CN114107166A - Cell culture method and application thereof - Google Patents

Cell culture method and application thereof Download PDF

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Publication number
CN114107166A
CN114107166A CN202111432252.8A CN202111432252A CN114107166A CN 114107166 A CN114107166 A CN 114107166A CN 202111432252 A CN202111432252 A CN 202111432252A CN 114107166 A CN114107166 A CN 114107166A
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supporting
culture
cell culture
illumination
lamp
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CN202111432252.8A
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Chinese (zh)
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赵倩
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04—Plant cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/10—Petri dish
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/46—Means for fastening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/50—Means for positioning or orientating the apparatus

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
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  • Cell Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to the field of cell culture, in particular to a cell culture method and application thereof, which are convenient for periodically illuminating cultured cells to influence the culture of plant cells; the method comprises the following steps: s1, taking the explant for disinfection, and then directly separating cells from the explant; s2, placing the separated cells in a culture dish filled with a culture medium; s3, placing the culture dish on a supporting device for illumination culture; s4, controlling the culture temperature and appropriately supplementing a fresh culture solution; the supporting device comprises a base, a supporting plate, a supporting arm shaft, a supporting mechanism, a rotary table and an illumination mechanism, wherein the supporting plate is fixed on the base, the supporting arm shaft is transversely fixed at the upper end of the supporting plate, the supporting mechanism for placing a culture dish is connected to one end of the supporting arm shaft, the rotary table rotates on the supporting arm shaft, and the illumination mechanism is connected to the rotary table; the cell culture method is applied to the differentiation culture of the vegetative cells.

