CN114045174A - Liquid microbial improver for soil remediation and preparation method thereof - Google Patents
Liquid microbial improver for soil remediation and preparation method thereof Download PDFInfo
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- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0024—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Glucans; (beta-1,3)-D-Glucans, e.g. paramylon, coriolan, sclerotan, pachyman, callose, scleroglucan, schizophyllan, laminaran, lentinan or curdlan; (beta-1,6)-D-Glucans, e.g. pustulan; (beta-1,4)-D-Glucans; (beta-1,3)(beta-1,4)-D-Glucans, e.g. lichenan; Derivatives thereof
- C08B37/0027—2-Acetamido-2-deoxy-beta-glucans; Derivatives thereof
- C08B37/003—Chitin, i.e. 2-acetamido-2-deoxy-(beta-1,4)-D-glucan or N-acetyl-beta-1,4-D-glucosamine; Chitosan, i.e. deacetylated product of chitin or (beta-1,4)-D-glucosamine; Derivatives thereof
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Abstract
The invention discloses a liquid microbial modifier for soil restoration and a preparation method thereof, wherein the modifier comprises the following raw materials in parts by weight: 5-8 parts of modified resin, 3-5 parts of modified chitosan, 10-15 parts of acetic acid solution, 1-3 parts of photosynthetic bacteria, 1-3 parts of actinomycetes, 1-3 parts of lactic acid bacteria, 1-3 parts of yeast and 10-15 parts of water; in the process of preparing the modifying agent, modified resin is prepared, molecules of the modified resin contain a large number of hydrophilic groups which can absorb water to prevent water loss in soil, and meanwhile, polyglutamic acid structures in the molecules can be degraded by microorganisms to further form nitrogen fertilizer to provide nutrients for plants, and modified chitosan is prepared, after the modified chitosan is absorbed by the plants, the absorption capacity of the plants can be improved, and meanwhile, humic acid structures on the molecules of the modified chitosan can stimulate the growth of the plants.
Description
Technical Field
The invention relates to the technical field of soil remediation, in particular to a liquid microbial improver for soil remediation and a preparation method thereof.
Background
In recent years, along with the construction and development of cities, landscaping construction businesses have been greatly developed, and plant varieties have been increasingly diversified. However, environmental factors have a great influence on plants, and soil, which is an essential substance for plant growth, has a considerable influence on the survival and growth of introduced plants. The soil improvement is a process of adopting corresponding physical, biological or chemical measures aiming at the bad texture and structure of soil, improving the soil property, improving the soil fertility, increasing the crop yield and improving the soil environment for human survival. Materials which are mainly used for improving the physical, chemical and biological properties of soil and are more suitable for plant growth rather than mainly providing plant nutrients are called soil conditioners.
The existing soil conditioner starts from the aspect of improving soil nutrients, the soil conditioner can improve the fertility of soil in a short time, and the nutrient content in the soil is lost along with the loss of water in the soil, so that the normal growth of plants is influenced.
Disclosure of Invention
The invention aims to provide a liquid microbial improver for soil remediation and a preparation method thereof, and solves the problems that the water retention effect of the soil improver is poor and the produced nutrients are easy to run off along with water at the present stage through modified resin and modified chitosan.
The purpose of the invention can be realized by the following technical scheme:
a liquid microbial modifier for soil restoration comprises the following raw materials in parts by weight: 5-8 parts of modified resin, 3-5 parts of modified chitosan, 10-15 parts of acetic acid solution, 1-3 parts of photosynthetic bacteria, 1-3 parts of actinomycetes, 1-3 parts of lactic acid bacteria, 1-3 parts of yeast and 10-15 parts of water;
the modifying agent is prepared by the following steps:
the modifier is prepared by uniformly blending the raw materials at the temperature of 30-35 ℃.
Further, the mass fraction of the acetic acid solution is 1%.
