CN114106979A - Bioreactor capable of supplying illumination based on microalgae density - Google Patents
Bioreactor capable of supplying illumination based on microalgae density Download PDFInfo
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- CN114106979A CN114106979A CN202111386396.4A CN202111386396A CN114106979A CN 114106979 A CN114106979 A CN 114106979A CN 202111386396 A CN202111386396 A CN 202111386396A CN 114106979 A CN114106979 A CN 114106979A
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- 238000005286 illumination Methods 0.000 title claims abstract description 38
- 238000006243 chemical reaction Methods 0.000 claims abstract description 68
- 238000005273 aeration Methods 0.000 claims description 38
- 230000000670 limiting effect Effects 0.000 claims description 31
- 230000029553 photosynthesis Effects 0.000 claims description 21
- 238000010672 photosynthesis Methods 0.000 claims description 21
- 238000007599 discharging Methods 0.000 claims description 18
- 238000003756 stirring Methods 0.000 claims description 18
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 5
- 239000001301 oxygen Substances 0.000 claims description 5
- 229910052760 oxygen Inorganic materials 0.000 claims description 5
- 241000195493 Cryptophyta Species 0.000 claims description 4
- 230000000903 blocking effect Effects 0.000 claims description 3
- 230000005540 biological transmission Effects 0.000 claims 1
- 230000009471 action Effects 0.000 abstract description 4
- 239000000243 solution Substances 0.000 description 17
- 238000000034 method Methods 0.000 description 7
- 230000008569 process Effects 0.000 description 6
- 230000001681 protective effect Effects 0.000 description 5
- 230000009286 beneficial effect Effects 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 125000004122 cyclic group Chemical group 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 230000000243 photosynthetic effect Effects 0.000 description 3
- 230000008859 change Effects 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000036961 partial effect Effects 0.000 description 2
- 230000003313 weakening effect Effects 0.000 description 2
- 239000002537 cosmetic Substances 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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Abstract
The invention relates to a bioreactor, in particular to a bioreactor capable of supplying light based on microalgae density, which comprises a first support frame, a second support frame, a support bottom frame, a transparent reaction frame and the like; the top of the first support frame is connected with a second support frame, the second support frame is connected with a support bottom frame, and the support bottom frame is connected with a transparent reaction frame through bolts. When the density of the microalgae in the transparent reaction frame at the initial reaction stage is low, the light irradiated to the transparent reaction frame is weakened due to the action of the refraction light-transmitting plate, and when the density of the microalgae in the transparent reaction frame at the later reaction stage is high, the light on the lamp tube is not refracted by the refraction light-transmitting plate, so that the illumination is increased, and the purpose of automatically adjusting the supply amount of the illumination according to the density of the microalgae is realized.
Description
Technical Field
The invention relates to a bioreactor, in particular to a bioreactor capable of supplying light based on microalgae density.
Background
Microalgae is a micro algae group whose form can be identified under a microscope, is a photoautotrophic microorganism, has high glycerol content in algae cells, is a high-quality cosmetic raw material, and is an organic intermediate with wide application in chemical industry, light industry and pharmaceutical industry.
Microalgae cells can grow and proliferate in large quantity under certain conditions, the method for culturing microalgae can be divided into closed culture and open culture, the open culture is easily polluted and high-quality microalgae cannot be obtained, the closed culture enables the culture process to be controllable, but the existing closed culture method still has certain defects, the existing closed culture process is accompanied with the propagation of the microalgae, the microalgae in the deep layer is subjected to weak illumination or no light, the photosynthesis of the microalgae is influenced, dissolved oxygen in culture solution containing the microalgae can be gradually increased along with the culture of the microalgae, the photosynthesis of the microalgae can be inhibited at the later stage, the photosynthesis efficiency of the microalgae is caused, meanwhile, the density of the microalgae can be increased in the culture process, when the density of the microalgae is small, the required illumination is less, and along with the propagation of the microalgae, the density can be increased, more illumination is required at the moment, however, the prior art is difficult to change the illumination amount according to the density of the microalgae, and is not beneficial to culturing the microalgae.
Disclosure of Invention
Accordingly, it is necessary to provide a bioreactor capable of effectively avoiding a photosynthetic blind area, sufficiently and effectively improving photosynthetic efficiency of microalgae, and automatically adjusting a supply amount of light according to a density of the microalgae, which can supply light based on the density of the microalgae, so as to solve the problems that the photosynthetic blind area affects the photosynthesis of the microalgae, the photosynthesis efficiency of the microalgae is low, and it is difficult to change the amount of light according to the density of the microalgae in the prior art provided in the background art.
