CN114113446A - Liquid chromatograph and method of use thereof - Google Patents
Liquid chromatograph and method of use thereof Download PDFInfo
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- CN114113446A CN114113446A CN202210103935.7A CN202210103935A CN114113446A CN 114113446 A CN114113446 A CN 114113446A CN 202210103935 A CN202210103935 A CN 202210103935A CN 114113446 A CN114113446 A CN 114113446A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/24—Automatic injection systems
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
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Abstract
The invention discloses a liquid chromatograph and a using method thereof, and the technical scheme is as follows: including solvent box, degasser, delivery pump, automatic model machine, post case and detection machine of advancing, solvent box, degasser, delivery pump, automatic model machine of advancing, post case and detection machine are piled up in proper order and are placed, and solvent box includes box body, groove box, baffle and bottom plate, groove box and box body top surface fixed connection, and the inside spacing lantern ring that is equipped with of groove box, baffle are located inside the box body, and baffle top fixedly connected with connecting rod, liquid chromatograph and application method beneficial effect are: through set up spacing lantern ring, storage assembly, drive assembly and drive assembly on the solvent box, place the solvent bottle in spacing lantern ring inboard, utilize storage assembly to twine the hose and accomodate to can drive spacing lantern ring during the operation and shift up, fix a position the solvent bottle with this, the security is stronger, can improve the stability of solvent bottle when accomodating the hose, and is more practical and convenient.
Description
Technical Field
The invention relates to the technical field of liquid chromatograph equipment, in particular to a liquid chromatograph and a using method thereof.
Background
High Performance Liquid Chromatography (HPLC) is developed rapidly by introducing gas chromatography theory in the late 60's of the 20 th century on the basis of classical liquid chromatography. The difference from classical liquid chromatography is that the filler particles are small and uniform. The small filling particles have high column efficiency but cause high resistance, and high pressure is needed to convey a mobile phase, so the small filling particles are also called high pressure liquid chromatography.
Current liquid chromatograph's solvent box is used for placing the solvent bottle, and the solvent bottle passes through the hose when using and is connected with other instruments, but a plurality of hoses are inconvenient to manage and the position is mixed and disorderly, and occupation space is big, and the operation solvent bottle directly places at solvent box internal stability not enough simultaneously, leads to the solvent bottle to topple over easily when the unexpected hose that touches during the operation or drop, safety inadequately.
Disclosure of Invention
Therefore, the liquid chromatograph and the using method thereof provided by the invention have the advantages that the solvent bottle is placed on the inner side of the limiting sleeve ring by arranging the limiting sleeve ring, the containing assembly, the transmission assembly and the driving assembly on the solvent box, the hose can be wound and contained by utilizing the containing assembly, and the limiting sleeve ring can be driven to move upwards during operation, so that the solvent bottle is positioned, the safety is stronger, the stability of the solvent bottle can be improved while the hose is contained, the liquid chromatograph is more practical and convenient, and the problems that the existing liquid chromatograph solvent bottle is not stable enough in placement and the hose on the solvent bottle occupies a large space in a messy manner are solved.
In order to achieve the above purpose, the invention provides the following technical scheme: the liquid chromatograph comprises a solvent box, a degasser, a conveying pump, an automatic sample feeder, a column box and a detector, wherein the solvent box, the degasser, the conveying pump, the automatic sample feeder, the column box and the detector are sequentially stacked, the solvent box comprises a box body, a groove box, a partition plate and a bottom plate, the groove box is fixedly connected with the top surface of the box body, a limiting lantern ring is arranged inside the groove box, the partition plate is positioned inside the box body, the top of the partition plate is fixedly connected with a connecting rod, the top end of the connecting rod is fixedly connected with the bottom wall of the groove box, the bottom plate is fixedly connected with the bottom of the box body, the outer wall of the box body is provided with a storage assembly, the bottom wall of the partition plate is provided with a transmission assembly, and the top of the partition plate is provided with a driving assembly;
the storage assembly comprises a short shaft and a bending baffle, the bending baffle is fixedly connected with the outer wall of the box body, the short shaft penetrates through the side wall of the box body and is movably connected with the side wall of the box body, a supporting rod is fixedly connected to one end, located outside the box body, of the short shaft, a circular plate is fixedly connected to one end, far away from the short shaft, of the supporting rod, and a storage circular ring is fixedly connected to one side, far away from the supporting rod, of the circular plate;
the transmission assembly comprises a transverse shaft, two mounting seats are sleeved on the transverse shaft, the transverse shaft is movably connected with the mounting seats, the two mounting seats are fixedly connected with the bottom wall of the partition plate, and one end of the transverse shaft is in transmission connection with the short shaft through a first gear set;
the driving assembly comprises a screw and a nut sleeve plate, the top end of the screw is movably connected with the bottom wall of the groove box, the bottom end of the screw penetrates through the partition plate and is movably connected with the partition plate, the bottom end of the screw is connected with the cross shaft through a second gear set, the nut sleeve plate is sleeved on the screw and is in threaded connection with the screw, the top end of the nut sleeve plate is fixedly connected with a guide rod, and the top end of the guide rod is hung and penetrates to the inside of the groove box and is fixedly connected with a limiting sleeve ring.
