CN114028602A - Preparation method of high-elasticity antibacterial nanofiber film for wound healing - Google Patents
Preparation method of high-elasticity antibacterial nanofiber film for wound healing Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/225—Mixtures of macromolecular compounds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/44—Medicaments
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/46—Deodorants or malodour counteractants, e.g. to inhibit the formation of ammonia or bacteria
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F1/00—General methods for the manufacture of artificial filaments or the like
- D01F1/02—Addition of substances to the spinning solution or to the melt
- D01F1/10—Other agents for modifying properties
- D01F1/103—Agents inhibiting growth of microorganisms
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F8/00—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof
- D01F8/04—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from synthetic polymers
- D01F8/14—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from synthetic polymers with at least one polyester as constituent
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/204—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials with nitrogen-containing functional groups, e.g. aminoxides, nitriles, guanidines
- A61L2300/206—Biguanides, e.g. chlorohexidine
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/12—Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces
Abstract
A preparation method of a high-elasticity antibacterial nanofiber film for wound healing comprises the following steps: putting a first mixture and a second mixture which are taken as spinning raw materials into a solvent for dissolving to obtain an electrostatic spinning solution, wherein the first mixture is a mixture of poly (glycerol sebacate) and polycaprolactone which are mixed according to a mass ratio, and the second mixture is a mixture of poly (hexamethylene biguanide hydrochloride) and poly (glycerol sebacate) which are mixed according to a mass ratio; placing the obtained electrostatic spinning solution into an injector of electrostatic spinning equipment by adopting an electrostatic spinning method, applying a high-voltage electrostatic field between an injection needle and a receiving device, enabling the electrostatic spinning solution from the injection needle to pass through the high-voltage electrostatic field and be jetted onto the receiving device in a jet flow form under the state of controlling the flow rate of the electrostatic spinning solution, forming a nanofiber film along with the accumulation on the receiving device, and drying to obtain a finished product. The air permeability effect and the liquid absorbability are good; avoid wound infection and promote wound healing.
Description
Technical Field
The invention belongs to the technical field of preparation of medical wound dressings, and particularly relates to a preparation method of a high-elasticity antibacterial nanofiber film for wound healing.
Background
Wound healing is a way for repairing the injury of tissues and organs, and in the process of repairing the injury of tissues and organs, the medical wound dressing can play a good role in protecting the wound and promoting healing.
The electrostatic spinning method is a method for preparing the polymer solution with charge in a high-voltage electric field by forming accelerated jet flow with the diameter of 10 nm-10 mu m. The non-woven nanofiber membrane for repair prepared by the electrostatic spinning method has the pore diameter of 50 nm-1 mu m generally, so that the non-woven nanofiber membrane has good nanofiber tissue, has very high porosity and void connectivity, and can be used for blocking wound infection caused by bacteria and dust. The sample prepared by electrostatic spinning can simulate the natural extracellular matrix structure to the maximum extent, and can provide ideal conditions for the attachment and proliferation of cells. Many natural materials and artificially synthesized materials can be prepared into a fibrous membrane by an electrostatic spinning method, and as the electrostatic spinning nano-fibers also have the characteristics of excellent specific surface area, porosity and the like, the electrostatic spinning nano-fibers attract the continuous attention of researchers in the field of biomedicine and are well applied to the aspects of wound healing (wound repair), biological tissue engineering and the like.
Polypropanetriol sebacate is a biodegradable biomaterial which is newly synthesized at the beginning of the century and has high elasticity, and is widely used for research in the fields of heart, cartilage and artificial skin due to the characteristic of high elasticity. Compared with polyurethane, the biodegradability of the polyurethane enables the polyurethane to have higher application value in the medical field, but the molecular weight of the polyurethane is small, so that the mechanical strength of the polyurethane is poor, and the polyurethane needs to be compounded with other materials to meet the development requirement of new medical appliance products. Polycaprolactone has excellent mechanical properties and biocompatibility, and is widely used in medical devices. The electrostatic spinning polycaprolactone fiber membrane has high specific surface area and void ratio, can guide the proliferation and differentiation of cells, has high transmittance and ensures the exchange of gas and water. Functional components are added into the fibrous membrane, so that the effects of resisting bacteria and promoting wound healing can be achieved. The polyhexamethylene biguanide hydrochloride is one of biguanide disinfectants and has stronger antibacterial and bactericidal activity. It is of course of good positive significance if the aforementioned different materials can be compounded, in particular by applying the electrospinning technique based on their own characteristics, to produce a wound healing dressing having high elasticity and excellent antibacterial effect, and the technical solutions to be described below have been made in this context.