Further, the modified resin is prepared by the following steps:
step A1: after uniformly mixing the p-hydroxyphenylacetaldehyde, the potassium carbonate, the dimethyl sulfate and the acetone, stirring and refluxing for 3-5h at the rotation speed of 150-200r/min and the temperature of 90-100 ℃ to prepare an intermediate 1, uniformly mixing the intermediate 1, the acrylamide and the tetrachloroethane, stirring and adding concentrated hydrochloric acid under the conditions of the rotation speed of 200-300r/min and the temperature of 70-80 ℃ for reaction for 2-3h, filtering to remove filtrate, mixing the filter cake, acrylic acid and sodium hydroxide solution uniformly, adding propenyl polyoxyethylene ether-2000, 2-acrylamide-2-methylbenzenesulfonic acid and potassium persulfate solution, reacting for 2-3h under the conditions that the rotating speed is 200-300r/min and the temperature is 60-70 ℃ to prepare an intermediate 2;
the reaction process is as follows:
step A2: uniformly mixing the intermediate 2, a hydrogen bromide solution and tetrahydrofuran, carrying out reflux reaction for 3-5h at the temperature of 120-130 ℃ to obtain an intermediate 3, uniformly mixing nitroguanidine, diethyl malonate and methanol, refluxing and dropwise adding a sodium methoxide solution at the temperature of 80-90 ℃, reacting for 3-4h, adjusting the pH value of a reaction solution to 5.5-6.5 to obtain an intermediate 4, uniformly mixing the intermediate 4, phosphorus oxychloride and dichloroethane, stirring and adding triethylamine at the rotation speed of 200-300r/min and the temperature of 50-60 ℃, heating to 70-75 ℃, and reacting for 2-3h to obtain an intermediate 5;
the reaction process is as follows:
step A3: uniformly mixing the intermediate 3, the intermediate 5, N-dimethylformamide and sodium hydroxide, reacting for 2-4h at the rotation speed of 150-200r/min and the temperature of 30-50 ℃ to obtain an intermediate 6, dissolving the intermediate 6 in deionized water, adding polyglutamic acid and 1-hydroxybenzotriazole, and reacting for 3-6h at the rotation speed of 200-300r/min and the temperature of 30-40 ℃ to obtain the modified resin.
The reaction process is as follows:
further, the molar ratio of the p-hydroxyphenylacetaldehyde to the dimethyl sulfate in the step A1 is 1:2, the molar ratio of the intermediate 1 to the acrylamide to the tetrachloroethane to the concentrated hydrochloric acid is 0.1mol to 0.2mol to 80mL to 0.5mol, the mass fraction of the concentrated hydrochloric acid is 36%, and the molar ratio of the filter cake to the acrylic acid to the sodium hydroxide solution to the propenyl polyoxyethylene ether-2000 to the 2-acrylamide-2-methylbenzenesulfonic acid is 8g to 5g to 10mL to 5g to 3 g.
Further, the dosage ratio of the intermediate 2 and the hydrogen bromide solution in the step A2 is 0.01mol:50mL, the mass fraction of the hydrogen bromide solution is 35%, the dosage ratio of the nitroguanidine, the diethyl malonate, the methanol and the sodium methoxide solution is 0.1mol:0.12mol:80mL:100mL, and the dosage ratio of the intermediate 4, the phosphorus oxychloride, the dichloroethane and the triethylamine is 0.1mol:0.3mol:60mL:45 mL.
Further, the molar ratio of the intermediate 3, the intermediate 5 and the sodium hydroxide in the step A3 is 2:1:2, and the mass ratio of the intermediate 6 and the polyglutamic acid is 5: 1.
Further, the modified chitosan is prepared by the following steps:
step B1: uniformly mixing chitosan, deionized water and acetic acid, stirring and adding a maleic anhydride acetone solution under the conditions that the rotating speed is 200-300r/min and the temperature is 25-30 ℃, reacting for 5-6h, and drying to obtain pre-modified chitosan;
step B2: uniformly mixing humic acid, deionized water and potassium hydroxide, stirring and adding sulfur trioxide under the conditions that the rotating speed is 150-200r/min and the temperature is 60-70 ℃, reacting for 2-3h, and adjusting the pH value of the reaction to 3-5 to prepare sulfonated humic acid;
step B3: uniformly mixing sulfonated humic acid, deionized water and acetic acid, adding pre-modified chitosan, dicyclohexylcarbodiimide and 4-dimethylaminopyridine, and carrying out reflux reaction for 3-5h at the rotation speed of 200-300r/min and the temperature of 110-120 ℃ to obtain the modified chitosan.
Furthermore, the dosage ratio of the chitosan, the deionized water, the acetic acid and the maleic anhydride in the step B1 is 0.06g:50mL:0.5mL:0.03 g.
Furthermore, the dosage ratio of the humic acid, the deionized water, the potassium hydroxide and the sulfur trioxide in the step B2 is 0.9g to 10mL to 0.2g to 0.3 g.