Preferably, the bending baffle comprises a limiting plate and a connecting plate, the limiting plate is distributed in parallel with the outer wall of the box body, the connecting plate is fixedly connected to the edge of the limiting plate, and the connecting plate is fixedly connected with the outer wall of the box body.
Preferably, the first gear set comprises a first gear and a second gear which are meshed with each other, the first gear is fixedly connected to the end of the stub shaft, and the second gear is fixedly connected to the end of the cross shaft.
Preferably, the second gear set comprises a first bevel gear and a second bevel gear which are meshed with each other, the first bevel gear is fixedly connected to the end of the transverse shaft, and the second bevel gear is fixedly connected to the bottom end of the screw rod.
Preferably, the limiting sleeve ring comprises a lower sleeve ring and an upper sleeve ring, a plurality of arc-shaped guide holes are formed in the edge of the upper sleeve ring, the lower sleeve ring is fixedly connected with the guide rod, a guide clamping rod is fixedly connected to the edge of the top of the lower sleeve ring and extends into the arc-shaped guide holes, and a plurality of special-shaped plates distributed in an annular array are arranged between the upper sleeve ring and the lower sleeve ring.
Preferably, a plurality of first chutes distributed in an annular array are formed in the top surface of the lower collar, a first sliding block is fixedly connected to the bottom of the special-shaped plate, and the first sliding block is slidably connected with the first chutes.
Preferably, a second sliding groove is formed in the top surface of the special-shaped plate, a plurality of second sliding blocks distributed in an annular array are fixedly connected to the bottom of the upper lantern ring, and the second sliding blocks are connected with the second sliding groove in a sliding mode.
Preferably, the top surface of the solvent box, the top surface of the degasser, the top surface of the delivery pump, the top surface of the automatic sample feeder, the top surface of the column box and the top surface of the detector are all provided with positioning grooves, and the bottom wall in each positioning groove is fixedly connected with a magnetic block.
Preferably, the bottom surface of the solvent box, the bottom surface of the degasser, the bottom surface of the delivery pump, the bottom surface of the automatic sample feeder, the bottom surface of the column box and the bottom surface of the detector are fixedly connected with metal positioning foot seats, and the metal positioning foot seats extend into the positioning grooves.
The invention also provides a using method of the liquid chromatograph, which comprises the following steps:
s1: firstly, placing a solvent bottle in a solvent box, enabling the solvent bottle to be positioned on the inner side of a limiting lantern ring, and then connecting one end of a hose with the solvent bottle and connecting the other end of the hose with other instruments;
s2: a section of length is reserved at one end, close to the solvent bottle, of the hose in the S1, the hose is wound on the containing ring by taking the section as a winding starting point, then the circular plate and the containing ring are pushed, the supporting rod can be driven to rotate by taking the short shaft as an axis, finally the circular plate and the containing ring can be rotated to the inner side of the bending baffle, the containing ring is attached to the inner wall of the bending baffle, and the circular plate and the containing ring keep the state under the action of gravity;
s3: when the short shaft rotates in S2, the short shaft can drive the first gear to rotate, the first gear can drive the second gear to rotate, the second gear can drive the transverse shaft to rotate, the transverse shaft drives the first bevel gear to rotate, the first bevel gear drives the second bevel gear to rotate, the second bevel gear can drive the screw to rotate, the screw can drive the nut sleeve plate to move upwards along the screw when rotating, the guide rod is driven to move upwards, the whole limiting sleeve ring can be pushed to move upwards when the guide rod moves upwards, and finally when the circular plate and the containing ring rotate to the inner wall of the bending baffle plate, the limiting sleeve ring just moves upwards to the middle of the solvent bottle and keeps stable, so that the solvent bottle can be positioned;
s4: the upper lantern ring of the limiting lantern ring in the S3 is rotated by applying force, the upper lantern ring can be relatively rotated under the guidance of the arc-shaped guide hole and the guide clamping rod, the second sliding block can be driven to move when the upper lantern ring rotates, and the circumferential tangential direction of the lantern ring above the second sliding block is driven to move, and the arrangement direction of the second sliding groove is different from the circumferential tangential direction of the upper lantern ring, so that the special-shaped plate can be pushed to move by the limitation of the second sliding groove when the second sliding block moves, and the first sliding block at the bottom of the special-shaped plate is limited by the first sliding groove on the lower lantern ring, so that the special-shaped plate and the first sliding block can only slide along the first sliding groove, finally, a plurality of special-shaped plates are relatively moved simultaneously, the edge of the special-shaped plate deviates to the center of the limiting lantern ring, the inner diameter of the limiting lantern ring can be reduced, and the inner diameter of the limiting lantern ring conforms to the outer diameter of the solvent bottle;
s5: the mobile phase in the solvent bottle in S1 is degassed by degasser and pumped into the system by transfer pump, the sample solution enters into the mobile phase by automatic sample feeder and is loaded into the chromatographic column (stationary phase) in the column box by the mobile phase, because each component in the sample solution has different distribution coefficient in two phases, when doing relative motion in two phases, through the distribution process of repeated adsorption-desorption, each component generates large difference in moving speed, and is separated into single components to flow out from the column in turn, when passing through the detector, the sample concentration is converted into electric signal and transmitted to the recorder, the data is printed out in the form of graph.
