CN114113490A - System and method for simulating and detecting lung gas exchange in diving decompression sickness process - Google Patents
System and method for simulating and detecting lung gas exchange in diving decompression sickness process Download PDFInfo
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- CN114113490A CN114113490A CN202111488279.9A CN202111488279A CN114113490A CN 114113490 A CN114113490 A CN 114113490A CN 202111488279 A CN202111488279 A CN 202111488279A CN 114113490 A CN114113490 A CN 114113490A
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- 210000004072 lung Anatomy 0.000 title claims abstract description 104
- 238000000034 method Methods 0.000 title claims abstract description 71
- 230000009189 diving Effects 0.000 title claims abstract description 28
- 206010011951 Decompression Sickness Diseases 0.000 title claims abstract description 23
- 239000007789 gas Substances 0.000 claims abstract description 153
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 64
- 239000001301 oxygen Substances 0.000 claims abstract description 64
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 64
- 239000000523 sample Substances 0.000 claims abstract description 40
- 239000012528 membrane Substances 0.000 claims abstract description 26
- 210000002919 epithelial cell Anatomy 0.000 claims abstract description 18
- 210000003556 vascular endothelial cell Anatomy 0.000 claims abstract description 13
- 238000001514 detection method Methods 0.000 claims abstract description 11
- 238000004090 dissolution Methods 0.000 claims abstract description 11
- 238000004088 simulation Methods 0.000 claims abstract description 9
- 229920006395 saturated elastomer Polymers 0.000 claims description 12
- 230000002685 pulmonary effect Effects 0.000 claims description 10
- 230000006837 decompression Effects 0.000 claims description 6
- 201000010099 disease Diseases 0.000 claims description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 6
- 238000009423 ventilation Methods 0.000 claims description 3
- 210000004204 blood vessel Anatomy 0.000 abstract description 3
- 210000002889 endothelial cell Anatomy 0.000 abstract description 3
- 230000004199 lung function Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
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- G—PHYSICS
- G09—EDUCATION; CRYPTOGRAPHY; DISPLAY; ADVERTISING; SEALS
- G09B—EDUCATIONAL OR DEMONSTRATION APPLIANCES; APPLIANCES FOR TEACHING, OR COMMUNICATING WITH, THE BLIND, DEAF OR MUTE; MODELS; PLANETARIA; GLOBES; MAPS; DIAGRAMS
- G09B23/00—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes
- G09B23/28—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes for medicine
Abstract
The invention relates to a lung gas exchange simulation detection system and a method in the process of diving decompression sickness, which comprises the following steps: the semi-permeable membrane carrier divides the box body into an upper chamber and a lower chamber, culture solution is filled in the first chamber and the second chamber, a first dissolved oxygen probe is arranged on the inner wall of the first chamber, a lung epithelial cell layer is arranged on the upper layer of the semi-permeable membrane carrier, a blood vessel endothelial cell layer is arranged on the lower layer of the semi-permeable membrane carrier, a second dissolved oxygen probe is arranged on the inner wall of the second chamber, and the top of the second chamber is communicated with a vent pipe; the preset gas introduced through the vent pipe is dispersed and sequentially enters the culture solution of the second chamber, the lung epithelial cell layer, the semipermeable membrane carrier, the vascular endothelial cell layer and the culture solution of the first chamber to simulate the lung gas saturation process, the first dissolved oxygen probe and the second dissolved oxygen probe respectively detect the first content value and the second content value in the culture solution of the first chamber and the second chamber, and the controller calculates the real-time lung gas exchange rate and the real-time lung gas dissolution rate in the culture solution of the first chamber.
Description
Technical Field
The invention relates to the technical field of medical instruments, in particular to a lung gas exchange simulation detection system and method in a diving decompression sickness process.
Background
The research on the mechanism of the diving decompression sickness lacks a related saturation/desaturation research device, the structure and the function of a breathing membrane play a key role in saturation/desaturation, and the research on a technology for simulating and detecting the lung gas exchange in the diving decompression sickness process is particularly important in order to solve the gas saturation/desaturation process in the diving decompression sickness process and evaluate the related technical indexes of the lung function.
Disclosure of Invention
Aiming at the problems and the defects in the prior art, the invention provides a novel lung gas exchange simulation detection system and method in the process of diving decompression sickness.
