CN114018874B - Adipose tissue source exosome detection device and detection method thereof - Google Patents
Adipose tissue source exosome detection device and detection method thereof Download PDFInfo
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- CN114018874B CN114018874B CN202111325299.4A CN202111325299A CN114018874B CN 114018874 B CN114018874 B CN 114018874B CN 202111325299 A CN202111325299 A CN 202111325299A CN 114018874 B CN114018874 B CN 114018874B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/55—Specular reflectivity
- G01N21/552—Attenuated total reflection
- G01N21/553—Attenuated total reflection and using surface plasmons
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
Abstract
The invention discloses an adipose tissue source exosome detection device and a detection method thereof, wherein the detection device comprises a multilayer centrifugal component; the multi-layer centrifugal assembly comprises a shell arranged between a base and a top seat; a first centrifugal cylinder, a second centrifugal cylinder and a third centrifugal cylinder are arranged in the shell; the first centrifugal cylinder and the second centrifugal cylinder are movably connected through a bearing, and the second centrifugal cylinder and the third centrifugal cylinder are movably connected through a bearing; the outer circle of the first bearing is embedded on the inner wall of the top of the second centrifugal cylinder, and the bottom of the first centrifugal cylinder is fixed on the inner circle of the first bearing; the outer circle of the second bearing is embedded on the inner wall of the top of the third centrifugal cylinder, and the bottom of the second centrifugal cylinder is fixed on the inner circle of the second bearing; a driven gear is sleeved on the upper part of the outer ring of the second centrifugal cylinder and is meshed and connected with the transmission gear; the transmission gear is fixed on the transmission shaft, and the transmission shaft is rotationally connected with the third motor; the upper end and the lower end of a transmission gear positioned on the transmission shaft are respectively provided with an upper shifting piece and a lower shifting piece; the top of the transmission shaft is provided with a locking piece.
Description
Technical Field
The invention belongs to the technical field of medical equipment, and particularly relates to an adipose tissue source exosome detection device and a detection method thereof.
Background
Exosomes (exosomes) are membrane vesicles of 30-150nm diameter secreted by a variety of cells, derived from multivesicular bodies. Various cells can secrete exosomes under normal and pathological states, mainly come from a multivesicular body formed by invagination of lysosome particles in donor cells, and are released into extracellular matrix after fusion of multivesicular body outer membranes and cell membranes. The origin of the exosome determines its envelope of cytoplasmic components of the donor cell, including a variety of unique nucleic acid molecules, protein and lipid components, and also determines the functional specificity of the exosome. The exosome is widely present in various body fluids, carries specific cytoplasmic components of donor cells, and can realize long-distance signal transmission along with peripheral blood circulation to a distant target organ, so that a brand-new tissue homeostasis regulation and intercellular information transmission system is formed, and the exosome is a new hotspot of current basic and clinical researches.
Exosomes are considered as specifically secreted membrane vesicles, involved in intercellular communication, and the precise molecular mechanisms involved in exosome secretion and uptake, their "cargo" transport, and intercellular information regulation remain largely studied.
Scientists at the Joslin diabetes center, harvard university, published a paper in nature, and found that adipose tissue is a major source of circulating exosome micrornas, and that these micrornas can transmit information through exosome forms, regulating gene transcription and translation and biological functions of distant organs. The novel intercellular information communication mode makes people realize that adipose tissues are used as important endocrine organs of the body, and play an important regulation and control effect on the aspects of regulating homeostasis of the body and acute emergency stimulation response. Provides a new idea for the individual treatment of chronic metabolic syndrome such as diabetes, obesity and the like and acute critical diseases such as sepsis, acute respiratory infection and the like.
However, the existing detection device for the adipose tissue-derived exosome, especially the separation device thereof, needs a very precise instrument, has high manufacturing cost, is too delicate and complex to operate, and has great limitations in usability and operability.
Disclosure of Invention
The present invention is directed to an adipose tissue-derived exosome detection apparatus and a detection method thereof, which solve or improve the above-mentioned problems.