The embodiment of the invention has the following advantages:
1. the solvent bottle is placed on the inner side of the limiting sleeve ring by arranging the limiting sleeve ring, the accommodating assembly, the transmission assembly and the driving assembly on the solvent box, the hose can be wound and accommodated by the accommodating assembly, and the limiting sleeve ring can be driven to move upwards during operation, so that the solvent bottle is positioned, the safety is higher, the stability of the solvent bottle can be improved while the hose is accommodated, and the solvent bottle is more practical and convenient;
2. the limiting lantern ring is arranged into the upper lantern ring, the lower lantern ring and the plurality of special-shaped plates, the upper lantern ring is rotated to drive the plurality of special-shaped plates to move at the same time, so that the inner diameter of the limiting lantern ring can be adjusted, the limiting lantern ring is suitable for solvent bottles with different specifications, and the operation is convenient and simple;
3. through setting up constant head tank, magnetic path and metal location foot rest, can fix a position and adsorb fixedly to adjacent machine that stacks, improve the stability of equipment, it is safer.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It should be apparent that the drawings in the following description are merely exemplary, and that other embodiments can be derived from the drawings provided by those of ordinary skill in the art without inventive effort.
The structures, ratios, sizes, and the like shown in the present specification are only used for matching with the contents disclosed in the specification, so as to be understood and read by those skilled in the art, and are not used to limit the conditions that the present invention can be implemented, so that the present invention has no technical significance, and any structural modifications, changes in the ratio relationship, or adjustments of the sizes, without affecting the effects and the achievable by the present invention, should still fall within the range that the technical contents disclosed in the present invention can cover.
FIG. 1 is a schematic view of the overall structure provided by the present invention;
FIG. 2 is a schematic view of the construction of a solvent cartridge provided by the present invention;
FIG. 3 is a schematic structural view of a separator according to the present invention;
FIG. 4 is a cross-sectional effect view of a solvent cartridge provided by the present invention;
FIG. 5 is an exploded top view of the stop collar of the present invention;
fig. 6 is an exploded bottom view of the stop collar of the present invention.
In the figure: the automatic sample feeder comprises a solvent box 1, a box 110, a groove box 120, a partition 130, a bottom plate 140, a degasser 2, a delivery pump 3, an automatic sample feeder 4, a column box 5, a detector 6, a position limiting lantern ring 7, a lower lantern ring 71, an upper lantern ring 72, an arc-shaped guide hole 73, a guide clamping rod 74, a special-shaped plate 75, a first sliding groove 76, a first sliding block 77, a second sliding groove 78, a second sliding block 79, a connecting rod 8, a short shaft 9, a bent baffle 10, a limiting plate 101, a connecting plate 102, a support rod 11, a circular plate 12, a storage circular ring 13, a transverse shaft 14, a mounting seat 15, a first gear set 16, a first gear 161, a second gear 162, a screw rod 17, a nut sleeve plate 18, a second gear set 19, a gear set 191, a first bevel gear, a second bevel gear 192, a guide rod 20, a positioning groove 21, a magnetic block 22 and a metal positioning footstand 23.