Disclosure of Invention
The invention aims to provide a preparation method of a high-elasticity antibacterial nanofiber film for wound healing, and the high-elasticity antibacterial nanofiber film for wound healing obtained by the method can embody excellent mechanical strength, elasticity and antibacterial effect, can embody ideal high porosity, air permeability and liquid absorbability, can simulate an extracellular matrix structure by a nano microstructure, directionally guide the differentiation of cells, promote the proliferation of the cells, reduce the probability of wound infection and promote the healing speed of the wound.
The task of the invention is completed by the following steps:
A) preparing an electrostatic spinning solution, and putting a first mixture and a second mixture which are used as spinning raw materials into a solvent for dissolving to obtain the electrostatic spinning solution, wherein: the first mixture is a mixture of poly-glycerol sebacate and polycaprolactone according to the mass ratio, and the second mixture is a mixture of poly-hexamethylene biguanide hydrochloride and poly-glycerol sebacate according to the mass ratio;
B) preparing a finished product, putting the electrostatic spinning solution obtained in the step A) into an injector of electrostatic spinning equipment by adopting an electrostatic spinning method, applying a high-voltage electrostatic field between an injection needle head serving as a spinning nozzle of the injector and a grounded receiving device, keeping a distance between the injection needle head and the receiving device, enabling the electrostatic spinning solution from the injection needle head to pass through the high-voltage electrostatic field and be emitted onto the receiving device in a jet flow form under the state of controlling the flow rate of the electrostatic spinning solution, forming a nanofiber film along with continuous accumulation on the receiving device, and drying to obtain the high-elasticity antibacterial nanofiber film for wound healing.
In a specific embodiment of the present invention, the mass percentage of the solvent in the electrospinning liquid in step a) is 89.5 to 93.9%, the mass percentage of the mixture of the poly (glycerol sebacate) and the polycaprolactone in the electrospinning liquid is 2.1 to 4.5%, and the mass percentage of the mixture of the poly (hexamethylene biguanide hydrochloride) and the poly (glycerol sebacate) in the electrospinning liquid is 4 to 6%.
In another specific embodiment of the present invention, the mass ratio of the poly (glycerol sebacate) to the polycaprolactone is 1: 3-5, and the mass ratio of the poly (hexamethylene biguanide hydrochloride) to the poly (glycerol sebacate) is 1: 2-50; the solvent is hexafluoroisopropanol with the purity of more than 99.5 percent.
In another specific embodiment of the present invention, the concentration of polycaprolactone is 5-15% by mass; the mass percentage concentration of the polysebacic acid glycerol ester is 1-5%; the mass percentage concentration of the polyhexamethylene biguanide hydrochloride is 0.1-0.5%.
In a further specific embodiment of the present invention, the high voltage electrostatic field applied between the injection needle and the grounding means in step B) is 15-30 kv.
In yet another embodiment of the invention, said keeping of the distance between the injection needle and the receiving means in step B) is keeping the distance between 10-20 cm.
In a more specific embodiment of the present invention, the controlling of the electrospinning liquid flow rate in the step B) is to control the electrospinning liquid flow rate to be 0.5 to 2 mL/h.
In a further embodiment of the invention, the receiving device in step B) is a motorized drum receiver.
In still another specific embodiment of the present invention, the drying temperature of the drying device in step B) is 40-100 ℃, and the drying time is 1-5 h.
In yet another specific embodiment of the present invention, the drying device is an oven.
