CN114032204B - Composite microbial agent for treating offshore oil sewage - Google Patents
Composite microbial agent for treating offshore oil sewage Download PDFInfo
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- CN114032204B CN114032204B CN202111608775.3A CN202111608775A CN114032204B CN 114032204 B CN114032204 B CN 114032204B CN 202111608775 A CN202111608775 A CN 202111608775A CN 114032204 B CN114032204 B CN 114032204B
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- 239000002131 composite material Substances 0.000 title claims abstract description 38
- 239000010865 sewage Substances 0.000 title claims abstract description 27
- 230000000813 microbial effect Effects 0.000 title claims abstract description 11
- 230000001580 bacterial effect Effects 0.000 claims abstract description 45
- 239000003208 petroleum Substances 0.000 claims abstract description 26
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 21
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 21
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 18
- 239000007787 solid Substances 0.000 claims abstract description 17
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 14
- 150000003839 salts Chemical class 0.000 claims abstract description 10
- 230000033558 biomineral tissue development Effects 0.000 claims abstract description 6
- 239000007788 liquid Substances 0.000 claims description 12
- 241000894006 Bacteria Species 0.000 claims description 11
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 10
- LQZZUXJYWNFBMV-UHFFFAOYSA-N dodecan-1-ol Chemical compound CCCCCCCCCCCCO LQZZUXJYWNFBMV-UHFFFAOYSA-N 0.000 claims description 10
- 239000010802 sludge Substances 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 9
- 239000002351 wastewater Substances 0.000 claims description 7
- 229920000168 Microcrystalline cellulose Polymers 0.000 claims description 5
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 5
- 235000019813 microcrystalline cellulose Nutrition 0.000 claims description 5
- 239000008108 microcrystalline cellulose Substances 0.000 claims description 5
- 229940016286 microcrystalline cellulose Drugs 0.000 claims description 5
- -1 polyoxyethylene Polymers 0.000 claims description 5
- DAJSVUQLFFJUSX-UHFFFAOYSA-M sodium;dodecane-1-sulfonate Chemical compound [Na+].CCCCCCCCCCCCS([O-])(=O)=O DAJSVUQLFFJUSX-UHFFFAOYSA-M 0.000 claims description 5
- 229920000058 polyacrylate Polymers 0.000 claims description 3
- NNMHYFLPFNGQFZ-UHFFFAOYSA-M sodium polyacrylate Polymers [Na+].[O-]C(=O)C=C NNMHYFLPFNGQFZ-UHFFFAOYSA-M 0.000 claims description 2
- 241000186660 Lactobacillus Species 0.000 claims 1
- 229940039696 lactobacillus Drugs 0.000 claims 1
- 240000006024 Lactobacillus plantarum Species 0.000 abstract description 22
- 235000013965 Lactobacillus plantarum Nutrition 0.000 abstract description 22
- 229940072205 lactobacillus plantarum Drugs 0.000 abstract description 22
- 230000015556 catabolic process Effects 0.000 abstract description 12
- 238000006731 degradation reaction Methods 0.000 abstract description 12
- 230000000694 effects Effects 0.000 abstract description 9
- 230000008485 antagonism Effects 0.000 abstract description 5
- 230000015784 hyperosmotic salinity response Effects 0.000 abstract description 4
- 239000002054 inoculum Substances 0.000 abstract description 3
- 239000003209 petroleum derivative Substances 0.000 abstract description 3
- 238000002360 preparation method Methods 0.000 description 13
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- 239000002283 diesel fuel Substances 0.000 description 5
- 239000002245 particle Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 238000012258 culturing Methods 0.000 description 3
- 239000003995 emulsifying agent Substances 0.000 description 3
- 229930195733 hydrocarbon Natural products 0.000 description 3
- 150000002430 hydrocarbons Chemical class 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 239000001110 calcium chloride Substances 0.000 description 2
- 229910001628 calcium chloride Inorganic materials 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000000945 filler Substances 0.000 description 2
- 238000009630 liquid culture Methods 0.000 description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 2
- 235000019341 magnesium sulphate Nutrition 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000004014 plasticizer Substances 0.000 description 2
- 239000001103 potassium chloride Substances 0.000 description 2
- 235000011164 potassium chloride Nutrition 0.000 description 2
