CN114029098B

CN114029098B - Movable PCR laboratory

Info

Publication number: CN114029098B
Application number: CN202111308098.3A
Authority: CN
Inventors: 张国昀; 何彩云; 张建国; 段爱国
Original assignee: Research Institute of Forestry of Chinese Academy of Forestry
Current assignee: Research Institute of Forestry of Chinese Academy of Forestry
Priority date: 2021-11-05
Filing date: 2021-11-05
Publication date: 2022-10-11
Anticipated expiration: 2041-11-05
Also published as: CN114029098A

Abstract

The invention discloses a movable PCR laboratory, which comprises a preparation mechanism, an operating mechanism and an adjusting mechanism; the operating mechanism is connected to one side surface of the preparation mechanism in a sliding mode, the operating mechanism can be operated inside the preparation mechanism, the adjusting mechanism is located on the inner top wall of the preparation mechanism, and the adjusting mechanism can adjust the space inside the preparation mechanism; the invention is divided into four areas for experiment, the four areas are mutually independent under the condition of different ventilation, the air in an analysis area can be prevented from flowing back to the front end area during ventilation, the pollution of DNA aerosol to the front end area is effectively prevented, and simultaneously, under the action of the negative pressure forming plate and the sealing rubber, the air in the sucker is pumped away, so that the negative pressure is formed in the sucker, the sealing leakage between the box body and the experiment table can be prevented.

Description

Movable PCR laboratory

Technical Field

The invention relates to the technical field of medical instruments. In particular a mobile PCR laboratory.

Background

Present PCR laboratory is when using, the PCR product aerosol of high concentration can appear, these aerosols are brought into the prozone of amplification by the zone after the amplification easily through blowing or operating personnel maloperation, and then cause the pollution to the reagent, present device leads to the direction of air current to carry out autonomic control because reasons such as sealed, and each production zone of PCR laboratory is separately set up, work efficiency has been reduced, present device can only be used in specific place, lead to the use of device to appear the limitation, consequently still have very big shortcoming.

Disclosure of Invention

Therefore, the present invention is to provide a mobile PCR laboratory that can prevent aerosol contamination and is suitable for use in various planes.

In order to solve the technical problems, the invention provides the following technical scheme:

the mobile PCR laboratory comprises a preparation mechanism, an operation mechanism and an adjusting mechanism; the operating mechanism is connected to one side face of the preparation mechanism in a sliding mode, the operating mechanism can be operated inside the preparation mechanism, the adjusting mechanism is located on the inner top wall of the preparation mechanism, and the adjusting mechanism can adjust the space inside the preparation mechanism.