The technical scheme provided by the invention has the technical effects that: materials with different effects are compounded by utilizing electrostatic spinning to obtain a dressing product with a multifunctional effect, the elasticity of the fiber membrane is improved by introducing a degradable material, namely polypropylene sebacate, and the mechanical strength of the fiber membrane is improved by adding polycaprolactone; besides the two components of the poly-glycerol sebacate and the polycaprolactone, the poly-hexamethylene biguanide hydrochloride is added in the preparation process of the nano-fiber, the poly-hexamethylene biguanide hydrochloride attached to the fiber has an antibacterial effect, wound infection is avoided, and meanwhile, the fibrous structure simulates an extracellular matrix structure, so that proliferation and differentiation of cells can be guided, and the healing of the wound is promoted.
Drawings
Fig. 1 is an SEM image of a high-elasticity antibacterial nanofiber membrane for wound healing obtained in an example of the present invention.
Fig. 2 is a graph comparing tensile strength of the highly elastic antibacterial nanofiber films for wound healing obtained in examples 1 to 3 of the present invention.
Detailed Description
Example 1:
A) preparing an electrostatic spinning solution, and putting a first mixture and a second mixture which are used as spinning raw materials into a solvent for dissolving to obtain the electrostatic spinning solution, wherein: the first mixture is a mixture formed by mixing poly (glycerol sebacate) and polycaprolactone according to the mass ratio of 1: 5, the mass percentage of the mixture in the electrospinning liquid is 2.1%, the second mixture is a mixture formed by mixing poly (hexamethylene biguanide hydrochloride) and poly (glycerol sebacate) according to the mass ratio of 1: 26, the mass percentage of the mixture in the electrospinning liquid is 4%, the purity of the solvent, namely hexafluoroisopropanol, is more than 99.5%, the mass percentage of the solvent in the electrospinning liquid is 93.9%, the mass percentage concentration of polycaprolactone is 5%, the mass percentage concentration of poly (glycerol sebacate) is 1%, and the mass percentage concentration of poly (hexamethylene biguanide hydrochloride) is 0.1%;
B) preparing a finished product, placing the electrostatic spinning solution obtained in the step A) into an injector of electrostatic spinning equipment by adopting an electrostatic spinning method, applying a 30kv high-voltage electrostatic field between an injection needle serving as a spinning nozzle of the injector and a grounded receiving device, namely an electric drum type receiver, keeping a distance of 10cm between the injection needle and the receiving device, namely the injection needle and the electric drum type receiver, enabling the electrostatic spinning solution from the injection needle to be emitted onto the receiving device in a jet flow form through the high-voltage electrostatic field under the condition of controlling the flow rate of the electrostatic spinning solution to be 2ml/h, forming a nanofiber film along with continuous accumulation on the receiving device, namely the electric drum type receiver, drying the nanofiber film through a drying device, namely an oven at 70 ℃ and 3h to obtain the high-elasticity antibacterial nanofiber film for healing wounds shown in figure 1, the electrostatic spinning in this step is carried out at room temperature, i.e. the temperature during the electrostatic spinning is room temperature.
Cutting the electrostatic spinning fiber film into a circular membrane with the diameter of 6mm, placing the circular membrane under an ultraviolet lamp for irradiating for 15min, transferring the circular membrane onto a culture solution inoculated with escherichia coli for testing (namely the culture solution inoculated with the cultured escherichia coli) by using tweezers to perform an antibacterial test, wherein the culture temperature is 35-39 ℃, the culture time is 18-24h, and then observing the size of a bacteriostatic zone. The tensile strength of the high-elasticity antibacterial nanofiber film for wound healing obtained in this example is shown in fig. 2.
