CN1036146C - Multi-layer separated biological reactor and method of production of alcohol with fermented mellow corn using same - Google Patents
Multi-layer separated biological reactor and method of production of alcohol with fermented mellow corn using same Download PDFInfo
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- CN1036146C CN1036146C CN92109880A CN92109880A CN1036146C CN 1036146 C CN1036146 C CN 1036146C CN 92109880 A CN92109880 A CN 92109880A CN 92109880 A CN92109880 A CN 92109880A CN 1036146 C CN1036146 C CN 1036146C
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Abstract
The present invention relates to a multi-layer separated biological reactor. Screen mesh particle baskets made of metal or rigid plastics, and resin, etc. are placed in a fermentation tank; immobilized yeast gel is put in the particle baskets. When the multi-layer particle baskets are adopted, a certain gap is arranged among the multi-layer particle baskets, and an immobilized cell technology is adopted by making use of the reactor. The technology for preparing alcohol by corn mash is immobilized proliferation yeast gel which is prepared by selecting polyvinyl alcohol as a carrier, and fermentation is carried out at the fermentation temperature of 35 to 40 DEG C and under the condition of the PH value of 3.5 to 6.0. The present invention has the advantages of simple operation, high reaction speed, short fermentation period, easy control, little pollution and long service life of yeast activity. Compared with that of the traditional technology, the production capacity can be increased by more than 3 times.
Description
The present invention is for biological fermentation engineering provides a kind of fixed bed multi-layer separated biological device that is suitable for phase reaction (solid, liquid, gas), provides simultaneously that a kind of to utilize this reactor be the novel process of raw material production alcohol with corn, cereal or potato class converted mash.
After sixties enzyme immobilization technology and the immobilized cell technology of the multienzymatic reaction that rises has been widely used in industrial fermentation, medicine, fields such as environment protection, analytical chemistry and vegeto-animal tissue culture.The application of immobilized cell technology aspect fermentation industry further promoted development of biology.Fixation of microbial cell since its preparation fairly simple, with low cost, can activate repeatedly, life-time service has been opened the gate of biofermentation technique serialization automatic production.The key issue that realizes above-mentioned immobilized cell technique production is to select suitable fixation support and design bio-reactor, and wherein the selection of fixation support is mainly selected by producing used raw material, makes it adapt to fermentation technology process.And bio-reactor mainly is to depend on the actual production effect, and can its decision immobilized cell technology be applied in traditional production.In recent years, more and more many to the research of bio-reactor both at home and abroad.Be used for that the reactor of fermentation industry is blanket can be divided into two big classes: i.e. fluidized-bed reactor and fixed-bed reactor, but this class capital cost of reactor many, be difficult to process, technology complexity.In addition, the present fermentation industry of China all is traditional general form fermentor tank basically.Though this explained hereafter ability is low, fermentation period is long, abandon existing traditional zymotic equipment, and dropping into a large amount of funds also is unpractical in order to set up new-type bio-reactor.Therefore, designing a kind of easy bio-reactor, transform the existing fermentation industry equipment of China, realize immobilized cell technology, make fermentative production break through traditional pattern, is a problem demanding prompt solution in the biotechnology.
The objective of the invention is to design a kind of new-type bioreactor, traditional universal old-fashioned fermentation equipment is transformed.In addition, another task of the present invention is to utilize above-mentioned reactor, for the converted mash with corn cereal and potato class is a raw material, select suitable fixation support, realize immobilized cell technology production alcohol, a kind of novel process that adopts the fermented maize saccharification producing spirit with undecanted wine of immobilized cell technology promptly is provided, and in fermentation industry, is used widely.