In the mobile PCR laboratory, the preparation mechanism comprises a box body, a preparation area, a sample processing area, an amplification area, an analysis area, a protective shell, a fan, an air inlet, a suction cup, a baffle, a vent hole, a limiting plate, a sealing layer, a rotating plate, a fixing plate, a sealing spring, sealing rubber, a negative pressure forming plate, a compression spring, a sliding tube, a cavity, a suction cup and a positioning ring; the preparation area, the sample processing area, the amplification area and the analysis area are all positioned in the box body, the inlet of the sample processing area is hermetically connected and communicated with the outlet of the preparation area, the inlet of the amplification area is hermetically connected and communicated with the outlet of the sample processing area, and the inlet of the analysis area is hermetically connected and communicated with the outlet of the amplification area; one side of the protective shell is hermetically connected and communicated with an inlet of the preparation area, the fan is positioned in the protective shell, and the air inlet hole is positioned on one side surface of the preparation area; the baffle and the fixed plate are positioned in the box body, a gap is reserved between the baffle and the fixed plate, the quantity of the baffle and the fixed plate is two or two, one baffle and one fixed plate form a group, the baffle and the fixed plate in each group divide the interior of the box body into the preparation area, the sample processing area, the amplification area and the analysis area, the vent hole is positioned at one end of the baffle, the limiting plate is positioned on one side face of the baffle opposite to the fixed plate, the rotating plate is rotatably connected in the fixed plate, the sealing layer is fixedly installed on the outer wall of the rotating plate, one end of the rotating plate can be in contact with the limiting plate, the end of the rotating plate in contact with the limiting plate is arc-shaped, the outer wall of the other end of the rotating plate is fixedly connected with one end of the sealing spring, and the other end of the sealing spring is fixedly connected with the inner wall of the fixed plate; the sealing rubber is positioned at the inner bottom of the baffle, a sliding groove is formed in the sealing rubber, the negative pressure forming plate is connected with the inside of the sliding groove in the sealing rubber in a sliding mode, the compression spring is sleeved on the outer wall of the sliding pipe, one end of the compression spring is fixedly connected with one end of the negative pressure forming plate, the other end of the compression spring is fixedly connected with one end face of the positioning ring, one end face of the positioning ring is connected with one end, positioned in the baffle, of the sealing rubber, one end of the sliding pipe is fixedly connected with one end of the negative pressure forming plate, the other end of the sliding pipe penetrates through the inside of the positioning ring and is positioned in the cavity, the cavity is positioned in the baffle, the sucker is positioned at the bottom of the baffle, and the other end of the negative pressure forming plate is positioned in the sucker; the preparation mechanism is divided into four areas for experiment, and the preparation area blows to the sample processing area, the amplification area and the analysis area in sequence, make preparation area and sample processing area be in the wind gap all the time, make mutual independence under the condition of different ventilation in four areas through setting up of rotor plate, can prevent that the wind of analysis area from pouring into the region of front end when ventilating simultaneously, effectively prevent the pollution of DNA aerosol to the front end region, simultaneously under the effect of negative pressure formation board and sealing rubber, make the air in the sucking disc be taken away, make the sucking disc form the negative pressure, can prevent to appear sealedly revealing between box and the laboratory bench, and then the condition of device experimental data is influenced, this device is applicable to sealed the fixing on different laboratory benches and different concave-convex planes simultaneously, the optional application place is many, and under the effect of sucking disc, make this device be convenient for moving, high durability and convenient use, and this device can work simultaneously, but mutual noninterference, make the device experiment more convenient.

In the mobile PCR laboratory, the operating mechanism includes a slide box, a sealing cylinder, a sealing ring, an operating glove, a slide block, a screw rod, a first ultraviolet lamp, a washing tube, a rotary cylinder, a folding plate and an arc plate; the slide box is positioned on one end face of the box body, the sealing barrel is connected inside the slide box in a sliding mode, the sealing ring is positioned on one end face of the slide box and is connected with one end of the sealing barrel in a sealing mode, the other end of the sealing barrel is connected with the operating gloves in a sealing mode, one end of the sliding block is fixedly connected with the outer wall of the other end of the sealing barrel, the sliding block is connected on the outer wall of the lead screw in a sliding mode, the lead screw is connected inside the slide box in a rotating mode and is positioned at two ends of the sealing barrel, the first ultraviolet lamp is positioned at the inner top of the slide box, the flushing pipe is also positioned at the inner top of the slide box and is positioned at two ends of the first ultraviolet lamp, two ends of the slide box are respectively fixedly connected with one end of the folding plate, the other end of the folding plate is connected with the arc plate in a sealing mode, and the arc plate is connected inside the rotating barrel in a sliding mode; through the setting of slip case, can be convenient for carry out the displacement at the surface of box, and then make operating gloves can operate the region of difference, make this device only need an operating gloves can accomplish the experiment in all regions, simultaneously at the effect of folded sheet and rotary drum, can prevent to appear sealed phenomenon of revealing between slip case and the box, and the slip case of being convenient for carries out the displacement at the surface of box, through the setting of first purple light lamp and flushing pipe, can wash operating gloves, prevent to cause the pollution between the different regions.