- 239000013535 sea water Substances 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 150000004945 aromatic hydrocarbons Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000011066 ex-situ storage Methods 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000003305 oil spill Substances 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 230000003716 rejuvenation Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/343—Biological treatment of water, waste water, or sewage characterised by the microorganisms used for digestion of grease, fat, oil
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/344—Biological treatment of water, waste water, or sewage characterised by the microorganisms used for digestion of mineral oil
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- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
- C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
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- C02F2101/30—Organic compounds
- C02F2101/32—Hydrocarbons, e.g. oil
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Abstract
The invention discloses a composite microbial inoculant for treating offshore oil sewage, which comprises the following components: at least two of Bacillus subtilis ACCC10619, bacillus subtilis ACCC10719, lactobacillus plantarum (Lactobacillus plantarum) ACCC03291 and Lactobacillus plantarum (Lactobacillus plantarum) ACCC 11118. The composite bacterial agent provided by the application is compounded by adopting the bacterial strain with good salt tolerance and no antagonism, and the obtained composite bacterial strain has a remarkable degradation effect on petroleum hydrocarbon, and is particularly suitable for marine petroleum sewage with high salt content and mineralization degree; and the composite microbial inoculum is combined with a solid carrier with specific components and proportions, so that the composite microbial inoculum can be quickly and effectively compatible with petroleum sewage, and the treatment effect of the composite microbial inoculum is improved.
Description
Technical Field
The invention relates to the technical field of sewage treatment, in particular to a composite microbial inoculum for treating offshore oil sewage and a preparation method thereof.
Background
The petroleum pollution mostly occurs in the marine environment, and the damage to the ocean and the surrounding ecological environment is extremely serious, so that not only is the large-area sea area anoxic caused, but also the death of fish, shrimp, shellfish and seabirds is caused, the floating oil pollutes the beach, the beach is wasted, the marine culture and the salt field production are damaged, the coastal travel area is destroyed, and the service function and the value of the marine ecological system are reduced.
The petroleum pollution is mainly caused by oil spill accidents at sea and discharge of ship dirty oil, and the marine petroleum sewage has complex pollutant components and contains a large amount of salt, so that great difficulty is added to the treatment of the marine petroleum sewage. The current treatment method of the marine petroleum sewage mainly comprises in-situ treatment and ex-situ treatment, wherein the microbial method is adopted to assist in treating the marine sewage, and the marine petroleum sewage can be metabolized into bacterial components and pollution-free carbon dioxide, so that the method is a more environment-friendly and efficient treatment method.
However, although some microbial bacteria capable of degrading petroleum hydrocarbons have been found in the prior art, the degradation ability of the bacteria is limited in view of the high salt environment formed by seawater, and the oil phase and the water phase in petroleum wastewater have complex compositions, and the conventional treatment agent is difficult to be put into and compatible with petroleum wastewater, which is disadvantageous in improving the treatment efficiency thereof.
Disclosure of Invention
In order to solve the problems, the application aims to provide the composite microbial inoculum which can be suitable for a seawater environment with high salt content, has good compatibility with petroleum sewage and can quickly and effectively degrade petroleum hydrocarbon.
In one aspect, the present application provides a composite microbial inoculant for treating marine petroleum wastewater, the composite microbial inoculant comprising: at least two of bacillus subtilis (Bacillus subtilis) ACCC10619, bacillus subtilis (Bacillus subtilis) ACCC10719, lactobacillus plantarum (Lactobacillus plantarum) ACCC03291, and lactobacillus plantarum (Lactobacillus plantarum) ACCC 11118.
Further, the composite microbial inoculum comprises bacillus subtilis (Bacillus subtilis) ACCC10619 and lactobacillus plantarum (Lactobacillus plantarum) ACCC03291 with a viable bacteria number ratio of 5:1.