The technical scheme is as follows: the utility model provides a bioreactor that illumination was supplied with can be based on little algae density, including first support frame, second support frame, support underframe, transparent reaction frame, motor, first support plectane, first revolving rack, second support plectane, ejection of compact frame, ball valve, aeration subassembly and stirring subassembly:
the top of the first support frame is connected with a second support frame;
the second support frame is connected with the support bottom frame;
the transparent reaction frame is connected to the supporting bottom frame through bolts and communicated with the supporting bottom frame, and the transparent reaction frame is cylindrical;
the motor is fixedly arranged on the first support frame and used for driving the equipment to operate;
the transparent reaction frame is connected with the first supporting circular plate;
the first rotating frame is rotatably connected to the first supporting circular plate;
the first rotating frame is rotatably connected with a first supporting circular plate;
the discharging frame is connected to the outer bottom of the supporting bottom frame and is communicated with the supporting bottom frame, and the discharging frame is used for discharging the well-reacted culture solution containing the microalgae;
the ball valve is rotatably connected to the discharging frame and used for temporarily blocking the discharging frame;
the aeration component is arranged on the supporting bottom frame and used for removing dissolved oxygen generated by photosynthesis of the microalgae in time;
the stirring component is arranged on the transparent reaction frame and used for improving the photosynthesis efficiency of the microalgae.
Optionally, the outer wall of the transparent reaction frame is of a symmetrical concave structure, and the light quantity which can enter the transparent reaction frame is increased, so that the light blind area of the microalgae is reduced.
Optionally, the aeration subassembly is including the frame of admitting air, aeration frame, fixed arc and rotation circle frame, and the bottom switch-on has the frame of admitting air outside the support underframe, and the inside hookup of support underframe has the aeration frame, is the annular distributing type hookup on the aeration frame and has six fixed arcs, and the last rotation type of aeration frame is connected with the rotation circle frame, rotates the circle frame and closely laminates with fixed arc.
Optionally, the stirring assembly comprises a vibrating block, a stirring plate, a gear ring and a first straight gear, the three vibrating blocks are connected to the inner wall of the transparent reaction frame in a linear distributed manner, the stirring plate is fixedly connected to the bottom of the first rotating frame, the gear ring is connected to the outside of the first rotating frame, the first straight gear is connected to one end of an output shaft of the motor, and the first straight gear is meshed with the gear ring.
Optionally, the lamp further comprises an illumination assembly, the illumination assembly is arranged on the first rotating frame and comprises a reflecting frame, a lamp tube and a concave lens, the bottom of the first rotating frame is symmetrically connected with the reflecting frame, the lamp tube is connected inside the reflecting frame, and the concave lens is symmetrically connected on the reflecting frame.
Optionally, the illumination device further comprises an illumination intensity control assembly, the illumination intensity control assembly is arranged on the reflection frame, the illumination intensity control assembly comprises a refraction light-passing plate, a third support frame, a slotting pushing frame, a first spring, a limiting rod, a limiting round frame and a second spring, the reflection frame is symmetrically and slidably connected with the refraction light-passing plate, the third support frame is connected to the outer bottom of the reflection frame, the third support frame is slidably connected with the slotting pushing frame, the slotting pushing frame is in limiting fit with the refraction light-passing plate, the first spring is connected between the slotting pushing frame and the third support frame, the limiting rod is symmetrically connected on the second support frame, the limiting round frame is connected between the two limiting rods in a sliding mode, the limiting round frame is in contact with the slotting pushing frame, and the second spring is symmetrically connected between the limiting round frame and the second support frame.
Optionally, the surface of the refraction light-transmitting plate is in a plurality of convex structures, and the convex structures are used for reflecting part of light rays and reducing the light-transmitting amount, so that the function of weakening the light rays irradiated on the transparent reaction frame is achieved.