In the mobile PCR laboratory, the adjusting mechanism comprises a gear box, a gear, a transmission belt, a transmission shaft, a sealing ring, a toothed plate, a fixed box, a sealing plate, a collecting box, an adsorption plate, a collecting pipe, a filtering solution, a dispersion pipe, a second ultraviolet lamp and an air outlet; the gearbox is positioned at the top of the box body, the number of the gearboxes is two or more, the gears are rotationally connected with two ends inside the gearbox, two gears are arranged inside one gearbox and are driven by the driving belt, one end of the driving shaft is positioned inside the driving belt, the other end of the driving shaft penetrates through the gearbox and is in sealing connection with the sealing ring, and the sealing ring is positioned on one end face of the gearbox; the toothed plate is meshed with the gear, the toothed plate is positioned in the box body, the fixed box is positioned in the middle of the toothed plate, one ends of the baffle and the fixed plate are fixedly connected with the fixed box, the sealing plates are positioned at two ends of the toothed plate, and the outer surfaces of the sealing plates are hermetically connected with the inner top wall of the box body; the air outlet hole is positioned on the inner wall of one side face of the analysis area and is connected and communicated with the inlet end of the collecting box, the adsorption plate is fixedly installed inside the collecting box, one side face of the adsorption plate is in contact with the inlet of the collecting box, the inlet of the collecting pipe is in contact with the other side face of the adsorption plate, the outlet of the collecting pipe penetrates through the filtering solution and is positioned on the inner bottom wall of the collecting box, the filtering solution is positioned inside the collecting box, the dispersion pipe is positioned on the liquid level of the filtering solution, and the second ultraviolet lamp is positioned on the inner top of the collecting box; through the setting of gear box, can make the gear can drive the pinion rack and carry out the displacement, and then drive baffle and fixed plate and carry out the displacement, thereby change each regional size, when the user area is great, can adjust through the transmission shaft, the conversion of the device of being convenient for, improve the practicality of device, increase its flexibility ability, make the usage space efficiency of device obtain the maximize, simultaneously through the setting of collecting box, can make the tail gas that the device ventilates absorb, then handle, prevent that gaseous direct emission from to the atmosphere in, prevent that the aerosol from causing the pollution to the atmosphere.

The technical scheme of the invention achieves the following beneficial technical effects:

1. the device is divided into four areas for experiment, and the preparation area, the amplification area and the analysis area are blown in sequence from the preparation area to the sample processing area, so that the preparation area and the sample processing area are always positioned at an air inlet, the four areas are mutually independent under the condition of different ventilation through the arrangement of the rotating plate, and the air in the analysis area can be prevented from flowing back to the area at the front end during ventilation, thereby effectively preventing the pollution of DNA aerosol on the front end area.

2. According to the invention, through the arrangement of the sliding box, the displacement can be conveniently carried out on the outer surface of the box body, so that the operation gloves can operate different areas, the experiment in all areas can be completed by only one operation glove, meanwhile, the phenomenon of sealing leakage between the sliding box and the box body can be prevented under the action of the folding plate and the rotary drum, the displacement of the sliding box on the outer surface of the box body is convenient, the operation gloves can be washed through the arrangement of the first ultraviolet lamp and the washing pipe, and the pollution caused between different areas is prevented.

3. According to the invention, through the arrangement of the gear box, the gear can drive the toothed plate to displace, so that the baffle and the fixed plate are driven to displace, the size of each region is changed, when the use region is larger, the gear can be adjusted through the transmission shaft, the conversion of the device is facilitated, the practicability of the device is improved, the flexibility of the device is improved, the use space efficiency of the device is maximized, and meanwhile, through the arrangement of the collecting box, the tail gas ventilated by the device can be absorbed and then treated, the gas is prevented from being directly discharged into the atmosphere, and the pollution of aerosol to the atmosphere is prevented.

Drawings

FIG. 1 is a schematic view of the interior of the present invention in elevation;

FIG. 2 is an enlarged schematic view of FIG. 1 at A according to the present invention;

FIG. 3 is a schematic structural view of the inside of the slide case of the present invention;

FIG. 4 is a front view of the operating mechanism of the present invention;

FIG. 5 is a schematic view of the construction of the folding plate and drum of the present invention;

FIG. 6 is an enlarged schematic view of the invention at B of FIG. 5;

FIG. 7 is a schematic top view of the drum of the present invention.