Furthermore, the dosage ratio of the sulfonated humic acid, the deionized water, the acetic acid, the pre-modified chitosan, the dicyclohexylcarbodiimide and the 4-dimethylaminopyridine in the step B3 is 0.03g to 10mL to 1mL to 0.02g to 0.01 g.
The invention has the beneficial effects that: the invention prepares modified resin in the process of preparing a liquid microorganism modifier for soil restoration, the modified resin takes p-hydroxyphenylacetaldehyde as a raw material and carries out phenolic hydroxyl protection by dimethyl sulfate to prepare an intermediate 1, the intermediate 1 reacts with acrylamide under an acidic condition, then the intermediate 1 is copolymerized with acrylic acid, propenyl polyoxyethylene ether-2000, 2-acrylamide-2-methyl benzenesulfonic acid to prepare an intermediate 2, the intermediate 2 is deprotected to prepare an intermediate 3, nitroguanidine reacts with diethyl malonate to prepare an intermediate 4, the intermediate 4 is treated with phosphorus oxychloride to prepare an intermediate 5, the intermediate 3 reacts with the intermediate 5 to enable phenolic hydroxyl on the intermediate 3 to react with chlorine atom sites on the intermediate 5 to prepare an intermediate 6, and the intermediate 6 reacts with polyglutamic acid, the amino on the intermediate 6 and the carboxyl on the polyglutamic acid are subjected to dehydration condensation to prepare modified resin, the molecules of the modified resin contain a large number of hydrophilic groups which can absorb water and prevent the water in soil from losing, meanwhile, the polyglutamic acid structure in the molecule can be degraded by microorganisms, so that a nitrogen fertilizer is formed to provide nutrients for plants, and the modified chitosan is prepared, the modified chitosan is prepared by reacting chitosan serving as a raw material with maleic anhydride to open a ring of the maleic anhydride, reacting carboxyl after the ring opening with amino on the chitosan to prepare pre-modified chitosan, sulfonating humic acid to prepare sulfonated humic acid, condensing the pre-modified chitosan with hydroxyl on the sulfonated humic acid to prepare modified chitosan, after the modified chitosan is absorbed by plants, the absorption capacity of the plants can be improved, and the humic acid structure on the modified chitosan molecules can stimulate the growth of the plants.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A liquid microbial modifier for soil restoration comprises the following raw materials in parts by weight: 5 parts of modified resin, 3 parts of modified chitosan, 10 parts of acetic acid solution, 1 part of photosynthetic bacteria, 1 part of actinomycetes, 1 part of lactic acid bacteria, 1 part of saccharomycetes and 10 parts of water;
the modifying agent is prepared by the following steps:
the raw materials are evenly blended at the temperature of 30 ℃ to prepare the modifier.
The modified resin is prepared by the following steps:
step A1: after uniformly mixing p-hydroxyphenylacetaldehyde, potassium carbonate, dimethyl sulfate and acetone, stirring and refluxing for 3h at the rotation speed of 150r/min and the temperature of 90 ℃ to prepare an intermediate 1, uniformly mixing the intermediate 1, acrylamide and tetrachloroethane, stirring and adding concentrated hydrochloric acid at the rotation speed of 200r/min and the temperature of 70 ℃ to react for 2h, filtering to remove filtrate, uniformly mixing a filter cake, acrylic acid and a sodium hydroxide solution, adding propenyl polyoxyethylene ether-2000, 2-acrylamide-2-methylbenzenesulfonic acid and a potassium persulfate solution, and reacting for 2h at the rotation speed of 200r/min and the temperature of 60 ℃ to prepare an intermediate 2;
step A2: uniformly mixing the intermediate 2, a hydrogen bromide solution and tetrahydrofuran, carrying out reflux reaction for 3 hours at the temperature of 120 ℃ to obtain an intermediate 3, uniformly mixing nitroguanidine, diethyl malonate and methanol, refluxing and dropwise adding a sodium methoxide solution at the temperature of 80 ℃, regulating the pH value of a reaction solution to 5.5 after carrying out reaction for 3 hours to obtain an intermediate 4, uniformly mixing the intermediate 4, phosphorus oxychloride and dichloroethane, stirring at the rotation speed of 200r/min and at the temperature of 50 ℃, adding triethylamine, heating to the temperature of 70 ℃, and carrying out reaction for 2 hours to obtain an intermediate 5;
step A3: uniformly mixing the intermediate 3, the intermediate 5, N-dimethylformamide and sodium hydroxide, reacting for 2 hours at the rotation speed of 150r/min and the temperature of 30 ℃ to obtain an intermediate 6, dissolving the intermediate 6 in deionized water, adding polyglutamic acid and 1-hydroxybenzotriazole, and reacting for 3 hours at the rotation speed of 200r/min and the temperature of 30 ℃ to obtain the modified resin.