Detailed Description
The present invention is described in terms of particular embodiments, other advantages and features of the invention will become apparent to those skilled in the art from the following disclosure, and it is to be understood that the described embodiments are merely exemplary of the invention and that it is not intended to limit the invention to the particular embodiments disclosed. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-6, the liquid chromatograph provided by the present invention comprises a solvent box 1, a degasser 2, a delivery pump 3, an automatic sample feeder 4, a column box 5 and a detector 6, wherein the solvent box 1, the degasser 2, the delivery pump 3, the automatic sample feeder 4, the column box 5 and the detector 6 are sequentially stacked, the solvent box 1 comprises a box body 110, a slot box 120, a partition 130 and a bottom plate 140, the slot box 120 is fixedly connected with the top surface of the box body 110, a limiting collar 7 is arranged inside the slot box 120, the partition 130 is positioned inside the box body 110, the top of the partition 130 is fixedly connected with a connecting rod 8, the top of the connecting rod 8 is fixedly connected with the bottom wall of the slot box 120, the bottom plate 140 is fixedly connected with the bottom of the box body 110, the outer wall of the box body 110 is provided with a receiving assembly, the bottom wall of the partition 130 is provided with a transmission assembly, and the top of the partition 130 is provided with a driving assembly;
the containing assembly comprises a short shaft 9 and a bent baffle 10, the bent baffle 10 is fixedly connected with the outer wall of the box body 110, the short shaft 9 penetrates through the side wall of the box body 110 and is movably connected with the side wall of the box body 110, one end, located outside the box body 110, of the short shaft 9 is fixedly connected with a supporting rod 11, one end, far away from the short shaft 9, of the supporting rod 11 is fixedly connected with a circular plate 12, and one side, far away from the supporting rod 11, of the circular plate 12 is fixedly connected with a containing circular ring 13;
the transmission assembly comprises a transverse shaft 14, two mounting seats 15 are sleeved on the transverse shaft 14, the transverse shaft 14 is movably connected with the mounting seats 15, the two mounting seats 15 are fixedly connected with the bottom wall of the partition plate 130, and one end of the transverse shaft 14 is in transmission connection with the short shaft 9 through a first gear set 16;
the driving assembly comprises a screw rod 17 and a nut sleeve plate 18, the top end of the screw rod 17 is movably connected with the bottom wall of the groove box 120, the bottom end of the screw rod 17 penetrates through the partition plate 130 and is movably connected with the partition plate 130, the bottom end of the screw rod 17 is connected with the transverse shaft 14 through a second gear set 19, the nut sleeve plate 18 is sleeved on the screw rod 17 and is in threaded connection with the screw rod 17, the top end of the nut sleeve plate 18 is fixedly connected with a guide rod 20, and the top end of the guide rod 20 is hung and penetrated into the groove box 120 and is fixedly connected with a limiting lantern ring 7;
in this embodiment, the internal structures of the degasser 2, the delivery pump 3, the automatic sample feeder 4, the column box 5 and the detector 6 are the same as those of the prior art, and will not be described herein, in practical application, the number of the limiting collar 7, the receiving assembly, the transmission assembly and the driving assembly is at least four, the circular plate 12 and the receiving collar 13 are located at the top of the bending baffle 10 in the initial state, before working, the solvent bottle is first placed in the solvent box 1 and is located at the inner side of the limiting collar 7, then one end of the hose is connected with the solvent bottle, the other end is connected with other instruments, at this time, if the length of the hose is longer, the hose is wound on the receiving collar 13, then the circular plate 12 and the receiving collar 13 are pushed, the supporting rod 11 can be driven to rotate by taking the short shaft 9 as the axis, finally the circular plate 12 and the receiving collar 13 can be rotated to the inner side of the bending baffle 10, at this time, the inner wall of the bending baffle 10 of the receiving collar 13 is stored, the hose can be prevented from falling off from the containing ring 13, meanwhile, the circular plate 12 and the containing ring 13 keep the state under the action of gravity, the containing management of the hose is realized, the short shaft 9 can drive the transverse shaft 14 to rotate through the first gear set 16 while the short shaft 9 rotates, the transverse shaft 14 can drive the screw 17 to rotate through the second gear set 19 while rotating, the screw 17 can drive the nut sleeve plate 18 to move upwards along the screw 17 while rotating, the guide rod 20 is driven to move upwards, the whole limiting sleeve ring 7 can be pushed to move upwards when the guide rod 20 moves upwards, and finally, when the circular plate 12 and the containing ring 13 rotate to the inner wall of the bent baffle plate 10, the limiting sleeve ring 7 just moves upwards to the middle part of the solvent bottle and keeps stable, so that the solvent bottle can be positioned, and the stability of the solvent bottle is improved;
