CN114081013A - Method for re-breeding African swine fever patients - Google Patents
Method for re-breeding African swine fever patients Download PDFInfo
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- CN114081013A CN114081013A CN202111385020.1A CN202111385020A CN114081013A CN 114081013 A CN114081013 A CN 114081013A CN 202111385020 A CN202111385020 A CN 202111385020A CN 114081013 A CN114081013 A CN 114081013A
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- 238000000034 method Methods 0.000 title claims abstract description 37
- 208000007407 African swine fever Diseases 0.000 title claims abstract description 24
- 238000009395 breeding Methods 0.000 title claims abstract description 17
- 241000282887 Suidae Species 0.000 claims abstract description 34
- 241000282898 Sus scrofa Species 0.000 claims abstract description 27
- 238000001514 detection method Methods 0.000 claims abstract description 23
- 238000005202 decontamination Methods 0.000 claims abstract description 16
- 230000003588 decontaminative effect Effects 0.000 claims abstract description 16
- 238000002955 isolation Methods 0.000 claims abstract description 7
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 42
- 238000004140 cleaning Methods 0.000 claims description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- 235000011121 sodium hydroxide Nutrition 0.000 claims description 14
- 230000000249 desinfective effect Effects 0.000 claims description 9
- 238000002791 soaking Methods 0.000 claims description 9
- 241000191967 Staphylococcus aureus Species 0.000 claims description 6
- 230000001488 breeding effect Effects 0.000 claims description 6
- 239000002689 soil Substances 0.000 claims description 6
- 238000011156 evaluation Methods 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 5
- 238000010422 painting Methods 0.000 claims description 5
- 238000007789 sealing Methods 0.000 claims description 5
- 238000004519 manufacturing process Methods 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 239000002023 wood Substances 0.000 claims description 4
- 239000008280 blood Substances 0.000 claims description 3
- 210000004369 blood Anatomy 0.000 claims description 3
- 239000004568 cement Substances 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 239000002131 composite material Substances 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 3
- 230000002550 fecal effect Effects 0.000 claims description 3
- 239000012530 fluid Substances 0.000 claims description 3
- 239000002316 fumigant Substances 0.000 claims description 3
- 238000003958 fumigation Methods 0.000 claims description 3
- 239000001963 growth medium Substances 0.000 claims description 3
- ZOCLAPYLSUCOGI-UHFFFAOYSA-M potassium hydrosulfide Chemical compound [SH-].[K+] ZOCLAPYLSUCOGI-UHFFFAOYSA-M 0.000 claims description 3
- 210000003296 saliva Anatomy 0.000 claims description 3
- 239000010865 sewage Substances 0.000 claims description 3
- 239000000779 smoke Substances 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 230000003442 weekly effect Effects 0.000 claims description 3
- 230000003203 everyday effect Effects 0.000 claims description 2
- 239000004744 fabric Substances 0.000 claims description 2
- 238000012423 maintenance Methods 0.000 claims description 2
- 238000012546 transfer Methods 0.000 claims description 2
- 206010037660 Pyrexia Diseases 0.000 abstract description 4
- 230000004083 survival effect Effects 0.000 abstract description 4
- 230000008569 process Effects 0.000 description 7
- 241000701386 African swine fever virus Species 0.000 description 5
- 201000010099 disease Diseases 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 238000005406 washing Methods 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 4
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- 238000002360 preparation method Methods 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 2
- 230000001154 acute effect Effects 0.000 description 2
- 239000000460 chlorine Substances 0.000 description 2
- 229910052801 chlorine Inorganic materials 0.000 description 2
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- 230000036541 health Effects 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 208000035473 Communicable disease Diseases 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 206010011703 Cyanosis Diseases 0.000 description 1
- 208000000059 Dyspnea Diseases 0.000 description 1
- 206010013975 Dyspnoeas Diseases 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Apparatus For Disinfection Or Sterilisation (AREA)
Abstract
The invention belongs to the technical field of raising after swine fever, and particularly discloses a method for re-raising after African swine fever, which comprises the following steps: step 1, evaluating before re-culture, determining a re-culture mode and upgrading a biological safety hardware facility; step 2, after the re-breeding is determined, decontamination is carried out on the pig farm; step 3, detection is carried out after decontamination, and when all detections are negative, the detection is qualified and can be used for re-cultivation; step 4, trial raising, detecting and maintaining sentinel pigs; and 5, adopting a special isolation shed for feeding after successfully trying to raise the sentinel pigs in the step 4, and introducing the binary sows. By adopting the scheme of the invention, the survival rate of the live pigs after African swine fever is improved.