The modified chitosan is prepared by the following steps:
step B1: uniformly mixing chitosan, deionized water and acetic acid, stirring and adding a maleic anhydride acetone solution under the conditions that the rotating speed is 200r/min and the temperature is 25 ℃, reacting for 5 hours, and drying to obtain pre-modified chitosan;
step B2: uniformly mixing humic acid, deionized water and potassium hydroxide, stirring and adding sulfur trioxide under the conditions that the rotating speed is 150r/min and the temperature is 60 ℃, reacting for 2 hours, and adjusting the pH value of the reaction to be 3 to prepare sulfonated humic acid;
step B3: uniformly mixing sulfonated humic acid, deionized water and acetic acid, adding pre-modified chitosan, dicyclohexylcarbodiimide and 4-dimethylaminopyridine, and carrying out reflux reaction for 3h at the rotation speed of 200r/min and the temperature of 110 ℃ to obtain the modified chitosan.
Example 2
A liquid microbial modifier for soil restoration comprises the following raw materials in parts by weight: 6 parts of modified resin, 4 parts of modified chitosan, 13 parts of acetic acid solution, 2 parts of photosynthetic bacteria, 2 parts of actinomycetes, 2 parts of lactic acid bacteria, 2 parts of saccharomycetes and 13 parts of water;
the modifying agent is prepared by the following steps:
the raw materials are evenly blended at the temperature of 33 ℃ to prepare the modifier.
The modified resin is prepared by the following steps:
step A1: after uniformly mixing p-hydroxyphenylacetaldehyde, potassium carbonate, dimethyl sulfate and acetone, stirring and refluxing for 4 hours at the conditions of the rotating speed of 180r/min and the temperature of 95 ℃ to prepare an intermediate 1, uniformly mixing the intermediate 1, acrylamide and tetrachloroethane, stirring and adding concentrated hydrochloric acid at the rotating speed of 200r/min and the temperature of 75 ℃ to react for 2.5 hours, filtering to remove filtrate, uniformly mixing a filter cake, acrylic acid and a sodium hydroxide solution, adding propenyl polyoxyethylene ether-2000, 2-acrylamide-2-methylbenzenesulfonic acid and a potassium persulfate solution, and reacting for 2.5 hours at the rotating speed of 300r/min and the temperature of 65 ℃ to prepare an intermediate 2;
step A2: uniformly mixing the intermediate 2, a hydrogen bromide solution and tetrahydrofuran, carrying out reflux reaction for 4 hours at the temperature of 125 ℃ to obtain an intermediate 3, uniformly mixing nitroguanidine, diethyl malonate and methanol, refluxing and dropwise adding a sodium methoxide solution at the temperature of 85 ℃, reacting for 3.5 hours, adjusting the pH value of a reaction solution to 6 to obtain an intermediate 4, uniformly mixing the intermediate 4, phosphorus oxychloride and dichloroethane, stirring at the rotation speed of 200r/min and at the temperature of 55 ℃, adding triethylamine, heating to the temperature of 73 ℃, reacting for 2.5 hours to obtain an intermediate 5;
step A3: uniformly mixing the intermediate 3, the intermediate 5, N-dimethylformamide and sodium hydroxide, reacting for 3 hours at the rotation speed of 180r/min and the temperature of 40 ℃ to obtain an intermediate 6, dissolving the intermediate 6 in deionized water, adding polyglutamic acid and 1-hydroxybenzotriazole, and reacting for 5 hours at the rotation speed of 200r/min and the temperature of 35 ℃ to obtain the modified resin.
The modified chitosan is prepared by the following steps:
step B1: uniformly mixing chitosan, deionized water and acetic acid, stirring and adding a maleic anhydride acetone solution under the conditions that the rotating speed is 300r/min and the temperature is 28 ℃, reacting for 5.5 hours, and drying to obtain pre-modified chitosan;
step B2: uniformly mixing humic acid, deionized water and potassium hydroxide, stirring and adding sulfur trioxide under the conditions that the rotating speed is 180r/min and the temperature is 65 ℃, reacting for 2.3 hours, and adjusting the pH value of the reaction to be 4 to prepare sulfonated humic acid;
step B3: uniformly mixing sulfonated humic acid, deionized water and acetic acid, adding pre-modified chitosan, dicyclohexylcarbodiimide and 4-dimethylaminopyridine, and carrying out reflux reaction for 4 hours at the rotation speed of 300r/min and the temperature of 115 ℃ to obtain the modified chitosan.