The invention solves the technical problems through the following technical scheme:
the invention provides a lung gas exchange simulation detection system in a diving decompression sickness process, which is characterized by comprising a sealed box body, wherein a semipermeable membrane carrier is fixed at the middle position in the sealed box body to divide the sealed box body into a first cavity and a second cavity up and down, a culture solution is loaded in the first cavity, a first dissolved oxygen probe is fixed on the inner wall of the first cavity, so that the upper layer of the semipermeable membrane carrier is provided with a plurality of lung epithelial cell layers, the lower layer of the semipermeable membrane carrier is provided with a blood vessel endothelial cell layer, the second cavity is loaded with the culture solution, a second dissolved oxygen probe is fixed on the inner wall of the second cavity, the top of the second cavity is provided with an air vent, the air vent is fixedly communicated with a vent pipe communicated with set gas, and the first dissolved oxygen probe and the second dissolved oxygen probe are electrically connected with a controller.
The method comprises the steps that set gas introduced through the ventilation pipe is dispersed and sequentially enters culture solution of a second chamber, a lung epithelial cell layer, a semipermeable membrane carrier, a vascular endothelial cell layer and culture solution of the first chamber to simulate a lung gas saturation process in a diving decompression disease process, the first dissolved oxygen probe is used for detecting the content of dissolved oxygen in the culture solution of the first chamber in real time to serve as a first content value, the second dissolved oxygen probe is used for detecting the content of dissolved oxygen in the culture solution of the second chamber in real time to serve as a second content value, the controller is used for recording the received first content value, corresponding time, the second content value and corresponding time in real time, the real-time exchange rate of the lung gas is calculated as the first content value of the current time/the second content value of the current time, and the real-time dissolution rate of the lung gas is calculated as (the current first content value in the culture solution of the first chamber-the first content value of the gas just entering in the culture solution of the first chamber)/the current time and the current time The time between the first entry of gas.
Stopping introducing the set gas after the gas is saturated, dispersing the gas in the culture solution of the first cavity in sequence to obtain the culture solution of the first cavity, a vascular endothelial cell layer, a semipermeable membrane carrier, a pulmonary epithelial cell layer and the culture solution of the second cavity so as to simulate the desaturation process of the lung gas in the process of the diving decompression disease, wherein the first dissolved oxygen probe is used for detecting the content of dissolved oxygen in the culture solution of the first cavity in real time as a third content value, the second dissolved oxygen probe is used for detecting the content of dissolved oxygen in the culture solution of the second cavity in real time as a fourth content value, the controller is used for recording the received third content value and corresponding time, the fourth content value and corresponding time in real time, calculating the real-time lung gas exchange rate which is the fourth content value of the current time/the third content value of the current time, and calculating the real-time lung gas desaturation rate which is the third content value of the current time when the gas in the culture solution of the first cavity is saturated Time between current time and gas saturation time.
Preferably, the system further comprises a display screen, the display screen is electrically connected to the controller, and the display screen is configured to display the real-time lung gas exchange rate and the real-time lung gas dissolution rate in the lung gas saturation process, and the real-time lung gas exchange rate and the real-time lung gas desaturation rate in the lung gas desaturation process.
The invention also provides a lung gas exchange simulation detection method in the process of the diving decompression sickness, which is characterized by being realized by utilizing the lung gas exchange simulation detection system, and the method comprises the following steps:
lung gas saturation procedure:
s11, introducing set gas into the vent pipe;
s12, setting gas to diffuse into the culture solution of the second chamber, the lung epithelial cell layer, the semi-permeable membrane carrier, the vascular endothelial cell layer and the culture solution of the first chamber in sequence so as to simulate the lung gas saturation process in the process of diving decompression sickness;
s13, detecting the content of dissolved oxygen in the culture solution of the first chamber as a first content value in real time by the first dissolved oxygen probe, and detecting the content of dissolved oxygen in the culture solution of the second chamber as a second content value in real time by the second dissolved oxygen probe;
s14, the controller records the received first content value and the corresponding time, the second content value and the corresponding time in real time, calculates the real-time exchange rate of the lung gas (the first content value in the culture solution in the first chamber-the first content value when the gas just enters in the culture solution in the first chamber)/the time between the current time and the time when the gas just enters in the first chamber);
lung gas desaturation process:
s21, stopping introducing the set gas after the gas is saturated;
s22, dispersing the gas in the culture solution of the first cavity in sequence to obtain the culture solution of the first cavity, the vascular endothelial cell layer, the semipermeable membrane carrier, the pulmonary epithelial cell layer and the culture solution of the second cavity so as to simulate the desaturation process of the lung gas in the process of diving decompression sickness;
s23, detecting the content of dissolved oxygen in the culture solution of the first chamber as a third content value in real time by the first dissolved oxygen probe, and detecting the content of dissolved oxygen in the culture solution of the second chamber as a fourth content value in real time by the second dissolved oxygen probe;
s24, the controller records the received third content value and the corresponding time, the received fourth content value and the corresponding time in real time, calculates the real-time lung gas exchange rate as the fourth content value of the current time/the third content value of the current time, and calculates the real-time lung gas desaturation rate as the third content value of the first chamber when the gas is saturated in the culture solution-the third content value of the current time/the time between the current time and the gas saturation time.