The reference numbers in the figures denote: 100-a preparation mechanism; 200-an operating mechanism; 300-an adjustment mechanism; 101-a box body; 102-a preparation area; 103-a sample treatment zone; 104-an amplification region; 105-an analysis zone; 106-protective shell; 107-a fan; 108-air inlet holes; 109-a sucker; 110-a baffle; 111-a vent; 112-a limiting plate; 113-a sealing layer; 114-a rotating plate; 115-a fixation plate; 116-a sealing spring; 117-sealing rubber; 118-negative pressure forming plate; 119-a compression spring; 120-a slide tube; 121-a cavity; 122-a suction cup; 123-positioning rings; 201-a slide box; 202-sealing cylinder; 203-sealing ring; 204-handling gloves; 205-a slider; 206-a lead screw; 207-first uv lamp; 208-a flush tube; 209-drum; 210-folding a plate; 211-arc plate; 301-a gearbox; 302-a gear; 303-a transmission belt; 304-a drive shaft; 305-a sealing ring; 306-toothed plate; 307-stationary box; 308-a sealing plate; 309-collecting box; 310-an adsorption plate; 311-a collection tube; 312-filtering the solution; 313-a dispersion tube; 314-a second uv lamp; 315-air outlet.

Detailed Description

The mobile PCR laboratory comprises a preparation mechanism 100, an operating mechanism 200 and an adjusting mechanism 300; the operation mechanism 200 is slidably connected to a side surface of the preparation mechanism 100, the operation mechanism 200 is operable inside the preparation mechanism 100, the adjustment mechanism 300 is located on an inner top wall of the preparation mechanism 100, and the adjustment mechanism 300 can adjust a space inside the preparation mechanism 100.

As shown in fig. 1-2, the preparation mechanism 100 includes a case 101, a preparation area 102, a sample processing area 103, an amplification area 104, an analysis area 105, a protective case 106, a blower 107, an air inlet 108, a suction cup 109, a baffle 110, a vent hole 111, a stopper plate 112, a sealing layer 113, a rotating plate 114, a fixing plate 115, a sealing spring 116, a sealing rubber 117, a negative pressure forming plate 118, a compression spring 119, a slide tube 120, a cavity 121, a suction cup 122, and a retainer ring 123; the preparation area 102, the sample processing area 103, the amplification area 104 and the analysis area 105 are all positioned inside the box body 101, the inlet of the sample processing area 103 is hermetically connected and communicated with the outlet of the preparation area 102, the inlet of the amplification area 104 is hermetically connected and communicated with the outlet of the sample processing area 103, and the inlet of the analysis area 105 is hermetically connected and communicated with the outlet of the amplification area 104; one side of the protective shell 106 is hermetically connected and communicated with an inlet of the preparation area 102, the fan 107 is located inside the protective shell 106, and the air inlet 108 is located on one side surface of the preparation area 102; the baffle 110 and the fixed plate 114 are both positioned inside the box body 101, a gap is reserved between the baffle 110 and the fixed plate 114, the number of the baffle 110 and the fixed plate 114 is two or more, one baffle 110 and one fixed plate 115 form a group, each group of baffle 110 and fixed plate 115 divides the interior of the box body 101 into a preparation area 102, a sample processing area 103, an amplification area 104 and an analysis area 105, the vent hole 111 is positioned at one end of the baffle 110, the limiting plate 112 is positioned at one side face of the baffle 110 opposite to the fixed plate 115, the rotating plate 114 is rotatably connected inside the fixed plate 115, the sealing layer 113 is fixedly arranged on the outer wall of the rotating plate 114, one end of the rotating plate 114 can be contacted with the limiting plate 112, the end of the rotating plate 114 contacted with the limiting plate 112 is arc-shaped, the outer wall of the other end of the rotating plate 114 is fixedly connected with one end of the sealing spring 116, and the other end of the sealing spring 116 is fixedly connected with the inner wall of the fixed plate 115; the sealing rubber 117 is positioned at the inner bottom of the baffle 110, a sliding groove is formed in the sealing rubber 117, the negative pressure forming plate 118 is connected in the sliding groove in the sealing rubber 117 in a sliding mode, the compression spring 119 is sleeved on the outer wall of the sliding pipe 120, one end of the compression spring 119 is fixedly connected with one end of the negative pressure forming plate 118, the other end of the compression spring 119 is fixedly connected with one end face of the positioning ring 123, one end face of the positioning ring 123 is connected with one end, positioned in the baffle 110, of the sealing rubber 117, one end of the sliding pipe 120 is fixedly connected with one end of the negative pressure forming plate 118, the other end of the sliding pipe 120 penetrates through the positioning ring 123 and is positioned in the cavity 121, the cavity 121 is positioned in the baffle 110, the sucker 122 is positioned at the bottom of the baffle 110, and the other end of the negative pressure forming plate 118 is positioned in the sucker 122; the preparation mechanism 100 is divided into four areas for experiment, and the preparation area 102 blows air to the sample processing area 103, the amplification area 104 and the analysis area 105 in sequence, so that the preparation area 102 and the sample processing area 103 are always positioned at an air inlet, the four areas are mutually independent under different ventilation conditions through the arrangement of the rotating plate 114, the air of the analysis area 105 can be prevented from flowing backwards to the front end area during ventilation, the pollution of DNA aerosol to the front end area can be effectively prevented, meanwhile, under the action of the negative pressure forming plate 118 and the sealing rubber 117, the air in the sucker 122 is pumped away, negative pressure is formed in the sucker 122, the sealing leakage between the box body 101 and the experiment table can be prevented, and the condition of experimental data of the device is influenced.