Example 3
A liquid microbial modifier for soil restoration comprises the following raw materials in parts by weight: 8 parts of modified resin, 5 parts of modified chitosan, 15 parts of acetic acid solution, 3 parts of photosynthetic bacteria, 3 parts of actinomycetes, 3 parts of lactic acid bacteria, 3 parts of saccharomycetes and 15 parts of water;
the modifying agent is prepared by the following steps:
the raw materials are evenly blended at the temperature of 35 ℃ to prepare the modifier.
The modified resin is prepared by the following steps:
step A1: after uniformly mixing p-hydroxyphenylacetaldehyde, potassium carbonate, dimethyl sulfate and acetone, stirring and refluxing for 5 hours at the rotation speed of 200r/min and the temperature of 100 ℃ to prepare an intermediate 1, uniformly mixing the intermediate 1, acrylamide and tetrachloroethane, stirring and adding concentrated hydrochloric acid at the rotation speed of 300r/min and the temperature of 80 ℃ to react for 3 hours, filtering and removing filtrate, uniformly mixing a filter cake, acrylic acid and a sodium hydroxide solution, adding propenyl polyoxyethylene ether-2000, 2-acrylamide-2-methylbenzenesulfonic acid and a potassium persulfate solution, and reacting for 3 hours at the rotation speed of 300r/min and the temperature of 70 ℃ to prepare an intermediate 2;
step A2: uniformly mixing the intermediate 2, a hydrogen bromide solution and tetrahydrofuran, carrying out reflux reaction for 5 hours at the temperature of 130 ℃ to obtain an intermediate 3, uniformly mixing nitroguanidine, diethyl malonate and methanol, refluxing and dropwise adding a sodium methoxide solution at the temperature of 90 ℃, regulating the pH value of a reaction solution to 6.5 after carrying out reaction for 4 hours to obtain an intermediate 4, uniformly mixing the intermediate 4, phosphorus oxychloride and dichloroethane, stirring at the rotation speed of 300r/min and at the temperature of 60 ℃, adding triethylamine, heating to the temperature of 75 ℃, and carrying out reaction for 3 hours to obtain an intermediate 5;
step A3: uniformly mixing the intermediate 3, the intermediate 5, N-dimethylformamide and sodium hydroxide, reacting for 4 hours at the rotation speed of 200r/min and the temperature of 50 ℃ to obtain an intermediate 6, dissolving the intermediate 6 in deionized water, adding polyglutamic acid and 1-hydroxybenzotriazole, and reacting for 6 hours at the rotation speed of 300r/min and the temperature of 40 ℃ to obtain the modified resin.
The modified chitosan is prepared by the following steps:
step B1: uniformly mixing chitosan, deionized water and acetic acid, stirring and adding a maleic anhydride acetone solution under the conditions that the rotating speed is 300r/min and the temperature is 30 ℃, reacting for 6 hours, and drying to obtain pre-modified chitosan;
step B2: uniformly mixing humic acid, deionized water and potassium hydroxide, stirring and adding sulfur trioxide under the conditions that the rotating speed is 200r/min and the temperature is 70 ℃, reacting for 3 hours, and adjusting the pH value of the reaction to be 5 to prepare sulfonated humic acid;
step B3: uniformly mixing sulfonated humic acid, deionized water and acetic acid, adding pre-modified chitosan, dicyclohexylcarbodiimide and 4-dimethylaminopyridine, and carrying out reflux reaction for 5 hours at the rotation speed of 300r/min and the temperature of 120 ℃ to obtain the modified chitosan.
Comparative example 1
This comparative example compares with example 1 without adding a modified resin and the rest of the procedure is the same.
Comparative example 2
The comparative example is a soil conditioner disclosed in Chinese patent CN 110330977A.
Comparative example 3
The comparative example is a soil conditioner disclosed in Chinese patent CN 110079332A.