For achieving the above object, the present invention has designed a kind of multi-layer separated biological device, specifically, multi-layer separated biological device of the present invention, it is characterized in that being equipped with one or more layers particle basket in fermentor tank (general form fermentor tank), the particle basket is the screen basket made from materials such as metals (or hard polymer resin, plastics), puts into the fixed yeast gelinite in the screen basket, make gelinite can not flow out the particle basket, but fermented liquid can be by the free fluidisation of screen cloth of particle basket.The mesh size of particle basket screen cloth can be decided by the volume of cell immobilization carrier performance of choosing and the gel grain of making, and generally can adopt 1～100 millimeter
2Mesh.When adopting the multilayered particles basket, should leave certain intervals between each layer particle basket, be beneficial to the gas purging that fermented liquid produces in fluidisation and the fermenting process in jar.In addition, also can in the particle basket, (center or even) one or several vapor pipes be set.Accompanying drawing 1 is the structural representation of multi-layer separated biological device of the present invention; Accompanying drawing 2 is the structural representation of particle basket; Give to illustrate in further detail below in conjunction with 1 pair of structure of reactor of the present invention of accompanying drawing.
Among the figure: 1, fermentor tank; 2, particle basket; 3, vapor pipe; 4, support; 5, interlayer; 6, opening for feed; 7, discharge port; 8, metallic sieve basket; 9, screen basket lid.By the multi-layer separated biological device of the present invention shown in Fig. 1,2 is to place in fermentor tank 1, by metallic sieve basket 8 and the screen basket lid 9 particle baskets of making 2, be placed with vapor pipe 3 with holes in the particle basket 2, Figure 1 shows that four layers of particle basket, leave a determining deviation between each particle basket, vapor pipe 3 had both played the effect of emission gases, and also as the support column of particle basket 2, particle basket 2 feeds the support placement and is fixed in the fermentor tank 1.The outer interlayer 5 of fermentor tank is its heat exchange layers, feeds water 9 or gas) make fermentor tank remain on certain leavening temperature.Raw material is sent into by the opening for feed 6 of fermentor tank 1, and the fermentation after product is discharged by discharge port 7.Above-mentioned is fixed multi-layer separated biological device of the present invention.Can be used for various fermenting processs.Simultaneously, also can transform, in former fermentor tank, put into the particle basket for preparing, promptly carry out fermentative production alcohol applicable to immobilized cell technology to the traditional zymotic equipment of present widespread usage.
The present invention adopts above-mentioned multi-layer separated biological device, for being that the production process of fermenting raw materials alcohol provides a kind of new technology with corn, cereal and potato class etc.Its technical process is described in detail its processing method below in conjunction with accompanying drawing 3 as shown in Figure 3.
Among Fig. 3: 11, seed culture: 12, carrier; 13, fixed yeast gelinite preparation; 14, Yeast proliferation is cultivated; 15, raw material is handled; 16, saccharification; 17, fermentation reaction; 18, distillation; 19, finished product.
By biomass to alcohol conversion process flow process shown in Figure 3, the preparation process of its fixed yeast gelinite adopts glucose, peptone, yeast extract paste, MgSO at first getting the yeast saccharomyces cerevisiae bacterial classification
47H
2O, KH
2PO
412H
2The substratum of compositions such as O carries out seed culture, i.e. operation 11 seed culture; 14 operations of cultivating preparation 13 of fixed yeast gelinite and Yeast proliferation adopt corn mash liquid to make substratum, its fixed yeast gelinite preparation section is characterised in that selects for use polyvinyl alcohol (PVA) as carrier 12, and the bacterium number in the fixed yeast gelinite of S. cervisiae after above-mentioned 11～14 operations are handled is no less than 10
6Individual/ml, and its gelinite is put into the particle basket of reactor.Because in immobilized cell fermentation technology, what of fixed yeast gelinite (solid holdup) have tangible influence to the productivity of alcohol in the reactor, suitably strengthen solid holdup, though alcohol speed is fast, but productivity is lower, and the reactor inner fluid mass transfer effect that solid holdup is low is good, but production alcohol speed is slow, solid holdup is 7～20% in the general fermenting process, and preferable solid holdup is 7～15%.The treatment process 15 and the saccharification operation 16 of preparation alcohol raw material corn can be carried out with reference to the routine operation method.But because fixed hyperplasia cell fermentation technology, increased cell density and quickened fermentation reaction, thereby shortened fermentation period greatly.Therefore require the conversion coefficient of saccharification operation to want high (initial fermentability pol height), can finish fermentation in the short period of time fast, the conversion coefficient of its corn mash should be more than 80%.Saccharification 16 is reflected under 58～60 ℃ to be carried out, and mash carries out fermentation reaction after the saccharification in bio-reactor provided by the invention is sent in cooling, and its fermentation period is short, improves Alcohol Production power, and can realize semicontinuous fermentation.Its fermentation reaction temperature is controlled at 25～40 ℃, and the pH value is controlled at 3.5～6.0.Behind above-mentioned fermentation reaction 17, karusen distills 18 processing can obtain finished product alcohol 19.Its treatment process is identical with the traditional technology method.Give further instruction below by embodiment to technology of the present invention.