As shown in fig. 3, the operating mechanism 200 includes a slide box 201, a sealing cylinder 202, a sealing ring 203, an operating glove 204, a slider 205, a lead screw 206, a first ultraviolet lamp 207, a washing tube 208, a rotating cylinder 209, a folding plate 210, and an arc plate 211; the slide box 201 is located on one end face of the box body 101, the sealing cylinder 202 is connected to the inside of the slide box 201 in a sliding mode, the sealing ring 203 is located on one end face of the slide box 201, the sealing ring 203 is connected with one end of the sealing cylinder 202 in a sealing mode, the other end of the sealing cylinder 202 is connected with the operating glove 204 in a sealing mode, one end of the sliding block 205 is fixedly connected with the outer wall of the other end of the sealing cylinder 202, the sliding block 205 is connected to the outer wall of the lead screw 206 in a sliding mode, the lead screw 206 is connected to the inside of the slide box 201 in a rotating mode and located at two ends of the sealing cylinder 202 in a rotating mode, the first ultraviolet lamp 207 is located at the inner top of the slide box 201, the flushing pipe 208 is also located at the inner top of the slide box 201 and located at two ends of the first ultraviolet lamp 207, as shown in fig. 4-7, two ends of the slide box 201 are respectively fixedly connected with one end of the folding plate 210, the other end of the folding plate 210 is connected with the arc plate 211 in a sealing mode, and the arc plate 211 is connected to the inside of the rotating cylinder 209 in a sliding mode; through the setting of slip box 201, can be convenient for carry out the displacement at the surface of box 101, and then make operation gloves 204 can operate the region of difference, make this device only need an operation gloves 204 can accomplish the experiment in all regions, simultaneously at folding plate 210 and rotary drum 209's effect, can prevent to appear sealed phenomenon of revealing between slip box 201 and the box 101, and be convenient for slip box 201 carries out the displacement at the surface of box 101, through the setting of first purple light lamp 207 and flushing pipe 208, can wash operation gloves 204, prevent to cause the pollution between the different regions.