Optionally, the air intake device further comprises a pushing assembly, the pushing assembly is arranged on the air intake frame, the pushing assembly comprises a second rotating frame, a second straight gear, a third rotating frame, a third straight gear, a fourth supporting frame, an inclined plane ring frame, a column clamping frame, a third spring and a slotting rotating frame, the second rotating frame is rotatably connected inside the air intake frame, the second straight gear is connected on the second rotating frame in a coupling mode, the second straight gear is located outside the air intake frame, the third rotating frame is rotatably connected outside the air intake frame, the third rotating frame is connected with the third straight gear through a bolt, the third straight gear is meshed with the second straight gear, the fourth supporting frame is connected on the second supporting frame, the inclined plane ring frame is slidably connected on the fourth supporting frame, the inclined plane ring frame is in contact with the third rotating frame, the column clamping frame is connected on the inclined plane ring frame, the third spring is connected between the inclined plane ring frame and the fourth supporting frame, the slotting rotating frame is rotatably connected on the second supporting frame, the slotting rotating frame is in limit fit with the clamping column frame.
Optionally, still including the protective assembly, the protective assembly is located on first support frame, the protective assembly is including the guard plate, the fourth rotating turret, the fifth support frame, slip fluting board and fluting frame, it has six guard plates to be the hookup of cyclic annular distributing type on the circle frame to rotate, it has the fourth rotating turret to slide formula to be connected on the support underframe, the fourth rotating turret couples with the circle frame that rotates, first support frame top hookup has the fifth support frame, it has the slip fluting board to slide formula on the fifth support frame, slip fluting board and the spacing cooperation of fourth rotating turret, it has the fluting frame to link on the slip fluting board, fluting frame and the spacing cooperation of ball valve.
The invention has the beneficial effects that:
the shaking block enables the culture solution containing the microalgae in the transparent reaction frame to fluctuate up and down, so that the longitudinal distribution of the culture solution containing the microalgae in the transparent reaction frame is changed, the photosynthesis efficiency of the microalgae can be effectively improved, meanwhile, the lamp tube can rotate around the transparent reaction frame, the photosynthesis efficiency of the microalgae is further improved, and the effect of fully and effectively improving the photosynthesis efficiency of the microalgae is achieved.
The concave lens can refract light, so that the light can irradiate the deep layer inside the microalgae in the transparent reaction frame, the light can be sufficiently irradiated by the light, the photosynthesis efficiency of the microalgae is improved, the photosynthesis blind area is effectively avoided, and the integral photosynthesis of the system is improved.
When the density of the microalgae in the transparent reaction frame at the initial reaction stage is low, the light irradiated to the transparent reaction frame is weakened due to the action of the refraction light-transmitting plate, and when the density of the microalgae in the transparent reaction frame at the later reaction stage is high, the light on the lamp tube is not refracted by the refraction light-transmitting plate, so that the illumination is increased, and the purpose of automatically adjusting the supply amount of the illumination according to the density of the microalgae is realized.
Before discharging the culture solution containing the microalgae in the transparent reaction frame, the protective plate and the fixed arc plate can cover the aeration frame together to prevent the aeration frame from being blocked by the microalgae, so that the equipment is convenient to reuse, and the effect of effectively preventing the aeration frame from being blocked by the microalgae is achieved.
Drawings
Fig. 1 is a schematic perspective view of a first embodiment of the present invention.
Fig. 2 is a schematic perspective view of a second embodiment of the present invention.
FIG. 3 is a schematic view of a first partial body structure according to the present invention.
Fig. 4 is a schematic view of a partially cut-away perspective structure of an aeration assembly according to the present invention.
Fig. 5 is a schematic perspective view of the first rotating frame according to the present invention.
FIG. 6 is a schematic view of a second partial body structure according to the present invention.
Fig. 7 is a schematic perspective view of the ball valve of the present invention.
Fig. 8 is a schematic perspective view of the illumination assembly of the present invention.
FIG. 9 is a schematic view of a first partially assembled body of the illumination intensity control assembly according to the present invention.
FIG. 10 is a schematic view of a second partially assembled body of the illumination intensity control assembly according to the present invention.
FIG. 11 is a perspective view of a third portion of the illumination intensity control assembly according to the present invention.
Fig. 12 is a schematic perspective view of a refractive light-transmitting plate according to the present invention.
FIG. 13 is a schematic view of a first partially assembled body of the pushing assembly of the present invention.
Fig. 14 is a partially cut-away perspective view of the pushing assembly of the present invention.
FIG. 15 is a schematic view of a second partially assembled body of the pushing assembly of the present invention.
Fig. 16 is a schematic view of a first partially assembled body of the shield assembly of the present invention.