In order to achieve the purpose, the invention adopts the technical scheme that:
in one aspect, an adipose tissue source exosome detecting device and a detecting method thereof comprise a multi-layer centrifugal assembly for separating each component in an adipose tissue source; the multi-layer centrifugal assembly comprises a shell arranged between a base and a top seat;
a first centrifugal cylinder, a second centrifugal cylinder and a third centrifugal cylinder which are sequentially communicated from top to bottom are arranged in the shell; the top of the first centrifugal cylinder is rotationally connected with a second motor, and the third centrifugal cylinder is rotationally connected with the first motor; the first centrifugal cylinder is movably connected with the second centrifugal cylinder through a first bearing, and the second centrifugal cylinder is movably connected with the third centrifugal cylinder through a second bearing;
the outer circle of the first bearing is embedded on the inner wall of the top of the second centrifugal cylinder, and the bottom of the first centrifugal cylinder is fixed on the inner circle of the first bearing;
the outer circle of the second bearing is embedded on the inner wall of the top of the third centrifugal cylinder, and the bottom of the second centrifugal cylinder is fixed on the inner circle of the second bearing;
a driven gear is sleeved on the upper part of the outer ring of the second centrifugal cylinder and is meshed and connected with the transmission gear; the transmission gear is fixed on the transmission shaft, and the transmission shaft is rotationally connected with the third motor; the upper end and the lower end of a transmission gear positioned on the transmission shaft are respectively provided with an upper shifting piece and a lower shifting piece; the top of transmission shaft is equipped with the retaining member that is used for locking the transmission shaft rotation.
Further, the first motor, the second motor and the third motor are respectively fixed on the first base, the second base and the third base; the first base and the third base are both fixed on the base, and the second base is fixed on the top seat.
Further, the first centrifugal cylinder is communicated with the first separation pipe, and the second centrifugal cylinder is communicated with the second separation pipe.
Further, the first separation pipe and the second separation pipe are both L-shaped pipes, and one ends, far away from the shell, of the L-shaped pipes are communicated with the first separation tank and the second separation tank respectively.
Further, the first separation tank is in threaded connection with the first separation pipe, and the second separation tank is in threaded connection with the second separation pipe.
Furthermore, a positioning convex ring is arranged at the communicated junction between the first centrifugal cylinder and the second centrifugal cylinder; the inner ring of the positioning convex ring is provided with a screen; a positioning groove is formed between the positioning convex ring and the first bearing; the bottom of the first centrifugal cylinder is embedded in the positioning groove.
Further, the locking piece is fixed on the top seat; the locking piece comprises a limiting rod; the gag lever post is located spacing intracavity, and gag lever post one end outwards extends first connecting portion and with handle fixed connection, and the gag lever post other end extends, passes second connecting portion and imbeds in the locking hole of seting up on the transmission shaft to transmission shaft one side.
Furthermore, the limiting rod is evenly provided with external threads, and the first connecting portion and the second connecting portion are internal threads matched with the external threads of the limiting rod.
Furthermore, a guide block is sleeved on the limiting rod, an annular groove is formed in the side edge, close to the inner wall of the limiting cavity, of the guide block, and a plurality of balls are uniformly arranged in the annular groove; the ball is connected with the inner wall of the limiting cavity in a rolling way; the limiting rod is provided with a spring in a sleeved mode between the guide block and the second connecting portion.
In one aspect, a method for detecting an adipose tissue-derived exosome detection device includes:
s1, guiding the sucked adipose tissue source into a first centrifugal cylinder, sliding the position of a transmission gear until the sucked adipose tissue source is meshed with a driven gear, sliding the positions of an upper stirring sheet and a lower stirring sheet to the upper end and the lower end of the transmission gear to clamp and fix the position of the transmission gear, and simultaneously rotating a handle on a locking piece in the forward direction to screw a limiting rod into a locking hole to lock the state of a transmission shaft;
s2, starting a second motor, carrying out first centrifugation on the adipose tissue source, separating fat in the adipose tissue source, and leading the fat into a first separation tube along with the centrifugal force and storing the fat in a first separation tank;
after separation, the second motor is closed, and the rest adipose tissue source flows into the second separation cylinder under the action of gravity;
s3, the second motor is turned off, the first separating drum stops in a centrifugal state, the handle on the locking piece is rotated reversely, the limiting rod is pulled out of the locking hole, and the transmission shaft is released; starting a third motor to drive a transmission shaft and a transmission gear to rotate, wherein the transmission gear drives a driven gear to select and drives a second centrifugal cylinder to centrifugally move;
after separation, the third motor is closed, the connective tissue is separated by centrifugal motion of the second centrifugal cylinder, and the separated connective tissue is guided into the second separation tube along with the centrifugal force and is stored in the second separation tank;
s4, rotating the handle on the locking piece in the forward direction again, screwing the limiting rod into the locking hole, and locking the state of the transmission shaft; starting a first motor to drive a third separation barrel to centrifugally move until exosomes in the mixed liquid are layered, closing the first motor, and extracting layered exosome concentrated solution by using a needle head;
s5, extracting the extracellular vesicles in the exosome by a precipitation method, and detecting each component in the exosome by a surface plasmon resonance imaging method.