As shown in fig. 1, the adjusting mechanism 300 includes a gear box 301, a gear 302, a transmission belt 303, a transmission shaft 304, a sealing ring 305, a toothed plate 306, a fixed box 307, a sealing plate 308, a collection box 309, an adsorption plate 310, a collection pipe 311, a filtered solution 312, a dispersion pipe 313, a second ultraviolet lamp 314, and an air outlet 315; the gear boxes 301 are positioned at the top of the box body 101, the number of the gear boxes 301 is two or more, the gears 302 are rotatably connected to two ends inside the gear boxes 302, two gears 302 are arranged inside one gear box 301, the two gears 302 are driven by a driving belt 303, one end of a driving shaft 304 is positioned inside the driving belt 303, the other end of the driving shaft 304 penetrates through the gear boxes 301 and is in sealing connection with a sealing ring 305, and the sealing ring 305 is positioned on one end face of the gear box 302; the toothed plate 306 is in meshed connection with the gear 302, the toothed plate 306 is located inside the box body 101, the fixed box 307 is located in the middle of the toothed plate 306, one ends of the baffle plate 110 and the fixed plate 115 are both fixedly connected with the fixed box 307, the sealing plates 308 are located at two ends of the toothed plate 306, and the outer surfaces of the sealing plates 308 are in sealed connection with the inner top wall of the box body 101; the air outlet 315 is located on the inner wall of one side of the analysis area 105 and is connected and communicated with the inlet end of the collection box 309, the adsorption plate 310 is fixedly installed inside the collection box 309, one side of the adsorption plate 310 is in contact with the inlet of the collection box 309, the inlet of the collection pipe 311 is in contact with the other side of the adsorption plate 310, the outlet of the collection pipe 311 penetrates through the filtering solution 312 and is located on the inner bottom wall of the collection box 309, the filtering solution 312 is located inside the collection box 309, the dispersion pipe 313 is located on the liquid level of the filtering solution 312, and the second ultraviolet lamp 314 is located on the inner top of the collection box 309; through gear box 301's setting, can make gear 302 can drive pinion rack 306 and carry out the displacement, and then drive baffle 110 and fixed plate 115 and carry out the displacement, thereby change each regional size, when the user area is great, can adjust through transmission shaft 304, the conversion of the device of being convenient for, improve the practicality of device, increase its flexibility ability, make the usage space efficiency of device obtain the maximize, simultaneously through the setting of collecting box 309, can make the tail gas that the device ventilates absorb, then handle, prevent that gaseous direct emission from to the atmosphere, prevent that aerosol from causing the pollution to the atmosphere.

The working principle is as follows: when the device is used, in order to prevent the phenomenon that aerosol pollution is caused by the cross connection of air flows, firstly, the fan 107 is started, so that external air is blown to the sample processing area 103, the amplification area 104 and the analysis area 105 from the preparation area 102 in sequence, the preparation area 102 and the sample processing area 103 are always positioned at an air inlet, in order to prevent the phenomenon that air flows backwards, the rotating plate 114 is pushed to rotate when the air flows, the sealing spring 116 is stressed and contracted at the moment, an air flow channel is formed between the baffle plate 110 and the fixing plate 115, when the phenomenon that air flows backwards occurs, the rotating plate 114 rotates upwards, the sealing spring 116 loses force at the moment, the sealing layer 113 is hermetically connected with the limiting plate 112, the air flow is prevented from flowing backwards, and meanwhile, when the rotating plate 114 and the limiting plate 112 are sealed, the four areas are mutually independent under the condition of different ventilation, so that the pollution of DNA aerosol to the front end area is effectively prevented, when the device is fixed, the suction cup 112 is contacted with the experiment table for suction, at the moment, air is absorbed through the negative pressure forming plate 118 to pump air in the suction cup 112, so that the suction cup 112 is in sealing fit with the experiment table, the device is suitable for sealing fixation on different experiment tables and different concave-convex planes, multiple application fields can be selected, when in operation, the sliding box 201 is used for operation in different areas, simultaneously, when in area replacement, the lead screw 206 is rotated to enable the sealing cylinder 202 to slide to drive the operating glove 204 to shrink, and simultaneously, the cleaning tube 208 and the first ultraviolet lamp 207 are used for sterilization and flushing, and simultaneously, in the process, because the rotating cylinder 209 is in sealing connection with the folding plate 210, the phenomenon of sealing leakage can be prevented, and meanwhile, the sealing cylinder 202 is in sealing connection with the operating glove 204, therefore, the other end of the sealing cylinder 202 does not need to be sealed, it is convenient to insert the operation glove 204 for operation, in order to maximize the space utilization efficiency of the device, the transmission shaft 304 is rotated to drive the transmission belt 303 to drive the gear 302 to rotate, and then the toothed plate 306 is driven to displace, so that the positions of the baffle 110 and the fixing plate 115 are adjusted, and further the space between different regions is adjusted, so that the space of different regions is maximally utilized, meanwhile, the sealing plate 308 can prevent the toothed plate 306 from being sealed and leaking, the tail gas after operation is collected by the collecting box 309, firstly is adsorbed by the adsorption plate 310, and then is filtered by the filtering solution 312, the tail gas flows out through the dispersion pipe 313 and then is irradiated again through the second ultraviolet lamp 314, aerosol is eliminated, and then the air after cleaning is exhausted to the atmosphere.

