CN114107020A - A device for observing microbial community - Google Patents

A device for observing microbial community Download PDF

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Publication number
CN114107020A
CN114107020A CN202111291857.XA CN202111291857A CN114107020A CN 114107020 A CN114107020 A CN 114107020A CN 202111291857 A CN202111291857 A CN 202111291857A CN 114107020 A CN114107020 A CN 114107020A
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CN
China
Prior art keywords
wall
observation
lifting
rod
observing
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Pending
Application number
CN202111291857.XA
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Chinese (zh)
Inventor
邢承华
蒋红英
王新洁
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Jinhua Polytechnic
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Jinhua Polytechnic
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Priority to CN202111291857.XA priority Critical patent/CN114107020A/en
Publication of CN114107020A publication Critical patent/CN114107020A/en
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/22Transparent or translucent parts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/48Holding appliances; Racks; Supports
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48Automatic or computerized control

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Computer Hardware Design (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a device for observing microbial colonies, which comprises an incubator matrix, wherein a top plate is arranged on the outer wall of the top of the incubator matrix, a sampling mechanism is fixedly installed on the outer wall of the bottom of the top plate, an observation chamber and a culture chamber are arranged in the incubator matrix, a third lifting door plate is arranged at the bottom of the inner wall of the incubator matrix, and a lifting mechanism is arranged at the bottom of the inner wall of the observation chamber. According to the invention, the operation control panel, the observation instrument, the lifting mechanism and the sampling mechanism are matched with each other, the short rod moves to the highest position, the observation product on the placing plate gradually approaches to the objective lens of the observation instrument in the process, at the moment, a worker can observe the object in the culture medium through the observation instrument, if the worker needs to take out the observation product according to the observation result, the first lifting door plate can be opened and the sample can be taken out, and if the worker does not need to take out the observation product, the first lifting door plate can not be opened, so that the air exchange between the interior of the device and the exterior is reduced.