The adipose tissue source exosome detection device and the detection method thereof provided by the invention have the following beneficial effects:
when the device disclosed by the invention is used for separating an adipose tissue source, mixed liquid sequentially flows through the first centrifugal cylinder, the second centrifugal cylinder and the third centrifugal cylinder from top to bottom along with gravity, multi-layer separation of the adipose tissue source is realized under the cooperation of the first motor, the second motor and the third motor, an exosome concentrated solution to be detected is obtained, and each component in a target exosome is further detected and obtained by adopting a surface plasmon resonance imaging method.
Drawings
Fig. 1 is a structural view of an adipose tissue-derived exosome detection apparatus and a detection method thereof.
Fig. 2 shows a communication structure between the first centrifugal cylinder and the second centrifugal cylinder, and between the second centrifugal cylinder and the third centrifugal cylinder.
Fig. 3 is an enlarged view of a point a in fig. 1.
Wherein, 1, a shell; 2. a base; 3. a top seat; 4. a first centrifuge tube; 5. a second centrifuge tube; 6. a third centrifugal cylinder; 7. a first bearing; 8. a second bearing; 9. a first base; 10. a first motor; 11. a second base; 12. a second motor; 13. a third base; 14. a third motor; 15. a drive shaft; 16. a lower shifting sheet; 17. a transmission gear; 18. an upper shifting sheet; 19. a locking member; 20. a first separation tube; 21. a driven gear; 22. a second separation tube; 23. a first separation tank; 24. a second separation tank; 25. positioning a groove; 26. positioning the convex ring; 27. screening a screen; 28. a locking hole; 191. a limiting cavity; 192. a handle; 193. a first connection portion; 194. a second connecting portion; 195. a limiting rod; 196. a guide block; 197. a spring.
Detailed Description
The following description of the embodiments of the present invention is provided to facilitate the understanding of the present invention by those skilled in the art, but it should be understood that the present invention is not limited to the scope of the embodiments, and it will be apparent to those skilled in the art that various changes may be made without departing from the spirit and scope of the invention as defined and defined in the appended claims, and all matters produced by the invention using the inventive concept are protected.
According to one embodiment of the present application, referring to fig. 1-3, the adipose tissue source exudate detection device of the present scheme includes a multi-layered centrifugal assembly for separating components of an adipose tissue source, the multi-layered centrifugal assembly including a housing 1 disposed between a base 2 and a top mount 3.
A first centrifugal cylinder 4, a second centrifugal cylinder 5 and a third centrifugal cylinder 6 which are sequentially communicated from top to bottom are arranged in the shell 1, and the first centrifugal cylinder 4 is used for separating fat with lighter weight.
The second centrifuge bowl 5 is used for separating connective tissue by centrifugation.
The third centrifugal cylinder 6 is used for layering the exosomes in the remaining mixed liquid for facilitating the extraction of the exosomes.
Specifically, the top of the first centrifugal cylinder 4 is rotationally connected with a second motor 12, the third centrifugal cylinder 6 is rotationally connected with a first motor 10, and the second motor 12 and the first motor 10 respectively drive the first centrifugal cylinder 4 and the third centrifugal cylinder 6 to rotate.
The first centrifugal cylinder 4 is movably connected with the second centrifugal cylinder 5 through a first bearing 7, and the second centrifugal cylinder 5 is movably connected with the third centrifugal cylinder 6 through a second bearing 8.
