Disclosure of Invention
The invention aims to provide an SNP molecular marker, a detection primer, a molecular function and an application of a tRNA methyltransferase 10a gene (Trmt 10 a) associated with the growth traits of litopenaeus vannamei, and the SNP molecular marker, the detection primer, the molecular function and the application method of the Trmt10a gene of litopenaeus vannamei, disclosed by the invention, can not be predicted by the conventional knowledge or method at present.
The SNP molecular markers related to the growth traits of the Litopenaeus vannamei tRNA methyltransferase 10a gene (Trmt 10 a) are as follows: the genotype A/A or the genotype A/C at the 6053 th base position of the sequence shown in SEQ ID NO. 1; or a C/C genotype or an A/C genotype at base position 6058; or a T/T genotype or a T/G genotype at the 6534 th base position; or the A/T genotype or the T/T genotype at base position 6865.
Preferably, the A/A genotype at the 6053 th base position, the C/C genotype at the 6058 th base position, the T/T genotype at the 6534 th base position and the A/T genotype at the 6865 th base position of the sequence shown in SEQ ID NO. 1; or the A/C genotype at the 6053 th base position, the A/C genotype at the 6058 th base position, the T/G genotype at the 6534 th base position and the T/T genotype at the 6865 th base position.
Preferably, the A/A genotype individual at the 6053 th base position has a significantly higher growth rate than the A/C genotype individual under the same culture conditions; the growth speed of the C/C genotype at the 6058 th base position under the same culture condition is obviously higher than that of an A/C genotype individual; the growth rate of the T/T genotype individual at the 6534 th base position is obviously higher than that of the T/G genotype individual under the same culture condition; the growth rate of the A/T genotype individual at the 6865 base position under the same breeding condition is obviously higher than that of the T/T genotype individual.
The second objective of the present invention is to provide a detection primer set for the above SNP molecular marker, which comprises the following detection primer pairs:
(1) primer pair for 6053 and 6058 position detection
g.Trmt10a-6053/6058-F:5’-CGGTGTTCATAAAGAGGATAC -3’ ;
g. Trmt10a-6053/6058-R:5’-CTCTCCACTTTTGTGTCGTTC -3’ ;
(2) 6534 position detection primer pair
g. Trmt10a-6534-F:5’-TGTCCAAACAGAATGGCTAC -3’ ;
g. Trmt10a-6534-R:5’-CCTCCCGTGAGCAATACCTT -3’ ;
(3) 6865 th position detection primer pair
g. Trmt10a-6865-F:5’-CAAGGATGAGGAAAATGACTGTG -3’ ;
g. Trmt10a-6865-R:5’- ATTCCAGGTGATTACAAGGTTTACA -3’。
The third purpose of the invention is to provide a detection kit, which comprises the detection primer group of the SNP molecular marker.
The fourth purpose of the invention is to provide the application of the SNP molecular marker associated with the growth traits, the detection primer group of the SNP molecular marker or the detection kit in the breeding of the litopenaeus vannamei.
Preferably, the method is applied to breeding of the rapid growth line of the litopenaeus vannamei.
The fifth purpose of the invention is to provide a method for detecting the growth speed of litopenaeus vannamei, which comprises the following steps: extracting the genomic DNA of the litopenaeus vannamei to be detected, amplifying the set of detection primers of the SNP molecular marker, sequencing to obtain a sequencing result, determining the genotype of the SNP molecular marker based on the sequencing result, and judging the growth speed of the litopenaeus vannamei to be detected.
The method comprises the following steps: the growth speed of the A/A genotype individual at the 6053 th base position is obviously higher than that of the A/C genotype individual under the same culture condition; the growth speed of the C/C genotype at the 6058 th base position under the same culture condition is obviously higher than that of an A/C genotype individual; the growth rate of the T/T genotype individual at the 6534 th base position is obviously higher than that of the T/G genotype individual under the same culture condition; the growth rate of the A/T genotype individual at the 6865 base position under the same breeding condition is obviously higher than that of the T/T genotype individual.