Description
Technical Field
The invention belongs to the technical field of raising after swine fever, and particularly relates to a method for re-raising after African swine fever.
Background
African Swine Fever (ASF) is an acute, hemorrhagic and virulent infectious disease caused by African Swine Fever Virus (ASFV) infecting domestic pigs and various wild pigs (such as African wild pigs and European wild pigs). The world animal health Organization (OIE) classifies the animal epidemic disease as a legal report animal epidemic disease, and the disease is also a type of animal epidemic disease which is mainly prevented in China.
African swine fever is characterized by short disease process, high death rate of acute infection of up to 100 percent, clinical manifestations of fever (up to 40-42 ℃), fast heartbeat, dyspnea, partial cough, serous or mucoid purulent secretion of eyes and nose, cyanosis of skin, obvious bleeding of lymph nodes, kidney and gastrointestinal mucosa, similar clinical symptoms of African swine fever to those of swine fever, and accurate diagnosis only by means of laboratory monitoring.
After the epidemic situation of the African swine fever occurs in a pig farm, the biological safety loophole of the pig farm is checked, the geographic environment, the breeding density, the biological case pressure, the existing biological safety measures of the pig farm and the like around the pig farm are evaluated, and after the re-breeding is determined to be needed, a set of mature re-breeding method is needed to ensure the success rate of the re-breeding.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a method for improving the survival rate of the live pigs after African swine fever.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for recultivation of African swine fever comprises the following steps:
step 1, evaluating before re-culture, determining a re-culture mode and upgrading a biological safety hardware facility;
step 2, after the re-breeding is determined, decontamination is carried out on the pig farm;
step 3, detection is carried out after decontamination, and when all detection results are negative, the detection results are qualified, and recultivation can be carried out;
step 4, trial raising, detecting and maintaining sentinel pigs;
and 5, adopting a special isolation shed for feeding after successfully trying to raise the sentinel pigs in the step 4, and introducing the binary sows.
Further, in the step 1, the evaluation before re-breeding comprises the geographical environment around the pig farm, the breeding density, the biological case pressure and the existing biological safety measures of the pig farm.
Further, in the step 1, the biosafety hardware facilities comprise a transfer pig platform, a material fumigation chamber, a PCR detector and a high-pressure hot water cleaning machine.
Further, in the step 2, the step of decontamination includes:
s1: burning the wood material-removed articles and equipment; if the pig farm has exposed soil, replacing surface soil and hardening cement;
s2: carrying out harmless treatment on the whole fecal sewage, and cleaning and disinfecting;
s3: cleaning a waterline: all water drinkers and joints are dismounted, and the mixture is placed in a caustic soda pool to be soaked for not less than 2 hours;
s4: and (3) soaking and cleaning the removed objects: all the articles in the pigsty are detached, and the pigsty is cleaned and then placed in a caustic soda pool to be soaked for not less than 2 hours;
s5: after cleaning, sterilizing the whole field once every day by using 2% hot and alkaline water for one week;
s6: sealing the pigsty, fumigating with a smoke agent for 24h, and fumigating again after one week;
s7: removing all articles in each room of a living area, an office area and a restaurant, cleaning, and painting wall surfaces; after the cleaning and painting are finished, sterilizing the fabric by using composite potassium hydrogen sulfide once a day for one week; then fumigating with fumigant, and sealing for at least 24 hr.
