CN114113058A - Sample collection and purification integrated detection card and use method thereof - Google Patents
Sample collection and purification integrated detection card and use method thereof Download PDFInfo
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- CN114113058A CN114113058A CN202111381861.5A CN202111381861A CN114113058A CN 114113058 A CN114113058 A CN 114113058A CN 202111381861 A CN202111381861 A CN 202111381861A CN 114113058 A CN114113058 A CN 114113058A
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- 238000001514 detection method Methods 0.000 title claims abstract description 80
- 238000000746 purification Methods 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 15
- 210000003296 saliva Anatomy 0.000 claims abstract description 88
- 238000012360 testing method Methods 0.000 claims abstract description 60
- 238000011161 development Methods 0.000 claims description 24
- 210000002700 urine Anatomy 0.000 claims description 21
- 239000008280 blood Substances 0.000 claims description 19
- 210000004369 blood Anatomy 0.000 claims description 19
- 238000001914 filtration Methods 0.000 claims description 18
- 238000004587 chromatography analysis Methods 0.000 claims description 6
- 238000001125 extrusion Methods 0.000 claims description 4
- 238000010521 absorption reaction Methods 0.000 claims description 3
- 230000017531 blood circulation Effects 0.000 claims description 2
- 238000004891 communication Methods 0.000 claims 1
- 210000001124 body fluid Anatomy 0.000 abstract description 2
- 239000010839 body fluid Substances 0.000 abstract description 2
- 230000000694 effects Effects 0.000 description 4
- 238000007689 inspection Methods 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 208000035473 Communicable disease Diseases 0.000 description 1
- 208000018522 Gastrointestinal disease Diseases 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 206010047700 Vomiting Diseases 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000003317 immunochromatography Methods 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 208000023504 respiratory system disease Diseases 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
- A61B10/0045—Devices for taking samples of body liquids
- A61B10/0051—Devices for taking samples of body liquids for taking saliva or sputum samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
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- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- General Physics & Mathematics (AREA)
- Hematology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Pulmonology (AREA)
- Plasma & Fusion (AREA)
- Heart & Thoracic Surgery (AREA)
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- Animal Behavior & Ethology (AREA)
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Abstract
The invention relates to a sample collection and purification integrated detection card and a use method thereof, wherein the detection card comprises a detection card body, the upper part of the detection card body is provided with an upper cover, the lower part of the detection card body is provided with a lower cover, two sides of the detection card body are respectively provided with an accommodating cavity, and the accommodating cavity is opened at the upper end of the detection card body; a saliva pad is arranged in the containing cavity, the upper end of the saliva pad extends out of the containing cavity, and the lower end of the saliva pad extends to the bottom of the containing cavity; the test card comprises a test card body and is characterized in that a placing cavity is formed in the middle of the test card body, a test strip is arranged in the placing cavity, and the test strip extends downwards out of the placing cavity; the portable detection card for body fluid detection has the advantages that the operation is simple, the sample collection and purification integrated detection device does not need to be kept in a horizontal state during sample collection, the operation is not limited, color indication appears after the sample collection is finished, the sample can be taken out from the mouth in a prompting way, the sample is collected and filtered from top to bottom, the sample is prevented from rushing out of a detection window, the interference of the sample can be furthest reduced to the minimum, and the stability of the product performance is ensured.
Description
Technical Field
The invention belongs to the technical field of detection, and particularly relates to a sample collection and purification integrated detection card and a use method thereof.
Background
In clinical detection, saliva is used more and more widely as a safe, noninvasive, simple and easily-obtained sample in the fields of disease diagnosis, drug detection and the like, and products capable of self-detection gradually become a new trend along with the attention of people on self health. The product developed by taking immunochromatography as a technical platform can be used for self-detection screening of respiratory diseases, gastrointestinal diseases and infectious diseases and plays a role in the aspect of auxiliary clinical diagnosis.