Further, the composite microbial inoculum is a solid microbial inoculum, the solid microbial inoculum is prepared by loading bacterial strains on a solid carrier, and the total number of loaded living bacteria is not less than 1 multiplied by 10 8 CFU/g。
Further, the solid carrier comprises, by weight, 10-20 parts of activated sludge, 3-4 parts of dodecyl alcohol polyoxyethylene (9) ether, 1-2 parts of sodium dodecyl sulfonate, 5-10 parts of microcrystalline cellulose and 8-12 parts of polyacrylate.
The composite strain provided by the application is obtained by taking degradation of diesel oil as an index on the basis of knowing that each strain has certain salt tolerance and performing experimental screening; the activated sludge in the solid carrier can provide an adhesion and culture carrier for the bacterial strain, and the emulsifier dodecyl alcohol polyoxyethylene (9) ether and sodium dodecyl sulfonate can further wrap an oil phase in petroleum sewage, so that the composite bacterial strain can play a better role; microcrystalline cellulose is used as a filler, has a good oil adsorption effect, and assists the emulsifier to adsorb and wrap the oil phase; the polyacrylate is used as a plasticizer, so that loose sedimentation of the carrier after being put in can be effectively prevented, and the loss of the strain on the degradation effect of the oil phase is avoided.
On the other hand, the application also provides a preparation method of the composite microbial inoculum for treating the offshore oil sewage, which comprises the following steps: uniformly mixing bacterial liquid containing the bacterial strain with activated sludge, sequentially adding microcrystalline cellulose, dodecyl alcohol polyoxyethylene (9) ether, sodium dodecyl sulfonate and polyacrylate, uniformly mixing to obtain a mixed material, and extruding and granulating the mixed material to obtain the composite bacterial agent loaded with the bacterial strain.
On the other hand, the application also provides a method for treating the marine petroleum sewage by using the composite microbial inoculum, which comprises the step of adding the composite microbial inoculum into the marine petroleum sewage, wherein the salt content of the marine petroleum sewage is not less than 30%, the mineralization degree is not less than 10%, the oil content is not less than 60ppm, and the COD value is 300-350 ppm.
The beneficial effects of this application lie in:
the composite bacterial agent provided by the application is compounded by adopting the bacterial strain with good salt tolerance and no antagonism, and the obtained composite bacterial strain has a remarkable degradation effect on petroleum hydrocarbon, and is particularly suitable for marine petroleum sewage with high salt content and mineralization degree; and the composite microbial inoculum is combined with a solid carrier with specific components and proportions, so that the composite microbial inoculum can be quickly and effectively compatible with petroleum sewage, and the treatment effect of the composite microbial inoculum is improved.
Detailed Description
In the following description, numerous specific details are set forth in order to provide a more thorough understanding of the present invention. It will be apparent, however, to one skilled in the art that the invention may be practiced without one or more of these details. In other instances, well-known features have not been described in detail in order to avoid obscuring the invention.
Unless otherwise indicated, the experimental set-up referred to in the examples below were conventional equipment commercially available and the methods of the experiments were all known in the art.
In an embodiment of the present application, bacillus subtilis (Bacillus subtilis) and lactobacillus plantarum (Lactobacillus plantarum), which are known to have a certain salt tolerance, are selected and compounded to obtain a strain without antagonism, and specific strain type selection and screening methods are described in example 1 of patent CN109400222 a.
According to antagonism experiments among strains, the bacillus subtilis (Bacillus subtilis) strains ACCC10619 and ACCC10719 and the lactobacillus plantarum (Lactobacillus plantarum) strains ACCC03291 and ACCC11118 have no antagonism, and can be compounded pairwise. Wherein, the four strains are all purchased from China center for type culture Collection of microorganisms.
Example 1 screening of composite strains
The commercially available bacillus subtilis (Bacillus subtilis) strains ACCC10619, ACCC10719, lactobacillus plantarum (Lactobacillus plantarum) strains ACCC03291 and ACCC11118 were subjected to activation culture: inoculating glycerol tube strains of each strain stored at-80 ℃ into 5mL of HLB liquid culture medium, placing the glycerol tube strains into a shaking table filled with 30 ℃ and 200r/min for culturing for 72 hours, then respectively streaking activated bacterial liquid into HLB solid culture medium, placing the solid culture medium into a constant temperature incubator at 30 ℃ for rejuvenating and culturing for 24 hours, and respectively inoculating bacterial colonies on a flat plate into the shaking table filled with 5mL of HLB liquid culture medium and culturing for 12 hours at 30 ℃ and 200r/min to obtain seed liquid of each strain.