Description

Cell culture method and application thereof
Technical Field
The invention relates to the field of cell culture, in particular to a cell culture method and application thereof.
Background
The cell culture comprises animal cell culture and plant cell culture, wherein the animal cell culture refers to a method for simulating basic conditions and environments in a normal physiological state in vivo under the in vitro aseptic condition of an organism, separating and culturing organism tissue cells or establishing a cell line, and enabling the cells to grow or propagate in an in vitro culture container for a long time; the plant cell culture is a technology of placing callus or other easily-dispersible tissues in a liquid culture medium under an in vitro condition for shake culture to obtain dispersed free suspension cells, and proliferating the cells through subculture to obtain a large number of cell populations.
In the process of culturing plant cells, illumination is closely related to cell differentiation, but the conventional culture equipment is inconvenient for periodically illuminating the cultured cells to influence the culture of the plant cells.
Disclosure of Invention
The invention aims to provide a cell culture method and application thereof, which are convenient for periodically illuminating cultured cells to influence the culture of plant cells.
A cell culture method comprising the steps of:
s1, taking the explant for disinfection, and then directly separating cells from the explant;
s2, placing the separated cells in a culture dish filled with a culture medium;
s3, placing the culture dish on a supporting device for illumination culture;
s4, controlling the culture temperature and appropriately supplementing fresh culture solution.
The supporting device comprises a base, a supporting plate, a supporting arm shaft, a supporting mechanism, a rotary table and an illumination mechanism, wherein the supporting plate is fixed on the base, the supporting arm shaft is transversely fixed at the upper end of the supporting plate, the supporting mechanism used for placing a culture dish is connected to one end of the supporting arm shaft, the rotary table rotates on the supporting arm shaft, and the illumination mechanism is connected to the rotary table.
The illumination mechanism comprises a lamp holder, an illumination lamp and a magnetic plate, the lamp holder is connected to the rotary plate, the magnetic plate is arranged at the lower end of the lamp holder, and the illumination lamp is rotatably connected to the lamp holder.
The illumination mechanism further comprises an adjusting screw rod, the rotating disc is rotated with the adjusting screw rod, and the lamp holder is in threaded connection with the adjusting screw rod.
The illumination mechanism further comprises a lamp tube clamp, the lamp tube clamp is fixed on the illumination lamp, and the adjusting screw rod is in sliding connection with the lamp tube clamp in a matched mode.
The lifting mechanism comprises a supporting ring, a rotating ring, mounting long holes and a bracket, wherein the supporting ring is fixed on a supporting arm shaft, the rotating ring rotates in the supporting ring, the lower end of the rotating ring is uniformly provided with three mounting long holes, and the bracket slides in the three mounting long holes.
The bracket comprises a sliding seat, a square rod, a lifting spring, a supporting plate and a limiting column, wherein the supporting plate is fixed at the upper end of the square rod, the limiting column is fixed at the lower end of the square rod, the square rod is provided with the sliding seat in a sliding manner, the lifting spring is arranged between the sliding seat and the supporting plate, and the sliding seat is connected with the installation long hole in a sliding manner.
The supporting and placing mechanism further comprises a transmission disc and a clamping screw rod, the transmission disc is arranged at the lower end of the rotating ring in a rotating mode, the transmission disc is connected with the three sliding seats in a sliding mode, the clamping screw rod is arranged on one of the sliding seats in a rotating mode, and the clamping screw rod is in threaded connection with the rotating ring.
The supporting mechanism further comprises two clamping columns, and the two clamping columns are symmetrically fixed on the supporting plate far away from the center end of the rotating ring.
The cell culture method is applied to the differentiation culture of the vegetative cells.
Drawings
FIG. 1 is a flow diagram of a cell culture method;
FIG. 2 is a schematic structural view of the support device;
FIG. 3 is a partial schematic structural view of the support device;
FIG. 4 is a schematic structural view of the turntable;
FIG. 5 is a schematic view of the swivel;
FIG. 6 is a schematic view of an embodiment of a tray holding a culture dish;
FIG. 7 is a schematic structural diagram of a racking mechanism;
FIG. 8 is a schematic view of the clamping position limiting plate;
FIG. 9 is a schematic structural view of a vibrating ring frame;
FIG. 10 is a schematic view of the transmission frame;
FIG. 11 is a first schematic structural view of an illumination mechanism;
fig. 12 is a schematic structural diagram of the illumination mechanism.
In the figure:
a base 101; a support plate 102; an arm shaft 103; a ring 104; a turntable 105; a support square bar 106; a crossbar frame 107;
a swivel 201; a drive plate 202; the mounting long hole 203; a link shaft 204; a clamping screw 205;
a slide mount 301; a square bar 302; a lift spring 303; a pallet 304; a clamp post 305; a spacing post 306;
clamping the limit plate 401; a clamping spring 402; a clamping bar 403;
a triangular prism plate 501; a vibrating ring frame 502; rounded teeth 503; a threaded sleeve 504;
a transmission frame 601; a drive wheel 602; a couple wheel 603;
an adjusting screw 701; a lamp socket 702; an illumination lamp 703; a lamp clamp 704; a magnetic plate 705.
Detailed Description
As shown in fig. 1:
a cell culture method comprising the steps of:
s1, taking the explant for disinfection, and then directly separating cells from the explant;
s2, placing the separated cells in a culture dish filled with a culture medium;
s3, placing the culture dish on a supporting device for illumination culture;
s4, controlling the culture temperature and appropriately supplementing fresh culture solution.
As shown in fig. 2-12:
the supporting device comprises a base 101, a support plate 102, a support arm shaft 103, a supporting mechanism, a turntable 105 and an illumination mechanism, wherein the support plate 102 is fixed on the base 101, the support arm shaft 103 is transversely fixed at the upper end of the support plate 102, the supporting mechanism for placing a culture dish is connected to one end of the support arm shaft 103, the turntable 105 rotates on the support arm shaft 103, and the illumination mechanism is connected to the turntable 105.
In use, the base 101 is used to support the device, placing the culture dish horizontally when it is placed on the racking mechanism; through installing the reciprocating motor on board 102, carry out reciprocating motion to carousel 105 to drive illumination mechanism and use boom shaft 103 to rotate as the axle is reciprocal, thereby form and carry out periodic reciprocal illumination to the culture dish on the support mechanism, and can adjust the cycle of illumination angle through control carousel 105 slew velocity, further form the illumination cultivation to explant cell.
As shown in fig. 2-12:
the illumination mechanism comprises a lamp holder 702, an illumination lamp 703 and a magnetic plate 705, the lamp holder 702 is connected to the turntable 105, the magnetic plate 705 is arranged at the lower end of the lamp holder 702, and the illumination lamp 703 is rotatably connected to the lamp holder 702.
The culture dish is periodically and reciprocally irradiated by simulating sunlight through the light of the lamp 703, the lamp 703 is mounted on the turntable 105 through the lamp holder 702, so that the turntable 105 rotates, and the lamp 703 is attracted and fixed through the magnetic plate 705, so that the lamp 703 can rotate along with the turntable 105 in a state of being parallel to the arm shaft 103 to form periodic irradiation on the culture dish.
Further, as shown in fig. 2-12:
the illumination mechanism further comprises an adjusting screw 701, the rotating disc 105 is provided with the adjusting screw 701 in a rotating mode, and the lamp holder 702 is in threaded connection with the adjusting screw 701.
By rotating the adjusting screw 701, the adjusting screw 701 drives the lamp holder 702 to move on the adjusting screw 701 through threads, so that the distance between the lamp holder 702 and the arm supporting shaft 103 is changed, the illumination distance is adjusted, and the device is suitable for illumination culture of different plant explants.
Further, as shown in fig. 2-12:
the illumination mechanism further comprises a lamp tube clamp 704, the lamp tube clamp 704 is fixed on the illumination lamp 703, and the adjusting screw 701 is in sliding connection with the lamp tube clamp 704 in a matching mode.
Through the arrangement of the lamp tube clamp 704, when the irradiation lamp 703 needs to be retracted, the lamp holder 702 is adjusted to the position with the minimum distance from the support arm shaft 103 through the adjusting screw 701, then the irradiation lamp 703 is rotated on the lamp holder 702, so that the lamp tube clamp 704 is driven to rotate until the lamp tube clamp 704 is clamped on the rotating button of the adjusting screw 701, the irradiation lamp 703 is perpendicular to the support arm shaft 103, and at the moment, the irradiation lamp 703 keeps a closed irradiation state; the culture dish is convenient to take and place from the supporting and placing mechanism in the state; at the same time, the lamp tube clamp 704 is clamped on the rotation knob of the adjusting screw 701, so that the adjusting screw 701 is fixed.
As shown in fig. 2-12:
the support mechanism comprises a support ring 104, a rotating ring 201, mounting long holes 203 and brackets, the support ring 104 is fixed on the support arm shaft 103, the rotating ring 201 rotates in the support ring 104, the lower end of the rotating ring 201 is uniformly provided with three mounting long holes 203, and the brackets are arranged in the three mounting long holes 203 in a sliding manner.
Three brackets are used for placing the culture dish, slowly rotate on the ring 104 through transmission change ring 201 to drive the culture dish and slowly rotate, cooperate the periodic irradiation of lamp 703, make the better illumination of cell in the culture dish cultivate.
Further, as shown in fig. 2-12:
the bracket comprises a sliding seat 301, a square rod 302, a lifting spring 303, a supporting plate 304 and a limiting column 306, wherein the supporting plate 304 is fixed at the upper end of the square rod 302, the limiting column 306 is fixed at the lower end of the square rod 302, the square rod 302 is provided with the sliding seat 301 in a sliding manner, the lifting spring 303 is arranged between the sliding seat 301 and the supporting plate 304, and the sliding seat 301 is connected with the installation long hole 203 in a sliding manner.
Lift to the culture dish is formed through three layer board 304, and through be equipped with the rubber slab on layer board 304, avoid the culture dish to slide on layer board 304, and take place to damage when avoiding culture dish and layer board 304 to contact, through lifting spring 303's setting, form and place back on layer board 304 at the culture dish, to the elastic support of culture dish, further form the protection to the culture dish, can also lift spring 303's distance according to the culture dish extrusion simultaneously, judge the measurement of culture solution interpolation in the culture dish.
Further, as shown in fig. 2-12:
the lifting mechanism further comprises a transmission disc 202 and a clamping screw 205, the transmission disc 202 is arranged at the lower end of the rotating ring 201 in a rotating mode, the transmission disc 202 is connected with three sliding seats 301 in a sliding mode, the clamping screw 205 is arranged on one sliding seat 301 in a rotating mode, and the clamping screw 205 is connected with the rotating ring 201 in a threaded mode.