Fig. 17 is a schematic view of a second partially assembled body of the shield assembly of the present invention.
Reference numbers in the drawings: 1_ first support frame, 21_ second support frame, 22_ support bottom frame, 23_ transparent reaction frame, 24_ motor, 25_ first support circular plate, 26_ first rotating frame, 27_ second support circular plate, 28_ discharge frame, 29_ ball valve, 3_ aeration assembly, 31_ air inlet frame, 32_ aeration frame, 33_ fixed arc plate, 34_ rotating circular frame, 4_ agitating assembly, 41_ oscillating block, 42_ agitating plate, 43_ ring gear, 44_ first straight gear, 5_ illumination assembly, 51_ reflecting frame, 52_ lamp tube, 53_ concave lens, 6_ illumination intensity control assembly, 61_ refracting and transmitting plate, 62_ third support frame, 63_ slotted pushing frame, 64_ first spring, 65_ limiting rod, 66_ limiting circular frame, 67_ second spring, 7_ pushing assembly, 71_ second rotating frame, 72_ second straight gear, 73_ third rotating frame, 74_ third straight gear, 75_ fourth support frame, 76_ beveled ring frame, 761_ column holder, 77_ third spring, 78_ slotted turret, 8_ guard assembly, 81_ guard plate, 82_ fourth turret, 83_ fifth support frame, 84_ sliding slotted plate, 85_ slotted frame.
Detailed Description
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "connected" and "connected" are to be interpreted broadly, e.g., as being fixed or detachable or integrally connected; can be mechanically or electrically connected; may be directly connected or indirectly connected through an intermediate. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Example 1
A bioreactor capable of supplying light based on microalgae density comprises a first support frame 1, a second support frame 21, a support bottom frame 22, a transparent reaction frame 23, a motor 24, a first support circular plate 25, a first rotating frame 26, a second support circular plate 27, a discharge frame 28, a ball valve 29, an aeration component 3 and a stirring component 4, wherein the top of the first support frame 1 is connected with the second support frame 21, the second support frame 21 is connected with the support bottom frame 22, the support bottom frame 22 is connected with the transparent reaction frame 23 through bolts, the transparent reaction frame 23 is made of transparent materials, the transparent reaction frame 23 is communicated with the support bottom frame 22, the first support frame 1 is fixedly provided with the motor 24 for driving, the transparent reaction frame 23 is connected with the first support circular plate 25, the first support circular plate 25 is rotatably connected with the first rotating frame 26, the first rotating frame 26 is rotatably connected with a second supporting circular plate 27, the outer bottom of the supporting bottom frame 22 is connected with a discharging frame 28 for discharging reacted culture solution containing microalgae, the discharging frame 28 is communicated with the supporting bottom frame 22, the discharging frame 28 is rotatably connected with a ball valve 29 for temporarily blocking the discharging frame 28, the supporting bottom frame 22 is provided with an aeration component 3 for timely removing dissolved oxygen generated by photosynthesis of the microalgae, and the transparent reaction frame 23 is provided with a stirring component 4 for improving the photosynthesis efficiency of the microalgae.
Aeration component 3 is including admitting air frame 31, aeration frame 32, fixed arc 33 and rotation circle frame 34, it has frame 31 to admit air to support the switch-on of underframe 22 outer bottom, it has aeration frame 32 to support underframe 22 internal connection, frame 31 of admitting air is used for to the space between underframe 22 and the aeration frame 32 injection air, it has intensive aperture to open on the aeration frame 32, it has six fixed arc 33 to be the hookup of cyclic annular distributing type on the aeration frame 32, fixed arc 33 is fan-shaped structure, the last rotation type of aeration frame 32 is connected with rotation circle frame 34, rotation circle frame 34 closely laminates with fixed arc 33.
The stirring component 4 comprises a vibrating block 41, a stirring plate 42, a gear ring 43 and a first straight gear 44, the inner wall of the transparent reaction frame 23 is connected with the three vibrating blocks 41 in a linear distribution manner, the vibrating blocks 41 are used for enabling the culture solution containing the microalgae in the transparent reaction frame 23 to fluctuate up and down, the bottom of the first rotating frame 26 is fixedly connected with the stirring plate 42 for stirring the culture solution containing the microalgae in the transparent reaction frame 23, the gear ring 43 is connected with the outside of the first rotating frame 26, one end of an output shaft of the motor 24 is connected with the first straight gear 44, and the first straight gear 44 is meshed with the gear ring 43.