The rapid growth is an important economic character of the Litopenaeus vannamei, and in the fine breed breeding work of the Litopenaeus vannamei, the SNP molecular marker and the method can be used for screening a backup parent group, so that a Litopenaeus vannamei parent individual with slow growth can be eliminated as soon as possible, and the growth speed of the offspring of the Litopenaeus vannamei can be enhanced rapidly. The method provided by the invention is beneficial to rapidly increasing the growth speed of the offspring of the litopenaeus vannamei, shortening the breeding cycle of excellent prawn strains and having a great potential application value.
The specific implementation mode is as follows:
the following examples are further illustrative of the present invention and are not intended to be limiting thereof.
Example 1:
the Litopenaeus vannamei colony used for analysis comprises 1 holosib family and 2 hemisib families, after 1.5 months of culture under the same condition, 215 individuals are randomly selected for weight measurement, and the genomic DNA of the Litopenaeus vannamei is extracted according to a Tiangen marine animal tissue genome extraction kit (TIANGEN, Tiangen Biochemical technology, Ltd.).
The PCR detection is carried out on the Trmt10a gene of the 215-tailed prawn individual by using the detection primer (F: 5'-TCAATGGTGTTCATAAAGAGGA-3', R: 5'-CCTAAGCTAATCAGTATCAG-3'), and the reaction program is as follows: pre-denaturation at 95 ℃ for 4 min, (denaturation at 95 ℃ for 30 sec, annealing at 52-57 ℃ for 20 sec, extension at 72 ℃ for 1 min) x 35 cycles, and final extension at 72 ℃ for 10 min. Sequencing the PCR product, wherein the sequence of the Trmt10a gene is shown as SEQ ID NO.1, and specifically comprises the following steps:
cagacttacgcgaacgccatgttaacacgtgaaaaagccaatatgttgcgagaatttacaattatctttaccttatgtgccttttaatgcctgttcgtgtctaggagtgctcgtagataagaagaaagggggagaaaaggtgagattgaacgagagtttgtgaatgataaggtaatgtgttgtatttttttgacacgagaggaaaaaagactgtatattgtctataagatttaggttaggttaggttaggttaggttagttaggttaggttagttaggttaggttaggttataggttaggttaggttaggttaggttaggttaggttaggttataggttaggttaggttaggttataggttaggttaggttaggttataggttaggttaggttaggttaggttaggttaggtttaggttaggttaggttaggttaggttaggttaggttaggttaggttaggttaggtttaggttagggttagggttaggttaggttaggttataggttaggttaggttaggttaggttataggttaggttaggttaggtttaggttaggttaggttaggttaggttaggttaggttaggttataggttaggttaggttaaggttaggttaggttaggttaggttaggttaggttaggttaggttaggttaggttaggttaggtttaggttagggttaggttaggttaggttaggttaggttataggtttaggttaggttaggttataggttaggttaggttggttaggttaggttaggttaggttaggttaggttaggttataggttaggttaggttaggttaggttaggttaggttaggttaggtttaggttaggtttataggtttaggtttaggttaggttaggttaggttaggttaggttaggttaggttaggttaggttaggttaggttaggttaggttaggttatagggttaggttagttaggttataggttagttaggttataggttaggttaggttataggttaggttatagtttaggttaggttataggttataggttaggttaggttaggttaggttaggttaggttagggttaggttagggttaggtttaggttaggttagggttaggttatagttaggttaggtttaggttaggttaggttaggttggttaggttaggttaggttaggtaggttaggttaggttaggttaggttaggttttaggttaggttaggttagggttaggttagttaggttaggttaggttaggttaggttataggtttaggttaggttaggttaggttaggttaggttatagtttaggttaggttaggttaggttaggttaggttaggttaggttaggttaggttaggttaggttaggttaggttaggttaggttagggttaggttaggttatagtttaggtaggttagggttagggttggttaggttataggttaggttaggttaggtttaggttaggttaggttaggttattaggttaggttaggttaggttaggttaggttaggttaggttaggttaggttaggttataggttaggttaggttataggttaggttaggttaggttagttaggttaggttaggttaggttaggttataggttaggttagttaggttaggtttaggttaggttaggttaggttaggttaggttaggttatagggttaggttaggttaggttaggtttaggttaggttggttaggttagggttaggttaggttaggtttttaggttaggttaggttaggttagggttataggttaggttaggttagggttaggttaggttagggttaggttaagttaggttaggttaggttaggtaggttataggttaggttttaggttaggttataggttaggttaggtttaggttaggttaggtaggttaggttagtttagttttaggttatagttagtaggttaggttgttaggtttaggttaggttatagttaggttaggttaggttggtttaggttaaggttaggttatagggttaggttaggttaggttatagggttaggttaggataggtttataggttagcttataggtttataggtttaggttaaggttagttagttagggttaggttaggttgggggttataggttaggttataggttataggttataagttgggttaggttaggttaggttaagttatggttttaggttaggttaggttatatggttgggttaggttaggttataggttaggttaggttatattaggttatagttgggttaggttataaggttaggttaggttgggttgggttatagttaggttaggttataggttgggttaggtaggttagaggttatgttaggttaggttaggttagtaggttaggttagaggttatgttaggttaggttataggttaagttaggttaggttaggttatagttaggtttaggttataggttaggttaggttaggttattttggttaggttaggttgtaggttaggttaggttatagtctaggttaggttataaggttaggttatagttgggttaggttaggttaggttaggttaggttaggttaggttaggttaggttaggtttaggttagttatctaggttaggttatatatatatatatatatatatatataatatatatatatatatatatatatatatatatatatagttaggttaggttaggttaacctataacctaacttaacctaatctataacctaacctaactaactataacctaaacctaacctataacctaacctacctaaacctactaacctatgctaacttaacctaatctataacctaacctaacctaacctataacctaaaccctaactaacctaacctaacctaaacctaacctacctaaactaacctaactaacctaacttaacctaatctataacctaacctaacctaacctattaacctaacctaaccttaactataaccaacccaacctaacctaacctatgacctaacctaacctataacctaacctaacccttaacctaactaactaacctataacctaactaccaaaaatataacctactaacctatacctaacctaacctaacctataacctaacctaacctaacctaaagcctataacctaacctaacctaactatacctaacctaacctaacctaactaacctaacctataacctaacctaacccctaacctaaccctaacctaataacctataacctaaccctataactaacctaacctatacctatataacctaacctatcctaacctataacctaactaactaacttttaacctaacctaatctataaactaacctaaacctaacctaacctataactaactaacctacctaacctaacctaacctaacctatataaccctaaaccctaacctaacctacctataacctaacctaactaacctataacctaacctaacctaaactaaaataacctaacctataacctaacctaacctataacctaacctaacctaaaacctaacctaacctaacctaacctaacctaacctaaccctaacctaacctataaccaacctaaaccctaacctatacctaacctaacctaaacctaacctaaccctacctaacctataacctaacctaacctacctaacctataacctaacctaacctaacctaacctaacctaacctataacctaacctaacctaacctaaacctaactaaaacctaacctaacctaacctaacctaaactataacctaacctaacctaacctataacctataacctaacctaacctaacctaacctaacctaacctaactataacctaacctaacctaaacctaaccctaacctaacctaactaaacctaacctaaaacctaaaacctataacctaacctaacctaacctataacctaacctaacctataacctaacctaacctaactataacctaacctaacctaaccctaacctataacctaacctaacctaactataactaactaacctaacctaaccctaactaacctaacctataacctaacctaacctaacctaacctataacctaacctaacctaacctataacctaaacctaacctataacctaacctaacctaactaacctataacctaacctaacctaaacctaacctaacctataacctaacctaacctaacctaacctatacctaacctaacctaacctaactaacctaacctaacctaacctaacctaactaacctataacctaacctaacctacctaacctaacctaacctaaacctaacctataacctaacctaacctaaacctaacctaaacctataaccttaacctaaacctaacctaacctaacaaacctataaacctaacctaacctataaaacctaacctaacctaacctataacctaactaacctaacctataacctaacctaacctaacctaaacctaacctatacctaacctaacctaaacctaacctaacctaacctataacctaacctaacctaacctaacctaacctataacctaacctataacctaacctataacctaacctaacctaacctaacctaacctaacctacctaacctaacctataacctaacctaaacctatacctaacctaacctaacctataacctaacctaacctaacctaacctaacctaaacctaacctacctataacctaacctaactataacctaacctaacctaactaacctaacctaacctaacctaacctataacctaacctaacctaacctataacctaactaacctaacctaacctaacctaacctataacctataacctaacctaacctaacctataacctaacctataacctaacctaacctaacctaacctataacctaacctataacctaacctataacctaacctaacctaacctaacctaaaacctacctataacctaacctaacctaacctaacctaacctacctaaccctaacctaacctaactataacctaacctaacctaacctatacctaacctaacctaaaacctaacctaacctaacctaaacctaaaccctatacctaacctaacctaacctataacctaacctaacctaacctaacctataacctaacctaacctaacctaacctataacctaacctaacctaacctaacctaacctaacctaacctaacctaacctaacctaacctaacctaacctataacctaacctaacctaatactgttatgataacagtaattataatgaaacatattgatattaataatgataatgatatttgtgtgatgataatgataatgataatgtcaatagtaataataatgatgtgaactagaatttacattccaaattctaaattctgagtttagcttgttcatagttatttataaataactattccagtttgtctatatttatcttgtaagcagtatacagttatgtatattatgtgtaaggtaattgataacaagtgtgatattttgagtagcagctcaaattgtgatattgacttattgataacaagacaccctttatatatgcacaatccactgtgttttgtgttattttgtgcaaagatggatgaagcacgtgaagccatccatctataaacaaaattaaacaaagcttcagtggatgatgtatgcaatcaatgccagtaagttaatatttgagaacttgataaggaagacatttaaacttttttttctacttattgtattgttttgcatgcttctctattgtaatatttgataatgagggcatttaaagtttctttttttttctatttattgtgctattgttgcatgcttctctattgtaagtgtttgaatgcattatttcagaaagcatttcttgtgtttcaggttaattcgccagcacaatggagtcgacagaggttatcaacggtgttcataaagaggatacacttctcgacaacagacctccctcagttgaagaaaacagcaacatgaacaacaacgacgacaacaagccttcacccgatccggagcagagtgccaatggaggagaaactcagacgttatccaaaagacagcagaagaagcttttgagaagacagaagtggaacgacacaaaagtggagagaagaaagagggagcgggagaagctgaaaaagagattggtggagaagagggcggccggagagccctgcggcaacatcaggaagaagttgaagcagatgaccatggcgagtagttcgtgtaaacagcgggttgttgtagacatgtcctttgatgacctcatgattgagaagcatttgtctcagtgcgttaaacaagttagccgttgttatagtgccaaccggagagtcagtagccctatgcaactctatgttacaagctttgaggggaaatgtgcagaggtcatgtccaaacagaatggctacgaacactgggatgtgtactttaaaaaggagaattatatggatcttttccccaaagaagacataatatacctcacaagtgaatcaaggaatgtgattacgtcagtggatgaaaacaaagtgtatgtcattggaggcttggtggaccacaatgtgcataaagggttgtgtctaagattggctgaagagaaaggtattgctcacgggaggcttccaatagacgaattcattgagatgaagacgagaaaagtcttgaccatcgatcacgtgttccgcattatactaggagtcacggaagggggttcgtggaaggaatctttcctgaagactatcccggcaaggaagggggcagtcggcaaggatgaggaaaatgactgtgaagattccgaattggaaaaggatgcgactaacaattcagacgacaagagcgaagacaacccttcagagagcagagagcctgtatgcgaaggtgatcccgagaagagctgatactgattagcttaggctgtttattgttactggaattatattttatgcttcttctctttgttgtttatattctcttttttaattaaattcacatcaggtttatatgtaaaccttgtaatcacctggaatttatgacttatcagattgatgagaatattaaatgggtgtttgtgagctgtgacaaaaatcccatgcgttgggtatttttattatatgtaattttagctcaaagcaaagtaagaagaaaatttgaagaaagtttatcacttttagtccaagcacacataggcctatatgccatgataaaactgtatcagaaatccaataattatataaaggattaaagaat。
the cDNA sequence is shown in SEQ ID NO.2, and through statistics of individual genotypes, the cDNA sequence shows that:
(1) the growth speed of the A/A genotype individual at the 6053 th base position is obviously higher than that of the A/C genotype individual under the same culture condition; the growth speed of the C/C genotype at the 6058 th base position under the same culture condition is obviously higher than that of an A/C genotype individual; the growth rate of the T/T genotype individual at the 6534 th base position is obviously higher than that of the T/G genotype individual under the same culture condition; the growth rate of the A/T genotype individual at the 6865 base position is obviously higher than that of the T/T genotype individual under the same culture condition (figure 2, table 1);
(2) missense mutation at 6053 base position C > A results in mutation of the amino acid encoded by the gene from threonine (Thr, T) to asparagine (Asn, N); missense mutation at base 6058 position A > C results in mutation of the amino acid encoded by the gene from threonine (Thr, T) to proline (Pro, P); the 6534 base position T > G missense mutation causes the mutation of the coding amino acid of the gene from aspartic acid (Asp, D) to glutamic acid (Glu, E); the missense mutation at the 6865 th base position T > A causes the mutation of the coded amino acid of the gene from serine (Ser, S) to threonine (Thr, T) (A in figure 1);
TABLE 1 correlation analysis of SNP sites and growth traits of the Trmt10a gene of prawn
Sequence listing
<110> animal science institute of academy of agricultural sciences of Guangdong province
<120> snp molecular marker associated with growth traits of litopenaeus vannamei, detection primer and application thereof
<160> 2
<170> SIPOSequenceListing 1.0
<210> 1
<211> 7261
<212> DNA
<213> Litopenaeus vannamei (Litopenaeus vannamei)
<400> 1
cagacttacg cgaacgccat gttaacacgt gaaaaagcca atatgttgcg agaatttaca 60
attatcttta ccttatgtgc cttttaatgc ctgttcgtgt ctaggagtgc tcgtagataa 120
gaagaaaggg ggagaaaagg tgagattgaa cgagagtttg tgaatgataa ggtaatgtgt 180
tgtatttttt tgacacgaga ggaaaaaaga ctgtatattg tctataagat ttaggttagg 240
ttaggttagg ttaggttagt taggttaggt tagttaggtt aggttaggtt ataggttagg 300
ttaggttagg ttaggttagg ttaggttagg ttataggtta ggttaggtta ggttataggt 360
taggttaggt taggttatag gttaggttag gttaggttag gttaggttag gtttaggtta 420
ggttaggtta ggttaggtta ggttaggtta ggttaggtta ggttaggttt aggttagggt 480
tagggttagg ttaggttagg ttataggtta ggttaggtta ggttaggtta taggttaggt 540
taggttaggt ttaggttagg ttaggttagg ttaggttagg ttaggttagg ttataggtta 600
ggttaggtta aggttaggtt aggttaggtt aggttaggtt aggttaggtt aggttaggtt 660
aggttaggtt aggtttaggt tagggttagg ttaggttagg ttaggttagg ttataggttt 720
aggttaggtt aggttatagg ttaggttagg ttggttaggt taggttaggt taggttaggt 780
taggttaggt tataggttag gttaggttag gttaggttag gttaggttag gttaggttta 840
ggttaggttt ataggtttag gtttaggtta ggttaggtta ggttaggtta ggttaggtta 900
ggttaggtta ggttaggtta ggttaggtta ggttaggtta tagggttagg ttagttaggt 960
tataggttag ttaggttata ggttaggtta ggttataggt taggttatag tttaggttag 1020
gttataggtt ataggttagg ttaggttagg ttaggttagg ttaggttagg gttaggttag 1080
