CN114073279A - Method for improving feed fermentation effect by controlling fermentation process of multiple strains - Google Patents
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Abstract
A method for improving the fermentation effect of feed by controlling the fermentation process of multiple strains comprises the technological route of 'experiment and data acquisition-production data input-organization production by using a multi-stage fermentation system-discharge and post-treatment', provides a production-oriented industrialization scheme aiming at staged composite strain fermentation cultivation, can realize the intelligent arrangement of staged composite strain fermentation cultivation by determining complete set of operation data and parameters in the experiment and data acquisition stage and programming the operation data and parameters for the automatic multi-stage fermentation system to call and organize production, can adjust the experiment data and parameters on line, selects the staged mixed fermentation on-line carrying-out mode according to the occupation and idle conditions of required strains and various chambers of equipment, and can almost meet all the common composite fermentation requirements in production by one set of production system, the process route of the invention solves the production problem in the staged fermentation culture of the composite strain.
Description
Technical Field
The invention relates to the technical field of preparation of fermented feeds, in particular to a method for improving the fermentation effect of feeds by controlling the fermentation process of multiple strains.
Background
In the preparation of fermented feed, the most common method is multi-strain mixed fermentation, because single-strain fermentation uses single microorganism, the enzyme systems participating in biochemical reaction are few, and most important biochemical processes cannot be completed well or can only be performed weakly, while different microorganisms in the mixed fermentation process have different metabolic pathways, and the multiple microorganisms have synergistic effects, so that the yield and the viable count can be improved by advantage complementation, and the feed fermentation effect is further improved.
At present, the preparation process of multi-strain mixed fermentation feed for production mainly adopts the way of directly mixing materials to be fermented after single-strain fermentation cultivation as a main route, and is developed mainly aiming at respective environmental conditions of single-strain fermentation cultivation and action conditions of mixed flora in the feed fermentation stage in research, for example, the composite probiotic culture and the preparation method thereof disclosed in the Chinese patent application 201610138323.6, after seed fermentation preparation of saccharomyces cerevisiae liquid, bacillus subtilis liquid and enterococcus faecalis liquid is respectively carried out, the prepared three types of liquid are directly mixed with raw materials to be fermented and water and are filled into a fermentation cylinder for sealed fermentation, and fermentation is carried out for 120-150h at 25-28 ℃, the operation flow of the process is simple and easy to industrialize, and optimized culture medium and temperature are easily provided according to different strains in the strain fermentation cultivation stage, The method is characterized in that the method comprises the steps of carrying out mixed fermentation cultivation on a plurality of strains obtained by enlarged cultivation, and then mixing materials to be fermented for fermentation, wherein the process operation also has the advantages of simple process and easy industrialization.
In view of the above disadvantages, the present applicant has already developed some research and production verification for staged fermentation and cultivation of complex strains in the industry, but no industrial solution for production is implemented at present.
Disclosure of Invention
In order to solve the problems, the invention provides a method for improving the fermentation effect of the feed by controlling the fermentation process of multiple strains, and provides a production-oriented industrial scheme aiming at staged composite strain fermentation and cultivation.
The purpose of the invention is realized by the following technical scheme.
A method for improving the fermentation effect of feed by controlling the fermentation process of multiple strains comprises the following process routes:
(1) experiment and data acquisition
Selecting strains commonly used in production, wherein at least three strains are marked as a first strain, a second strain and a third strain respectively;
the respective optimal fermentation and cultivation conditions of each strain are determined through experiments, the fermentation and cultivation conditions at least comprise fermentation temperature, fermentation duration conditions and culture medium conditions, and the following are recorded respectively: i the optimum fermentation temperature condition of the first strain is T1The optimal fermentation time period condition is t1The optimal culture medium condition is S1(ii) a II the optimum fermentation temperature condition of the second strain is T2The optimal fermentation time period condition is t2The optimal culture medium condition is S2(ii) a III the optimal fermentation temperature condition of the third strain is T3The optimal fermentation time period condition is t3The optimal culture medium condition is S3；
The method comprises the following steps of determining optimal conditions for staged composite strain fermentation cultivation by experiments, wherein the staged composite strain fermentation cultivation at least comprises a first fermentation cultivation stage and a second fermentation cultivation stage, the first fermentation cultivation stage is a single strain fermentation cultivation stage and at least comprises a first fermentation cultivation stage of a first strain and a first fermentation cultivation stage of a second strain, the second fermentation cultivation stage is a mixed fermentation cultivation stage of more than two strains and at least comprises a mixed strain of the first strain and the second strainThe optimal conditions determined by experiments in the mixed fermentation cultivation stage at least comprise the following conditions: i optimal fermentation time length t of first fermentation cultivation stage of first strain1' and the optimal fermentation time t of the first fermentation cultivation stage of the second species2', wherein, t1′＜t1，t2′＜t2(ii) a II optimum fermentation temperature condition T of the second fermentation cultivation stage of the mixed strain of the first strain and the second straineAnd an optimum fermentation time te；
(2) Production data entry
Recording all or part of the optimal conditions determined in the experiment of the step (1) into a production management system and/or a production control system, wherein the production management system can provide online original data for the production of the fermented feed, and the production control system can directly provide an executable control program for the production of the fermented feed;
(3) organizing production using a multi-stage fermentation system
Producing fermented feed by using an automatic fermented feed production system according to the production data entered in the step (2), wherein:
the automatic fermented feed production system comprises at least two stages of strain fermentation and cultivation systems and at least one stage of raw material fermentation system, wherein the first stage of strain fermentation and cultivation system comprises at least two first-stage fermentation bins which are respectively a first-stage fermentation bin and a second-stage fermentation bin, the second-stage strain fermentation and cultivation system comprises at least one first second-stage fermentation bin, the first-stage raw material fermentation system comprises at least one first-stage raw material fermentation bin, during production, fermentation and cultivation are carried out on a first strain in the first-stage fermentation bin according to the determined optimal fermentation temperature condition and optimal culture medium condition of the first strain, and the cultivation duration is the optimal fermentation duration t of the first fermentation and cultivation stage of the first strain determined in the step (1)1', the optimal fermentation temperature condition and the optimal culture medium condition are respectively the optimal fermentation temperature condition T of the first strain determined in the step (1)1And optimal Medium Condition S1Or according to the optimal fermentation temperature condition T of the first strain determined in the step (1)1And optimal Medium Condition S1Performing manual intervention on the adjusted new data; fermenting and culturing the second strain in the second primary fermentation bin and/or the first secondary fermentation bin according to the determined optimal fermentation temperature condition and optimal culture medium condition of the second strain, wherein the culture time is the optimal fermentation time t of the first fermentation culture stage of the second strain determined in the step (1)2', the optimal fermentation temperature condition and the optimal culture medium condition are respectively the optimal fermentation temperature condition T of the second strain determined in the step (1)2And optimal Medium Condition S2Or according to the optimal fermentation temperature condition T of the first strain determined in the step (1)2And optimal Medium Condition S2Performing manual intervention on the adjusted new data;
setting the initial fermentation and cultivation time of the first strain and the second strain to ensure that the time length t of the first fermentation and cultivation stage of the first strain1Period of and length of first fermentation incubation period t of the second species2' then, the first and second strains completing the first fermentation incubation stage are mixed by operating the control valve for a time period teWhen the second strain is fermented and cultivated in the first fermentation and cultivation stage in the first primary fermentation bin, the first strain in the first primary fermentation bin and the second strain in the second primary fermentation bin flow into the first secondary fermentation bin and are mixed in the first secondary fermentation bin by opening a control valve on a path leading from the first primary fermentation bin to the second primary fermentation bin, wherein the duration is teThe second fermentation cultivation stage of (1); when the second strain is fermented and cultivated in the first secondary fermentation bin in the first fermentation cultivation stage, the first strain in the first primary fermentation bin and the second strain originally existing in the first secondary fermentation bin are mixed by opening a control valve on a path leading from the first primary fermentation bin to the first secondary fermentation bin, and the first strain and the second strain are fermented and cultivated in the first secondary fermentation bin for the time period teThe second fermentation cultivation stage of (1); in the second fermentation incubation period, the fermentation temperature conditions are selected to be the most favorable conditions determined in step (1)Optimum fermentation temperature condition Te；
The above time period is teAfter the second fermentation cultivation stage, directly introducing the mixed strain obtained in the first secondary fermentation bin into a first primary raw material fermentation bin connected with the first secondary fermentation bin, and performing mixed fermentation with fermentation raw materials to prepare fermented feed, or introducing the mixed strain obtained in the first secondary fermentation bin into the first primary raw material fermentation bin after mixing with one or more other strains which are already fermented and cultivated in a staged manner and performing a new mixed fermentation cultivation stage;
(4) discharge and after-treatment
After the fermentation of the raw materials in the first primary raw material fermentation bin is finished, the raw materials are released by operating a discharge control valve of the first primary raw material fermentation bin and are subjected to subsequent treatment.
Preferably, in the method for improving the fermentation effect of the feedstuff by controlling the fermentation process of the plurality of strains, the staged fermentation culture of the composite strain in the step (1) further comprises at least one third fermentation culture stage, and in the third fermentation culture stage, the mixed strain obtained by the culture in the second fermentation culture stage is mixed with one or more other strains which are not fermented or have been only subjected to the stage fermentation culture.
Preferably, in the method for improving the fermentation effect of the feed by controlling the fermentation process of a plurality of strains, the other one or more strains which are not cultured by fermentation or are cultured by only staged fermentation include the third strain in the step (1).
Preferably, in the method for improving the fermentation effect of the feed by controlling the fermentation process of multiple strains, when the second strain in the step (3) is subjected to the fermentation cultivation in the first fermentation cultivation stage in the second primary fermentation chamber, the third strain is from a third primary fermentation chamber, and the third strain is subjected to the optimal fermentation temperature condition T determined in the step (1)3And optimal Medium Condition S3The duration of the fermentation is less than the optimal fermentation time period condition t3Or at the optimal fermentation temperature condition T3And optimal Medium Condition S3The duration of the new data after the manual intervention adjustment is less than the optimal fermentation duration condition t3The staged fermentation cultivation is carried out; when the second strain in step (3) is subjected to fermentation cultivation in the first secondary fermentation chamber in the first fermentation cultivation stage, the third strain comes from the second primary fermentation chamber, and the third strain is subjected to the optimal fermentation temperature condition T determined in step (1)3And optimal Medium Condition S3The duration of the fermentation is less than the optimal fermentation time period condition t3Or at the optimal fermentation temperature condition T3And optimal Medium Condition S3The duration of the new data after the manual intervention adjustment is less than the optimal fermentation duration condition t3The staged fermentation cultivation of (2).