The product for self-detection needs to be simple to operate, short in detection time and easy to judge the result, and firstly, the source of the sample is convenient, saliva, urine and blood can be used as detection samples, and the saliva sample is preferred. Saliva sample accessible is directly vomited, sampling stick scraping is gathered, is sucked saliva pad mode such as and is obtained, and there is the difference in different people's saliva sample, and tangible component content is different in the saliva, and some samples are clear and transparent, and some samples are thick then yellow, and saliva sample's matrix effect has very big influence to the testing result. Saliva can be purified by centrifugation, water bath, lysis of lysate and the like, and the matrix effect is reduced, but the method either requires equipment or has complicated steps, and is not convenient enough for self-detection.
The existing one-step saliva detection card on the market mostly uses a buccal saliva pad to obtain a sample, and the sample is chromatographically upward through the sample pad to complete the test, the method does not need equipment, and the steps are relatively simple, but still has the following defects: 1. the saliva pad is arranged below the detection device, so that when the saliva pad is sucked, the detection device is kept in a horizontal state, the labor is relatively wasted, the saliva pad needs to be placed under the tongue or kept flat manually, and the use feeling is poor; 2. whether the sample is collected or not is not obviously indicated, and the test fails due to the fact that the sample amount is too large or too small; 3. the sample is collected and purified only through the saliva pad and the sample pad, and the sample with a large amount of visible components cannot be completely purified; 4. in use, the sample risks being flushed out of the inspection window if the inspection device is not kept horizontal.
Disclosure of Invention
The invention aims to provide a detection card which is small in size, convenient to carry, quick and simple to use, safe and convenient, can be used for self-detection and can be used for detecting various body fluids, and a using method thereof.
In order to solve the technical problem, the invention discloses a sample collection and purification integrated detection card which comprises a detection card body, wherein an upper cover is arranged at the upper part of the detection card body, a lower cover is arranged at the lower part of the detection card body, and the upper cover and the lower cover adopt interfaces with the same size, so that the upper cover and the lower cover can be interchanged;
accommodating cavities are respectively arranged on two sides of the detection card body, the accommodating cavities are parallel to the length direction of the detection card body, and the accommodating cavities are opened at the upper end of the detection card body; the bottom of the accommodating cavity is provided with a plurality of through holes;
a saliva pad is arranged in the containing cavity, the upper end of the saliva pad extends out of the containing cavity, and the lower end of the saliva pad extends to the bottom of the containing cavity;
the test card comprises a test card body and is characterized in that a placing cavity with an opening at the lower end of the test card body is formed in the middle of the test card body, a test strip is arranged in the placing cavity, the test strip extends out of the placing cavity downwards, and an observation window for observing the test result of the test strip is processed on the test card body;
the length of the test strip extending out of the placing cavity is not less than 2/3 of the distance between the top of the upper cover and the middle part of the upper end of the detection card body.
Preferably, a filter paper is arranged between the bottom of the containing cavity and the saliva pad.
Preferably, the filter paper comprises a color development sheet for preliminary filtration and showing saliva absorption and a dense filter paper for filtering saliva, wherein the color development sheet is arranged on the dense filter paper.
Preferably, a transparent window is arranged at the position, corresponding to the color development piece, on the detection card body.
Preferably, protruding extension parts are arranged on two sides of the upper end of the detection card body, and the accommodating cavity is opened at the top end of the extension parts.
Preferably, a pair of pillars which can extend into the accommodating cavity to press the saliva pad are arranged at the position, corresponding to the accommodating cavity, of the bottom of the inner side of the lower cover.
Preferably, the lower cover is provided with a press rod capable of sliding up and down, and the press rod penetrates through the bottom of the lower cover and is connected with the pair of columns.
Preferably, a groove is formed in the top of the inner side of the upper cover and corresponds to the test strip, and when the upper cover is placed at the position of the lower cover, the lower end of the test strip extends into the groove.
The utility model provides a use method of sample collection purification integral type detection card, when adopting saliva sample detection, pull out the upper cover and replace it to the lower cover position, hold saliva pad and collect saliva, saliva collects on the color development piece of the fixed colour in below after the saliva pad is collected, it finishes to express the sample collection when observing the colour appearance from transparent window, then cover the lower cover to the upper cover position, the post through the inside both sides of lower cover stretches into and holds the chamber extrusion saliva pad, extrude the saliva in the saliva pad, saliva is after the dual filtration of color development piece and fine and close filter paper, flow from holding chamber bottom through-hole and collect in the upper cover, saliva after the filtration is absorbed by the test paper strip, the sample carries out the chromatography on the test paper strip, can judge the result by naked eye through the observation window.