Preferably, step S14 is followed by the following steps: s15, displaying the real-time exchange rate and the real-time dissolution rate of the lung gas in the lung gas saturation process by a display screen;
step S24 is followed by the steps of: and S25, displaying the real-time lung gas exchange rate and the real-time lung gas desaturation rate in the lung gas desaturation process by the display screen.
On the basis of the common knowledge in the field, the above preferred conditions can be combined randomly to obtain the preferred embodiments of the invention.
The positive progress effects of the invention are as follows:
the invention can well simulate the gas saturation/desaturation process in the process of detecting the diving decompression sickness and calculate and evaluate the related technical indexes of the lung function.
Drawings
Fig. 1 is a schematic structural diagram of a lung gas exchange simulation detection system according to a preferred embodiment of the invention.
Fig. 2 is a flow chart of lung gas saturation according to a preferred embodiment of the present invention.
FIG. 3 is a flow chart of lung gas desaturation according to a preferred embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
As shown in fig. 1, the present embodiment provides a lung gas exchange simulation detection system in a diving decompression sickness process, which includes a sealed box body 1, a semipermeable membrane carrier 2 is fixed at a middle position in the sealed box body 1 to divide the sealed box body 1 into a first chamber 3 and a second chamber 4, a culture solution is carried in the first chamber 3, a first dissolved oxygen probe 5 is fixed on an inner wall of the first chamber 3, so that an upper layer of the semipermeable membrane carrier 2 has a plurality of lung epithelial cell layers 6, a lower layer of the semipermeable membrane carrier has a blood vessel endothelial cell layer, a culture solution is carried in the second chamber 4, a second dissolved oxygen probe 7 is fixed on an inner wall of the second chamber 4, a vent hole is opened at a top of the second chamber 4, a vent pipe 8 communicated with a set gas is fixed on the vent hole, both the first dissolved oxygen probe 5 and the second dissolved oxygen probe 7 are electrically connected with a controller 9, the controller 9 is electrically connected to the display screen 10.
The preset gas introduced through the ventilation pipe 8 is dispersed and sequentially enters the culture solution of the second chamber 4, the lung epithelial cell layer 6, the semipermeable membrane carrier 2, the vascular endothelial cell layer and the culture solution of the first chamber 3 to simulate the lung gas saturation process in the diving decompression disease process, the first dissolved oxygen probe 5 is used for detecting the content of dissolved oxygen in the culture solution of the first chamber 1 in real time as a first content value, the second dissolved oxygen probe 7 is used for detecting the content of dissolved oxygen in the culture solution of the second chamber 4 in real time as a second content value, the controller 9 is used for recording the received first content value and the corresponding time, the second content value and the corresponding time in real time, calculating the first content value of the lung gas real-time exchange rate at the current time/the second content value of the current time, and calculating the lung gas real-time dissolution rate (the current first content value in the culture solution of the first chamber-the first content value of the gas in the culture solution of the first chamber just entered) Value)/time between the current time and the time when gas just entered, the display screen 10 is used to display the real-time exchange rate of lung gas and the real-time dissolution rate of lung gas during the lung gas saturation process.