Embodiment 1, the multi-layer separated biological device
Structure fabrication volume of the present invention by reactor shown in the accompanying drawing 1,2 is the reactor of 1000 liters, it adopts 4 layers of particle basket, long-pending 30%～40% of the fermentor tank volume that accounts for of particle basket, the particle basket is made with Stainless Steel Wire Sieve, mesh is 5 * 5 millimeter 2, it is 100 millimeters porous steel pipe that there is an internal diameter at particle basket center, and as vapor pipe, four layers of particle basket are separately fixed on the porous steel pipe.The particle basket is placed on to be fixed on the support makes the multi-layer separated biological device in the fermentor tank.
Embodiment 2, fixed hyperplasia yeast fermentation corn mash preparing alcohol
Utilizing embodiment 1 described volume is the reactor of 1000 liters, is raw material with the corn, and prepares alcohol by technical process shown in Figure 3.The preparation of its fixed yeast gelinite, selecting polyvinyl alcohol (PVA) is carrier, yeast saccharomyces cerevisiae is handled through 11～14 operations and is made fixed hyperplasia yeast gelinite, and by 7% solid holdup the gelinite five equilibrium is packed in four particle baskets of reactor.Handle maize raw material by the traditional technology method, its saccharifying carries out under 58～60 ℃, and the conversion coefficient of corn mash is 80%.Mash after the saccharification is cooled to 30 ℃ and sends in the reactor, and leavening temperature is 33～35 ℃, and the free cell number in the mash is 10
6～10
7Individual/ml, the cell bud ratio is about 13%.The initial pol of mash is 17～20%, and fermentation reaction carried out 20 hours, and fermentation back mash residual sugar is 0.2～0.7%, ethanol content 9.0～11.8%V/V, and its theoretical yield is greater than 90%.2 months intravital yeast fermentation activity of its immobilized gel are carried out in the serialization of above-mentioned alcohol preparation process does not still have considerable change.
Multi-layer separated biological device by the invention described above can satisfy the requirement of immobilized cell technology to reactor, and its device fabrication is simple.Utilize this reactor, and to select polyvinyl alcohol for use be the fixed hyperplasia yeast gelinite that carrier is made, be used for the corn producing spirit with undecanted wine, its technology operation is simple, and is reliable and stable, is easy to amplify, compare with the traditional zymotic process, when whole ethanol concn reached 10% (V/V) in the karusen, the immobilized cell fermentation time can be shortened to about 20 hours by former technology in 72 hours, and throughput can improve more than 3 times.In addition, it is fast that this novel process not only has speed of response, and fermentation period is short, the productivity height, easy to control outside, also have to reduce and pollute, characteristics such as the yeast activity life-span is long.Simultaneously, technology of the present invention can also be used for the transformation to existing traditional zymotic technology, and its smail investments and quick rerurn is effective means of promoting this modern technique of immobilized cell.Technology provided by the invention in addition also can extensively be applied in beer or other fermentation industry.