It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. This need not be, nor should it be exhaustive of all embodiments. And obvious variations or modifications are possible which remain within the scope of the appended claims.

Claims

1. The mobile PCR laboratory is characterized by comprising a preparation mechanism (100), an operating mechanism (200) and an adjusting mechanism (300);

the operating mechanism (200) is connected to one side surface of the preparation mechanism (100) in a sliding mode, the operating mechanism (200) can be operated inside the preparation mechanism (100), the adjusting mechanism (300) is located on the inner top wall of the preparation mechanism (100), and the adjusting mechanism (300) can adjust the space inside the preparation mechanism (100);

the preparation mechanism (100) comprises a box body (101), a preparation area (102), a sample processing area (103), an amplification area (104), an analysis area (105), a protective shell (106), a fan (107), an air inlet hole (108), a baffle plate (110), a vent hole (111), a limiting plate (112), a sealing layer (113), a rotating plate (114), a fixing plate (115), a sealing spring (116), sealing rubber (117), a negative pressure forming plate (118), a compression spring (119), a sliding pipe (120), a cavity (121), a sucking disc (122) and a positioning ring (123);

the preparation area (102), the sample processing area (103), the amplification area (104) and the analysis area (105) are all positioned inside the box body (101), the inlet of the sample processing area (103) is hermetically connected and communicated with the outlet of the preparation area (102), the inlet of the amplification area (104) is hermetically connected and communicated with the outlet of the sample processing area (103), and the inlet of the analysis area (105) is hermetically connected and communicated with the outlet of the amplification area (104);

one side of the protective shell (106) is hermetically connected and communicated with an inlet of the preparation area (102), the fan (107) is positioned inside the protective shell (106), and the air inlet hole (108) is positioned on one side surface of the preparation area (102);

the baffle (110) and the fixing plate (115) are both positioned inside the box body (101), and a gap is left between the baffle (110) and the fixing plate (115), the number of the baffle plates (110) and the fixing plates (115) is more than two, one baffle plate (110) and one fixing plate (115) form one group, each group of the baffle plates (110) and the fixing plates (115) divides the interior of the box body (101) into the preparation area (102), the sample processing area (103), the amplification area (104) and the analysis area (105), the vent hole (111) is located at one end of the baffle plate (110), the limiting plate (112) is positioned on one side surface of the baffle plate (110) opposite to the fixing plate (115), the rotating plate (114) is rotatably connected to the inner part of the fixed plate (115), the sealing layer (113) is fixedly arranged on the outer wall of the rotating plate (114), one end of the rotating plate (114) can be contacted with the limit plate (112), and one end of the rotating plate (114) contacting with the limiting plate (112) is arc-shaped, the outer wall of the other end of the rotating plate (114) is fixedly connected with one end of the sealing spring (116), the other end of the sealing spring (116) is fixedly connected with the inner wall of the fixing plate (115);