Example 2:
A) preparing an electrostatic spinning solution, and putting a first mixture and a second mixture which are used as spinning raw materials into a solvent for dissolving to obtain the electrostatic spinning solution, wherein: the first mixture is a mixture formed by mixing poly (glycerol sebacate) and polycaprolactone according to the mass ratio of 1: 4, the mass percentage of the mixture in the electrospinning liquid is 3%, the second mixture is a mixture formed by mixing poly (hexamethylene biguanide hydrochloride) and poly (glycerol sebacate) according to the mass ratio of 1: 2, the mass percentage of the mixture in the electrospinning liquid is 5%, the purity of the solvent, namely hexafluoroisopropanol, is more than 99.5%, the mass percentage of the solvent in the electrospinning liquid is 92%, the mass percentage concentration of polycaprolactone is 15%, the mass percentage concentration of poly (glycerol sebacate) is 3%, and the mass percentage concentration of poly (hexamethylene biguanide hydrochloride) is 0.3%;
B) preparing a finished product, placing the electrostatic spinning solution obtained in the step A) into an injector of electrostatic spinning equipment by adopting an electrostatic spinning method, a 30kv high-voltage electrostatic field is applied between an injection needle of a spinneret serving as an injector and a grounded receiving device, namely an electric drum type receiver, and the distance between the injection needle and the receiving device (i.e. the injection needle and the motorized roller type receiver) is kept at 10cm, under the condition of controlling the flow rate of the electrostatic spinning solution to be 2ml/h, the electrostatic spinning solution from the injection needle head is jetted to a receiving device (namely to an electric drum type receiver) in a jet flow form through a high-voltage electrostatic field, a nanofiber film is formed along with continuous accumulation on the receiving device (namely the electric drum type receiver), and then the nanofiber film is dried by a drying device (namely an oven at 70 ℃ for 3 h) to obtain the high-elasticity antibacterial nanofiber film for wound healing shown in figure 1. The electrostatic spinning in this step is carried out at room temperature, i.e. the temperature during the electrostatic spinning is room temperature.
Cutting the electrostatic spinning fiber film into a circular membrane with the diameter of 6mm, placing the circular membrane under an ultraviolet lamp for irradiating for 15min, transferring the circular membrane onto a culture solution inoculated with escherichia coli for testing (namely the culture solution inoculated with the cultured escherichia coli) by using tweezers to perform an antibacterial test, wherein the culture temperature is 35-39 ℃, the culture time is 18-24h, and then observing the size of a bacteriostatic zone. The tensile strength of the high-elasticity antibacterial nanofiber film for wound healing obtained in this example is shown in fig. 2.
Example 3:
A) preparing an electrostatic spinning solution, and putting a first mixture and a second mixture which are used as spinning raw materials into a solvent for dissolving to obtain the electrostatic spinning solution, wherein: the first mixture is a mixture formed by mixing poly (trimethylene sebacate) and polycaprolactone according to the mass ratio of 1: 3, the mass percentage of the mixture in the electrospinning liquid is 4.5%, the second mixture is a mixture formed by mixing poly (hexamethylene biguanide hydrochloride) and poly (trimethylene sebacate) according to the mass ratio of 1: 50, the mass percentage of the mixture in the electrospinning liquid is 6%, the purity of the solvent, namely hexafluoroisopropanol, is more than 99.5%, the mass percentage of the solvent in the electrospinning liquid is 89.5%, the mass percentage concentration of polycaprolactone is 10%, the mass percentage concentration of poly (trimethylene sebacate) is 5%, and the mass percentage concentration of poly (hexamethylene biguanide hydrochloride) is 0.5%;
B) preparing a finished product, placing the electrostatic spinning solution obtained in the step A) into an injector of electrostatic spinning equipment by adopting an electrostatic spinning method, a 30kv high-voltage electrostatic field is applied between an injection needle of a spinneret serving as an injector and a grounded receiving device, namely an electric drum type receiver, and the distance between the injection needle and the receiving device (i.e. the injection needle and the motorized roller type receiver) is kept at 10cm, under the condition of controlling the flow rate of the electrostatic spinning solution to be 2ml/h, the electrostatic spinning solution from the injection needle head is jetted to a receiving device (namely to an electric drum type receiver) in a jet flow form through a high-voltage electrostatic field, a nanofiber film is formed along with continuous accumulation on the receiving device (namely the electric drum type receiver), and then the nanofiber film is dried by a drying device (namely an oven at 70 ℃ for 3 h) to obtain the high-elasticity antibacterial nanofiber film for wound healing shown in figure 1. The electrostatic spinning in this step is carried out at room temperature, i.e. the temperature during the electrostatic spinning is room temperature.
Cutting the electrostatic spinning fiber film into a circular membrane with the diameter of 6mm, placing the circular membrane under an ultraviolet lamp for irradiating for 15min, transferring the circular membrane onto a culture solution inoculated with the test escherichia coli (namely the culture solution inoculated with the test escherichia coli) by using tweezers to perform an antibacterial test, wherein the culture temperature is 35-39 ℃, the culture time is 18-24h, and then observing the size of a bacteriostatic zone, wherein the size of the bacteriostatic zone is obviously larger than that of other samples. The tensile strength of the high-elasticity antibacterial nanofiber film for wound healing obtained in this example is shown in fig. 2.