wherein, in order to realize the purpose of fixed baffle 10 of buckling, this device adopts following technical scheme to realize: the bending baffle 10 comprises a limiting plate 101 and a connecting plate 102, the limiting plate 101 is distributed in parallel with the outer wall of the box body 110, the connecting plate 102 is fixedly connected to the edge of the limiting plate 101, the connecting plate 102 is fixedly connected with the outer wall of the box body 110, and the bending baffle 10 can limit the hose sleeved on the containing ring 13 to avoid separation of the hose by arranging the bending baffle 10;
in order to realize the purpose of driving the transverse shaft 14 to rotate, the device adopts the following technical scheme: the first gear set 16 comprises a first gear 161 and a second gear 162 which are meshed with each other, the first gear 161 is fixedly connected to the end of the stub shaft 9, the second gear 162 is fixedly connected to the end of the cross shaft 14, the diameter of the first gear 161 is larger than that of the second gear 162 in actual application, and the first gear 161 can drive the second gear 162 to rotate for multiple turns when rotating for half a turn;
wherein, in order to realize the purpose of driving the screw 17 to rotate, the device adopts the following technical scheme: the second gear set 19 comprises a first bevel gear 191 and a second bevel gear 192 which are meshed with each other, the first bevel gear 191 is fixedly connected to the end of the cross shaft 14, the second bevel gear 192 is fixedly connected to the bottom end of the screw 17, and the cross shaft 14 can drive the first bevel gear 191 to rotate when rotating, so as to drive the second bevel gear 192 and the screw 17 to rotate;
wherein, in order to realize the purpose of adjusting the internal diameter of the limiting lantern ring 7, the device adopts the following technical scheme: the limiting lantern ring 7 comprises a lower lantern ring 71 and an upper lantern ring 72, a plurality of arc-shaped guide holes 73 are formed in the edge of the upper lantern ring 72, the lower lantern ring 71 is fixedly connected with the guide rods 20, a guide clamping rod 74 is fixedly connected to the top edge of the lower lantern ring 71, the guide clamping rod 74 extends into the arc-shaped guide holes 73, a plurality of special-shaped plates 75 distributed in an annular array are arranged between the upper lantern ring 72 and the lower lantern ring 71, a plurality of first sliding grooves 76 distributed in an annular array are formed in the top surface of the lower lantern ring 71, a first sliding block 77 is fixedly connected to the bottom of the special-shaped plate 75, the first sliding block 77 is in sliding connection with the first sliding groove 76, a second sliding groove 78 is formed in the top surface of the special-shaped plate 75, a plurality of second sliding blocks 79 distributed in an annular array are fixedly connected to the bottom of the upper lantern ring 72, the second sliding block 79 is in sliding connection with the second sliding groove 78, in practical application, the number of the special-shaped plates 75, the first sliding grooves 76 and the second sliding blocks 79 is preferably set to 8, the number of the guide clamping rods 74 and the number of the arc-shaped guide holes 73 are preferably four, the guide clamping rods 74 are tightly attached to the arc-shaped guide holes 73 in an initial state, the upper lantern ring 72 can be prevented from rotating randomly by friction force, and the upper lantern ring 72 can be driven to rotate by force application; the upper lantern ring 72 can relatively rotate under the guidance of the arc-shaped guide hole 73 and the guide clamping rod 74, the upper lantern ring 72 can drive the second sliding block 79 to move when rotating, the second sliding block 79 can push the special-shaped plates 75 to move through the limit of the second sliding groove 78 when moving, so that the special-shaped plates 75 and the first sliding block 77 slide along the first sliding groove 76, finally, the plurality of special-shaped plates 75 relatively move simultaneously, the edges of the special-shaped plates 75 deviate to the center of the limit lantern ring 7, and the inner diameter of the limit lantern ring 7 can be reduced, so that the solvent bottle with a smaller diameter is met;
wherein, in order to realize the purpose that improves the stability after each instrument stacks, this device adopts following technical scheme to realize: top surface of solvent box 1, 2 top surfaces of degasser, 3 top surfaces of delivery pump, 4 top surfaces of automatic sample feeder, constant head tank 21 has all been seted up to 5 top surfaces of column box and 6 top surfaces of detection machine, wall fixedly connected with magnetic path 22 in constant head tank 21, the bottom surface of solvent box 1, 2 bottom surfaces of degasser, 3 bottom surfaces of delivery pump, 4 bottom surfaces of automatic sample feeder, 5 bottom surfaces of column box and the equal fixedly connected with metal location foot stool 23 in the 6 bottom surfaces of detection machine, metal location foot stool 23 extends to in the constant head tank 21, metal location foot stool 23 is preferred to set up to the steel material, can adsorb fixedly with the magnetic path 22 in the constant head tank 21, with this stability improvement, it is safer.