sealing rubber (117) are located the interior bottom of baffle (110), the spout has been seted up to sealing rubber (117) inside, negative pressure forms board (118) sliding connection be in sealing rubber (117) inside the inside of spout, compression spring (119) cup joint on the outer wall of slide tube (120), compression spring (119) one end with the one end fixed connection of negative pressure formation board (118), compression spring (119) the other end with a terminal surface fixed connection of position circle (123), the terminal surface of position circle (123) with sealing rubber (117) are located the inside one end of baffle (110) and are connected, the one end of slide tube (120) with the one end fixed connection of negative pressure formation board (118), the other end of slide tube (120) passes the inside of position circle (123), and is located the inside of cavity (121), cavity (121) are located the inside of baffle (110), sucking disc (122) are located the bottom of baffle (110), the other end of negative pressure formation board (118) is located sucking disc (122) the inside of sucking disc (122).

2. The transportable PCR laboratory according to claim 1, characterized in that the handling mechanism (200) comprises a slide box (201), a sealing cylinder (202), a sealing ring (203), a handling glove (204), a slide block (205), a lead screw (206), a first uv lamp (207), a washing tube (208), a drum (209), a folding plate (210) and an arc plate (211);

the slide box (201) is located on one end face of the box body (101), the sealing cylinder (202) is connected inside the slide box (201) in a sliding mode, the sealing ring (203) is located on one end face of the slide box (201), the sealing ring (203) is connected with one end of the sealing cylinder (202) in a sealing mode, the other end of the sealing cylinder (202) is connected with the operating glove (204) in a sealing mode, one end of the sliding block (205) is fixedly connected with the outer wall of the other end of the sealing cylinder (202), the sliding block (205) is connected on the outer wall of the lead screw (206) in a sliding mode, the lead screw (206) is connected inside the slide box (201) in a rotating mode and located at two ends of the sealing cylinder (202), the first ultraviolet lamp (207) is located at the inner top of the slide box (201), the flushing pipe (208) is also located at the inner top of the slide box (201) and located at two ends of the first ultraviolet lamp (207), the two ends of the slide box (201) are respectively connected with one end of the folding plate (210), and the sliding plate (211) is connected with the inner end of the arc plate (211).

3. The mobile PCR laboratory according to claim 2, wherein the adjusting mechanism (300) comprises a gear box (301), a gear (302), a transmission belt (303), a transmission shaft (304), a sealing ring (305), a toothed plate (306), a fixed box (307), a sealing plate (308), a collection box (309), an adsorption plate (310), a collection pipe (311), a filtration solution (312), a dispersion pipe (313), a second uv lamp (314), and an air outlet (315);

the gear boxes (301) are positioned at the top of the box body (101), the number of the gear boxes (301) is more than two, the gears (302) are rotatably connected to two ends inside the gear boxes (301), two gears (302) are arranged inside one gear box (301), the two gears (302) are driven by the driving belt (303), one end of the driving shaft (304) is positioned inside the driving belt (303), the other end of the driving shaft (304) penetrates through the gear boxes (301) and is in sealing connection with the sealing ring (305), and the sealing ring (305) is positioned on one end face of the gear box (301); the toothed plate (306) is meshed with the gear (302), the toothed plate (306) is positioned inside the box body (101), the fixed box (307) is positioned in the middle of the toothed plate (306), one ends of the baffle plate (110) and the fixed plate (115) are fixedly connected with the fixed box (307), the sealing plates (308) are positioned at two ends of the toothed plate (306), and the outer surfaces of the sealing plates (308) are hermetically connected with the inner top wall of the box body (101);

the exhaust vent (315) is located the inner wall of analysis district (105) a side and with the entry end of collecting box (309) is connected and is led to, adsorption plate (310) fixed mounting be in inside collecting box (309), just a side of adsorption plate (310) with the entry contact of collecting box (309), the entry of collecting pipe (311) with the another side contact of adsorption plate (310), the export of collecting pipe (311) passes filtering solution (312) is located the interior diapire of collecting box (309), filtering solution (312) is located the inside of collecting box (309), dispersion tube (313) are located on the liquid level of filtering solution (312), second purple light lamp (314) are located the interior top of collecting box (309).