Further, the detection in the step 3 is carried out by adopting a traditional method for culturing staphylococcus aureus by using a culture medium, if staphylococcus aureus is detected, the piggery is unqualified in decontamination, and the method provided in the step 2 needs to be used again for decontamination of the piggery.
Further, the detection objects in the step 3 include transportation vehicles, surrounding environment, pigsty in production area, offices, pigsty and kitchens.
Further, the sentinel pigs are cultured for 1-2 months in the step 4, and PCR detection is required to be carried out on the sentinel pigs before introduction; nasal fluid, saliva and blood samples of sentinel pigs were collected weekly and submitted for examination.
Further, in the step 5, the sentinel pigs which are successfully tried to be raised are fed in an isolation shed.
Compared with the prior art, the invention has the following beneficial effects:
in the method for recultivation of African swine fever, provided by the invention, large environment assessment is comprehensively considered (for example, whether sites meet PIC thousand-point assessment requirements or not and whether epidemic situation exists around the recent time or not); evaluating main risk points inside and outside a field; checking for defects and mending leakage (upgrading and transforming facilities and equipment, and perfecting management system and flow); comprehensively decontaminating (disinfecting, cleaning, disinfecting, fumigating and drying); evaluation after washout (comprehensive sampling and professional testing); feeding sentinel pigs and continuously and dynamically monitoring; health examination and pathogen monitoring of pigs to be introduced; the whole process of preparing the pigs carefully before transportation and monitoring the pig transportation process is carried out; thus, the survival rate of the re-culture can be ensured.
Detailed Description
The present invention will be described in further detail below, and specific embodiments thereof will be described.
The embodiment provides a method for recultivation after African swine fever, which comprises the following steps:
step 1, evaluation preparation before re-cultivation, wherein the evaluation preparation comprises the following steps:
s1: and (4) checking biological safety holes, evaluating the geographical environment around the pig farm, the breeding density, the biological case pressure, the existing biological safety measures of the pig farm and the like, and determining whether to carry out re-breeding.
S2: determining a re-cultivation mode: selecting a set of pigs for production or secondary feeding (namely, separately feeding sows and fattening pigs).
S3: upgrading the biosafety hardware facilities: if the pigsty is completely closed, a transshipment pig platform, a material fumigation chamber, a PCR detector, a high-pressure hot water washing machine (a farm decontamination station) and the like are prepared, wherein the fluorescence PCR detector and the high-pressure hot water washing machine are necessary products for preventing and controlling African swine fever in the pigsty.
In addition, the high-pressure hot water cleaning machine needs to be matched with a foam cleaning agent and a high-quality disinfectant and is matched with a vehicle for use, so that a better effect can be achieved.
Step 2, after the re-cultivation is determined, decontamination is carried out, and the decontamination step comprises the following steps:
s1: burning the wood material-removed articles and equipment; if the pig farm has exposed soil, the surface soil is replaced and cement hardening is carried out comprehensively.
S2: carrying out harmless treatment on the fecal sewage in the whole field: and (4) emptying the liquid dung in the liquid dung pool and the pit, performing harmless treatment (such as fermentation for 45 days), and thoroughly cleaning and disinfecting to a new degree.
S3: cleaning a waterline: all the drinkers, joints and the like are dismounted, and the mixture is placed in a caustic soda pool for soaking for 2 hours. The waterline surface is thoroughly cleaned in the pigsty cleaning process and is qualified through inspection. Turning off the main switch, adding a chlorine preparation disinfectant containing 3% of available chlorine into a water tank or a reservoir, and soaking for 2 hours; after the drinkers are refilled, the farthest drinker in the pig farm is opened, so that the disinfectant in the reservoir fills the whole pipeline and is soaked for more than 2 hours. After soaking, the sterilized water in the water pipeline is discharged, and then clear water is injected.
S4: and (3) soaking and cleaning the removed objects:
building a caustic soda pool with a proper size according to the condition of a pig farm, wherein a larger caustic soda pool is needed if all location fences and obstetric tables are soaked; for example, the area of the caustic soda pool constructed in the field is 4.5m2And water was poured to a depth of 0.6m to 40cm, and 36kg of caustic soda (about 2% sodium hydroxide solution) was poured in.