The utility model provides a use method of sample collection purification integral type detection card, when adopting urine or blood sample to examine, put into the container with urine or blood, pull out the upper cover and replace it to the lower cover position, put into the container with the saliva pad down, urine or blood collect on the color development piece after the saliva pad is collected, it finishes to express sample collection when observing from transparent window that the colour appears, then cover the lower cover to the upper cover position, stretch into the chamber of holding through the inside both sides post of lower cover and extrude the saliva pad, urine or blood in the saliva pad, urine or blood flow and collect in the upper cover from holding chamber bottom through-hole after the double filtration of color development piece and fine and close filter paper, urine or blood after the filtration is absorbed by the test paper strip, the sample carries out the chromatography on the test paper strip, can judge the result by naked eye through the observation window.
The sample collection and purification integrated detection card and the use method thereof integrate sample collection and purification, are simple to operate, do not need to be kept in a horizontal state during sample collection, are not limited in operation, have color indication after sample collection is finished, can be taken out from a mouth for prompting, collect and filter the sample from top to bottom, avoid the sample from rushing out of a detection window, and can ensure that sample interference is minimized to the greatest extent and ensure the stability of product performance.
Drawings
Fig. 1 is a schematic structural diagram of a sample collection and purification integrated test card.
Fig. 2 is a schematic sectional view of the sample collection and purification integrated detection card in fig. 1 along the thickness direction after the upper and lower covers are exchanged, and the saliva pad is not shown for clarity.
Fig. 3 is a schematic structural view of another lower cover.
The reference numbers in the figures are: 1-detection card body, 2-upper cover, 3-lower cover, 4-containing cavity, 5-through hole, 6-saliva pad, 7-placing cavity, 8-test paper strip, 9-indicating filter paper, 10-compact filter paper, 11-transparent window, 12-extension part, 13-column, 14-groove, 15-observation window and 16-pressing rod.
Detailed Description
The present invention is further described in detail below with reference to examples so that those skilled in the art can practice the invention with reference to the description.
It will be understood that terms such as "having," "including," and "comprising," when used herein, do not preclude the presence or addition of one or more other elements or groups thereof.
Example 1
As shown in fig. 1-2, a sample collection and purification integrated detection card comprises a detection card body 1, wherein an upper cover 2 is arranged at the upper part of the detection card body, a lower cover 3 is arranged at the lower part of the detection card body, and the upper cover and the lower cover adopt interfaces with the same size, so that the upper cover and the lower cover can be interchanged;
the two sides of the detection card body are respectively provided with an accommodating cavity 4, the accommodating cavities are parallel to the length direction of the detection card body, and the accommodating cavities are opened at the upper end of the detection card body; the bottom of the accommodating cavity is provided with a plurality of through holes 5;
a saliva pad 6 is arranged in the containing cavity, the upper end of the saliva pad extends out of the containing cavity, and the lower end of the saliva pad extends to the bottom of the containing cavity;
the detection card body is provided with a placing cavity 7 with an opening at the lower end of the detection card body in the middle, a test strip 8 is arranged in the placing cavity, the test strip extends downwards out of the placing cavity, and an observation window 15 for observing the detection result of the test strip is processed on the detection card body; in practical application, 1-2 placing cavities can be arranged so as to conveniently place the control test strip to enable the detection result to be more accurate.
The length of the test strip extending out of the placing cavity is not less than 2/3 of the distance between the top of the upper cover and the middle part of the upper end of the detection card body.
And filter paper is arranged between the bottom of the containing cavity and the saliva pad.
The filter paper comprises a color development sheet 9 for primary filtration and displaying saliva absorption conditions and dense filter paper 10 for filtering saliva, wherein the color development sheet is arranged on the dense filter paper. The color development sheet and the dense filter paper carry out double filtration on the saliva so as to better purify the saliva and reduce the detection interference. In this embodiment, the color development sheet is color development filter paper with fixed color by a dry method.