The excircle of the first bearing 7 is embedded on the inner wall of the top of the second centrifugal cylinder 5, and the bottom of the first centrifugal cylinder 4 is fixed on the inner circle of the first bearing 7.
The excircle of the second bearing 8 is embedded on the inner wall of the top of the third centrifugal cylinder 6, and the bottom of the second centrifugal cylinder 5 is fixed on the inner circle of the second bearing 8.
The junction of the first centrifuge tube 4 and the second centrifuge tube 5 is provided with a positioning convex ring 26, the inner ring of the positioning convex ring 26 is provided with a screen 27, and the screen 27 has a certain filtering function and can filter part of objects in the mixed liquid.
A positioning groove 25 is formed between the positioning convex ring 26 and the first bearing 7, the bottom of the first centrifugal cylinder 4 is embedded in the positioning groove 25, and the bottom of the second centrifugal cylinder 5 is embedded in the positioning groove 25 between the second centrifugal cylinder 5 and the third centrifugal cylinder 6.
Namely, the first centrifugal cylinder 4 is embedded in the second centrifugal cylinder 5 through the first bearing 7, and the first centrifugal cylinder 4 and the second centrifugal cylinder 5 can move relatively, and in the state that the second motor 12 is locked, the first centrifugal cylinder 4 is static, and the second centrifugal cylinder 5 can rotate for operation; the third centrifugal cylinder 6 has the same structure, so it is not described again.
The second centrifugal cylinder 5 realizes the rotating operation through the meshing and matching of the driven gear 21 and the transmission gear 17, so that the state of the second centrifugal cylinder 5 needs to be independently locked, a locking piece 19 needs to be additionally arranged, namely the locking piece 19 is further additionally arranged on a transmission shaft to lock the state of the transmission shaft, and further the locking state of the second centrifugal cylinder 5 is increased; when the second centrifugal cylinder 5 is locked, the first centrifugal cylinder 4 and the second centrifugal cylinder 5 can rotate independently, i.e. the first centrifugal cylinder 4, the second centrifugal cylinder 5 and the third centrifugal cylinder 6 can perform independent centrifugal operation without mutual influence.
The specific transmission structure of the second centrifugal cylinder 5 is as follows:
a driven gear 21 is sleeved on the upper part of the outer ring of the second centrifugal cylinder 5, and the driven gear 21 is meshed and connected with the transmission gear 17; the transmission gear 17 is fixed on the transmission shaft 15, and the transmission shaft 15 is rotationally connected with the third motor 14; the upper end and the lower end of a transmission gear 17 positioned on the transmission shaft 15 are respectively provided with an upper shifting piece 18 and a lower shifting piece 16; the top of the driving shaft 15 is provided with a locking member 19 for locking the driving shaft 15 to rotate.
Wherein, the upper shifting piece 18 and the lower shifting piece 16 are connected with the transmission shaft 15 in a damping way, and have larger friction force.
The locking member 19 is fixed on the top seat 3, the locking member 19 comprises a limiting rod 195, the limiting rod 195 is located in the limiting cavity 191, one end of the limiting rod 195 outwards extends through the first connecting portion 193 and is fixedly connected with the handle 192, and the other end of the limiting rod 195 extends towards one side of the transmission shaft 15, extends through the second connecting portion 194 and is embedded into the locking hole 28 formed in the transmission shaft 15.
In order to facilitate the insertion of the stopper 195 into the locking hole 28, the locking hole 28 may be provided in plurality in the circumferential direction.
External threads are uniformly formed on the limiting rod 195, and the first connecting portion 193 and the second connecting portion 194 are internal threads matched with the external threads of the limiting rod 195.
The limiting rod 195 is sleeved with a guide block 196, the guide block 196 is used for auxiliary guiding, an annular groove is formed in the side edge, close to the inner wall of the limiting cavity 191, of the guide block 196, a plurality of balls are uniformly arranged in the annular groove and are connected with the inner wall of the limiting cavity 191 in a rolling mode, the rotating capacity of the guide block 196 is improved, friction force is reduced, and meanwhile the guide block 196 can be conveniently screwed in and out along with the transmission shaft 15.