Description

A device for observing microbial community
Technical Field
The invention relates to the technical field of microbial culture, in particular to a device for observing microbial colonies.
Background
In the process of microbial cultivation, a temperature-controlled incubator is required for cultivation, so that a favorable environment is provided for microbial growth and propagation, most of the existing incubators are designed in a multilayer mode, and meanwhile, an illuminating device is arranged in the incubator.
When the growth state of microorganisms needs to be observed, the existing device for observing the microbial colonies needs to take out a culture medium from an incubator, observe the culture medium under a microscope, and record the observation result through electronic equipment. However, in the use process of the existing device, the culture medium needs to be placed outside the incubator for a long time, and the temperature in the incubator is easy to generate large fluctuation by opening the incubator for many times, so that the growth of microorganisms is not facilitated.
Disclosure of Invention
The invention aims to solve the defects in the prior art and provides a device for observing microbial colonies.
The invention provides a device for observing microbial colonies, which comprises an incubator base body, wherein a top plate is arranged on the outer wall of the top of the incubator base body, a sampling mechanism is fixedly arranged on the outer wall of the bottom of the top plate, an observation chamber and a culture chamber are arranged inside the incubator base body, a third lifting door plate is arranged at the bottom of the inner wall of the incubator base body, a lifting mechanism is arranged at the bottom of the inner wall of the observation chamber, a first lifting door plate and a second lifting door plate are arranged on one side of the incubator base body, a placing frame is arranged on the outer wall of the top of the incubator base body, the middle of the placing frame is in a shape like a Chinese character hui, a mounting seat is arranged on the inner wall of one side of the placing frame, an observation instrument is arranged on the mounting seat, an observation window is arranged in the middle of the placing frame, an objective lens of the observation instrument is positioned inside the observation chamber, and an eyepiece of the observation instrument is positioned above the observation window, and a display and an operation control panel are fixedly arranged on the outer wall of the top plate.
As a still further scheme of the invention: the lifting mechanism consists of a linear motor, a linear clamping rail, a first electric telescopic rod, a first connecting arm, a mechanical arm, a second electric telescopic rod, a rotating rod and an electric clamping jaw.
As a still further scheme of the invention: the bottom outer wall of sharp card rail fixed mounting and roof, linear electric motor sliding connection is in the bottom of sharp card rail, first electric telescopic handle fixed mounting and linear electric motor's bottom outer wall, second electric telescopic handle's one end is provided with L type connecting rod, the dwang passes through the bearing and rotates the outer wall of connecting in L type pole, electric clamping jaw installs in the outer wall of dwang.
As a still further scheme of the invention: the arm comprises first connecting arm, rotation motor and second linking arm, first connecting arm fixed mounting is in first electric telescopic handle's bottom outer wall, second linking arm swing joint is in one side outer wall of first connecting arm, rotate the motor and install in one side outer wall of second linking arm.
As a still further scheme of the invention: the lifting mechanism consists of a short rod, a supporting block, a sliding rod, a driven gear, a driving gear, a connecting rod, a first supporting frame, a second supporting frame, a lifting motor, a placing plate and a guide block.
As a still further scheme of the invention: first carriage fixed mounting is in the inner wall bottom of observation room, promote motor fixed mounting in one side inner wall of observation room, the driving gear sets up in the both ends of connecting rod, the both ends of driving gear pass through the bearing with first carriage and rotate the contact, and the output shaft that promotes the motor passes through the shaft coupling and is connected with the driving gear transmission, driven gear and driving gear meshing, the both ends of quarter butt are passed through the bearing and are rotated with the second carriage and be connected, driven gear all welds in the outer wall of quarter butt.
As a still further scheme of the invention: the quantity of supporting shoe is two, the supporting shoe sets up between two second carriage, the guide block all welds in the outer wall of second carriage, slide bar and guide block sliding contact, driving gear and quarter butt all are located eccentric position, the top outer wall fixed mounting of supporting shoe has places the board.
As a still further scheme of the invention: the top end of the slide bar is fixedly provided with a limiting block.
As a still further scheme of the invention: a monitoring probe is installed inside the culture chamber.
The beneficial effects of the invention are as follows:
1. the operation control panel, the observation instrument, the lifting mechanism and the sampling mechanism are matched with each other, the short rod moves to the highest position, the observation product on the placing plate gradually approaches to the objective lens of the observation instrument in the process, at the moment, a worker can observe the object in the culture medium through the observation instrument, if the worker needs to take out the observation product according to the observation result, the first lifting door plate can be opened and the sample can be taken out, and if the worker does not need to take out the observation product, the first lifting door plate can not be opened, so that the air exchange between the interior of the device and the exterior is reduced; because the observation window is arranged on the placing frame, workers can roughly know the appearance of the sample through the observation window.