Preferably, the method for improving the fermentation effect of the feed by controlling the fermentation process of the plurality of strains further comprises a third-stage strain fermentation and cultivation system after the second-stage strain fermentation and cultivation system, wherein the third-stage strain fermentation and cultivation system is connected between the second-stage strain fermentation and cultivation system and the first-stage raw material fermentation system.
Preferably, in the method for improving the fermentation effect of the feed by controlling the fermentation process of the multiple strains, the bin body of each primary fermentation bin and the bin body of each secondary fermentation bin are respectively provided with a heating temperature control device, and the bin body of each raw material fermentation bin is respectively provided with a heating temperature control device.
Preferably, in the method for improving the feed fermentation effect by controlling the fermentation process of the multiple strains, the bin body of each primary fermentation bin comprises a pipeline and a control valve which are communicated with the bin body of the secondary fermentation bin.
Preferably, in the method for improving the feed fermentation effect by controlling the fermentation process of the multiple strains, the cabin body of part or all of the primary fermentation cabins comprises a pipeline and a control valve which are directly communicated with the cabin body of the raw material fermentation cabin.
Preferably, in the method for improving the fermentation effect of the feed by controlling the fermentation process of the multiple strains, the material inlet and the stirring device are arranged on each of the primary fermentation bin, the secondary fermentation bin and the raw material fermentation bin.
The invention has the beneficial effects that:
the invention provides a method for improving the fermentation effect of feed by controlling the fermentation process of multiple strains, provides a production-oriented industrialized scheme aiming at staged composite strain fermentation culture, and before the whole set of production system and the scheme thereof are provided, the production-oriented multi-strain mixed fermentation feed preparation process route mainly adopts two routes of directly mixing a material to be fermented after single-strain fermentation culture or carrying out mixed fermentation culture on a plurality of strains obtained by expanded culture and then mixing the material to be fermented, the process route provided by the invention solves the production problem in staged composite strain fermentation culture, determines complete set of operation data and parameters in the experiment and data acquisition stages, and programs the operation data and parameters for the automatic multistage fermentation system to call and organize production, the method can realize the conversion from research to industry of staged composite strain fermentation cultivation, can adjust experimental data and parameters on line, and can select a staged mixed fermentation on-line mode according to the occupation and idle conditions of required strains and various chambers of equipment, and one set of production system can almost meet all common composite fermentation requirements in production.
Drawings
The aspects and advantages of the present application will become apparent to those of ordinary skill in the art upon reading the following detailed description of the preferred embodiments. The drawings are only for purposes of illustrating the preferred embodiments and are not to be construed as limiting the invention. In the drawings:
FIG. 1 is a flowchart illustrating the operation of a method for improving the fermentation effect of feed by controlling the fermentation process of multiple strains according to various embodiments of the present invention, wherein S1-S4 correspond to steps (1) - (4) of the present invention.
FIG. 2 is a schematic diagram showing the configuration of a multi-stage fermentation system used in the method for improving the fermentation effect of a feed by controlling the fermentation process of a plurality of bacterial strains according to example 1 of the present invention.
FIG. 3 is a schematic diagram showing the configuration of a multi-stage fermentation system used in the method for improving the fermentation effect of a feed by controlling the fermentation process of a plurality of bacterial strains according to example 2 of the present invention.
In the figure:
the system comprises a first secondary fermentation bin 10, a first secondary fermentation bin material inlet 11, a first secondary fermentation bin heating temperature control device 12, a first secondary fermentation bin outlet control valve 13 and a first secondary fermentation bin inlet control valve 14;
the system comprises a first primary fermentation bin 20, a first primary fermentation bin material inlet 21, a first primary fermentation bin heating temperature control device 22 and a first primary fermentation bin outlet control valve 23;
a second primary fermentation bin 30, a second primary fermentation bin material inlet 31, a second primary fermentation bin heating temperature control device 32 and a second primary fermentation bin outlet control valve 33;
a third primary fermentation chamber 40, a third primary fermentation chamber material inlet 41, a third primary fermentation chamber heating temperature control device 42 and a third primary fermentation chamber outlet control valve 43;
the device comprises a first primary raw material fermentation bin 50, a first primary raw material fermentation bin material inlet 51, a first primary raw material fermentation bin heating temperature control device 52, a discharge control valve 53 and a first primary raw material fermentation bin inlet control valve 54.
Detailed Description
Exemplary embodiments of the present disclosure will be described in more detail below with reference to the accompanying drawings.
Example 1
A method for improving the fermentation effect of feed by controlling the fermentation process of multiple strains is shown in figure 1, and comprises the following process routes:
(1) experiment and data acquisition
The strains commonly used in production are selected, and the strains are marked as a first strain, a second strain and a third strain respectively by taking the minimum three strains as examples.