A spring 197 is sleeved on the limiting rod 195 between the guide block 196 and the second connecting portion 194, and the spring 197 has a certain cushioning effect.
The method comprises the steps of locking and releasing states during specific operation;
in the locking state, the position of the transmission gear 17 is slid until the transmission gear 17 is meshed and connected with the driven gear 21, the positions of the upper shifting piece 18 and the lower shifting piece 16 are slid to the upper end and the lower end of the transmission gear 17 to clamp and fix the position of the transmission gear 17, and meanwhile, the handle 192 on the locking piece 19 is rotated in the positive direction to screw the limiting rod 195 into the locking hole 28 to lock the state of the transmission shaft 15;
in the releasing state, the handle 192 on the locking piece 19 is rotated reversely, the limiting rod 195 is pulled away from the locking hole 28, the transmission shaft 15 is released, the third motor 14 is started to drive the transmission shaft 15 and the transmission gear 17 to rotate, the transmission gear 17 drives the driven gear 21 to select, and the second centrifugal cylinder 5 is driven to do centrifugal motion.
The first motor 10, the second motor 12 and the third motor 14 are respectively fixed on the first base 9, the second base 11 and the third base 13; the first base 9 and the third base 13 are both fixed on the base 2, and the second base 11 is fixed on the top base 3.
The first centrifuge bowl 4 communicates with a first separator tube 20, and the second centrifuge bowl 5 communicates with a second separator tube 22.
The first separation pipe 20 and the second separation pipe 22 are both L-shaped pipes, and one ends of the L-shaped pipes, which are far away from the shell 1, are respectively communicated with a first separation tank 23 and a second separation tank 24.
The first separating tank 23 is in threaded connection with the first separating pipe 20, the second separating tank 24 is in threaded connection with the second separating tank 22, a crushing device can be further arranged in the first separating tank 23 and the second separating tank 24, and the crushing device can select a nano fat incubator to obtain nano fat particles and nano connective tissue particles.
According to an embodiment of the application, the detection method of the adipose tissue-derived exosome detection device of the present scheme comprises the following steps:
s1, guiding the sucked adipose tissue source into the first centrifugal cylinder 4, sliding the position of the transmission gear 17 until the position is meshed with the driven gear 21, sliding the positions of the upper shifting sheet 18 and the lower shifting sheet 16 to the upper end and the lower end of the transmission gear 17 to clamp and fix the position of the transmission gear 17, and simultaneously, rotating the handle 192 on the locking piece 19 in the positive direction to screw the limiting rod 195 into the locking hole 28 to lock the state of the transmission shaft 15;
s2, starting the second motor 12, centrifuging the adipose tissue source for the first time, separating fat in the adipose tissue source, and leading the fat into the first separation tube 20 along with the centrifugal force and storing the fat in the first separation tank 23;
after the separation is finished, the second motor 12 is closed, and the rest adipose tissue source flows into the second separation cylinder under the action of gravity;
s3, turning off the second motor 12, stopping the centrifugal state of the first separating drum, reversely rotating the handle 192 on the locking piece 19, drawing the limiting rod 195 away from the locking hole 28, and releasing the transmission shaft 15; starting a third motor 14 to drive a transmission shaft 15 and a transmission gear 17 to rotate, wherein the transmission gear 17 drives a driven gear 21 to select and drives a second centrifugal cylinder 5 to do centrifugal motion;
after the separation is finished, the third motor 14 is turned off, the second centrifugal cylinder 5 carries out centrifugal motion to separate out connective tissues, and the separated connective tissues are guided into a second separation pipe 22 along with the centrifugal force and stored in a second separation tank 23;
s4, rotating the handle 192 on the locking piece 19 forward again, screwing the limiting rod 195 into the locking hole 28, and locking the state of the transmission shaft 15; starting the first motor 10 to drive the third separation barrel to do centrifugal motion until exosomes in the mixed liquid are layered, closing the first motor 10, and extracting the layered exosome concentrated solution by using a needle head;
s5, extracting extracellular vesicles in the exosomes by using a precipitation method, and detecting each component in the exosomes by using a surface plasmon resonance imaging method.