The big circular tube of the second supporting circular plate 27 is connected with the culture solution supply device containing microalgae outside, the culture solution containing microalgae is injected into the transparent reaction frame 23 through the second supporting circular plate 27, the air inlet frame 31 is connected with the external air supply device, the air is injected into the space between the supporting bottom frame 22 and the aeration frame 32 through the air inlet frame 31, because the aeration frame 32 is provided with dense small holes, the air in the space between the supporting bottom frame 22 and the aeration frame 32 can be continuously discharged into the transparent reaction frame 23 and generates tiny bubbles, the dissolved oxygen generated by the photosynthesis of microalgae can be timely removed through the tiny bubbles, the gas-liquid exchange in the culture solution is continuously carried out, the unnecessary air is discharged through the small circular tube of the second supporting circular plate 27, the process is aeration, the efficiency of the photosynthesis of microalgae is improved, the manual control motor 24 is started and drives the first straight gear 44 to slowly rotate, the first spur gear 44 drives the gear ring 43 and the upper device thereof to rotate, so that the stirring plate 42 stirs the culture solution containing the microalgae in the transparent reaction frame 23, which is beneficial to aeration, and due to the action of the vibrating block 41, the culture solution containing the microalgae in the transparent reaction frame 23 fluctuates up and down, so that the longitudinal distribution of the culture solution containing the microalgae in the transparent reaction frame 23 is changed, the contact area between the microalgae and external light is increased, the photosynthesis efficiency of the microalgae is further improved, after the reaction is completed, the motor 24 is manually controlled to be closed, and then the ball valve 29 is manually rotated and opened, so that the culture solution containing the microalgae after the reaction in the transparent reaction frame 23 is completed is discharged through the discharging frame 28, and the device can be used again to efficiently culture the microalgae by repeating the above operations.
Example 2
On the basis of embodiment 1, as shown in fig. 8, the device further includes an illumination assembly 5, the illumination assembly 5 for sufficiently illuminating the microalgae inside the transparent reaction frame 23 is disposed on the first rotating frame 26, the illumination assembly 5 includes a reflection frame 51, a lamp tube 52 and a concave lens 53, the reflection frame 51 is symmetrically coupled to the bottom of the first rotating frame 26, the lamp tube 52 for providing additional illumination is coupled to the inside of the reflection frame 51, and the concave lens 53 for refracting light of the lamp tube 52 is symmetrically coupled to the reflection frame 51.
The lamp 52 is started by manually controlling the external control device, the lamp 52 can irradiate the transparent reaction frame 23 and provide illumination for the microalgae in the transparent reaction frame, the reflection frame 51 and the device thereon can slowly rotate around the transparent reaction frame 23 in the process that the first rotating frame 26 and the device thereon slowly rotate, so that the lamp 52 can sufficiently irradiate the microalgae in the transparent reaction frame 23, the light of the lamp 52 can be refracted through the concave lens 53, and meanwhile, the concave lens 53 corresponds to the concave part of the transparent reaction frame 23, so that the light can irradiate deep in the microalgae, the condition that the illumination intensity near the center of the transparent reaction frame 23 is weak is avoided, and the photosynthesis efficiency of the microalgae is further improved.
Example 3
On the basis of embodiment 2, as shown in fig. 9, 10, 11 and 12, the lighting intensity control module 6 is further included, the lighting intensity control module 6 for changing the supply amount of lighting according to the density of microalgae is disposed on the reflection frame 51, the lighting intensity control module 6 includes a refraction transparent plate 61, a third support frame 62, a slotted push frame 63, a first spring 64, a limiting rod 65, a limiting circular frame 66 and a second spring 67, the reflection frame 51 is symmetrically and slidably connected with the refraction transparent plate 61 for weakening the light irradiated from the lamp 52 to the inside of the transparent reaction frame 23, the outer bottom of the reflection frame 51 is connected with the third support frame 62, the third support frame 62 is slidably connected with the slotted push frame 63, the slotted push frame 63 is symmetrically provided with inclined sliding grooves, the slotted push frame 63 is in limiting fit with the refraction transparent plate 61, the first spring 64 is connected between the slotted push frame 63 and the third support frame 62, the second support frame 21 is symmetrically connected with limiting rods 65, a limiting circular frame 66 is connected between the two limiting rods 65 in a sliding mode, the limiting circular frame 66 is of a circular table structure, the limiting circular frame 66 is in contact with the slotting pushing frame 63, and second springs 67 are symmetrically connected between the limiting circular frame 66 and the second support frame 21.