The experiment confirms the respective optimal fermentation of each strainAnd (3) culturing conditions, wherein the fermentation culturing conditions at least comprise a fermentation temperature, a fermentation duration condition and a culture medium condition, and respectively record: i the optimum fermentation temperature condition of the first strain is T1The optimal fermentation time period condition is t1The optimal culture medium condition is S1(ii) a II the optimum fermentation temperature condition of the second strain is T2The optimal fermentation time period condition is t2The optimal culture medium condition is S2(ii) a III the optimal fermentation temperature condition of the third strain is T3The optimal fermentation time period condition is t3The optimal culture medium condition is S3。
The method comprises the following steps of determining optimal conditions for staged composite strain fermentation cultivation by experiments, wherein the staged composite strain fermentation cultivation at least comprises a first fermentation cultivation stage and a second fermentation cultivation stage, the first fermentation cultivation stage is a single strain fermentation cultivation stage and at least comprises a first fermentation cultivation stage of a first strain and a first fermentation cultivation stage of a second strain, the second fermentation cultivation stage is a mixed fermentation cultivation stage of more than two strains and at least comprises a mixed fermentation cultivation stage of the first strain and the second strain, and the optimal conditions determined by the experiments at least comprise: i optimal fermentation time length t of first fermentation cultivation stage of first strain1' and the optimal fermentation time t of the first fermentation cultivation stage of the second species2', wherein, t1′＜t1，t2′＜t2(ii) a II optimum fermentation temperature condition T of the second fermentation cultivation stage of the mixed strain of the first strain and the second straineAnd an optimum fermentation time te。
Since the main objective of the present invention is to provide a production-oriented industrial solution, it is an important starting point to use a set of production system to satisfy all the common complex fermentation requirements in production, in actual production, the data collected in step (1) is not only the optimal fermentation cultivation data for one product, but also the data that can be called at any time for multiple products, and the types and the quantities of the strains used for complex fermentation are different in different products, therefore, the above-collected optimal condition data for the staged complex strain fermentation cultivation of the first and second strains are only the example data, and the strains used for complex fermentation may have other combination modes, such as the combination of the first and third strains, the combination of the second and third strains, according to the product, therefore, in step (1), the optimal conditions of the staged composite strain fermentation cultivation are also determined by experiments aiming at the composite fermentation cultivation of the first strain and the third strain and the composite fermentation cultivation of the second strain and the third strain, and the optimal conditions at least comprise the optimal fermentation time length of the first fermentation cultivation stage at the first point, and the optimal fermentation temperature condition and the optimal fermentation time length of the second fermentation cultivation stage at the second point, so that the production system can call the data at any time when the product is replaced.
(2) Production data entry
Recording all the optimal conditions determined in the experiment of the step (1) into a production management system, providing online raw data for the production of the fermented feed, and writing data used in the next operation into a control panel of the production control system for providing an executable control program for the production of the fermented feed.
(3) Organizing production using a multi-stage fermentation system
Producing fermented feed by using an automatic fermented feed production system according to the production data recorded in the step (2), wherein as shown in fig. 2: the automatic fermented feed production system comprises a two-stage strain fermentation and cultivation system and a one-stage raw material fermentation system, wherein the first-stage strain fermentation and cultivation system comprises three one-stage fermentation bins, namely a first primary fermentation bin 20, a second primary fermentation bin 30 and a third primary fermentation bin 40, the second-stage strain fermentation and cultivation system comprises a first secondary fermentation bin 10, the one-stage raw material fermentation system comprises a first primary raw material fermentation bin 50, and the three one-stage fermentation bins, the one-stage fermentation bin and the one-stage raw material fermentation bin can provide sufficient composite fermentation and segmented implementation conditions for strains commonly used in most probiotic feed products of enterprises.
During production, in the first primary fermentation chamber 20Performing fermentation cultivation on the first strain according to the determined optimal fermentation temperature condition and optimal culture medium condition of the first strain, wherein the cultivation time is the optimal fermentation time t of the first fermentation cultivation stage of the first strain determined in the step (1)1', the optimal fermentation temperature condition and the optimal culture medium condition are respectively the optimal fermentation temperature condition T of the first strain determined in the step (1)1And optimal Medium Condition S1(since the stepwise fermentation is carried out here, the optimum fermentation temperature condition T of the first bacterial species determined in step (1) may be used1And optimal Medium Condition S1Using the fine-tuned new data after manual intervention adjustment); fermenting and culturing the second strain in the second primary fermentation bin 30 and/or the first secondary fermentation bin 10 according to the determined optimal fermentation temperature condition and optimal culture medium condition of the second strain, wherein the culture time is the optimal fermentation time t of the first fermentation culture stage of the second strain determined in the step (1)2', the optimal fermentation temperature condition and the optimal culture medium condition are respectively the optimal fermentation temperature condition T of the second strain determined in the step (1)2And optimal Medium Condition S2(fine tuning is also possible).
Setting the initial fermentation and cultivation time of the first strain and the second strain to ensure that the time length t of the first fermentation and cultivation stage of the first strain1Period of and length of first fermentation incubation period t of the second species2' then, the first and second strains completing the first fermentation incubation stage are mixed by operating the control valve for a time period teThe second fermentation cultivation stage (2).
With the system shown in fig. 2, the second strain can be fermented and cultivated in the first stage in the second first-stage fermentation chamber 30, or directly fermented and cultivated in the first stage in the first second-stage fermentation chamber 10, the latter scheme occupies less equipment, but the former scheme can make full use of the space of the chamber body for capacity design, and the mixed fermentation condition of the latter stage can be arranged in advance before entering the mixed fermentation of the latter stage. When the second strain is in the second primary fermentation chamber 30During the fermentation cultivation in the first fermentation cultivation stage, the first strains in the first primary fermentation bin 20 and the second strains in the second primary fermentation bin 30 flow into the first secondary fermentation bin 10 by opening the control valve on the path leading from the first primary fermentation bin 20 and the second primary fermentation bin 30 to the first secondary fermentation bin 10 and are mixed in the first secondary fermentation bin 10 for the duration teThe second fermentation cultivation stage (2). As can be seen from fig. 2, the control valve on the path from the first primary fermentation chamber 20 and the second primary fermentation chamber 30 to the first secondary fermentation chamber 10 includes two pipeline stop valves disposed on the common pipeline of the three primary fermentation chambers, in addition to the first primary fermentation chamber outlet control valve 23, the second primary fermentation chamber outlet control valve 33, and the first secondary fermentation chamber inlet control valve 14, and disposed between the first primary fermentation chamber 20 and the second primary fermentation chamber 30, and between the second primary fermentation chamber 30 and the third primary fermentation chamber 40, respectively, and the pipeline stop valves are used to prevent the excessive bacteria liquid remaining in the pipeline when different primary fermentation chambers are used for tissue production.
When the second strain is fermented and cultivated in the first secondary fermentation chamber 10 in the first fermentation cultivation stage, the first strain in the first primary fermentation chamber 20 and the second strain originally existing in the first secondary fermentation chamber 10 are mixed by opening a control valve on a path leading from the first primary fermentation chamber 20 to the first secondary fermentation chamber 10 and are fermented and cultivated in the first secondary fermentation chamber 10 for the time period teThe second fermentation cultivation stage, the control valves to be opened at this time are the first primary fermentation bin outlet control valve 23, the first secondary fermentation bin inlet control valve 14 and the two pipeline stop valves. In the second fermentation cultivation stage, the optimum fermentation temperature condition T determined in the step (1) is selected as the fermentation temperature conditione。
The above time period is teAfter the second fermentation cultivation stage, according to a predetermined strain fermentation system, the mixed strain obtained in the first secondary fermentation chamber 10 can be directly introduced into the first primary raw material fermentation chamber 50 connected to the first secondary fermentation chamber 10, and mixed with the fermentation raw material to prepare the fermented feed. In this example, three strains are mixed and fermented in stagesFor example, the third strain may be fermented and cultured in advance in the idle fermentation chamber shown in fig. 2, so that the mixed strain obtained in the first secondary fermentation chamber 10 may be further mixed with the fermented and cultured third strain and then introduced into the first primary raw material fermentation chamber 50, or the mixed strain obtained in the first secondary fermentation chamber 10 may be mixed with the third strain that has only been subjected to the stage fermentation and culture, and introduced into the first primary raw material fermentation chamber 50 after a new mixed fermentation and culture stage, which may be determined according to the strain mixed fermentation scheme required by the current fermented feed determined by the production experiment.
The use and arrangement of the empty fermentation tanks is illustrated here by way of a preferred example: when the second seed in step (3) is subjected to said first fermentation incubation stage in the second primary fermentation tank 30, said third seed is from the empty third primary fermentation tank 40, said third seed having the optimum fermentation temperature condition T determined in said step (1)3And optimal Medium Condition S3(fine tuning possible) is carried out for a duration less than the optimal fermentation time period condition t3The staged fermentation cultivation is carried out; when the second strain of step (3) is subjected to the fermentative cultivation of the first fermentative cultivation stage in the first secondary fermentation tank 10, the third strain is from the second primary fermentation tank 30, and the third strain is subjected to the optimal fermentative temperature condition T determined in step (1)3And optimal Medium Condition S3(fine tuning possible) is carried out for a duration less than the optimal fermentation time period condition t3The staged fermentation cultivation of (2).
