CN114034855A - Protein analysis reaction disc - Google Patents
Protein analysis reaction disc Download PDFInfo
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- CN114034855A CN114034855A CN202111296948.2A CN202111296948A CN114034855A CN 114034855 A CN114034855 A CN 114034855A CN 202111296948 A CN202111296948 A CN 202111296948A CN 114034855 A CN114034855 A CN 114034855A
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- reaction
- slot
- dish
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- protein analysis
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- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 20
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 20
- 238000004458 analytical method Methods 0.000 title claims abstract description 19
- 238000001514 detection method Methods 0.000 claims abstract description 26
- 230000001105 regulatory effect Effects 0.000 claims description 6
- 230000005540 biological transmission Effects 0.000 claims description 5
- 230000037431 insertion Effects 0.000 claims 4
- 238000003780 insertion Methods 0.000 claims 4
- 238000000926 separation method Methods 0.000 abstract description 3
- 210000001503 joint Anatomy 0.000 abstract 1
- 238000002835 absorbance Methods 0.000 description 2
- 230000001174 ascending effect Effects 0.000 description 2
- 230000005489 elastic deformation Effects 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000000149 penetrating effect Effects 0.000 description 2
- 238000003491 array Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000002331 protein detection Methods 0.000 description 1
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
- G01N33/5304—Reaction vessels, e.g. agglutination plates
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- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides a protein analysis reaction dish, includes detection module, reaction dish and rotatable reaction dish, the up end of reaction dish is equipped with a plurality of slots of arranging along circumference, the reaction dish peg graft in the slot, every all be equipped with in the slot and be used for butt joint to the elasticity buckle of reaction dish, every the lateral wall of slot all is equipped with and runs through to the observation hole of the lateral wall of reaction dish, detection module includes at least one of transmission-type light detector and scattering formula light detector, detection module is just right observe the hole setting. According to the scheme, the reaction disc is provided with the slot and the elastic buckle, so that the reaction vessel can be pulled out from the slot to realize separation when needed, and the replacement is simple and convenient; meanwhile, due to the arrangement of the observation holes, the light loss of the reaction vessels is effectively reduced, and the mutual interference between the adjacent reaction vessels is avoided, so that the detection assembly can more accurately detect the sample in the reaction vessel through the observation holes.
Description
Technical Field
The invention relates to the technical field of cell protein analysis, in particular to a protein analysis reaction disc.
Background
The detection and analysis of specific protein has important clinical significance for disease diagnosis, and with the development of protein detection technology and the expansion of clinical application range in recent years, an immune turbidimetric analyzer gradually becomes clinical examination equipment which is commonly purchased in domestic laboratories. In such analyzers, a sample and a reagent are often added to a reaction cuvette on a rotatable reaction vessel through a sample application needle, and then absorbance of a reaction solution in the reaction cuvette is detected and analyzed by a photodetector.
However, in practical use, the reaction vessels on the reaction vessel are often arranged in a plurality of and dense arrays, and when absorbance detection is performed on the reaction vessels, adjacent reaction vessels may interfere with each other, resulting in a large error of the detection result.
Disclosure of Invention
The invention solves the problems that: provides a protein analysis reaction disc capable of realizing accurate detection of specific protein.
In order to solve the above problems, the present invention provides a protein analysis reaction tray, which includes a detection assembly, a reaction cuvette and a rotatable reaction tray, wherein the upper end surface of the reaction tray is provided with a plurality of slots arranged along the circumferential direction, the reaction cuvette is inserted into the slots, each slot is provided with an elastic buckle for abutting against the reaction cuvette, the side wall of each slot is provided with an observation hole penetrating to the outer side wall of the reaction tray, the detection assembly includes at least one of a transmission type light detector and a scattering type light detector, and the detection assembly is arranged opposite to the observation hole.