Through the refraction light-transmitting plate 61, the light irradiated from the lamp tube 52 to the inside of the transparent reaction frame 23 can be weakened to adapt to the condition that excessive illumination is not needed in the initial stage of microalgae culture, when the microalgae in the transparent reaction frame 23 reacts to a certain degree, the density of the microalgae can be increased, and illumination needs to be increased, the limiting circular frame 66 is manually pushed to move upwards and extrude the slotting pushing frame 63 to move towards the direction away from each other, the second spring 67 can be compressed accordingly, the first spring 64 can be stretched accordingly, the slotting pushing frame 63 can drive the same pair of refraction light-transmitting plates 61 to move towards the direction away from each other, so that the refraction light-transmitting plates 61 can not refract the light irradiated from the lamp tube 52 to the inside of the transparent reaction frame 23, further, the light of the lamp tube 52 is directly irradiated to the transparent reaction frame 23, and illumination is increased, thereby being beneficial to the culture of the microalgae.
Example 4
On the basis of embodiment 3, as shown in fig. 13, 14 and 15, the air intake device further includes a pushing assembly 7, the pushing assembly 7 is arranged on the air intake frame 31 and used for replacing a human to manually push the limiting circular frame 66 to move upwards, the pushing assembly 7 includes a second rotating rack 71, a second spur gear 72, a third rotating rack 73, a third spur gear 74, a fourth supporting rack 75, a bevel ring frame 76, a column clamping rack 761, a third spring 77 and a slotted rotating rack 78, the second rotating rack 71 is rotatably connected inside the air intake frame 31, the second spur gear 72 is connected to the second rotating rack 71, the second spur gear 72 is located outside the air intake frame 31, the third rotating rack 73 is rotatably connected to the outside of the air intake frame 31, the third rotating rack 73 is connected to the third spur gear 74 through a bolt, the third spur gear 74 is meshed with the second spur gear 72, the fourth supporting rack 75 is connected to the second supporting rack 21, the bevel ring frame 76 is slidably connected to the fourth supporting rack 75, one side of the inclined ring frame 76 is in an inclined surface structure, the inclined ring frame 76 is in contact with the third rotating frame 73, the inclined ring frame 76 is connected with a clamping column frame 761, a third spring 77 is connected between the inclined ring frame 76 and the fourth supporting frame 75, the second supporting frame 21 is rotatably connected with a slotting rotating frame 78, the slotting rotating frame 78 is in an L shape, a straight-line-shaped sliding groove is formed in the slotting rotating frame 78, and the slotting rotating frame 78 is in limit fit with the clamping column frame 761.
In the process that the gas enters the space between the support bottom frame 22 and the aeration frame 32 through the gas inlet frame 31, the second rotating frame 71 and the device thereon rotate and enable the second straight gear 72 to drive the third straight gear 74 and the device thereon to rotate for a circle, then the third rotating frame 73 contacts with the inclined plane of the inclined plane ring frame 76, at the moment, the microalgae in the transparent reaction frame 23 react to a certain degree, the third rotating frame 73 can push the inclined plane ring frame 76 and the device thereon to move towards the direction far away from the gas inlet frame 31, the third spring 77 can be compressed along with the third straight gear, due to the action of the clamping column frame 761, the slotting rotating frame 78 can rotate and push the limiting circular frame 66 to move upwards instead of manually pushing the limiting circular frame 66 to move upwards, the trouble of manual operation is omitted, then the third rotating frame 73 can be separated from the inclined plane ring frame 76, the inclined plane ring frame 76 and the device thereon can be reset reversely, the purpose of changing the supply amount of light according to the density of microalgae is achieved.