When the device of the invention separates an adipose tissue source, mixed liquid flows through a first centrifugal cylinder 4, a second centrifugal cylinder 5 and a third centrifugal cylinder 6 from top to bottom in sequence along with gravity, multi-layer separation of the adipose tissue source is realized under the cooperation of a first motor 10, a second motor 12 and a third motor 14, an exosome concentrated solution to be detected is obtained, and each component in a target exosome is further detected and obtained by adopting a surface plasma resonance imaging method.
While the embodiments of the invention have been described in detail in connection with the accompanying drawings, it is not intended to limit the scope of the invention. Various modifications and changes may be made by those skilled in the art without inventive step within the scope of the appended claims.
Claims (7)
1. An adipose tissue-derived exosome detection device, characterized in that: comprising a multi-layer centrifuge assembly for separating components of a source of adipose tissue; the multi-layer centrifugal assembly comprises a shell arranged between a base and a top seat;
a first centrifugal cylinder, a second centrifugal cylinder and a third centrifugal cylinder which are sequentially communicated from top to bottom are arranged in the shell; the top of the first centrifugal cylinder is rotationally connected with a second motor, and the third centrifugal cylinder is rotationally connected with the first motor; the first centrifugal cylinder and the second centrifugal cylinder are movably connected through a first bearing, and the second centrifugal cylinder and the third centrifugal cylinder are movably connected through a second bearing;
the outer circle of the first bearing is embedded on the inner wall of the top of the second centrifugal cylinder, and the bottom of the first centrifugal cylinder is fixed on the inner circle of the first bearing;
the outer circle of the second bearing is embedded on the inner wall of the top of the third centrifugal cylinder, and the bottom of the second centrifugal cylinder is fixed on the inner circle of the second bearing;
a driven gear is sleeved on the upper part of the outer ring of the second centrifugal cylinder and is meshed and connected with the transmission gear; the transmission gear is fixed on the transmission shaft, and the transmission shaft is rotationally connected with the third motor; the upper end and the lower end of a transmission gear positioned on the transmission shaft are respectively provided with an upper shifting piece and a lower shifting piece; the top of the transmission shaft is provided with a locking piece for locking the transmission shaft to rotate;
a positioning convex ring is arranged at the communicated junction between the first centrifugal cylinder and the second centrifugal cylinder; the inner ring of the positioning convex ring is provided with a screen; a positioning groove is formed between the positioning convex ring and the first bearing; the bottom of the first centrifugal cylinder is embedded in the positioning groove; the bottom of the second centrifugal cylinder is embedded in a positioning groove between the second centrifugal cylinder and the third centrifugal cylinder;
the first centrifugal cylinder is communicated with the first separation pipe, and the second centrifugal cylinder is communicated with the second separation pipe;
the first separation pipe and the second separation pipe are both L-shaped pipes, one end, away from the shell, of the first separation pipe, which is an L-shaped pipe, is communicated with the first separation tank, and one end, away from the shell, of the second separation pipe, which is an L-shaped pipe, is communicated with the second separation tank.
2. The adipose tissue-derived exosome detection device according to claim 1, characterized in that: the first motor, the second motor and the third motor are respectively fixed on the first base, the second base and the third base; the first base and the third base are fixed on the base, and the second base is fixed on the top seat.
3. The adipose tissue-derived exosome detection device according to claim 1, characterized in that: the first separation tank is in threaded connection with the first separation pipe, and the second separation tank is in threaded connection with the second separation pipe.
4. The adipose tissue-derived exosome detection device according to claim 1, characterized in that: the locking piece is fixed on the top seat; the locking piece comprises a limiting rod; the limiting rod is located in the limiting cavity, one end of the limiting rod extends outwards to penetrate through the first connecting portion and is fixedly connected with the handle, and the other end of the limiting rod extends towards one side of the transmission shaft, penetrates through the second connecting portion and is embedded into the locking hole formed in the transmission shaft.
5. The adipose tissue-derived exosome detection device according to claim 4, characterized in that: the limiting rod is evenly provided with external threads, and the first connecting portion and the second connecting portion are internal threads matched with the external threads of the limiting rod.
6. The adipose tissue source exosome detection device according to claim 4, characterized in that: the limiting rod is sleeved with a guide block, an annular groove is formed in the side edge, close to the inner wall of the limiting cavity, of the guide block, and a plurality of balls are uniformly arranged in the annular groove; the ball is connected with the inner wall of the limiting cavity in a rolling way; and a spring is sleeved on the limiting rod between the guide block and the second connecting part.