2. The incubator can display relevant data inside the incubator matrix through the display, and displays the picture transmitted by the monitoring probe, so that workers can be assisted to operate the electronic equipment conveniently.
3. The lifting process of the placing plate is divided into two stages, so that a sample can be conveniently placed at a proper position, and the electric clamping jaw is prevented from colliding with an objective lens of an observer; the dwang and the L type pole swing joint that set up from this under the dead weight effect of electronic clamping jaw, can keep the vertical downwards of clamping jaw clock of electronic clamping jaw, do not receive the influence of arm state.
Drawings
FIG. 1 is a schematic view, partially in section, of an apparatus for observing colonies of microorganisms according to the present invention;
FIG. 2 is a schematic view of the overall structure of a device for observing colonies of microorganisms according to the present invention;
FIG. 3 is a schematic structural diagram of a sampling mechanism of a device for observing microbial colonies according to the present invention;
FIG. 4 is a schematic structural diagram of a lifting mechanism of the device for observing microbial colonies provided by the invention.
In the figure: the device comprises a display 1, a mounting seat 2, a placing frame 3, a first lifting door plate 4, a top plate 5, an operation control plate 6, an incubator base body 7, a second lifting door plate 8, a linear motor 9, a linear clamping rail 10, a first electric telescopic rod 11, a first connecting arm 12, a rotating motor 13, a second connecting arm 14, a second electric telescopic rod 15, a rotating rod 16, an electric clamping jaw 17, a short rod 18, a supporting block 19, a sliding rod 20, a driven gear 21, a driving gear 22, a connecting rod 23, a first supporting frame 24, a second supporting frame 25, a third lifting door 26, an observation room 27, a limiting block 28, a culture room 29, a lifting motor 30, a placing plate 31 and a guide block 32.
Detailed Description
Reference will now be made in detail to embodiments of the present patent, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary only for the purpose of explaining the present patent and are not to be construed as limiting the present patent.
In the description of this patent, it is to be understood that the terms "center," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in the orientations and positional relationships indicated in the drawings for the convenience of describing the patent and for the simplicity of description, and are not intended to indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and are not to be considered limiting of the patent.
In the description of this patent, it is noted that unless otherwise specifically stated or limited, the terms "mounted," "connected," and "disposed" are to be construed broadly and can include, for example, fixedly connected, disposed, detachably connected, disposed, or integrally connected and disposed. The specific meaning of the above terms in this patent may be understood by those of ordinary skill in the art as appropriate.
Referring to fig. 1-4, a device for observing microbial colonies comprises an incubator base 7, a top plate 5 is arranged on the outer wall of the top of the incubator base 7, a sampling mechanism is fixedly arranged on the outer wall of the bottom of the top plate 5, an observation chamber 27 and an incubation chamber 29 are arranged inside the incubator base 7, a third lifting door plate 26 is arranged on the bottom of the inner wall of the incubator base 7, a lifting mechanism is arranged on the bottom of the inner wall of the observation chamber 27, a first lifting door plate 4 and a second lifting door plate 8 are arranged on one side of the incubator base 7, a placing frame 3 is arranged on the outer wall of the top of the incubator base 7, the middle of the placing frame 3 is in a shape like a Chinese character 'hui', a mounting seat 2 is arranged on the inner wall of one side of the placing frame 3, an observation instrument is arranged on the mounting seat 2, an observation window is arranged in the middle of the placing frame 3, an objective lens of the observation instrument is positioned inside the observation chamber 27, and an eyepiece of the observation instrument is positioned above the observation window, top outer wall fixed mounting of roof 5 has display 1 and operation control panel 6, through being provided with operation control panel, the visualizer, hoist mechanism and sampling mechanism mutually support, the quarter butt removes the highest position, the observation article on the place board is close the objective of visualizer gradually at this in-process, the staff can observe the article in the culture medium through the visualizer this moment, if need take out the observation article according to observation result staff, then can open first lift door plant and take out the sample, if need not take out, then can not open first lift door plant, with this inside and external air exchange of reduction device.
In the invention, the lifting mechanism consists of a linear motor 9, a linear clamping rail 10, a first electric telescopic rod 11, a first connecting arm 12, a mechanical arm, a second electric telescopic rod 15, a rotating rod 16 and an electric clamping jaw 17.