ggttaggttt aggttaggtt agggttaggt tatagttagg ttaggtttag gttaggttag 1140
gttaggttgg ttaggttagg ttaggttagg taggttaggt taggttaggt taggttaggt 1200
tttaggttag gttaggttag ggttaggtta gttaggttag gttaggttag gttaggttat 1260
aggtttaggt taggttaggt taggttaggt taggttatag tttaggttag gttaggttag 1320
gttaggttag gttaggttag gttaggttag gttaggttag gttaggttag gttaggttag 1380
gttagggtta ggttaggtta tagtttaggt aggttagggt tagggttggt taggttatag 1440
gttaggttag gttaggttta ggttaggtta ggttaggtta ttaggttagg ttaggttagg 1500
ttaggttagg ttaggttagg ttaggttagg ttaggttata ggttaggtta ggttataggt 1560
taggttaggt taggttagtt aggttaggtt aggttaggtt aggttatagg ttaggttagt 1620
taggttaggt ttaggttagg ttaggttagg ttaggttagg ttaggttata gggttaggtt 1680
aggttaggtt aggtttaggt taggttggtt aggttagggt taggttaggt taggttttta 1740
ggttaggtta ggttaggtta gggttatagg ttaggttagg ttagggttag gttaggttag 1800
ggttaggtta agttaggtta ggttaggtta ggtaggttat aggttaggtt ttaggttagg 1860
ttataggtta ggttaggttt aggttaggtt aggtaggtta ggttagttta gttttaggtt 1920
atagttagta ggttaggttg ttaggtttag gttaggttat agttaggtta ggttaggttg 1980
gtttaggtta aggttaggtt atagggttag gttaggttag gttatagggt taggttagga 2040
taggtttata ggttagctta taggtttata ggtttaggtt aaggttagtt agttagggtt 2100
aggttaggtt gggggttata ggttaggtta taggttatag gttataagtt gggttaggtt 2160
aggttaggtt aagttatggt tttaggttag gttaggttat atggttgggt taggttaggt 2220
tataggttag gttaggttat attaggttat agttgggtta ggttataagg ttaggttagg 2280
ttgggttggg ttatagttag gttaggttat aggttgggtt aggtaggtta gaggttatgt 2340
taggttaggt taggttagta ggttaggtta gaggttatgt taggttaggt tataggttaa 2400
gttaggttag gttaggttat agttaggttt aggttatagg ttaggttagg ttaggttatt 2460
ttggttaggt taggttgtag gttaggttag gttatagtct aggttaggtt ataaggttag 2520
gttatagttg ggttaggtta ggttaggtta ggttaggtta ggttaggtta ggttaggtta 2580
ggtttaggtt agttatctag gttaggttat atatatatat atatatatat atataatata 2640
tatatatata tatatatata tatatatata tagttaggtt aggttaggtt aacctataac 2700
ctaacttaac ctaatctata acctaaccta actaactata acctaaacct aacctataac 2760
ctaacctacc taaacctact aacctatgct aacttaacct aatctataac ctaacctaac 2820
ctaacctata acctaaaccc taactaacct aacctaacct aaacctaacc tacctaaact 2880
aacctaacta acctaactta acctaatcta taacctaacc taacctaacc tattaaccta 2940
acctaacctt aactataacc aacccaacct aacctaacct atgacctaac ctaacctata 3000
acctaaccta acccttaacc taactaacta acctataacc taactaccaa aaatataacc 3060
tactaaccta tacctaacct aacctaacct ataacctaac ctaacctaac ctaaagccta 3120
taacctaacc taacctaact atacctaacc taacctaacc taactaacct aacctataac 3180
ctaacctaac ccctaaccta accctaacct aataacctat aacctaaccc tataactaac 3240
ctaacctata cctatataac ctaacctatc ctaacctata acctaactaa ctaactttta 3300
acctaaccta atctataaac taacctaaac ctaacctaac ctataactaa ctaacctacc 3360
taacctaacc taacctaacc tatataaccc taaaccctaa cctaacctac ctataaccta 3420
acctaactaa cctataacct aacctaacct aaactaaaat aacctaacct ataacctaac 3480
ctaacctata acctaaccta acctaaaacc taacctaacc taacctaacc taacctaacc 3540
taaccctaac ctaacctata accaacctaa accctaacct atacctaacc taacctaaac 3600
ctaacctaac cctacctaac ctataaccta acctaaccta cctaacctat aacctaacct 3660