And a transparent window 11 is arranged at the position, corresponding to the color development piece, on the detection card body. The transparent window is connected with the detection card body in a sealing way.
Protruding extension parts 12 are arranged on two sides of the upper end of the detection card body, and the containing cavity is opened at the top ends of the extension parts.
The bottom of the inner side of the lower cover is provided with a pair of columns 13 corresponding to the containing cavity, wherein the columns 13 can extend into the containing cavity to press the saliva pad. In order to better prevent saliva from overflowing from the upper part of the containing cavity when in extrusion, the lower end of the post can be provided with cap-shaped foam or cap-shaped silica gel with certain elastic shape.
A groove 14 is formed in the top of the inner side of the upper cover and corresponds to the test strip, when the upper cover is placed at the position of the lower cover, the lower end of the test strip extends into the groove, and the groove is used for collecting filtered saliva.
Example 2
As shown in fig. 3, similar to the embodiment 1, the difference is that the lower cover is provided with a pressing rod 16 which can slide up and down, the pressing rod penetrates through the bottom of the lower cover to be connected with a pair of columns, and when the saliva pad is squeezed, the pressing rod can drive the columns to enter the accommodating cavity more deeply, so that the squeezing is more sufficient.
Example 3
The utility model provides a use method of sample collection purification integral type detection card, when adopting saliva sample detection, pull out the upper cover and replace it to the lower cover position, hold saliva pad and collect saliva, under gravity and capillary effect, saliva collects on the color development piece of the fixed colour in below after the saliva pad is collected, it finishes to express sample collection when observing from transparent window that there is the colour to appear, then cover the lower cover to the upper cover position, the post through the inside both sides of lower cover stretches into and holds the chamber extrusion saliva pad, extrude the saliva in the saliva pad, saliva is after through color development piece and the double filtration of dense filter paper, flow out from holding the chamber bottom through-hole and collect in the upper cover, the saliva after the filtration is absorbed by the test paper strip, the sample carries out the chromatography on the test paper strip, can judge the result by naked eye through the observation window.
Preferably, the test card is held in an upright position during compression.
Example 4
A method for using a sample collection and purification integrated detection card comprises the steps of placing urine or blood into a container when a urine or blood sample is detected, pulling out an upper cover and replacing the upper cover with the saliva pad, placing a saliva pad downwards into the container, collecting the urine or blood onto a color development sheet after the urine or blood is collected through the saliva pad under the action of capillary effect, indicating that the sample is completely collected when color appears when the color appears is observed from a transparent window, covering the lower cover onto the upper cover, extending into a containing cavity through pillars on two sides inside the lower cover to extrude the saliva pad, extruding the urine or blood out of the saliva pad, filtering the urine or blood through the color development sheet and compact filter paper, flowing out from a through hole at the bottom of the containing cavity and collecting the urine or blood into the upper cover, absorbing the filtered urine or blood by a test paper strip, carrying out chromatography on the test paper strip, and judging a result by naked eyes through an observation window.
While embodiments of the invention have been described above, it is not limited to the applications set forth in the description and the embodiments, which are fully applicable to various fields of endeavor for which the invention may be embodied with additional modifications as would be readily apparent to those skilled in the art, and the invention is therefore not limited to the details given herein and to the embodiments shown and described without departing from the generic concept as defined by the claims and their equivalents.
Claims (10)
1. A sample collection and purification integrated detection card is characterized by comprising a detection card body, wherein an upper cover is arranged at the upper part of the detection card body, a lower cover is arranged at the lower part of the detection card body, and the upper cover and the lower cover adopt interfaces with the same size, so that the upper cover and the lower cover can be interchanged;
accommodating cavities are respectively arranged on two sides of the detection card body, the accommodating cavities are parallel to the length direction of the detection card body, and the accommodating cavities are opened at the upper end of the detection card body; the bottom of the accommodating cavity is provided with a plurality of through holes;
a saliva pad is arranged in the containing cavity, the upper end of the saliva pad extends out of the containing cavity, and the lower end of the saliva pad extends to the bottom of the containing cavity;
the test card comprises a test card body and is characterized in that a placing cavity with an opening at the lower end of the test card body is formed in the middle of the test card body, a test strip is arranged in the placing cavity, the test strip extends out of the placing cavity downwards, and an observation window for observing the test result of the test strip is processed on the test card body;
the length of the test strip extending out of the placing cavity is not less than 2/3 of the distance between the top of the upper cover and the middle part of the upper end of the detection card body.