According to the invention, the linear clamping rail 10 is fixedly arranged on the outer wall of the bottom of the top plate 5, the linear motor 9 is connected to the bottom of the linear clamping rail 10 in a sliding manner, the first electric telescopic rod 11 is fixedly arranged on the outer wall of the bottom of the linear motor 9, the L-shaped connecting rod is arranged at one end of the second electric telescopic rod 15, the rotating rod 16 is rotatably connected to the outer wall of the L-shaped rod through a bearing, the electric clamping jaw 17 is arranged on the outer wall of the rotating rod 16, and the rotating rod 16 is movably connected with the L-shaped rod, so that under the self-weight action of the electric clamping jaw 17, the clamping jaw clock of the electric clamping jaw 17 can be kept vertically downward without being influenced by the state of a mechanical arm.
According to the invention, the mechanical arm comprises a first connecting arm 12, a rotating motor 13 and a second connecting arm 14, wherein the first connecting arm 12 is fixedly arranged on the outer wall of the bottom of the first electric telescopic rod 11, the second connecting arm 14 is movably connected to the outer wall of one side of the first connecting arm 12, the rotating motor 13 is arranged on the outer wall of one side of the second connecting arm 14, and the set rotating motor 13 can drive an output shaft of the rotating motor 13 of the second connecting arm 14 to rotate by taking the output shaft as a central line, so that the right-angle rotation of the mechanical arm is realized.
In the invention, the lifting mechanism is composed of a short rod 18, a supporting block 19, a sliding rod 20, a driven gear 21, a driving gear 22, a connecting rod 23, a first supporting frame 24, a second supporting frame 25, a lifting motor 30, a placing plate 31 and a guide block 32.
According to the invention, a first supporting frame 24 is fixedly arranged at the bottom of the inner wall of an observation chamber 27, a lifting motor 30 is fixedly arranged on the inner wall of one side of the observation chamber 27, driving gears 22 are arranged at two ends of a connecting rod 23, two ends of the driving gear 22 are in rotary contact with the first supporting frame 24 through bearings, an output shaft of the lifting motor 30 is in transmission connection with the driving gear 22 through a coupler, a driven gear 21 is meshed with the driving gear 22, two ends of a short rod 18 are in rotary connection with a second supporting frame 25 through bearings, and the driven gear 21 is fixedly connected to the outer wall of the short rod 18.
In the invention, the number of the supporting blocks 19 is two, the supporting blocks 19 are arranged between the two second supporting frames 25, the guide blocks 32 are fixedly connected to the outer walls of the second supporting frames 25, the sliding rods 20 are in sliding contact with the guide blocks 32, the driving gear 22 and the short rod 18 are both located at eccentric positions, and the placing plate 31 is fixedly arranged on the outer wall of the top of the supporting block 19.
In the present invention, the top ends of the sliding rods 20 are all fixedly provided with a limiting block 28.
In the invention, the monitoring probe is arranged in the culture chamber 29, and the display 1 can display the data detected by the related sensor in the culture box substrate 7 and display the picture transmitted by the monitoring probe, thereby being convenient for assisting the staff to operate the electronic equipment.
When the lifting device is used, a worker controls the linear motor 9 to move along the linear clamping rail 10 through the arranged operation control panel 6, then controls the first electric telescopic rod 11 to extend to a proper position, rotates the motor 13 to act so that the second connecting arm 14 is in a vertical state, so that the electric clamping jaw 17 falls above a culture product to be taken, after the electric clamping jaw 17 clamps an observed product, rotates the motor 13 to act again so that the second connecting arm 14 is in a horizontal position, the first electric telescopic rod 11 retracts properly, the linear motor 9 drives the electric clamping jaw 17 of the lifting mechanism to be positioned at the third lifting door panel 26, the worker controls the lifting motor 30 to act, in the process that the lifting motor 30 drives the driving gear 22 to rotate, the guide block 32 slides along the outer wall of the sliding rod 20, when the short rod 18 runs to a proper position, the lifting motor 30 stops rotating, at the moment, the third lifting door panel 26 descends, the second electric telescopic rod 15 is properly extended, the electric clamping jaw 17 is opened to place the observed object on the placing plate 31, then the second electric telescopic rod 15 retracts, the third lifting door plate 26 is lifted, the lifting motor 30 rotates again, so that the short rod 18 moves to the highest position, the observed object on the placing plate 31 gradually approaches to an objective lens of the observer in the process, at the moment, a worker can observe the object in the culture medium through the observer, and if the worker needs to take out the observed object according to the observation result, the first lifting door plate 4 can be opened and the sample can be taken out; if the first lifting door panel is not required to be taken out, the first lifting door panel 4 is not required to be opened, so that the air exchange between the interior of the device and the external air is reduced; because install the observation window on the rack 3, the observation window adopts transparent material to make, is sealing contact between observation window and the rack 3, and the staff can roughly know the outward appearance of sample through the observation window to this solves the eyepiece observation scope and is little and can't observe the drawback of the whole outward appearance of sample.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims (9)