Respectively separating the seed solution of the four activated strainsThe content of viable bacteria is 1 multiplied by 10 prepared by deionized water 8 The CFU/mL bacterial solutions were designated as A1 strain (Bacillus subtilis (Bacillus subtilis) strain ACCC 10619), A2 strain (Bacillus subtilis (Bacillus subtilis) strain ACCC 10719), B1 strain (Lactobacillus plantarum (Lactobacillus plantarum) strain ACCC 03291) and B2 strain (Lactobacillus plantarum (Lactobacillus plantarum) strain ACCC 11118), respectively.
Mixing the bacterial liquid with activated sludge (supplied by Su's Ind. Bo's environmental protection technology Co., ltd.) to obtain a final activated sludge with a viable count of 1×10 8 CFU/g, obtaining bacterial agent, and respectively putting bacterial agent into three beakers with the following solutions, wherein the input amount is 10g, namely, the total input amount of added living bacteria is 1×10 9 CFU：
1# beaker: 200g of 0# diesel;
2# beaker: 200g of 0# diesel oil, 20g of sodium chloride, 10g of magnesium sulfate, 1g of potassium chloride and 1g of calcium chloride;
3# beaker: 200g of 0# diesel oil, 20g of sodium chloride, 10g of magnesium sulfate, 1g of potassium chloride, 1g of calcium chloride and 500g of deionized water.
After the bacterial preparation was put into the beaker, the beaker was left to stand at normal temperature for 3 days, and then the residual oil amount in the beaker (including straight-chain hydrocarbons of C11-C24 (without C23), branched-chain hydrocarbons of C15 and C19, aromatic hydrocarbons of C9-C12) was detected, and the degradation rate was calculated, and the result was retained to the position after the decimal point, and the obtained result is shown in Table 1.
TABLE 1
| Example | Bacterial liquid and viable bacteria quantity ratio | 1# beaker | 2# beaker | 3# beaker |
| 1 | A1 | 75.1％ | 73.4％ | 48.9％ |
| 2 | A2 | 18.6％ | 10.2％ | 2.4％ |
| 3 | B1 | 65.3％ | 40.5％ | 26.8％ |
| 4 | B2 | 67.4％ | 59.2％ | 31.7％ |
| 5 | A1：B1＝1:1 | 88.5％ | 85.9％ | 67.3％ |
| 6 | A1：B2＝1:1 | 76.8％ | 62.4％ | 45.7％ |
| 7 | B1：B2＝1:1 | 66.7％ | 55.3％ | 28.4％ |
| 8 | A1：B1＝1:2 | 81.4％ | 72.6％ | 58.6％ |
| 9 | A1：B1＝2:1 | 90.5％ | 89.6％ | 70.9％ |
| 10 | A1：B1＝5:1 | 98.2％ | 97.4％ | 75.5％ |
| 11 | A1：B1＝7:1 | 82.8％ | 78.5％ | 63.2％ |
| D1 | Sterile liquid and blank activated sludge | 12.3％ | 8.6％ | 1.7％ |
As can be seen from the data in table 1, the added strains and the ratio of the number of viable bacteria thereof are different, and the difference between the degradation capacities of the strains on diesel oil is large, wherein when the A1 bacterial liquid (bacillus subtilis (Bacillus subtilis) strain ACCC 10619) and the B1 bacterial liquid (lactobacillus plantarum (Lactobacillus plantarum) strain ACCC 11118) with the ratio of the number of viable bacteria of 5:1 are selected, the degradation rate of the strains on diesel oil can be as high as 98.2%, and the degradation effect is hardly affected under the condition of high salt content and mineralization.
However, in the 3# beaker containing both diesel and water, the degradation effect of each microbial inoculum was significantly affected, presumably due to the degradation efficiency reduction caused by the easy sedimentation of the activated sludge in the oil-water mixed phase, and thus the solid carrier of the microbial inoculum was further optimized in the following examples.