The using process of the invention is as follows: when the device is used, a solvent bottle is firstly placed in a solvent box 1 and is positioned at the inner side of a limiting lantern ring 7, then one end of a hose is connected with the solvent bottle, and the other end of the hose is connected with other instruments, at the moment, if the length of the hose is longer, a section of length is reserved at one end, close to the solvent bottle, of the hose, the hose is wound on a containing ring 13 by taking the section as a winding starting point, then the circular plate 12 and the containing ring 13 are pushed, a supporting rod 11 can be driven to rotate by taking a short shaft 9 as an axis, finally, the circular plate 12 and the containing ring 13 can be rotated to the inner side of a bending baffle 10, at the moment, the containing ring 13 is close to the inner wall of the bending baffle 10, the hose can be prevented from falling off from the containing ring 13, and the circular plate 12 and the containing ring 13 keep the state under the action of gravity, so that the containing management of the hose is realized;
when the short shaft 9 rotates, the short shaft 9 can drive the first gear 161 to rotate, the first gear 161 can drive the second gear 162 to rotate, the second gear 162 can drive the transverse shaft 14 to rotate when rotating, the transverse shaft 14 drives the first bevel gear 191 to rotate, the first bevel gear 191 drives the second bevel gear 192 to rotate, the second bevel gear 192 can drive the screw 17 to rotate when rotating, the screw 17 can drive the nut sleeve plate 18 to move upwards along the screw 17 when rotating, the guide rod 20 is driven to move upwards, the whole limiting sleeve ring 7 can be pushed to move upwards when the guide rod 20 moves upwards, finally when the circular plate 12 and the accommodating ring 13 rotate to the inner wall of the bent baffle plate 10, the limiting sleeve ring 7 just moves upwards to the middle of the solvent bottle and keeps stable, so that the solvent bottle can be positioned, and the stability of the solvent bottle is improved;
when the diameter of the solvent bottle is smaller, the upper lantern ring 72 can be rotated by applying force, the upper lantern ring 72 can be relatively rotated under the guidance of the arc-shaped guide hole 73 and the guide clamping rod 74, the upper lantern ring 72 can drive the second sliding block 79 to move when rotating, the second sliding block 79 can push the special-shaped plate 75 to move through the limit of the second sliding groove 78 when moving, so that the special-shaped plate 75 and the first sliding block 77 slide along the first sliding groove 76, finally, the plurality of special-shaped plates 75 simultaneously move relatively, the edge of the special-shaped plate 75 shifts to the center of the limit lantern ring 7, and the inner diameter of the limit lantern ring 7 can be reduced, so that the solvent bottle with the smaller diameter is met.
The invention also provides a using method of the liquid chromatograph, which comprises the following steps:
s1: firstly, a solvent bottle is placed in a solvent box 1 and is positioned at the inner side of a limiting lantern ring 7, and then one end of a hose is connected with the solvent bottle, and the other end of the hose is connected with other instruments;
s2: a section of length is reserved at one end, close to the solvent bottle, of the hose in the step S1, the hose is wound on the containing ring 13 by taking the section as a winding starting point, then the circular plate 12 and the containing ring 13 are pushed, the supporting rod 11 can be driven to rotate by taking the short shaft 9 as an axis, finally the circular plate 12 and the containing ring 13 can be rotated to the inner side of the bending baffle plate 10, the containing ring 13 is attached to the inner wall of the bending baffle plate 10, and meanwhile the circular plate 12 and the containing ring 13 keep the state under the action of gravity;
s3: when the short shaft 9 rotates in S2, the short shaft 9 can drive the first gear 161 to rotate, the first gear 161 can drive the second gear 162 to rotate, the second gear 162 can drive the transverse shaft 14 to rotate when rotating, the transverse shaft 14 drives the first bevel gear 191 to rotate, the first bevel gear 191 drives the second bevel gear 192 to rotate, the second bevel gear 192 can drive the screw 17 to rotate when rotating, the screw 17 can drive the nut sleeve plate 18 to move upwards along the screw 17 when rotating, and simultaneously drive the guide rod 20 to move upwards, the guide rod 20 can drive the whole position-limiting sleeve ring 7 to move upwards when moving upwards, and finally when the circular plate 12 and the accommodating ring 13 rotate to the inner wall of the bending baffle plate 10, the position-limiting sleeve ring 7 just moves upwards to the middle part of the solvent bottle and keeps stable, so as to position the solvent bottle;
s4: the upper lantern ring 72 of the position limiting lantern ring 7 in the S3 is forced to rotate, the upper lantern ring 72 can relatively rotate under the guidance of the arc-shaped guide hole 73 and the guide clamping rod 74, the second sliding block 79 can be driven to move when the upper lantern ring 72 rotates, and drives the second sliding block 79 to move in the circumferential tangential direction of the upper lantern ring 72, and because the arrangement direction of the second sliding groove 78 is different from the circumferential tangential direction of the upper lantern ring 72, the second sliding block 79 can push the shaped plate 75 to move through the limit of the second sliding slot 78 when moving, and because the first slide block 77 at the bottom of the profiled plate 75 is limited by the first slide slot 76 on the lower collar 71, therefore, the special-shaped plates 75 and the first sliding blocks 77 can only slide along the first sliding grooves 76, and finally, the special-shaped plates 75 move relatively at the same time, the edges of the special-shaped plates 75 deviate towards the center of the limiting lantern ring 7, so that the inner diameter of the limiting lantern ring 7 can be reduced, and the inner diameter of the limiting lantern ring 7 conforms to the outer diameter of a solvent bottle;
s5: the mobile phase in the solvent bottle in S1 is degassed by degasser 2, and then pumped into the system by transfer pump 3, the sample solution enters into the mobile phase by automatic sampler 4, and is loaded into the stationary phase of chromatographic column in column box 5 by the mobile phase, because each component in the sample solution has different distribution coefficient in two phases, when doing relative motion in two phases, through the distribution process of repeated adsorption-desorption, each component generates large difference in moving speed, and is separated into single components to flow out from the column in turn, when passing through detector 6, the sample concentration is converted into electric signal to be transmitted to the recorder, and the data is printed out in the form of graph.