Stopping introducing set gas after the gas is saturated, dispersing the gas in the culture solution of the first cavity 3 out of the culture solution of the first cavity 3, the vascular endothelial cell layer, the semipermeable membrane carrier 2, the pulmonary epithelial cell layer 6 and the culture solution of the second cavity 4 in sequence to simulate the pulmonary gas desaturation process in the process of the diving decompression disease, wherein the first dissolved oxygen probe 5 is used for detecting the content of dissolved oxygen in the culture solution of the first cavity 3 in real time as a third content value, the second dissolved oxygen probe 7 is used for detecting the content of dissolved oxygen in the culture solution of the second cavity 4 in real time as a fourth content value, the controller 9 is used for recording the received third content value and corresponding time, the fourth content value and corresponding time in real time, calculating the real-time exchange rate of the pulmonary gas as the fourth content value of the current time/the third content value of the current time, and calculating the real-time desaturation rate of the pulmonary gas as the third content value when the gas in the culture solution of the first cavity is saturated Third content value of previous time)/time between current time and gas saturation time, the display screen 10 is used for displaying the lung gas real-time exchange rate and the lung gas real-time desaturation rate in the lung gas desaturation process.
The embodiment also provides a method for simulating and detecting lung gas exchange in a diving decompression sickness process, which is implemented by using the system for simulating and detecting lung gas exchange, and the method comprises the following steps:
as shown in fig. 2, the lung gas saturation process:
102, setting gas to diffuse into a culture solution of a second chamber, a lung epithelial cell layer, a semipermeable membrane carrier, a vascular endothelial cell layer and a culture solution of a first chamber in sequence so as to simulate a lung gas saturation process in the process of diving decompression sickness;
103, detecting the content of dissolved oxygen in the culture solution of the first chamber as a first content value in real time by the first dissolved oxygen probe, and detecting the content of dissolved oxygen in the culture solution of the second chamber as a second content value in real time by the second dissolved oxygen probe;
and 105, displaying the real-time lung gas exchange rate and the real-time lung gas dissolution rate in the lung gas saturation process by the display screen.
As shown in fig. 3, the lung gas desaturation process:
202, dispersing gas in the culture solution of the first cavity in sequence to obtain the culture solution of the first cavity, a vascular endothelial cell layer, a semipermeable membrane carrier, a pulmonary epithelial cell layer and the culture solution of the second cavity so as to simulate the desaturation process of lung gas in the process of diving decompression sickness;
and step 205, displaying the real-time lung gas exchange rate and the real-time lung gas desaturation rate in the lung gas desaturation process by the display screen.
While specific embodiments of the invention have been described above, it will be appreciated by those skilled in the art that these are by way of example only, and that the scope of the invention is defined by the appended claims. Various changes and modifications to these embodiments may be made by those skilled in the art without departing from the spirit and scope of the invention, and these changes and modifications are within the scope of the invention.
Claims (4)
1. A lung gas exchange simulation detection system in a diving decompression sickness process is characterized by comprising a sealed box body, wherein a semipermeable membrane carrier is fixed at the middle position in the sealed box body to divide the sealed box body into a first cavity and a second cavity from top to bottom, a culture solution is loaded in the first cavity, a first dissolved oxygen probe is fixed on the inner wall of the first cavity, so that the upper layer of the semipermeable membrane carrier is provided with a plurality of lung epithelial cell layers, the lower layer of the semipermeable membrane carrier is provided with an intravascular epithelial cell layer, the second cavity is loaded with the culture solution, a second dissolved oxygen probe is fixed on the inner wall of the second cavity, the top of the second cavity is provided with an air vent, the air vent is fixedly communicated with a vent pipe communicated with set gas, and the first dissolved oxygen probe and the second dissolved oxygen probe are both electrically connected with a controller;
the method comprises the steps that set gas introduced through the ventilation pipe is dispersed and sequentially enters culture solution of a second chamber, a lung epithelial cell layer, a semipermeable membrane carrier, a vascular endothelial cell layer and culture solution of the first chamber to simulate a lung gas saturation process in a diving decompression disease process, the first dissolved oxygen probe is used for detecting the content of dissolved oxygen in the culture solution of the first chamber in real time to serve as a first content value, the second dissolved oxygen probe is used for detecting the content of dissolved oxygen in the culture solution of the second chamber in real time to serve as a second content value, the controller is used for recording the received first content value, corresponding time, the second content value and corresponding time in real time, the real-time exchange rate of the lung gas is calculated as the first content value of the current time/the second content value of the current time, and the real-time dissolution rate of the lung gas is calculated as (the current first content value in the culture solution of the first chamber-the first content value of the gas just entering in the culture solution of the first chamber)/the current time and the current time The time between the first entry of gas;