Claims (3)
1. multi-layer separated biological device, it is characterized in that being equipped with one or more layers particle basket in the fermentor tank of this reactor, the particle basket is a screen basket, put into the fixed yeast gelinite in the screen basket, gelinite can not flow out the particle basket, but fermented liquid can be by the free fluidisation of screen cloth of particle basket, one or several vapor pipes are set in the particle basket, the particle basket is fixed on the vapor pipe, vapor pipe had both played the effect of emission gases, also as the support column of particle basket, the particle basket is placed by support and is fixed in the fermentor tank.
2. according to the described reactor of claim 1, it is characterized in that when adopting the multilayered particles basket, should leaving certain intervals between each layer particle basket, be beneficial to fermented liquid fluidisation in jar.
3. one kind is utilized the described reactor of claim 1 to be used for the production of alcohol with fermented mellow corn using method, it is characterized in that:
(1) preparation of fixed yeast gelinite selects for use polyvinyl alcohol as carrier, and the bacterium number in the fixed yeast gelinite of S. cervisiae after seed culture and Yeast proliferation cultivation is no less than 10
6Individual/ml;
(2) prepared fixed hyperplasia yeast gelinite is put in the particle basket of reactor by 7～20% of fermentor tank volume;
(3) after the treated and saccharification operation of raw material of preparation alcohol, the conversion coefficient of its corn mash should be more than 80%;
(4) its leavening temperature is controlled at 25～40 ℃, and the pH value of fermented liquid is controlled at 3.5～6.0.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN92109880A CN1036146C (en) | 1992-09-09 | 1992-09-09 | Multi-layer separated biological reactor and method of production of alcohol with fermented mellow corn using same |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN92109880A CN1036146C (en) | 1992-09-09 | 1992-09-09 | Multi-layer separated biological reactor and method of production of alcohol with fermented mellow corn using same |
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| Publication Number | Publication Date |
|---|---|
| CN1083861A CN1083861A (en) | 1994-03-16 |
| CN1036146C true CN1036146C (en) | 1997-10-15 |
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| CN92109880A Expired - Fee Related CN1036146C (en) | 1992-09-09 | 1992-09-09 | Multi-layer separated biological reactor and method of production of alcohol with fermented mellow corn using same |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106148172A (en) * | 2015-04-12 | 2016-11-23 | 北京化工大学 | A kind of Substrate Protection device for eliminating bioreactor stirring shearing force in substrate sustained release reaction system |
| CN108660132A (en) * | 2018-04-11 | 2018-10-16 | 南京高新工大生物技术研究院有限公司 | A kind of surface immobilized yeast fermentation technique |
| CN108676788A (en) * | 2018-04-11 | 2018-10-19 | 南京高新工大生物技术研究院有限公司 | A kind of fixed yeast continuously ferments technology |
| CN110117588A (en) * | 2018-12-29 | 2019-08-13 | 南京高新工大生物技术研究院有限公司 | A kind of Novel fermentation tank and its application in ethyl alcohol is prepared in Immobilized yeast |
| CN109988757B (en) * | 2018-12-29 | 2021-04-27 | 江苏省生产力促进中心 | Method for preparing ethanol by semi-continuous fermentation of surface immobilized yeast |
| CN110157600A (en) * | 2019-06-03 | 2019-08-23 | 六安市胜缘食品有限公司 | A kind of chicken claw installation for fermenting |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4209591A (en) * | 1977-08-19 | 1980-06-24 | Stamicarbon, B.V. | Enzymatic conversion process |
| EP0303122A2 (en) * | 1987-08-10 | 1989-02-15 | Kuraray Co., Ltd. | Process for manufacturing spherical hydrated gel containing microorganism immobilized therein |
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1992
- 1992-09-09 CN CN92109880A patent/CN1036146C/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4209591A (en) * | 1977-08-19 | 1980-06-24 | Stamicarbon, B.V. | Enzymatic conversion process |
| EP0303122A2 (en) * | 1987-08-10 | 1989-02-15 | Kuraray Co., Ltd. | Process for manufacturing spherical hydrated gel containing microorganism immobilized therein |
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| CN1083861A (en) | 1994-03-16 |
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