The above is only a preferred embodiment of the present invention, and any person skilled in the art may modify the present invention or modify it into an equivalent technical solution by using the technical solution described above. Therefore, any simple modifications or equivalent substitutions made in accordance with the technical solution of the present invention are within the scope of the claims of the present invention.
Claims (10)
1. Liquid chromatograph, including solvent box (1), degasser (2), delivery pump (3), automatic sample feeder (4), column box (5) and detection machine (6), its characterized in that: the device comprises a solvent box (1), a degasser (2), a delivery pump (3), an automatic sample feeder (4), a column box (5) and a detection machine (6), wherein the solvent box (1) is sequentially stacked, the solvent box (1) comprises a box body (110), a groove box (120), a partition plate (130) and a bottom plate (140), the groove box (120) is fixedly connected with the top surface of the box body (110), a limiting lantern ring (7) is arranged inside the groove box (120), the partition plate (130) is positioned inside the box body (110), the top of the partition plate (130) is fixedly connected with a connecting rod (8), the top end of the connecting rod (8) is fixedly connected with the bottom wall of the groove box (120), the bottom plate (140) is fixedly connected with the bottom of the box body (110), a containing assembly is arranged on the outer wall of the box body (110), a transmission assembly is arranged on the bottom wall of the partition plate (130), and a driving assembly is arranged on the top of the partition plate (130);
the containing assembly comprises a short shaft (9) and a bent baffle (10), the bent baffle (10) is fixedly connected with the outer wall of the box body (110), the short shaft (9) penetrates through the side wall of the box body (110) and is movably connected with the side wall of the box body (110), one end, located outside the box body (110), of the short shaft (9) is fixedly connected with a supporting rod (11), one end, far away from the short shaft (9), of the supporting rod (11) is fixedly connected with a circular plate (12), and one side, far away from the supporting rod (11), of the circular plate (12) is fixedly connected with a containing circular ring (13);
the transmission assembly comprises a transverse shaft (14), two mounting seats (15) are sleeved on the transverse shaft (14), the transverse shaft (14) is movably connected with the mounting seats (15), the two mounting seats (15) are fixedly connected with the bottom wall of the partition plate (130), and one end of the transverse shaft (14) is in transmission connection with the short shaft (9) through a first gear set (16);
the driving assembly comprises a screw rod (17) and a nut sleeve plate (18), the top end of the screw rod (17) is movably connected with the bottom wall of the groove box (120), the bottom end of the screw rod (17) penetrates through the partition plate (130) and is movably connected with the partition plate (130), the bottom end of the screw rod (17) is connected with the transverse shaft (14) through a second gear set (19), the nut sleeve plate (18) is sleeved on the screw rod (17) and is in threaded connection with the screw rod (17), the top end of the nut sleeve plate (18) is fixedly connected with a guide rod (20), and the top end of the guide rod (20) is hung and penetrates to the inside of the groove box (120) and is fixedly connected with a limiting sleeve ring (7).
2. The liquid chromatograph of claim 1, wherein: the bending baffle (10) comprises a limiting plate (101) and a connecting plate (102), the limiting plate (101) and the outer wall of the box body (110) are distributed in parallel, the connecting plate (102) is fixedly connected to the edge of the limiting plate (101), and the connecting plate (102) is fixedly connected with the outer wall of the box body (110).
3. The liquid chromatograph of claim 1, wherein: the first gear set (16) comprises a first gear (161) and a second gear (162) which are meshed with each other, the first gear (161) is fixedly connected to the end of the stub shaft (9), and the second gear (162) is fixedly connected to the end of the transverse shaft (14).
4. The liquid chromatograph of claim 1, wherein: the second gear set (19) comprises a first bevel gear (191) and a second bevel gear (192) which are meshed with each other, the first bevel gear (191) is fixedly connected to the end of the transverse shaft (14), and the second bevel gear (192) is fixedly connected to the bottom end of the screw (17).
5. The liquid chromatograph of claim 1, wherein: spacing lantern ring (7) are including lower lantern ring (71) and last lantern ring (72), a plurality of arc guiding holes (73) have been seted up at last lantern ring (72) edge, lower lantern ring (71) and guide bar (20) fixed connection, lower lantern ring (71) top edge fixedly connected with direction kelly (74), direction kelly (74) extend to in arc guiding holes (73), go up lantern ring (72) and be equipped with dysmorphism board (75) that a plurality of annular arrays distribute down between lantern ring (71).