1. The device for observing microbial colonies comprises an incubator base body (7) and is characterized in that a top plate (5) is arranged on the outer wall of the top of the incubator base body (7), a sampling mechanism is fixedly mounted on the outer wall of the bottom of the top plate (5), an observation chamber (27) and an incubation chamber (29) are arranged inside the incubator base body (7), a third lifting door panel (26) is arranged at the bottom of the inner wall of the incubator base body (7), a lifting mechanism is arranged at the bottom of the inner wall of the observation chamber (27), a first lifting door panel (4) and a second lifting door panel (8) are arranged on one side of the incubator base body (7), a placing frame (3) is arranged on the outer wall of the top of the incubator base body (7), the middle of the placing frame (3) is in a shape of a Chinese character 'hui', a mounting seat (2) is arranged on the inner wall of one side of the placing frame (3), and the installation seat (2) is provided with an observation instrument, the middle of the placement frame (3) is provided with an observation window, an objective lens of the observation instrument is positioned in the observation chamber (27), an eyepiece of the observation instrument is positioned above the observation window, and the outer wall of the top plate (5) is fixedly provided with a display (1) and an operation control panel (6).
2. The device for observing colonies of microorganisms according to claim 1, wherein said lifting mechanism is composed of a linear motor (9), a linear clamping rail (10), a first electric telescopic rod (11), a first connecting arm (12), a mechanical arm, a second electric telescopic rod (15), a rotating rod (16), and an electric clamping jaw (17).
3. The device for observing microbial colonies as claimed in claim 2, wherein the linear clamping rail (10) is fixedly installed on the outer wall of the bottom of the top plate (5), the linear motor (9) is slidably connected to the bottom of the linear clamping rail (10), the first electric telescopic rod (11) is fixedly installed on the outer wall of the bottom of the linear motor (9), an L-shaped connecting rod is arranged at one end of the second electric telescopic rod (15), the rotating rod (16) is rotatably connected to the outer wall of the L-shaped rod through a bearing, and the electric clamping jaw (17) is installed on the outer wall of the rotating rod (16).
4. The device for observing colonies of microorganisms according to claim 2, wherein said mechanical arm comprises a first connecting arm (12), a rotating motor (13) and a second connecting arm (14), said first connecting arm (12) is fixedly mounted on the outer wall of the bottom of the first electric telescopic rod (11), said second connecting arm (14) is movably connected to the outer wall of one side of the first connecting arm (12), and said rotating motor (13) is mounted on the outer wall of one side of the second connecting arm (14).
5. The device for observing colonies of microorganisms according to claim 1, wherein the lifting mechanism is composed of a short bar (18), a support block (19), a slide bar (20), a driven gear (21), a driving gear (22), a connecting bar (23), a first support frame (24), a second support frame (25), a lifting motor (30), a placing plate (31) and a guide block (32).
6. The device for observing microbial colonies as claimed in claim 5, wherein the first supporting frame (24) is fixedly installed at the bottom of the inner wall of the observation chamber (27), the lifting motor (30) is fixedly installed at the inner wall of one side of the observation chamber (27), the driving gear (22) is arranged at two ends of the connecting rod (23), two ends of the driving gear (22) are in rotational contact with the first supporting frame (24) through a bearing, an output shaft of the lifting motor (30) is in transmission connection with the driving gear (22) through a coupler, the driven gear (21) is meshed with the driving gear (22), two ends of the short rod (18) are in rotational connection with the second supporting frame (25) through a bearing, and the driven gear (21) is fixedly connected to the outer wall of the short rod (18).
7. The device for observing colonies of microorganisms according to claim 5, wherein the number of the support blocks (19) is two, the support blocks (19) are disposed between two second support frames (25), the guide blocks (32) are fixedly connected to the outer walls of the second support frames (25), the slide bars (20) are in sliding contact with the guide blocks (32), the driving gears (22) and the short bars (18) are located at eccentric positions, and the top outer walls of the support blocks (19) are fixedly provided with the placing plates (31).
8. The device for observing colonies of microorganisms according to claim 7, wherein a stopper (28) is fixedly mounted on each top end of the slide bar (20).
9. A device for observing colonies of microorganisms according to claim 8, wherein the culture chamber (29) is internally equipped with a monitoring probe.
CN202111291857.XA 2021-11-03 2021-11-03 A device for observing microbial community Pending CN114107020A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202111291857.XA CN114107020A (en) 2021-11-03 2021-11-03 A device for observing microbial community

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202111291857.XA CN114107020A (en) 2021-11-03 2021-11-03 A device for observing microbial community

Publications (1)

Publication Number Publication Date
CN114107020A true CN114107020A (en) 2022-03-01

Family

ID=80380462

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202111291857.XA Pending CN114107020A (en) 2021-11-03 2021-11-03 A device for observing microbial community

Country Status (1)

Country Link
CN (1) CN114107020A (en)

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