It should be noted that, according to the laboratory research data, the optimal conditions of the multi-strain mixed fermentation stage are directly related to the number of strains, so that it can be verified that the optimal conditions are different from those of the mixed fermentation system consisting of only the first and second strains to some extent when the third strain is added into the mixed fermentation system of the first and second strains. Therefore, it is preferable that the mixed strain obtained in the first secondary fermentation chamber 10 is only staged when the three-strain mixed system is selected for productionWhen the third strains are mixed and a new mixed fermentation cultivation stage is carried out, the optimal fermentation time t of the first fermentation cultivation stage for collecting the third strains is increased in the experiment and data collection stage of the step (1)3′(t3′＜t3) And the optimum fermentation temperature condition T of the second fermentation cultivation stage of the originally collected mixed strain of the first strain and the second straineAnd an optimum fermentation time teThe method does not need to calculate the final data of the fermentation and cultivation stage, but process data, wherein the parameter symbol is unchanged, but the parameter value is changed with high probability, and the possibility of linkage of the parameter values of the first strain and the second strain in the first fermentation and cultivation stage is not eliminated. Finally, it is necessary to collect the most important data (which may be labeled as T respectively) of the optimal fermentation temperature and the optimal fermentation time in the third fermentation cultivation stage of the first, second and third mixed strainse2And te2)。
According to the above description, it is an important starting point of the present invention to use a set of production system to satisfy all the commonly used complex fermentation requirements in production, and the strains to be used in complex first may be other combinations according to different products, therefore, in the above step (1), the optimal conditions for staged complex strain fermentation cultivation are determined by the complex fermentation cultivation for the first strain and the third strain, and the complex fermentation cultivation for the second strain and the third strain, correspondingly, when the subsequent mixed cultivation with the rest one strain (the second strain, the first strain) is performed similar to the above mentioned three strains, the optimal fermentation duration data of the first fermentation cultivation stage for collecting the rest one strain (the second strain, the first strain) under the corresponding scheme is also increased in the experiment and data collection stage of step (1), supplementing the optimal fermentation temperature condition and the optimal fermentation duration data of the second fermentation cultivation stage of the first two strains which may be changed, even the optimal fermentation duration data of the first two strains in the first fermentation cultivation stage which are changed, and supplementing the data in a way of adding a new record in a database, wherein the optimal fermentation temperature condition and the optimal fermentation duration data of the third fermentation cultivation stage of the last mixed strain of the first strain, the second strain and the third strain also need to be added with an updated record, because the fermentation cultivation sequences of the strains in the three schemes are actually different, the conditions of the fermentation cultivation can also be different to a certain extent.
(4) Discharge and after-treatment
After the fermentation of the raw material in the first primary raw material fermentation bin 50 is completed, the raw material is released and the subsequent processing is performed by operating the discharge control valve 53 of the first primary raw material fermentation bin 50. The release of the raw material and the subsequent treatment are not limited to the collection and treatment of the fermented feed product, and new process treatments (mixing with other materials and fermentation processes are not excluded) may be introduced subsequently as required.
In the multi-stage fermentation system described above, the hardware configuration of the strain fermentation and cultivation system is not limited to the two stages shown in the figure, and a third stage strain fermentation and cultivation system may be further provided after the second stage strain fermentation and cultivation system, and the third stage strain fermentation and cultivation system is connected between the second stage strain fermentation and cultivation system and the first stage raw material fermentation system, or may be arranged in a three-dimensional space in a layered manner as shown in fig. 2, so as to realize or promote the circulation of the material by means of gravity conditions.
Because the bin body of each primary fermentation bin and the bin body of the secondary fermentation bin in the multistage fermentation system are respectively provided with a heating temperature control device, and the bin body of each raw material fermentation bin is also provided with a heating temperature control device, the optimal cultivation temperature is provided for the fermentation cultivation of different strains in each stage and the important effect is played on the cultivation temperature stabilization, because the bin body of each primary fermentation bin comprises a pipeline and a control valve which are communicated with the bin body of the secondary fermentation bin and can share the pipeline, the construction of various passages is realized, the equipment is saved, the waste of bacteria liquid is prevented, in addition, each primary fermentation bin, the secondary fermentation bin and the bin body of the raw material fermentation bin of the multistage fermentation system are also respectively provided with a material inlet and a stirring device, although only a single material inlet is schematically shown on each bin body, according to the difference of the added materials, such as bacteria liquid, culture medium materials, auxiliary materials, raw materials to be fermented in different states and the like, and material inlets with different sizes and structures can be arranged on each bin body.
Example 2
The embodiment is further perfected on the basis of the embodiment 1, in the production, the fermentation cultivation of seed liquid, the fermentation cultivation of mixed strains and the fermentation process of feed occupy relatively long time, the production plan is also variable, and according to the different types and the different production discharge modes of the produced products, a single strain or a mixed strain in a certain cabin space in the fermentation process can break through the originally set staged mixed fermentation cultivation mode for new use, therefore, the embodiment further builds up a pipeline and a control structure, the cabin bodies of all the primary fermentation cabins are provided with pipelines and control valves which directly lead to the cabin bodies of the raw material fermentation cabins, and the pipelines and the control valves comprise pipeline stop valves on a common pipeline, so that when needed, any one or more of the first primary fermentation cabin 20, the second primary fermentation cabin 30 and the third primary fermentation cabin 40 can directly supply the fermented strains to the first primary raw material fermentation cabin 50, any one or more of the first primary fermentation chamber 20, the second primary fermentation chamber 30 and the third primary fermentation chamber 40 can also supply fermentation strains to the first primary raw material fermentation chamber 50 together with the first secondary fermentation chamber 10.
The above description is only a preferred embodiment of the present invention, and the protection scope of the present invention shall be subject to the protection scope of the appended claims.