Example 5
On the basis of the embodiment 4, as shown in fig. 16 and 17, a protective assembly 8 is further included, a protection component 8 for preventing aeration frame 32 from being blockked up is located on first support frame 1, protection component 8 is including the guard plate 81, fourth rotating turret 82, fifth support frame 83, slip fluting board 84 and fluting frame 85, it has six guard plates 81 to be the hookup of cyclic annular distributing type on the circle of rotation frame 34, guard plate 81 is fan-shaped structure, it has fourth rotating turret 82 to slide formula on the support underframe 22, fourth rotating turret 82 couples with circle of rotation frame 34, first support frame 1 top hookup has fifth support frame 83, it has slip fluting board 84 to slide formula on the fifth support frame 83, it has the slant spout to open on the slip fluting board 84, slip fluting board 84 and fourth rotating turret 82 limit fit, it has fluting frame 85 to link on the slip fluting board 84, fluting frame 85 is the L type, fluting frame 85 and the limit fit of ball valve 29.
Before discharging the culture solution containing microalgae in the transparent reaction frame 23, the air inlet frame 31 stops conveying air, and at the moment, the air inlet frame 32 is easily blocked by the microalgae in the air inlet frame 31, so that the air inlet frame 31 stops conveying air, and meanwhile, the fourth rotating frame 82 and the upper device thereof are manually pulled to move along the sliding groove in the supporting bottom frame 22, so that the protection plate 81 and the fixed arc plate 33 cover the aeration frame 32 together, the aeration frame 32 is prevented from being blocked, meanwhile, the fourth rotating frame 82 drives the sliding slotted plate 84 and the upper device thereof to move downwards, the slotted frame 85 drives the ball valve 29 to rotate and open, the ball valve 29 is manually rotated to replace a human, and the culture solution containing microalgae in the transparent reaction frame 23 is conveniently discharged.
The embodiments of the present invention have been presented for purposes of illustration and description, and are not intended to be exhaustive or limited to the invention in the form disclosed. Many modifications and variations will be apparent to those of ordinary skill in the art. The embodiment was chosen and described in order to best explain the principles of the invention and the practical application, and to enable others of ordinary skill in the art to understand the invention for various embodiments with various modifications as are suited to the particular use contemplated.
Claims (9)
1. The utility model provides a can carry out bioreactor that illumination was supplied with based on little algae density which characterized in that, including first support frame, second support frame, support underframe, transparent reaction frame, motor, first support plectane, first revolving rack, second support plectane, ejection of compact frame, ball valve, aeration subassembly and stirring subassembly:
the top of the first support frame is connected with a second support frame;
the second support frame is connected with the support bottom frame;
the transparent reaction frame is connected to the supporting bottom frame through bolts and communicated with the supporting bottom frame, and the transparent reaction frame is cylindrical;
the motor is fixedly arranged on the first support frame and used for driving the equipment to operate;
a first supporting circular plate is connected to the transparent reaction frame,
the first rotating frame is rotatably connected to the first supporting circular plate,
a second supporting circular plate is rotatably connected to the first rotating frame,
the discharging frame is connected to the outer bottom of the supporting bottom frame and is communicated with the supporting bottom frame, and the discharging frame is used for discharging the well-reacted culture solution containing the microalgae;
the ball valve is rotatably connected to the discharging frame and used for temporarily blocking the discharging frame;
the aeration component is arranged on the supporting bottom frame and used for removing dissolved oxygen generated by photosynthesis of the microalgae in time;
the stirring component is arranged on the transparent reaction frame and used for improving the photosynthesis efficiency of the microalgae.
2. The bioreactor of claim 1, wherein the outer wall of the transparent reaction frame is symmetrically concave to increase the amount of light that can enter the transparent reaction frame, thereby reducing the dead zone of light irradiation of microalgae.
3. The bioreactor capable of supplying light based on microalgae density as claimed in claim 1, wherein the aeration assembly comprises an air inlet frame, an aeration frame, a fixed arc plate and a rotating circular frame, the air inlet frame is connected to the outer bottom of the support bottom frame, the aeration frame is connected to the inside of the support bottom frame, six fixed arc plates are connected to the aeration frame in an annular distribution manner, the rotating circular frame is rotatably connected to the aeration frame, and the rotating circular frame is tightly attached to the fixed arc plates.
4. The bioreactor capable of providing light based on microalgae density as claimed in claim 3, wherein the stirring assembly comprises an oscillating block, a stirring plate, a gear ring and a first straight gear, the three oscillating blocks are connected to the inner wall of the transparent reaction frame in a linear distribution manner, the stirring plate is fixedly connected to the bottom of the first rotating frame, the gear ring is connected to the outside of the first rotating frame, the first straight gear is connected to one end of the output shaft of the motor, and the first straight gear is meshed with the gear ring.