aacctaacct aacctaacct aacctataac ctaacctaac ctaacctaaa cctaactaaa 3720
acctaaccta acctaaccta acctaaacta taacctaacc taacctaacc tataacctat 3780
aacctaacct aacctaacct aacctaacct aacctaacta taacctaacc taacctaaac 3840
ctaaccctaa cctaacctaa ctaaacctaa cctaaaacct aaaacctata acctaaccta 3900
acctaaccta taacctaacc taacctataa cctaacctaa cctaactata acctaaccta 3960
acctaaccct aacctataac ctaacctaac ctaactataa ctaactaacc taacctaacc 4020
ctaactaacc taacctataa cctaacctaa cctaacctaa cctataacct aacctaacct 4080
aacctataac ctaaacctaa cctataacct aacctaacct aactaaccta taacctaacc 4140
taacctaaac ctaacctaac ctataaccta acctaaccta acctaaccta tacctaacct 4200
aacctaacct aactaaccta acctaaccta acctaaccta actaacctat aacctaacct 4260
aacctaccta acctaaccta acctaaacct aacctataac ctaacctaac ctaaacctaa 4320
cctaaaccta taaccttaac ctaaacctaa cctaacctaa caaacctata aacctaacct 4380
aacctataaa acctaaccta acctaaccta taacctaact aacctaacct ataacctaac 4440
ctaacctaac ctaaacctaa cctataccta acctaaccta aacctaacct aacctaacct 4500
ataacctaac ctaacctaac ctaacctaac ctataaccta acctataacc taacctataa 4560
cctaacctaa cctaacctaa cctaacctaa cctacctaac ctaacctata acctaaccta 4620
aacctatacc taacctaacc taacctataa cctaacctaa cctaacctaa cctaacctaa 4680
acctaaccta cctataacct aacctaacta taacctaacc taacctaact aacctaacct 4740
aacctaacct aacctataac ctaacctaac ctaacctata acctaactaa cctaacctaa 4800
cctaacctaa cctataacct ataacctaac ctaacctaac ctataaccta acctataacc 4860
taacctaacc taacctaacc tataacctaa cctataacct aacctataac ctaacctaac 4920
ctaacctaac ctaaaaccta cctataacct aacctaacct aacctaacct aacctaccta 4980
accctaacct aacctaacta taacctaacc taacctaacc tatacctaac ctaacctaaa 5040
acctaaccta acctaaccta aacctaaacc ctatacctaa cctaacctaa cctataacct 5100
aacctaacct aacctaacct ataacctaac ctaacctaac ctaacctata acctaaccta 5160
acctaaccta acctaaccta acctaaccta acctaaccta acctaaccta acctaaccta 5220
taacctaacc taacctaata ctgttatgat aacagtaatt ataatgaaac atattgatat 5280
taataatgat aatgatattt gtgtgatgat aatgataatg ataatgtcaa tagtaataat 5340
aatgatgtga actagaattt acattccaaa ttctaaattc tgagtttagc ttgttcatag 5400
ttatttataa ataactattc cagtttgtct atatttatct tgtaagcagt atacagttat 5460
gtatattatg tgtaaggtaa ttgataacaa gtgtgatatt ttgagtagca gctcaaattg 5520
tgatattgac ttattgataa caagacaccc tttatatatg cacaatccac tgtgttttgt 5580
gttattttgt gcaaagatgg atgaagcacg tgaagccatc catctataaa caaaattaaa 5640
caaagcttca gtggatgatg tatgcaatca atgccagtaa gttaatattt gagaacttga 5700
taaggaagac atttaaactt ttttttctac ttattgtatt gttttgcatg cttctctatt 5760
gtaatatttg ataatgaggg catttaaagt ttcttttttt ttctatttat tgtgctattg 5820
ttgcatgctt ctctattgta agtgtttgaa tgcattattt cagaaagcat ttcttgtgtt 5880
tcaggttaat tcgccagcac aatggagtcg acagaggtta tcaacggtgt tcataaagag 5940
gatacacttc tcgacaacag acctccctca gttgaagaaa acagcaacat gaacaacaac 6000
gacgacaaca agccttcacc cgatccggag cagagtgcca atggaggaga aactcagacg 6060
ttatccaaaa gacagcagaa gaagcttttg agaagacaga agtggaacga cacaaaagtg 6120
gagagaagaa agagggagcg ggagaagctg aaaaagagat tggtggagaa gagggcggcc 6180
ggagagccct gcggcaacat caggaagaag ttgaagcaga tgaccatggc gagtagttcg 6240