Compared with the prior art, the reaction dish is provided with the slot and the elastic buckle, so that the reaction dish can be pulled out from the slot to realize separation when needed, and the replacement is simple and convenient; meanwhile, due to the arrangement of the observation holes, the light loss of the reaction vessels is effectively reduced, and the mutual interference between the adjacent reaction vessels is avoided, so that the detection assembly can more accurately detect the sample in the reaction vessel through the observation holes.
Preferably, the upper end face of the reaction disc is provided with a plurality of through grooves distributed along the circumferential direction, the through grooves are radially arranged and correspond to the slots one by one, the through grooves penetrate between the slot walls of the slots and the outer side wall of the reaction disc, and the elastic buckles are radially arranged along the reaction disc. The setting that leads to the groove makes the reaction dish can follow circumference suitably elastic deformation, and the elasticity buckle has then realized the reaction ware at ascending elasticity butt in radial to guarantee that the connection between reaction ware and the reaction dish is more firm, and the dismouting is more convenient.
Preferably, be equipped with the holding tank on the support frame, the reaction disc be the annular and rotate connect in the inside of holding tank, the lateral wall detachable connection of holding tank has the mount pad, detection subassembly connect in the mount pad. After adopting this structure, the holding tank can form comparatively stable cultivation environment for the reaction disc, and the setting up of mount pad makes things convenient for the installation and the adjustment of determine module.
Preferably, the mounting seat includes a first base and a second base arranged along the circumferential direction of the accommodating groove, the detection module includes two scattering photodetectors and a transmission photodetector, the two scattering photodetectors are respectively connected to the upper portion and the lower portion of the first base, a light path is arranged on one side of the first base facing the observation hole, and the light path is communicated to the two scattering photodetectors; the transmission-type light detector is connected to the second base and faces the observation hole, so that the reaction vessel can be detected more accurately.
Preferably, the slot wall of each slot is provided with two caulking grooves which are arranged along the radial direction, the two caulking grooves are respectively positioned at two sides of the through slot corresponding to the same slot, each caulking groove is provided with an elastic buckle correspondingly, one side of each elastic buckle is clamped in the caulking groove, and the other side of each elastic buckle is abutted to the reaction vessel, so that the reaction vessel is better fixed.
Preferably, the reaction vessel further comprises an adjusting bolt, the embedded groove penetrates through the upper end face of the reaction disc, and the adjusting bolt is inserted between the embedded groove and the elastic buckle, so that the elastic abutting degree between the elastic buckle and the reaction vessel can be conveniently adjusted.
As preferred, still be equipped with temperature regulation regulating assembly and humidity control subassembly on the support frame, temperature regulating assembly is used for adjusting temperature in the holding tank, humidity control subassembly is used for adjusting humidity in the holding tank to build stable cultivation reaction environment for the reaction disc in the holding tank.
Drawings
FIG. 1 is a schematic axial view of a reaction disk for protein analysis;
FIG. 2 is a schematic top view of a protein analysis reaction tray;
FIG. 3 is an enlarged view of a portion of area A of FIG. 1;
FIG. 4 is an enlarged view of the area A of FIG. 1 with the support frame removed;
FIG. 5 is an enlarged partial view of the area B in FIG. 2;
FIG. 6 is an enlarged partial view of region C of FIG. 5;
FIG. 7 is a schematic cross-sectional view of a second base of the protein analysis reaction plate.
Description of reference numerals:
a 1-reaction disk; a 11-reaction dish; a 12-slot; a 13-viewport; a 14-through slot; a 15-caulking groove; a 2-support; a 21-holding tank; a 31-first base; a 32-second base; a 33-track; a 4-elastic snap; a 41-adjusting bolt.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below. The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention. It should be noted that all the directional indicators (such as up, down, left, right, front, back, inside, and outside) in the embodiments of the present invention are only used to explain the relative position relationship between the components, the motion situation, and the like in a specific posture (as shown in the drawings), and if the specific posture is changed, the directional indicator is changed accordingly.