Claims (9)
1. A method for improving the fermentation effect of feed by controlling the fermentation process of multiple strains is characterized by comprising the following process routes:
(1) experiment and data acquisition
Selecting strains commonly used in production, wherein at least three strains are marked as a first strain, a second strain and a third strain respectively;
the respective optimal fermentation and cultivation conditions of each strain are determined through experiments, the fermentation and cultivation conditions at least comprise fermentation temperature, fermentation duration conditions and culture medium conditions, and the following are recorded respectively: i the optimum fermentation temperature condition of the first strain is T1The optimal fermentation time period condition is t1The optimal culture medium condition is S1(ii) a II the optimum fermentation temperature condition of the second strain is T2The optimal fermentation time period condition is t2The optimal culture medium condition is S2(ii) a III the optimal fermentation temperature condition of the third strain is T3The optimal fermentation time period condition is t3The optimal culture medium condition is S3；
The method comprises the following steps of determining optimal conditions for staged composite strain fermentation cultivation by experiments, wherein the staged composite strain fermentation cultivation at least comprises a first fermentation cultivation stage and a second fermentation cultivation stage, the first fermentation cultivation stage is a single strain fermentation cultivation stage and at least comprises a first fermentation cultivation stage of a first strain and a first fermentation cultivation stage of a second strain, the second fermentation cultivation stage is a mixed fermentation cultivation stage of more than two strains and at least comprises a mixed fermentation cultivation stage of the first strain and the second strain, and the optimal conditions determined by the experiments at least comprise: i optimal fermentation time length t of first fermentation cultivation stage of first strain1' and the optimal fermentation time t of the first fermentation cultivation stage of the second species2', wherein, t1′＜t1，t2′＜t2(ii) a II optimum fermentation temperature condition T of the second fermentation cultivation stage of the mixed strain of the first strain and the second straineAnd an optimum fermentation time te；
(2) Production data entry
Recording all or part of the optimal conditions determined in the experiment of the step (1) into a production management system and/or a production control system, wherein the production management system can provide online original data for the production of the fermented feed, and the production control system can directly provide an executable control program for the production of the fermented feed;
(3) organizing production using a multi-stage fermentation system
Producing fermented feed by using an automatic fermented feed production system according to the production data entered in the step (2), wherein:
the automatic fermented feed production system comprises at least two stages of strain fermentation and cultivation systems and at least one stage of raw material fermentation system, wherein the first stage of strain fermentation and cultivation system comprises at least two first-stage fermentation bins (20) and a second-stage fermentation bin (30), the second-stage strain fermentation and cultivation system comprises at least one first second-stage fermentation bin (10), the first-stage raw material fermentation system comprises at least one first-stage raw material fermentation bin (50), during production, the first strain is subjected to fermentation cultivation in the first-stage fermentation bin (20) according to the determined optimal fermentation temperature condition and optimal culture medium condition of the first strain, and the cultivation duration is the optimal fermentation duration t of the first fermentation and cultivation stage of the first strain determined in the step (1)1', the optimal fermentation temperature condition and the optimal culture medium condition are respectively the optimal fermentation temperature condition T of the first strain determined in the step (1)1And optimal Medium Condition S1Or according to the optimal fermentation temperature condition T of the first strain determined in the step (1)1And optimal Medium Condition S1Performing manual intervention on the adjusted new data; fermenting and culturing the second strain in the second primary fermentation bin (30) and/or the first secondary fermentation bin (10) according to the determined optimal fermentation temperature condition and optimal culture medium condition of the second strain, wherein the culture time is the optimal fermentation time t of the first fermentation culture stage of the second strain determined in the step (1)2', the optimal fermentation temperature condition and the optimal culture medium condition are respectively the optimal fermentation temperature condition T of the second strain determined in the step (1)2And optimal Medium Condition S2Or according to the optimal fermentation temperature condition T of the first strain determined in the step (1)2And optimal Medium Condition S2Performing manual intervention on the adjusted new data;
setting the initial fermentation and cultivation time of the first strain and the second strain to ensure that the time length t of the first fermentation and cultivation stage of the first strain1Period of and length of first fermentation incubation period t of the second species2' then, the first and second strains completing the first fermentation incubation stage are mixed by operating the control valve for a time period teWhen the second strain is fermented and cultivated in the first fermentation and cultivation stage in the second primary fermentation bin (30), the first strain in the first primary fermentation bin (20) and the second strain in the second primary fermentation bin (30) flow into the first secondary fermentation bin (10) by opening a control valve on a path leading from the first primary fermentation bin (20) and the second primary fermentation bin (30) to the first secondary fermentation bin (10) and are mixed in the first secondary fermentation bin (10) for the duration teThe second fermentation cultivation stage of (1); when the second strain is fermented and cultivated in the first secondary fermentation chamber (10) in the first fermentation cultivation stage, the first strain in the first primary fermentation chamber (20) and the second strain originally existing in the first secondary fermentation chamber (10) are mixed by opening a control valve on a path leading from the first primary fermentation chamber (20) to the first secondary fermentation chamber (10) and are fermented and cultivated in the first secondary fermentation chamber (10) for the time period teThe second fermentation cultivation stage of (1); in the second fermentation cultivation stage, the optimum fermentation temperature condition T determined in the step (1) is selected as the fermentation temperature conditione；
The above time period is teAfter the second fermentation cultivation stage, directly introducing the mixed strain obtained in the first secondary fermentation bin (10) into a first primary raw material fermentation bin (50) connected with the first secondary fermentation bin (10) to perform mixed fermentation with fermentation raw materials to prepare fermented feed, or mixing the mixed strain obtained in the first secondary fermentation bin (10) with one or more other strains which are already fermented and cultivated and then introducing the mixed strain into the first primary raw material fermentation bin (50), or mixing the mixed strain obtained in the first secondary fermentation bin (10) with one or more other strains which are already subjected to stage fermentation cultivation and introducing the mixed strain into the first primary raw material fermentation bin (50) after a new mixed fermentation cultivation stage;
(4) discharge and after-treatment
After the fermentation of the raw materials in the first primary raw material fermentation bin (50) is finished, the raw materials are released by operating a discharge control valve (53) of the first primary raw material fermentation bin (50) and are subjected to subsequent treatment.