stopping introducing the set gas after the gas is saturated, dispersing the gas in the culture solution of the first cavity in sequence to obtain the culture solution of the first cavity, a vascular endothelial cell layer, a semipermeable membrane carrier, a pulmonary epithelial cell layer and the culture solution of the second cavity so as to simulate the desaturation process of the lung gas in the process of the diving decompression disease, wherein the first dissolved oxygen probe is used for detecting the content of dissolved oxygen in the culture solution of the first cavity in real time as a third content value, the second dissolved oxygen probe is used for detecting the content of dissolved oxygen in the culture solution of the second cavity in real time as a fourth content value, the controller is used for recording the received third content value and corresponding time, the fourth content value and corresponding time in real time, calculating the real-time lung gas exchange rate which is the fourth content value of the current time/the third content value of the current time, and calculating the real-time lung gas desaturation rate which is the third content value of the current time when the gas in the culture solution of the first cavity is saturated Time between current time and gas saturation time.
2. The simulated pulmonary gas exchange detection system during a decompression sickness procedure according to claim 1, further comprising a display screen electrically connected to the controller, wherein the display screen is configured to display the real-time lung gas exchange rate and the real-time lung gas dissolution rate during a lung gas saturation procedure, and the real-time lung gas exchange rate and the real-time lung gas desaturation rate during a lung gas desaturation procedure.
3. A method for simulating lung gas exchange in a diving decompression sickness process, which is implemented by using the system for simulating lung gas exchange as claimed in claim 1, and comprises the following steps:
lung gas saturation procedure:
s11, introducing set gas into the vent pipe;
s12, setting gas to diffuse into the culture solution of the second chamber, the lung epithelial cell layer, the semi-permeable membrane carrier, the vascular endothelial cell layer and the culture solution of the first chamber in sequence so as to simulate the lung gas saturation process in the process of diving decompression sickness;
s13, detecting the content of dissolved oxygen in the culture solution of the first chamber as a first content value in real time by the first dissolved oxygen probe, and detecting the content of dissolved oxygen in the culture solution of the second chamber as a second content value in real time by the second dissolved oxygen probe;
s14, the controller records the received first content value and the corresponding time, the second content value and the corresponding time in real time, calculates the real-time exchange rate of the lung gas (the first content value in the culture solution in the first chamber-the first content value when the gas just enters in the culture solution in the first chamber)/the time between the current time and the time when the gas just enters in the first chamber);
lung gas desaturation process:
s21, stopping introducing the set gas after the gas is saturated;
s22, dispersing the gas in the culture solution of the first cavity in sequence to obtain the culture solution of the first cavity, the vascular endothelial cell layer, the semipermeable membrane carrier, the pulmonary epithelial cell layer and the culture solution of the second cavity so as to simulate the desaturation process of the lung gas in the process of diving decompression sickness;
s23, detecting the content of dissolved oxygen in the culture solution of the first chamber as a third content value in real time by the first dissolved oxygen probe, and detecting the content of dissolved oxygen in the culture solution of the second chamber as a fourth content value in real time by the second dissolved oxygen probe;
s24, the controller records the received third content value and the corresponding time, the received fourth content value and the corresponding time in real time, calculates the real-time lung gas exchange rate as the fourth content value of the current time/the third content value of the current time, and calculates the real-time lung gas desaturation rate as the third content value of the first chamber when the gas is saturated in the culture solution-the third content value of the current time/the time between the current time and the gas saturation time.
4. The simulated detection method of lung gas exchange during diving decompression sickness as claimed in claim 1, characterized in that the step S14 is followed by the steps of: s15, displaying the real-time exchange rate and the real-time dissolution rate of the lung gas in the lung gas saturation process by a display screen;
step S24 is followed by the steps of: and S25, displaying the real-time lung gas exchange rate and the real-time lung gas desaturation rate in the lung gas desaturation process by the display screen.
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