By rotating the clamping screw 205, the position of the sliding seat 301 connected with the clamping screw 205 is controlled, so that the sliding seat 301 is close to or far away from the center of the rotating ring 201; when the position of the sliding seat 301 is controlled, the sliding seat 301 and the transmission disc 202 slide to enable the transmission disc 202 to rotate at the lower end of the rotating ring 201, and the long holes 203 are matched to enable the other two sliding seats 301 and the controlled sliding seat 301 to synchronously move, so that the purpose of controlling the positions of the three sliding seats 301 through the clamping screw 205 is achieved, the positions of the three support plates 304 are simultaneously controlled through the clamping screw 205, and the device can adapt to the placement of culture dishes with different sizes.
Further, as shown in fig. 2-12:
the lifting mechanism further comprises two clamping columns 305, and two clamping columns 305 are symmetrically fixed on the supporting plate 304 far away from the center end of the rotating ring 201.
When the position of three layer board 304 is controlled simultaneously through clamping screw 205, place the culture dish on three layer board 304 earlier, then clamping screw 205 is rotating, make three layer board 304 continue to be close to swivel 201 center to the outer border that makes the equal top of clamp post 305 on three layer board 304 culture dish, thereby further form the fixed to the culture dish, avoid the culture dish when rotating along with swivel 201 and carrying out illumination, the culture dish takes place to squint or landing, influences the cultivation of cell.
As shown in fig. 2-12:
the supporting device further comprises a supporting square rod 106, a transmission frame 601, a transmission wheel 602, a linkage wheel 603 and a linkage shaft rod 204, wherein the supporting square rod 106 is fixed on the base 101, the transmission frame 601 slides on the supporting square rod 106, one end of the transmission frame 601 rotates the transmission wheel 602, the other end of the transmission frame 601 rotates the linkage wheel 603, the transmission wheel 602 is in transmission connection with the linkage wheel 603, the linkage shaft rod 204 slides in the inner key of the linkage wheel 603, and the linkage shaft rod 204 is fixed at the center of the rotary ring 201.
The control telescopic rod is arranged between the transmission frame 601 and the base 101, the control telescopic rod is started to extend, the transmission frame 601 is pushed to ascend on the supporting square rod 106, then the transmission wheel 602 is contacted with the side surface of the rotary table 105, the reciprocating rotary table 105 starts to transmit the transmission wheel 602, the transmission wheel 602 transmits the linkage wheel 603 while rotating, the linkage wheel 603 drives the rotary ring 201 to rotate through the linkage shaft rod 204, so that the rotary table 105 rotates in a reciprocating manner, and the transmission rotary ring 201 drives the culture dish to rotate in a reciprocating manner;
and the distance between the contact position of the driving wheel 602 and the side surface of the turntable 105 and the arm supporting shaft 103 can be adjusted by controlling the telescopic rod, so that the transmission ratio is controlled.
As shown in fig. 2-12:
the supporting device further comprises a triangular prism plate 501, a vibration ring frame 502, round teeth 503, a threaded sleeve 504 and a cross rod frame 107, the triangular prism plate 501 slides at the lower end of the three limiting columns 306, the vibration ring frame 502 slides on the triangular prism plate 501, the vibration ring frame 502 is circumferentially and uniformly provided with the round teeth 503, the threaded sleeve 504 rotates at the center of the vibration ring frame 502 and is in threaded connection with the triangular prism plate 501, the linkage shaft rod 204 penetrates through the threaded sleeve 504, and the cross rod frame 107 is fixed on the support arm shaft 103.
Initially, the cross bar frame 107 is not in contact with the fillet teeth 503, when the rotating ring 201 rotates in a reciprocating manner, the rotating ring 201 drives the triangular prism plate 501 to rotate through the three limiting columns 306, the triangular prism plate 501 drives the vibration ring frame 502 to rotate in a reciprocating manner, when the culture dish needs to be vibrated, the threaded sleeve 504 is rotated, the threaded sleeve 504 is connected with the triangular prism plate 501 through threads, the threaded sleeve 504 drives the vibration ring frame 502 to ascend, so that the fillet teeth 503 are in contact with the cross bar frame 107, and the vibration ring frame 502 drives the culture dish to vibrate through the supporting plate 304 by matching with the elastic force of the lifting spring 303, so that the culture dish is convenient to disperse cells; and by rotating the threaded sleeve 504, the contact degree of the rounded teeth 503 with the cross bar frame 107 can be adjusted, thereby controlling the vibration degree.
As shown in fig. 2-12:
the supporting device further comprises a clamping limit plate 401, a clamping spring 402 and a clamping rod 403, the clamping limit plate 401 is fixed at the upper end of the clamping rod 403, the clamping rod 403 slides on the supporting plate 304, and the clamping spring 402 is arranged between the clamping limit plate 401 and the supporting plate 304.
Through the setting of pressing from both sides tight limiting plate 401, place on three layer board 304 at the culture dish, when three layer board 304 is further close to, the border of culture dish contacts with pressing from both sides tight limiting plate 401 earlier, form the extrusion to pressing from both sides tight limiting plate 401, thereby make and press from both sides tight limiting plate 401 along the border of culture dish upwards sliding, until pressing from both sides tight limiting plate 401 and receiving the border that presss from both sides tight culture dish by the elastic force influence of clamping spring 402, continue to make three layer board 304 be close to, make the border top of culture dish press from both sides post 305, thereby it is fixed to the circumference of culture dish to form through post 305, it is fixed to the upper and lower direction of culture dish through three clamp limiting plate 401, avoid when shaking the culture dish, the culture dish drops.
The cell culture method is applied to the differentiation culture of the vegetative cells.
The plant cell differentiation culture system can perform periodic illumination more needed, and can adjust illumination according to different plants better through the supporting device.