2. The method for improving fermentation efficiency of feedstuff by controlling fermentation process of multiple strains as claimed in claim 1, wherein the staged fermentation culture of the multiple strains in step (1) further comprises at least a third fermentation culture stage, in which the mixed strain obtained from the second fermentation culture stage is mixed with one or more other strains which have not been fermented or have been fermented only in the stage.
3. The method for improving fermentation efficiency of feedstuff by controlling fermentation process of multiple bacterial strains according to claim 2, wherein the said another one or more bacterial strains which are not cultured by fermentation or are cultured by fermentation only in stage (1) comprise the third bacterial strain.
4. A method for improving fermentation efficiency of feed stuff by controlling fermentation process of multiple strains according to claim 3, wherein when the second strain in step (3) is subjected to fermentation cultivation in the first fermentation cultivation stage in the second primary fermentation chamber (30), the third strain is from a third primary fermentation chamber (40) under the optimum fermentation temperature condition T determined in step (1)3And optimal Medium Condition S3The duration of the fermentation is less than the optimal fermentation time period condition t3Or at the optimal fermentation temperature condition T3And optimal Medium Condition S3The duration of the new data after the manual intervention adjustment is less than the optimal fermentation duration condition t3The staged fermentation cultivation is carried out; when the second strain in the step (3) is subjected to fermentation cultivation in the first secondary fermentation chamber (10), the third strain comes from the second primary fermentation chamber (30), and the third strain comes from the second primary fermentation chamber (30)The strain is cultured under the optimal fermentation temperature condition T determined in the step (1)3And optimal Medium Condition S3The duration of the fermentation is less than the optimal fermentation time period condition t3Or at the optimal fermentation temperature condition T3And optimal Medium Condition S3The duration of the new data after the manual intervention adjustment is less than the optimal fermentation duration condition t3The staged fermentation cultivation of (2).
5. The method for improving fermentation efficiency of feedstuff by controlling fermentation process of multiple strains as claimed in claim 1, further comprising a third-stage strain fermentation cultivation system after the second-stage strain fermentation cultivation system, wherein the third-stage strain fermentation cultivation system is connected between the second-stage strain fermentation cultivation system and the primary raw material fermentation system.
6. The method for improving the fermentation effect of the feed by controlling the fermentation process of the multiple strains according to any one of claims 1 to 5, wherein the chamber body of each primary fermentation chamber and the chamber body of each secondary fermentation chamber are provided with a heating temperature control device, and the chamber body of each raw material fermentation chamber is provided with a heating temperature control device.
7. The method for improving the fermentation effect of the feed by controlling the fermentation process of the multiple strains according to any one of claims 1 to 6, wherein the chamber body of each primary fermentation chamber comprises a pipeline and a control valve which are communicated with the chamber body of the secondary fermentation chamber.
8. The method for improving the fermentation effect of the feed by controlling the fermentation process of the multiple strains according to any one of claims 1 to 7, wherein the chamber body of part or all of the primary fermentation chambers comprises a pipeline and a control valve which are directly communicated with the chamber body of the raw material fermentation chamber.
9. The method for improving the fermentation effect of the feed by controlling the fermentation process of the multiple strains according to any one of claims 1 to 8, wherein a material inlet and a stirring device are arranged on each of the primary fermentation bin, the secondary fermentation bin and the raw material fermentation bin.
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| KR20110095487A (en) * | 2010-02-19 | 2011-08-25 | 노정만 | How to prepare pig feed using waste mushroom medium as main ingredient |
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| CN105265769A (en) * | 2015-10-22 | 2016-01-27 | 李德舜 | Multifunctional bacterium fermented compound enzyme feed and preparation method thereof |
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| CN112425684A (en) * | 2020-11-10 | 2021-03-02 | 山东泰山生力源集团股份有限公司 | Fermented feed additive with immunity enhancing effect and preparation method thereof |
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| KR20110095487A (en) * | 2010-02-19 | 2011-08-25 | 노정만 | How to prepare pig feed using waste mushroom medium as main ingredient |
| CN102178036A (en) * | 2011-04-22 | 2011-09-14 | 河南宏翔生物科技有限公司 | Method for producing high-methionine and high-lysine feed by using composite bacteria fermented corn protein powder |
| CN105265769A (en) * | 2015-10-22 | 2016-01-27 | 李德舜 | Multifunctional bacterium fermented compound enzyme feed and preparation method thereof |
| CN105941828A (en) * | 2016-05-16 | 2016-09-21 | 内蒙古工业大学 | Method for producing compound feed from mixed strains through united fermentation |
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