Under different moisture conditions that the soil reaches a saturated state, the soil conditioners prepared in examples 1-3 and comparative examples 1-3 are respectively added, and the time required for the moisture in the soil to completely evaporate is detected, and the results are shown in the following table;
as can be seen from the above table, the water retention capacity of the conditioner prepared in examples 1 to 3 is 10 to 11 days in soil with a water content of 30%, 17 to 18 days in soil with a water content of 50%, and 25 to 26 days in soil with a water content of 70%, indicating that the conditioner of the present invention has a good water retention effect.
The foregoing is merely exemplary and illustrative of the principles of the present invention and various modifications, additions and substitutions of the specific embodiments described herein may be made by those skilled in the art without departing from the principles of the present invention or exceeding the scope of the claims set forth herein.
Claims (5)
1. A liquid microorganism modifying agent for soil remediation is characterized in that: the feed comprises the following raw materials in parts by weight: 5-8 parts of modified resin, 3-5 parts of modified chitosan, 10-15 parts of acetic acid solution, 1-3 parts of photosynthetic bacteria, 1-3 parts of actinomycetes, 1-3 parts of lactic acid bacteria, 1-3 parts of yeast and 10-15 parts of water;
the modified resin is prepared by the following steps:
step A1: uniformly mixing p-hydroxyphenylacetaldehyde, potassium carbonate, dimethyl sulfate and acetone, stirring and refluxing to prepare an intermediate 1, uniformly mixing the intermediate 1, acrylamide and tetrachloroethane, stirring and adding concentrated hydrochloric acid, reacting, filtering to remove filtrate, uniformly mixing a filter cake, acrylic acid and a sodium hydroxide solution, adding propenyl polyoxyethylene ether-2000, 2-acrylamide-2-methylbenzenesulfonic acid and a potassium persulfate solution, and reacting to prepare an intermediate 2;
step A2: uniformly mixing the intermediate 2, a hydrogen bromide solution and tetrahydrofuran, performing reflux reaction to obtain an intermediate 3, uniformly mixing nitroguanidine, diethyl malonate and methanol, refluxing, dropwise adding a sodium methoxide solution, reacting, adjusting the pH value of a reaction solution to obtain an intermediate 4, uniformly mixing the intermediate 4, phosphorus oxychloride and dichloroethane, stirring, adding triethylamine, and heating to react to obtain an intermediate 5;
step A3: mixing the intermediate 3, the intermediate 5, N-dimethylformamide and sodium hydroxide for reaction to prepare an intermediate 6, dissolving the intermediate 6 in deionized water, adding polyglutamic acid and 1-hydroxybenzotriazole for reaction to prepare the modified resin.
2. A liquid microbial amendment for soil remediation according to claim 1 wherein: the molar ratio of the p-hydroxyphenylacetaldehyde to the dimethyl sulfate in the step A1 is 1:2, the molar ratio of the intermediate 1 to the acrylamide to the tetrachloroethane to the concentrated hydrochloric acid is 0.1mol:0.2mol:80mL:0.5mol, the mass fraction of the concentrated hydrochloric acid is 36%, and the molar ratio of the filter cake to the acrylic acid to the sodium hydroxide solution to the propenyl polyoxyethylene ether-2000 to the 2-acrylamide-2-methylbenzenesulfonic acid is 8g:5g:10mL:5g:3 g.
3. A liquid microbial amendment for soil remediation according to claim 1 wherein: the dosage ratio of the intermediate 2 and the hydrogen bromide solution in the step A2 is 0.01mol:50mL, the mass fraction of the hydrogen bromide solution is 35%, the dosage ratio of the nitroguanidine, the diethyl malonate, the methanol and the sodium methoxide solution is 0.1mol:0.12mol:80mL:100mL, and the dosage ratio of the intermediate 4, the phosphorus oxychloride, the dichloroethane and the triethylamine is 0.1mol:0.3mol:60mL:45 mL.
4. A liquid microbial amendment for soil remediation according to claim 1 wherein: the molar ratio of the intermediate 3 to the intermediate 5 to the sodium hydroxide in the step A3 is 2:1:2, and the mass ratio of the intermediate 6 to the polyglutamic acid is 5: 1.
5. The method for preparing a liquid microbial improver for soil remediation according to claim 1, wherein: the method specifically comprises the following steps:
weighing modified resin, modified chitosan, acetic acid solution, photosynthetic bacteria, actinomycetes, lactic acid bacteria, yeast and water, and blending uniformly at 30-35 deg.C to obtain the modifier.
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| CN114456008A (en) * | 2022-02-25 | 2022-05-10 | 峰景园林工程集团有限公司 | Modifying agent for improving tree transplanting survival rate and preparation method thereof |
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