Table 1 is a table comparing the zones of inhibition of examples 1 to 3 of the present invention
Claims (10)
1. A preparation method of a high-elasticity antibacterial nanofiber film for wound healing is characterized by comprising the following steps:
A) preparing an electrostatic spinning solution, and putting a first mixture and a second mixture which are used as spinning raw materials into a solvent for dissolving to obtain the electrostatic spinning solution, wherein: the first mixture is a mixture of poly-glycerol sebacate and polycaprolactone according to the mass ratio, and the second mixture is a mixture of poly-hexamethylene biguanide hydrochloride and poly-glycerol sebacate according to the mass ratio;
B) preparing a finished product, putting the electrostatic spinning solution obtained in the step A) into an injector of electrostatic spinning equipment by adopting an electrostatic spinning method, applying a high-voltage electrostatic field between an injection needle head serving as a spinning nozzle of the injector and a grounded receiving device, keeping a distance between the injection needle head and the receiving device, enabling the electrostatic spinning solution from the injection needle head to pass through the high-voltage electrostatic field and be emitted onto the receiving device in a jet flow form under the state of controlling the flow rate of the electrostatic spinning solution, forming a nanofiber film along with continuous accumulation on the receiving device, and drying to obtain the high-elasticity antibacterial nanofiber film for wound healing.
2. The method for preparing a highly elastic antibacterial nanofiber membrane for wound healing according to claim 1, wherein the solvent in step a) accounts for 89.5-93.9% by mass of the electrospinning solution, the mixture of the polytrimethylene sebacate and the polycaprolactone in the electrospinning solution accounts for 2.1-4.5% by mass, and the mixture of the polyhexamethylene biguanide hydrochloride and the polytrimethylene sebacate in the electrospinning solution accounts for 4-6% by mass.
3. The method for preparing a highly elastic antibacterial nanofiber membrane for wound healing according to claim 1 or 2, wherein the mass ratio of the poly (glycerol sebacate) to the polycaprolactone is 1: 3-5, and the mass ratio of the poly (hexamethylene biguanide hydrochloride) to the poly (glycerol sebacate) is 1: 2-50; the solvent is hexafluoroisopropanol with the purity of more than 99.5 percent.
4. The method for preparing a high-elasticity antibacterial nanofiber film for wound healing according to claim 3, wherein the mass percent concentration of polycaprolactone is 5-15%; the mass percentage concentration of the polysebacic acid glycerol ester is 1-5%; the mass percentage concentration of the polyhexamethylene biguanide hydrochloride is 0.1-0.5%.
5. The method for preparing a highly elastic antibacterial nanofiber membrane for wound healing as claimed in claim 1, wherein the high voltage electrostatic field applied between the injection needle and the grounding device in step B) is 15-30 kv.
6. The method for preparing a highly elastic antibacterial nanofiber membrane for wound healing as claimed in claim 1, wherein the distance between the injection needle and the receiving device in step B) is kept at 10-20 cm.
7. The method for preparing a highly elastic antibacterial nanofiber membrane for wound healing as claimed in claim 1, wherein the controlling of the flow rate of the electrospinning solution in step B) is to control the flow rate of the electrospinning solution to 0.5-2 mL/h.
8. The method for preparing a highly elastic antibacterial nanofiber membrane for wound healing as claimed in claim 1, wherein the receiving device in step B) is an electric drum receiver.
9. The method for preparing a high-elasticity antibacterial nanofiber film for wound healing according to claim 1, wherein the drying temperature of the drying device in the step B) is 40-100 ℃, and the drying time is 1-5 h.
10. The method for preparing the high-elasticity antibacterial nanofiber film for wound healing according to claim 1, wherein the drying device is an oven.
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| CN115418796A (en) * | 2022-09-14 | 2022-12-02 | 百达联康生物科技(深圳)有限公司 | Antibacterial fiber membrane and preparation method thereof |
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