Example 2 preparation of Complex microbial preparation
The composite bacterial preparation provided by the embodiment is a solid bacterial preparation, and the preparation method of the solid bacterial preparation is as follows:
according to the mass portion, the composite bacterial liquid of A1 bacterial liquid and B1 bacterial liquid with the living bacterial quantity ratio of 5:1 is uniformly mixed with 15 portions of activated sludge, then 8 portions of filler, 5 portions of emulsifying agent and 10 portions of plasticizer are sequentially added, after uniform mixing, the mixed material is extruded to prepare particles with the particle size of about 2mm, and the total living bacterial quantity of the loaded bacterial strain is about 1 multiplied by 10 10 CFU/g。
50g of composite bacterial preparation particles are taken and put into 1kg of marine petroleum sewage, the salt content of the marine petroleum sewage is about 35%, the mineralization degree is about 12%, the oil content is about 100ppm, and the COD value is 400ppm. After the addition is completed, standing for 3 days at normal temperature, and then detecting the residual oil quantity and COD value in the sewage, wherein the result is kept to an integer position.
TABLE 2
As is clear from the results shown in Table 2, the composite bacterial preparation particles produced by using different solid carriers have different dispersibility and compatibility in offshore oil wastewater. The composite bacterial preparation particles prepared in example 16 have the best treatment effect on high-salt and high-mineralization offshore petroleum sewage, and after the composite bacterial preparation is used for 3 days, the oil content in the sewage is measured to be less than 1ppm, the degradation rate is up to 99%, the COD value is reduced to be less than 10ppm, and the national emission standard is met.
The foregoing is merely exemplary of the present application and is not intended to limit the present application. Various modifications and changes may be made to the present application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc. which are within the spirit and principles of the present application are intended to be included within the scope of the claims of the present application.
Claims (3)
1. A composite microbial agent for treating marine petroleum sewage is characterized by comprising bacillus subtilis with a viable bacteria number ratio of 5:1Bacillus subtilis) ACCC10619 and Lactobacillus plantarumLactobacillus plantarum）ACCC03291；
The composite bacterial agent is a solid bacterial agent, the solid bacterial agent is prepared by loading bacterial strains on a solid carrier, and the total number of loaded living bacteria is not less than 1 multiplied by 10 8 CFU/g；
The solid carrier comprises, by weight, 10-20 parts of activated sludge, 3-4 parts of dodecyl alcohol polyoxyethylene (9) ether, 1-2 parts of sodium dodecyl sulfonate, 5-10 parts of microcrystalline cellulose and 8-12 parts of polyacrylate.
2. The method for preparing the composite microbial inoculum for treating offshore oil sewage according to claim 1, which is characterized by comprising the following steps: uniformly mixing bacterial liquid containing the bacterial strain with activated sludge, sequentially adding microcrystalline cellulose, dodecyl alcohol polyoxyethylene (9) ether, sodium dodecyl sulfonate and polyacrylate, uniformly mixing to obtain a mixed material, and extruding and granulating the mixed material to obtain the composite bacterial agent loaded with the bacterial strain.
3. A method for treating marine petroleum wastewater by using the composite microbial agent according to claim 1, which comprises the step of adding the composite microbial agent to the marine petroleum wastewater, wherein the salt content of the marine petroleum wastewater is not less than 30%, the mineralization degree is not less than 10%, the oil content is not less than 60ppm, and the cod value is 300-350 ppm.
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| CN108203701A (en) * | 2016-12-19 | 2018-06-26 | 中国科学院深圳先进技术研究院 | Complex microbial inoculum and its preparation method and application |
| CN108624538A (en) * | 2018-06-07 | 2018-10-09 | 广州中国科学院先进技术研究所 | A kind of composite bacteria agent and preparation method thereof for efficient process offshore oil sewage |
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| CN108203701A (en) * | 2016-12-19 | 2018-06-26 | 中国科学院深圳先进技术研究院 | Complex microbial inoculum and its preparation method and application |
| CN108624538A (en) * | 2018-06-07 | 2018-10-09 | 广州中国科学院先进技术研究所 | A kind of composite bacteria agent and preparation method thereof for efficient process offshore oil sewage |
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