5. The bioreactor capable of providing illumination based on microalgae density as claimed in claim 4, further comprising an illumination assembly disposed on the first rotating frame, wherein the illumination assembly comprises a reflecting frame, a lamp tube and a concave lens, the bottom of the first rotating frame is symmetrically connected with the reflecting frame, the lamp tube is connected to the inside of the reflecting frame, and the concave lens is symmetrically connected to the reflecting frame.
6. The bioreactor according to claim 5, wherein the light supply is based on microalgae density, the light intensity control assembly is arranged on the reflection frame and comprises a refraction light-transmitting plate, a third support frame, a slotting push frame, a first spring, a limiting rod, a limiting round frame and a second spring, the refraction light-transmitting plate is symmetrically and slidably connected to the reflection frame, the third support frame is connected to the outer bottom of the reflection frame, the slotting push frame is slidably connected to the third support frame and is in limiting fit with the refraction light-transmitting plate, the first spring is connected between the slotting push frame and the third support frame, the limiting rod is symmetrically connected to the second support frame, the limiting round frame is connected between the two limiting rods in a sliding mode, the limiting round frame is in contact with the slotting push frame, and the second spring is symmetrically connected between the limiting round frame and the second support frame.
7. The bioreactor of claim 6, wherein the refractive transparent plate has a plurality of protrusions on its surface for reflecting part of light and reducing the amount of light transmission, so as to weaken the light irradiated on the transparent reaction frame.
8. The bioreactor capable of providing illumination based on microalgae density as claimed in claim 6, further comprising a pushing assembly disposed on the air inlet frame, wherein the pushing assembly comprises a second rotating rack, a second spur gear, a third rotating rack, a third spur gear, a fourth supporting rack, a bevel ring frame, a column clamping rack, a third spring and a slotted rotating rack, the second rotating rack is rotatably connected to the inside of the air inlet frame, the second rotating rack is connected to the second rotating rack, the second spur gear is located outside the air inlet frame, the third rotating rack is rotatably connected to the outside of the air inlet frame, the third rotating rack is connected to the third rotating rack through a bolt, the third spur gear is meshed with the second spur gear, the second supporting rack is connected to the fourth supporting rack, the bevel ring frame is slidably connected to the fourth supporting rack, the bevel ring frame is in contact with the third rotating rack, the inclined ring frame is connected with a clamping column frame, a third spring is connected between the inclined ring frame and the fourth support frame, the second support frame is rotatably connected with a slotting rotating frame, and the slotting rotating frame is in limit fit with the clamping column frame.
9. The bioreactor capable of providing illumination based on microalgae density as claimed in claim 8, further comprising a protection assembly, wherein the protection assembly is disposed on the first support frame, the protection assembly comprises a protection plate, a fourth rotating frame, a fifth support frame, a sliding groove plate and a groove opening frame, six protection plates are connected to the rotating circular frame in an annular distribution manner, the fourth rotating frame is connected to the supporting bottom frame in a sliding manner, the fourth rotating frame is connected to the rotating circular frame, the fifth support frame is connected to the top of the first support frame, the sliding groove plate is connected to the fifth support frame in a sliding manner, the sliding groove plate is in limit fit with the fourth rotating frame, the groove opening frame is connected to the sliding groove plate, and the groove opening frame is in limit fit with the ball valve.
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| CN202111386396.4A CN114106979A (en) | 2021-11-22 | 2021-11-22 | Bioreactor capable of supplying illumination based on microalgae density |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115672010A (en) * | 2022-10-31 | 2023-02-03 | 原初科技（北京）有限公司 | Microalgae carbon sequestration device and use method thereof |
| CN115960697A (en) * | 2022-12-19 | 2023-04-14 | 原初科技（北京）有限公司 | Carbon fixing device based on solar energy and using method thereof |
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2021
- 2021-11-22 CN CN202111386396.4A patent/CN114106979A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115672010A (en) * | 2022-10-31 | 2023-02-03 | 原初科技（北京）有限公司 | Microalgae carbon sequestration device and use method thereof |
| CN115672010B (en) * | 2022-10-31 | 2023-04-28 | 原初科技（北京）有限公司 | Microalgae carbon fixing device and application method thereof |
| CN115960697A (en) * | 2022-12-19 | 2023-04-14 | 原初科技（北京）有限公司 | Carbon fixing device based on solar energy and using method thereof |
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