Referring to fig. 1 to 7, a protein analysis reaction tray provided by an embodiment of the present invention includes a detection assembly, a reaction tray a11, and a rotatable reaction tray a1, an upper end surface of the reaction tray a1 is provided with a plurality of slots a12 arranged along a circumferential direction, the reaction tray a11 is inserted into the slots a12, each slot a12 is provided with an elastic buckle a4 for abutting against the reaction tray a11, a side wall of each slot a12 is provided with an observation hole a13 penetrating through an outer side wall of the reaction tray a1, the detection assembly includes at least one of a transmission type photodetector and a scattering type photodetector, and the detection assembly is disposed opposite to the observation hole a 13.
According to the scheme, the reaction disk a1 is provided with the slot a12 and the elastic buckle a4, so that the reaction dish a11 can be pulled out of the slot a12 to realize separation when needed, and replacement is simple and convenient; meanwhile, due to the arrangement of the observation hole a13, the light loss of the reaction cuvette a11 is effectively reduced, and the mutual interference between the adjacent reaction cuvettes a11 is avoided, so that the detection assembly can more accurately detect the sample in the reaction cuvette a11 through the observation hole a 13.
In this embodiment, the upper end surface of the reaction disk a1 is provided with a plurality of through slots a14 arranged along the circumferential direction, the through slots a14 are arranged along the radial direction and correspond to the slots a12 one by one, the through slots a14 penetrate between the slot wall of the slot a12 and the outer side wall of the reaction disk a1, and the elastic buckles a4 are arranged along the radial direction of the reaction disk a 1. The setting that leads to groove a14 makes reaction dish a1 can follow circumference appropriate elastic deformation, and elasticity buckle a4 then has realized reacting dish a11 at radial ascending elasticity butt to guarantee that the connection between reaction dish a11 and the reaction dish a1 is more firm, and the dismouting is more convenient.
As the expansion to the above embodiment, still include support frame a2, be equipped with holding tank a21 on support frame a2, reaction disc a1 is annular and rotates and connect in the inside of holding tank a21, and the lateral wall detachable connection of holding tank a21 has the mount pad, and detection module connects in the mount pad. After adopting this structure, holding tank a21 can form comparatively stable cultivation environment for reaction dish a1, and the installation and the adjustment of the convenient determine module of setting up of mount pad.
The mounting seat comprises a first base a31 and a second base a32 which are arranged along the circumferential direction of the accommodating groove a21, the detection module comprises two scattering photodetectors and a transmission photodetector, the two scattering photodetectors are respectively connected to the upper part and the lower part of the first base a31, a light channel a33 is arranged on one side, facing the observation hole a13, of the first base a31, and the light channel a33 is communicated with the two scattering photodetectors; the transmissive light detector is connected to the second base a32 and disposed toward the viewing hole a13, thereby achieving more accurate detection of the reaction cuvette a 11.
Furthermore, the groove wall of each slot a12 is provided with two caulking grooves a15 arranged along the radial direction, the two caulking grooves a15 are respectively located at two sides of the through groove a14 corresponding to the same slot a12, each caulking groove a15 is correspondingly provided with an elastic buckle a4, one side of the elastic buckle a4 is clamped in the caulking groove a15, and the other side of the elastic buckle a4 is abutted against the reaction vessel a11, so that the reaction vessel a11 is better fixed.
Further, the reaction device also comprises an adjusting bolt a41, wherein the embedded groove a15 penetrates the upper end face of the reaction disk a1, and the adjusting bolt a41 is used for being plugged between the embedded groove a15 and the elastic buckle a4, so that the elastic abutting degree between the elastic buckle a4 and the reaction disk a11 can be conveniently adjusted.
In addition, a temperature adjusting and adjusting assembly (not shown) for adjusting the temperature in the holding tank a21 and a humidity adjusting assembly (not shown) for adjusting the humidity in the holding tank a21 are further provided on the supporting frame a2, so as to create a stable culture reaction environment for the reaction disk a1 in the holding tank a 21.
Although the present disclosure has been described above, the scope of the present disclosure is not limited thereto. It will be apparent to those skilled in the art that various changes and modifications can be made without departing from the spirit and scope of the disclosure, and these changes and modifications will fall within the scope of the invention.