7. Use of an adipose tissue source exosome detection device according to any one of claims 1 to 6, characterized in that it comprises:
s1, guiding the sucked adipose tissue source into a first centrifugal cylinder, sliding the position of a transmission gear until the position is meshed with a driven gear, sliding the positions of an upper plectrum and a lower plectrum to the upper end and the lower end of the transmission gear to clamp and fix the position of the transmission gear, and simultaneously, rotating a handle on a locking piece in the forward direction, screwing a limiting rod into a locking hole, and locking the state of a transmission shaft;
s2, starting a second motor, carrying out first centrifugation on the adipose tissue source, separating fat in the adipose tissue source, and leading the fat into a first separation tube along with the centrifugal force and storing the fat in a first separation tank;
after the separation is finished, the second motor is closed, and the rest adipose tissue source flows into the second separation cylinder under the action of gravity;
s3, the second motor is turned off, the first separating drum stops in a centrifugal state, the handle on the locking piece is rotated reversely, the limiting rod is pulled out of the locking hole, and the transmission shaft is released; starting a third motor to drive the transmission shaft and the transmission gear to rotate, wherein the transmission gear drives the driven gear to select and drive the second centrifugal cylinder to do centrifugal motion;
after the separation is finished, the third motor is closed, the connective tissue is separated by centrifugal motion of the second centrifugal cylinder, and the separated connective tissue is guided into the second separation tube along with the centrifugal force and is stored in the second separation tank;
s4, rotating the handle on the locking piece in the forward direction again, screwing the limiting rod into the locking hole, and locking the state of the transmission shaft; and starting the first motor to drive the third separation barrel to do centrifugal motion until the exosomes in the mixed liquid are layered, closing the first motor, and extracting the layered exosome concentrated solution by using the needle head.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN208373513U (en) * | 2018-04-18 | 2019-01-15 | 河北工业职业技术学院 | A kind of soil test sifter |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6589155B2 (en) * | 2001-04-09 | 2003-07-08 | Medtronic, Inc. | Miniaturized blood centrifuge having side mounted motor with belt drive |
| CN202877097U (en) * | 2012-09-21 | 2013-04-17 | 由旭平 | High-efficiency separating screen |
| CN108607693B (en) * | 2015-03-23 | 2021-04-13 | 唐凌霄 | Direct-drive centrifugal separation equipment |
| CN104906826A (en) * | 2015-05-26 | 2015-09-16 | 清华大学 | Sampling device and method for material flows of each outlet of multistage centrifugal extractor |
| CN206833560U (en) * | 2017-10-26 | 2018-01-02 | 钟日钊 | A kind of three leafing core type Coin cleaning-sorting machines |
| CN108798990A (en) * | 2018-08-04 | 2018-11-13 | 成都蒲江珂贤科技有限公司 | A kind of large torque Windmill rotary device and spinning solution that wind energy utilization is high |
| CN209442968U (en) * | 2019-01-20 | 2019-09-27 | 王碧 | A kind of stem cell separator |
| CN211613446U (en) * | 2019-12-12 | 2020-10-02 | 沈阳品川冶金材料有限公司 | Material screening device for chemical production |
| CN213000610U (en) * | 2020-06-02 | 2021-04-20 | 东莞九头牛环保工业设备有限公司 | Solid-liquid separation filter |
| CN112742100A (en) * | 2020-12-03 | 2021-05-04 | 淮阴师范学院 | Intestinal flora extraction element |
| CN214151832U (en) * | 2020-12-31 | 2021-09-07 | 沈阳中钞信达金融设备有限公司 | Gear shifting and unlocking device |
| CN112705317A (en) * | 2021-01-26 | 2021-04-27 | 杭州新瀚灸道科技有限公司 | Moxa production device and process |
| CN113462546A (en) * | 2021-07-05 | 2021-10-01 | 梁明 | Microbial separation verifying attachment |
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| CN208373513U (en) * | 2018-04-18 | 2019-01-15 | 河北工业职业技术学院 | A kind of soil test sifter |
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