And all articles in the pigsty need to be detached, and the pigsty is thoroughly cleaned and then placed in a caustic soda pool for soaking for 2 hours. In case of more articles, the method of batch soaking is adopted, and 10kg of caustic soda is added before each soaking. 5 batches of disinfectant are replaced once, and all articles soaked by caustic soda need to be cleaned and dried by clear water.
S5: after washing, the whole field was sterilized with 2% hot and alkaline water once a day for one week. Spraying water on the ground, and keeping for 30min without drying.
S6: sealing the pigsty, fumigating with smoke agent for 24 hr, and fumigating again after one week.
S7: disinfecting a life area and an office area of workers: all articles in each room such as living area, office area, restaurant and the like are removed for thorough cleaning. And (5) painting the wall surface. After the cleaning and brushing are finished, the mixture is disinfected by composite potassium hydrogen sulfide once a day for one week.
Then fumigate with fumigant (according to the dosage of the instruction) and seal for 24 h.
Step 3, detection after decontamination:
1) and after the piggery is washed and disinfected in the step 2, drying the piggery, and inspecting the piggery by adopting a traditional method for culturing staphylococcus aureus by using a culture medium, wherein if the staphylococcus aureus is detected, the piggery is unqualified in washing and disinfecting, and the method provided in the step 2 needs to be used again for washing and disinfecting the piggery.
2) And carrying out decontamination detection on the transported vehicle by adopting ATP detection (the result is obtained in 10-15 s).
3) ASFV can be detected by fluorescence PCR through multipoint sampling (surrounding environment, material personnel and vehicles, pigsty in production area, office, pigsty, kitchen, etc.), and when the detection is negative, the ASFV is qualified and can be reared.
Step 4, trial breeding, detection and maintenance of sentinel pigs:
1) and the sentinel pigs with the sample of 1-2 months: the number of sentinel pigs is 20% as the best, 2-3 sentinel pigs are used in each circle, each sentinel pig is a negative pig with about 25kg, PCR detection is needed before the sentinel pigs are introduced, and emergency measures are needed after rechecking when the content of the African swine fever virus in the swinery is more than or equal to 100 TCID 50.
2) And weekly sampling and inspection: nasal fluid, saliva and blood samples of sentinel pigs are collected for inspection and need to be sent to an MA (mass authentication) detection mechanism.
Step 5, introduction and isolation:
after the sentinel pigs are successfully raised in the test raising step 4, the sentinel pigs are raised in a special isolation shed and can be combined with GDU into a whole; the source of the binary sow is reliable, and the negative PCR detection is ensured; on the principle of purchasing nearby, the provincial boundary is preferably not crossed; and currently, cross-provincial introduction is carried out by reporting the approval of the provincial hall.
The introduction transportation process needs to execute three principles: the driver does not get off the bus without stopping the bus or entering the service area. The seeds are introduced back to the field and are detected again after half a month. In addition, special people are required for feeding, and feeding tools need to be separated.
By adopting the steps, the survival rate of the live pigs after the African swine fever is ensured.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Finally, the above embodiments are only for illustrating the technical solutions of the present invention and not for limiting, although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, and all of them should be covered in the claims of the present invention.
Claims (8)
1. A method for recultivation of African swine fever is characterized in that: the method comprises the following steps:
step 1, evaluating before re-culture, determining a re-culture mode and upgrading a biological safety hardware facility;
step 2, after the re-breeding is determined, decontamination is carried out on the pig farm;
step 3, detection is carried out after decontamination, and when all detections are negative, the detection is qualified and can be used for re-cultivation;
step 4, trial breeding, detection and maintenance of sentinel pigs;
and 5, adopting a special isolation shed for feeding after successfully trying to raise the sentinel pigs in the step 4, and introducing the binary sows.