2. The integrated sample collection and purification test card of claim 1, wherein a filter paper is disposed between the bottom of the containing cavity and the saliva pad.
3. The integrated detection card for sample collection and purification according to claim 2, wherein the filter paper comprises a color development sheet for preliminary filtration and displaying saliva absorption and a dense filter paper for filtering saliva, and the color development sheet is arranged on the dense filter paper.
4. The integrated detection card for sample collection and purification according to claim 3, wherein a transparent window is disposed on the detection card body corresponding to the color development sheet.
5. The integrated sample collection and purification test card of claim 1, wherein the test card body has protruding extensions on both sides of the upper end, and the receiving cavity opens at the top end of the extensions.
6. The integrated test card for sample collection and purification according to claim 5, wherein a pair of posts capable of extending into the containing cavity to press the saliva pad are disposed at the bottom of the inner side of the lower cover corresponding to the containing cavity.
7. The integrated sample collection and purification test card of claim 6, wherein the lower cover is provided with a press rod capable of sliding up and down, and the press rod passes through the bottom of the lower cover and is connected with the pair of pillars.
8. The integrated test card for sample collection and purification according to claim 1, wherein a groove is formed at the top of the inner side of the upper cover corresponding to the test strip, and when the upper cover is placed on the lower cover, the lower end of the test strip extends into the groove.
9. The utility model provides a use method of sample collection purification integral type detection card, a serial communication port, when adopting saliva sample detection, pull out the upper cover and replace it to the lower cover position, hold saliva pad and collect saliva, saliva collects the color development piece of the fixed colour in below after the saliva pad is collected, it finishes to show the sample collection when observing from transparent window that there is the colour to appear, then cover the lower cover to the upper cover position, the post through the inside both sides of lower cover stretches into and holds the chamber extrusion saliva pad, extrude the saliva in the saliva pad, saliva is after the double filtration of color development piece and fine and close filter paper, flow from holding the chamber bottom through-hole and collect in the upper cover, saliva after the filtration is absorbed by the test paper strip, the sample carries out the chromatography on the test paper strip, can judge the result by naked eye through the observation window.
10. The utility model provides a use method of sample collection purification integral type detection card, characterized in that, when adopting urine or blood sample detection, put urine or blood in the container, pull out the upper cover and replace it to the lower cover position, put the saliva pad into the container downwards, urine or blood collect on the color development piece after the saliva pad is collected, it finishes to express sample collection to observe when there is the colour to appear from transparent window, then cover the lower cover to the upper cover position, stretch into the chamber of holding through the post of lower cover inside both sides and extrude the saliva pad, urine or blood in the saliva pad, urine or blood flow from holding the chamber bottom through-hole and collect in the upper cover after two filtration through color development piece and compact filter paper, urine or blood after the filtration are absorbed by the test paper strip, the sample carries out the chromatography on the test paper strip, can judge the result through the observation window by naked eye.
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CN216622178U (en) * | 2021-11-22 | 2022-05-27 | 江苏维尔生物科技有限公司 | Portable detection card for body fluid detection |
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2021
- 2021-11-22 CN CN202111381861.5A patent/CN114113058B/en active Active
Patent Citations (8)
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US20020146346A1 (en) * | 2001-04-04 | 2002-10-10 | Forefront Diagnostics Inc. | "One-device" system for testing constituents in fluids |
CN204422533U (en) * | 2015-02-03 | 2015-06-24 | 常州泰晶生物科技有限公司 | A kind of acquired immune deficiency syndrome (AIDS) urine, saliva rapid detection reagent box |
CN206486520U (en) * | 2016-08-31 | 2017-09-12 | 力因精准医疗产品(上海)有限公司 | A kind of saliva collecting device with filtration |
CN206832818U (en) * | 2017-05-19 | 2018-01-02 | 正元盛邦(天津)生物科技有限公司 | A kind of new ketamine saliva rapid detection reagent box |
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