the fixing plate (11), a visual window (13) is arranged on the front face of the cylinder body (1), and a bar code (14) is arranged on the front face of the cylinder body (1).

2. The anti-leakage and anti-contamination nucleic acid detecting tube according to claim 1, wherein: the sealing ring (7) is cylindrical, the sealing ring (7) is fixedly connected inside the shell of the barrel body (1), the inner diameter of the sealing ring (7) is matched with the outer diameter of the plug rod (41), and the plug rod (41) is movably inserted inside the sealing ring (7) and is in threaded connection with the sealing ring (7).

3. The anti-leakage and anti-contamination nucleic acid detecting tube according to claim 1, wherein: the threaded holes (8) are symmetrically distributed on two sides of the sealing ring (7), the threaded holes (8) penetrate through the inner wall and the outer wall of the shell of the barrel body (1) and the shell of the sealing ring (7) and extend to the inner cavity of the sealing ring (7), the outer diameter of the fastening bolt (9) is matched with the inner diameter of the threaded hole (8), and the fastening bolt (9) is movably inserted into the threaded hole (8) and is in threaded connection with the threaded hole (8).

4. The anti-leakage and anti-contamination nucleic acid detecting tube according to claim 1, wherein: the inner diameter of the threaded cylinder (10) is matched with the outer diameter of the plug rod (41), and the plug rod (41) penetrates through the threaded cylinder (10) and is connected with the threaded cylinder (10) in a threaded mode.

5. The anti-leakage and anti-contamination nucleic acid detecting tube according to claim 1, wherein: fixed plate (11) symmetric distribution is in accent (6) both sides and with barrel (1) inner wall fixed connection, spring (12) symmetric distribution is in accent (6) both sides, spring (12) one end and screw thread section of thick bamboo (10) fixed connection, the other end and fixed plate (11) fixed connection.

6. The anti-leakage and anti-contamination nucleic acid detecting tube according to claim 1, wherein: cavity mouth (6) internal diameter size and rubber block (42) external diameter size looks adaptation, rubber block (42) external diameter size slightly is greater than cavity mouth (6) internal diameter size, rubber block (42) activity joint is inside in cavity mouth (6), sepage pore (43) are located cock head (4) axis department, sepage pore (43) run through the setting inside cock head (4), sepage pore (43) bottom link up with holding chamber (5) inner chamber.

7. The anti-leakage and anti-contamination nucleic acid detecting tube according to claim 1, wherein: the visual window (13) is made of transparent plastic, the barrel body (1) and the base (2) are made of plastic, and the sucker (3), the sealing ring (7) and the rubber block (42) are made of rubber.

8. The anti-leakage and anti-contamination nucleic acid detecting tube according to claim 1, wherein: the bar codes (14) record user identity information, and one bar code (14) corresponds to one user identity information.

9. The application of the anti-pollution nucleic acid detecting tube of the leakage-proof liquid is characterized in that: the use of a liquid-leakage-preventing and contamination-preventing nucleic acid detecting tube according to claims 1 to 8 for nucleic acid detection sampling.