2. The method for the afterward recuperation of African swine fever according to claim 1, wherein: in the step 1, the evaluation before the re-breeding comprises the geographical environment around the pig farm, the breeding density, the biological case pressure and the existing biological safety measures of the pig farm.
3. The method for the recuperation of African swine fever after, according to claim 2, characterized in that: in the step 1, the biosafety hardware facilities comprise a transfer pig loading platform, a material fumigation chamber, a PCR detector and a high-pressure hot water cleaning machine.
4. The method for the recuperation of African swine fever after, according to claim 3, wherein the method comprises the following steps: in the step 2, the decontamination step comprises:
s1: removing the wood material, and burning the wood material-removed article and equipment; if the pig farm has exposed soil, replacing surface soil and hardening cement;
s2: carrying out harmless treatment on the whole fecal sewage, and cleaning and disinfecting;
s3: cleaning a waterline: all water drinkers and joints are dismounted, and the mixture is placed in a caustic soda pool to be soaked for not less than 2 hours;
s4: and (3) soaking and cleaning the removed objects: all the articles in the pigsty are detached, and the pigsty is cleaned and then placed in a caustic soda pool to be soaked for not less than 2 hours;
s5: after cleaning, sterilizing the whole field once with 2% caustic soda water every day for one week;
s6: sealing the pigsty, fumigating with a smoke agent for 24h, and fumigating again after one week;
s7: removing all articles in each room of a living area, an office area and a restaurant, cleaning, and painting wall surfaces; after cleaning and painting are finished, disinfecting the fabric with composite potassium hydrogen sulfide once a day for one week; then fumigating with fumigant, and sealing for at least 24 hr.
5. The method for the recuperation of African swine fever after, according to claim 4, wherein the method comprises the following steps: and 3, detecting in the step 3 by adopting a traditional method for culturing staphylococcus aureus by using a culture medium, and if staphylococcus aureus is detected, indicating that the piggery is unqualified in decontamination, and decontaminating the piggery by using the method provided in the step 2 again.
6. The method for the recuperation of African swine fever after, according to claim 5, wherein the method comprises the following steps: the detection objects in the step 3 comprise transportation vehicles, surrounding environment, pigsty in a production area, offices, pigsties and kitchens.
7. The method for the recuperation of African swine fever after, according to claim 6, wherein the method comprises the following steps: in the step 4, the sentinel pigs are cultured for 1-2 months in a trial mode, and PCR detection is needed to be carried out on the sentinel pigs before introduction; nasal fluid, saliva and blood samples of sentinel pigs were collected weekly and submitted for examination.
8. The method for the recuperation of African swine fever after, according to claim 7, wherein: in the step 5, the sentinel pigs which are successfully tried to be raised are fed in an isolation shed.
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| CN202111385020.1A CN114081013A (en) | 2021-11-22 | 2021-11-22 | Method for re-breeding African swine fever patients |
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| CN202111385020.1A CN114081013A (en) | 2021-11-22 | 2021-11-22 | Method for re-breeding African swine fever patients |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110537492A (en) * | 2019-09-10 | 2019-12-06 | 大鸿农牧科技研究院重庆有限公司 | Pig farm for preventing and controlling African swine fever and isolation disinfection method |
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2021
- 2021-11-22 CN CN202111385020.1A patent/CN114081013A/en active Pending
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|---|---|---|---|---|
| CN110537492A (en) * | 2019-09-10 | 2019-12-06 | 大鸿农牧科技研究院重庆有限公司 | Pig farm for preventing and controlling African swine fever and isolation disinfection method |
| CN211430475U (en) * | 2019-09-10 | 2020-09-08 | 大鸿农牧科技研究院重庆有限公司 | Pig farm for preventing and controlling African swine fever |
| CN111218527A (en) * | 2020-03-10 | 2020-06-02 | 广州赛百纯生物科技有限公司 | Environment sample African swine fever virus detection kit and detection method |
| CN112403997A (en) * | 2020-10-30 | 2021-02-26 | 康德士环保科技(中山)有限公司 | Cleaning and disinfecting method for preventing and controlling African swine fever |
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| Title |
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