tgtaaacagc gggttgttgt agacatgtcc tttgatgacc tcatgattga gaagcatttg 6300
tctcagtgcg ttaaacaagt tagccgttgt tatagtgcca accggagagt cagtagccct 6360
atgcaactct atgttacaag ctttgagggg aaatgtgcag aggtcatgtc caaacagaat 6420
ggctacgaac actgggatgt gtactttaaa aaggagaatt atatggatct tttccccaaa 6480
gaagacataa tatacctcac aagtgaatca aggaatgtga ttacgtcagt ggatgaaaac 6540
aaagtgtatg tcattggagg cttggtggac cacaatgtgc ataaagggtt gtgtctaaga 6600
ttggctgaag agaaaggtat tgctcacggg aggcttccaa tagacgaatt cattgagatg 6660
aagacgagaa aagtcttgac catcgatcac gtgttccgca ttatactagg agtcacggaa 6720
gggggttcgt ggaaggaatc tttcctgaag actatcccgg caaggaaggg ggcagtcggc 6780
aaggatgagg aaaatgactg tgaagattcc gaattggaaa aggatgcgac taacaattca 6840
gacgacaaga gcgaagacaa cccttcagag agcagagagc ctgtatgcga aggtgatccc 6900
gagaagagct gatactgatt agcttaggct gtttattgtt actggaatta tattttatgc 6960
ttcttctctt tgttgtttat attctctttt ttaattaaat tcacatcagg tttatatgta 7020
aaccttgtaa tcacctggaa tttatgactt atcagattga tgagaatatt aaatgggtgt 7080
ttgtgagctg tgacaaaaat cccatgcgtt gggtattttt attatatgta attttagctc 7140
aaagcaaagt aagaagaaaa tttgaagaaa gtttatcact tttagtccaa gcacacatag 7200
gcctatatgc catgataaaa ctgtatcaga aatccaataa ttatataaag gattaaagaa 7260
t 7261
<210> 2
<211> 1516
<212> DNA
<213> Litopenaeus vannamei (Litopenaeus vannamei)
<400> 2
cagacttacg cgaacgccat gttaacacgt gaaaaagcca atatgttgcg agaatttaca 60
attatcttta ccttatgtgc cttttaatgc ctgttcgtgt ctaggagtgc tcgtagataa 120
gaagaaaggg ggagaaaagg ttaattcgcc agcacaatgg agtcgacaga ggttatcaac 180
ggtgttcata aagaggatac acttctcgac aacagacctc cctcagttga agaaaacagc 240
aacatgaaca acaacgacga caacaagcct tcacccgatc cggagcagag tgccaatgga 300
ggagaaactc agccgttatc caaaagacag cagaagaagc ttttgagaag acagaagtgg 360
aacgacacaa aagtggagag aagaaagagg gagcgggaga agctgaaaaa gagattggtg 420
gagaagaggg cggccggaga gccctgcggc aacatcagga agaagttgaa gcagatgacc 480
atggcgagta gttcgtgtaa acagcgggtt gttgtagaca tgtcctttga tgacctcatg 540
attgagaagc atttgtctca gtgcgttaaa caagttagcc gttgttatag tgccaaccgg 600
agagtcagta gccctatgca actctatgtt acaagctttg aggggaaatg tgcagaggtc 660
atgtccaaac agaatggcta cgaacactgg gatgtgtact ttaaaaagga gaattatatg 720
gatcttttcc ccaaagaaga cataatatac ctcacaagtg aatcaaggaa tgtgattacg 780
tcagtggatg aaaacaaagt gtatgtcatt ggaggcttgg tggaccacaa tgtgcataaa 840
gggttgtgtc taagattggc tgaagagaaa ggtattgctc acgggaggct tccaatagac 900
gaattcattg agatgaagac gagaaaagtc ttgaccatcg atcacgtgtt ccgcattata 960
ctaggagtca cggaaggggg ttcgtggaag gaatctttcc tgaagactat cccggcaagg 1020
aagggggcag tcggcaagga tgaggaaaat gactgtgaag attccgaatt ggaaaaggat 1080
gcgactaaca attcagacga caagagcgaa gacaaccctt cagagagcag agagcctgta 1140
tgcgaaggtg atcccgagaa gagctgatac tgattagctt aggctgttta ttgttactgg 1200
aattatattt tatgcttctt ctctttgttg tttatattct cttttttaat taaattcaca 1260
tcaggtttat atgtaaacct tgtaatcacc tggaatttat gacttatcag attgatgaga 1320
atattaaatg ggtgtttgtg agctgtgaca aaaatcccat gcgttgggta tttttattat 1380
atgtaatttt agctcaaagc aaagtaagaa gaaaatttga agaaagttta tcacttttag 1440
tccaagcaca cataggccta tatgccatga taaaactgta tcagaaatcc aataattata 1500
taaaggatta aagaat 1516