Claims (10)

1. A method of cell culture, comprising: the method comprises the following steps:
s1, taking the explant for disinfection, and then directly separating cells from the explant;
s2, placing the separated cells in a culture dish filled with a culture medium;
s3, placing the culture dish on a supporting device for illumination culture;
s4, controlling the culture temperature and appropriately supplementing fresh culture solution.
2. A cell culture method according to claim 1, wherein: the supporting device comprises a base (101), a supporting plate (102), a supporting arm shaft (103), a supporting mechanism, a turntable (105) and an illumination mechanism, wherein the supporting plate (102) is fixed on the base (101), the supporting arm shaft (103) is transversely arranged at the upper end of the supporting plate (102), one end of the supporting arm shaft (103) is connected with the supporting mechanism for placing a culture dish, the turntable (105) rotates on the supporting arm shaft (103), and the illumination mechanism is connected on the turntable (105).
3. A cell culture method according to claim 2, wherein: the illumination mechanism comprises a lamp holder (702), an illuminating lamp (703) and a magnetic plate (705), the lamp holder (702) is connected to the rotary disc (105), the magnetic plate (705) is arranged at the lower end of the lamp holder (702), and the illuminating lamp (703) rotates on the lamp holder (702).
4. A cell culture method according to claim 3, wherein: the illumination mechanism further comprises an adjusting screw rod (701), the adjusting screw rod (701) rotates on the rotary plate (105), and the adjusting screw rod (701) is in threaded connection with the lamp holder (702).
5. A cell culture method according to claim 4, wherein: the illumination mechanism further comprises a lamp clamp (704), the lamp clamp (704) is fixed on the illumination lamp (703), and the lamp clamp (704) is in sliding connection with the adjusting screw rod (701) in a matched mode.
6. A cell culture method according to claim 2, wherein: the support mechanism comprises a support ring (104), a rotating ring (201), installation long holes (203) and a bracket, wherein the support ring (104) is fixed on a supporting arm shaft (103), the rotating ring (201) rotates in the support ring (104), the lower end of the rotating ring (201) is uniformly provided with three installation long holes (203), and the bracket is arranged in the three installation long holes (203) in a sliding mode.
7. A cell culture method according to claim 6, wherein: the bracket comprises a sliding seat (301), a square rod (302), a lifting spring (303), a supporting plate (304) and a limiting column (306), wherein the supporting plate (304) is fixed at the upper end of the square rod (302), the limiting column (306) is fixed at the lower end of the square rod (302), the sliding seat (301) slides on the square rod (302), the lifting spring (303) is arranged between the sliding seat (301) and the supporting plate (304), and the sliding seat (301) slides in an installation long hole (203).
8. A cell culture method according to claim 7, wherein: the lifting mechanism further comprises a transmission disc (202) and a clamping screw (205), the transmission disc (202) rotates at the lower end of the rotating ring (201) and is in sliding connection with the three sliding seats (301), and the clamping screw (205) rotates on one of the sliding seats (301) and is in threaded connection with the rotating ring (201).
9. A cell culture method according to claim 8, wherein: the supporting mechanism further comprises clamping columns (305), and two clamping columns (305) are symmetrically fixed on the supporting plate (304) far away from the center end of the rotating ring (201).
10. Use of a cell culture method according to claim 1 in the differentiation culture of vegetative cells.
CN202111432252.8A 2021-11-29 2021-11-29 Cell culture method and application thereof Withdrawn CN114107166A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202111432252.8A CN114107166A (en) 2021-11-29 2021-11-29 Cell culture method and application thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202111432252.8A CN114107166A (en) 2021-11-29 2021-11-29 Cell culture method and application thereof

Publications (1)

Publication Number Publication Date
CN114107166A true CN114107166A (en) 2022-03-01

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CN202111432252.8A Withdrawn CN114107166A (en) 2021-11-29 2021-11-29 Cell culture method and application thereof

Country Status (1)

Country Link
CN (1) CN114107166A (en)

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Application publication date: 20220301