Claims (7)
1. The protein analysis reaction disc is characterized by comprising a detection assembly, a reaction dish (a11) and a rotatable reaction dish (a1), wherein the upper end surface of the reaction dish (a1) is provided with a plurality of slots (a12) which are circumferentially arranged, the reaction dish (a11) is inserted into the slot (a12), each slot (a12) is internally provided with an elastic buckle (a4) which is used for abutting against the reaction dish (a11), the side wall of each slot (a12) is provided with an observation hole (a13) which penetrates through the outer side wall of the reaction dish (a1), the detection assembly comprises at least one of a transmission type light detector and a scattering type light detector, and the detection assembly is arranged right opposite to the observation hole (a 13).
2. The protein analysis reaction disc according to claim 1, wherein the upper end surface of the reaction disc (a1) is provided with a plurality of circumferentially arranged through slots (a14), the through slots (a14) are radially arranged and correspond to the insertion slots (a12) one by one, the through slots (a14) penetrate between the slot wall of the insertion slot (a12) and the outer side wall of the reaction disc (a1), and the elastic buckles (a4) are arranged along the radial direction of the reaction disc (a 1).
3. The protein analysis reaction disc of claim 2, further comprising a supporting frame (a2), wherein the supporting frame (a2) is provided with a receiving groove (a21), the reaction disc (a1) is annular and is rotatably connected to the inside of the receiving groove (a21), the side wall of the receiving groove (a21) is detachably connected with a mounting seat, and the detection assembly is connected to the mounting seat.
4. The protein analysis reaction disk according to claim 3, wherein the mounting seat comprises a first base (a31) and a second base (a32) arranged along a circumferential direction of the receiving groove (a21), the detection module comprises two scattered light detectors and one transmitted light detector, the two scattered light detectors are respectively connected to an upper portion and a lower portion of the first base (a31), a light path (a33) is provided on a side of the first base (a31) facing the observation hole (a13), and the light path (a33) is communicated to the two scattered light detectors; the transmissive light detector is connected to the second base (a32) and disposed toward the viewing aperture (a 13).
5. The protein analysis reaction disc of claim 4, wherein the slot wall of each slot (a12) is provided with two radially arranged slots (a15), the two slots (a15) are respectively located at two sides of the through slot (a14) corresponding to the same slot (a12), each slot (a15) is corresponding to an elastic buckle (a4), one side of the elastic buckle (a4) is clamped in the slot (a15), and the other side of the elastic buckle abuts against the reaction vessel (a 11).
6. The protein analysis reaction plate according to claim 5, further comprising a regulation pin (a41), wherein the insertion groove (a15) penetrates to the upper end face of the reaction plate (a1), and the regulation pin (a41) is used for being inserted between the insertion groove (a15) and the elastic buckle (a 4).
7. The protein analysis reaction plate according to any one of claims 3 to 6, wherein the supporting frame (a2) is further provided with a temperature regulating assembly for regulating the temperature in the holding tank (a21) and a humidity regulating assembly for regulating the humidity in the holding tank (a 21).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111296948.2A CN114034855A (en) | 2021-10-29 | 2021-10-29 | Protein analysis reaction disc |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111296948.2A CN114034855A (en) | 2021-10-29 | 2021-10-29 | Protein analysis reaction disc |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114034855A true CN114034855A (en) | 2022-02-11 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111296948.2A Pending CN114034855A (en) | 2021-10-29 | 2021-10-29 | Protein analysis reaction disc |
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| CN (1) | CN114034855A (en) |
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| CN112798775A (en) * | 2021-01-13 | 2021-05-14 | 烟台信达威医疗科技有限公司 | Dark box detection system of chemiluminescence immunoassay analyzer |
| CN213348948U (en) * | 2020-08-14 | 2021-06-04 | 广州万孚生物技术股份有限公司 | Reaction cup fixing mechanism and reaction cup holder |
-
2021
- 2021-10-29 CN CN202111296948.2A patent/CN114034855A/en active Pending
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