6. The liquid chromatograph of claim 5, wherein: a plurality of first chutes (76) distributed in an annular array are formed in the top surface of the lower lantern ring (71), first sliding blocks (77) are fixedly connected to the bottom of the special-shaped plate (75), and the first sliding blocks (77) are connected with the first chutes (76) in a sliding mode.
7. The liquid chromatograph of claim 5, wherein: second spout (78) have been seted up to dysmorphism board (75) top surface, go up lantern ring (72) bottom fixedly connected with a plurality of annular array distribution's second slider (79), second slider (79) and second spout (78) sliding connection.
8. The liquid chromatograph of claim 1, wherein: positioning grooves (21) are formed in the top surface of the solvent box (1), the top surface of the degasser (2), the top surface of the delivery pump (3), the top surface of the automatic sample feeder (4), the top surface of the column box (5) and the top surface of the detector (6), and magnetic blocks (22) are fixedly connected to the inner bottom wall of each positioning groove (21).
9. The liquid chromatograph of claim 8, wherein: the bottom surface of the solvent box (1), the bottom surface of the degasser (2), the bottom surface of the delivery pump (3), the bottom surface of the automatic sample feeder (4), the bottom surface of the column box (5) and the bottom surface of the detector (6) are fixedly connected with metal positioning foot seats (23), and the metal positioning foot seats (23) extend into the positioning grooves (21).
10. The use method of the liquid chromatograph is characterized in that: the method comprises the following steps:
s1: firstly, a solvent bottle is placed in a solvent box (1) and is positioned at the inner side of a limiting lantern ring (7), and then one end of a hose is connected with the solvent bottle, and the other end of the hose is connected with other instruments;
s2: a section of hose in S1 is reserved at one end close to the solvent bottle, and is wound on the containing ring (13) by taking the section as a winding starting point, then the circular plate (12) and the containing ring (13) are pushed, the support rod (11) can be driven to rotate by taking the short shaft (9) as an axis, finally the circular plate (12) and the containing ring (13) can be rotated to the inner side of the bending baffle (10), at the moment, the containing ring (13) is attached to the inner wall of the bending baffle (10), and meanwhile, the circular plate (12) and the containing ring (13) keep the state under the action of gravity;
s3: in S2, when the short shaft (9) rotates, the short shaft (9) can drive the first gear (161) to rotate, the first gear (161) can drive the second gear (162) to rotate, the second gear (162) can drive the transverse shaft (14) to rotate, the transverse shaft (14) drives the first bevel gear (191) to rotate, the first bevel gear (191) drives the second bevel gear (192) to rotate, the second bevel gear (192) can drive the screw (17) to rotate, the screw (17) can drive the nut sleeve plate (18) to move upwards along the screw (17) when rotating, and simultaneously drives the guide rod (20) to move upwards, the guide rod (20) can drive the whole limiting sleeve ring (7) to move upwards when moving upwards, and finally when the circular plate (12) and the containing circular ring (13) rotate to the inner wall of the bending baffle (10), the limiting sleeve ring (7) just moves upwards to the middle part of the solvent bottle and keeps stable, thus, the solvent bottle can be positioned;
s4: the upper lantern ring (72) of the limiting lantern ring (7) in the S3 is forced to rotate, the upper lantern ring (72) can rotate relatively under the guidance of the arc-shaped guide hole (73) and the guide clamping rod (74), the upper lantern ring (72) can drive the second sliding block (79) to move when rotating, and drive the second sliding block (79) to move in the circumferential tangential direction of the upper lantern ring (72), and because the arrangement direction of the second sliding groove (78) is different from the circumferential tangential direction of the upper lantern ring (72), the second sliding block (79) can push the special-shaped plate (75) to move through the limiting of the second sliding groove (78) when moving, and because the first sliding block (77) at the bottom of the special-shaped plate (75) is limited by the first sliding groove (76) on the lower lantern ring (71), the special-shaped plate (75) and the first sliding block (77) can only slide along the first sliding groove (76), and finally the special-shaped plates (75) can move relatively, the edge of the special-shaped plate (75) deviates to the center of the limiting sleeve ring (7), so that the inner diameter of the limiting sleeve ring (7) can be reduced, and the inner diameter of the limiting sleeve ring (7) conforms to the outer diameter of the solvent bottle;
s5: s1, the mobile phase in the solvent bottle is degassed by a degasser (2), and then is pumped into the system by a delivery pump (3), the sample solution enters the mobile phase by an automatic sample feeder (4), and is loaded into a chromatographic column (stationary phase) in a column box (5) by the mobile phase, because each component in the sample solution has different distribution coefficients in two phases, when the two phases move relatively, through the distribution process of repeated adsorption-desorption, each component generates large difference in moving speed, is separated into single components and flows out from the column in sequence, when the sample solution passes through a detector (6), the sample concentration is converted into an electric signal and is transmitted to a recorder, and data is printed in a graph form.
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| CN114113446B (en) | 2022-04-12 |
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